CN108546657A - A kind of Bali Ali pseudomonad FX-1 and its application - Google Patents
A kind of Bali Ali pseudomonad FX-1 and its application Download PDFInfo
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Abstract
A kind of Bali Ali pseudomonad FX 1 and its application, the invention belongs to sewage treatment fields, to overcome the shortcomings of at present in sewage water denitrification field, a kind of Bali Ali pseudomonad of present invention offer and its bacteria preparation, the deposit number of Bali Ali pseudomonad is GDMCC No.60335, the nitrogen compound that Bali Ali pseudomonad and its bacteria preparation can effectively be degraded by controlling technological parameter in Treatment of Metal Surface waste water, it is environmentally safe, such as Treatment of Metal Surface waste water, breeding wastewater, pharmacy waste water, petrochemical wastewater, coking wastewater, fertilizer waste water, municipal wastewater, landscape water, food wastewater, soy protein wastewater etc., make total nitrogen qualified discharge, it is preferably to denitrogenate selection.
Description
Technical field
The present invention relates to Bali Ali pseudomonad and its applications, specifically, being about removing Treatment of Metal Surface
Its related application of Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of nitrogenous compound in waste water.
Background technology
With the rapid emergence of China's process industry, the industry such as mechanical processing, plating and smelting is carrying out at metal surface
When reason, it is often necessary to be washed to metal surface, generate a large amount of washings, shower waste water.Treatment of Metal Surface waste water is rich in each
Kind organic matter, high phosphorus, high nitrogen and poisonous and harmful heavy metallic, the nitrogen of high-content can not only cause the eutrophication of water body can also be to people
Body health causes heavy losses, therefore, most important to the processing of such denitrogenation of waste water.
It is chemical method, bioanalysis, life that the more extensive method of Treatment of Metal Surface wastewater application is handled outside Current Domestic
Change combined techniques.Nitrogen in Treatment of Metal Surface waste water exists mostly in the form of nitrate nitrogen, and chemical method can remove in water well
Poisonous and harmful substance and high phosphorus, but can not have good removal effect to nitrate nitrogen.Biological denitrificaion be by will screen or
It is added in Pollution System by the strain that biotechnology obtains, is survived by the energy of nitrogenous compound in sewage, is realized
Degradation to nitrogenous compound.Strain can be both attached on carrier, form high-efficiency biomembrane；It can also be deposited with free state
.
Invention content
To solve the problem above-mentioned, the present invention provides a kind of the following technical solution：
One of the objects of the present invention is to provide a kind of Bali Ali pseudomonads (Pseudomonas balearica)
FX-1, bacterial strain FX-1 are preserved in Guangdong Province Culture Collection (address on March 16th, 2018：Guangzhou is first
5 building, No. 59 building of strong Road 100, Guangdong Microbes Inst, Classification And Nomenclature：Bali Ali pseudomonad (Pseudomonas
Balearica), deposit number：GDMCC No.60335.
The second object of the present invention is to provide the application of above-mentioned Bali Ali pseudomonad FX-1, described Bali Ali
Pseudomonad FX-1 is preferred for useless for Treatment of Metal Surface waste water of degrading, breeding wastewater, pharmacy for nitrogen compound of degrading
Nitrification in water, petrochemical wastewater, coking wastewater, fertilizer waste water, municipal wastewater, landscape water, food wastewater, soy protein wastewater
Object.
Preferably, the nitrogen compound is nitrate nitrogen, nitrite nitrogen and/or ammonia nitrogen.
Preferably, can be carried out under anaerobic environment using Bali Ali pseudomonad FX-1 degradation nitrogen compounds.
Can degrade nitrate nitrogen compound under the conditions of simultaneous oxygen.
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 provided by the invention can be with nitrate nitrogen
It closes object and carries out growth and breeding as only nitrogen source.
A kind of bacteria preparation, the bacteria preparation are prepared using above-mentioned Bali Ali pseudomonad FX-1, the bacterium system
Agent is also used for degradation nitrogen compound, while the bacteria preparation further includes nutritious culture medium.Nutrient medium is selected from beef extract
Any one in peptone culture medium, LB nutrient mediums and minimal medium added with carbon source, wherein carbon source
Preferably sodium acetate.
The ingredient and content of beef-protein medium be：Beef 3g/L, peptone 10g/L, sodium chloride 5g/L, pH=
7.0-8.0 preferably pH=7.4-7.6.
The ingredient and content of LB nutrient mediums be：Yeast powder 5.0g/L, sodium chloride 10.0g/L, peptone 10.0g/L,
PH=7.2~7.6.
The ingredient and content of minimal medium be：Sodium nitrate 10g/L, sodium molybdate 0.08g/L, green vitriol
0.12g/L, bitter salt 0.2g/L, calcium chloride 0.12g/L, potassium dihydrogen phosphate 1g/L, diammonium hydrogen phosphate 1g/L, pH=
7.2-7.6。
The present invention provides Bali Ali pseudomonad (Pseudomonas balearica) FX-1 or bacteria preparations
Application in Treatment of Metal Surface waste water of degrading, applying step are as follows：
A), Bali Ali pseudomonad or bacteria preparation are mixed with pending Treatment of Metal Surface waste water；Bali
Ali's pseudomonad additive amount is the 1.5%-30% that additive amount accounts for wastewater treatment system total volume, the carbon in wastewater treatment system
Nitrogen ratio is 1-3.Preferably, additive amount is the 6%-15% of wastewater volume, and the carbon-nitrogen ratio in wastewater treatment system is 2-2.5.
After Bali Ali pseudomonad or bacteria preparation are mixed with pending Treatment of Metal Surface waste water, viable bacteria in mixed system
Number is 1X105~1X108cell/mL。
B) 1.05~1.81g/L of sodium acetate trihydrate is added as carbon source after, mixing, and is then placed into 20-40 DEG C, preferably
It 30 DEG C, reacts in the shaking bath of 160rpm, reaction samples after a certain period of time；
PH value is 6-8, preferably 6.5-7.5, more preferably 7, be conducive to Bali Ali pseudomonad (Pseudomonas
Balearica) FX-1 growth and breedings in Treatment of Metal Surface water, to accelerate to the nitrate nitrogen in Treatment of Metal Surface water
Close the degradation of object.
Preferably, the nitrate of Treatment of Metal Surface water is 110-130mg/L.
The beneficial effects of the present invention are have good removal effect, to ring to nitrate nitrogen in Treatment of Metal Surface waste water
Border is pollution-free, is preferably to denitrogenate selection.
Description of the drawings
Fig. 1 is the bacterium colony of Bali Ali pseudomonad (Pseudomonas balearica) FX-1 in the embodiment of the present invention 2
Aspect graph；
Fig. 2 is for Bali Ali pseudomonad (Pseudomonas balearica) FX-1 in the embodiment of the present invention 3 and simultaneously
It screens obtained FX-2, FX-5 and situation is removed to the nitrate nitrogen of Treatment of Metal Surface waste water respectively；
Fig. 3 is Bali Ali pseudomonad (Pseudomonas balearica) FX-1 processing gold in the embodiment of the present invention 4
Metal surface handles the nitrate nitrogen of waste water differential responses time, COD removes situation；
Fig. 4 be the embodiment of the present invention 5 provide FX-1 bacterial strains different carbon-nitrogen ratios handle Treatment of Metal Surface water nitrate nitrogen,
Ammonia nitrogen, total nitrogen, COD remove situation；
Fig. 5 be the embodiment of the present invention 6 provide FX-1 bacterial strains Different adding amount handle Treatment of Metal Surface water nitrate nitrogen,
Ammonia nitrogen, total nitrogen, COD remove situation.
Specific implementation mode
To keep technical scheme of the present invention and advantage clearer, below in conjunction with attached drawing and embodiment to the present invention make into
One step it is described in detail.
Experimental method in following embodiments is unless otherwise specified conventional method.Reality as used in the following examples
Material is tested, is conventional biochemical reagent unless otherwise specified, can be commercially available.
Embodiment 1
Screening obtains Bali Ali pseudomonad (Pseudomonas balearica) FX-1, and screening process is as follows：
Step 1), from the ponds China Petrochemical Industry Company sewage treatment plant A/A/O treatment process A, outlet takes activated sludge；
The activated sludge of 5g is seeded in the minimal medium that nitrate nitrogen volume fraction is 0.5% by step 2), in temperature
Degree is 30 DEG C, is cultivated 1 day on the shaking table that rotating speed is 150 revs/min, obtains the first bacterium solution；
Step 3), aspiration step 2) in the first bacterium solution be forwarded to nitrate nitrogen volume fraction be 1.5% inorganic salts culture
In base, is cultivated 1 day on the shaking table that temperature is 30 DEG C, rotating speed is 150 revs/min, obtain the second bacterium solution；
Step 4), aspiration step 3) in the second bacterium solution be forwarded to nitrate nitrogen volume fraction be 2.0% inorganic salts culture
In base, is cultivated 1 day on the shaking table that temperature is 30 DEG C, rotating speed is 150 revs/min, obtain third bacterium solution；
Step 5), aspiration step 4) in third bacterium solution be coated on nitrate nitrogen volume fraction be 2.0% inorganic salts solid
It in culture medium, is cultivated 1 day on the shaking table that temperature is 30 DEG C, rotating speed is 150 revs/min, obtains single bacterium colony；
Single bacterium colony in step 6), picking step 5) is placed in centrifuge tube, with glycerine (volume fraction 30%) 1:1 volume is mixed
It closes, bacterium solution is made, and preserved in -80 DEG C of ultra low temperature freezers.
Embodiment 2
The strain screened to embodiment 1 is identified.
In the embodiment of the present invention 1, four plants of bacterium are screened, FX-1, FX-2, FX-5 are respectively designated as in the present invention.Wherein,
Identified, FX-1 is Bali Ali pseudomonad (Pseudomonas balearica).
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 grows good on Agar Plating
It is good, as shown in Figure 1, bacterium colony is in irregular shape, there is fold, transparent, not of uniform size, 2~4mm of diameter, dry tack free, edge is not
Smooth, Gram's staining is feminine gender, has motility, and oxide growth.Strain growth temperature is 20~40 DEG C, pH 6~9, most suitable
Growth conditions：Growth temperature is 30 DEG C, pH 7.5.
DNA is extracted to the single bacterium colony that the step 5) in embodiment 1 obtains, and DNA sequencing, 16S rRNA are carried out to strain
Gene order is as shown in SEQ ID No.1.Bacterial strain FX-1 has been preserved in Guangdong Province microorganism fungus kind on March 16th, 2018 and has protected
Tibetan center (address：5 building, No. 59 building of Xianlie Middle Road, Guangzhou City 100, Guangdong Microbes Inst, Classification And Nomenclature：Bali Ali
Pseudomonad (Pseudomonas balearica), deposit number：GDMCC No.60335.It is bar that the bacterium is also known as in the present invention
Sharp Ali pseudomonad (Pseudomonas balearica) FX-1.
Embodiment 3
To the nitrification for Bali Ali pseudomonad (Pseudomonas balearica) FX-1 bacterium that embodiment 1 obtains
Object object degradation capability is measured.It is as follows：
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 that step 1), Example 1 obtain and same
When the bacterium solution of FX-2, FX-3 screened, centrifuged 10 minutes in 4000 revs/min, abandon supernatant, practical water is added into precipitation
Again it suspends, obtains Bali Ali pseudomonad (Pseudomonas balearica) FX-1；
Step 2), by Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of 0mL, 8.00mL and
FX-2, the FX-5 screened simultaneously is separately added into the test bottle that number is 1,2,3,4, and nitre state is added into each test bottle
Nitrogen is the Treatment of Metal Surface water of 113.38mg/L and sodium acetate trihydrate (carbon-nitrogen ratio 2.0) to total volume is 80mL, is compiled
It Bali Ali pseudomonad (Pseudomonas balearica) FX-1 in number 1 (control), 2,3,4 and screens simultaneously
The bacterium of FX-2, FX-5 original bacteria liquid is dense in 8.1X107~6.4X108Cell/mL, volume fraction are respectively 0,10%；
Measuring system in step 2) is placed in shaking table by step 3), and at 30 DEG C, 150 revs/min of culture 16h are measured
16h treated nitrate nitrogens, and the nitrate nitrogen removal rate of the Treatment of Metal Surface water after calculation processing.
The results detailed in Table 1 and Fig. 2.
1 different strains of table handle the nitrate and removal rate of Treatment of Metal Surface water
As shown in table 1, it is 10% that 3 plants of denitrifiers, which select additive amount, and when processing time is 16h, FX-1 bacterium are in anoxic ring
In border, nitrate nitrogen removal efficiency when integrating water process 16h to metal surface reaches 98.40%, and nitrate is at this time
1.82mg/L.And FX-2 bacterium are substantially less than FX-1 bacterium with FX-5 bacterium denitrification capabilities, nitrate nitrogen treatment effeciency is respectively 44.23%
With 22.2%.Therefore, the denitrification effect of FX-1 bacterium is more excellent, and FX-1 bacterium is selected to do subsequent research.From figure 2 it can be seen that FX-
Compared with FX-5 bacterium, effect is much better than FX-2 bacterium and FX-5 bacterium for the denitrification effect of 1 bacterium and FX-2 bacterium.
Embodiment 4
It is anti-to the difference of Bali Ali pseudomonad (Pseudomonas balearica) FX-1 bacterium of the acquisition of embodiment 1
Nitrogen compound object degradation capability between seasonable is measured.
It is as follows：
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 that step 1), Example 1 obtain, in
4000 revs/min centrifuge 10 minutes, abandon supernatant, and practical water is added into precipitation and suspends again, obtains Bali Ali pseudomonad
(Pseudomonas balearica)FX-1；
Step 2) distinguishes Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of 0mL, 8.00mL
It is added in the test bottle that number is 0,1,2,3,4,5, and the metal that nitrate nitrogen is 123.65mg/L is added into each test bottle
Surface treatment water and sodium acetate trihydrate (carbon-nitrogen ratio 2.0) to total volume are 80mL, in number 0 (control), 1,2,3,4,5
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 original bacteria liquids bacterium it is dense in 8.1X107~
6.4X108Cell/mL, volume fraction are respectively 0,10%；
Measuring system in step 2) is placed in shaking table by step 3), at 30 DEG C, 150 revs/min of cultures 0h, 8h,
12h, 16h, 20h measure each time point treated nitrate nitrogen, COD and pH, and at the metal surface after calculation processing for 24 hours
Manage the removal rate of the nitrate nitrogen and COD of water.
The results detailed in Table 2 and Fig. 3.
The removal of the nitrate and COD of 2 FX-1 bacterial strain differential responses time-triggered protocol Treatment of Metal Surface water of table
As shown in table 2, respectively to 0h, 8h, 12h, 16h, 20h under the conditions of FX-1 bacterial strain additive amounts are 10%, for 24 hours
PH, nitrate nitrogen and COD variations are investigated, the results showed that with the increase of processing time, nitrate nitrogen removal rate has first to increase to be declined afterwards
Trend.Wherein, when processing time is 16h, FX-1 bacterium nitric efficiency highests, and be 93.20%, nitrate is at this time
8.40mg/L.When being less than 16h between when treated, bacterial strain denitrification process carries out not exclusively, and nitric efficiency is relatively low.When treated
Between when being more than 16h, since carbon source in system or nitrogen source lack, microbial cells activity declines, and causes a part of thalline dead, meeting
Increase the nitrate nitrogen and COD contents in water.Show that reaction time selection 16h is best by the above nitrate nitrogen and COD handling results,
Identical conclusion can also be obtained from Fig. 3.
Embodiment 5
To the different carbon for Bali Ali pseudomonad (Pseudomonas balearica) FX-1 bacterium that embodiment 1 obtains
Nitrogen compound object degradation capability under the conditions of nitrogen ratio is measured.It is as follows：
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 that step 1), Example 1 obtain, in
4000 revs/min centrifuge 10 minutes, abandon supernatant, and practical water is added into precipitation and suspends again, obtains Bali Ali pseudomonad
(Pseudomonas balearica)FX-1；
Step 2) distinguishes Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of 0mL, 8.00mL
It is added in the test bottle that number is 1,2, and the Treatment of Metal Surface that nitrate nitrogen is 119.45mg/L is added into each test bottle
Water and sodium acetate trihydrate (carbon-nitrogen ratio 1.5) to total volume are 80mL, Bali Ali's vacation unit cell in number 1 (control), 2
The bacterium of bacterium (Pseudomonas balearica) FX-1 original bacteria liquids is dense in 8.1X107~6.4X108Cell/mL, volume fraction point
It Wei 0,10%；
Step 3) distinguishes Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of 0mL, 8.00mL
It is added in the test bottle that number is 3,4, and the Treatment of Metal Surface that nitrate nitrogen is 119.45mg/L is added into each test bottle
Water and sodium acetate trihydrate (carbon-nitrogen ratio 2.0) to total volume are 80mL, Bali Ali's vacation unit cell in number 3 (control), 4
The bacterium of bacterium (Pseudomonas balearica) FX-1 original bacteria liquids is dense in 8.1X107~6.4X108Cell/mL, volume fraction point
It Wei 0,10%；
Step 4) distinguishes Bali Ali pseudomonad (Pseudomonas balearica) FX-1 of 0mL, 8.00mL
It is added in the test bottle that number is 5,6, and the Treatment of Metal Surface that nitrate nitrogen is 119.45mg/L is added into each test bottle
Water and sodium acetate trihydrate (carbon-nitrogen ratio 2.5) to total volume are 80mL, Bali Ali's vacation unit cell in number 5 (control), 6
The bacterium of bacterium (Pseudomonas balearica) FX-1 original bacteria liquids is dense in 8.1X107~6.4X108Cell/mL, volume fraction point
It Wei 0,10%；
Step 4), by step 2), step 3), the measuring system in step 4) is placed in shaking table, in 30 DEG C, under, 150 turns/
Minute culture 16h measures 16h treated nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, total nitrogen, COD and pH, and the gold after calculation processing
Metal surface handles the removal rate of the nitrate nitrogen of water, ammonia nitrogen, total nitrogen, COD.
The results detailed in Table 3, table 4, table 5, table 6, table 7 and Fig. 4.
3 FX-1 of table processing Treatment of Metal Surface water difference carbon-nitrogen ratio nitrates and removal rate
As shown in table 3, it is 10% in FX-1 bacterial strain additive amounts, under the conditions of the reaction time is 16h, is to carbon-nitrogen ratio respectively
1.5,2.0 and 2.5 nitrate nitrogen and COD removal effects is investigated, and as a result carbon-nitrogen ratio is higher, and nitrate is lower, nitre state
It is bigger that nitrogen removes rate.Carbon-nitrogen ratio is that 1.5,2.0,2.5 treated that nitrate is respectively：26.94mg/L 4.76mg/
L, 3.33mg/L.FX-1 bacterium utilize NO2 -And NO3 -For the final electron acceptor of respiration, nitrate reductase at nitrogen (N2), it takes off
Nitrogen mechanism is：NO3 -→NO2 -→N2↑.Denitrifying bacteria is mostly heterotrophic bacterium, and heterotrophic denitrification bacterium can be with organic
Object is carbon source, and nitrate nitrogen is that the energy carries out denitrification, and biochemical process is：5CH3COOH+8NO3 —→6H2O+10CO2↑+
4N2↑+8OH—+ energy.The height of carbon source concentration affects the process of denitrification.When carbon-nitrogen ratio is 1.5, carbon source is relatively not
Foot, nitrate is relatively high, and nitrate nitrogen removal efficiency is 77.44%.When carbon-nitrogen ratio is 2.0, carbon source concentration is suitable, at this time
Nitrate nitrogen removal efficiency reaches 96.00%, and when carbon-nitrogen ratio is 2.5, carbon source is more sufficient, and nitrate nitrogen removal efficiency is up to 97.21%.
4 FX-1 of table processing Treatment of Metal Surface water difference carbon-nitrogen ratio nitrite nitrogen concentration and removal rate
As shown in table 4, strain additive amount be 10%, FX-1 to Treatment of Metal Surface water process 16h when, carbon-nitrogen ratio 1.5,
When carbon source relative deficiency, NO3 -→NO2 -Process is normally carried out, and NO2 -→N2It is suppressed, the intermediate product in denitrification process
NO2 -Accumulation so that nitrite nitrogen concentration increase.When carbon-nitrogen ratio is 2.0 and 2.5, when carbon source is relatively sufficient, NO3 -→
NO2 -With NO2 -→N2Process, which synchronizes, to be carried out, and relatively low nitrite nitrogen concentration is respectively 0.03mg/L and 0.04mg/L, and removal efficiency is
95.77% and 94.37%.
5 FX-1 of table processing Treatment of Metal Surface water difference carbon-nitrogen ratio ammonia nitrogen concentrations and removal rate
As shown in table 5, ammonia nitrogen concentration is 9.80mg/L in raw water sample, and concentration is relatively low.Strain additive amount is 10%, FX-1 pairs
Treatment of Metal Surface water process 16h, to the ammonia nitrogen after Treatment of Metal Surface wastewater treatment when carbon-nitrogen ratio is respectively 1.5,2.0,2.5
Concentration is respectively：2.15mg/L, 1.53mg/L, 1.68mg/L.Ammonia nitrogen removal frank is respectively：78.06%, 84.38%,
82.85%.The result shows that under aerobic environment, FX-1 bacterium have heterotrophic nitrification aerobic denitrifying effect.
6 FX-1 of table processing Treatment of Metal Surface water difference carbon-nitrogen ratio total nitrogen concentrations and removal rate
As shown in table 6, total nitrogen concentration is that 130.10mg/L contains a small amount of ammonia nitrogen and Asia based on nitrate nitrogen in raw water sample
Nitrate nitrogen is practically free of organic nitrogen.Strain additive amount be 10%, FX-1 to Treatment of Metal Surface wastewater treatment 16h when, carbon-nitrogen ratio
It is that 1.5,2.0,2.5 treated that total nitrogen concentration is respectively：30.25mg/L, 7.24mg/L, 6.08mg/L.Nitrogen removal rate point
It is not：76.75%, 94.44%, 95.32%.Carbon-nitrogen ratio is higher in system, and total nitrogen removal efficiency is higher, with nitrate nitrogen removal rate
It is identical.From the angle of cost savings, carbon-nitrogen ratio 2:It is optimal when 0.
7 FX-1 of table processing Treatment of Metal Surface water difference carbon-nitrogen ratio COD concentration and removal rate
As shown in table 7, strain additive amount is 10%, FX-1 to Treatment of Metal Surface wastewater treatment 16h, carbon-nitrogen ratio 1.5,
2.0,2.5 treated COD (COD) that concentration is respectively：80.4mg/L, 43.5mg/L, 67.2mg/L.COD removals point
It is not：83.96%, 93.11%, 92.77%.Most of carbon source consumption is complete in the system that carbon-nitrogen ratio is 1.5 and 2.0, micro- life
Object utilization rate is high.It is reduced compared with utilization rate in 2.0 systems in the system that carbon-nitrogen ratio is 2.5, shows that added carbon source amount is slightly higher, it is excellent
It is optimum carbon nitrogen ratio that select carbon-nitrogen ratio, which be 2.0,.
Embodiment 6
The difference of Bali Ali pseudomonad (Pseudomonas balearica) FX-1 bacterium of the acquisition of embodiment 1 is added
The nitrogenous compound degradation capability of dosage is measured.It is as follows：
Bali Ali pseudomonad (Pseudomonas balearica) FX-1 bacterium that step 1), Example 1 obtain
Liquid takes the bacterium solution of 2.4mL, 4.8mL, 8.0mL, 12mL and 16mL respectively, is centrifuged 10 minutes in 4000 revs/min, abandons supernatant, to
Practical water is added in precipitation to suspend again, obtains Bali Ali pseudomonad (Pseudomonas balearica) FX-1；
Step 2), Bali Ali pseudomonad by the bacterium solution of 0mL, 2.4mL, 4.8mL, 8.0mL, 12mL and 16mL
(Pseudomonas balearica) FX- thalline 1 are separately added into the test bottle that number is 1,2,3,4,5,6, and to each survey
(be made into carbon-nitrogen ratio is the Treatment of Metal Surface water and corresponding sodium acetate trihydrate that addition nitrate nitrogen is 118.98mg/L in trial jar
2.0:1) it is 80mL to total volume, Bali Ali pseudomonad (Pseudomonas in number 1 (control), 2
Balearica) bacterium of FX-1 original bacteria liquids is dense in 8.1X107~6.4X108Cell/mL, volume fraction is respectively 0,3%, 6%,
10%, 15% and 20%；
Measuring system in step 2) is placed in shaking table by step 3), and at 30 DEG C, 160 revs/min of culture 16h are measured
16h treated nitrate nitrogen, nitrite nitrogen, ammonia nitrogen, total nitrogen, COD and pH, and the nitre of the Treatment of Metal Surface water after calculation processing
The removal rate of state nitrogen, ammonia nitrogen, total nitrogen, COD.
The results detailed in Table 8, table 9, table 10, table 11, table 12 and Fig. 4.
8 FX-1 of table processing Treatment of Metal Surface water Different adding amount nitrates and removal rate
As shown in table 8, carbon-nitrogen ratio 2.0 add bacterium solution 3%, 6%, 10%, 15% and 20% pair of gold with volume ratio respectively
Metal surface handles wastewater treatment 16h.The result shows that when bacterial strain additive amount is 3%-6%, bacterial strain dosage is bigger, nitrate nitrogen
Removal efficiency is higher；When bacterial strain additive amount is 10%, FX-1 bacterium nitric efficiencies are up to 96.00%, at this time nitrate
For 4.76mg/L；When bacterial strain additive amount is 15%-20%, carbon source or nitrogen source lack in system, and part thalline is dead, part nitre
The release of state nitrogen, nitrate slightly increase.
9 FX-1 of table processing Treatment of Metal Surface water Different adding amount nitrite nitrogen concentration and removal rate
As shown in table 9, there are Partial digestion, removal rate of the processing 16h blank groups to nitrite nitrogen for nitrite nitrogen in raw water
Have 8.45%.When thalline additive amount is 3%-6%, carbon source is relatively sufficient in system, with the increase of bacterial strain additive amount, nitrous
State nitrogen concentration continuously decreases；When additive amount is 10%, the minimum 0.21mg/L of nitrite nitrogen concentration；When additive amount is 15%-
When 20%, the concentration of nitrite nitrogen is gradually increasing, and illustrates that biomass is more, will produce more metabolites, increases the water Central Asia
The concentration of nitrate nitrogen.
10 FX-1 of table processing Treatment of Metal Surface water Different adding amount ammonia nitrogen concentrations and removal rate
As shown in table 10, carbon-nitrogen ratio 2.0, added respectively with volume ratio bacterium solution 3%, 6%, 10%, 15% and 20% pair
Treatment of Metal Surface wastewater treatment 16h.When additive amount is 3%-6%, additive amount is bigger, and ammonia nitrogen concentration is lower；Work as additive amount
When being 10%, ammonia nitrogen concentration minimizes as 1.84mg/L, illustrates under aerobic condition, FX-1 thalline have heterotrophic nitrification aerobic anti-
Nitrification；When additive amount is 15%-20%, ammonia nitrogen concentration gradually rises, and thalline dosage is more, will produce more generations
It thanks to product, increases the concentration of ammonia nitrogen in water.
11 FX-1 of table processing Treatment of Metal Surface water Different adding amount total nitrogen concentrations and removal rate
As shown in table 11, it is useless to add thalline 3%, 6%, 10% pair of Treatment of Metal Surface respectively by volume for carbon-nitrogen ratio 2
Water process 16h.When additive amount is 3%-6%, additive amount is bigger, and total nitrogen concentration is lower；When additive amount is 10%, total nitrogen is de-
Except efficiency highest, total nitrogen concentration minimizes as 7.03mg/L at this time；When additive amount is 15%-20%, total nitrogen removal efficiency
It slightly reduces, total nitrogen concentration slightly increases in system, but still reaches discharge standard requirement.
12 FX-1 of table processing Treatment of Metal Surface water Different adding amount COD concentration and removal rate
As shown in table 12, bacterium solution 3%, 6%, 10%, 15%, 20% pair of gold are added with carbon-nitrogen ratio respectively by volume for 2
Metal surface handles wastewater treatment 16h.When dosage is 3%-6%, additive amount is bigger, and carbon source consumption is bigger, and COD concentration is got over
It is low.Strain dosage is too low at this time, can neither completely consume carbon source also and can not effectively remove the nitrogen in system；Work as dosage
When 10%, the total nitrogen concentration after strain handles COD in system using carbon source efficiently uses completely, in system in water can reduce
It is extremely below the mark.When dosage is 15%-20%, the carbon source added is excessive, causes COD in water excessively high.Therefore with dosage
10% is advisable.
It is merely a preferred embodiment of the present invention, is not intended to restrict the invention, for the technology of this field described in upper
For personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
SEQUENCE LISTING
<110>The grand Science and Technology Ltd. of Shenzhen mayor
<120>A kind of Bali Ali pseudomonad FX-1 and its application
<130> PJ1810167.06
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 808
<212> DNA
<213> Pseudomonas balearica
<400> 1
tttattagct ggcgcccctt aaacattgca aagtcgagcg gcagcgggtc cttcgggatg 60
ccggcgagcg gcggacgggt gagtaatgcc taggaatctg cctggtagtg ggggataact 120
cggggaaact cgagctaata ccgcatacgt cctacgggag aaagcggggg atcttcggac 180
ctcgcgctac cagatgagcc taggtcggat tagctagttg gtggggtaaa ggctcaccaa 240
ggcgacgatc cgtagctggt ctgagaggat gatcagccac actggaactg agacacggtc 300
cagactccta cgggaggcag cagtggggaa tattggacaa tgggcgaaag cctgatccag 360
ccatgccgcg tgtgtgaaga aggtcttcgg attgtaaagc actttaagtt gggaggaagg 420
gcagtaagct aataccttgc tgttttgacg ttaccgacag aataagcacc ggctaacttc 480
gtgccagcag ccgcggtaat acgaagggtg caagcgttaa tcggaattac tgggcgtaaa 540
gcgcgcgtag gtggtttgat aagttggatg tgaaagcccc gggctcaacc tgggaattgc 600
atccaaaact gtctgactag agtatggcag agggtggtgg aatttcctgt gtagcggtga 660
aatgcgtaga tataggaagg aacaccagtg gcgaaggcga ccacctgggg ctaatactga 720
cactgaggtg cgaaagcgtg gggagcaaac aggattagat accctggtat tccacgccgt 780
aaacgatgtc gactagccgt tgggatcc 808
Claims (9)
1. a kind of Bali Ali pseudomonad FX-1, which is characterized in that its deposit number is GDMCC No.60335.
2. a kind of application of Bali Ali pseudomonad FX-1, which is characterized in that Bali Ali pseudomonad FX-1 is used
In degradation nitrogen compound.
3. the application of Bali Ali pseudomonad FX-1 as claimed in claim 2, which is characterized in that described Bali Ali is false
Monad FX-1 is useless for Treatment of Metal Surface waste water of degrading, breeding wastewater, pharmacy waste water, petrochemical wastewater, coking wastewater, chemical fertilizer
Nitrogen compound in water, municipal wastewater, landscape water, food wastewater, soy protein wastewater.
4. the application of Bali Ali pseudomonad FX-1 as described in Claims 2 or 3 is any, which is characterized in that the nitrogen
Compound is nitrate nitrogen, nitrite nitrogen and/or ammonia nitrogen.
5. the application of Bali Ali pseudomonad FX-1 as described in Claims 2 or 3 is any, which is characterized in that apply Bali
Ali pseudomonad FX-1 degradation nitrogen compounds are carried out under anaerobic environment.
6. the application of Bali Ali pseudomonad FX-1 as described in Claims 2 or 3 is any, which is characterized in that with Bali Ah
In pseudomonad FX-1 degradation nitrogen compound when, additive amount accounts for the 1.5%-30% of wastewater treatment system total volume, wastewater treatment
Carbon-nitrogen ratio in system is 1-3.
7. the application of Bali Ali pseudomonad FX-1 as claimed in claim 6, which is characterized in that use Bali Ali's vacation unit cell
When bacterium FX-1 degradation nitrogen compounds, additive amount is the 6%-15% of wastewater volume, and the carbon-nitrogen ratio in wastewater treatment system is 2-
2.5。
8. the application of Bali Ali pseudomonad FX-1 as described in Claims 2 or 3 is any, which is characterized in that bar
After sharp Ali pseudomonad FX-1 is mixed with pending Treatment of Metal Surface waste water, viable count is 1X10 in mixed system5~
1X108cell/mL。
9. a kind of bacteria preparation, which is characterized in that the bacteria preparation uses Bali Ali pseudomonad as described in claim 1
FX-1 is prepared.
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| CN113684159A (en) * | 2021-10-27 | 2021-11-23 | 佛山市玉凰生态环境科技有限公司 | Palyra barbadensis and application thereof in denitrification and dephosphorization of river water body |
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