CN114230024A - Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency - Google Patents
Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency Download PDFInfo
- Publication number
- CN114230024A CN114230024A CN202111646208.7A CN202111646208A CN114230024A CN 114230024 A CN114230024 A CN 114230024A CN 202111646208 A CN202111646208 A CN 202111646208A CN 114230024 A CN114230024 A CN 114230024A
- Authority
- CN
- China
- Prior art keywords
- phosphorus removal
- biological phosphorus
- bacteria
- removal efficiency
- selectively inhibiting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
Abstract
The invention discloses a method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency, belonging to the field of biological phosphorus removal of sewage; according to the invention, a specific inhibitor aiming at fumarate reductase/succinate dehydrogenase is added into the water inlet or the anaerobic section of the sewage biological treatment system, so that the carbon source intake of the glycan bacteria is selectively inhibited, the proliferation of the glycan bacteria in the biological phosphorus removal system is effectively suppressed, the abundance of the polyphosphate accumulating bacteria, the biological phosphorus removal capability of the system and the effective utilization rate of the carbon source are improved, and the biological phosphorus removal efficiency in the sewage treatment process is improved. The method has the advantages of simple operation, high operability, stability, effectiveness, low cost and the like, is not limited by the quality of inlet water and a sewage treatment process, and has broad-spectrum applicability; in addition, the invention can also be used for high-efficiency and rapid enrichment of phosphorus accumulating bacteria in a laboratory system.
Description
Technical Field
The invention relates to the field of biological phosphorus removal of sewage, in particular to a method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency.
Background
Phosphorus is a main factor causing eutrophication of water bodies, and the over-high concentration of phosphorus in the water bodies can cause mass propagation of algae, destroy the normal ecological balance of the water bodies and influence the use functions of the water bodies and the health of human beings.
In order to improve the water environment, according to a certain regulation, the pollutant discharge standard of a town sewage treatment plant, wherein the I-grade A standard requires that the total phosphorus concentration of effluent is less than 0.5 mg/L.
The enhanced biological phosphorus removal process has the characteristics of high efficiency and economy, and is widely applied to actual-scale urban sewage treatment plants. The process enriches a large number of microorganisms (phosphorus accumulating bacteria) with a phosphorus removing function through alternate operation of anaerobic and aerobic, and in an anaerobic stage, the phosphorus accumulating bacteria decompose intracellular polyphosphate to generate energy to absorb micromolecular organic matters such as volatile fatty acid and the like in sewage, synthesize the absorbed micromolecular organic matters into intracellular polymers to be stored, and release Phosphate (PO) simultaneously4 3--P) to the outside of the cell; in an anoxic or aerobic stage, the phosphorus accumulating bacteria oxidize and decompose the intracellular carbon source stored during anaerobic process to generate energy to complete cell growth and proliferation and excessively absorb phosphate dissolved in water to synthesize polyphosphate. The system achieves the purpose of dephosphorization in the form of residual sludge discharge.
In the biological phosphorus removal process, a microorganism, namely glycan bacteria, competing with phosphorus accumulating bacteria for a carbon source exists, the glycan bacteria can decompose intracellular glycogen under anaerobic conditions to generate energy to take up the carbon source, but do not contribute to a phosphorus removal process, and the excessive propagation of the glycan bacteria in a sewage treatment system can cause the deterioration of the biological phosphorus removal efficiency.
How to effectively control the proliferation of glycan fungi in a biological phosphorus removal system and realize that carbon source substances are more efficiently utilized by the glycan fungi is a key technical problem for realizing the efficient and stable operation of the biological phosphorus removal system, and the existing glycan fungi inhibition method comprises the following steps: utilizing the difference of the utilization capacity and preference of different carbon sources of different glycan bacteria to alternately use different carbon sources (such as acetic acid and propionic acid) so as to realize the inhibition of the glycan bacteria; controlling excessive proliferation of the glycan fungi by reducing the reaction temperature of the system; the method is effective for enriching the phosphorus accumulating bacteria of a laboratory pilot system by improving the pH value of the anaerobic section, but the regulation of the florae of the phosphorus accumulating bacteria and the glycan bacteria in an actual sewage treatment system is difficult to implement, and the development and construction of a florae regulation method which is more targeted and has higher operability in the actual sewage treatment system is urgently needed.
The inventor researches on carbon source uptake mechanisms of phosphorus-accumulating bacteria and glycan bacteria in an actual sewage treatment system to find that in an actual-scale urban sewage treatment plant, the phosphorus-accumulating bacteria and the glycan bacteria have essential differences on the bioenergetic mechanism of carbon source uptake universally, the glycan bacteria generate proton dynamic potential for carbon source uptake by utilizing a hydrogen ion transmembrane transport process coupled with fumarate reductase in a large amount, the phosphorus-accumulating bacteria hardly generate the proton dynamic potential for carbon source uptake, the fumarate reductase/succinate dehydrogenase inhibitor represented by m-phenamidine pamoate can effectively inhibit the carbon source uptake of the glycan bacteria without influencing the anaerobic carbon source uptake and aerobic phosphorus uptake processes of the phosphorus-accumulating bacteria, shows selective inhibition on the glycan bacteria, and can quickly and effectively inhibit the proliferation of the glycan bacteria by adding the m-phenamidine pamoate into a continuous flow biological phosphorus removal reactor, thereby improving the abundance of the phosphorus-accumulating bacteria and improving the phosphorus removal efficiency and the effective utilization rate of the carbon source.
Compared with the traditional method, the method for adding the fumarate reductase/succinate dehydrogenase specific inhibitor is simple and feasible, and has low cost. Therefore, the invention provides a method for selectively pressing glycan bacteria to improve biological phosphorus removal efficiency by using the enzyme activity specific inhibitor, so that the directional regulation and control of floras of a biological phosphorus removal system are realized, and the biological phosphorus removal effect is improved and stabilized.
Disclosure of Invention
The invention aims to overcome the defects and shortcomings of the prior art and provide a method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency.
The method for selectively inhibiting the glycan bacterial in the biological phosphorus removal system by adding the enzyme activity specificity inhibitor improves the phosphorus removal efficiency and the carbon source utilization rate of the biological phosphorus removal system, can greatly save the carbon source adding cost and realize the efficient and stable phosphorus removal effect of the biological phosphorus removal system.
The invention is realized by the following technical scheme:
a method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency comprises the following steps:
adding an enzyme activity specific inhibitor into the sewage treatment system, selectively inhibiting the carbon source intake of the glycan bacteria, and enabling the glycan bacteria to be continuously and selectively eliminated from the system, thereby playing a role in directionally regulating and controlling the flora composition of the biological phosphorus removal system, realizing the enrichment of the polyphosphate accumulating bacteria in the system and the more effective utilization of the carbon source in the sewage treatment process, and ensuring and realizing the stable phosphorus removal effect.
The enzyme activity specific inhibitor is a chemical agent with selective inhibition effect on fumarate reductase and succinate dehydrogenase, such as octopine pamoate, thiabendazole, phenyloxoethyl thiophene amide and the like.
The sewage treatment system can be a sewage treatment plant with actual scale, a pilot-scale system, a laboratory pilot-scale system and a bioreactor for enriching phosphorus-accumulating bacteria in a laboratory.
The adding position of the enzyme activity specific inhibitor is a water inlet section or an anaerobic tank of the treatment system.
The dosage of the enzyme activity specific inhibitor is 5-300 mu m/L of final concentration.
The adding mode of the enzyme activity specificity inhibitor can be continuous adding or intermittent adding.
The invention can be used for the high-efficiency enrichment of phosphorus accumulating bacteria in a laboratory reactor.
Compared with the traditional method for regulating and controlling the flora of the phosphorus accumulating bacteria and the glycan bacteria of the sewage biological phosphorus removal system, the method at least has the following advantages and effects:
1. the enzyme activity specific inhibitor is utilized to inhibit the carbon source intake of the glycan bacteria, thereby promoting the enrichment of the polyphosphate accumulating bacteria and realizing the quick start and stable operation of the sewage biological phosphorus removal system;
2. the adding of an additional organic carbon source is reduced, the operation cost is reduced, and the material consumption is saved;
3. the method has the advantages of simple and easy implementation, high operability and good effect, and is suitable for biological sewage phosphorus removal systems with different processes and different sewage sources;
4. the invention can also be used for enriching the phosphorus accumulating bacteria in a laboratory reactor.
