CN114772715B - Application of streptomycete and bacillus in promoting formation of aerobic granular sludge - Google Patents
Application of streptomycete and bacillus in promoting formation of aerobic granular sludge Download PDFInfo
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- 239000010802 sludge Substances 0.000 title claims abstract description 134
- 241000193830 Bacillus <bacterium> Species 0.000 title claims abstract description 70
- 241001655322 Streptomycetales Species 0.000 title claims abstract description 33
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 21
- 230000001737 promoting effect Effects 0.000 title claims abstract description 19
- 238000012258 culturing Methods 0.000 claims abstract description 64
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 41
- 241000187747 Streptomyces Species 0.000 claims abstract description 37
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims description 43
- 230000001580 bacterial effect Effects 0.000 claims description 37
- 238000004321 preservation Methods 0.000 claims description 25
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 abstract description 7
- 239000001963 growth medium Substances 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 239000012880 LB liquid culture medium Substances 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 2
- 241000228143 Penicillium Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000010333 potassium nitrate Nutrition 0.000 description 2
- 239000004323 potassium nitrate Substances 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000012137 tryptone Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 241000985513 Penicillium oxalicum Species 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/44—Time
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Treatment Of Sludge (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses application of streptomyces and bacillus in promoting formation of aerobic granular sludge. According to the invention, the streptomycete and the bacillus are respectively mixed after being subjected to expansion culture to prepare the functional microbial inoculum, and then the aerobic granular sludge is cultured by utilizing the functional microbial inoculum, so that the aerobic granular sludge can be obtained after culturing for 4-14 days, the time for culturing the aerobic granular sludge can be shortened, the formed aerobic granular sludge has smooth surface and compact structure, the functional microbial inoculum can effectively promote the formation of the aerobic granular sludge, the formed aerobic granular sludge has good stability, and the method is suitable for rapidly culturing the aerobic granular sludge and quickens the starting speed of a membrane bioreactor.
Description
Technical Field
The invention belongs to the technical field of biological wastewater treatment. More particularly, to the use of streptomyces and bacillus to promote the formation of aerobic granular sludge.
Background
The use of activated sludge to treat and purify wastewater is currently one of the most widely used methods in the field of wastewater treatment. Wherein, the Aerobic Granular Sludge (AGS) is granular activated sludge formed by self-agglomeration of microorganisms under aerobic conditions. Compared with the traditional activated sludge method, the aerobic granular sludge has the advantages of low total energy consumption, low civil engineering cost and good effluent quality. Compared with flocculent activated sludge, the aerobic granular sludge has high microorganism content, strong activity and good sedimentation performance. Compared with anaerobic granular sludge, the method has the advantages of short forming period, low severity of culture conditions and wide application range of aerobic granular sludge. Therefore, the aerobic granular sludge technology has been studied for a large number of urban sewage treatment technologies in recent years. However, the application of the aerobic granular sludge technology is greatly limited at present due to the slow granulating speed and poor stability of the aerobic granular sludge. Therefore, the product or the method for promoting the formation of the aerobic granular sludge is of great significance in promoting the development of the aerobic granular sludge technology, improving the efficiency and the quality of wastewater treatment and the like.
Currently, methods for promoting the formation of aerobic granular sludge include improvement of process equipment, optimization of culture methods, addition of specific bacterial agents to activated sludge, and the like. For example, chinese patent CN113402018A discloses a method for fast culturing aerobic granular sludge by using penicillium oxalate, which comprises adding penicillium oxalate granular bacterial liquid into a reactor, and culturing for 12-16 days to obtain aerobic granular sludge, wherein the formed granular sludge is regular and compact, and has good sedimentation property. Although the cultivation time of the aerobic granular sludge can be reduced by using the penicillium oxalicum, the cultivation period is relatively long, and the cultivation time is still further improved.
Disclosure of Invention
The invention aims to overcome the defects and the shortcomings of the prior art and provide the application of streptomycete and bacillus in promoting the formation of aerobic granular sludge.
The first object of the present invention is to provide the use of Streptomyces and Bacillus for promoting the formation of aerobic granular sludge.
A second object of the invention is to improve the use of Streptomyces and Bacillus for the preparation of a formulation for promoting the formation of aerobic granular sludge.
The third object of the invention is to provide a functional microbial inoculum for promoting the formation of aerobic granular sludge.
The fourth object of the invention is to provide a method for promoting the formation of aerobic granular sludge by using streptomyces and bacillus.
The above object of the present invention is achieved by the following technical scheme:
in the early stage of the invention, a series of experiments show that the combination of streptomycete and bacillus can promote the formation of aerobic granular sludge. On the basis, the streptomycete and the bacillus are respectively mixed after being subjected to expansion culture to prepare the functional microbial inoculum, and the aerobic granular sludge is cultured by utilizing the functional microbial inoculum, so that the time for culturing the aerobic granular sludge can be shortened, and the formed aerobic granular sludge has smooth surface and compact structure, and shows that the formed aerobic granular sludge has good stability.
Therefore, the invention discloses the application of streptomyces and bacillus in promoting the formation of aerobic granular sludge.
Specifically, the preservation number of the streptomyces is CICC 11030, and the preservation number of the bacillus is CICC 10142.
The invention also discloses application of streptomyces and bacillus in preparation of a preparation for promoting formation of aerobic granular sludge.
Specifically, the preservation number of the streptomyces is CICC 11030, and the preservation number of the bacillus is CICC 10142.
The invention also provides a functional microbial inoculum for promoting the formation of aerobic granular sludge, wherein the functional microbial inoculum comprises streptomycete and bacillus.
Specifically, the preservation number of the streptomyces is CICC 11030, and the preservation number of the bacillus is CICC 10142.
Specifically, the volume ratio of streptomycete to bacillus in the functional microbial inoculum is 10:0 to 10.
Preferably, the volume ratio of streptomycete to bacillus in the functional microbial inoculum is 10:2.5 to 5, see example 4.
Specifically, the colony concentration of streptomycete in the functional microbial inoculum is 10 6 ～10 8 CFU/mL, colony concentration of Bacillus was 10 8 ～10 10 CFU/mL。
The invention also provides a method for promoting the formation of aerobic granular sludge by using streptomycete and bacillus, which comprises the following steps:
s1, culturing streptomycete; culturing under aseptic condition to obtain colony concentration of 10 6 ～10 8 CFU/mL of streptomycete bacterial liquid;
s2, culturing bacillus; culturing under aseptic condition to obtain colony concentration of 10 8 ～10 10 CFU/mL bacillus bacterial liquid;
s3, culturing aerobic granular sludge; mixing the streptomyces bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained in the step S2 by 10: mixing uniformly in a volume ratio of 0-10 to prepare a functional microbial inoculum, and adding the obtained functional microbial inoculum into activated sludge for culture to obtain the aerobic granular sludge.
Specifically, in the step S3, if the aerobic granular sludge is cultured by shaking the container containing the activated sludge, the culture condition is 15-35 ℃ and 50-250 rpm; if the membrane bioreactor containing the activated sludge is used for culture, the initial concentration of the suspended solid concentration of the mixed solution in the reactor is 2000-4000 mg/L, the aerobic zone DO is 1.0-3.0 mg/L, and the anaerobic zone DO is 0-1.0 mg/L.
Specifically, the volume ratio of the functional microbial inoculum to the activated sludge added in the step S3 is 40:1 to 20.
Specifically, the preservation number of the streptomyces is CICC 11030, and the preservation number of the bacillus is CICC 10142.
Specifically, the culture medium used for culturing the streptomyces in the step S1 is a Gao' S first culture medium, the culture condition is 20-40 ℃, the culture speed is 0-300 rpm, and the culture time is 1-5 d.
Specifically, each 1L of culture medium of Gao's No. 1 contains 20g of soluble starch, 1g of potassium nitrate, 0.5g of sodium chloride, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate, 0.01g of ferrous sulfate and pH of 7.2-7.4.
Specifically, the culture medium used for culturing the bacillus in the step S2 is LB liquid culture medium, the culture condition is 20-40 ℃, the culture speed is 0-300 rpm, and the culture time is 1-5 d.
Specifically, 10g of tryptone, 5g of yeast extract, 10g of sodium chloride and pH of 7.0 were contained in each 1L of LB liquid medium.
Specifically, in the step S3, the volume ratio of the activated sludge to the streptomycete particle bacterial liquid to the bacillus bacterial liquid is 40:8:4, the effect of forming granular sludge is optimal.
The invention has the following beneficial effects:
according to the invention, the streptomycete and the bacillus are respectively mixed after being subjected to expansion culture to prepare the functional microbial inoculum, and then the aerobic granular sludge is cultured by utilizing the functional microbial inoculum, so that the aerobic granular sludge can be obtained after culturing for 4-14 days, the time for culturing the aerobic granular sludge can be shortened, the formed aerobic granular sludge has smooth surface and compact structure, the functional microbial inoculum can effectively promote the formation of the aerobic granular sludge, the formed aerobic granular sludge has good stability, and the method is suitable for rapidly culturing the aerobic granular sludge and quickens the starting speed of a membrane bioreactor.
