CN116354506B - Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor - Google Patents
Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000010802 sludge Substances 0.000 claims abstract description 81
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 38
- VIHAEDVKXSOUAT-UHFFFAOYSA-N but-2-en-4-olide Chemical compound O=C1OCC=C1 VIHAEDVKXSOUAT-UHFFFAOYSA-N 0.000 claims abstract description 14
- 230000000694 effects Effects 0.000 claims abstract description 12
- 239000010865 sewage Substances 0.000 claims abstract description 7
- 239000012636 effector Substances 0.000 claims abstract description 4
- 239000002351 wastewater Substances 0.000 claims description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- GSDSWSVVBLHKDQ-UHFFFAOYSA-N 9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid Chemical compound FC1=CC(C(C(C(O)=O)=C2)=O)=C3N2C(C)COC3=C1N1CCN(C)CC1 GSDSWSVVBLHKDQ-UHFFFAOYSA-N 0.000 claims description 9
- 229960001699 ofloxacin Drugs 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000005273 aeration Methods 0.000 claims description 6
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
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- 239000000203 mixture Substances 0.000 claims description 4
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- 239000011573 trace mineral Substances 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 3
- 239000012498 ultrapure water Substances 0.000 claims description 3
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 48
- 230000001580 bacterial effect Effects 0.000 abstract description 8
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 12
- 239000003242 anti bacterial agent Substances 0.000 description 10
- 229940088710 antibiotic agent Drugs 0.000 description 10
- 238000011081 inoculation Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000001651 autotrophic effect Effects 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- -1 common waste water Chemical compound 0.000 description 2
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- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000032770 biofilm formation Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000018612 quorum sensing Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
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- 230000001360 synchronised effect Effects 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Activated Sludge Processes (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
A method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification by stress of a high-concentration quorum sensing inhibitor belongs to the field of sewage biological treatment. The method comprises 4 stages: stage 1 is the culture of activated sludge; the 2 nd stage is a stress stage of a high-concentration quorum sensing inhibitor, 2 (5H) -furanone is selected, and 30mg/L to 80mg/L of 2 (5H) -furanone acts on an activated sludge system to realize heterotrophic nitrification-aerobic denitrification high-efficiency denitrification; stage 3 is the post-effector phase of quorum sensing inhibitor stress; and 4, enhancing the denitrification effect of the common activated sludge by utilizing the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification activated sludge under the low-temperature condition. The invention can reach the total nitrogen removal rate of more than 85% in the stress stage, can still maintain the total nitrogen removal rate of more than 70% in the post-effect stage, reduces the generation of bacterial drug resistance, and has certain impact resistance to severe environments such as low temperature.
Description
Technical Field
The invention belongs to the field of biological denitrification of sewage, and particularly relates to a method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification by stress of a high-concentration quorum sensing inhibitor. Is suitable for the industrial wastewater treatment fields such as domestic wastewater, medical wastewater, cultivation wastewater, agricultural wastewater and the like.
Background
Antibiotics play a role in sterilizing or inhibiting bacterial growth by interfering with the metabolism of microorganisms, and effectively treat infectious diseases such as bacterial infection. The alternative strategy of combating bacterial infections with antibiotics, i.e. interfering with bacterial quorum sensing by quorum sensing inhibitors, is a promising approach to attenuate the pathogenicity of bacteria by interfering with biofilm formation, virulence factor production or pathogenic gene expression, and not render the bacteria resistant. Quorum sensing inhibitors are considered to be promising antibiotic substitutes and can be used in combination with antibiotics to enhance antibacterial effects, and both are likely to be simultaneously present in the water environment in the future, which together affect the ecological environment.
Nitrogen is the main contaminant of water eutrophication. The main denitrification process at present is a biological method, and biological denitrification mainly depends on autotrophic nitrification and anoxic denitrification. Autotrophic nitrifying bacteria complete nitrification in an aerobic environment, and denitrifying bacteria act on conditions of an anoxic environment and sufficient carbon sources, and the difference of oxygen demand and nutrition conditions makes it difficult for the denitrifying bacteria and the anoxic environment to simultaneously occur in the same reactor. And the heterotrophic nitrification-aerobic denitrification technology breaks this condition limit. Heterotrophic nitrification-aerobic denitrification technology is performed through heterotrophic nitrification-aerobic denitrification flora, and can simultaneously perform nitrification and denitrification under aerobic conditions to enable NH 4 + Direct conversion of N to N 2 O or N 2 Unification of the nitrification and denitrification processes in time and space is realized, and the capital cost is reduced; the alkalinity generated in the denitrification process is used for partially compensating the alkalinity required by the nitrification, so that the operation cost is reduced; the bacteria proliferation speed is high, and the denitrification efficiency is improved. Previous researches show that heterotrophic nitrification-aerobic denitrification bacteria can still maintain higher nitrification and denitrification performance under extreme conditions such as low temperature. However, the current research on heterotrophic nitrification-aerobic denitrification bacteria is mainly focused on the aspects of strain separation and denitrification influencing factors, and the heterotrophic nitrification-aerobic denitrification process does not reach the application level of large-scale engineering due to limited cognition on the heterotrophic nitrification-aerobic denitrification bacteria in the aspects of denitrification mechanism, biology and the like. However, researches show that the stress effect of the high-concentration antibiotics can optimize the flora structure of the activated sludge system according to the response difference of different microorganisms, the denitrification functional bacteria are screened, and the functional flora can still keep activity during the post-effect period, so that the high-efficiency denitrification of the activated sludge system is realized, but the problem of bacterial drug resistance caused by the antibiotics cannot be avoided. And the quorum sensing inhibitor can be used as a substitute of antibiotics, has an action mechanism similar to that of antibiotics, and is considered to realize the screening of the denitrification functional flora through the stress action of the quorum sensing inhibitor with high concentration. Thus, this patent proposes a method of sensing by high concentration populationsThe method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification by the inhibitor stress not only improves the denitrification efficiency of the activated sludge system and effectively reduces the generation of bacterial drug resistance, but also has a certain impact resistance to severe environments such as low temperature and the like.
Disclosure of Invention
The invention aims to provide a method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification by stress of a high-concentration quorum sensing inhibitor. The method solves the problems of difficult enrichment of the denitrification functional bacteria and low denitrification efficiency, effectively relieves the problem of bacterial drug resistance in the activated sludge system, and improves the feasibility of engineering application.
The invention is realized by the following technical scheme:
a. 2 (5H) -furanone with the concentration of 30mg/L-80mg/L is stressed on an activated sludge system, so that heterotrophic nitrification-aerobic denitrification high-efficiency denitrification is realized;
b. culturing activated sludge: activated sludge is collected in an aeration tank of a municipal sewage treatment plant, the collected activated sludge is placed in a reaction vessel, and the activated sludge is diluted by ultrapure water to ensure that MLSS (suspended solids) reaches 10000mg/L; according to the following steps of 1:1, adding wastewater in a volume ratio for aeration, and measuring the ammonia nitrogen concentration of the inlet water and the outlet water to ensure that the ammonia nitrogen removal rate of the outlet water is 95 percent at the end of the period
The activated sludge system is stable as described above and stably operates for 10 cycles;
c. stress phase of high concentration quorum sensing inhibitor: adding the cultured activated sludge into a conical flask, adding wastewater into the conical flask, and uniformly mixing, wherein the volume ratio of the activated sludge to the wastewater is 1:1; the 12 cycles were run continuously under the following conditions: the temperature is 20-25 ℃, the dissolved oxygen DO is 2-8mg/L, and the NH of the inlet water 4 + The N concentration is 35+/-3 mg/L,
COD concentration is 300+ -10 mg/L, trace element concentration is 1mL/L, and pH is regulated by adding sodium bicarbonate
7.5-8.5, wherein the drainage ratio of each period is 50%, meanwhile, the concentration of antibiotics such as ofloxacin contained in the wastewater is 0.5mg/L, and the concentration of quorum sensing inhibitor 2 (5H) -furanone added in the wastewater is 30mg/L and 80mg/L respectively; at cycle 1 andcycle 12 NH 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration; after the 12-cycle operation is finished, the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification of the activated sludge system is realized through the stress of the quorum sensing inhibitor.
