CN115353211B - Application of bacillus megatherium LZP03 in treatment of pig raising wastewater - Google Patents
Application of bacillus megatherium LZP03 in treatment of pig raising wastewater Download PDFInfo
- Publication number
- CN115353211B CN115353211B CN202210107973.XA CN202210107973A CN115353211B CN 115353211 B CN115353211 B CN 115353211B CN 202210107973 A CN202210107973 A CN 202210107973A CN 115353211 B CN115353211 B CN 115353211B
- Authority
- CN
- China
- Prior art keywords
- pig raising
- wastewater
- raising wastewater
- waste water
- lzp03
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002351 wastewater Substances 0.000 title claims abstract description 192
- 241000194107 Bacillus megaterium Species 0.000 title claims abstract description 23
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 98
- 239000001963 growth medium Substances 0.000 claims abstract description 54
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 49
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 26
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 26
- 239000011574 phosphorus Substances 0.000 claims abstract description 26
- 239000003344 environmental pollutant Substances 0.000 claims abstract description 18
- 231100000719 pollutant Toxicity 0.000 claims abstract description 17
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 241000282898 Sus scrofa Species 0.000 claims description 182
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 64
- 229910052799 carbon Inorganic materials 0.000 claims description 64
- 238000000855 fermentation Methods 0.000 claims description 53
- 230000004151 fermentation Effects 0.000 claims description 53
- 238000000034 method Methods 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 22
- 239000001888 Peptone Substances 0.000 claims description 11
- 108010080698 Peptones Proteins 0.000 claims description 11
- 235000015278 beef Nutrition 0.000 claims description 11
- 235000019319 peptone Nutrition 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 4
- 239000002054 inoculum Substances 0.000 claims description 4
- 239000008223 sterile water Substances 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 238000002203 pretreatment Methods 0.000 claims 3
- 244000005700 microbiome Species 0.000 abstract description 32
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 abstract description 20
- 230000009286 beneficial effect Effects 0.000 abstract description 19
- 239000002028 Biomass Substances 0.000 abstract description 15
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 238000003912 environmental pollution Methods 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 5
- 238000006731 degradation reaction Methods 0.000 abstract description 5
- 238000004064 recycling Methods 0.000 abstract description 5
- 241001052560 Thallis Species 0.000 description 16
- 230000012010 growth Effects 0.000 description 14
- 238000012216 screening Methods 0.000 description 13
- 239000002609 medium Substances 0.000 description 11
- 230000009467 reduction Effects 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- 230000008859 change Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 8
- 239000002921 fermentation waste Substances 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 5
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000005202 decontamination Methods 0.000 description 4
- 230000003588 decontaminative effect Effects 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002068 microbial inoculum Substances 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000011197 physicochemical method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000006004 Quartz sand Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000282894 Sus scrofa domesticus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000004103 aerobic respiration Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000014075 nitrogen utilization Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- -1 organic matters Chemical compound 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/22—Nature of the water, waste water, sewage or sludge to be treated from the processing of animals, e.g. poultry, fish, or parts thereof
Abstract
The invention discloses application of bacillus megatherium LZP03 in treating pig raising wastewater. The bacillus megatherium LZP03 is preserved in China Center for Type Culture Collection (CCTCC) with a strain preservation number of CCTCC NO. M2018599. According to the invention, different kinds of beneficial microorganisms are inoculated into pig raising wastewater to screen and obtain bacillus megatherium LZP03 which grows in the pig raising wastewater and has a relatively high propagation speed, and the utilization condition of nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, so that LZP03 can grow in an original pig raising wastewater culture medium and has relatively high biomass, and the biomass is remarkably improved after the pig raising wastewater culture medium with optimized carbon nitrogen ratio is inoculated into LZP 03. The LZP strain is fermented in the pig raising wastewater culture medium with optimized carbon-nitrogen ratio, has good removal rate for COD, ammoniacal nitrogen and phosphorus, reduces the possibility of environmental pollution caused by the pig raising wastewater on one hand, and is more beneficial to the recycling of the pig raising wastewater on the other hand.
Description
Technical Field
The invention relates to a microorganism strain for treating pig raising wastewater, in particular to application of bacillus megaterium LZP03 in treating pig raising wastewater, and also relates to a method for treating pig raising wastewater. The invention belongs to the technical field of agricultural production.
Background
China is one of the most huge industrial countries for large-scale pig breeding and consumption in the world. Along with the continuous development of economy, the scale of a live pig breeding industry chain is continuously expanded, according to the year of statistics in 2016, the annual pork yield in 2015 of China is 5486.5 ten thousand tons, the live pig breeding scale is huge (national statistical bureau, 2016), and the produced livestock manure also becomes a major pollution source, so that the problem of environmental pollution is also more and more remarkable. The total production of livestock manure in China reaches 2.43x10 8 Ton of urine 1.63x10 8 Ton, total nitrogen 1.02x10 6 Ton, total phosphorus 1.60x10 5 Ton (Pan Qing, 2002), COD discharge amount reaches 7118 ten thousand tons, which is far more than the sum of the discharge amounts of industrial wastewater and domestic wastewater COD, and most of pig farms use flushing water to clean pig houses, so pig farm wastewater, such as pig urine, excrement and the like, are mixed with each other, ammonia nitrogen content, phosphorus content, suspended matters and organic matters in the wastewater are high in concentration and seriously unbalanced in carbon nitrogen ratio, and if a large amount of untreated discharge is discharged to the environment such as lakes, rivers, farms and the like, the water body is enriched, soil properties are changed and other column pollution problems are caused.