The invention provides a new product and a method for the rapid culture of the aerobic granular sludge, which are beneficial to the rapid culture and application of the aerobic granular sludge.
Drawings
FIG. 1 is a morphology diagram of the aerobic granular sludge prepared in example 4.
FIG. 2 is a morphology graph of the aerobic granular sludge prepared in example 5.
FIG. 3 is a graph showing the morphology of the aerobic granular sludge produced in example 6.
Fig. 4 is an SEM image of the aerobic granular sludge prepared in example 6.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Reagents and materials used in the following examples are commercially available unless otherwise specified.
Example 1 cultivation of aerobic granular sludge in a shaker Using Streptomyces-Bacillus functional microbial agent
In the embodiment, the triangular flask containing the activated sludge is used for culturing the aerobic granular sludge in a shaking table, and the used bacteria are streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 10142.
S1, culturing streptomycete: inoculating Streptomyces to culture medium of Gao's No. 1 under aseptic condition, and culturing in liquid amplification for 4d at 37deg.C and 250rpm in shaking table to obtain colony concentration of 1×10 7 CFU/mL streptomycete granular bacterial liquid;
every 1L of culture medium with Gaoshi No. 1 contains 20g of soluble starch, 1g of potassium nitrate, 0.5g of sodium chloride, 0.5g of dipotassium hydrogen phosphate, 0.5g of magnesium sulfate and 0.01g of ferrous sulfate, and the pH value is 7.2-7.4.
S2, culturing bacillus: inoculating Bacillus to LB liquid culture medium under aseptic condition, placing in shaking table, and liquid-culturing at 37deg.C and 250rpm for 2d to obtain colony concentration of 3×10 9 CFU/mL bacillus bacterial liquid;
each 1L of LB liquid medium contains 10g of tryptone, 5g of yeast extract, 10g of sodium chloride and has a pH of 7.0.
S3, culturing aerobic granular sludge: mixing the streptomyces granular bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained by culturing in the step S2 by 8:2, uniformly mixing the mixture in a volume ratio to prepare a functional microbial inoculum, and immediately adding the functional microbial inoculum into a triangular flask containing activated sludge for shake culture; wherein, the volume ratio of the activated sludge to the streptomycete-bacillus functional microbial inoculum is 40:10, the culture temperature in the shaking table is 37 ℃ and the rotating speed is 200rpm, and the aerobic granular sludge with lighter color and regular shape can be obtained on the 10 th day of culture.
In this example, activated sludge without microbial inoculum was cultured under the same conditions, and aerobic granular sludge started to appear on day 16 of the culture. Therefore, the addition of the streptomycete-bacillus functional microbial inoculum has a promoting effect on the formation of aerobic granular sludge.
Example 2 cultivation of aerobic granular sludge in a shaker Using different Streptomyces-Bacillus functional inoculants
This example differs from example 1 in that the Streptomyces and Bacillus are used. The present example used the same method as in example 1, and used Streptomyces deposited with accession number CICC 11040 and Bacillus deposited with accession number CICC 21298, streptomyces deposited with accession number CICC 11030 and Bacillus deposited with accession number CICC 21290, and Streptomyces deposited with accession number CICC 11056 and Bacillus deposited with accession number CICC 21290 to prepare functional microbial inoculum of Streptomyces-Bacillus, and then the prepared functional microbial inoculum was added into a triangular flask containing activated sludge, and subjected to shaking culture to obtain aerobic granular sludge.
The result shows that the functional microbial inoculum prepared by adding streptomycete with the preservation number of CICC 11040 and bacillus with the preservation number of CICC 21298 can obtain the aerobic granular sludge with lighter color and regular shape on the 14 th day of culture; adding a functional microbial inoculum prepared from streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 21290, and obtaining the aerobic granular sludge with lighter color and regular shape on the 12 th day of culture; adding functional bacteria agent prepared from Streptomyces with preservation number of CICC 11056 and bacillus with preservation number of CICC 21290, and culturing for 14 days to obtain the aerobic granular sludge with lighter color and regular shape.
Example 3 cultivation of aerobic granular sludge in a shaker Using Streptomyces-Bacillus functional microbial agent
In the embodiment, the triangular flask containing the activated sludge is used for culturing the aerobic granular sludge in a shaking table, and the used bacteria are streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 10142.
S1, culturing streptomycete: inoculating Streptomyces to culture medium of Gao's No. 1 under aseptic condition, and culturing in liquid amplification at 25deg.C and 150rpm in shaking table for 2d to obtain colony concentration of 2×10 6 CFU/mL streptomycete granular bacterial liquid;
s2, culturing bacillus: inoculating Bacillus to LB liquid culture medium under aseptic condition, placing in shaking table, and liquid culturing at 25deg.C and 150rpm for 3d to obtain colony concentration of 4×10 9 CFU/mL bacillus bacterial liquid;
s3, culturing aerobic granular sludge: mixing the streptomyces granular bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained by culturing in the step S2 by 8:4, mixing uniformly to prepare a functional microbial inoculum, immediately adding the functional microbial inoculum into a triangular flask containing activated sludge for shake culture, wherein the volume ratio of the activated sludge to the streptomycete-bacillus functional microbial inoculum is 40:12, the culture temperature in the shaking table is 37 ℃ and the rotating speed is 150rpm, and the aerobic granular sludge with lighter color and regular shape can be obtained on the 6 th day of culture.
Example 4 cultivation of aerobic granular sludge with Streptomyces-Bacillus functional inoculants in different ratios
In the embodiment, the triangular flask containing the activated sludge is used for culturing the aerobic granular sludge in a shaking table, and the used bacteria are streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 10142.
S1, culturing streptomycete: inoculating Streptomyces to culture medium of Gao's No. 1 under aseptic condition, and culturing in liquid amplification at 30deg.C and 200rpm in shaking table for 5 days to obtain colony concentration of 8X10 7 CFU/mL streptomycete granular bacterial liquid;
s2, culturing bacillus: inoculating Bacillus to LB liquid culture medium under aseptic condition, placing in shaking table, and liquid-culturing at 30deg.C and 200rpm for 5d to obtain colony concentration of 7X10 9 CFU/mL bacillus bacterial liquid;
s3, culturing aerobic granular sludge: the streptomycete particle bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained by culturing in the step S2 are respectively mixed with 0:5 and 10: 0. 2.5, 5, 7.5, 10, 15 and 20, and respectively adding the functional microbial inoculum into a triangular flask containing the activated sludge for shake culture, wherein the volume ratio of the activated sludge to the functional microbial inoculum is 40:10, the culture temperature in the shaking table is 30 ℃, the rotating speed is 200rpm, and the time for forming the aerobic granular sludge by the functional microbial inoculum with different proportions is recorded. The results are shown in Table 1:
TABLE 1 promotion effect of Streptomyces-Bacillus in different proportions on aerobic granular sludge
The morphology diagram of the aerobic granular sludge obtained by culturing in the embodiment is shown in fig. 1, and the volume ratio of streptomycete to bacillus is 10:15 and 10: at 20, aerobic granular sludge cannot be formed, and the volume ratio of the result to streptomycete and bacillus is 0:5, the same applies, so there is no figure. As can be seen from the results shown in table 1 and fig. 1, the volume ratio of the streptomycete particle bacterial liquid to the bacillus bacterial liquid is 10:2.5 or 10: and 5, the promoting effect on forming the aerobic granular sludge is optimal.
Example 5 cultivation of aerobic granular sludge with different proportions of activated sludge and functional microbial agent
In the embodiment, the triangular flask containing the activated sludge is used for culturing the aerobic granular sludge in a shaking table, and the used bacteria are streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 10142.
S1, culturing streptomycete: inoculating Streptomyces to culture medium of Gao's No. 1 under aseptic condition, and culturing in liquid amplification at 30deg.C and 200rpm for 1d to obtain colony concentration of 1×10 6 CFU/mL streptomycete granular bacterial liquid;
s2, culturing bacillus: inoculating Bacillus to LB liquid culture medium under aseptic condition, placing in shaking table, and liquid-culturing at 30deg.C and 200rpm for 2d to obtain colony concentration of 3×10 9 CFU/mL bacillus bacterial liquid;
s3, culturing aerobic granular sludge: mixing the streptomyces granular bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained by culturing in the step S2 by 2:1, respectively adding the functional microbial inoculum into triangular flasks containing the activated sludge for shake culture, wherein the volume ratio of the activated sludge to the functional microbial inoculum is 40: 0. 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20, the culture temperature of a shaking table is 30 ℃, the rotation speed is 200rpm, the culture temperature in the shaking table is 30 ℃, the rotation speed is 200rpm, and the time for forming the aerobic granular sludge by the activated sludge and the functional microbial inoculum in different proportions is recorded. The results are shown in Table 2:
TABLE 2 promoting effect of activated sludge and functional microbial Agents on aerobic granular sludge in different proportions
The morphology of the aerobic granular sludge obtained by the culture in this example is shown in FIG. 2. As can be seen from the results shown in table 2 and fig. 2, the volume ratio of the activated sludge to the streptomycete granule bacterial liquid to the bacillus bacterial liquid is 40:8:4, the effect of the formed granular sludge is optimal.
Example 6 cultivation of aerobic granular sludge in an internal circulation Membrane bioreactor Using functional microbial Agents
In the embodiment, the aerobic granular sludge is cultivated by using an inner loop flow membrane bioreactor containing activated sludge, and the used bacteria are streptomyces with the preservation number of CICC 11030 and bacillus with the preservation number of CICC 10142.
S1, culturing streptomycete: inoculating Streptomyces to culture medium of Gao's No. 1 under aseptic condition, and culturing in liquid amplification at 30deg.C and 200rpm for 1d to obtain colony concentration of 1×10 6 CFU/mL streptomycete granular bacterial liquid;
s2, culturing bacillus: inoculating Bacillus to LB liquid culture medium under aseptic condition, placing in shaking table, and liquid-culturing at 30deg.C and 200rpm for 2d to obtain colony concentration of 3×10 9 CFU/mL bacillus bacterial liquid;
s3, culturing aerobic granular sludge: mixing the streptomyces granular bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained by culturing in the step S2 by 2:1, uniformly mixing the mixture in a volume ratio to prepare a functional microbial inoculum, and adding the functional microbial inoculum into an inner loop flow membrane bioreactor containing activated sludge to culture aerobic granular sludge, wherein the volume ratio of the activated sludge to the functional microbial inoculum is 40:12, the culture condition of the inner loop flow membrane bioreactor is that the initial concentration of MLSS is 2600mg/L, the DO of the aerobic zone is 2.0mg/L, the DO of the anaerobic zone is 1.0mg/L, and the aerobic granular sludge can be obtained on the 7 th day.
The morphology diagram of the aerobic granular sludge obtained by culture in the embodiment is shown in fig. 3, the SEM diagram is shown in fig. 4, and the obtained granular sludge has smooth surface and compact structure as shown in fig. 3 and 4, which indicates that the added functional microbial inoculum plays a role, can effectively promote the formation of the aerobic granular sludge, and the formed aerobic granular sludge has good stability.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (3)
1. A method for promoting the formation of aerobic granular sludge by using streptomyces and bacillus, which is characterized by comprising the following steps:
s1, culturing streptomycete; culturing under aseptic condition to obtain colony concentration of 10 6 ～10 8 CFU/mL of streptomycete bacterial liquid; the preservation number of the streptomyces is CICC 11030, CICC 11040 or CICC 11056;
s2, culturing bacillus; culturing under aseptic condition to obtain colony concentration of 10 8 ～10 10 CFU/mL bacillus bacterial liquid; the bacillus is deposited with the accession number of CICC 21298, CICC 21290 or CICC 10142;
s3, culturing aerobic granular sludge; mixing the streptomyces bacterial liquid obtained by culturing in the step S1 and the bacillus bacterial liquid obtained in the step S2 by 10:2.5 to 10, and evenly mixing the mixture to prepare a functional microbial inoculum, and adding the obtained functional microbial inoculum into activated sludge for culture to obtain aerobic granular sludge;
in the step S3, if the aerobic granular sludge is cultured by shaking the container containing the activated sludge, the culture condition is 15-35 ℃ and 50-250 rpm.
2. The method according to claim 1, wherein the volume ratio of the functional bacterial agent to the activated sludge added in step S3 is 40:1 to 20.
3. The method of claim 1, wherein the Streptomyces is deposited under accession number CICC 11030 and the Bacillus is deposited under accession number CICC 10142.
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| CN202210461650.0A CN114772715B (en) | 2022-04-28 | 2022-04-28 | Application of streptomycete and bacillus in promoting formation of aerobic granular sludge |
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| CN114772715B true CN114772715B (en) | 2023-11-03 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8718676D0 (en) * | 1986-08-06 | 1987-09-09 | Nippon Steel Corp | Treatment of sewage |
| CN101698534A (en) * | 2009-11-12 | 2010-04-28 | 上海交通大学 | Method for optimizing stability of aerobic granular sludge |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8718676D0 (en) * | 1986-08-06 | 1987-09-09 | Nippon Steel Corp | Treatment of sewage |
| CN101698534A (en) * | 2009-11-12 | 2010-04-28 | 上海交通大学 | Method for optimizing stability of aerobic granular sludge |
Non-Patent Citations (1)
| Title |
|---|
| 宋志伟等.微生物絮凝剂投加方式对好氧颗粒污泥性能的影响.吉林大学学报(地球科学版).2015,(第01期),第247-253页摘要、第1-3节. * |
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