Drawings
FIG. 1 shows the anaerobic phosphorus release and phosphorus removal results of the reactor before and after the addition of the enzyme activity specific inhibitor in the example of the present invention.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Examples
The process for selectively inhibiting glycan bacteria and improving biological phosphorus removal efficiency by using the enzyme activity specific inhibitor comprises the following steps:
when 100mg/L of acetic acid (calculated as TOC) is used as a carbon source material,
the concentration of the phosphate (measured as P) of the inlet water is 25mg/L,
the anaerobic period lasts for 80min,
the aerobic period lasts for 240min,
the precipitation time is 60min for the reaction,
the retention time of the sludge is 15 days,
the hydraulic retention time is 12 hours,
the pH value of the anaerobic section and the aerobic section is 7.0-7.5,
in a laboratory biological phosphorus removal sequencing batch reactor which contains a large amount of glycan bacteria and has a dissolved oxygen concentration DO of 0.8-1.5mg/L and a poor biological phosphorus removal effect, an intermittent feeding mode is adopted, 30 mu mol/L (final concentration) of fumarate reductase inhibitor-octoctal pamoate is added into water inlet of the reactor every 2 days, other operation conditions of the reactor are kept unchanged, after 20 days, under the premise that the operation conditions such as the concentration of a carbon source of water inlet of the reactor are unchanged, the anaerobic phosphorus release amount of the reactor is increased from 50mg/L to 150mg/L, the effective utilization rate of the carbon source is increased by 3 times, the phosphate removal rate is increased from 70% to 100%, and the abundance of the polyphosphate accumulating bacteria is greatly increased (as shown in figure 1).
According to the invention, by adding the enzyme activity specificity inhibitor, the carbon source intake of the glycan strain is selectively inhibited, the enrichment of the phosphorus accumulating strain is promoted, meanwhile, the anaerobic carbon source intake and aerobic phosphorus uptake of the phosphorus accumulating strain are not influenced, the rapid improvement of the biological phosphorus removal activity is realized, and compared with other methods, the method has the characteristics of strong operability, simplicity, practicability, stability and high efficiency.
As described above, the present invention can be preferably realized.
The embodiments of the present invention are not limited to the above-described embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and they are included in the scope of the present invention.
Claims (6)
1. A method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency is characterized by comprising the following steps:
adding an enzyme activity specific inhibitor into the sewage treatment system, selectively inhibiting the carbon source intake of the glycan fungi, and continuously and selectively eliminating the glycan fungi out of the sewage treatment system, thereby playing a role in directionally regulating and controlling the flora composition of the biological phosphorus removal system.
2. The method for selectively inhibiting polysaccharomycetes to improve biological phosphorus removal efficiency according to claim 1, wherein the method comprises the following steps:
the enzyme activity specific inhibitor is a specific inhibitor aiming at bacterial fumarate reductase/succinate dehydrogenase, and is one or the combination of more than two of pamoic acid october, thiabendazole or phenyl oxoethyl thiophene amide.
3. The method for selectively inhibiting polysaccharomycetes to improve biological phosphorus removal efficiency according to claim 2, wherein:
the sewage treatment system is one of a sewage treatment plant, a pilot-scale system, a laboratory system and a bioreactor for enriching phosphorus-accumulating bacteria in a laboratory.
4. The method for selectively inhibiting polysaccharomycetes to improve biological phosphorus removal efficiency according to claim 3, wherein:
the adding position of the enzyme activity specific inhibitor is a water inlet section or an anaerobic tank of the sewage treatment system.
5. The method for selectively inhibiting polysaccharomycetes to improve biological phosphorus removal efficiency according to claim 4, wherein the method comprises the following steps:
the dosage of the enzyme activity specific inhibitor is 5-300 mu m/L of final concentration.
6. The method for selectively inhibiting polysaccharomycetes to improve biological phosphorus removal efficiency of claim 5, wherein:
the adding mode of the enzyme activity specificity inhibitor is continuous adding or intermittent adding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111646208.7A CN114230024A (en) | 2021-12-29 | 2021-12-29 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111646208.7A CN114230024A (en) | 2021-12-29 | 2021-12-29 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114230024A true CN114230024A (en) | 2022-03-25 |
Family
ID=80744496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111646208.7A Pending CN114230024A (en) | 2021-12-29 | 2021-12-29 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114230024A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116395849A (en) * | 2023-04-11 | 2023-07-07 | 深圳市水务(集团)有限公司 | Carbon source adding method for quick start of AOA process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001046459A1 (en) * | 1999-12-23 | 2001-06-28 | Crc For Waste Management And Pollution Control Limited | Probes and primers for the detection of polyphosphate accumulating organisms in wastewater |
| US20110132837A1 (en) * | 2009-12-07 | 2011-06-09 | Ch2M Hill, Inc. | Method and System for Treating Wastewater |
| CN103588300A (en) * | 2013-10-25 | 2014-02-19 | 沈阳建筑大学 | Quick starting method of SBR (Sequencing Batch Reactor) for synchronously denitrifying and removing phosphor |
| CN110436643A (en) * | 2019-08-15 | 2019-11-12 | 北京工业大学 | A kind of control method of side enrichment fusca xylanase |
-
2021
- 2021-12-29 CN CN202111646208.7A patent/CN114230024A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001046459A1 (en) * | 1999-12-23 | 2001-06-28 | Crc For Waste Management And Pollution Control Limited | Probes and primers for the detection of polyphosphate accumulating organisms in wastewater |
| US20110132837A1 (en) * | 2009-12-07 | 2011-06-09 | Ch2M Hill, Inc. | Method and System for Treating Wastewater |
| CN103588300A (en) * | 2013-10-25 | 2014-02-19 | 沈阳建筑大学 | Quick starting method of SBR (Sequencing Batch Reactor) for synchronously denitrifying and removing phosphor |
| CN110436643A (en) * | 2019-08-15 | 2019-11-12 | 北京工业大学 | A kind of control method of side enrichment fusca xylanase |
Non-Patent Citations (4)
| Title |
|---|
| 仇是胜等: "琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅰ)", 《现代农药》 * |
| 徐伟锋等: "生物除磷系统中聚糖菌代谢机理的研究进展", 《微生物学通报》 * |
| 第2期: ""Proton motive force generation from stored polymers for the uptake of acetate under anaerobic conditions"", 《FEMS MICROBIOLOGY LETTERS》 * |
| 邱立平等: "强化生物除磷系统中聚磷菌与聚糖菌的种群分析及代谢机制研究进展", 《环境污染与防治》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116395849A (en) * | 2023-04-11 | 2023-07-07 | 深圳市水务(集团)有限公司 | Carbon source adding method for quick start of AOA process |
| CN116395849B (en) * | 2023-04-11 | 2024-01-12 | 深圳市水务(集团)有限公司 | Carbon source adding method for quick start of AOA process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shi et al. | Achieving partial denitrification using carbon sources in domestic wastewater with waste-activated sludge as inoculum | |
| Xing et al. | pH control and microbial community analysis with HCl or CO2 addition in H2-based autotrophic denitrification | |
| CN102041291B (en) | Method for screening denitrifying bacterium | |
| CN1354143A (en) | Process for removing nitrogen and phosphorus at same time from waste water | |
| Yang et al. | Research progress and application prospect of anaerobic biological phosphorus removal | |
| Liu et al. | Superior nitrogen removal and sludge reduction in a suspended sludge system with in-situ enriching anammox bacteria for real sewage treatment | |
| Sabba et al. | Impact of operational strategies on a sidestream enhanced biological phosphorus removal (S2EBPR) reactor in a carbon limited wastewater plant | |
| CN114410535B (en) | Composite microbial agent, method for preparing microbial composite carbon source and application of microbial composite carbon source in sewage treatment | |
| CN108383239B (en) | Integrated biological treatment process for shortcut nitrification anaerobic ammonia oxidation and phosphorus removal under intermittent aeration mode | |
| CN114772727A (en) | Device and method for synchronously treating domestic sewage and nitrate wastewater based on sulfur autotrophic short-cut denitrification and anaerobic ammonia oxidation technologies | |
| Wu et al. | Characteristics of NO2--N accumulation in partial denitrification during granular sludge formation | |
| CN114230024A (en) | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency | |
| CN112093890B (en) | Method for treating sewage by short-cut nitrification | |
| Zhou et al. | Enhancing nitrogen removal from anaerobically-digested swine wastewater through integration of Myriophyllum aquaticum and free nitrous acid-based technology in a constructed wetland | |
| CN101734790B (en) | Water treatment method for controlling nitrogen and phosphorus contents and reducing sludge by using ozone coupling ASBR/SBR, and reactor | |
| Zhang et al. | Efficient treatment of digested piggery wastewater via an improved anoxic/aerobic process with Myriophyllum spicatum and bionic aquatic weed | |
| Magrí et al. | Batch treatment of liquid fraction of pig slurry by intermittent aeration: process simulation and microbial community analysis | |
| CN212425605U (en) | Deodorant sewage treatment system | |
| CN113830887A (en) | Method for promoting short-cut nitrification to quickly start and improve sludge settling performance by exogenous N-butyryl-L-homoserine lactone | |
| CN111099722B (en) | Composition for promoting denitrification and application thereof | |
| CN114380390A (en) | Culture domestication method for activated sludge of PACT (Picture archiving and communication technology) device | |
| CN201809250U (en) | Novel biological treatment removal tank for total nitrogen in landfill leachate | |
| CN105731619B (en) | The processing method of nitrogen fertilizer production waste water | |
| CN1522973A (en) | Alcohol type fermenting organism desulfurization method for treating sulfate organic wastewater | |
| Primasari et al. | Effects of different pre-treatment methods on anaerobic mixed microflora for hydrogen production and COD reduction from domestic effluent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |