CN114292782B - Bacterium for strengthening facultative FMBR (anaerobic fermentation and fermentation) pesticide wastewater treatment process - Google Patents
Bacterium for strengthening facultative FMBR (anaerobic fermentation and fermentation) pesticide wastewater treatment process Download PDFInfo
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Abstract
The invention discloses a bacterium for strengthening a facultative FMBR pesticide wastewater treatment process, wherein the strain is classified and named as rhodococcus biennis @Rhodococcuselectrodiphilus) TN-3 is preserved in China general microbiological culture collection center with a preservation number of CGMCC No.23936 to solve the defects of the prior art, and the strain can strengthen the process efficiency of treating pesticide wastewater by facultative FMBR, effectively remove organic matters and total nitrogen in the wastewater, reduce the water treatment cost, improve the water treatment efficiency and solve the problem of difficult treatment of the pesticide wastewater. Has practical application value and wide market prospect.
Description
Technical Field
The invention relates to a water pollution control and wastewater treatment technology, which can degrade phenolic organic pollutants in pesticide wastewater and denitrify bacteria and strengthen facultative FMBR to treat the pesticide wastewater.
Background
The yield of agricultural chemicals in China is in the front of the world. The annual waste water amount discharged by the national pesticide production enterprises is about 1.5 hundred million tons, and mainly comprises pesticide synthesis production discharge water, product refining washing water, workshop and equipment washing water and the like. Wherein the treated water accounts for 7% of the total amount, and the treated water accounts for 1% of the treated water.
The wastewater discharged in the pesticide production and use process is complex in quality due to various pesticide varieties, and is mainly characterized in that: the concentration of pollutants is high, and the COD can reach tens of thousands of milligrams per liter; the toxicity is high, and besides pesticides and intermediates, the wastewater also contains harmful substances such as phenol, arsenic, mercury and the like and a plurality of substances which are difficult to biodegrade; malodor and irritation to human respiratory tract and mucous membrane; the water quality and the water quantity are unstable. The method for treating the pesticide wastewater mainly comprises distillation, extraction, adsorption, precipitation, concentration and incineration, oxidation, biochemical, reverse osmosis, active carbon-biomembrane method and the like. At present, the high-toxicity and high-residue pesticides in various countries are generally reduced, the high-efficiency and low-residue pesticides are increasingly applied and developed towards the direction of biological pesticides, which is a fundamental way for preventing and controlling pesticide wastewater pollution.
Numerous studies have shown that microorganisms such as fungi, bacteria, algae, etc. have a very good degrading effect on pesticides. The biomembrane process fixes microbial cells on the filler, and the microorganisms adhere to the filler for growth and propagation, so that membranous biological sludge is formed on the microbial cells. Compared with the conventional activated sludge method, the biological membrane has the advantages of large biological volume concentration, long survival generation, various microorganism types and the like, and is especially suitable for the application of special bacteria in a wastewater system.
In China, most pesticide manufacturers build biochemical treatment devices, but at present, no pesticide manufacturers can obtain ideal treatment effects. Therefore, research on biochemical treatment of such wastewater is necessary.
Disclosure of Invention
In order to solve the defects of the prior art, the project provides a denitrifying bacterium capable of degrading phenolic organic pollutants in pesticide wastewater, can effectively remove the phenolic organic pollutants and total nitrogen in the wastewater, reduces the water treatment cost, improves the water treatment efficiency, and solves the problem that the pesticide wastewater is difficult to treat.
According to domestic market demands, the efficient composite flora which mainly takes facultative anaerobe (up to 80%) is successfully established and utilized in facultative FMBR for degrading pesticide wastewater, and compared with the traditional FMBR, the bacterial flora is added with strain TN-3 which is screened in a laboratory and is used for efficiently degrading phenolic organic pollutants in the pesticide wastewater and denitrifying, so that the aeration quantity is reduced, and a certain energy consumption is reduced.
The technical scheme adopted by the invention is as follows:
a strain for efficiently degrading phenolic organic pollutants in pesticide wastewater and denitrifying the same is classified and named as Rhodococcus bisporus (Rhodococcus) TN-3, and is preserved in China center for type culture Collection, address: the preservation number of the Beijing city Chaoyang area North Chen Xili No. 1 and 3 is: CGMCC No.23936.
