CN104250625A - Aerobic denitrifying bacterium strain and application thereof to water denitrification - Google Patents
Aerobic denitrifying bacterium strain and application thereof to water denitrification Download PDFInfo
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- CN104250625A CN104250625A CN201410229739.XA CN201410229739A CN104250625A CN 104250625 A CN104250625 A CN 104250625A CN 201410229739 A CN201410229739 A CN 201410229739A CN 104250625 A CN104250625 A CN 104250625A
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- aerobic denitrifying
- denitrifying bacteria
- water body
- nitrogen
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Abstract
An aerobic denitrifying bacterium strain belongs to Pseudomonas species, is named as AO0049 and preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO.9089 The aerobic denitrifying bacterium of the invention can be used for denitrification treatment of landscape water and other eutrophication water.
Description
Technical field
The invention belongs to technical field of environmental microorganism, relate to a strain aerobic denitrifying bacteria particularly.
The invention still further relates to screening and the enlarged culturing method of above-mentioned aerobic denitrifying bacteria.
The invention still further relates to the application of above-mentioned aerobic denitrifying bacteria denitrogenation in water body.
Background technology
Along with expanding economy, the living environment of people is also in continuous improvement.City appearance water body, as an important content of urban construction, introduces the life of people.Landscape water body comprises natural lake, artificial lake, man-made tourist site lake and various Landscape River, can regulation domain miniclimate, beautifies the environment.But because source quality is poor, rainwater runoff pollution, bed mud and artifact pollution, landscape water body majority is Static Water simultaneously, and water surface area is little, and water body self-purification ability is poor, very easily cause nitrogen phosphoric and organic accumulation, cause body eutrophication, seriously can cause wawter bloom.
Microorganisms in water reparation is exactly utilize that microorganism absorbs the pollutent in water body by suitable method, enrichment, degrade and remove, reduce or the final process eliminating water pollution, the method has the feature of effecting a permanent cure property, economy and high efficiency in the reparation of static landscape water body.Because landscape water body carbon-nitrogen ratio is lower, and dissolved oxygen concentration is generally more than 4mg/L, therefore, and the strengthening aerobic conversion of nitrogen cycle microflora and remove nitrogen nutrition salt pollutent and the eutrophication of landscape water body to be controlled and ecological reconstruction has great importance.Nitrogenous compound can be converted into the gaseous nitrogen compounds such as nitrogen by aerobic denitrifying bacteria under aerobic conditions, and most ability also with heterotrophic nitrification simultaneously, for water body denitrification provides a kind of brand-new thinking.
Since researchist was separated first and obtained aerobic denitrifying bacteria Thiosphaera pantotropha (Paracoccus pantotrophus) eighties in 20th century, scholars are in succession separated and obtain this type of bacterial strain from the multiple environment such as soil, active sludge, aquaculture water.At present, aerobic denitrification bacterial classification is applied and is rested on the experiment lab scale stage more in landscape water body denitrogenation, and the Main Bottleneck of a mass-producing application is the acquisition of high-performance aerobic denitrification bacterial classification and the foundation of simple and easy, reliable extension cultural method.
Summary of the invention
The object of this invention is to provide a plant height effect aerobic denitrifying bacteria.
Another object of the present invention is to provide a kind of method of screening and the above-mentioned aerobic denitrifying bacteria of enlarged culturing.
Another object of the present invention is that above-mentioned aerobic denitrifying bacteria is applied to landscape water body and other eutrophication water denitrogenations.
For achieving the above object, aerobic denitrifying bacteria provided by the invention, for Rhodopseudomonas (Pseudomonas) species, Strain Designation is AO0049, this bacterial strain has been preserved in China General Microbiological culture presevation administrative center (CGMCC), and deposit number is CGMCC NO.9089.
Described aerobic denitrifying bacteria is Gram-negative bacteria, and LB solid medium is khaki color, utilizes nitrate to grow for only nitrogen source.
The screening method of aerobic denitrifying bacteria of the present invention, is carried out by the water body of collection cultivating, screening in acclimating substratum, obtains aerobic denitrifying bacteria.
In described screening method, gathering water body is get the surface water of the degree of depth at about 0.5m with sterilizing sampling bottle.
In described screening method, acclimating substratum consists of: KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, pH7.0.
The extension cultural method of aerobic denitrifying bacteria of the present invention, be the aerobic denitrifying bacteria above-mentioned screening obtained, in fermention medium, maintain liquid pH is 6.5-7.5, and dissolved oxygen concentration is at more than 2mg/L, nutrient solution total nitrogen and COD are all down to low concentration, and cultivation of now magnifying completes.
In described extension cultural method, fermention medium consists of: NH
4cl0.4 ~ 1.2g/L; KH
2pO
40.75 ~ 1.5g/L, FeC1
26H
2o0.4 ~ 1.2g/L, CaCl
27H
2o0.1 ~ 0.3g/L, MgSO
47H
2o0.75 ~ 1.2g/L, trisodium citrate 5 ~ 10g/L, pH6.8 ~ 7.5.
Aerobic denitrifying bacteria of the present invention can be applied in landscape water body and other eutrophic water bodies and carry out denitrogenation.
Aerobic denitrifying bacteria of the present invention can reach more than 90% to nitric nitrogen transformation efficiency, and nitrite nitrogen is seldom accumulated, non-secondary pollution.
Aerobic denitrifying bacteria of the present invention is good to the equal removal effect of ammonia nitrogen, nitric nitrogen, nitrite nitrogen and COD in landscape water body, has very large using value.
Accompanying drawing explanation
Fig. 1 is strains A O0049 landscape water denitrification effect in embodiment 2.
Fig. 2 is the phylogenetic evolution tree based on the strains A O0049 of 16S rRNA gene order structure in embodiment 3.
Embodiment
Aerobic denitrifying bacteria provided by the invention is Rhodopseudomonas (Pseudomonas) species, called after AO0049.This bacterial strain is preserved in China General Microbiological culture presevation administrative center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, and preserving number is CGMCC NO.9089, and preservation date is on April 25th, 2014.
Strains A O0049 of the present invention is Gram-negative bacteria, LB solid medium is khaki color, and nitrate can be utilized to grow for only nitrogen source.
The present invention has carried out phylogenetic analysis to strains A O0049, and obtaining the 16S rRNA gene order of total length 1401bp, is KJ734677 in the accession number of GenBank.Through BLAST, (structure of (Basic Local Alignment Search Tool) sequence homology comparison and phylogenetic evolution tree, this bacterial strain of preliminary evaluation is Pseudomonas species.
Strains A O0049 obtains from landscape water body screening, and wherein water sample takes from park, Beijing.Fresh water sample is seeded in aerobic denitrifying bacteria acclimating substratum TB, when total nitrogen concentration is down to lower level in nutrient solution, dibromothymolsulfonphthalein (BTB) isolation medium is adopted to carry out plate screening and adopt TB substratum to carry out the mensuration of bacterial strain aerobic denitrification capability.Through primary dcreening operation with sieve experiment again, obtain a plant height effect aerobic denitrification denitrogenation bacterial strain, called after AO0049.
TB acclimating substratum: KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, pH7.0.
Dibromothymolsulfonphthalein (BTB) isolation medium: Agar20g/L, KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, BTB (1% is dissolved in dehydrated alcohol) 1mL, pH7.0.
The optimization method that strains A O0049 extension of the present invention is cultivated is as follows:
Adopt substratum SCT, maintain liquid pH is 6.5-7.5, and dissolved oxygen concentration is at more than 2mg/L, and cultivate more than 4d, total nitrogen and COD are all down to lower level, and cultivation of now magnifying completes.Substratum SCT:NH
4cl0.4 ~ 1.2g/L; KH
2pO
40.75 ~ 1.5g/L, FeC1
26H
2o0.4 ~ 1.2g/L, CaCl
27H
2o0.1 ~ 0.3g/L, MgSO
47H
2o0.75 ~ 1.2g/L, trisodium citrate 5 ~ 10g/L, pH6.8 ~ 7.5.
Following specific embodiment illustrates technical scheme of the present invention, its objective is to understand the present invention better, instead of for limiting the present invention.
Embodiment 1: in landscape water body, the screening of aerobic denitrifying bacteria and aerobic denitrification capability measure
Sampling position is Beijing's park landscape water body, gets the surface water of the degree of depth at about 0.5m with sterilizing sampling bottle.In Bechtop, 10mL water sample is joined in the TB substratum of 90mL sterilizing, be placed in 30 DEG C, the shaking table of 160rpm rotating speed.Get nutrient solution every 24h, in centrifugal rear mensuration supernatant liquor total nitrogen concentration and calculate nitrogen-removing rate.After about 72h, in nutrient solution, total nitrogen content reduces by more than 80%, completes the acclimating process of aerobic denitrifying bacteria.
Sample from nutrient solution and be diluted to 10 successively
-4, 10
-5, 10
-6, 10
-7, 10
-8, 10
-9six gradients, draw 100 μ L and are evenly placed on 30 DEG C of constant incubators cultivate 2 days to the coating of dibromothymolsulfonphthalein (BTB) isolation medium.Aerobic denitrifying bacteria can utilize nitrate to produce basicity, and periphery of bacterial colonies substratum thus can be made to become blueness.Observe also picking and occur that single bacterium colony of blue halos is to new BTB substratum, trilinear method line purifying, is numbered bacterial strain after purifying and preservation, so far completes the primary dcreening operation of aerobic denitrifying bacteria, totally 20 strains.
