CN110697883B - Method for strengthening denitrification function of membrane bioreactor and slowing down membrane pollution - Google Patents
Method for strengthening denitrification function of membrane bioreactor and slowing down membrane pollution Download PDFInfo
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- CN110697883B CN110697883B CN201810745834.3A CN201810745834A CN110697883B CN 110697883 B CN110697883 B CN 110697883B CN 201810745834 A CN201810745834 A CN 201810745834A CN 110697883 B CN110697883 B CN 110697883B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention belongs to the technical field of sewage treatment, and particularly discloses a method for strengthening the denitrification function of a membrane bioreactor and slowing down membrane pollution. The method specifically comprises the following steps: adding pseudomonas W12 liquid microbial inoculum according to the volume ratio of 0.1-1.0% in a normally operated membrane bioreactor, and adding once every 5-10 days; adding a bacillus subtilis SZX11 liquid microbial inoculum according to the volume ratio of 0.2-1.5%, and adding the bacillus subtilis SZX11 liquid microbial inoculum once every 7-15 days. According to the invention, the pseudomonas liquid microbial inoculum and the bacillus subtilis liquid microbial inoculum are added into the activated sludge of the membrane bioreactor, so that ammonia nitrogen can be converted into nitrate nitrogen under aerobic condition, and the membrane bioreactor has a denitrification function, and the total nitrogen removal capability of the membrane bioreactor is enhanced; and the mass induction signal molecules on the surface of the hollow fiber microfiltration membrane can be quickly decomposed, the formation of the microbial membrane on the surface of the microfiltration membrane is slowed down, the pollution of the microfiltration membrane is reduced, the service life of the microfiltration membrane is prolonged, and the operation and maintenance cost is reduced.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to a microorganism regulation and control method for strengthening the denitrification function of a membrane bioreactor and slowing down membrane pollution.
Background
A Membrane Bioreactor (MBR) is a high-efficiency sewage treatment technology combining a membrane separation unit and a biological treatment unit, a membrane component is used for replacing a secondary sedimentation tank, the concentration of high-activity sludge is kept in the bioreactor, the organic load of biological treatment is improved, the occupied area of sewage treatment facilities is reduced, and the sludge amount is reduced by keeping low sludge load. Compared with the traditional biochemical water treatment technology, the membrane bioreactor has the following advantages: the treatment efficiency is high, and the effluent quality is good; the equipment is compact and the occupied area is small; automatic control is easy to realize, and operation management is convenient. However, membrane flux attenuation caused by membrane fouling is rapid, the cost of the required membrane material is high, and the cleaning energy consumption of membrane fouling is high, and these adverse factors hinder the popularization and application of the membrane bioreactor technology.
One typical behavior of quorum sensing is biofilm formation. When the individual microorganism reaches a certain density, the gene expression of quorum sensing signal molecules will produce mucopolysaccharides, resulting in the formation of structural biofilms. Earlier bacterial quorum sensing studies focused on the study of pathogenic bacteria, which when bacteria reached sufficiently high population densities could alter gene expression required for infection to induce massive cell cooperation for host infection, and which could evade the host immune response, thereby successfully establishing infection (Dangl, et al, Nature.2001.411: 826-. In recent years, extensive studies have also been carried out in the field of environmental microbiology, such as: in drip irrigation systems, clogging is caused by the formation of microbial films in the emitter flow channels (Yan, et al, Journal of Environmental Sciences,2009,21(6):834-841.) with great ease. A bacillus subtilis SZX11 strain capable of efficiently degrading quorum sensing signal molecules is separated and used for preventing and controlling the formation of microbial membranes on the surface of an MBR microfiltration membrane, so that the occurrence of blockage is slowed down, and a good effect is obtained.
Moreover, because the membrane bioreactor is operated in a continuous aeration state for treating sewage and lacks a denitrification process, although ammonia nitrogen can be rapidly oxidized into nitrate nitrogen, the removal effect of total nitrogen in the sewage is not ideal.
Heterotrophic nitrification refers to the process of converting ammonia nitrogen into hydroxylamine, nitrite and nitrate while utilizing organic substrates by microorganisms. Aerobic denitrification refers to the fact that microorganisms can perform respiration by using oxygen and nitrate or nitrite as electron acceptors at the same time. The discovery of heterotrophic nitrifying bacteria, aerobic denitrifying bacteria and the like breaks through the recognition that the Nitrification reaction can only be completed by autotrophic bacteria and the Denitrification reaction can only be carried out under anaerobic conditions in the traditional theory, so that the Synchronous Nitrification and Denitrification (SND) becomes possible.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a method for strengthening the denitrification function of a membrane bioreactor and slowing down membrane pollution.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
the invention provides a method for strengthening the denitrification function of a membrane bioreactor and slowing down membrane pollution, wherein a pseudomonas liquid microbial inoculum is added into a normally running membrane bioreactor according to the volume ratio of 0.1-1.0 percent and is added once every 5-10 days; adding a bacillus subtilis liquid microbial inoculum according to the volume ratio of 0.2-1.5%, and adding the bacillus subtilis liquid microbial inoculum once every 7-15 days.
Preferably, the pseudomonas liquid microbial inoculum is added according to the volume ratio of 0.3 percent and is added once every 7 days; adding a bacillus subtilis liquid microbial inoculum according to the volume ratio of 0.5 percent, and adding the bacillus subtilis liquid microbial inoculum once every 10 days.
The first adding time of the two bactericides is the same, namely the first adding is carried out when the sewage treatment is started.
The preparation method of the pseudomonas liquid microbial inoculum comprises the following steps:
(1) preparing a pseudomonas liquid culture medium: 3-6 parts of ammonium sulfate, 0.2-0.5 part of disodium hydrogen phosphate and 1000 parts of potato starch with the COD concentration of 20-50 g/L, and adjusting the pH value of the culture medium to 7.0-7.5; sodium hydroxide can be used for adjusting the pH;
(2) culturing liquid pseudomonas seeds: inoculating pseudomonas preserved on an inclined plane into a triangular flask filled with the pseudomonas liquid culture medium, and performing shaking culture at the temperature of 25-35 ℃ and the rotation speed of 120-200 rpm for 16-30 hours to obtain pseudomonas liquid seeds;
(3) preparing a pseudomonas liquid microbial inoculum: inoculating 2-10% by volume of pseudomonas liquid seeds into a fermentation tank filled with the pseudomonas liquid culture medium, and introducing the seeds at 25-35 DEG CCulturing in sterile air for 12-30 hours; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 2 multiplied by 109And (5) obtaining the pseudomonas liquid microbial inoculum by using the CFU/ml of the solution.
The preparation method of the bacillus subtilis liquid microbial inoculum comprises the following steps:
(1) preparing a bacillus subtilis liquid culture medium: 2-5 parts of ammonium sulfate, 0.2-0.3 part of disodium hydrogen phosphate and 1000 parts of potato starch with the COD concentration of 20-50 g/L, and adjusting the pH value of the culture medium to 7.0-7.8; sodium hydroxide can be used for adjusting the pH;
(2) culturing liquid seeds of the bacillus subtilis: inoculating the bacillus subtilis preserved on the inclined plane into a triangular flask filled with the bacillus subtilis liquid culture medium, and performing shaking culture at the temperature of 30-37 ℃ and the rotation speed of 120-200 rpm for 15-25 hours to obtain bacillus subtilis liquid seeds;
(3) preparing a bacillus subtilis liquid microbial inoculum: inoculating 2-10% by volume of Bacillus subtilis liquid seeds into a fermentation tank filled with the Bacillus subtilis liquid culture medium, and introducing sterile air to culture for 12-25 hours at 30-37 ℃; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 3 multiplied by 109And (3) more than CFU/ml, thus obtaining the bacillus subtilis liquid microbial inoculum.
By adding the two microbial agents into the membrane bioreactor, the total nitrogen removal rate of the bioreactor can be improved by more than 30%, and the backwashing period of the microfiltration membrane is effectively prolonged by more than 1/3; thereby improving the effluent quality of the membrane bioreactor and obviously improving the filtration efficiency of the microfiltration membrane.
Furthermore, the strain used for preparing the Pseudomonas liquid microbial inoculum is obtained by separating and screening activated sludge from a sewage treatment plant, and the screened Pseudomonas W12 strain is classified and named as Pseudomonas (Pseudomonas sp), and can simultaneously play the functions of nitrification and denitrification under the normal aeration condition of MBR. The strain is preserved in the China general microbiological culture Collection center in 2016, 5, 17 and the preservation number is as follows: CGMCC No.12460, preservation Unit Address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101.
the pseudomonas strain is used for preparing a microbial inoculum, and the microbial inoculum is properly added into a membrane pool of MBR, so that the removal effect of the system on the total nitrogen in the sewage can be improved under the condition of normal aeration.
Furthermore, the strain used in the preparation of the bacillus subtilis liquid microbial agent is a bacillus subtilis SZX11 strain which is separated from the phyllosphere of pine trees and has the capability of efficiently degrading microbial quorum sensing signal molecules, and is classified and named as bacillus subtilis. The strain is preserved in China general microbiological culture Collection center in 16.09.2013, with the preservation number being: CGMCC No.8188, preservation unit address: west road No.1, north west of the republic of kyo, yang, institute of microbiology, academy of sciences of china, zip code: 100101.
the bacillus subtilis strain is used for preparing a microbial inoculum, and is properly added into a membrane pool of an MBR (membrane bioreactor), so that a microbial membrane can be prevented from being formed on a filter membrane under the condition of normal aeration, the blocking process is slowed down, and the backwashing frequency is reduced.
The weight part can be a weight unit known in the field of sewage treatment such as gram, kilogram, ton and the like, and can also be a multiple thereof, such as 1/10, 1/100, 10, 100 and the like.
The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.
The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.
The invention has the beneficial effects that:
the pseudomonas is added into the activated sludge of the membrane bioreactor, so that the pseudomonas can convert ammonia nitrogen into nitrate nitrogen under the aerobic condition, has the denitrification function and strengthens the total nitrogen removal capability of the membrane bioreactor;
the bacillus subtilis is added into the activated sludge of the membrane bioreactor, so that colony induction signal molecules on the surface of the hollow fiber microfiltration membrane are quickly decomposed, the formation of a microbial membrane on the surface of the microfiltration membrane is slowed down, the pollution of the microfiltration membrane is reduced, the service life of the microfiltration membrane is prolonged, and the operation and maintenance cost is reduced.
Detailed Description
The present invention is further illustrated by the following examples. It is to be understood that the following examples are given for illustrative purposes only and are not intended to limit the scope of the present invention. Various modifications and alterations of this invention will become apparent to those skilled in the art without departing from the spirit and scope of this invention.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 culture of Pseudomonas having heterotrophic nitrification and aerobic denitrification
Preparing a pseudomonas liquid culture medium: 5 kg of ammonium sulfate, 0.3 kg of disodium hydrogen phosphate and 1 ton of potato starch waste with the COD concentration of 30g/L, and adjusting the pH value of the culture medium to 7.0 by using sodium hydroxide.
Culturing liquid pseudomonas seeds: inoculating pseudomonas preserved on the inclined plane into a triangular flask filled with a liquid culture medium, and performing shaking culture at the temperature of 30 ℃ and 150 rpm for 24 hours to obtain pseudomonas liquid seeds;
preparing a pseudomonas liquid microbial inoculum: inoculating 5% volume ratio of Pseudomonas liquid seed into a fermentation tank filled with liquid culture medium, and culturing at 30 deg.C in sterile air for 20 hr; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 2 multiplied by 109And (5) obtaining the pseudomonas liquid microbial inoculum by using the CFU/ml of the solution.
Example 2 Bacillus subtilis culture for efficient degradation of quorum sensing Signal molecules
Preparing a bacillus subtilis liquid culture medium: 3 kg of ammonium sulfate, 0.25 kg of disodium hydrogen phosphate and 1 ton of potato starch waste with the COD concentration of 30g/L, and adjusting the pH value of the culture medium to 7.5 by using sodium hydroxide.
Culturing liquid seeds of the bacillus subtilis: inoculating the bacillus subtilis preserved on the inclined plane into a triangular flask filled with a liquid culture medium, and performing shaking culture at 35 ℃ and 150 rpm for 20 hours to obtain bacillus subtilis liquid seeds;
preparing a bacillus subtilis liquid microbial inoculum: inoculating 5% volume ratio Bacillus subtilis liquid seed into a fermentation tank filled with liquid culture medium, and culturing at 35 deg.C with sterile air for 16 hr; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 3 multiplied by 109And (3) more than CFU/ml, thus obtaining the bacillus subtilis liquid microbial inoculum.
Example 3 Membrane pollution prevention and control by adding microbial inoculum to enhance MBR denitrification
Adding the pseudomonas liquid microbial inoculum prepared in the example 1 according to the volume ratio of 0.3% in a normally operated domestic sewage treatment membrane bioreactor, and adding the pseudomonas liquid microbial inoculum once every 7 days; the bacillus subtilis liquid microbial inoculum is added according to the volume ratio of 0.5 percent and is added once every 10 days. After the two microbial agents are added, the average removal rate of the membrane bioreactor to total nitrogen is improved by 37%, and the backwashing period of the microfiltration membrane is prolonged from the original backwashing for 1 time in 30 minutes to the backwashing for 1 time in 50 minutes.
Example 4 adding microbial inoculum to enhance MBR denitrification and prevention and control membrane pollution
Adding the pseudomonas liquid microbial inoculum prepared in the example 1 according to the volume ratio of 0.1% in a normally operated domestic sewage treatment membrane bioreactor, and adding the pseudomonas liquid microbial inoculum once every 5 days; the bacillus subtilis liquid microbial inoculum is added according to the volume ratio of 0.2 percent and is added once every 7 days. After the two microbial agents are added, the average removal rate of the membrane bioreactor to the total nitrogen is improved by 29 percent, and the backwashing period of the microfiltration membrane is prolonged from the original backwashing for 1 time in 30 minutes to the backwashing for 1 time in 45 minutes.