At present, the pig raising wastewater treatment technology at home and abroad mainly focuses on three major modes, namely a physical and chemical treatment technology, a natural treatment technology and a biological treatment technology. Among the methods used in the physicochemical treatment are a medium adsorption method, a flocculation precipitation method and the like, such as Qian Feng, the zeolite-straw combination is used for filtering the swine waste water, and the removal rates of COD, ammoniacal nitrogen and phosphorus can reach 47.9%, 72.9% and 50.1% (Qian Feng, 2008) respectively, and 61.02% of swine waste water with the COD concentration of 3232mg/L can be removed by a magnetic flocculation method like Cui Lina and the like (Cui Lina, 2010). Although the physicochemical method has better pollutant removal rate on the swine waste water, the physicochemical method has the disadvantages of lower broad spectrum, higher pollutant removal cost, larger investment of treatment equipment, less engineering application and the like on the swine waste water pollution removal. The natural treatment method generally utilizes the comprehensive action of natural soil, water body and organisms to remove pollutants, such as Zhu Xizhen, and the like, and the artificial wetland construction is carried out through blast furnace slag, quartz sand and other matrixes, so that the removal rates of COD, BOD and phosphorus respectively reach 71% -88%, 80% -89%, 70% -85% (Zhu Xizhen, 2003), and Lu Xiuguo and the like, and the pig raising wastewater COD is less than or equal to 400mg/L and ammonia nitrogen is less than or equal to 70mg/L (Lu Xiuguo, 2009) by utilizing the oxidation pond. The natural treatment method has better decontamination capability on polluted wastewater, but has insufficient stability and longer purification time. The biological treatment technology is a novel method for treating high-concentration organic wastewater by using the catalysis of microorganisms, such as Liang Meidong, and the like, which uses an SRB reactor to treat the pig raising wastewater by aeration can increase the removal rate of COD to more than 90% (Liang Meidong, 2009), and Li Fengmin, and the like, can enable the removal rate of ammoniacal nitrogen and total nitrogen to reach 99.7% and 50.7% by adopting an aerobic-anaerobic combined treatment method (Li Fengmin, 2011). However, a large amount of activated sludge is generated in the process of treating the wastewater by a biological treatment method, and cannot be treated, so that secondary pollution is caused to the environment.
However, although the pig raising waste water causes a series of pollution to the environment, the pig raising waste water cannot be denied that the pig raising waste water contains a great amount of organic matters, nitrogen, phosphorus, potassium and other nutrient elements, and the elements are also necessary for the growth of microorganisms, so that the pig raising waste water has positive significance for improving the quantity and quality of microorganisms. If the pig raising waste water is used as a basic culture medium for fermentation production of beneficial microorganisms, the production cost of the microbial fertilizer can be greatly reduced, and the pollution problem can be reduced through biodegradation, so that the waste water is changed into valuables, the residual value of the waste water is realized, a large amount of resources for treating environmental pollution can be saved, and the method has very important significance for the promotion of harmless resource utilization of environmental pollutants.
Therefore, the invention takes the pig raising wastewater as a natural screening culture medium, screens different kinds of beneficial microorganism strains to obtain strains which grow vigorously and have a faster propagation speed in the pig raising wastewater, optimizes the nutrient content of the pig raising wastewater culture medium to improve the biomass of the strains, detects the utilization condition of the nutrient content of the pig raising wastewater by the strains after optimization and the removal effect of pollutants, explores the fermentation system and the process of the pig raising wastewater, and provides technical support for harmless resource utilization of the pig raising wastewater.
Disclosure of Invention
One of the objects of the present invention is to provide a microorganism strain useful for treating swine wastewater;
the second object of the invention is to provide a method for treating pig raising wastewater.
In order to achieve the above purpose, the invention adopts the following technical means:
according to the invention, different kinds of beneficial microorganisms are inoculated into pig raising wastewater to screen and obtain bacillus megaterium (Bacillus megaterium) LZP (CCTCC NO: M2018599) growing in the pig raising wastewater and having a relatively high breeding speed, and the utilization condition of nutrient components in the pig raising wastewater and the degradation condition of pollutants in the pig raising wastewater are detected, so that LZP03 can grow in the original pig raising wastewater and has relatively high biomass, and the biomass in the pig raising wastewater with optimized carbon nitrogen ratio is improved more remarkably, wherein the highest viable count of LZP03 is 4.26 multiplied by 10 10 cfu/mL, and compared with LZP strain which utilizes carbon, nitrogen and organic carbon in the original and optimized pig raising wastewater, the situation that each strain is metabolized more vigorously in the optimized culture medium is proved, LZP strain is fermented in the pig raising wastewater culture medium with optimized carbon nitrogen ratio, the COD removal rate is 92%, the ammonia nitrogen removal rate is 90.35%, the phosphorus removal rate is 69.10%, the pH value of the original pig raising wastewater can be effectively regulated, the possibility of environmental pollution caused by fermentation waste liquid is reduced, and the recycling utilization of the pig raising wastewater is better promoted.
Based on the research, the invention firstly provides the application of the bacillus megatherium (Bacillus megaterium) LZP03 in the treatment of the pig raising wastewater, wherein the bacillus megatherium LZP is named Bacillus megaterium LZP03, the classification names are Bacillus megaterium LZP03 respectively, the bacillus megatherium is preserved in China center for type culture collection, the address is in university of Wuhan, china, the strain preservation number is CCTCC NO. M2018599, and the preservation time is 2018, 9 and 6.
Preferably, the seed solution of bacillus megaterium LZP03 is inoculated into sterilized pig raising waste water to be treated, and the treated pig raising waste water is obtained after fermentation, and the treated pig raising waste water has lower pollutant content compared with the treated pig raising waste water.
Wherein, preferably, the method further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
wherein, preferably, the fermentation is carried out at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig raising wastewater is reduced compared with the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Further, the invention also provides a method for treating the pig raising wastewater, which comprises the following steps:
(1) Inoculating Bacillus megaterium LZP03 into beef extract peptone culture medium for activation, multiplexing activated strain into beef extract peptone culture medium, and adjusting strain concentration to OD with sterile water 600 =1.0 as seed fluid; the bacillus megatherium LZP03 is preserved in China Center for Type Culture Collection (CCTCC) with a strain preservation number of CCTCC NO. M2018599;
(2) Inoculating the seed liquid into sterilized pig raising waste water to be treated, and fermenting to obtain treated pig raising waste water, wherein the treated pig raising waste water has lower pollutant content than the treated pig raising waste water.
Wherein, preferably, the step (2) further comprises the step of adding brown sugar into the sterilized pig raising wastewater to be treated, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
wherein, in the step (2), seed liquid is inoculated into sterilized pig raising wastewater to be treated according to the inoculum size of 1-2 vol%.
Wherein, preferably, the fermentation in the step (2) means fermentation culture at 30 ℃ and 120r/min for 24-96h.
Preferably, the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in the treated pig raising wastewater is reduced compared with the content of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus before treatment.
Compared with the prior art, the invention has the beneficial effects that:
the invention realizes the recycling utilization of pig raising wastewater by using beneficial microorganisms, the technology is also based on a biological treatment method, and the core of the technology is to remove pollutants from pig raising wastewater by using a microorganism aerobic-anaerobic combined treatment method, and the invention creatively utilizes rich nutrition in pig raising wastewater to realize mass propagation of beneficial microorganisms so as to obtain beneficial microorganism thalli for preparing microbial agents, and creates economic value while removing the pollutants to protect the environment. The invention takes pig raising waste water as a natural culture medium to culture microorganisms, so the pig raising waste water is selected because a large amount of organic matters, carbon sources, nitrogen sources and other nutrient substances contained in the pig raising waste water can provide sufficient nutrients for the growth of microorganisms, the natural temperature bed for the culture of microorganisms is adopted, and substances harmful to the environment in the pig raising waste water can be absorbed, converted and fixedly used for the proliferation of thalli and the secretion of metabolites in the microorganism growth process, so that on one hand, the pollution sources such as ammonia nitrogen, organic matters, phosphorus and the like which are caused to the environmental safety in the pig raising waste water are reduced, on the other hand, a large amount of microorganism thalli which are cultured by the pig raising waste water can be collected for various microbial preparations such as plant promotion, crop biocontrol, pollutant degradation and the like, the cost of the culture medium of microorganisms is high in the culture process, one of main reasons for causing the price of the microorganism microbial inoculum is high, and the use of the pig raising waste water is close to the beneficial microorganism strains in viable count to the traditional culture medium, but the cost is greatly reduced, and the green and the agricultural microbial inoculum is greatly reduced.
The invention optimizes and adjusts the carbon-nitrogen ratio of the pig raising wastewater, then inoculates beneficial microorganisms, and finally obtains 1 beneficial microorganism LZP03 with higher viable count through screening, and has excellent effect on the aspect of crop growth promotion, wherein the highest viable count of LZP03 can reach 4.26 multiplied by 10 10 cfu/mL, then centrifugally collecting thalli, and then detecting fermentation waste liquid, wherein the removal rate of the waste liquid on the waste water pollutants of pig raising is found to be better, the removal rate of organic matters of LZP03 is 89.62%, the removal rate of COD is 92%, the removal rate of ammoniacal nitrogen is 90.35%, the removal rate of phosphorus content is 69.10%, andLZP03 can effectively reduce the pH of the original pig raising wastewater, so that the original alkaline solution is reduced to neutral or slightly acidic pH solution, and the salinization of the soil and the damage of the soil structure caused by filtering out the wastewater are prevented.
Therefore, the beneficial microorganism realizes the pig raising wastewater recycling utilization, the decontamination capability of the pig raising wastewater is not weaker than that of the main stream physicochemical treatment technology, the natural treatment technology and the traditional biological treatment technology at home and abroad, the effect is even better, and the technology has higher broad spectrum on the types of pollutant removal in the pig raising wastewater more comprehensively; the pig raising wastewater treatment by microbial fermentation is lower in decontamination cost, equipment investment is low, a large number of sites are not needed, decontamination period is short, small fermentation equipment can be quickly built near the source to treat the pig raising wastewater, and a small enterprise can also bear the system construction cost; the beneficial microorganism is used for fermentation, so that the beneficial microorganism strain can be obtained for the production and use of the microbial agent while the pig raising wastewater is treated, the damage of pollutants to the environment is reduced, the economic benefit is reported, the promotion of the microbial agent is promoted, the development of green agriculture is accelerated, and a virtuous circle system of ecological protection-agricultural development is developed, so that the sustainable development strategy of China is met.
Drawings
FIG. 1 shows the biomass of the LZP strain in swine wastewater after initial and optimized carbon-nitrogen ratios over time;
FIG. 2 shows the total nitrogen content change and the reduction rate (A) of the original (B) treated by LZP03 and the pig raising wastewater (C) after optimization;
FIG. 3 shows the total carbon content change and the reduction rate (A) of the original (B) treated by LZP03 and the optimized pig raising wastewater (C);
FIG. 4 shows the change in total organic carbon content and the rate of decrease (A) of the raw (B) treated by LZP03 and the optimized pig wastewater (C);
FIG. 5 is a graph showing a comparison of the reduction rate of total nitrogen, total carbon, and total organic carbon in the supernatant of LZP03 fermentation broth;
FIG. 6 shows the pH trend of LZP03 in optimized medium with fermentation time;
FIG. 7 shows the removal rate of COD in the fermentation broth of LZP03 and the trend with time;
FIG. 8 shows the removal rate of LZP03 for ammonia nitrogen and phosphorus content.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in connection with the embodiments of the present invention. It is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments, and that all other embodiments obtained by persons of ordinary skill in the art without making creative efforts based on the embodiments in the present invention are within the protection scope of the present invention.
Example 1 screening of Strain and its application in treatment of pig raising wastewater
1 materials and methods
1.1 physical and chemical Properties of raw pig raising wastewater
The pig raising wastewater used in the test is taken from a pig raising field in the zizichihal Fula base region, and the collected pig raising wastewater is stored at the temperature of 4 ℃ for later experiments, and the basic characteristics of the pig raising wastewater are shown in table 1.
TABLE 1 physicochemical Properties of pig raising wastewater
1.2 test Strain Source
The strains used in the test are all different pure culture beneficial microorganisms obtained by subject matter separation and screening, and researches find that each strain is a high-efficiency strain with better industrial application potential in the aspects of plant growth promotion, biological control, pollutant control and the like, and the strains are preserved in a refrigerator at the temperature of minus 80 ℃ for standby, and all strains are identified by strain morphology and 16SrDNA classification, and an identification list is shown in a table 2.
TABLE 2 test strains
1.3 Medium
Beef extract peptone medium: 10.00g of peptone, 3.00g of beef extract, 5.00g of NaCl, 20.00g of agar, and distilled water with a constant volume of 1000.00mL, wherein agar is not added into the liquid culture medium.
Original pig raising wastewater culture medium: pig raising waste water from pig farm transportation.
Pig raising wastewater culture medium with optimized carbon-nitrogen ratio: 10g of brown sugar, and the volume of the pig raising wastewater is fixed to 1000.00mL, so that the carbon-nitrogen ratio of the pig raising wastewater is 16-20:1.
1.4 high-efficient degradation Strain preliminary screening of pig raising wastewater
1.4.1 seed liquid preparation
The test strains in Table 2 were inoculated into beef extract peptone medium, and cultured at 30℃for 24 hours for activation. The activated strains were multiplexed in a beef extract peptone medium, shake-cultured at 30℃and 120r/min, and each strain cultured to the same concentration (OD 600 = 1.0) was used as a seed solution for a treatment test of swine wastewater.
1.4.2 Strain Primary screening
In order to screen strains capable of growing in a large amount in the pig raising wastewater, seed solutions of different strains are respectively inoculated into a triangular flask filled with 100mL of sterilized pig raising wastewater according to an inoculum size of 1vol%, and the strains are subjected to shaking culture at 30 ℃ and 120r/min, fermentation liquor is sampled every 24 hours, and the number of viable bacteria in each time period is detected to determine the maximum viable bacteria amount of the strains. The number of viable bacteria is determined by a dilution coating method, 10mL of the fermented culture medium is taken and placed into a 90mL triangular flask with glass beads and sterile water, gradient dilution is carried out after shaking for 30min in a shaking table of 200r/min, 0.1mL of dilution liquid with proper dilution concentration is selected to be coated on a solid plate of beef extract peptone culture medium, culture is carried out for 24h at 30 ℃, the number of viable bacteria (cfu/mL) is calculated, and three groups of the culture medium are arranged in parallel. And then selecting strains with higher fermentation biomass for subsequent experiments.
1.5 exploration of the effect of screening strains on the utilization of pig raising wastewater
1.5.1 variation of the growth conditions and the treatment effects of the screened strains in the raw wastewater and the optimized wastewater with time based on the carbon-nitrogen ratio
Because the carbon-nitrogen ratio value in the pig raising wastewater stock solution is smaller, the growth and propagation of thalli can be inhibited, and the treatment effect of the pig raising wastewater is reduced, so that the pig raising wastewater culture medium is subjected to proper carbon-nitrogen ratio optimization, whether the thalli content can be improved is detected, and the treatment capacity of the pig raising wastewater is enhanced. The dominant strains obtained after screening are respectively inoculated into a beef extract peptone culture medium to prepare seed liquid, the seed liquid is respectively inoculated into 300mL of an original pig raising wastewater culture medium and an optimized culture medium added with 1wt% of brown sugar according to the inoculum size of 2vol%, the culture medium is subjected to shake flask culture at 30 ℃ and 120r/min, and samples are sampled and measured for each index every 24 hours.
1.5.2 treatment effect of optimized Carnis Sus Domestica wastewater treated by screening strains after centrifugation
In order to simulate whether the pig raising waste liquid treated by various strains after the thalli are acquired in the industrial production process can further reduce the potential of environmental threat, the experiment carries out index detection after centrifuging sample liquids with different fermentation time. Inoculating seed solution of the screened strain into an optimized culture medium, culturing at 30 ℃ under 120r/min shaking, sampling samples every 24h, centrifuging for 5min under the condition of 12000rpm, and measuring indexes.
1.6 test measurement index
1.6.1 Water quality index
And (3) measuring COD, pH, total nitrogen, total carbon, total organic carbon, ammoniacal nitrogen and phosphorus content of the sample.
1.6.2 microbial indicators
10mL of fermentation broth is put into a shaking table with 90mL of sterile water at 200r/min to shake for 30min, and then gradient dilution is carried out to obtain 10 -7 、10 -8 、10 -9 And (3) taking 0.1mL of the diluted solution, coating the diluted solution on a beef extract peptone culture medium plate, culturing at 30 ℃ for 24 hours, and calculating the viable count (cfu/mL) to form three groups of parallel groups.
2. Results and discussion
2.1 pig wastewater treatment Strain screening
The aim of screening different strains is achieved by detecting the maximum content of the viable count of each strain in the pig raising wastewater, and the pig raising wastewater is usedCulturing each beneficial strain to find LZP03 can grow in pig raising waste water and has high biomass, and the maximum value of the viable count in the pig raising waste water is LZP 03=1.76×10 10 cfu/mL was higher than the live bacteria of other strains for subsequent experiments.
2.2LZP03 compared with the growth condition of thalli in the original and optimized pig raising wastewater culture medium along with time
To determine the biomass of LZP strain in different media, viable count determination was performed by gradient dilution plating on the original and optimized media, respectively. As can be seen from FIG. 1, the maximum viable count of LZP03 reaches 1.76X10 after 72 hours of cultivation in the original pig raising wastewater medium 10 cfu/mL, the maximum viable count of 4.26 multiplied by 10 is achieved in the optimized pig raising wastewater culture medium for 96 hours 10 cfu/mL, 56.86% biomass is improved, so that the biomass of each strain in the pig raising wastewater can be obviously improved by adding brown sugar for carbon-nitrogen ratio optimization, the processing capacity of thalli on the pig raising wastewater is improved, and the thalli acquisition of beneficial microorganisms can be better improved.
2.3LZP03 changes of total nitrogen, total carbon and total organic carbon of fermentation liquor in the growth process of original and optimized pig raising wastewater culture medium
2.3.1LZP03 Total Nitrogen modification in raw and optimized pig raising wastewater Medium
LZP03 is inoculated to an original pig raising wastewater culture medium and an optimized pig raising wastewater culture medium respectively, and the total nitrogen of the fermentation liquid is measured every 24 hours. As can be seen from fig. 2, the total nitrogen content of the fermentation broth after the original pig raising wastewater was inoculated with LZP strain generally showed a continuous decrease trend with the increase of the strain fermentation time, and the total nitrogen content of the LZP fermentation broth was reduced to a minimum value of 178.4mg/L at 96h of cultivation, compared with the control group, by 55.75%. The LZP fermentation liquor using the optimized pig raising wastewater culture medium also shows the overall decreasing trend along with the prolongation of the fermentation time, the total nitrogen content rapidly decreases in the fermentation interval of 0-24h, then the total nitrogen content gradually becomes gentle, and the total nitrogen content reaches the minimum value of 104.8mg/L respectively when LZP is cultured for 96h, compared with the total nitrogen content of LZP03 of the control group, the total nitrogen content decreases by 68.62 percent. According to comparison, LZP03 can be found that the reduction rate and the reduction speed of total nitrogen in fermentation liquor grown by the original pig raising wastewater are smaller than those of fermentation liquor inoculated with pig raising wastewater with optimized carbon nitrogen ratio, and the nitrogen overflow in a culture medium possibly caused by denitrification, so that the nitrogen utilization of a strain can be better improved after the carbon nitrogen ratio is optimized.
2.3.2 comparing the total carbon treatment conditions in the original and optimized pig raising wastewater culture media
And (3) respectively inoculating the screened strain to an original pig raising wastewater culture medium and an optimized pig raising wastewater culture medium, and measuring total carbon of the fermentation liquor every 24 hours. As shown in fig. 3, after the LZP strain is inoculated into the original pig raising wastewater medium, the total carbon content in the fermentation broth shows a tendency of slowly decreasing and then stabilizing along with the increase of the culture time, the minimum value of the total carbon content of the LZP strain 03 is 740.0mg/L, and compared with the control group, the total carbon reduction rate of the fermentation broth of the screening strain LZP03 is 24.61%. After the screened strain is inoculated to the optimized pig raising waste water culture medium, the total carbon content in the fermentation broth is continuously reduced along with the improvement of the culture time, the speed is higher, the fermentation broth finally tends to be slowly reduced, the total carbon content of each strain fermentation broth reaches the minimum value LZP03 =1722 mg/L after 96 hours of culture, and the total carbon reduction rate is reduced by 66.60 percent compared with a control group. LZP03 is cultured in the optimized culture medium, the total carbon content reduction rate in the fermentation broth is remarkably improved compared with that in the original pig raising wastewater, and the reduction of the total carbon content is probably caused by the fact that microorganisms continuously perform aerobic respiration to discharge carbon dioxide in the growth process to reduce the total carbon content, which indicates that the microorganisms grow and metabolize more actively in the optimized culture medium, so that the optimized pig raising wastewater culture medium can well promote the growth of strains and improve the metabolic capability of the strains.
2.3.3 comparison of the treatment conditions of the total organic carbon in the original and optimized pig raising wastewater fermentation solutions
As can be seen from fig. 4, after the strain LZP03 is inoculated, the total organic carbon content in the original pig raising wastewater fermentation broth shows a tendency of increasing and then decreasing with the increase of the culture time of the thalli, compared with the control group, the minimum total organic carbon content after LZP fermentation is 294.8mg/L, the total organic carbon reduction rate is 18.54%, while LZP03 is inoculated in the optimized pig raising wastewater culture medium, the minimum total organic carbon content of the fermentation broth reaches 70.56% with the increase of the culture time compared with the control group LZP03, and the optimized pig raising wastewater culture medium has a more rapid consumption and consumption rate of the total organic carbon compared with the original pig raising wastewater culture medium. The reduction after the increase of the total organic carbon in the fermentation broth of the strain cultured by the original pig raising wastewater is probably due to the fact that microorganisms degrade some insoluble solid pig manure and impurities in the pig raising wastewater, the degradation speed of thalli is higher than the total organic carbon consumed by the thalli for self synthesis or metabolism in the early stage, so that the total organic carbon is continuously accumulated, the total organic carbon consumption is continuously increased along with the continuous increase of the number of the strain, and the substrate amount is continuously consumed, so that the total organic carbon accumulation speed in the fermentation broth is lower than the consumption speed, and the total organic carbon content is continuously reduced. The continuous decrease in total organic carbon consumption of the optimized medium may be due to the fact that the total organic carbon content of the thalli, which quickly enter the growth log phase in the early stage after carbon source supplementation, is greatly increased to be used for self synthesis and metabolism, and is far greater than the accumulation amount, so that the total organic carbon content is continuously decreased.
2.4 Change of various indicators of fermentation waste liquid after removal of thalli from pig raising waste water treated with LZP03
The change of biomass, total nitrogen, total carbon and total organic carbon in the original and optimized pig raising waste water culture medium growth process of LZP is compared, and the strain can better grow and reproduce the biomass in the optimized pig raising waste water culture medium, and has remarkable improvement and stronger self-capacity for metabolism and utilization of nutrients in pig raising waste water, so that the optimized pig raising waste water culture medium is selected as a basic culture medium for industrial fermentation of beneficial microorganisms, and meanwhile, the potential threat of filtered fermentation waste liquid to the environment can be better reduced after LZP03 is fermented in the pig raising waste water and the thalli are harvested in the industrial production process is simulated, so that the strain fermentation liquid is removed by a high-speed centrifugation method, and the change condition of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen and phosphorus in supernatant fluid is measured.
2.4.1LZP03 variation of total nitrogen, total carbon and total organic carbon content in optimized pig raising waste water culture medium
LZP03 was inoculated into an optimized swine waste water medium and the centrifuged supernatant was assayed for total nitrogen, total carbon and total organic carbon every 24 hours. As shown in FIG. 5, compared with the control group, the total nitrogen content in the supernatant of the LZP03 fermentation broth after centrifugation is reduced by 94.58%, the total carbon content is reduced by 85.96%, and the total organic carbon content is reduced by 89.62%, which indicates that LZP strain 03 can better utilize the nutritional ingredients in the pig raising wastewater to perform self growth, propagation and metabolism activities, and prevent the filtered fermentation wastewater from being rich in nutrients to cause environmental pollution.
pH change condition of 2.4.2LZP03 in optimized pig raising wastewater culture medium
The pH measurement was performed every 24 hours for each strain fermentation broth, and it is known from fig. 6 that after the pig raising wastewater is fermented for 96 hours by LZP strain, the pH of the pig raising wastewater is reduced from the original alkaline solution with ph=8.85 to the neutral fermentation broth with ph=7.34, which is probably because the strain secretes a certain amount of organic acid when growing itself in the alkaline pig raising wastewater by utilizing the nutrients of the pig raising wastewater during the fermentation process, thereby reducing the pH of the high alkaline pig raising wastewater, which is significant for preventing the filtered wastewater from causing soil salinization and damaging the soil structure.
2.4.3LZP03 COD content change and removal rate in optimized pig raising wastewater culture medium
In order to determine the COD removal effect of the screening strain on the pig raising wastewater, the COD detection is carried out on the supernatant of the LZP03 fermentation broth, as shown in fig. 7, along with the continuous increase of the fermentation time of the strain, the COD content of the strain is continuously and rapidly reduced from the original COD=16000 mg/L to the maximum COD=1280 mg/L, the COD removal rate reaches LZP03 =92%, which indicates that LZP03 has better removal rate on COD, the threat of filtered waste liquid to the environment is reduced, the strain is found to have small increase in COD content in a time interval of 72-96 h in a trend chart of the change of COD of the LZP03 fermentation broth, and the COD content is possibly reduced because the newly increased number of bacterial cells is less than the removal COD capability of the subsidence number in the stationary period and the COD concentration is increased to a certain extent due to the broken intracellular material flow of dead bacterial cells.
2.4.4LZP03 removal rate of ammoniacal nitrogen and phosphorus in optimized pig wastewater
As shown in FIG. 8, the LZP strain can greatly reduce the content of ammonia nitrogen in the fermentation waste liquid, the removal rate of the ammonia nitrogen reaches 90.35%, and the removal effect of the ammonia nitrogen is relatively good. The determination of the phosphorus content of the supernatant fluid of the fermentation liquid after the fermentation of the strain can be used for judging whether the strain can well reduce the phosphorus content in the fermentation waste liquid. Compared with the control group LZP, the removal rate of phosphorus can reach 69.10%, which shows that the phosphorus removal agent has certain phosphorus removal capability.
Conclusion 3
(1) LZP03 can grow in the original pig raising waste water and has higher biomass, while the biomass in the pig raising waste water with optimized carbon-nitrogen ratio is improved more obviously, wherein the highest viable count of LZP is 4.26 multiplied by 10 10 cfu/mL, and the situation that the strain LZP03 utilizes carbon, nitrogen and organic carbon in the original and optimized pig raising wastewater proves that each strain is more vigorous in metabolism in an optimized culture medium, so that the pig raising wastewater with optimized carbon nitrogen ratio is more suitable for strain growth and reproduction and resource utilization.
(2) LZP03 strain ferments in the pig raising waste water culture medium of optimizing carbon nitrogen ratio and has better removal rate to COD, ammoniacal nitrogen and phosphorus content, the removal rate of COD reaches 92%, the removal rate of ammoniacal nitrogen is 90.35%, the removal rate of phosphorus is 69.10%, and the pH value of the original pig raising waste water can be effectively regulated, thereby reducing the possibility of environmental pollution caused by fermentation waste liquid and better promoting the recycling utilization of pig raising waste water.
Claims (10)
1. The application of the bacillus megatherium (Bacillus megaterium) LZP03 in the treatment of pig raising wastewater, wherein the bacillus megatherium LZP is preserved in China center for type culture collection, and the strain preservation number is CCTCC NO. M2018599.
2. The use according to claim 1, wherein the seed liquid of bacillus megaterium LZP is inoculated into sterilized swine waste water to be treated, and the treated swine waste water is obtained after fermentation, wherein the treated swine waste water has a reduced pollutant content compared to the pre-treatment swine waste water.
3. The use according to claim 1, further comprising the step of adding brown sugar to the sterilized swine waste water to be treated such that the swine waste water has a carbon to nitrogen ratio of 16-20:1.
4. the use according to claim 2, wherein the fermentation is carried out at 30℃and 120r/min for 24-96 hours.
5. The use according to claim 3, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus as compared to the pre-treatment swine wastewater.
6. A method for treating pig raising wastewater, comprising the steps of:
(1) Inoculating Bacillus megaterium LZP03 into beef extract peptone culture medium for activation, multiplexing activated strain into beef extract peptone culture medium, and adjusting strain concentration to OD with sterile water 600 =1.0 as seed fluid; the bacillus megatherium LZP03 is preserved in China Center for Type Culture Collection (CCTCC) with a strain preservation number of CCTCC NO. M2018599;
(2) Inoculating the seed liquid into sterilized pig raising waste water to be treated, and fermenting to obtain treated pig raising waste water, wherein the treated pig raising waste water has lower pollutant content than the treated pig raising waste water.
7. The method of claim 6, wherein the step (2) further comprises the step of adding brown sugar to the sterilized swine wastewater to be treated such that the swine wastewater has a carbon to nitrogen ratio of 16-20:1.
8. the method according to claim 6, wherein in the step (2), the seed solution is inoculated in an inoculum size of 1 to 2 vol.% into the sterilized swine wastewater to be treated.
9. The method according to claim 6, wherein the fermentation in the step (2) is performed at 30℃and 120r/min for 24-96 hours.
10. The method of claim 6, wherein the treated swine wastewater has reduced levels of total nitrogen, total carbon, total organic carbon, COD, ammoniacal nitrogen, phosphorus as compared to the pre-treatment swine wastewater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210107973.XA CN115353211B (en) | 2022-01-28 | 2022-01-28 | Application of bacillus megatherium LZP03 in treatment of pig raising wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210107973.XA CN115353211B (en) | 2022-01-28 | 2022-01-28 | Application of bacillus megatherium LZP03 in treatment of pig raising wastewater |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115353211A CN115353211A (en) | 2022-11-18 |
| CN115353211B true CN115353211B (en) | 2023-11-17 |
Family
ID=84030188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210107973.XA Active CN115353211B (en) | 2022-01-28 | 2022-01-28 | Application of bacillus megatherium LZP03 in treatment of pig raising wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115353211B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115287209B (en) * | 2022-01-28 | 2023-07-07 | 齐齐哈尔大学 | Composite microbial agent and application thereof in treating swine waste water |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164388A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝生物集团有限公司 | Bacillus megaterium and application thereof in aquaculture |
| CN105779322A (en) * | 2014-12-23 | 2016-07-20 | 北京大北农科技集团股份有限公司 | Bacillus subtilis strain, microecological preparation, and applications thereof |
| CN107586745A (en) * | 2017-10-20 | 2018-01-16 | 湖南省微生物研究院 | A kind of livestock excrement composting deodorizing and nitrogen protecting bacterial strain, microbial inoculum and its preparation method and application |
| CN109943501A (en) * | 2019-03-01 | 2019-06-28 | 中国水产科学研究院珠江水产研究所 | One plant of bacillus megaterium P5-2 and its separation method and application |
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| WO2021041603A1 (en) * | 2019-08-27 | 2021-03-04 | Microbial Discovery Group, Llc | Microorganisms for treatment of waste, water, or soil or for feeding to animals |
| CN112877247A (en) * | 2021-02-24 | 2021-06-01 | 华南理工大学 | Method for treating high ammonia nitrogen wastewater by using bacillus |
-
2022
- 2022-01-28 CN CN202210107973.XA patent/CN115353211B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104164388A (en) * | 2014-07-09 | 2014-11-26 | 青岛蔚蓝生物集团有限公司 | Bacillus megaterium and application thereof in aquaculture |
| CN105779322A (en) * | 2014-12-23 | 2016-07-20 | 北京大北农科技集团股份有限公司 | Bacillus subtilis strain, microecological preparation, and applications thereof |
| CN107586745A (en) * | 2017-10-20 | 2018-01-16 | 湖南省微生物研究院 | A kind of livestock excrement composting deodorizing and nitrogen protecting bacterial strain, microbial inoculum and its preparation method and application |
| CN109943501A (en) * | 2019-03-01 | 2019-06-28 | 中国水产科学研究院珠江水产研究所 | One plant of bacillus megaterium P5-2 and its separation method and application |
| WO2021041603A1 (en) * | 2019-08-27 | 2021-03-04 | Microbial Discovery Group, Llc | Microorganisms for treatment of waste, water, or soil or for feeding to animals |
| CN111925964A (en) * | 2020-08-21 | 2020-11-13 | 扬州大学 | Bacillus megaterium and application thereof |
| CN112877247A (en) * | 2021-02-24 | 2021-06-01 | 华南理工大学 | Method for treating high ammonia nitrogen wastewater by using bacillus |
Non-Patent Citations (3)
| Title |
|---|
| "Screen, identification and analysis on the growth-promoting ability for the rice growth-promoting rhizobacteria";Liu ZePing et al.;《Journal of Agricultural Resources and Environment 》;第35卷(第2期);第119-125页 * |
| "一株异养硝化巨大芽胞杆菌的分离鉴定及其脱氮性能研究";李忠徽等;《农业生物技术学报》;第27卷(第8期);第1331-1340页 * |
| "芽孢杆菌组合对海水养殖水体COD的降解效果";辛美丽等;《广西科学院学报》;第32卷(第3期);第215-220页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115353211A (en) | 2022-11-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105368745B (en) | Composite microbial preparation for treating black and odorous rivers and preparation method thereof | |
| CN109943497B (en) | Pseudomonas stutzeri with aerobic denitrification function and application thereof | |
| CN115353986B (en) | Bacillus bailii strain WB strain for treating pig raising wastewater and application thereof | |
| CN106906170A (en) | Complex micro organism fungicide and its preparation method and application | |
| CN105420147A (en) | Compound microbial preparation for treating black and odorous rivers through strengthened calcium nitrate | |
| CN103695317B (en) | There is the production method of the efficient phosphate-solubilizing penicillium oxalicum microbial inoculum of heavy metal tolerance characteristic | |
| CN104911130A (en) | Halomonas sp. with denitrogenation capability and application thereof | |
| CN103642703B (en) | There is the production method of the efficient phosphate-solubilizing aspergillus japonicus microbial inoculum of heavy metal tolerance characteristic | |
| CN104673724A (en) | Composite photosynthetic bacteria preparation applied to sewage treatment and preparation method of composite photosynthetic bacteria preparation | |
| CN104250625A (en) | Aerobic denitrifying bacterium strain and application thereof to water denitrification | |
| CN110699285B (en) | Palyalisma and application thereof in treating landfill leachate membrane concentrated solution | |
| CN110656071B (en) | Paracoccus huilkii for efficiently degrading DMF (dimethyl formamide) and application thereof | |
| CN115353211B (en) | Application of bacillus megatherium LZP03 in treatment of pig raising wastewater | |
| CN109081447B (en) | Method for removing nitrogen and phosphorus in culture wastewater by combining chlorella, acinetobacter and pseudomonas | |
| CN115353987B (en) | Bacillus subtilis strain SC strain for treating pig raising wastewater and application thereof | |
| CN115353210B (en) | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater | |
| CN107285482B (en) | Environment-friendly enzyme for purifying eutrophic water and preparation method thereof | |
| CN101701197A (en) | Novel microorganism flora mixture and mixed nutrient medium thereof | |
| CN111139198B (en) | Lactobacillus parvum GBW-HB1903 and application thereof | |
| CN110699287B (en) | Bacterial strain with function of degrading organic amine substances and application thereof | |
| CN111286478A (en) | Grease degradation composite bacterial agent and preparation method and application thereof | |
| CN110699313A (en) | Screening and culturing method of specific microbial flora for degrading industrial wastewater | |
| CN115287208A (en) | Method for producing beneficial microbial agent by using pig raising wastewater while removing environmental pollutants in pig raising wastewater | |
| CN115612647A (en) | Preparation method of selenium-rich biological floccules | |
| CN110484456B (en) | Trichosporon and application thereof in degradation of ammonia nitrogen in water body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |