CN114716007B - Application of streptomycete in promoting formation of aerobic granular sludge and/or improving stability of aerobic granular sludge - Google Patents
Application of streptomycete in promoting formation of aerobic granular sludge and/or improving stability of aerobic granular sludge Download PDFInfo
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- 239000010802 sludge Substances 0.000 title claims abstract description 205
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 21
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 241001655322 Streptomycetales Species 0.000 title claims description 64
- 238000012258 culturing Methods 0.000 claims abstract description 78
- 241000187747 Streptomyces Species 0.000 claims abstract description 68
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- 238000004321 preservation Methods 0.000 claims description 17
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- 239000007787 solid Substances 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 4
- 238000012136 culture method Methods 0.000 claims description 3
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- 239000011259 mixed solution Substances 0.000 description 6
- 241000233866 Fungi Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
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- 230000000052 comparative effect Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
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- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
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- 239000004323 potassium nitrate Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
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- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
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- 235000020679 tap water Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000222178 Candida tropicalis Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000228153 Penicillium citrinum Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
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- 241001446247 uncultured actinomycete Species 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/44—Time
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses application of streptomyces in promoting formation of aerobic granular sludge and/or improving stability of the aerobic granular sludge. The invention provides application of streptomyces with deposit numbers of CICC 10513, CICC11035, CICC11010 and/or CICC21933 in promoting formation of aerobic granular sludge and/or improving stability of the aerobic granular sludge, and provides a method for preparing the aerobic granular sludge by using the streptomyces. The method can be used for culturing and obtaining the aerobic granular sludge within 6-12 days, and the surface of the aerobic granular sludge obtained by culturing is smooth, regular in shape, compact in texture and good in stability, and the stability of the aerobic granular sludge can be improved while the formation time of the aerobic granular sludge is shortened.
Description
Technical Field
The invention belongs to the technical field of biological wastewater treatment. In particular to the application of streptomyces in promoting the formation of aerobic granular sludge and/or improving the stability of the aerobic granular sludge.
Background
Aerobic Granular Sludge (AGS) is a granular activated sludge formed by self-agglomeration of microorganisms under aerobic conditions, which has many unique advantages such as regular shape, compact structure, good sedimentation performance, high organic load and toxicity tolerance, has an aerobic and anaerobic coupling structure, and can realize synchronous denitrification and dephosphorization effects. After the aerobic granular sludge is first cultured in the upflow sludge bed reactor from Mishima and Nakamura in 1991, the culture and application cases of the aerobic granular sludge are continuously reported.
At present, most of the culture of aerobic granular sludge is carried out in a Sequencing Batch Reactor (SBR), and the reactor shows excellent granulating capability, but the granulating time is long, the stability of the granules is still to be improved, and the wide application of the aerobic granular sludge technology is limited. In order to promote the formation of aerobic granular sludge and improve the stability thereof, there is a patent that a functional filamentous fungus flora is constructed by constructing a filamentous yeast (including candida tropicalis, candida pinnata, candida lipolytica, and the like), a mould (including fusarium oxysporum, penicillium citrinum and aspergillus niger) and an actinomycete (streptomyces cellulolytic bacteria) according to a certain volume ratio, and then the functional filamentous fungus flora is utilized to react with activated sludge in a sequencing batch activated sludge reactor, so that the formation time of the aerobic granular sludge is shortened and the stability of the obtained aerobic granular sludge is improved. Although the method shortens the formation time of the aerobic granular sludge to a certain extent, the method still has an improved space, for example, the preparation of the used composite functional filamentous fungus flora is relatively complex, and is not as simple as that of using a single fungus. Therefore, further searching and culturing of the strain with good promoting effect is of great importance for the rapid culture of aerobic granular sludge.
Disclosure of Invention
The invention aims to overcome the defects and the shortcomings of the prior art, provides application of streptomyces in promoting formation of aerobic granular sludge and/or improving stability of the aerobic granular sludge, and provides a method for obtaining the aerobic granular sludge with good stability by rapid culture of the streptomyces on the basis.
A first object of the present invention is to provide the use of streptomyces in promoting the formation of and/or improving the stability of aerobic granular sludge.
A second object of the present invention is to provide the use of streptomyces in the preparation of a formulation for promoting the formation of and/or improving the stability of aerobic granular sludge.
The third method of the invention is to provide a method for culturing aerobic granular sludge by using streptomyces.
