CN101698534A - Method for optimizing stability of aerobic granular sludge - Google Patents
Method for optimizing stability of aerobic granular sludge Download PDFInfo
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- CN101698534A CN101698534A CN200910309565A CN200910309565A CN101698534A CN 101698534 A CN101698534 A CN 101698534A CN 200910309565 A CN200910309565 A CN 200910309565A CN 200910309565 A CN200910309565 A CN 200910309565A CN 101698534 A CN101698534 A CN 101698534A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention provides a method for optimizing stability of aerobic granular sludge in the technical field of environmental protection. The method comprises: constructing functional filamentous fungi flora; starting a sequencing-batch aerobic granular sludge reactor; feeding the functional filamentous fungi flora to the sequencing-batch aerobic granular sludge reactor by the microbial biomass; ensuring that the volume ratio of active sludge to the functional filamentous fungi flora in the sequencing-batch aerobic granular sludge reactor is 100:2-8; and controlling the sequencing-batch aerobic granular sludge reactor to prepare aerobic granular sludge. The method has the advantages of simplifying operating process, reducing capital-construction investment and operating cost and obviously improving the water quality of effluent.
Description
Technical field
What the present invention relates to is a kind of method of environmental protection technical field, specifically is a kind of method for optimizing stability of aerobic granular sludge.
Background technology
Nitrogen and phosphorus pollution is one of principal element of China's body eutrophication, wherein attaches most importance to ammonia and nitrogen pollution especially: become respectively in densely populated Huaihe River, the Yangtze valley and be ranked first and the 2nd contamination index; And in Taihu Lake of extensively being paid close attention to and Dian Chi, although the permanganate index of most lake surfaces has belonged to III class water, because the nitrogen and phosphorus pollution severe overweight, overall water quality still belongs to V class or even bad V class.The body eutrophication that ammonia and nitrogen pollution inspires causes algae to multiply in a large number, and dissolved oxygen concentration reduces in the water body, and the algae toxin concentration raises, the corrupt smelly situation of hydrobiont mass mortality and even generation, even make whole water body lose due ecological functions.On the other hand, the ammonia nitrogen that enters into natural water body is become nitrate and nitrite by bacterial oxidation easily, also can cause dissolved oxygen concentration decline in the water body, the harm water ecological setting; The nitrate of tap water middle and high concentration and nitrite then can cause baby's methemoglobinemia and nitrosamine type cancer.Therefore, the sewage water denitrification of the tertiary treatment of sewage, particularly city and surrounding area has become control and has improved the key of pollution situation such as China's body eutrophication, and exploiting economy denitrification process efficiently then is the only way of reaching this purpose.
Because there are many shortcomings such as technology is loaded down with trivial details, treatment efficiency is low, running maintenance complexity in existing wastewater biological denitrificaion technology, and the eutrophication pollution that countries in the world face is severe day by day, and the researchist has carried out many effort for this reason and successfully developed novel denitrification process such as SHARON, CANON, ANAMMOX etc.But these technologies all only are effective in the purification of ammonia nitrogen pollutant, also promptly all need to finish organic removing in the waste water in early stage, thereby cause unfavorable situations such as technology is loaded down with trivial details, occupation of land is wide, investment is big, operation and maintenance cost height.The aerobic particle mud technology of Ti Chuing was owing to parameters such as nutrition in the SBR cycle of operation and dissolved oxygen change greatly in the last few years, particularly the microorganism on the different loci is subjected to mass transport affects and faces different nutrition and dissolved oxygen environment in the granule sludge, therefore has the ability of the de-carbon of denitrogenation simultaneously.Does yet this technology lock into its effect main body mark ether handkerchief N peaceful of spraining? cultivate that acclimation period is long, operation stability is still unsatisfactory) and still fail to be promoted in actual applications.
Find through literature search prior art, Li Xiaomings etc. are in " Bioresource technology " (Biological resources technology, 2009, the Enhanced aerobic sludge granulation in sequencingbatch reactor by Mg that delivers on 100:64-67)
2+Augmentation (strengthening granular sludge in the batch type aerobic particle sludge reactor by adding magnesium ion) mentions the magnesium ion that adds 10mg/L in the batch type aerobic particle sludge reactor and can accelerate the granular sludge process, but fails effectively to improve the stability of granule sludge.China's application number is that the rare earth element solution of having put down in writing in 200610069502.5 the patent application by regularly add 30mg/L in the batch type aerobic particle sludge reactor promotes the method that granule sludge forms, same not mentioned influence to granule sludge stability.
Summary of the invention
The present invention is directed to the prior art above shortcomings, a kind of method for optimizing stability of aerobic granular sludge is provided, by in the batch type aerobic particle sludge reactor, adding functional filamentous fungus flora, can shorten granule sludge and form the required time, and can significantly improve concentration, density, subsidence rate and the metabolic activity of mud in the system, make in the time of work simplification that effluent quality obviously improves.
The present invention is achieved by the following technical solutions, the present invention includes following steps:
The first step, the first step, adopt malt extract medium, Gause I substratum and czapek's solution to cultivate respectively filamentous yeast, mould and actinomycetes and be configured to functional filamentous fungus flora, concrete steps comprise: cultivate 24h with the oscillation rate of 100-200rpm earlier in 28-35 ℃ shaking culture case and reach 10 to the bacterial concentration sum total
9Individual/L; Then by 5-8: 1-3: the 1-2 volume ratio is mixed, and makes functional filamentous fungus flora.
Described filamentous yeast is a kind of or its combination in the unusual mutation of unusual debaryomyces hansenii, candida tropicalis, skin shape trichosporon, Candida lipolytica, Fa Shi candiyeast or the Candida catenulata of filamentous yeast.
Described mould is a kind of or its combination in penicillium citrinum, black-koji mould or the sharp spore sickle mycete.
Described actinomycetes are for separating streptomyces cellulosae.
Described malt extract medium is not for to add the fermentation beer raw material of hops through being diluted to 12brix;
The component of described czapek's solution and content thereof are: sucrose 30g/L, NaNO
32g/L, K
2HPO
41g/L, MgSO
47H
2O 0.5g/L, KCl 0.5g/L and FeSO
47H
2O 0.1g/L, the pH of this czapek's solution are 7.0～7.2;
The component of described Gause I substratum and content thereof are: Zulkovsky starch 20g/L, KNO
31g/L, K
2HPO
40.5g/L, MgSO
47H
2O 0.5g/L, NaCl 0.5g/L and FeSO
40.01g/L the pH of this Gause I substratum is 7.2～7.4.
Second step, start the batch type aerobic particle sludge reactor that includes active sludge, in the batch type aerobic particle sludge reactor, add functional filamentous fungus flora in microbial biomass, make that active sludge and functional filamentous fungus flora volume ratio are 100 in the batch type aerobic particle sludge reactor: 2-8, control the batch type aerobic particle sludge reactor made then and obtain aerobic particle mud.
Described active sludge is taken from the aeration tank of municipal sewage plant, its component and content comprise: middle Escherichia 10～20%, give birth to that the moving glue bacterium 5～10% of branch, genus bacillus 5～10%, intestinal bacteria 5～10%, pseudomonas are about 5～10%, Alcaligenes is about 5～10%, Flavobacterium 1～5%, Aerobacter aerogenes 1～5%, the consumption of this active sludge is the volumetrical 20%～30% of batch type aerobic particle sludge reactor.
Described control batch type aerobic particle sludge reactor made is meant: the cycle of operation that control batch type aerobic particle sludge reactor is set is 4h-12h, the ratio of aerobic aeration time and anaerobism churning time is about 4-6 in the cycle of operation: 1, sludge loading is 0.5-0.7gCOD/gMLSS, DO is that 0.5-3.0mg/L tames cultivation, the aerobic particle mud of cultivating after being optimized through 7 to 20 days.
The concrete mechanism of action of the present invention is: the filamentous fungus own vol is big, the generation cycle is longer; " inert core " that in the granular sludge process, can serve as primary particles mud as free microorganism fixed carrier; simultaneously; the mycelia of filamentous fungus is subjected to shear action and self mutual winding in pelletising process, swept along other free microorganism therebetween and effectively fixed.And then the differentiation of accelerating system internal fixing microorganism and free microorganism under suitable waterpower selective action, effectively promoted granule sludge formation.For the granule sludge of being cultivated, its intravital filamentous fungus continues to serve as the network skeleton of granule sludge, and the outer polymer of the born of the same parents that produce in the microbial metabolism is " gel aqueous phase ", other ball, bar, chain microorganism then are dispersed in this " gel aqueous phase " and are fixed, thereby have strengthened the stability of granule sludge.
Microorganism that the present invention relates to and preservation information thereof comprise:
Filamentous yeast: Hansenula anomala (SCTCC 300237 for the unusual unusual mutation of debaryomyces hansenii, 2007-12-19);
Candida tropicalis (candida tropicalis, 2006-06-07, CICIM Y0239);
Trichosporon cutaneum (SCTCC 300239 for skin shape trichosporon, 2007-12-19);
Candida lipolytica (NK 3.00131 for Candida lipolytica, 2007-07-16);
Candida famata (NK 3.00136 for Fa Shi candiyeast, 2007-07-17);
Candida catenulate (Candida catenulata, 2006-06-07, CICIM Y0225);
Mould that the present invention relates to and preservation information thereof comprise:
Penicillium citrinum (NK 3.00057 for Penicillium citrinum, 2007-07-19);
Aspergillus Niger (NK 3.00046 for aspergillus niger, 1985-06-20);
Fusarium oxysporum (sharp spore reaping hook is mould, 2008-12-26, NK 3.00069).
Actinomycetes and the information thereof that the present invention relates to comprise:
Streptomyces cellulolyticus (separate streptomyces cellulosae, the thorough cellulolytic bacterial strain of a kind of energy that Dalian Polytechnic College filters out in research cellulose degradation process is published at Int.J.Syst.Bact., and 1997, on the 47:443-445).
Mentioned microorganism and mould all are preserved in Chinese typical culture collection center.
The present invention can effectively quicken the formation of aerobic particle mud, make its culture cycle not add month of functional flora and shorten to a week by contrast, sludge concentration improves 20-100%, and the granule sludge of being cultivated has good simultaneous denitrification de-carbon usefulness, be respectively 800mg/L and 160mg/L at water inlet COD and ammonia nitrogen concentration, hydraulic detention time is under the condition of 8h, can realize the synchronous removal of 95%COD and 98% ammonia nitrogen, and its nitrogen removal rate also reaches more than 90%.The granule sludge batch type aerobic particle sludge reactor that adopts this method to start lasts 3 years, and granule sludge is stable existence still, and the clearance of water inlet carbon nitrogen is stabilized in 95 ± 1% (COD) and 98 ± 2.5% (ammonia nitrogens).Than traditional denitrogenation de-carbon technology, because the simplification of technology, its floor space is saved more than 50%, and initial cost and working cost reduce about 50% on year-on-year basis, and effluent quality obviously improves, and this has important practical significance for the carbon polluted by nitrogen that present puzzlement China quality of water environment improves.
Embodiment
Below embodiments of the invention are elaborated, present embodiment is being to implement under the prerequisite with the technical solution of the present invention, provided detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Inoculation 1L ordinary activated sludge in laboratory scale batch type aerobic particle sludge reactor, drop into 50mL mixing functions sex pilus shape fungi bacterium liquid simultaneously, this functional filamentous fungus liquid comprises: filamentous yeast Hansenula anomala, Candidatropicalis, Trichosporon cutaneum, Candida lipolytica, Candida famata, Candidacatenulate, mould: Penicillium citrinum, Aspergillus Niger, Fusarium oxysporum and actinomycetes Streptomyces cellulolyticus (adopt malt extract medium respectively, Gause I substratum and czapek's solution are cultivated 24h with the oscillation rate of 100-200rpm and are reached 10 to the bacterial concentration sum total in 28-35 ℃ shaking culture case
9Individual/L, be to be mixed at 6: 2: 1 to form by filamentous yeast, mould and actinomycetic volume ratio then).With the simulation artificial wastewater is that matrix is carried out aerobic particle mud domestication cultivation; (the control batch type aerobic particle sludge reactor cycle of operation is 6h to operating parameterss such as the good/anaerobic condition by Adjustment System, aeration speed, the cycle of operation, organic loading; wherein aerobic 5h anaerobism 1h; sludge loading is 0.5gCOD/gMLSS; DO is that 1.5mg/L tames cultivation), the granulating process of mud in the batch type aerobic particle sludge reactor has promptly been finished in the operation in a week.
Described active sludge is taken from the aeration tank of municipal sewage plant, its component and content comprise: middle Escherichia 10%, give birth to that the moving glue bacterium 5% of branch, genus bacillus 5%, intestinal bacteria 5%, pseudomonas are about 5%, Alcaligenes is about 5%, Flavobacterium 1%, Aerobacter aerogenes 1%, the consumption of this active sludge is the volumetrical 20% of batch type aerobic particle sludge reactor.
In contrast to the batch type aerobic particle sludge reactor that does not add the functional microbial bacterium, the granule sludge that this batch type aerobic particle sludge reactor is cultivated has many advantages such as formation time is short, profile is regular, structure is tight, microbial population is abundant, biological activity height, it is about 100%, 50% and 50% that its MLSS, subsidence rate, degrading activity have improved respectively, and the biological activity that of particular concern is difficult degradation matrix such as its lignin degrading, Mierocrystalline cellulose, phenol is far above to (be about in the same old way 250%) in the same old way.
Embodiment 2
Inoculation 1L ordinary activated sludge in laboratory scale batch type aerobic particle sludge reactor, drop into 20mL mixing functions sex pilus shape fungi bacterium liquid simultaneously, this functional filamentous fungus liquid comprises: filamentous yeast Hansenula anomala, Candidatropicalis, Trichosporon cutaneum, Candida lipolytica, Candida famata, Candidacatenulate, mould: Penicillium citrinum, Aspergillus Niger, Fusarium oxysporum and actinomycetes Streptomyces cellulolyticus; Adopt malt extract medium, Gause I substratum and czapek's solution in 28-35 ℃ shaking culture case, to cultivate 24h respectively and reach 10 to the bacterial concentration sum total with the oscillation rate of 100-200rpm
9Individual/L, be to be mixed at 8: 1: 1 to form then by filamentous yeast, mould and actinomycetic volume ratio.With the simulation artificial wastewater is that matrix is carried out aerobic particle mud domestication cultivation; (the control batch type aerobic particle sludge reactor cycle of operation is 12h to operating parameterss such as the good/anaerobic condition (good in the cycle of operation/the anaerobism time) by Adjustment System, aeration speed, the cycle of operation, organic loading; wherein aerobic 9h anaerobism 3h; sludge loading is 0.6gCOD/gMLSS; DO is that 2.5mg/L tames cultivation), the granulating process of mud in the batch type aerobic particle sludge reactor has promptly been finished in the operation of fortnight.
Described active sludge is taken from the aeration tank of municipal sewage plant, its component and content comprise: middle Escherichia 20%, give birth to that the moving glue bacterium 10% of branch, genus bacillus 10%, intestinal bacteria 10%, pseudomonas are about 10%, Alcaligenes is about 10%, Flavobacterium 5%, Aerobacter aerogenes 5%, the consumption of this active sludge is the volumetrical 30% of batch type aerobic particle sludge reactor.
In contrast to the batch type aerobic particle sludge reactor that does not add functional microbial bacterium bacterium, the granule sludge that this batch type aerobic particle sludge reactor is cultivated has many advantages such as formation time is short, profile is regular, structure is tight, microbial population is abundant, biological activity height, it is about 20%, 40% and 20% that its MLSS, subsidence rate, degrading activity have improved respectively, and the biological activity that of particular concern is difficult degradation matrix such as its lignin degrading, Mierocrystalline cellulose, phenol is far above to (be about in the same old way 170%) in the same old way.
Embodiment 3
Inoculation 1L ordinary activated sludge in laboratory scale batch type aerobic particle sludge reactor, drop into 80mL mixing functions sex pilus shape fungi bacterium liquid simultaneously, this functional filamentous fungus liquid comprises: filamentous yeast Hansenula anomala, Candidatropicalis, Trichosporon cutaneum, Candida lipolytica, Candida famata, Candidacatenulate, mould: Penicillium citrinum, Aspergillus Niger, Fusarium oxysporum and actinomycetes Streptomyces cellulolyticus (adopt malt extract medium respectively, Gause I substratum and czapek's solution are cultivated 24h with the oscillation rate of 100-200rpm and are reached 10 to the bacterial concentration sum total in 28-35 ℃ shaking culture case
9Individual/L, be to be mixed at 5: 3: 2 to form by filamentous yeast, mould and actinomycetic volume ratio then).With the simulation artificial wastewater is that matrix is carried out aerobic particle mud domestication cultivation; (the control batch type aerobic particle sludge reactor cycle of operation is 8h to operating parameterss such as the good/anaerobic condition (good in the cycle of operation/the anaerobism time) by Adjustment System, aeration speed, the cycle of operation, organic loading; wherein aerobic 6h anaerobism 2h; sludge loading is 0.7gCOD/gMLSS; DO is that 3.0mg/L tames cultivation), the granulating process of mud in the batch type aerobic particle sludge reactor has promptly been finished in 10 days operations.
Described active sludge is taken from the aeration tank of municipal sewage plant, its component and content comprise: middle Escherichia 15%, give birth to that the moving glue bacterium 8% of branch, genus bacillus 8%, intestinal bacteria 8%, pseudomonas are about 8%, Alcaligenes is about 8%, Flavobacterium 3%, Aerobacter aerogenes 3%, the consumption of this active sludge is the volumetrical 25% of batch type aerobic particle sludge reactor.
In contrast to the batch type aerobic particle sludge reactor that does not add functional microbial bacterium bacterium, the granule sludge that this batch type aerobic particle sludge reactor is cultivated has many advantages such as formation time is short, profile is regular, structure is tight, microbial population is abundant, biological activity height, it is about 70%, 30% and 40% that its MLSS, subsidence rate, degrading activity have improved respectively, and the biological activity of difficult degradation matrix such as its lignin degrading, Mierocrystalline cellulose, phenol is equally far above to (be about in the same old way 210%) in the same old way.
Claims (9)
1. a method for optimizing stability of aerobic granular sludge is characterized in that, comprises the steps
The first step adopts malt extract medium, Gause I substratum and czapek's solution to cultivate respectively filamentous yeast, mould and actinomycetes and is configured to functional filamentous fungus flora;
Second step, start the batch type aerobic particle sludge reactor that includes active sludge, in the batch type aerobic particle sludge reactor, add functional filamentous fungus flora in microbial biomass, make that active sludge and functional filamentous fungus flora volume ratio are 100 in the batch type aerobic particle sludge reactor: 2-8, control the batch type aerobic particle sludge reactor made then and obtain aerobic particle mud.
2. method for optimizing stability of aerobic granular sludge according to claim 1, it is characterized in that, described cultivation is configured to functional filamentous fungus flora and is meant: cultivate 24h with the oscillation rate of 100-200rpm and reach 109/L to the bacterial concentration sum total in 28-35 ℃ shaking culture case, then by 5-8: 1-3: the 1-2 volume ratio is mixed, and makes functional filamentous fungus flora.
3. method for optimizing stability of aerobic granular sludge according to claim 1, it is characterized in that described filamentous yeast is a kind of or its combination in the unusual mutation of unusual debaryomyces hansenii, candida tropicalis, skin shape trichosporon, Candida lipolytica, Fa Shi candiyeast or the Candida catenulata of filamentous yeast.
4. method for optimizing stability of aerobic granular sludge according to claim 1 is characterized in that, described mould is a kind of or its combination in penicillium citrinum, black-koji mould or the sharp spore sickle mycete.
5. method for optimizing stability of aerobic granular sludge according to claim 1 is characterized in that described actinomycetes are for separating streptomyces cellulosae.
6. method for optimizing stability of aerobic granular sludge according to claim 1 is characterized in that described malt extract medium is not for to add the fermentation beer raw material of hops through being diluted to 12 brix.
7. method for optimizing stability of aerobic granular sludge according to claim 1 is characterized in that, the component of described czapek's solution and content thereof are: sucrose 30g/L, NaNO
32g/L, K
2HPO
41g/L, MgSO
47H
2O 0.5g/L, KCl 0.5g/L and FeSO
47H
2O 0.1g/L, the pH of this czapek's solution are 7.0～7.2.
8. method for optimizing stability of aerobic granular sludge according to claim 1 is characterized in that, the component of described Gause I substratum and content thereof are: Zulkovsky starch 20g/L, KNO
31g/L, K
2HPO
40.5g/L, MgSO
47H
2O 0.5g/L, NaCl 0.5g/L and FeSO
40.01g/L the pH of this Gause I substratum is 7.2～7.4.
9. method for optimizing stability of aerobic granular sludge according to claim 1, it is characterized in that, described control batch type aerobic particle sludge reactor made is meant: the cycle of operation that control batch type aerobic particle sludge reactor is set is 4h-12h, the ratio of aerobic aeration time and anaerobism churning time is about 4-6 in the cycle of operation: 1, sludge loading is 0.5-0.7gCOD/gMLSS, DO is that 0.5-3.0mg/L tames cultivation, the aerobic particle mud of cultivating after being optimized through 7 to 20 days.
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| US5486474A (en) * | 1991-02-01 | 1996-01-23 | Mycotech Corporation | Bioremediation method using a high nitrogen-containing culture of white rot fungi on sugar beet pulp |
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| CN114716007B (en) * | 2022-04-21 | 2023-11-03 | 广东工业大学 | Application of streptomycete in promoting formation of aerobic granular sludge and/or improving stability of aerobic granular sludge |
| CN114772715A (en) * | 2022-04-28 | 2022-07-22 | 广东工业大学 | Application of streptomycete and bacillus in promoting formation of aerobic granular sludge |
| CN114772715B (en) * | 2022-04-28 | 2023-11-03 | 广东工业大学 | Application of streptomycete and bacillus in promoting formation of aerobic granular sludge |
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