Example 5 Membrane contamination prevention and control by MBR denitrification enhanced by adding microbial inoculum
Adding the pseudomonas liquid microbial inoculum prepared in the example 1 according to the volume ratio of 1.0% in a normally operated domestic sewage treatment membrane bioreactor, and adding the pseudomonas liquid microbial inoculum once every 10 days; the bacillus subtilis liquid microbial inoculum is added according to the volume ratio of 1.5 percent and is added once every 15 days. After the two microbial agents are added, the average removal rate of the membrane bioreactor to the total nitrogen is improved by 43 percent, and the backwashing period of the microfiltration membrane is prolonged from the original backwashing for 1 time in 30 minutes to the backwashing for 1 time in 90 minutes.
It should be understood that the technical solutions of the above embodiments, in which the amounts of reagents or raw materials used are proportionally increased or decreased, are substantially the same as those of the above embodiments.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (6)
1. A method for strengthening the denitrification function of a membrane bioreactor and slowing down membrane pollution is characterized in that a pseudomonas liquid microbial inoculum is added into a normally running membrane bioreactor according to the volume ratio of 0.1-1.0 percent and is added once every 5-10 days; adding a bacillus subtilis liquid microbial inoculum according to the volume ratio of 0.2-1.5%, and adding the bacillus subtilis liquid microbial inoculum once every 7-15 days; the preservation number of the pseudomonas is as follows: CGMCC number 12460, the preservation number of the Bacillus subtilis is as follows: CGMCC number 8188.
2. The method according to claim 1, wherein the pseudomonas liquid bacterial agent is added in a normally operating membrane bioreactor according to the volume ratio of 0.3 percent and is added once every 7 days; adding a bacillus subtilis liquid microbial inoculum according to the volume ratio of 0.5 percent, and adding the bacillus subtilis liquid microbial inoculum once every 10 days.
3. The method according to claim 1 or 2, wherein the preparation method of the pseudomonas liquid inoculant comprises the following steps:
(1) preparing a pseudomonas liquid culture medium: 3-6 parts of ammonium sulfate, 0.2-0.5 part of disodium hydrogen phosphate and 1000 parts of potato starch with the COD concentration of 20-50 g/L, and adjusting the pH value of the culture medium to 7.0-7.5; adjusting pH with sodium hydroxide;
(2) culturing liquid pseudomonas seeds: inoculating pseudomonas preserved on an inclined plane into a triangular flask filled with the pseudomonas liquid culture medium, and performing shaking culture at the temperature of 25-35 ℃ and the rotation speed of 120-200 rpm for 16-30 hours to obtain pseudomonas liquid seeds;
(3) preparing a pseudomonas liquid microbial inoculum: inoculating 2-10% by volume of pseudomonas liquid seeds into a fermentation tank filled with the pseudomonas liquid culture medium, and introducing sterile air to culture for 12-30 hours at 25-35 ℃; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 2 multiplied by 109And (5) obtaining the pseudomonas liquid microbial inoculum by using the CFU/ml of the solution.
4. The method as claimed in claim 1 or 2, wherein the preparation method of the bacillus subtilis liquid agent comprises the following steps:
(1) preparing a bacillus subtilis liquid culture medium: 2-5 parts of ammonium sulfate, 0.2-0.3 part of disodium hydrogen phosphate and 1000 parts of potato starch with the COD concentration of 20-50 g/L, and adjusting the pH value of the culture medium to 7.0-7.8; adjusting pH with sodium hydroxide;
(2) culturing liquid seeds of the bacillus subtilis: inoculating the bacillus subtilis preserved on the inclined plane into a triangular flask filled with the bacillus subtilis liquid culture medium, and performing shaking culture at the temperature of 30-37 ℃ and the rotation speed of 120-200 rpm for 15-25 hours to obtain bacillus subtilis liquid seeds;
(3) preparing a bacillus subtilis liquid microbial inoculum: inoculating 2-10% by volume of Bacillus subtilis liquid seeds into a fermentation tank filled with the Bacillus subtilis liquid culture medium, and introducing sterile air to culture for 12-25 hours at 30-37 ℃; counting the cultured fermentation liquid by plating bacterial colony, the number of viable bacteria reaches 3 multiplied by 109And (3) more than CFU/ml, thus obtaining the bacillus subtilis liquid microbial inoculum.
5. The method of claim 1, wherein the total nitrogen removal rate of the membrane bioreactor is increased by more than 30%.
6. The method of claim 1 or 5, wherein the backwash cycle of the microfiltration membrane in the membrane bioreactor is prolonged above 1/3.
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