The above object of the present invention is achieved by the following technical scheme:
in the early experiments, the invention discovers that different strains or the same species but different strains have different promotion effects on forming aerobic granular sludge. Compared with a blank control, most strains can not effectively promote the formation of aerobic granular sludge, and even have no promoting effect. And the streptomycete with the preservation numbers of CICC 10513, CICC11035, CICC11010 and/or CICC21933 is utilized, so that the culture time of the aerobic granular sludge can be effectively shortened, and the cultured aerobic granular sludge has the advantages of regular appearance, smooth surface, regular shape, compact texture, stable structure and better stability.
Accordingly, the present application protects the use of streptomyces deposited under accession numbers cic 10513, cic 11035, cic 11010 and/or cic 21933 for promoting the formation of and/or improving the stability of aerobic granular sludge.
The invention also discloses application of the streptomyces in preparation of a preparation for promoting formation of aerobic granular sludge and/or improving stability of the aerobic granular sludge.
Specifically, the colony concentration of Streptomyces is 10 5 ～10 9 CFU/mL。
More specifically, the colony concentration of Streptomyces is 10 6 ～10 8 CFU/mL。
The invention also provides a method for culturing aerobic granular sludge by using streptomyces, which comprises the following steps:
s1, culturing streptomycete: culturing under aseptic condition to obtain streptomycete granule bacterial liquid;
s2, culturing aerobic granular sludge: and (3) adding the streptomycete granular bacterial liquid prepared in the step (S1) into activated sludge for culture to obtain aerobic granular sludge.
Specifically, the streptomycete is streptomycete with deposit numbers of CICC 10513, CICC11035, CICC11010 and/or CICC 21933.
Specifically, the temperature of the culture of the streptomycete granular bacterial liquid in the step S1 is 25-30 ℃.
Specifically, the shaking rate of the culture Streptomyces grain bacterial liquid in the step S1 is 200r/min.
Specifically, the culture medium used for culturing the streptomyces granular bacterial liquid in the step S1 is a liquid culture medium of Gaoshi No. 1.
The Gao's liquid culture medium contains soluble starch with the concentration of 20g/L, 1g/L potassium nitrate, 0.5g/L dipotassium hydrogen phosphate, 0.5g/L magnesium sulfate, 0.5g/L sodium chloride and 0.01g/L ferrous sulfate, and the pH value is 7.2-7.4.
Specifically, the colony concentration in the Streptomyces bacterial liquid in the step S1 is 10 5 ～10 9 CFU/mL。
Preferably, the colony concentration in the Streptomyces bacterial liquid in the step S1 is 10 6 ～10 8 CFU/mL。
Specifically, in step S2, the volume ratio of the activated sludge to the streptomycete is 100:25 to 100.
Preferably, the volume ratio of the activated sludge to the streptomyces in the step S2 is 100:25 to 80 percent.
Specifically, in the step S2, if the aerobic granular sludge is cultured by a shaking culture method, the culture condition is 25-30 ℃ and 150-250 r/min.
Specifically, in step S2, when the membrane bioreactor containing activated sludge is used for culturing aerobic granular sludge, the sludge load of the reactor is 0.1-0.4 gCOD/gMLSS.d, the initial concentration of the suspension solid concentration of the mixed solution is 2000-3000 mg/L, the DO of the aerobic zone is 2.0-4.0 mg/L, and the DO of the anaerobic zone is 0-2.0 mg/L.
Specifically, the membrane bioreactor is an inner loop membrane bioreactor or an integrated moving bed-membrane bioreactor.
Based on the practical application of the aerobic granular sludge, the application of the aerobic granular sludge prepared by any method of the invention in denitrification and dephosphorization of waste (sewage) water and/or treatment of polluted water is also within the protection scope of the invention.
The invention has the following beneficial effects:
the invention provides application of streptomyces with deposit numbers of CICC 10513, CICC11035, CICC11010 and/or CICC21933 in promoting formation of aerobic granular sludge and/or improving stability of the aerobic granular sludge, and provides a method for preparing the aerobic granular sludge by using the streptomyces. The method can be used for culturing and obtaining the aerobic granular sludge within 6-12 days, and the surface of the aerobic granular sludge obtained by culturing is smooth, regular in shape, compact in texture and good in stability, and the stability of the aerobic granular sludge can be improved while the formation time of the aerobic granular sludge is shortened.
Drawings
FIG. 1 is a morphology diagram of Streptomyces grain bacterial liquid prepared in example 1.
FIG. 2 is a morphology diagram of the aerobic granular sludge prepared in example 1.
FIG. 3 is a graph showing the morphology of the aerobic granular sludge obtained by the blank culture of example 1 on day 100.
FIG. 4 is a graph showing the morphology of the aerobic granular sludge prepared in example 1 on day 100.
FIG. 5 shows the results of the change of the mixed liquor suspended solids concentration (MLSS) with time in the membrane bioreactor of example 1.
Detailed Description
The invention is further illustrated in the following drawings and specific examples, which are not intended to limit the invention in any way. Unless specifically stated otherwise, the reagents, methods and apparatus employed in the present invention are those conventional in the art.
Reagents and materials used in the following examples are commercially available unless otherwise specified.
The Gaoshi liquid culture medium used in the invention contains soluble starch with the concentration of 20g/L, 1g/L potassium nitrate, 0.5g/L dipotassium hydrogen phosphate, 0.5g/L magnesium sulfate, 0.5g/L sodium chloride and 0.01g/L ferrous sulfate, and the pH value is 7.2-7.4.
Example 1 cultivation of aerobic granular sludge by Streptomyces sp deposited under accession number CICC 10513
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (accession number CICC 10513) under aseptic condition, and shake culturing at 25deg.C under 200r/min for 24 hr to obtain colony concentration of 1×10 6 CFU/mL streptomycete granular bacterial liquid;
s2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:25, adding the streptomyces granular bacterial liquid obtained in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, and culturing under the conditions that the sludge load is 0.3g COD/gMLSS.d, the aerobic zone DO is 3.0mg/L, the anaerobic zone DO is 0.5mg/L and the initial concentration of mixed liquor suspended solid concentration (MLSS) is 3000 mg/L.
Blank control: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to tap water is 100:25, adding tap water (without adding streptomyces granular bacteria liquid) into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, and culturing under the conditions that the sludge load is 0.3g COD/gMLSS.d, the aerobic zone DO is 3.0mg/L, the anaerobic zone DO is 0.5mg/L and the initial concentration of mixed liquor suspended solid concentration (MLSS) is 3000 mg/L.
The morphology graph of the streptomycete particle bacterial liquid prepared in the embodiment is shown in fig. 1, and as can be seen from fig. 1, the streptomycete particle obtained by culturing the streptomycete particle bacterial liquid in a liquid culture medium of Gao's No. 1 for 24 hours has smooth surface and regular shape, and shows that the streptomycete with the preservation number of CICC 10513 has the property of rapid granulation.
In the embodiment, the streptomycete granular bacterial liquid with the storage number of CICC 10513 is added into the activated sludge, and the aerobic granular sludge is prepared after 6 days of culture, and compared with a blank control (the aerobic granular sludge is cultured on the 39 th day), the culture time of the aerobic granular sludge is shortened by 33 days. The form of the aerobic granular sludge obtained by culturing for 6 days in the embodiment is shown in figure 2, and as can be seen from figure 2, the aerobic granular sludge obtained by culturing has regular shape, clear outline and compact structure, which shows that the stability of the aerobic granular sludge is good. The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using the streptomycete with the preservation number of CICC 10513, and the stability of the aerobic granular sludge obtained by culture is good.
In addition to the aerobic granular sludge obtained by culture, the invention also observes the stability of the aerobic granular sludge obtained by culture. After the aerobic granular sludge is obtained by culturing, the aerobic granular sludge is taken out at the same time every day, and is observed by a microscope, and the stability difference of the aerobic granular sludge cultured by adding streptomycete and not adding streptomycete (blank control) is compared.
The results are shown in fig. 3 and 4, respectively. FIG. 3 is a graph showing the morphology of the aerobic granular sludge obtained by the blank culture in the present example on day 100. As can be seen from FIG. 3, the aerobic granular sludge obtained by the culture without adding Streptomyces is cracked on day 100. Fig. 4 is a morphology diagram of the aerobic granular sludge prepared in this example on day 100, and as can be seen from fig. 4, the structure of the aerobic granular sludge cultured in this example remains stable on day 100. The comparison shows that the stability of the aerobic granular sludge obtained by culturing the streptomyces with the preservation number of CICC 10513 is better, which indicates that the stability of the cultured aerobic granular sludge can be improved by using the streptomyces with the preservation number of CICC 10513.
Example 2 cultivation of aerobic granular sludge Using Streptomyces sp with deposit number CICC 10513
This embodiment differs from embodiment 1 in that: the volume ratio of the activated sludge to the streptomycete granular bacterial liquid is 100:60.
the aerobic granular sludge is obtained after 8 days of culture in the embodiment, and the aerobic granular sludge obtained by culture has regular shape, clear outline and compact structure.