Get a ring well-grown thalline from solid medium and be seeded to LB liquid nutrient medium, get the nutrient solution that 10mL is in logarithmic phase, the TB substratum of 100mL sterilizing is seeded to after 8000rpm rotating speed centrifuge washing, 3 repetitions are set, be placed in 30 DEG C, 160rpm rotating speed shaking table is cultivated, and measures nitric nitrogen and nitrite nitrogen concentration in nutrient solution every 24h.Through the cultivation of 48h, 20 strain bacteriums have 18 strain nitrogen removal rates more than 50%, and 3 strains are more than 90%, and wherein strains A O0049 nitrogen removal rate is 98.94%, and nitrite nitrogen semi-invariant is only 0.01mg/L, are the best bacterial strains of efficiencies of nitrogen removal.Table 1 is strains A O0049 denitrification effect in TB substratum.
Dibromothymolsulfonphthalein (BTB) isolation medium: Agar20g/L, KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, BTB (1% is dissolved in dehydrated alcohol) 1mL, pH7.0;
TB acclimating substratum: KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, pH7.0;
LB substratum: Tryptone10g/L, Yeast Extract5g/L, NaCl10g/L, pH7.0.
Embodiment 2: the denitrogenation of strains A O0049 landscape water is tested
With the landscape water of sampling spot for experimental water, measuring its pH is 7.0, ammonia nitrogen, nitric nitrogen, nitrite nitrogen, total nitrogen concentration and COD be respectively 34,44,2,80,30mg/L.Get 500mL water sample in 1L shaking flask, add Trisodium Citrate and regulate carbon-nitrogen ratio to 15:1.
Get in LB substratum and be in logarithmic phase bacterium liquid, centrifugal rear supernatant discarded, add brine twice, by bacterial suspension inoculation in above-mentioned water sample, 30 DEG C, in the shaking table of 160rpm rotating speed concussion cultivate, measure the pH of water sample every 24h, ammonia nitrogen, nitric nitrogen, nitrite nitrogen, total nitrogen concentration and COD.To 48h, 5mg/L is down to by total nitrogen, and COD is down to 20mg/L, and nitrogen removal rate reaches 93.75%.Ammonia nitrogen, nitric nitrogen, the change in concentration of nitrite nitrogen as shown in Figure 2.This landscape water is after strains A O0049 denitrogenation processing, and total nitrogen and COD obtain better removal effect, this bacterial strain has very large application potential in landscape water body and other eutrophic water body denitrogenations as seen.
Embodiment 3: strains A O0019 enlarged culturing method
Based on achievement in research above, can find that strains A O0049 is respond well in landscape water denitrogenation is administered, and in the application of reality, not only will ensure the nitrogen removal performance of bacterial strain, adopt optimization culture to make cell density reach the highest again, be beneficial to formulation investigation and application.Be the optimization method of aerobic denitrifying bacteria enlarged culturing below.
SCT substratum after inclined-plane picking thalline to sterilizing, is placed in 30 DEG C of shaking tables and cultivates with 160rpm rotating speed.About 24h thalli growth, to logarithmic phase, is seeded in the sterile culture flask being placed with 10L fresh culture SCT by after its centrifuge washing in 10% ratio (v/v), cultivates under room temperature.In order to ensure the oxygen demand of aerobic denitrifying bacteria, adopt aerating apparatus with the dissolved oxygen concentration in maintain base at more than 2mg/L.
In bacterial strain enlarged culturing process, maintain liquid pH is 6.5-7.5.Along with the oxidation of ferrous iron, nutrient solution color gradually becomes reddish-brown by light green.Through the cultivation of 5d, about 10mg/L is down to by nutrient solution total nitrogen, and COD is down to about 100mg/L.Now the enlarged culturing of bacterial strain completes, and nutrient solution can be added in reactor or treatment sewage.
SCT fermention medium: NH
4cl0.47g/L; KH
2pO
41.0g/L, FeC1
26H
2o1.07g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1.0g/L, trisodium citrate 5g/L, pH7.0.Table 1: strains A O0049 is denitrification effect in TB substratum
Note: NO
3-N and NO
2-N concentration is three mean values repeating experimental result.
Claims (8)
1. a strain aerobic denitrifying bacteria, for Rhodopseudomonas (Pseudomonas) species, Strain Designation is AO0049, and this bacterial strain has been preserved in China General Microbiological culture presevation administrative center (CGMCC), and deposit number is CGMCC NO.9089.
2. aerobic denitrifying bacteria according to claim 1 is Gram-negative bacteria, and LB solid medium is khaki color, utilizes nitrate to grow for only nitrogen source.
3. the screening method of aerobic denitrifying bacteria described in claim 1, carries out the water body of collection cultivating, screening, obtains aerobic denitrifying bacteria in acclimating substratum.
4. screening method according to claim 3, wherein, gathering water body is get the surface water of the degree of depth at about 0.5m with sterilizing sampling bottle.
5. screening method according to claim 3, wherein, acclimating substratum consists of: KNO
31g/L, KH
2pO
41g/L, FeC1
26H
2o0.5g/L, CaCl
27H
2o0.2g/L, MgSO
47H
2o1g/L, sodium succinate 8.5g/L, pH7.0.
6. the extension cultural method of aerobic denitrifying bacteria described in claim 1, claim 3 is screened the aerobic denitrifying bacteria obtained, in fermention medium, maintain liquid pH is 6.5-7.5, and dissolved oxygen concentration is at more than 2mg/L, nutrient solution total nitrogen and COD all reduce, and cultivation of now magnifying completes.
7. extension cultural method according to claim 6, wherein, fermention medium consists of: NH
4cl0.4 ~ 1.2g/L; KH
2pO
40.75 ~ 1.5g/L, FeC1
26H
2o0.4 ~ 1.2g/L, CaCl
27H
2o0.1 ~ 0.3g/L, MgSO
47H
2o0.75 ~ 1.2g/L, trisodium citrate 5 ~ 10g/L, pH6.8 ~ 7.5.
8. aerobic denitrifying bacteria according to claim 1 carries out the application of denitrogenation in landscape water body and other eutrophic water bodies.
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| CN106754450A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of denitrifying microorganism culture promoter and its preparation method and application |
| CN107162168A (en) * | 2017-05-04 | 2017-09-15 | 浙江省农业科学院 | A kind of biological aerated filter based on microbial augmentation |
| CN107176678A (en) * | 2017-05-04 | 2017-09-19 | 浙江省农业科学院 | A kind of method by functional microorganism intensive treatment livestock breeding wastewater |
| CN107177530A (en) * | 2017-06-03 | 2017-09-19 | 农业部环境保护科研监测所 | A kind of new efficient domestic sewage denitrifier and its application |
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| CN110655199A (en) * | 2018-06-29 | 2020-01-07 | 龙岩学院 | Method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
| CN102115719A (en) * | 2010-10-14 | 2011-07-06 | 江南大学 | Aerobic denitrifying bacterium, and screening method and application thereof |
-
2014
- 2014-05-28 CN CN201410229739.XA patent/CN104250625A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115719A (en) * | 2010-10-14 | 2011-07-06 | 江南大学 | Aerobic denitrifying bacterium, and screening method and application thereof |
| CN102061276A (en) * | 2010-11-15 | 2011-05-18 | 北京大学 | Pseudomonas sp. strain for biological denitrification under low temperature and application thereof |
Cited By (12)
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| CN106754450A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of denitrifying microorganism culture promoter and its preparation method and application |
| CN107162168A (en) * | 2017-05-04 | 2017-09-15 | 浙江省农业科学院 | A kind of biological aerated filter based on microbial augmentation |
| CN107176678A (en) * | 2017-05-04 | 2017-09-19 | 浙江省农业科学院 | A kind of method by functional microorganism intensive treatment livestock breeding wastewater |
| CN107177530A (en) * | 2017-06-03 | 2017-09-19 | 农业部环境保护科研监测所 | A kind of new efficient domestic sewage denitrifier and its application |
| CN108546657A (en) * | 2018-04-09 | 2018-09-18 | 深圳市长隆科技有限公司 | A kind of Bali Ali pseudomonad FX-1 and its application |
| CN108546657B (en) * | 2018-04-09 | 2021-08-17 | 深圳市长隆科技有限公司 | Baria alismoides FX-1 and application thereof |
| CN110655199A (en) * | 2018-06-29 | 2020-01-07 | 龙岩学院 | Method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain |
| CN110655199B (en) * | 2018-06-29 | 2021-12-24 | 龙岩学院 | Method for treating ammonia nitrogen wastewater by using heterotrophic nitrification-aerobic denitrification pseudomonas strain |
| CN110386667A (en) * | 2019-07-18 | 2019-10-29 | 曲阜师范大学 | A kind of denitrification Pseudomonas stutzeri reinforcing anoxic pond sewage denitrification method |
| CN110745966A (en) * | 2019-11-06 | 2020-02-04 | 南京大学 | Binary system of quorum sensing-aerobic denitrifying bacteria for enhancing biomembrane denitrification under low-temperature condition, construction method and application |
| CN110745966B (en) * | 2019-11-06 | 2021-12-17 | 南京大学 | Binary system of quorum sensing-aerobic denitrifying bacteria for enhancing biomembrane denitrification under low-temperature condition, construction method and application |
| CN112870620A (en) * | 2021-01-20 | 2021-06-01 | 南京工业大学 | Application of chaetomium cupreum in denitration of nitrocellulose |
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