CN113173650A - Method for treating septic tank sewage by coupling compound microbial agent and calcium peroxide - Google Patents
Method for treating septic tank sewage by coupling compound microbial agent and calcium peroxide Download PDFInfo
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Abstract
The invention discloses a method for treating septic tank sewage by coupling compound microbial agent and calcium peroxide, which comprises four steps of adding modified calcium peroxide, culturing compound microbial agent, domesticating compound microbial agent and treating sewage. Wherein the compound microbial agent comprises four microbial agents of aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes. The modified calcium peroxide is used, so that the oxygen release is more stable and long-acting; meanwhile, compared with the method of directly adopting unmodified calcium peroxide, the modified calcium peroxide can maintain the pH value of the system between 8.0 and 8.5, maintain a relatively stable external environment, meet the growth requirement of microbial colonies and improve the decontamination efficiency of bacterial colonies. The removal rate of COD in sewage by coupling the compound microbial agent and the modified calcium peroxide reaches 60-70%, the removal rate of ammonia nitrogen reaches 90-95%, the removal rate of total phosphorus reaches 90-95%, the removal rate of total nitrogen reaches 90-93%, and the efficiency is greatly improved compared with that of the traditional treatment mode.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a method for treating sewage of a septic tank by coupling a compound microbial agent and calcium peroxide.
Background
With the continuous promotion of the new rural construction and urban and rural integrated process in China, the rural living standard and economic development are greatly improved. However, the development of rural environment construction is relatively delayed, and the treatment of rural domestic sewage becomes a focus of much attention in environmental protection.
At present, most rural domestic sewage is treated by adopting a traditional three-grid septic tank, and the proportion of the number of the three-grid septic tanks is more than 90 percent. Researches find that the water quality conditions of sewage in a rural septic tank are as follows: the biological oxygen demand (BOD5) is 180-320 mg/L, the Chemical Oxygen Demand (COD) is 265-510 mg/L, the solid suspension amount (SS) is 90-255 mg/L, and the ammoniacal Nitrogen (NH) is4 +-N) content of 20-60 mg/L, total nitrogen content of 25-80 mg/L and total phosphorus content of 1.5-5.0 mg/L. The three-grid septic tank has low removal rate of total nitrogen, total phosphorus and ammonia nitrogen, which is only about 3 percent, and has high Chemical Oxygen Demand (COD)The removal rate is about 15 percent, and the removal rate of solid suspended matters is about 30 percent, so that the effluent treated by the three-grid septic tank can not even reach the national sewage three-level discharge standard, and has a larger gap.
On the other hand, untreated sewage of the rural septic tank contains a large amount of organic matters, phosphorus, nitrogen and the like, and is discharged into a river channel nearby without standard treatment or enters the river after being converged by a drainage channel, so that a large amount of algae in a water body are propagated, and the eutrophication phenomenon is caused. At present, the sewage treatment mode of a rural septic tank is mainly direct discharge into rivers, access to a municipal sewage pipe network, centralized treatment by sewage plants, artificial wetland treatment and the like. The above processing methods all have the following problems:
(1) if the quality of the effluent treated by the septic tank can not reach the national third-level sewage discharge standard, the effluent contains a large amount of pollutants such as nitrogen, phosphorus and the like, and the water body is easily eutrophicated after entering rivers and lakes.
(2) If the sewage treatment system is connected to a municipal pipe network, the sewage treatment plant is used for centralized treatment, and the sewage treatment plant is not built in most rural areas due to the limitation of the rural development level, so that the feasibility is not achieved. Even possess sewage treatment plant, most are in the town area, and it is far away from rural area, and the pipe network is laid and is invested in great, and rural area relief is complicated, and the construction degree of difficulty is big, and the mode degree of difficulty that inserts municipal pipe network and handle at present is big on the whole, and the benefit is low.
(3) The artificial wetland treatment mode needs to occupy a large amount of land, has high management difficulty and is easy to cause secondary pollution.
In conclusion, due to the rural economic development level, the operation of the existing septic tank sewage treatment technology cannot be supported, and a new economical and efficient treatment means aiming at the septic tank sewage is urgently needed to be developed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method for treating septic tank sewage by coupling compound microbial inoculum and calcium peroxide, which is characterized in that the screened special strain types and the high-efficiency compound microbial inoculum prepared by optimized proportion are added in the sewage treatment process, and the special physical and chemical properties of modified calcium peroxide are combined, so that the composition and the function of the original microorganisms in the sewage are strengthened, the continuous re-decomposition of organic pollutants and the high-efficiency removal of nitrogen and phosphorus are promoted, and the method is suitable for the treatment of the sewage of the rural septic tank.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for treating septic tank sewage by coupling compound microbial inoculant and calcium peroxide comprises the following steps:
(1) adding modified calcium peroxide: adding modified calcium peroxide into the pretreated sewage;
(2) culturing a compound microbial agent: after the dissolved oxygen in the water body is stable, adding the compound microbial inoculum into the sewage, controlling the pH value of the sewage to be 7.0-8.0, the temperature to be 28-32 ℃ and the dissolved oxygen to be 3-5 mg/L, and culturing the compound microbial inoculum for 2-3 d;
(3) domesticating a compound microbial agent: adjusting the concentration of thallus in the sewage, controlling the pH value of the sewage to be 7.0-8.0 and the treated water amount to be 40-100 m3D, domesticating the compound microbial inoculum for 7-15 d, wherein the water temperature is 28-32 ℃, and the dissolved oxygen is 3-5 mg/L;
(4) treating sewage: controlling the pH value of the sewage to be 7.0-8.0, the water temperature to be 28-32 ℃, the dissolved oxygen to be 3-5 mg/L and the hydraulic retention time to be 8-24 h, thus finishing the treatment of the sewage;
wherein the compound microbial agent consists of aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes.
Preferably, the addition amount of the modified calcium peroxide in the step (1) is 0.5-0.8 per mill of the volume of the sewage.
Preferably, the adding amount of the compound microbial agent in the step (2) is 0.4-0.6 g/m3。
Preferably, the cell concentration in step (3) is 1X 120 to 6X 120 cells/mL.
Preferably, the amount of the treated water in the step (3) is 50m3The water temperature is 30 ℃, and the dissolved oxygen is 4.0 mg/L.
Preferably, in the step (4), the pH is 7.2, the water temperature is 30 ℃, the dissolved oxygen is 4.0mg/L, and the hydraulic retention time is 8 h.
Preferably, the preparation of the modified calcium peroxide comprises the following steps:
(11) CaO is weighed according to the proportion2Mixing the clean water sludge and the cement uniformly;
(12) preparing the mixture uniformly mixed in the step (11) into a sphere with the diameter of 0.5-1 cm;
(13) and (3) drying the spheres prepared in the step (12) at the temperature of 80-110 ℃ for 1-2 h to obtain the modified calcium peroxide.
Preferably, the prepared modified calcium peroxide has the sustained and stable oxygen release time of 25-30 d.
Preferably, the prepared modified calcium peroxide can maintain the pH value of a water body between 8.0 and 8.5, and provides a suitable environment for the growth and propagation of microorganisms.
Preferably, CaO2The mass ratio of the water purification sludge to the cement is 0.8-1.7: 1: 0.56 to 1.
Preferably, the preparation of the complex microbial agent comprises the following steps: sequentially inoculating aerobic denitrifying bacteria seed liquid, lactobacillus acidophilus seed liquid, bacillus subtilis seed liquid and actinomycetes seed liquid into a fermentation culture medium, performing fermentation culture for 36-48 h under the conditions that the pH is 7.0-8.2 and the temperature is 28-30 ℃ to obtain fermentation liquid, and drying the fermentation liquid to obtain the compound microbial agent.
Preferably, the aerobic denitrifying bacteria seed liquid, the lactobacillus acidophilus seed liquid, the bacillus subtilis seed liquid and the actinomycete seed liquid account for 25 to 30 percent, 3 to 5 percent, 5 to 8 percent and 15 to 20 percent of volume percentage respectively.
Preferably, the preparation of the aerobic denitrifying bacteria seed solution comprises the following steps: after activated culture of the aerobic denitrifying bacteria, inoculating the aerobic denitrifying bacteria into a shake flask culture medium I, and then culturing for 30-50 h at 28-32 ℃ to obtain an aerobic denitrifying bacteria seed solution.
Preferably, the shake flask culture medium I comprises the following components per liter: 13.2g/L of white sugar; 2g/L of potassium nitrate; 0.5g/L of monopotassium phosphate; magnesium sulfate heptahydrate 0.2 g/L; ferrous sulfate heptahydrate 0.05 g/L; 0.02g/L of calcium chloride; adjusting the pH value to 7.5-8.0.
Preferably, the preparation of the lactobacillus acidophilus seed liquid comprises the following steps: activating and culturing lactobacillus acidophilus, inoculating the lactobacillus acidophilus into a shake flask culture medium II, and then culturing for 36-48 h under the anaerobic condition at the temperature of 28-32 ℃ to obtain the lactobacillus acidophilus seed liquid.
Preferably, the shake flask culture medium II comprises the following components per liter: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Preferably, the preparation of the bacillus subtilis seed solution comprises the following steps: activating and culturing the bacillus subtilis, inoculating the bacillus subtilis into a shake flask culture medium III, and then culturing for 24-48 h at 35 ℃ to obtain a bacillus subtilis seed solution.
Preferably, the components per liter in the shake flask culture medium III are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Preferably, the preparation of the actinomycete seed liquid comprises the following steps: and (3) performing activated culture on the actinomycetes, inoculating the actinomycetes into a shake flask culture medium IV, and then culturing for 36-48 h at the temperature of 30-37 ℃ to obtain an actinomycetes seed solution.
Preferably, the shake flask culture medium IV comprises the following components per liter: 0.5g of soybean meal; 1.5g of ammonium sulfate; 2.5g of glucose; 0.6g of sodium chloride; 0.6g of calcium carbonate; adjusting the pH value to 7.0-7.5.
Preferably, the fermentation medium is prepared from the following components in percentage by weight:
the pH value of the fermentation medium is 7.0.
Preferably, the pretreated sewage is sewage of a septic tank, and comprises the following components in percentage by weight:
COD：100～300mg/L；
ammonia nitrogen: 200-400 mg/L;
total nitrogen: 300-500 mg/L;
total phosphorus: 20-50 mg/L.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention provides a method for treating septic tank sewage by coupling compound microbial agent and calcium peroxide, and creatively provides a method for coupling decontamination by adopting modified calcium peroxide and optimized compound microbial agent. The modified calcium peroxide is used, so that oxygen release is more stable and long-acting; meanwhile, compared with the method of directly adopting unmodified calcium peroxide, the modified calcium peroxide can maintain the pH value of the system to be 8.0-8.5, maintain a relatively stable external environment, meet the growth requirement of microbial colonies and improve the decontamination efficiency of bacterial colonies; meanwhile, the invention obtains the compound microbial agent (aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes) by adopting different microorganisms through co-fermentation, the compound microbial agent and the modified calcium peroxide are applied to the sewage treatment process of rural toilets together, the microbial community structure in the sewage treatment system of the septic tank is optimized, the composition and the function of the original microorganisms in the sewage are strengthened, the degradation of organic pollutants in the sewage and the removal of pollutants such as nitrogen and phosphorus can be obviously improved, the removal rate of COD in the sewage reaches 60-70%, the removal rate of ammonia nitrogen reaches 90-95%, the removal rate of total phosphorus reaches 90-95%, the removal rate of total nitrogen reaches 90-93%, and the efficiency is greatly improved compared with the traditional treatment mode.
(2) The modified calcium peroxide is used for oxygenation to replace the traditional sewage treatment aeration process, so that the energy consumption in the sewage treatment aeration process is greatly reduced, the energy consumption in the traditional sewage treatment aeration stage is 50% -70%, and the energy consumption can be saved by 30% -40% by using the modified calcium peroxide to oxygenate the aeration stage.
(3) The method adopts the modified calcium peroxide oxygenation agent, the calcium peroxide has stronger adsorption effect on phosphorus in the sewage, and partial by-products generated by the reaction of the calcium peroxide and water have stronger oxidizing capacity, so that the method has better removal effect on organic pollutants in the sewage.
Drawings
FIG. 1 is a graph showing the oxygen release curves of modified calcium peroxide and unmodified calcium peroxide prepared in example 1 of the present application;
fig. 2 is a comparison graph of the pH change of the water body of the modified calcium peroxide and the unmodified calcium peroxide prepared in example 1 of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly illustrated, the present invention will be further explained in detail with reference to the accompanying drawings.
It is particularly emphasized that the starting materials or reagents of the present invention are commercially available, unless otherwise specified. Wherein, the purified water sludge is obtained from a wastewater treatment plant of the Tangson lake.
Example 1
A method for treating septic tank sewage by coupling compound microbial inoculant and calcium peroxide comprises the following steps:
(1) adding modified calcium peroxide: adding modified calcium peroxide with the sewage volume of 0.5 per mill into the pretreated sewage;
(2) culturing a compound microbial agent: after the dissolved oxygen in the water body is stable, adding the compound microbial agent into the sewage, wherein the adding amount is 0.6g/m3Controlling the pH value of the sewage to be 7.0, the temperature to be 28 ℃ and the dissolved oxygen to be 4mg/L, and culturing the compound microbial agent 2 d;
(3) domesticating a compound microbial agent: adjusting the thallus concentration in the sewage to 4 multiplied by 120/mL, controlling the pH value of the sewage to 7.0 and the treated water amount to 50m3D, domesticating the compound microbial agent for 7d, wherein the water temperature is 28 ℃, and the dissolved oxygen is 4 mg/L;
(4) treating sewage: controlling the pH value of the sewage to be 7.0, the water temperature to be 28 ℃, the dissolved oxygen to be 4mg/L and the hydraulic retention time to be 8h, thus finishing the treatment of the sewage.
In the embodiment, the pretreated sewage is sewage of a septic tank in a certain rural area of the city of the peach, and the main components of the sewage are shown in table 1.
TABLE 1 average value of main components of septic tank sewage in a certain countryside of Hubei Xiantao city (unit: mg/L)
| Item | COD | Total nitrogen | Total phosphorus | Ammonia nitrogen | Nitro nitrogen |
| Numerical value | 234.5 | 554 | 43 | 231 | 2.1 |
Wherein, the preparation of the modified calcium peroxide comprises the following steps:
(1) 800g CaO was weighed21000g of pure water sludge and 600g of cement are uniformly mixed;
(2) preparing the mixture uniformly mixed in the step (1) into a sphere with the diameter of 0.6 cm;
(3) and (3) drying the spheres prepared in the step (2) at 90 ℃ for 2h to obtain the modified calcium peroxide.
As can be seen from the figures 1 and 2, the modified calcium peroxide prepared by the method has more stable and long-acting oxygen release and the pH value of 8.0-8.5.
Wherein, the compound microbial agent comprises aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes, and the preparation method comprises the following steps: inoculating aerobic denitrifying bacteria seed liquid, lactobacillus acidophilus seed liquid, bacillus subtilis seed liquid and actinomycetes seed liquid in a fermentation culture medium according to the volume percentage of 25%, 3%, 5% and 15% of strains in sequence, performing fermentation culture for 36 hours under the conditions that the pH is 7.0 and the temperature is 28 ℃ to obtain fermentation liquid, and drying the fermentation liquid to obtain the compound microbial agent.
Wherein the fermentation medium is prepared from the following components in percentage by weight:
wherein the pH value of the fermentation medium is 7.0.
The preparation method of the aerobic denitrifying bacteria seed liquid comprises the following steps: after activated culture of the aerobic denitrifying bacteria, inoculating the aerobic denitrifying bacteria into a shake flask culture medium I, and then culturing for 30 hours at 28 ℃ to obtain an aerobic denitrifying bacteria seed solution.
The preparation method of the lactobacillus acidophilus seed liquid comprises the following steps: activating and culturing lactobacillus acidophilus, inoculating the lactobacillus acidophilus into a shake flask culture medium II, and then culturing for 36 hours at 28 ℃ under an anaerobic condition to obtain lactobacillus acidophilus seed liquid.
The preparation method of the bacillus subtilis seed solution comprises the following steps: activating and culturing the bacillus subtilis, inoculating the bacillus subtilis into a shake flask culture medium III, and then culturing for 24 hours at 35 ℃ to obtain a bacillus subtilis seed solution.
The preparation method of the actinomycete seed liquid comprises the following steps: and (3) performing activated culture on the actinomycetes, inoculating the actinomycetes into a shake flask culture medium IV, and then culturing for 36 hours at the temperature of 30 ℃ to obtain an actinomycetes seed solution.
Wherein, the components per liter in the shake flask culture medium I are as follows: 13.2g/L of white sugar; 2g/L of potassium nitrate; 0.5g/L of monopotassium phosphate; magnesium sulfate heptahydrate 0.2 g/L; ferrous sulfate heptahydrate 0.05 g/L; 0.02g/L of calcium chloride; the pH was adjusted to 7.5.
Wherein, the components per liter in the shake flask culture medium II are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium III are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium IV are as follows: 0.5g of soybean meal; 1.5g of ammonium sulfate; 2.5g of glucose; 0.6g of sodium chloride; 0.6g of calcium carbonate; the pH was adjusted to 7.0.
The components of the treated sewage were measured, and the results are shown in Table 2.
TABLE 2 results of measurement of various components in the treated water (unit: mg/L)
| Item | COD | Total nitrogen | Total phosphorus | Ammonia nitrogen | Nitro nitrogen |
| Numerical value | 82 | 52 | 2.3 | 20 | 0.5 |
The detection result of the treated water shows that the combined use of the compound microbial agent and the modified calcium peroxide is relatively quick and effective in removing organic pollutants and nitrogen and phosphorus in the sewage. The removal rates of COD, total nitrogen, total phosphorus, ammonia nitrogen and nitrate nitrogen are respectively 65.0%, 90.6%, 94.7%, 91.3% and 76.2%, and the effluent of the septic tank meets the requirements of the national secondary standard of comprehensive sewage discharge.
Example 2
A method for treating septic tank sewage by coupling compound microbial inoculant and calcium peroxide comprises the following steps:
(1) adding modified calcium peroxide: adding modified calcium peroxide with the sewage volume of 0.8 per mill into the pretreated sewage;
(2) culturing a compound microbial agent: after the dissolved oxygen in the water body is stable, adding the compound microbial agent into the sewage, wherein the adding amount is 0.4g/m3Controlling the pH value of the sewage to be 7.5, the temperature to be 28 ℃ and the dissolved oxygen to be 5mg/L, and culturing the compound microbial agent for 3 d;
(3) domesticating a compound microbial agent: adjusting the thallus concentration in the sewage to 6 multiplied by 120/mL, controlling the pH value of the sewage to 7.5 and the treated water amount to 80m3D, domesticating the compound microbial agent for 7d, wherein the water temperature is 28 ℃, and the dissolved oxygen is 5 mg/L;
(4) treating sewage: controlling the pH value of the sewage to be 7.5, the water temperature to be 28 ℃, the dissolved oxygen to be 5mg/L and the hydraulic retention time to be 24h, thus finishing the treatment of the sewage.
In this example, the sewage to be pretreated is sewage from septic tanks in a certain countryside of the city of peach, and the main components are shown in table 3.
TABLE 3 average value of main components of septic tank sewage in a certain town of Hubei Xiantao City (unit: mg/L)
| Item | COD | Ammonia nitrogen | Total nitrogen | Total phosphorus |
| Numerical value | 252 | 224.1 | 482.8 | 35 |
Wherein, the preparation of the modified calcium peroxide comprises the following steps:
(1) weighing 1200g of CaO21000g of purified water sludge and 800g of cement are uniformly mixed;
(2) preparing the mixture uniformly mixed in the step (1) into a sphere with the diameter of 0.8 cm;
(3) and (3) drying the spheres prepared in the step (2) at 100 ℃ for 1h to obtain the modified calcium peroxide.
Wherein, the compound microbial agent comprises aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes, and the preparation method comprises the following steps: inoculating an aerobic denitrifying bacterium seed solution, a lactobacillus acidophilus seed solution, a bacillus subtilis seed solution and an actinomycete seed solution into a fermentation culture medium in sequence according to the volume percentage of 27%, 4%, 6% and 16% of strains, performing fermentation culture for 48 hours under the conditions that the pH is 7.2 and the temperature is 30 ℃ to obtain a fermentation liquid, and drying the fermentation liquid to obtain the compound microbial agent.
Wherein the fermentation medium is prepared from the following components in percentage by weight:
wherein the pH value of the fermentation medium is 7.0.
The preparation method of the aerobic denitrifying bacteria seed liquid comprises the following steps: after activated culture of the aerobic denitrifying bacteria, inoculating the aerobic denitrifying bacteria into a shake flask culture medium I, and then culturing the aerobic denitrifying bacteria at 30 ℃ for 40 hours to obtain an aerobic denitrifying bacteria seed solution.
The preparation method of the lactobacillus acidophilus seed liquid comprises the following steps: activating and culturing lactobacillus acidophilus, inoculating the lactobacillus acidophilus into a shake flask culture medium II, and then culturing for 48 hours under the anaerobic condition at the temperature of 30 ℃ to obtain lactobacillus acidophilus seed liquid.
The preparation method of the bacillus subtilis seed solution comprises the following steps: activating and culturing the bacillus subtilis, inoculating the bacillus subtilis into a shake flask culture medium III, and culturing at 35 ℃ for 36 hours to obtain a bacillus subtilis seed solution.
The preparation method of the actinomycete seed liquid comprises the following steps: and (3) performing activated culture on the actinomycetes, inoculating the actinomycetes into a shake flask culture medium IV, and then culturing for 48 hours at 32 ℃ to obtain an actinomycetes seed solution.
Wherein, the components per liter in the shake flask culture medium I are as follows: 13.2g/L of white sugar; 2g/L of potassium nitrate; 0.5g/L of monopotassium phosphate; magnesium sulfate heptahydrate 0.2 g/L; ferrous sulfate heptahydrate 0.05 g/L; 0.02g/L of calcium chloride; the pH was adjusted to 7.6.
Wherein, the components per liter in the shake flask culture medium II are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium III are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium IV are as follows: 0.5g of soybean meal; 1.5g of ammonium sulfate; 2.5g of glucose; 0.6g of sodium chloride; 0.6g of calcium carbonate; the pH was adjusted to 7.2.
The components of the treated sewage were measured, and the results are shown in Table 4.
TABLE 4 results of measurement of various components in the treated water (unit: mg/L)
| Item | COD | Total nitrogen | Total phosphorus | Ammonia nitrogen |
| Numerical value | 90.2 | 47.3 | 1.9 | 21.5 |
According to the detection table of the treated water, the combined use of the compound microbial agent and the modified calcium peroxide can remove organic pollutants and nitrogen and phosphorus in the sewage more quickly and efficiently. The removal rates of COD, total nitrogen, total phosphorus and ammonia nitrogen are respectively 64.2%, 90.2%, 94.6% and 90.4%, and the effluent of the septic tank meets the requirements of the national sewage comprehensive discharge second-level standard.
Comparative example 1
A method for treating septic tank sewage by coupling compound microbial inoculant and calcium peroxide comprises the following steps:
(1) adding calcium peroxide: adding calcium peroxide with the volume of 0.5 per mill of the sewage into the pretreated sewage (same as the example 1);
(2) culturing a compound microbial agent: after the dissolved oxygen in the water body is stable, adding the compound microbial agent into the sewage, wherein the adding amount is 0.6g/m3Controlling the pH value of the sewage to be 7.0, the temperature to be 28 ℃ and the dissolved oxygen to be 4mg/L, and culturing the compound microbial agent 2 d;
(3) domesticating a compound microbial agent: adjusting the thallus concentration in the sewage to 4 multiplied by 120/mL, controlling the pH value of the sewage to 7.0 and the treated water amount to 50m3D, domesticating the compound microbial agent for 7d, wherein the water temperature is 28 ℃, and the dissolved oxygen is 4 mg/L;
(4) treating sewage: controlling the pH value of the sewage to be 7.0, the water temperature to be 28 ℃, the dissolved oxygen to be 4mg/L and the hydraulic retention time to be 8h, thus finishing the treatment of the sewage.
Wherein, the compound microbial agent comprises aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes, and the preparation method comprises the following steps: inoculating aerobic denitrifying bacteria seed liquid, lactobacillus acidophilus seed liquid, bacillus subtilis seed liquid and actinomycetes seed liquid in a fermentation culture medium according to the volume percentages of 25%, 3%, 5% and 15%, fermenting and culturing for 36h under the conditions that the pH is 7.0 and the temperature is 28 ℃ to obtain fermentation liquid, and drying the fermentation liquid to obtain the compound microbial agent.
Wherein the fermentation medium is prepared from the following components in percentage by weight:
wherein the pH value of the fermentation medium is 7.0.
The preparation method of the aerobic denitrifying bacteria seed liquid comprises the following steps: after activated culture of the aerobic denitrifying bacteria, inoculating the aerobic denitrifying bacteria into a shake flask culture medium I, and then culturing for 30 hours at 28 ℃ to obtain an aerobic denitrifying bacteria seed solution.
The preparation method of the lactobacillus acidophilus seed liquid comprises the following steps: activating and culturing lactobacillus acidophilus, inoculating the lactobacillus acidophilus into a shake flask culture medium II, and then culturing for 36 hours at 28 ℃ under an anaerobic condition to obtain lactobacillus acidophilus seed liquid.
The preparation method of the bacillus subtilis seed solution comprises the following steps: activating and culturing the bacillus subtilis, inoculating the bacillus subtilis into a shake flask culture medium III, and then culturing for 24 hours at 35 ℃ to obtain a bacillus subtilis seed solution.
The preparation method of the actinomycete seed liquid comprises the following steps: and (3) performing activated culture on the actinomycetes, inoculating the actinomycetes into a shake flask culture medium IV, and then culturing for 36 hours at the temperature of 30 ℃ to obtain an actinomycetes seed solution.
Wherein, the components per liter in the shake flask culture medium I are as follows: 13.2g/L of white sugar; 2g/L of potassium nitrate; 0.5g/L of monopotassium phosphate; magnesium sulfate heptahydrate 0.2 g/L; ferrous sulfate heptahydrate 0.05 g/L; 0.02g/L of calcium chloride; the pH was adjusted to 7.5.
Wherein, the components per liter in the shake flask culture medium II are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium III are as follows: 20g of glucose; 15g of peptone; 5g of sodium chloride; 0.5g of beef extract; 20g of agar; the pH was adjusted to 7.0.
Wherein, the components per liter in the shake flask culture medium IV are as follows: 0.5g of soybean meal; 1.5g of ammonium sulfate; 2.5g of glucose; 0.6g of sodium chloride; 0.6g of calcium carbonate; the pH was adjusted to 7.0.
The components of the treated sewage were measured, and the results are shown in Table 5.
TABLE 5 results of measurement of various components in the treated water in comparative example 1 (unit: mg/L)
| Item | COD | Total nitrogen | Total phosphorus | Ammonia nitrogen | Nitro nitrogen |
| Numerical value | 121 | 137 | 9.2 | 53 | 0.8 |
As can be seen from the detection results of the treated water in Table 5, the combined use of the compound microbial inoculant and the unmodified calcium peroxide has a slow removal rate for organic pollutants and nitrogen and phosphorus in the sewage, and the removal rates for COD, total nitrogen, total phosphorus, ammonia nitrogen and nitrate nitrogen are respectively 48.3%, 75.2%, 78.6%, 77.0% and 61.8%.
The above description describes a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention as claimed. Any modification, equivalent replacement and improvement without departing from the principle and spirit of the present invention shall be considered to be within the protection scope of the present claims.
Claims (10)
1. A method for treating septic tank sewage by coupling compound microbial inoculant and calcium peroxide is characterized by comprising the following steps:
(1) adding modified calcium peroxide: adding modified calcium peroxide into the pretreated sewage;
(2) culturing a compound microbial agent: after the dissolved oxygen in the water body is stable, adding the compound microbial inoculum into the sewage, controlling the pH value of the sewage to be 7.0-8.0, the temperature to be 28-32 ℃ and the dissolved oxygen to be 3-5 mg/L, and culturing the compound microbial inoculum for 2-3 d;
(3) domesticating a compound microbial agent: adjusting the concentration of thallus in the sewage, controlling the pH value of the sewage to be 7.0-8.0 and the treated water amount to be 40-100 m3(ii) water temperature of 28-32 ℃, dissolved oxygen of 3-5 mg/L, domesticationThe compound microbial agent is prepared for 7-15 days;
(4) treating sewage: controlling the pH value of the sewage to be 7.0-8.0, the water temperature to be 28-32 ℃, the dissolved oxygen to be 3-5 mg/L and the hydraulic retention time to be 8-24 h, thus finishing the treatment of the sewage;
wherein the compound microbial agent consists of aerobic denitrifying bacteria, lactobacillus acidophilus, bacillus subtilis and actinomycetes.
2. The method for treating septic tank sewage by coupling the compound microbial agent and the calcium peroxide according to claim 1, wherein the preparation of the modified calcium peroxide comprises the following steps:
(11) CaO is weighed according to the proportion2Mixing the clean water sludge and the cement uniformly;
(12) preparing the mixture uniformly mixed in the step (11) into a sphere with the diameter of 0.5-1 cm;
(13) and (3) drying the spheres prepared in the step (12) at the temperature of 80-110 ℃ for 1-2 h to obtain the modified calcium peroxide.
3. The method for treating septic tank sewage by coupling the compound microbial inoculant and the calcium peroxide according to claim 2, wherein CaO is used for treating the septic tank sewage2The mass ratio of the water purification sludge to the cement is 0.8-1.7: 1: 0.56 to 1.
4. The method for treating septic tank sewage by coupling the compound microbial agent with the calcium peroxide as claimed in claim 1, wherein the preparation of the compound microbial agent comprises the following steps: sequentially inoculating aerobic denitrifying bacteria seed liquid, lactobacillus acidophilus seed liquid, bacillus subtilis seed liquid and actinomycetes seed liquid into a fermentation culture medium, performing fermentation culture for 36-48 h under the conditions that the pH is 7.0-8.2 and the temperature is 28-30 ℃ to obtain fermentation liquid, and drying the fermentation liquid to obtain the compound microbial agent.
5. The method for treating septic tank sewage by coupling the composite microbial inoculant and the calcium peroxide according to any one of claims 1 to 4, wherein the addition amount of the modified calcium peroxide in the step (1) is 0.5 to 0.8 per mill of the sewage volume.
6. The method for treating septic tank sewage by coupling the compound microbial inoculant and the calcium peroxide according to any one of claims 1 to 5, wherein the adding amount of the compound microbial inoculant in the step (2) is 0.4 to 0.6g/m3。
7. The method for treating septic tank sewage by coupling the composite microbial agent and the calcium peroxide according to claim 6, wherein the concentration of the bacteria in the step (3) is 1 x 120-6 x 120 bacteria/mL.
8. The method for treating septic tank sewage by coupling the composite microbial agent and the calcium peroxide according to claim 4, wherein the aerobic denitrifying bacteria seed solution, the lactobacillus acidophilus seed solution, the bacillus subtilis seed solution and the actinomycete seed solution account for 25-30%, 3-5%, 5-8% and 15-20% by volume respectively.
9. The method for treating septic tank sewage by coupling the composite microbial agent and the calcium peroxide, according to claim 4, is characterized by comprising the following components in percentage by weight:
the pH value of the fermentation medium is 7.0.
10. The method for treating septic tank sewage by coupling the composite microbial inoculant and the calcium peroxide according to any one of claims 1 to 9, wherein the pretreated sewage is septic tank sewage and comprises the following components in percentage by weight:
COD：100～300mg/L；
ammonia nitrogen: 200-400 mg/L;
total nitrogen: 300-500 mg/L;
total phosphorus: 20-50 mg/L.
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