CN106350471A - Method for directionally and fast screening enriched broad-spectrum nitrifying bacteria - Google Patents
Method for directionally and fast screening enriched broad-spectrum nitrifying bacteria Download PDFInfo
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- CN106350471A CN106350471A CN201611066280.1A CN201611066280A CN106350471A CN 106350471 A CN106350471 A CN 106350471A CN 201611066280 A CN201611066280 A CN 201611066280A CN 106350471 A CN106350471 A CN 106350471A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 21
- 238000012216 screening Methods 0.000 title claims abstract description 14
- 230000001546 nitrifying effect Effects 0.000 title abstract description 6
- 239000010802 sludge Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims abstract description 10
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 10
- 241000108664 Nitrobacteria Species 0.000 claims description 29
- 230000000694 effects Effects 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 12
- 239000010865 sewage Substances 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 11
- 230000008859 change Effects 0.000 claims description 10
- 235000013619 trace mineral Nutrition 0.000 claims description 10
- 239000011573 trace mineral Substances 0.000 claims description 10
- 230000008569 process Effects 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 7
- 239000006151 minimal media Substances 0.000 claims description 7
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 230000000844 anti-bacterial effect Effects 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 238000005273 aeration Methods 0.000 claims description 4
- 239000002244 precipitate Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 230000002045 lasting effect Effects 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 2
- 230000000630 rising effect Effects 0.000 claims description 2
- -1 that is Chemical compound 0.000 claims description 2
- 239000012531 culture fluid Substances 0.000 claims 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 8
- 229910021529 ammonia Inorganic materials 0.000 abstract description 4
- 241001453382 Nitrosomonadales Species 0.000 abstract description 3
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 238000006396 nitration reaction Methods 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 230000008901 benefit Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 230000002688 persistence Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000005422 algal bloom Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a method for directionally and fast screening enriched broad-spectrum nitrifying bacteria, and belongs to the field of water treatment. Activated sludge is used as a seed source; the structural characters of original AOB (ammonia oxidizing bacteria) and NOB (nitrite oxidizing bacteria) floras are maintained; a high-DO (dissolved oxygen) maintaining method is used; high efficiency of nitration is maintained; under the condition of high DO, FA (free ammonia) and FNA (free nitrous acid) are controlled to be lower than the inhibition concentration on the nitrifying bacteria by pH according to the concentration of ammonia nitrogen and nitrite; high nitrifying efficiency is maintained; the fast culture of the nitrifying bacteria is realized.
Description
Technical field
The invention belongs to water treatment field, particularly to orientation rapid screening enrichment broad spectrum activity nitrobacteria, this technology purport
Fast enriching culture broad spectrum activity nitrobacteria at short notice, including ammonia oxidizing bacteria (aob) and NOB
(nob), the culture of this flora makes to nitrification biological active filling material and the raising of Sewage Plant Ammonia Nitrification performance has widely
Application prospect.
Background technology
With urban modernization, industrial and agriculture development, the pollution of nitrogenous effluent is also increasingly severe, ammonia pollution content mistake
Height can lead to the generation of body eutrophication, makes algal bloom, thus endangering the biology in water.Even destroy ecological flat
Weighing apparatus.Reduce Water element concentration, the pollution that the nitrogen in minimizing water body is brought is one of modern wastewater processing technology
Important topic.The method of conventional process ammonia nitrogen waste water is biological nitrification and denitrification denitrogenation.In existing sewage disposal process, existing dirt
The nitrification efficiency of water treatment plant's activated Sludge System is only 15mg/ (l h), and the substantial amounts of heterotrophic bacteria of association in flora, anti-
, so that nitrobacteria cannot become dominant microflora, the treatment effeciency of therefore ammonia nitrogen waste water is low for other strains such as nitrobacteria.Existing
Have in the experimentation in order to improve nitrification efficiency, how with high-effect nitrobacteria list strain as core, using single bacterial screening,
Culture, enrichment, are carried out to the technology path that system adds.This technology path is there is the problem that single culture is to environment
Bad adaptability.Single culture High-efficient Nitrobacteria, in the sewage disposal system that complicated multiclass antibacterial exists, is hardly formed life
State advantage.
Therefore, for problem above, the present invention proposes orientation rapid screening enrichment broad spectrum activity nitrobacteria.Due to broad spectrum activity
Nitrobacteria Ecological Stabilization is high, easily forms ecological dominance therefore in sewage disposal system.And strain source is in existing sewage
Treatment plant's returned sludge, it has nitrification function originally.Broad spectrum activity nitrobacteria classification screens gained antibacterial function, energy in single
Enough keep the Species structure feature of original mud.The present invention passes through a large amount of enrichment nitrobacterias, produces and has species characteristic
Antibacterial.Based on above premise, the broad spectrum activity nitrobacteria of enrichment culture can also may be used as the preparation of biological nitration active filler
Directly process nitrogenous effluent to be added in the middle of reaction tank, can also serve as the source of enzyme preparation.It is different from traditional single
High efficiency nitrification bacterium, this technology, with quantity as advantage, realizes the raising of nitrification efficiency in existing sludge sewage system.
Content of the invention
It is an object of the invention to developing a kind of fast enriching cultural method based on broad spectrum activity nitrobacteria, by height
Do provides and realizes a large amount of consumption of heterotrophic bacteria and the holding realizing high rate of nitrification；By free ammonia (fa) and free nitrous acid
(fna) effective setting of the effective control of restraining factors, and high-solubility oxygen do (between 1.0mg/l～2.0mg/l) maintains ammonia
Nitrogen and the high oxidization rate of nitrite, it is achieved thereby that the fast culture of nitrobacteria.
The present invention maintains the architectural feature of original aob and nob flora using activated sludge for introduces a collection；Kept using high do
Method, maintains the high efficiency of nitrification；Under conditions of high do, according to ammonia nitrogen and nitrite concentration utilize ph control fa and
Fna is less than the inhibition concentration to nitrobacteria, maintains the high efficiency of nitrification, thus realizing the fast culture of nitrobacteria.
The Enrichment culture method of above-mentioned broad spectrum activity nitrobacteria comprises the steps of
(1) using Sewage Plant returned sludge as strain source, maintained real between 1.5-2.0mg/l by lasting aeration do
A large amount of consumption of existing heterotrophic bacteria, in the case that no ammonia nitrogen adds, are turned into the change of cod and do of the precipitation supernatant of mixed liquor
For the indication index of heterotrophic bacteria consumption process, in the process, the cod comparision contents of raw sewage are low, with the death of heterotrophic bacteria,
The cod content of the precipitation supernatant of mixed liquor increases, and fluctuates up and down, after heterotrophic bacteria is depleted, mixes liquid precipitate
The cod value of supernatant just keeps relative stability, and maintains between 40mg/l～80mg/l, mixing liquid precipitate supernatant do also with
Continuous rising, finally tend towards stability；This process sludge concentration also declines therewith, also reflects heterotrophic bacteria from another angle
Dead；
(2) mud of step (1) is carried out the minimal media of nitrobacteria, the water inlet of minimal media includes nh4Cl basis
Liquid and minimal media liquid, wherein nh4Cl dosage is with nh in culturing sludge system4 +The change of-n concentration and change, inorganic training
In nutrient solution, inorganic matters include na2co3、k2hpo4、kh2po4、mgso4·7h2o、cacl2·2h2O, trace element；Wherein na2co3、
k2hpo4、kh2po4、mgso4·7h2Concentration in culturing sludge system for the o is 20mg/l, cacl2·2h2O 10mg/l, micro-
Secondary element is addition 0.5ml trace element solution, the wherein composition of trace element solution and concentration in every liter of culturing sludge system
As follows: zn2so4·7h2O is 0.12mg/l, namoo4·2h2O is 0.12mg/l, cocl2·6h2O is 0.15mg/l,
mn2so4·h2O is 0.12mg/l, nicl2·6h2O is 0.10mg/l, cuso4·5h2O is 0.03mg/l, fecl3·6h2O is
1.5mg/l.
Minimal media liquid is added using batch (-type) or continuous way, to maintain the basic nutrition condition of nitrobacteria.
In incubation, reaction temperature controls at 25 DEG C, in culturing sludge system do in the range of 1.0～1.2mg/l, ph
For 7.5～8；Control fa and the inhibitory action to nob for the fna, that is, fa controls between 3mg/l～12mg/l, fna controls
Below 0.01mg/l, but utilize the facilitation to nob for the nitrite, that is, nitrite concentration in more than 150mg/l to nob
There is facilitation；Stepping up influent ammonium concentration on the premise of hydraulic detention time holding is constant makes nitrification efficiency continuous
Improve to 150mg/ (l h), or even more than, during this, with the growth of incubation time, sludge concentration is continuously increased, finally
Realize screening and the high concentration enrichment of nitrobacteria (aob and nob).
Preferably: step (2) steps up influent ammonium concentration on the premise of hydraulic detention time holding is constant and makes nitre
Change efficiency to improve constantly to 150mg/ (l h) 8-12 days short time, or even more than.
Preferably: step (2) influent ammonium concentration initial concentration is 100mg/l.
The present invention has the advantages that:
(1) Initial stage of culture persistence aeration and no the adding so that a large amount of heterotrophic bacteria is by endogenous respiration of ammonia nitrogen are passed through
Mode is dead, thus the purification species of antibacterial.
(2) pass through ph, effective cooperation of ammonia nitrogen dosage, fa is control effectively with fna, to realize rapid screening rich
Collection broad spectrum activity nitrobacteria.
(3) in incubation, heterotrophic bacteria consumes after completing, and by the high concentration accumulation of nitrite, realizes nob is given birth to
Long facilitation.
(4) with sewage treatment plant's nitrifying activated sludge as introduces a collection, carry out screening enrichment, maintain original aob and nob flora
Component characteristic.
Specific embodiment
With reference to case study on implementation, the present invention is more specifically described, but embodiments of the present invention are not limited to
This.
Embodiment 1
, through lab scale, effect is significant, concrete operations are as follows for this training method:
(1) utilize tub sbr bioreactor culture returned sludge, carry out the persistence aeration of 4 days by a definite date, and do not add ammonia
Nitrogen, raw sewage concentration (mlss) is 6976mg/l, and do is 1.5-2.0mg/l, and cod is 16.084mg/l, and after 4 days, mlss is changed into
5324mg/l, do content rises to 6.34mg/l, and cod fluctuates after ramping up and down, finally stablize in 60mg/l about.Table
Sight condition shows, a large amount of heterotrophic bacterias consume completely, have entered into the enrichment broad spectrum activity nitrobacteria stage therewith.
(2) on the basis of step (1), by the way of intermittent water inflow, by controlling into water constituent, in system, temperature is
25 DEG C, do is between 1.0mg/l～1.2mg/l, and ph is 7.5～8, and the growth for nitrobacteria provides optimum condition.Water inlet bag
Include nh4Cl basal liquid and mineral solution, wherein nh4Cl dosage is with nh in culturing sludge system4 +The change of-n concentration and
Change, inorganic matters include na2co3、k2hpo4、kh2po4、mgso4·7h2o、cacl2·2h2O, trace element；Wherein na2co3、
k2hpo4、kh2po4、mgso4·7h2Concentration in culturing sludge system for the o is 20mg/l, cacl2·2h2O 10mg/l, micro-
Secondary element is addition 0.5ml trace element solution, the wherein composition of trace element solution and concentration in every liter of culturing sludge system
As follows: zn2so4·7h2O is 0.12mg/l, namoo4·2h2O is 0.12mg/l, cocl2·6h2O is 0.15mg/l,
mn2so4·h2O is 0.12mg/l, nicl2·6h2O is 0.10mg/l, cuso4·5h2O is 0.03mg/l, fecl3·6h2O is
1.5mg/l.
Determine that influent ammonium concentration is increase gradually to 300mg/l by 100mg/l, control fa concentration to be less than the suppression of nob
Concentration, when nitrite concentration is less than 150mg/l, controls fna to be less than 0.01mg/l, through the culture of 10 days, arranges continuous
In the case of mud, sludge concentration steady-state growth 200mg/l, nitrification efficiency is up to 150mg/ (l h).
Claims (4)
1. orientation rapid screening is enriched with the method for broad spectrum activity nitrobacteria it is characterised in that comprising the following steps:
(1) using Sewage Plant returned sludge as strain source, maintained by lasting aeration do realize between 1.5-2.0mg/l different
A large amount of consumption of bacteria, in the case that no ammonia nitrogen adds, are turned to different with the change of cod and do of the precipitation supernatant of mixed liquor
The indication index of bacteria consumption process, in the process, the cod comparision contents of raw sewage are low, with the death of heterotrophic bacteria, mix
The cod content of the precipitation supernatant of liquid increases, and fluctuates up and down, after heterotrophic bacteria is depleted, mixes liquid precipitate supernatant
The cod value of liquid just keeps relative stability, and maintains between 40mg/l～80mg/l, and the do of mixing liquid precipitate supernatant is therewith not yet
Disconnected rising, finally tends towards stability；This process sludge concentration also declines therewith, also reflects the dead of heterotrophic bacteria from another angle
Die；
(2) mud of step (1) is carried out the minimal media of nitrobacteria, the water inlet of minimal media includes nh4Cl basal liquid and nothing
Machine thing culture fluid, wherein nh4Cl dosage is with nh in culturing sludge system4 +The change of-n concentration and change, inorganic matters include
na2co3、k2hpo4、kh2po4、mgso4·7h2o、cacl2·2h2O, trace element；Wherein na2co3、k2hpo4、kh2po4、
mgso4·7h2Concentration in culturing sludge system for the o is 20mg/l, and trace element is to add in every liter of culturing sludge system
The composition of 0.5ml trace element solution, wherein trace element solution and concentration are as follows: zn2so4·7h2O is 0.12mg/l,
namoo4·2h2O is 0.12mg/l, cocl2·6h2O is 0.15mg/l, mn2so4·h2O is 0.12mg/l, nicl2·6h2O is
0.10mg/l, cuso4·5h2O is 0.03mg/l, fecl3·6h2O is 1.5mg/l；
In incubation, reaction temperature controls at 25 DEG C, and in culturing sludge system, in the range of 1.0～1.2mg/l, ph is 7.5 to do
～8；Control fa and the inhibitory action to nob for the fna, that is, fa controls between 3mg/l～12mg/l, fna controls in 0.01mg/l
Hereinafter, but utilize the facilitation to nob for the nitrite, that is, nitrite concentration more than 150mg/l nob is had promotion make
With；Hydraulic detention time keep constant on the premise of step up influent ammonium concentration so that nitrification efficiency improve constantly to
150mg/ (l h), or even more than, during this, with the growth of incubation time, sludge concentration is continuously increased, and finally realizes nitre
Change screening and the high concentration enrichment of antibacterial.
2. it is enriched with the method for broad spectrum activity nitrobacteria it is characterised in that adopting according to the orientation rapid screening described in claim 1
Batch (-type) or continuous way add minimal media liquid, to maintain the basic nutrition condition of nitrobacteria.
3. it is enriched with the method for broad spectrum activity nitrobacteria it is characterised in that step according to the orientation rapid screening described in claim 1
(2) step up influent ammonium concentration on the premise of hydraulic detention time holding is constant and make nitrification efficiency in short time 8-12
It improves constantly to 150mg/ (l h), or even more than.
4. it is enriched with the method for broad spectrum activity nitrobacteria it is characterised in that step according to the orientation rapid screening described in claim 1
(2) influent ammonium concentration initial concentration is 100mg/l.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108726677A (en) * | 2017-04-25 | 2018-11-02 | 湖南大学 | A kind of new process promoting low-carbon-source wastewater treatment |
| CN112746052A (en) * | 2019-10-30 | 2021-05-04 | 中国石油化工股份有限公司 | Culture method for improving resistance of nitrobacteria |
| CN114735811A (en) * | 2022-03-23 | 2022-07-12 | 北京工业大学 | Culture method of short-cut nitrification activated sludge with high accumulation rate |
| CN115261261A (en) * | 2022-06-23 | 2022-11-01 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN116354506A (en) * | 2023-03-21 | 2023-06-30 | 北京工业大学 | Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor |
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| CN104726376A (en) * | 2015-03-27 | 2015-06-24 | 中蓝连海设计研究院 | Continuous large-scale culture method for high-concentration salt-resistant nitrifying bacteria |
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| CN104726376A (en) * | 2015-03-27 | 2015-06-24 | 中蓝连海设计研究院 | Continuous large-scale culture method for high-concentration salt-resistant nitrifying bacteria |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108726677A (en) * | 2017-04-25 | 2018-11-02 | 湖南大学 | A kind of new process promoting low-carbon-source wastewater treatment |
| CN112746052A (en) * | 2019-10-30 | 2021-05-04 | 中国石油化工股份有限公司 | Culture method for improving resistance of nitrobacteria |
| CN112746052B (en) * | 2019-10-30 | 2022-08-12 | 中国石油化工股份有限公司 | Culture method for improving resistance of nitrobacteria |
| CN114735811A (en) * | 2022-03-23 | 2022-07-12 | 北京工业大学 | Culture method of short-cut nitrification activated sludge with high accumulation rate |
| CN115261261A (en) * | 2022-06-23 | 2022-11-01 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN115261261B (en) * | 2022-06-23 | 2023-12-12 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN116354506A (en) * | 2023-03-21 | 2023-06-30 | 北京工业大学 | Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor |
| CN116354506B (en) * | 2023-03-21 | 2024-01-19 | 北京工业大学 | Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor |
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| CN106350471B (en) | 2020-01-24 |
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