Example 3 cultivation of aerobic granular sludge Using Streptomyces sp with deposit number CICC 10513
This embodiment differs from embodiment 1 in that: the volume ratio of the activated sludge to the streptomycete granular bacterial liquid is 100:75.
the aerobic granular sludge is obtained after 10 days of culture in the embodiment, and the aerobic granular sludge obtained by culture has regular shape, clear outline and compact structure.
Example 4 cultivation of aerobic granular sludge Using Streptomyces sp with accession number CICC11035
This embodiment differs from embodiment 1 in that: the Streptomyces used in this example was deposited with the accession number CICC11035, which is different from the Streptomyces strain used.
In the embodiment, the aerobic granular sludge is obtained by adding the streptomyces granular bacterial liquid with the preservation number of CICC11035 into the activated sludge for 8 days, compared with a blank control, the formation time of the aerobic granular sludge is effectively shortened (31 days are shortened), the aerobic granular sludge obtained by culture is regular in shape, clear in outline and compact in structure, and after 100 days of culture, the result of the aerobic granular sludge is still stable and no cracking phenomenon is generated by observation under a microscope.
The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using streptomyces with the preservation number of CICC11035, and the stability of the aerobic granular sludge obtained by culture is good.
Example 5 cultivation of aerobic granular sludge Using Streptomyces sp with accession number CICC11010
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (with accession number CICC 11010) under aseptic condition, and shake culturing at 30deg.C and 200r/min for 36 hr to obtain colony concentration of 1×10 7 CFU/mL of Streptomyces granulous liquid.
S2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:50, adding the streptomycete granular bacterial liquid obtained in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, and culturing for 9 days under the conditions that the sludge load is 0.2g COD/gMLSS.d, the aerobic zone DO is 2.5mg/L, the anaerobic zone DO is 1.0mg/L and the MLSS initial concentration is 3000mg/L to obtain the aerobic granular sludge.
In the embodiment, the aerobic granular sludge is obtained by adding the streptomyces granular bacterial liquid with the preservation number of CICC11010 into the activated sludge for 9 days, compared with a blank control, the formation time of the aerobic granular sludge is effectively shortened, and the aerobic granular sludge obtained by the culture has regular shape, clear outline and compact structure, and does not generate cracking phenomenon after the culture is carried out for 100 days. The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using the streptomycete with the preservation number of CICC11010, and the stability of the aerobic granular sludge obtained by culture is good.
Example 6 cultivation of aerobic granular sludge with Streptomyces sp with deposit number CICC21933
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (with accession number CICC 21933) under aseptic condition at 25deg.C and 20Shake culturing at 0r/min for 48 hr to obtain colony with concentration of 1×10 8 CFU/mL of Streptomyces granulous liquid.
S2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:75, adding the streptomycete granular bacterial liquid obtained in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, and culturing for 10 days under the conditions that the sludge load is 0.1g COD/gMLSS.d, the aerobic zone DO is 3.0mg/L, the anaerobic zone DO is 0.5mg/L and the MLSS initial concentration is 2000mg/L to obtain the aerobic granular sludge.
In the embodiment, the aerobic granular sludge is obtained by adding the streptomycete granular bacterial liquid with the preservation number of CICC21933 into the activated sludge for 10 days, compared with a blank control, the formation time of the aerobic granular sludge is effectively shortened, and the aerobic granular sludge obtained by culturing has regular shape, clear outline and compact structure, and does not generate cracking phenomenon after culturing for 100 days. The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using the streptomycete with the preservation number of CICC21933, and the stability of the aerobic granular sludge obtained by culture is good.
Example 7 cultivation of aerobic granular sludge Using Streptomyces sp with accession number CICC11010
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (with accession number CICC 11010) under aseptic condition, and shake culturing at 30deg.C and 200r/min for 56 hr to obtain colony concentration of 2×10 6 CFU/mL of Streptomyces granulous liquid.
S2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:80, adding the streptomycete granular bacterial liquid obtained in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, the aerobic area DO is 4.0mg/L under the conditions that the sludge load is 0.4g COD/gMLSS.d, the anaerobic area DO is 1.5mg/L, the initial concentration of the MLSS is 2500mg/L, the aerobic granular sludge is obtained after culturing for 11 days, and the aerobic granular sludge obtained after culturing has regular shape, clear outline and compact structure, and no cracking phenomenon of the aerobic granular sludge is observed after culturing for 100 days.
The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using the streptomycete with the preservation number of CICC11010, and the stability of the aerobic granular sludge obtained by culture is good.
Example 8 cultivation of aerobic granular sludge with Streptomyces sp with deposit number CICC21933
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (with accession number CICC 21933) under aseptic condition, and shake culturing at 25deg.C under 200r/min for 48 hr to obtain colony concentration of 1×10 8 CFU/mL of Streptomyces granulous liquid.
S2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:60, adding the streptomycete granular bacterial liquid obtained in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, the aerobic area DO is 3.0mg/L under the conditions that the sludge load is 0.1g COD/gMLSS.d, the anaerobic area DO is 0.5mg/L, the initial concentration of the MLSS is 2500mg/L, the aerobic granular sludge is obtained after 10 days of culture, and the aerobic granular sludge obtained after culture has regular shape, clear outline and compact structure, and no cracking phenomenon of the aerobic granular sludge is observed after 100 days of culture.
The results show that the culture time for obtaining the aerobic granular sludge can be effectively shortened by using the streptomycete with the preservation number of CICC21933, and the stability of the aerobic granular sludge obtained by culture is good.
Example 9 cultivation of aerobic granular sludge Using Streptomyces sp with deposit number CICC 10513
S1, culturing streptomycete: culturing Streptomyces gao I liquid culture medium (accession number CICC 10513) under aseptic condition, and shake culturing at 25deg.C under 200r/min for 60 hr to obtain colony concentration of 2×10 8 CFU/mL of Streptomyces granulous liquid.
S2, culturing aerobic granular sludge: a plurality of conical flasks containing activated sludge are placed on a shaking table, and the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:70, adding the streptomycete granular bacterial liquid obtained in the step S1 into a conical flask, setting the rotating speed of a shaking table to be 200r/min, and culturing at room temperature (25 ℃) for 12 days under the condition that the initial concentration of MLSS of the added sludge is 2500mg/L to obtain aerobic granular sludge, wherein the aerobic granular sludge obtained by culturing has regular shape, clear outline and compact structure, and no cracking phenomenon of the aerobic granular sludge is observed after long-time culture.
Example 10 cultivation of aerobic granular sludge Using Streptomyces sp with accession number CICC11010
This example differs from example 9 in that the Streptomyces species used in this example are deposited under the accession number CICC 11010.
In the embodiment, the aerobic granular sludge is obtained by adding streptomycete granular bacterial liquid with the preservation number of CICC11010 into the activated sludge and culturing for 10 days, and the aerobic granular sludge obtained by culturing has regular shape, clear outline and compact structure, and no cracking phenomenon of the aerobic granular sludge is observed after long-time culturing.
Example 11 cultivation of aerobic granular sludge with Streptomyces sp with deposit number CICC21933
This example differs from example 9 in that the Streptomyces species used in this example are of the type having the deposit number CICC 21933.
In the embodiment, the aerobic granular sludge is obtained by adding the streptomycete granular bacterial liquid with the preservation number of CICC21933 into the activated sludge and culturing for 11 days, and the aerobic granular sludge obtained by culturing has regular shape, clear outline and compact structure, and the cracking phenomenon of the aerobic granular sludge is not observed after long-time culturing.
Test example 1 growth rate of aerobic granular sludge in membrane bioreactor
100mL of an aerobic granular sludge mixed solution is taken in an aerobic zone of a Membrane Bioreactor (MBR) in example 1 by using a measuring cylinder, and is kept stand for 30min, the volume of supernatant and the volume of sludge sediment are measured, and the sludge volume index SVI is obtained by calculation. And filtering the mixed solution by using filter paper dried to constant weight, putting the mixed solution into an oven at 105 ℃ for drying for 2 hours, taking out the mixed solution, cooling the mixed solution in a drying oven to constant temperature and constant weight, and measuring the weight difference between the front and the rear of the filter paper to obtain the MLSS value.
The results of the change with time of the mixed liquor suspended solid concentration (MLSS) in the membrane bioreactor are shown in FIG. 5, and it is clear from the results shown in FIG. 5 that the MLSS in the membrane bioreactor of example 1 rapidly increases in a short time from 3000mg/L on day 1 to 6700mg/L on day 15, which indicates that the formed aerobic granular sludge can rapidly grow in the membrane bioreactor, and that the activity of the aerobic granular sludge is good.
Comparative example 1 cultivation of aerobic granular sludge by Streptomyces with accession number CICC 11025
S1, culturing streptomycete: culturing Streptomyces gao (accession number: CICC 11025) under aseptic condition with culture medium, and shake culturing at 30deg.C under 150r/min for 60 hr to obtain colony concentration of 3×10 4 CFU/mL streptomycete granular bacterial liquid;
s2, culturing aerobic granular sludge: starting a membrane bioreactor containing activated sludge, wherein the volume ratio of the activated sludge to the streptococcal particle bacterial liquid is 100:80, adding the streptomycete granular bacterial liquid in the step S1 into a membrane bioreactor, wherein the membrane bioreactor is arranged to run in a continuous flow mode, culturing is carried out under the conditions that the sludge load is 0.5g COD/gMLSS.d, the aerobic zone DO is 3.0mg/L, the anaerobic zone DO is 2.5mg/L and the initial concentration of the MLSS is 3000mg/L, and no aerobic granular sludge is formed by day continuous observation until 20 days.
Comparative example 2 cultivation of aerobic granular sludge by Streptomyces with accession number CICC 24623
S1, culturing streptomycete: culturing Streptomyces gao (accession number CICC 24623) under aseptic condition, and shake culturing at 25deg.C under 200r/min for 56 hr to obtain colony concentration of 2×10 7 CFU/mL streptomycete granular bacterial liquid;
s2, culturing aerobic granular sludge: a plurality of conical flasks containing activated sludge are placed on a shaking table, and the volume ratio of the activated sludge to streptomycete particle bacterial liquid is 100:60, adding the streptomycete granular bacterial liquid obtained in the step S1 into a conical flask, setting the rotating speed of a shaking table to be 150r/min, culturing at room temperature (25 ℃) under the condition that the initial concentration of MLSS of the added sludge is 2500mg/L, and continuously observing every day until no aerobic granular sludge is formed on the 25 th day.
The above examples are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above examples, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present invention should be made in the equivalent manner, and the embodiments are included in the protection scope of the present invention.
Claims (5)
1. The application of streptomyces in promoting the formation of aerobic granular sludge and improving the stability of the aerobic granular sludge is characterized in that the streptomyces is the streptomyces with the preservation numbers of CICC 10513, CICC11035, CICC11010 and/or CICC 21933; the method for culturing the aerobic granular sludge by using the streptomyces comprises the following steps:
s1, culturing streptomycete: culturing under aseptic condition to obtain streptomycete granule bacterial liquid; wherein the colony concentration in the streptomycete particle bacterial liquid is 10 5 ～10 9 CFU/mL；
S2, culturing aerobic granular sludge: adding the streptomycete granular bacterial liquid prepared in the step S1 into activated sludge for culture to obtain aerobic granular sludge; wherein, the volume ratio of the activated sludge to the streptomycete particle bacterial liquid is 100: 25-100;
in the step S2, if the aerobic granular sludge is cultured by a shaking culture method, the culture condition is 25-30 ℃ and 150-250 r/min.
2. A method for culturing aerobic granular sludge by using streptomyces, which is characterized by comprising the following steps:
s1, culturing streptomycete: culturing under aseptic condition to obtain streptomycete granule bacterial liquid; wherein the colony concentration in the streptomycete particle bacterial liquid is 10 5 ～10 9 CFU/mL; the streptomycete is streptomycete with the preservation numbers of CICC 10513, CICC11035, CICC11010 and/or CICC 21933;
s2, culturing aerobic granular sludge: adding the streptomycete granular bacterial liquid prepared in the step S1 into activated sludge for culture to obtain aerobic granular sludge; wherein, the volume ratio of the activated sludge to the streptomycete particle bacterial liquid is 100: 25-100;
in the step S2, if the aerobic granular sludge is cultured by a shaking culture method, the culture condition is 25-30 ℃ and 150-250 r/min.
3. The method according to claim 2, wherein the culturing temperature of the Streptomyces pellet culture in step S1 is 25 to 30 ℃.
4. The method according to claim 2, wherein the medium used for culturing the Streptomyces grain bacterial liquid in step S1 is liquid medium of Khaki No. 1.
5. The method according to claim 2, wherein in step S2, if the aerobic granular sludge is cultured by using the membrane bioreactor containing activated sludge, the membrane bioreactor is operated in a continuous flow mode, the sludge load of the membrane bioreactor is 0.1 to 0.4g cod/gmlss.d, the initial concentration of the suspended solid concentration of the mixed liquor is 2000 to 3000mg/L, the aerobic zone DO is 2.0 to 4.0mg/L, and the anaerobic zone DO is 0 to 0.5mg/L.
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