CN103588300A - Quick starting method of SBR (Sequencing Batch Reactor) for synchronously denitrifying and removing phosphor - Google Patents
Quick starting method of SBR (Sequencing Batch Reactor) for synchronously denitrifying and removing phosphor Download PDFInfo
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Abstract
The invention discloses a quick starting method of an SBR (Sequencing Batch Reactor) for synchronously denitrifying and removing phosphor, and belongs to the field of sewage treatment. The quick starting method using a batch-injection chronological sequencing batch reactor comprises the following steps: (1), operating the SBR system under an anaerobic/aerobiotic alternation condition, and domesticating and enriching phosphorus-accumulating bacteria, so that the phosphorus-accumulating bacteria intake phosphor under the aerobiotic condition and release phosphor under the anaerobic condition; removing phosphate in water by removing mud; (2), changing an operating way of the system, and enriching denitrifying phosphate-accumulating organisms; increasing an anoxic zone and shortening the aeration time, so that the denitrifying phosphate-accumulating organisms utilize an intracellular carbon source and use nitrate/nitrite as an electron acceptor to intake phosphor; (3), adding a compound fungicide into the reaction, so that biological enhancement is repeatedly coupled with anaerobic/aerobiotic/anoxic/aerobiotic starting method, DPB (Denitrifying Phosphorus-removal Bacteria) are effectively proliferated, glycogen accumulating organisms are restrained, the starting time of the reactor is shortened, and the water outlet effect is improved. The starting way disclosed by the invention operates about 50 days in total, can be used for saving about 30 days and saving the aeration time by about 30% in comparison with an anaerobic/ aerobiotic/ anoxic starting method.
Description
Technical field
The invention belongs to sewage treatment area, more particularly to a kind of synchronous denitrification dephosphorizing SBR quick start methods。
Background technology
In recent years, although China's treatment rate of domestic sewage is greatly improved, but the slow flow water bodies eutrophication problem as caused by nitrogen and phosphorus is still increasingly serious, this is accomplished by the discharge of strict control sewage treatment plant's nitrogen and phosphorus, improves nitrogen, the discharge standard of phosphorus.Although traditional biochemical wastewater treatment technique can be effectively reduced the BOD in sewage5And SS, but 10%~20% can only be removed to the nitrogen and phosphorus in sewage, therefore using new denitrification dephosphorization technique, with effective operation control technology in sewage treatment process, realize that its stable, efficient, operation of low consumption is even more important.
Denitrification dephosphorization technique mainly has SBR techniques, 5 stages improvement Bardenpho technique, MUCT techniques, A2N techniques, Dephanox techniques etc..SBR techniques are because its technique is simple, flexible operation, capacity of resisting impact load is strong, controllability is good, the preferred technique as current Small Urban sewage disposal the advantages of be less prone to sludge bulking.There are the 5 × 10 of 30% ~ 40% in China4m3The Sewage Plant of/below d scales has used SBR techniques.
Denitrifying Phosphate Accumulating Organisms（DPB）Discovery, provide new technical method for the development of denitrification dephosphorization technique, can successfully solve nitrifier, denitrifying bacterium, the contradiction of aerobic phosphorous accumulating bacterium carbon source contention, make full use of intracellular carbon source effectively to inhale phosphorus, realize " carbon is dual-purpose "；Denitrification dephosphorization system includes aerobic/anaerobic, two kinds of methods of operation of aerobic/anaerobic/anoxic, and wherein aerobic/anaerobic/Hypoxic types operation energy consumption is low.
The key of aerobic/anaerobic/oxygen depletion system operation is the suitable condition for ensureing denitrification phosphorus-collecting bacteria growing, and studying aerobic, anoxic, the setting process number of anaerobism, order and each phases-time has important practical significance.Using it is biological reinforced can acceleration system start, shorten the startup time, save aeration energy consumption 30% or so, strengthening system stability.
The content of the invention
Regarding to the issue above, the present invention provides a kind of synchronous denitrification dephosphorizing SBR quick start methods, the sequencing batch reactor being sequentially arranged using the intermittent water inflow in aerobic/anaerobic/anoxic/aerobic alternate run, nitrogen, phosphorus and organic pollution in Small Town Wastewater are removed by the way that Denitrifying Phosphate Accumulating Organisms are biological reinforced.
A kind of synchronous denitrification dephosphorizing SBR quick start methods of the present invention, using sequencing batch reactor SBR, are followed the steps below：
（1）SBR system is run under aerobic/anaerobic alternation condition, domestication enrichment polyP bacteria makes its aerobic phosphorus absorption, anaerobic phosphorus release, the phosphate gone by spoil disposal in water removal；
Specially：SBR system is run under aerobic/anaerobic operating mode, instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 3h precipitate 1h, draining stands 1.5h；DO concentration is less than 0.2mg/L in anaerobic stages water, DO concentration is maintained at 2 ~ 4mg/L in aerobic stage water, DO concentration is less than 0.2mg/L in anaerobic stages water, control 20 ~ 25 DEG C of coolant-temperature gage, it is 7~8 to adjust pH value, runs 20 ~ 30d, and ammonia nitrogen removal frank reaches 90% ~ 99%, TP clearance reaches 70% ~ 80%, and polyP bacteria is enriched with；
（2）Change system operation mode, gathering denitrifying polyP bacteria, increase anoxic section shortens aeration time, and Denitrifying Phosphate Accumulating Organisms inhale phosphorus using intracellular carbon source and by electron acceptor of nitrate nitrogen/nitrite nitrogen；
Specially：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h, DO concentration, water temperature and step in aerobic, anaerobic stages water（1）It is identical, NO in control anaerobic environment3 -Concentration is 30 ~ 40mg/L, and irregular spoil disposal, sludge concentration is 4000 ~ 5000mg/L；7 ~ 10d is run, ammonia nitrogen removal frank reaches that 70% ~ 88%, TP clearance reaches 66% ~ 80%, and Effective multiplication Denitrifying Phosphate Accumulating Organisms suppress the growth of aerobic phosphorous accumulating bacterium and denitrifying bacterium；
（3）Add composite bacteria agent to reactor, make it is biological reinforced with aerobic/anaerobic/anoxic/it is aerobic couple repeatedly, Effective multiplication DPB suppresses fusca xylanase, shortens the reactor start-up time, raising outlet effect,
Specially：From the activated sludge of reactor, Denitrifying Phosphate Accumulating Organisms, i.e. Dell's Ford Pseudomonas and pseudomonas are separated using the obligate culture medium of polyP bacteria, by the Dell Ford Pseudomonas filtered out and pseudomonas by volume（0.25~4）：1 ratio compounding, inoculum concentration is 7 ~ 10% by volume, and inoculation liquid is 35% with reactor volume ratio；System operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h；Aerobic, anaerobic stages DO concentration, water temperature, it is identical with step 1,7 ~ 10d is run, its dephosphorizing rate is up to 90% ~ 96%, and denitrification percent is 85 ~ 91%, water outlet after testing《Urban wastewater treatment firm pollutant emission standard》(GB18918-2002) one-level A standards.
Methods described is suitable for the processing of middle low concentration Small Town Wastewater, i.e. raw water COD mass concentrations 200 ~ 350 mg/L, NH4 +The mg/L of 30 ~ 60 mg/L, TP mass concentration of-N mass concentrations 3 ~ 7.
The obligate culture medium of polyP bacteria separates Denitrifying Phosphate Accumulating Organisms, follows the steps below：Take the activated sludge 10mL in anaerobism section latter stage in sbr reactor device to be put into the triangular flask equipped with 90mL sterilized waters and bead, sludge is fully shaken up using oscillator and smashed, the sludge solutions after smashing dilute 10 successively-1~10-8Times.Pipette the bacterium solution after 0.2mL dilutions respectively with pipette to enter in a diameter of 9cm culture dish, then pour into the obligate 10 ~ 15mL of culture medium of polyP bacteria and be well mixed, after culture dish is inverted in into 30 DEG C of incubated 3d in incubator after culture medium solidifying.The obvious bacterium colony of picking form carries out line separation in culture medium.Filter out Dell Ford Pseudomonas and pseudomonas.
The obligate culture medium chemical composition of polyP bacteria is by mass percentage：CH3COONa·3H2O3680mg/L；Na2HPO4·2H2O28.73mg/L；NH4Cl57.27mg/L；MgSO4·7H2O131.82mg/L；K2SO426.74mg/L；CaCl2·2H2O17.2mg/L；Agar 15000mg/L；HEPES buffer solvents 12000mg/L；Trace element solution 2mL/L.
The trace element solution is configured by the following method：FeSO is added in 1L distilled water4·7H2O5g；CoCl2·6H2O50mg；H3BO350mg；(NH4)6Mo7O24·4H2O1.1g；CuSO4·5H2O1.6g；KI, 10mg；MnCl2·4H2O4g；EDTA50g, stirring shakes up.
The present invention uses Dell Ford Pseudomonas to carry out biological reinforced SBR denitrification dephosphorization systems as Denitrifying Phosphate Accumulating Organisms, improves system denitrogenation dephosphorizing performance.The sequencing batch reactor being sequentially arranged using intermittent water inflow（SBR）, a work period includes：Water inlet, anaerobic phosphorus release, aerobic nitrification, anoxic denitrification inhales phosphorus, aerobic, and precipitation, draining is left unused 7 parts.
SBR system starts domestication 3 stages of process point：Synchronous denitrification dephosphorizing SBR starts, and is run first under aerobic/anaerobic alternation condition, and anaerobic environment control DO concentration is less than 0.2 mg/L, and it is 2 ~ 4mg/L that aerobic environment, which controls DO concentration, is enriched with polyP bacteria；Afterwards, run under aerobic/anaerobic/anoxic/aerobic condition, NO in control anaerobic environment3 -Concentration is 30 ~ 40mg/L, breeds Denitrifying Phosphate Accumulating Organisms；Finally, Dell Ford Pseudomonas and pseudomonas are added into system so that after reactor start-up, water outlet can reach《Urban wastewater treatment firm pollutant emission standard》(GB18918-2002) one-level A standards.
Biological reinforced SBR denitrification dephosphorization techniques it is biological reinforced with aerobic/anaerobic/anoxic/aerobic couple repeatedly can effective denitrogenation dephosphorizing.Stable start of system uses triphasic training method, and by controlling inoculum concentration and inoculation liquid and reactor volume ratio, Effective multiplication DPB suppresses fusca xylanase.This Starting mode runs 50d or so altogether, and the startup method compared to aerobic/anaerobic/anoxic saves 30d or so, saves 30% or so aeration time.TN, TP concentration are respectively reached contained by water outlet《Urban wastewater treatment firm pollutant emission standard》（GB18918-2002）One-level A standards.
Embodiment
Raw water COD mass concentrations 200 ~ 350 mg/L, NH of sewage used in following examples4 +The mg/L of 30 ~ 60 mg/L, TP mass concentration of-N mass concentrations 3 ~ 7.
The obligate culture medium of polyP bacteria separates Denitrifying Phosphate Accumulating Organisms in following examples, follows the steps below：Take the activated sludge 10mL in anaerobism section latter stage in sbr reactor device to be put into the triangular flask equipped with 90mL sterilized waters and bead, sludge is fully shaken up using oscillator and smashed, the sludge solutions after smashing dilute 10 successively-1~10-8Times.Pipette the bacterium solution after 0.2mL dilutions respectively with pipette to enter in a diameter of 9cm culture dish, then pour into the obligate 10 ~ 15mL of culture medium of polyP bacteria and be well mixed, after culture dish is inverted in into 30 DEG C of incubated 3d in incubator after culture medium solidifying.The obvious bacterium colony of picking form carries out line separation in culture medium.The Dell Ford Pseudomonas filtered out and pseudomonas.
The obligate culture medium chemical composition of polyP bacteria is by mass percentage：CH3COONa·3H2O3680mg/L；Na2HPO4·2H2O28.73mg/L；NH4Cl57.27mg/L；MgSO4·7H2O131.82mg/L；K2SO426.74mg/L；CaCl2·2H2O17.2mg/L；Agar 15000mg/L；HEPES buffer solvents 12000mg/L；Trace element solution 2mL/L.
The trace element solution is configured by the following method：FeSO is added in 1L distilled water4·7H2O5g；CoCl2·6H2O50mg；H3BO350mg；(NH4)6Mo7O24·4H2O1.1g；CuSO4·5H2O1.6g；KI10mg；MnCl2·4H2O4g；EDTA50g, stirring shakes up.
Embodiment 1
SBR system is run under aerobic/anaerobic operating mode, instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 3h precipitate 1h, draining stands 1.5h.DO concentration is maintained at DO concentration in 2 ~ 4mg/L, anaerobic stages water and, less than 0.2mg/L, controls 20 ~ 25 DEG C of coolant-temperature gage in aerobic stage water, and adjustment pH value is 7~8.Run 20d.Ammonia nitrogen removal frank reaches that 90%, TP clearance reaches 70%；
Changing system operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.DO concentration, water temperature remain unchanged in aerobic, anaerobic stages water, irregular spoil disposal, and sludge concentration is 4000 ~ 5000mg/L.Run 7d.Ammonia nitrogen removal frank reaches that 70%, TP clearance reaches 66%.
From the activated sludge of reactor, Denitrifying Phosphate Accumulating Organisms are separated using the obligate culture medium of polyP bacteria.By the Dell Ford Pseudomonas filtered out and pseudomonas according to 4:1 ratio compounding, inoculum concentration is 7%, and inoculation liquid is 35% with reactor volume ratio.System operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.Aerobic, anaerobic stages DO concentration, water temperature remain unchanged.Run 7d.Its dephosphorizing rate is up to 85%, and denitrification percent is 85%.If inoculum concentration is 10%, other conditions are constant.Its dephosphorization, denitrification percent change be not obvious.
After testing, TN, TP concentration are respectively contained by water outlet：7.5mg/L、0.5mg/L.Water outlet reaches《Urban wastewater treatment firm pollutant emission standard》（GB18918-2002）One-level A standards.
Embodiment 2
SBR system is run under aerobic/anaerobic operating mode, instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 3h precipitate 1h, draining stands 1.5h.DO concentration is maintained at DO concentration in 2 ~ 4mg/L, anaerobic stages water and, less than 0.2mg/L, controls 20 ~ 25 DEG C of coolant-temperature gage in aerobic stage water, and adjustment pH value is 7~8.Run 30d.Ammonia nitrogen removal frank reaches that 99%, TP clearance reaches 80%.Changing system operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.DO concentration, water temperature remain unchanged in aerobic, anaerobic stages water, irregular spoil disposal, and sludge concentration is 4000 ~ 5000mg/L.Run 10d.Ammonia nitrogen removal frank reaches that 88%, TP clearance reaches 80%.From the activated sludge of reactor, Denitrifying Phosphate Accumulating Organisms are separated using the obligate culture medium of polyP bacteria.By the Dell Ford Pseudomonas filtered out and pseudomonas according to 2:1 ratio compounding, inoculum concentration is 7%, and inoculation liquid is 35% with reactor volume ratio.System operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.Aerobic, anaerobic stages DO concentration, water temperature remain unchanged.Run 10d.Its dephosphorizing rate is up to 96%, and denitrification percent is 91%.If inoculum concentration is 10%, other conditions are constant.Its dephosphorization, denitrification percent change be not obvious.
After testing, TN, TP concentration are respectively contained by water outlet：4.5mg/L、0.2mg/L.Water outlet reaches《Urban wastewater treatment firm pollutant emission standard》（GB18918-2002）One-level A standards.
Embodiment 3
SBR system is run under aerobic/anaerobic operating mode, instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 3h precipitate 1h, draining stands 1.5h.DO concentration is maintained at DO concentration in 2 ~ 4mg/L, anaerobic stages water and, less than 0.2mg/L, controls 20 ~ 25 DEG C of coolant-temperature gage in aerobic stage water, and adjustment pH value is 7~8.Run 25d.Ammonia nitrogen removal frank reaches that 95%, TP clearance reaches 78%.
Changing system operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.DO concentration, water temperature remain unchanged in aerobic, anaerobic stages water, irregular spoil disposal, and sludge concentration is 4000 ~ 5000mg/L.Run 8d.Ammonia nitrogen removal frank reaches that 85%, TP clearance reaches 80%.
By the Dell Ford Pseudomonas filtered out and pseudomonas according to 1:4 ratio compounding, inoculum concentration is 7%, and inoculation liquid is 35% with reactor volume ratio.System operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h.Aerobic, anaerobic stages DO concentration, water temperature remain unchanged.Run 8d.Its dephosphorizing rate is up to 90%, and denitrification percent is 90%.
After testing, TN, TP concentration are respectively contained by water outlet：5mg/L、0.5mg/L.Water outlet reaches《Urban wastewater treatment firm pollutant emission standard》（GB18918-2002）One-level A standards.
Claims (5)
1. a kind of synchronous denitrification dephosphorizing SBR quick start methods, it is characterised in that use sequencing batch reactor SBR, follow the steps below：
（1）SBR system is run under aerobic/anaerobic alternation condition, domestication enrichment polyP bacteria makes its aerobic phosphorus absorption, anaerobic phosphorus release, the phosphate gone by spoil disposal in water removal；
Specially：SBR system is run under aerobic/anaerobic operating mode, instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 3h precipitate 1h, draining stands 1.5h；DO concentration is less than 0.2mg/L in anaerobic stages water, DO concentration is maintained at 2 ~ 4mg/L in aerobic stage water, DO concentration is less than 0.2mg/L in anaerobic stages water, control 20 ~ 25 DEG C of coolant-temperature gage, it is 7~8 to adjust pH value, runs 20 ~ 30d, and ammonia nitrogen removal frank reaches 90% ~ 99%, TP clearance reaches 70% ~ 80%, and polyP bacteria is enriched with；
（2）Change system operation mode, gathering denitrifying polyP bacteria increase anoxic section shortens aeration time, Denitrifying Phosphate Accumulating Organisms inhale phosphorus using intracellular carbon source and by electron acceptor of nitrate nitrogen/nitrite nitrogen；
Specially：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h, DO concentration, water temperature and step in aerobic, anaerobic stages water（1）It is identical, NO in control anaerobic environment3 -Concentration is 30 ~ 40mg/L, and irregular spoil disposal, sludge concentration is 4000 ~ 5000mg/L；7 ~ 10d is run, ammonia nitrogen removal frank reaches that 70% ~ 88%, TP clearance reaches 66% ~ 80%, and Effective multiplication Denitrifying Phosphate Accumulating Organisms suppress the growth of aerobic phosphorous accumulating bacterium and denitrifying bacterium；
（3）Add composite bacteria agent to reactor, make it is biological reinforced with aerobic/anaerobic/anoxic/it is aerobic couple repeatedly, Effective multiplication DPB suppresses fusca xylanase, shortens the reactor start-up time, raising outlet effect,
Specially：From the activated sludge of reactor, Denitrifying Phosphate Accumulating Organisms, i.e. Dell's Ford Pseudomonas and pseudomonas are separated using the obligate culture medium of polyP bacteria, by the Dell Ford Pseudomonas filtered out and pseudomonas by volume（0.25~4）：1 ratio compounding, inoculum concentration is 7 ~ 10% by volume, and inoculation liquid is 35% with reactor volume ratio；System operation mode is：Instant water coming-in 5min, anaerobism stirring 2.5h, aerobic continuous aeration 1.5h, anoxia stirring 1.5h, aerobic aeration 0.5h, precipitates 1h, draining is left unused 1h；Aerobic, anaerobic stages DO concentration, water temperature are identical with step 1, run 7 ~ 10d, its dephosphorizing rate is up to 90% ~ 96%, and denitrification percent is 85 ~ 91%, water outlet after testing《Urban wastewater treatment firm pollutant emission standard》(GB18918-2002) one-level A standards.
2. a kind of synchronous denitrification dephosphorizing SBR quick start methods according to claim 1, it is characterised in that methods described is suitable for the processing of middle low concentration Small Town Wastewater, i.e. raw water COD mass concentrations 200 ~ 350 mg/L, NH4 +The mg/L of 30 ~ 60 mg/L, TP mass concentration of-N mass concentrations 3 ~ 7.
3. a kind of synchronous denitrification dephosphorizing SBR quick start methods according to claim 1, it is characterised in that the method that the obligate culture medium of polyP bacteria separates Denitrifying Phosphate Accumulating Organisms, are followed the steps below：Take the activated sludge 10mL in anaerobism section latter stage in sbr reactor device to be put into the triangular flask equipped with 90mL sterilized waters and bead, sludge is fully shaken up using oscillator and smashed, the sludge solutions after smashing dilute 10 successively-1~10-8Times, the bacterium solution after 0.2mL dilutions is pipetted respectively with pipette to enter in a diameter of 9cm culture dish, obligate 10 ~ the 15mL of culture medium of polyP bacteria is poured into again to be well mixed, after culture dish is inverted in into 30 DEG C of incubated 3d in incubator after culture medium solidifying, the obvious bacterium colony of picking form carries out line separation in culture medium, the Dell Ford Pseudomonas filtered out and pseudomonas.
4. the method that the obligate culture medium of polyP bacteria according to claim 3 separates Denitrifying Phosphate Accumulating Organisms, it is characterised in that the obligate culture medium chemical composition of polyP bacteria is by mass percentage：CH3COONa·3H2O3680mg/L；Na2HPO4·2H2O28.73mg/L；NH4Cl57.27mg/L；MgSO4·7H2O131.82mg/L；K2SO426.74mg/L；CaCl2·2H2O17.2mg/L；Agar 15000mg/L；HEPES buffer solvents 12000mg/L；Trace element solution 2mL/L.
5. the method that the obligate culture medium of polyP bacteria according to claim 4 separates Denitrifying Phosphate Accumulating Organisms, it is characterised in that the trace element solution is configured by the following method：FeSO is added in 1L distilled water4·7H2O5g；CoCl2·6H2O50mg；H3BO350mg；(NH4)6Mo7O24·4H2O1.1g；CuSO4·5H2O1.6g；KI10mg；MnCl2·4H2O4g；EDTA50g, stirring shakes up.
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