The method further comprises the following steps:
d. post-effector phase of quorum sensing inhibitor stress: in the stage after the step c, completely precipitating the activated sludge after the operation of the high-concentration quorum sensing inhibitor stress stage, discharging 50% of supernatant, adding the waste water which does not contain 2 (5H) -furanone and ofloxacin, namely common waste water, continuously operating for 12 periods, and further continuously maintaining the heterotrophic nitrification-aerobic denitrification of the activated sludge system; 13 th cycle and 24 th cycle NH 4 + -N、NO 3 - -N
NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration; after the 24 th period of operation is finished, the active sludge system still maintains the efficient heterotrophic nitrification-aerobic denitrification effect;
the method further comprises the following steps:
e. strengthening the denitrification of common activated sludge at low temperature: the activated sludge for realizing the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification is utilized to strengthen the denitrification effect of a common activated sludge system under the low-temperature condition. Completely precipitating the activated sludge after 24 periods of operation in the step d, discharging 50% of supernatant, inoculating the supernatant into a common activated sludge system according to the proportion of 0% -100%, and adding 1:1, the waste water without 2 (5H) -furanone and ofloxacin, namely common waste water, is continuously operated, the stage is carried out under the low-temperature condition, the temperature is between 14 ℃ and 15 ℃, and the rest operation parameters are consistent with the post-effect stage of quorum sensing inhibitor stress.
The method has the beneficial effects that: after stably culturing activated sludge from municipal sewage treatment plants, the activated sludge is firstly operated for 12 cycles under the stress of a high-concentration quorum sensing inhibitor to ensure that the activated sludge system has heterotrophic nitrification-aerobic denitrification high-efficiency denitrification effect, and the total nitrogen removal rate reaches 86.14% and 81.32% after the 12 cycles are finished. The stability of heterotrophic nitrification-aerobic denitrification in the activated sludge system is maintained by running for 12 more periods in the post-effect stage of quorum sensing inhibitor stress, and finally the total nitrogen removal rate is 72.6% and 70.4% respectively in the 24 th period. The result shows that the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification plays an important role in the denitrification process of the whole activated sludge system after 24-period culture, and the total nitrogen removal rate reaches more than 70% by enriching the heterotrophic nitrification-aerobic denitrification flora to perform synchronous nitrification and denitrification. The activated sludge for realizing the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification is utilized to strengthen the denitrification effect of a common activated sludge system under the low-temperature condition, and when the inoculation proportion is 80%, the average total nitrogen removal rate of the whole system is 59.03%, which shows that heterotrophic nitrification-aerobic denitrification bacteria can still exert the advantages in the activated sludge system, reduce the influence of low temperature on the system, improve the stability of the activated sludge system during the low temperature period and strengthen the low-temperature denitrification effect. The method breaks through the technical bottleneck that heterotrophic nitrification-aerobic denitrification is difficult to realize in the actual sewage treatment process, has strong repeatability and has wide application prospect.
Drawings
FIG. 1 shows total nitrogen removal in cycles 1-12 for control and experimental groups with quorum sensing inhibitor stress concentrations of 30mg/L and 80mg/L, respectively.
FIG. 2 is NH at cycle 12 of the control group and the experimental group with stress concentrations of quorum sensing inhibitor of 30mg/L and 80mg/L, respectively 4 + -N、NO 3 - -N、NO 2 - -N and TN concentration profiles.
FIG. 3 shows total nitrogen removal in cycles 13-24 for control and experimental groups with quorum sensing inhibitor stress concentrations of 30mg/L and 80mg/L, respectively.
FIG. 4 shows stress concentrations of control and quorum sensing inhibitors, respectivelyNH at 24 th cycle of 30mg/L and 80mg/L of experimental group 4 + -N、NO 3 - -N、NO 2 - -N and TN concentration profiles.
FIG. 5 shows total nitrogen removal from activated sludge systems at cycles 1-8 with inoculation ratios of 0%, 20%, 50%, 80% and 100%.
Detailed Description
The present invention will be further described with reference to examples, but the present invention is not limited to the following examples.
Example 1: a method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of a high-concentration quorum sensing inhibitor comprises the following steps:
the manual water distribution is the concentration and composition of wastewater: glucose 0.562g/L, ammonium chloride 0.268g/L, sodium bicarbonate 0.84g/L, monopotassium phosphate 0.03g/L, sodium chloride 0.6g/L, potassium chloride 0.01g/L, ofloxacin 0.5mg/L and trace elements 1mL/L. The concentration and composition of microelements are as follows: na (Na) 2 EDTA 4.29g/L, feCl 2 ·4H 2 O is 1.99g/L, mnCl 2 ·2H 2 O is 0.08g/L, niCl 2 ·6H 2 O is 0.02g/L, coCl.6H 2 O is 0.02g/L, cuCl 2 ·H 2 O is 0.02g/L, znCl 2 0.02g/L NaMoO 4 ·2H 2 O is 0.02g/L, na 2 WoO 4 ·2H 2 O is 0.03g/L, H 3 BO 3 0.06g/L.
The type, concentration and adding mode of the quorum sensing inhibitor are as follows: the quorum sensing inhibitor added into the synthetic wastewater in the 1 st to 12 th periods is 2 (5H) -furanone with the concentration of 30mg/L and 80mg/L. No quorum sensing inhibitor and antibiotics are added into the synthetic wastewater in the 13 th to 24 th periods. The quorum sensing inhibitor and antibiotics are not added into the synthetic wastewater in the stage of strengthening the denitrification of the common activated sludge at low temperature.
Experimental method
Stage one
Culturing activated sludge: activated sludge is collected in an aeration tank of a municipal sewage treatment plant, the collected activated sludge is placed in a reaction vessel, and the activated sludge is diluted by ultrapure water to ensure that MLSS reaches 10000mg/L. According to the following steps of 1:1, adding artificial synthetic wastewater in a volume ratio for aeration, measuring the ammonia nitrogen concentration of the inlet water and the outlet water, ensuring that the ammonia nitrogen removal rate of the outlet water is more than 95% at the end of a period and stably operating for 10 periods, and indicating that the activated sludge system is stable;
stage two
Stress phase of high concentration quorum sensing inhibitor: 125mL of the activated sludge after the culture is taken into a 500mL conical flask, and 125mL of artificial synthetic wastewater is added into the conical flask and uniformly mixed. The control group was run for 12 consecutive cycles under the following conditions: the temperature is 20-25 ℃, the dissolved oxygen DO is 2-8mg/L, and the NH of the inlet water 4 + The concentration of the-N is 35+/-3 mg/L, the concentration of the COD is 300+/-10 mg/L, the concentration of the trace elements is 1mL/L, the pH is regulated and controlled to 7.5-8.5 by adding sodium bicarbonate, and the water discharge ratio per cycle is 50%. The experimental group is added with artificial synthetic wastewater with ofloxacin concentration of 0.5mg/L and 2 (5H) -furanone concentration of 30mg/L and 80mg/L respectively, and the other operation parameters are kept consistent with the control group. NH at cycle 1 and cycle 12 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration. After 12 periods of operation are finished, heterotrophic nitrification-aerobic denitrification high-efficiency denitrification of the activated sludge system is realized through stress of quorum sensing inhibitors;
stage three
Post-effector phase of quorum sensing inhibitor stress: the experimental group at this stage is added with the synthetic wastewater which does not contain 2 (5H) -furanone and ofloxacin, and the operation is continued for 12 periods, so as to maintain the heterotrophic nitrification-aerobic denitrification of the activated sludge system. And (3) completely precipitating the activated sludge at the end of the high-concentration quorum sensing inhibitor stress stage operation, discharging 50% of supernatant, adding 125mL of artificially synthesized wastewater, and uniformly mixing. The control and experimental groups were incubated for 12 cycles with the control and experimental groups operating parameters consistent with the high concentration quorum sensing inhibitor stress phase. 13 th cycle and 24 th cycle NH 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration. After the 24 th period of operation is finished, the active sludge system still maintains the efficient heterotrophic nitrification-aerobic denitrification effect;
stage four
Strengthening the denitrification of common activated sludge at low temperature: the activated sludge for realizing the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification is utilized to strengthen the denitrification effect of a common activated sludge system under the low-temperature condition. The activated sludge after the 24 period operation is completely precipitated and 50% of supernatant is discharged, and the supernatant is inoculated into a common activated sludge system according to the proportion of 0%, 20%, 50%, 80% and 100%, so that the total volume of the activated sludge of the whole system is ensured to be 50mL. Then 1 is added thereto: 1, after being mixed evenly, the mixture is added into a 250mL conical flask. The stage is carried out at low temperature of 14-15deg.C, and the rest operation parameters are consistent with the post-effect stage of quorum sensing inhibitor stress, and then 8 cycles are operated for culturing. Carry out NH in the 8 th cycle 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles only determine NH of inlet and outlet water 4 + -N、NO 3 - -N and NO 2 - -N concentration.
As can be seen from fig. 1 and 2, the total nitrogen removal rate gradually increased by the stress of the quorum sensing inhibitor at high concentrations of 30mg/L and 80mg/L for 12 cycles, and the total nitrogen removal rate in 12 th cycle reached 86.14% and 81.32%, respectively, which were improved by 67.13% and 62.31%, respectively, compared with the total nitrogen removal rate in the control group. TN change curve and NH 4 + The curves of the-N change are substantially coincident, indicating that NO is substantially present during the process 3 - N is accumulated, and heterotrophic nitrification-aerobic denitrification is realized.
As can be seen from fig. 3 and 4, the activated sludge system can still maintain heterotrophic nitrification-aerobic denitrification during the post-effect phase of high concentration quorum sensing inhibitor stress, the average total nitrogen removal rate of the activated sludge system with quorum sensing inhibitor concentration of 30mg/L and 80mg/L is 71.67% and 70.33%, respectively, and the total nitrogen removal rate in 24 th cycle is 72.60% and 70.37%, respectively.
As can be seen from fig. 5, inoculation of 20% heterotrophic nitrification-aerobic denitrification bacteria into the reactor sludge does not significantly increase the total nitrogen removal rate; the total nitrogen removal rate of the activated sludge system with the inoculation proportion of 50 percent is obviously improved compared with that of a 0 percent system; the total nitrogen removal rate is obviously improved at 80% inoculation proportion, and is improved by 32.75% compared with a 0% system, and the average total nitrogen removal rate reaches 59.03%. The method shows that the total nitrogen removal of the whole system under the low-temperature condition is enhanced after the activated sludge with heterotrophic nitrification-aerobic denitrification effect is inoculated. However, with periodic operation, the total nitrogen removal rate of the activated sludge system at each inoculation ratio tends to decrease, indicating that the operating conditions should be improved or other means should be employed to maintain the denitrification efficiency of the activated sludge system for a long period of time after inoculation is completed.
The method can realize heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through enrichment of functional bacterial groups under the action of high-concentration stress, achieves the total nitrogen removal rate of more than 85% in the stress stage, and can still maintain the total nitrogen removal of more than 70% in the post-effect stage. The activated sludge with heterotrophic nitrification-aerobic denitrification is inoculated into a common activated sludge system at a low temperature according to the proportion of 0%, 20%, 50%, 80% and 100%, and the average total nitrogen removal rate of the activated sludge system inoculated at the proportion of 80% is 59.03%, which shows that the denitrification efficiency of the common activated sludge system at the low temperature is enhanced after inoculation. The invention provides technical support for realizing the efficient heterotrophic nitrification-aerobic denitrification of the activated sludge system, reduces the generation of bacterial drug resistance, and ensures that the activated sludge system has a certain impact resistance to severe environments such as low temperature and the like.
The objects and functions of the present invention and methods for achieving these objects and functions will be elucidated by referring to exemplary embodiments. However, the present invention is not limited to the exemplary embodiments disclosed above; this may be implemented in different forms.
Claims (3)
1. A method for effecting heterotrophic nitrification-aerobic denitrification by stress with a high concentration of quorum sensing inhibitor, comprising the steps of:
a. determining to use 2 (5H) -furanone to stress an activated sludge system to realize heterotrophic nitrification-aerobic denitrification;
b. culturing activated sludge: activated sludge is collected in an aeration tank of a municipal sewage treatment plant, the collected activated sludge is placed in a reaction vessel, and the activated sludge is diluted by ultrapure water to ensure that MLSS (suspended solids) reaches 10000mg/L; according to the following steps of 1:1, adding wastewater in a volume ratio for aeration, and measuring the ammonia nitrogen concentration of the inlet water and the outlet water, so that the ammonia nitrogen removal rate of the outlet water is more than 95% at the end of a period, and the activated sludge system is stable when the activated sludge system stably operates for 10 periods;
c. stress phase of high concentration quorum sensing inhibitor: adding the cultured activated sludge into a conical flask, adding wastewater into the conical flask, and uniformly mixing, wherein the volume ratio of the activated sludge to the wastewater is 1:1; the 12 cycles were run continuously under the following conditions: the temperature is 20-25 ℃, the dissolved oxygen DO is 2-8mg/L, and the NH of the inlet water 4 + The concentration of the N is 35+/-3 mg/L, the concentration of COD is 300+/-10 mg/L, the concentration of trace elements is 1mL/L, the pH is regulated and controlled to 7.5-8.5 by adding sodium bicarbonate, the drainage ratio of each period is 50%, the concentration of ofloxacin in the wastewater is 0.5mg/L, and the concentration of quorum sensing inhibitor 2 (5H) -furanone added in the wastewater is 30mg/L or 80mg/L respectively; NH at cycle 1 and cycle 12 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration.
2. A method for effecting heterotrophic nitrification-aerobic denitrification by high concentration of quorum sensing inhibitor stress as in claim 1, further comprising the steps of:
d. post-effector phase of quorum sensing inhibitor stress: in the stage after the step c, precipitating the activated sludge with the high concentration quorum sensing inhibitor, which is operated in the stress stage, completely and discharging 50 percent of supernatant, addingThe wastewater which does not contain 2 (5H) -furanone and ofloxacin is continuously operated for 12 periods, and further the heterotrophic nitrification-aerobic denitrification of the activated sludge system is continuously maintained; 13 th cycle and 24 th cycle NH 4 + -N、NO 3 - -N and NO 2 - Full cycle determination of N concentration, the remaining cycles determining NH of inlet and outlet water only 4 + -N、NO 3 - -N and NO 2 - -N concentration.
3. A method for effecting heterotrophic nitrification-aerobic denitrification by high concentration of quorum sensing inhibitor stress as in claim 2, further comprising the steps of:
e. strengthening the denitrification of common activated sludge at low temperature: the denitrification effect of a common activated sludge system is enhanced under the low-temperature condition by utilizing activated sludge for realizing the heterotrophic nitrification-aerobic denitrification high-efficiency denitrification, 50% of supernatant is completely discharged after the activated sludge is precipitated after 24 periods of operation in the step d, the mixture is inoculated into the common activated sludge system according to the proportion of 0% -100%, and then 1:1, the wastewater which does not contain 2 (5H) -furanone and ofloxacin is continuously operated, no 2 (5H) -furanone is added into the wastewater for stress, the stage is carried out under the low-temperature condition, the temperature is 14-15 ℃, and the other operation parameters are consistent with the post-effect stage of the stress of the quorum sensing inhibitor.
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