The strain is obtained by screening from the sediment of a river in a salt city, and the specific screening method is as follows:
50mL of river mud from a river in the salt city was added using 0.300g/L of phenol solution and 1g of sodium chloride as a screening medium. After 30d, the phenol was degraded to 0.010g/L. Inoculating the supernatant to a denitrification culture medium (0.300 g/L phenol, 1.530 g/L potassium nitrate, 3.6g/L glucose, 0.2g/L potassium dihydrogen phosphate, 0.2g/L magnesium sulfate heptahydrate, 2g calcium carbonate, 1mL/L trace element solution (80 mg zinc chloride, 20mg anhydrous copper sulfate, 20mg boric acid, 100mg ferrous sulfate heptahydrate), 1000mL water, 7-7.5) under aseptic condition, culturing, and measuring NH every 12h 4 + -N、NO 3 - -N, total nitrogen content, phenol content. According to NH 4 + -N、NO 3 - The removal effect of N, total nitrogen and phenol content indexes is carried out, and 1 strain denitrified by phenol is obtained through re-screening, and is named TN-3.
The strain is spherical, red, round, 3-4mm in diameter, gram positive, iso-oxygen and aerobic.
The culture conditions of the strain are: 0.1mL of bacterial liquid is evenly coated on a denitrification culture medium (0.030 g/L phenol, 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride, 1.530 g/L potassium nitrate, 3.6g/L glucose, 0.2g/L potassium dihydrogen phosphate, 0.2g/L magnesium sulfate heptahydrate, 2g calcium carbonate and 1mL/L trace element solution (80 mg zinc chloride, 20mg anhydrous copper sulfate, 20mg boric acid, 100mg ferrous sulfate heptahydrate) and 1000mL water, 7-7.5), and the culture medium is placed in a constant temperature incubator for culture at 25 ℃ for 24h.
The application of the strain in degrading phenolic compounds in pesticide wastewater.
The strain is applied to denitrification of pesticide wastewater.
The application of the strain in degrading phenolic compounds and denitrifying in pesticide wastewater.
Culturing strain of Rhodococcus (Rhodococcus) TN-3 comprises activating, transferring, and amplifying,
activating: selecting single colony of dephenolization denitrifying bacteria from a solid plate, transferring the single colony into an LB liquid culture medium, and carrying out shake culture for 3-4 hours at 35-38 ℃ at the rotation speed of 130-180rpm until the logarithmic phase;
and (3) switching: transferring the dephenolization and denitrification bacterial liquid in the logarithmic phase into a seed tank for culture, controlling the temperature of the seed tank to be 25-30 ℃, controlling the rotating speed to be 220-250rpm, controlling the dissolved oxygen DO to be 2.5-3mg/L, and culturing for 24-48 h;
and (3) expanding cultivation: transferring the phenol denitrifying bacteria liquid cultured in the seed tank into a fermentation tank according to the inoculation amount of 2% -5%, and performing expansion culture, wherein the culture medium components of the fermentation tank are the same as those of the seed tank, and the physical and chemical parameter indexes are as follows: the temperature is 25-30 ℃, the rotating speed is 220-250rpm, the dissolved oxygen is 2.5-3mg/L, and the fermentation time is 48-60 h.
The LB liquid culture medium comprises the following components: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, and pH value maintained between 7.2-7.5.
The culture medium of the seed tank comprises the following components: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, potassium nitrate 3g/L, glucose 3.6g/L, potassium dihydrogen phosphate 0.2g/L, magnesium sulfate heptahydrate 0.2g/L, calcium carbonate 2g, trace element solution 1mL/L (zinc chloride 80mg, anhydrous copper sulfate 20mg, boric acid 20mg, ferrous sulfate heptahydrate 100 mg), water 1000mL, pH7-7.5.
The effective viable count of the bacterial liquid of the strain after the strain is cultured can reach 10 9 And (3) packaging the fermentation culture solution after the fermentation culture solution is taken out of the tank to obtain the high-efficiency phenol denitrifying bacteria agent.
The strain can strengthen the processing effect of the facultative FMBR pesticide wastewater processing technology, and the strain TN-3 screened in a laboratory for efficiently degrading phenolic organic pollutants in pesticide wastewater and denitrifying is added into a facultative FMBR processing system to form an efficient composite flora mainly containing facultative anaerobes (up to 80%), so that the phenol and nitrogen removal efficiency is greatly improved.
The strain TN-3 can grow in the waste water containing benzene rings and phenolic compounds below 0.5g/L, and the multiplication period is 36h.
The application of the high-efficiency dephenolization denitrifying bacteria in facultative FMBR treatment comprises the following steps: and (3) adjusting the pH and the pollutant concentration of the wastewater and the concentration of dissolved oxygen. Firstly, pH and pollutant concentration adjustment and dissolved oxygen concentration adjustment are carried out before water inflow according to the characteristics of pesticide wastewater, so that the water inflow load meets the requirement of microbial degradation (pH is 6-9, and the dissolved oxygen amount is 2.5-3 mg/L). And secondly, using a bacterial strain TN-3 screened in a laboratory for efficiently degrading and denitrifying phenolic organic pollutants in pesticide wastewater, and performing a process for degrading and denitrifying the phenolic pollutants under the condition that dissolved oxygen is controlled to be more than 2.5 mg/L.
The COD concentration of the pesticide wastewater is 2500mg/L-3500mg/L, the concentration of phenolic organic matters is 250mg/L-350mg/L, and the pH value is 6-9.
The addition amount of the microorganism TN-3 is 1% -10%.
The efficient degradation of phenolic organic pollutants in pesticide wastewater and denitrification bacterial strain used in the microbial denitrification process is TN-3 (Rhodococcus) and the laboratory screens and stores the high-phenolic and high-nitrogen pollutants in the common microorganism center of China Commission on culture Collection of microorganisms (CGMCC) with the preservation number of 23936 from the sediment of a river in a salt city. The microorganism is amplified by using a common culture medium, and the inoculation concentration is 5%. The residence time in the microorganism facultative FMBR process is 48h-36h.
The reaction mechanism of the process of the invention is as follows:
1. the COD mechanism of microbial degradation:
the biological metabolism pesticide waste water is utilized to contain a large amount of complex macromolecular organic substances such as phenol, and the substances are difficult to be degraded by microorganisms directly under the aerobic condition. The anaerobic or facultative anaerobic process can change the chemical structure by a large number of facultative anaerobic bacteria, so that the biodegradability of the anaerobic or facultative anaerobic bacteria is improved, and the screened strain TN-3 is utilized at the stage, so that the COD removal effect of the wastewater is sometimes not obvious, but even is improved, most of complex macromolecular organic matters of the wastewater can be converted into simple and easily degradable organic matters, the phenol ring is opened, the condition is provided for the subsequent aerobic biological treatment process, and the burden is reduced.
2. Microbial degradation ammonia nitrogen mechanism:
organic matters and NO in the microenvironment of the sludge and the surface inside thereof 3 - 、NO 2 - And the transfer of dissolved oxygen and the like is changed, and the transfer of the dissolved oxygen from the surface of the sludge floc to the inside is blocked, so that the dissolved oxygen value has a certain gradient. The surface of the sludge has relatively high concentration of dissolved oxygen, most of the dissolved oxygen is aerobic microorganisms such as nitrifying bacteria, oxygen is consumed when autotrophic nitrifying bacteria perform nitrifying, and the dissolved oxygen is blocked in the transfer process, so that local anoxic and anaerobic environments are easily caused in the sludge, denitrifying bacteria are dominant, and a large amount of denitrifying is performed. The consumption of ammonia nitrogen is increased, namely the ammonia nitrogen removal efficiency is increased.
3. Microbial color reduction mechanism:
under the facultative environmental condition of the facultative FMBR technology, the facultative anaerobic microorganisms decompose colored complex substances into simple micromolecular substances by utilizing the action of extracellular enzymes, and then the simple micromolecular substances are further reduced into simple compounds by certain acidizing bacteria, at the moment, aromatic substances are also degraded into organic acids, and simultaneously, chromophores of the colored complex substances are also destroyed, so that the chromaticity of the pesticide wastewater is removed mostly in a facultative region of the device in the experiment.
4. Membrane adsorption mechanism:
membrane removal of organics in wastewater includes three reasons: firstly, utilizing the mechanical screening action of the membrane hole; secondly, utilizing the adsorption effect of the membrane surface and the membrane holes on organic matters; and finally, utilizing the adsorption of a sludge deposition layer on the surface of the membrane and mechanical screening. It should be noted that these three reasons are not the same in importance for membrane removal of organics in wastewater, the first reason being mechanical screening, which only entraps larger organics in wastewater relative to the membrane pores, while other relatively smaller organics are removed primarily for the latter two reasons. With the continuous operation of the system, the membrane holes are inevitably blocked to different degrees, at the moment, deposited sludge on the surface of the membrane reduces the membrane holes, the entrapment efficiency of the membrane is improved, the third reason is similar to the principle of a biological membrane method, and the later period in the operation of the system is the most important reason for removing organic matters by the membrane.
The beneficial effects are that:
1. the dephenolization denitrifying bacteria TN-3 uses phenolic pollutants as a nutrient source to carry out denitrification reaction, so that the cost of carbon sources is saved, and the efficiency of the facultative FMBR system is improved.
2. Because the membrane has super-strong solid-liquid separation capability, facultative FMBR can retain most microorganisms so as to maintain higher concentration in the reactor, the sludge load is greatly reduced, and the impact load resistance capability is strong.
3. The facultative FMBR technology prolongs the sludge age, and the reactor plays a role of a sludge nitrification tank in the traditional technology, so that the sludge yield is greatly reduced, and the sludge treatment cost is reduced. Because the facultative FMBR technology has longer sludge age, nitrifying and denitrifying bacteria with long generation time can be trapped, the denitrification capability of wastewater is improved, and the degradation capability of macromolecular organic substances is improved.
Detailed Description
The pesticide wastewater in the experiment is derived from the production wastewater of some farm in Jiangsu salt city, and the main pollutants are nitrogen-containing heterocycle and phenolic compounds. The added activated sludge is the residual sludge of a secondary sedimentation tank in the sewage treatment facility of the factory. The obtained sludge is placed in a refrigerator with the temperature of 4 ℃ for preservation, taken out when the sludge is needed to be used, activated by LB culture medium and then subjected to experiments.
The COD measurement method in the following examples is: potassium dichromate process; the method for measuring total nitrogen comprises the following steps: ultraviolet spectrophotometry.
Example 1 screening of strains
The strain is obtained by screening from the sediment of a river in a salt city, and the specific screening method is as follows:
50mL of river mud from a river in the salt city was added using 0.300g/L of phenol solution and 1g of sodium chloride as a screening medium. After 30d, the phenol degraded to 0.015g/L. Inoculating the supernatant to a denitrification medium (1.530 g/L potassium nitrate, 3.6g/L glucose, 0.2g/L potassium dihydrogen phosphate, 0.2g/L magnesium sulfate heptahydrate, 2g calcium carbonate, 1mL/L microelement solution (chlorine)80mg of zinc oxide, 20mg of anhydrous copper sulfate, 20mg of boric acid, 100mg of ferrous sulfate heptahydrate), 1000mL of water and pH 7-7.5), and measuring NH every 12h 4 + -N、NO 3 - -N, total nitrogen content. According to NH 4 + -N、NO 3 - And (3) removing the N and total nitrogen indexes, and re-screening to obtain 1 strain denitrified by phenol, namely TN-3.
The strain is rod-shaped, has a capsule, has no spore, is gram-negative, is iso-oxygen, is strictly aerobic and is positive in a nitrate reduction test.
EXAMPLE 2 cultivation of strains
The strain culture comprises the steps of activation, transfer and expansion culture,
activating: single colony of dephenolization denitrifying bacteria is selected from the solid flat plate and transferred into LB liquid medium, the rotation speed of a shaking table is 150rpm, and shaking table culture is carried out for 3-4 hours at 35 ℃ until the logarithmic phase;
and (3) switching: transferring the dephenolization and denitrification bacterial liquid in the logarithmic phase into a seed tank for culture, controlling the temperature of the seed tank to be 28 ℃, controlling the rotating speed to be 250rpm, controlling the dissolved oxygen DO to be 2.5-3mg/L, and culturing for 24 hours;
and (3) expanding cultivation: transferring the phenol denitrifying bacteria liquid cultured in the seed tank into a fermentation tank according to the inoculation amount of 2% -5%, and performing expansion culture, wherein the culture medium components of the fermentation tank are the same as those of the seed tank, and the physical and chemical parameter indexes are as follows: the temperature is 28 ℃, the rotating speed is 250rpm, the dissolved oxygen is 2.5-3mg/L, and the fermentation time is 48 hours.
The LB liquid culture medium comprises the following components: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, and pH value maintained between 7.2-7.5.
The culture medium of the seed tank comprises the following components: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, potassium nitrate 3g/L, glucose 3.6g/L, potassium dihydrogen phosphate 0.2g/L, magnesium sulfate heptahydrate 0.2g/L, calcium carbonate 2g, trace element solution 1mL/L (zinc chloride 80mg, anhydrous copper sulfate 20mg, boric acid 20mg, ferrous sulfate heptahydrate 100 mg), water 1000mL, pH7-7.5.
The effective viable count of the bacterial liquid of the strain after the strain is cultured can reach 10 9 And (3) packaging the fermentation culture solution after the fermentation culture solution is taken out of the tank to obtain the high-efficiency phenol denitrifying bacteria agent.
Example 3
The bacterial liquid in example 2 was uniformly coated on a denitrification medium (0.300 g/L phenol, 3g/L beef extract, 10g/L peptone, 5g/L sodium chloride, 1.530 g/L potassium nitrate, 3.6g/L glucose, 0.2g/L potassium dihydrogen phosphate, 0.2g/L magnesium sulfate heptahydrate, 2g calcium carbonate, 1mL/L trace element solution (80 mg zinc chloride, 20mg anhydrous copper sulfate, 20mg boric acid, 100mg ferrous sulfate heptahydrate, 1000mL water, pH 7-7.5), and the medium was cultured in a constant temperature incubator at 25℃for 24 hours until the phenol was degraded to 0.010g/L and the phenol degradation rate reached 97%.
Example 4:
the pH value of the pesticide wastewater is regulated to 7 by using 20% liquid alkali, the pesticide wastewater is diluted by low-concentration sewage in a factory, and COD of the inflow water is regulated to 3g/L, wherein the total nitrogen of phenol is 0.300g/L and the total nitrogen is 0.300g/L. Adding the regulated sewage into a facultative FMBR system, keeping dissolved oxygen at 2.5-3mg/L, using common activated sludge of an agricultural chemical factory to carry out microorganism, adding a dephenolization denitrification bacterial liquid TN-3, and carrying out dephenolization denitrification reaction.
COD of wastewater inflow: 3g/L;
waste water inlet phenol content: 0.300g/L;
total nitrogen in wastewater: 0.300g/L;
the adding amount of the dephenolization denitrifying bacteria TN-3: 5%;
microbial dephenolization denitrification residence time: 24h;
COD after dephenolization and denitrification of wastewater: 0.650g/L;
phenol content of wastewater dephenolization and denitrification: 0.080g/L;
total nitrogen after dephenolization and denitrification of wastewater: 0.075g/L.
Example 5:
the pH value of the pesticide wastewater is regulated to 7 by using 20% liquid alkali, the pesticide wastewater is diluted by low-concentration sewage in a factory, and COD of the inflow water is regulated to 3g/L, wherein the total nitrogen of phenol is 0.300g/L and the total nitrogen is 0.300g/L. Adding the regulated sewage into a facultative FMBR system, keeping dissolved oxygen at 2.5-3mg/L, using common activated sludge of an agricultural chemical factory to carry out microorganism, adding a dephenolization denitrification bacterial liquid TN-3, and carrying out dephenolization denitrification reaction.
COD of wastewater inflow: 3g/L;
waste water inlet phenol content: 0.300g/L;
total nitrogen in wastewater: 0.300g/L;
the adding amount of the dephenolization denitrifying bacteria TN-3: 5%;
microbial dephenolization denitrification residence time: 48h;
COD after dephenolization and denitrification of wastewater: 0.250g/L;
phenol content of wastewater dephenolization and denitrification: 0.040g/L;
total nitrogen after dephenolization and denitrification of wastewater: 0.045g/L.
Comparative example 1:
the pH value of the pesticide wastewater is regulated to 7 by using 20% liquid alkali, the pesticide wastewater is diluted by low-concentration sewage in a factory, and COD of the inflow water is regulated to 3g/L, wherein the total nitrogen of phenol is 0.300g/L and the total nitrogen is 0.300g/L. Adding the regulated sewage into a biochemical pond instead of a facultative FMBR system, keeping dissolved oxygen at 2.5-3mg/L, and performing microbial dephenolization denitrification reaction by using common activated sludge of an agricultural chemical factory.
COD content of wastewater: 3g/L;
waste water inlet phenol content: 0.300g/L;
total nitrogen in wastewater: 0.300g/L;
microbial dephenolization denitrification residence time: 48h;
COD after dephenolization and denitrification of wastewater: 2.1g/L;
phenol content of wastewater dephenolization and denitrification: 0.250g/L;
total nitrogen after dephenolization and denitrification of wastewater: 0.230g/L.
Comparative example 2
The pH value of the pesticide wastewater is regulated to 7 by using 20% liquid alkali, the pesticide wastewater is diluted by low-concentration sewage in a factory, and COD of the inflow water is regulated to 3g/L, wherein the total nitrogen of phenol is 0.300g/L and the total nitrogen is 0.300g/L. Adding the regulated sewage into a facultative FMBR system, keeping dissolved oxygen at 2.5-3mg/L, and using common activated sludge of an agricultural chemical factory to carry out a microorganism dephenolization denitrification reaction.
COD of wastewater inflow: 3g/L;
waste water inlet phenol content: 0.300g/L;
total nitrogen in wastewater: 0.300g/L;
microbial dephenolization denitrification residence time: 48h;
COD after dephenolization and denitrification of wastewater: 1.6g/L;
phenol content of wastewater dephenolization and denitrification: 0.200g/L;
total nitrogen after dephenolization and denitrification of wastewater: 0.180g/L.
Therefore, in the traditional FMBR, the strain TN-3 for efficiently degrading phenolic organic pollutants in pesticide wastewater and denitrifying the same is added, so that the phenolic organic pollutants and total nitrogen in the wastewater can be effectively removed, the water treatment cost is reduced, the water treatment efficiency is improved, the process treatment effect of facultative FMBR for pesticide wastewater is enhanced, and the phenol removal and denitrification efficiency is greatly improved.
Claims (10)
1. Degrading phenolic organic pollutants in pesticide wastewater and denitrifying bacteria, wherein the classification of the denitrifying bacteria is named as rhodococcus biennis @Rhodococcus electrodiphilus) TN-3 is preserved in China general microbiological culture collection center with a preservation number of CGMCC No.23936.
2. The use of the denitrifying bacteria for degrading phenolic organic pollutants in pesticide wastewater according to claim 1 in wastewater denitrification.
3. The use of the denitrifying bacteria for degrading phenolic organic pollutants in pesticide wastewater according to claim 1 in degrading pesticide phenol-containing wastewater.
4. A use according to claim 2 or 3, characterized in that: the rhodococcus bifidus is treatedRhodococcus electrodiphilus) After the TN-3 is cultured, the TN-3 is added into the wastewater to be treated to denitrify or degrade phenolic organic matters.
5. The use according to claim 4, characterized in that: rhodococcus bifidus @Rhodococcus electrodiphilus) TN-3 is used for culturing strains, which comprises the steps of activation, transfer and expansion culture,
activating: single colony of dephenolization denitrifying bacteria is selected from the solid plate and transferred into LB liquid culture medium, the rotation speed of a shaking table is 130-180rpm, and shaking table culture is carried out at 35-38 ℃ for 3-4h until the logarithmic phase;
and (3) switching: transferring the dephenolization and denitrification bacterial liquid in the logarithmic phase into a seed tank for culture, controlling the temperature of the seed tank to be 25-30 ℃, controlling the rotating speed to be 220-250rpm, controlling the dissolved oxygen DO to be 2.5-3mg/L, and culturing for 24-48 h;
and (3) expanding cultivation: transferring the phenol denitrifying bacteria liquid cultured in the seed tank into a fermentation tank according to the inoculation amount of 2% -5%, and performing expansion culture, wherein the culture medium components of the fermentation tank are the same as those of the seed tank, and the physical and chemical parameter indexes are as follows: the temperature is 25-30 ℃, the rotating speed is 220-250rpm, the dissolved oxygen is 2.5-3mg/L, and the fermentation time is 48-60 h.
6. The use according to claim 5, characterized in that: the LB liquid culture medium comprises the following components: beef extract 3g/L, peptone 10g/L, sodium chloride 5g/L, and pH value maintained between 7.2-7.5.
7. The use according to claim 5, characterized in that: the culture medium of the seed tank comprises the following components: 3g/L of beef extract, 10g/L of peptone, 5g/L of sodium chloride, 3g/L of potassium nitrate, 3.6g/L of glucose, 0.2g/L of monopotassium phosphate, 0.2g/L of magnesium sulfate heptahydrate, 2g of calcium carbonate, 1mL/L of microelement solution, 1000mL of water and pH7-7.5.
8. The use according to claim 5, characterized in that: the effective viable count of the bacterial liquid after the bacterial strain is cultured is 10 9 The microbial inoculum can be obtained by packaging the fermentation culture solution after the fermentation culture solution is taken out of the tank.
9. The use according to claim 7, characterized in that: each milliliter of the trace element solution contains 80mg of zinc chloride, 20mg of anhydrous copper sulfate, 20mg of boric acid and 100mg of ferrous sulfate heptahydrate.
10. A use according to claim 3, characterized in that: the concentration of phenol in the pesticide phenol-containing wastewater is 250mg/L-350mg/L.
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