CN110436643A - A kind of control method of side enrichment fusca xylanase - Google Patents
A kind of control method of side enrichment fusca xylanase Download PDFInfo
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- CN110436643A CN110436643A CN201910752066.9A CN201910752066A CN110436643A CN 110436643 A CN110436643 A CN 110436643A CN 201910752066 A CN201910752066 A CN 201910752066A CN 110436643 A CN110436643 A CN 110436643A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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Abstract
A kind of control method of side enrichment fusca xylanase, belongs to field of biological sewage treatment.It is discharged into side the mud mixture amount handled in sequencing batch reactor by controlling the first sequencing batch reactor, realizes that the first sequencing batch reactor operates normally and control the processing load of side treatment reactor；Hydraulic detention time and the side processing time of sequencing batch reactor are handled by control side to control the concentration effect of fusca xylanase；The enrichment degree of fusca xylanase in the first sequencing batch reactor is controlled by control side number of processes.It is pumped into when side processing sequencing batch reactor operation with water distribution not phosphorous in water tank, carbon source is added by carbon source doser, anaerobism end is pumped into water distribution after rich phosphorus supernatant is discharged again, into aerobic stage, to be enriched with fusca xylanase, inhibits polyP bacteria.After reprocessing multiple periods, sludge is pumped into the first sequencing batch reactor again, completes primary complete side processing.Side processing is repeated, realizes the enrichment of fusca xylanase in main reactor.
Description
Technical field
The present invention relates to a kind of control methods of side enrichment fusca xylanase, belong to technical field of biological sewage treatment.
Background technique
PolyP bacteria is a kind of bacterium for being widely present in sewage treatment plant, decomposes intracorporal polyphosphoric acids in anaerobism section
Salt discharges phosphorus in the form of inorganic phosphate, while absorbing the VFA in sewage and entering the internal PHA that formed as interior carbon
Source stores.Under aerobic condition, polyP bacteria decomposes intracorporal PHA and provides energy, phosphorus is excessively inhaled from water body, to realize
The function of dephosphorization.As people deepen constantly microbiologic population's understanding, discovery removes under the conditions of anaerobism, aerobic alternate
There are also a kind of microorganisms to be also enriched with for polyP bacteria, it is exactly fusca xylanase, and fusca xylanase is not in addition to releasing phosphorus and inhaling the function of phosphorus
Can be outer, its metabolism and polyP bacteria is substantially similar, and the internal carbon source that stores in anaerobism section of fusca xylanase is in addition to can be in aerobic rank
Section is for that can also carry out endogenous denitrification in anoxic phases, to realize the effect of denitrogenation outside own growth and development.
Most of in research at this stage is all to have ignored the effect of fusca xylanase to be enriched with based on polyP bacteria, but return
Because how quickly, being effectively enriched with fusca xylanase and also having very important significance in the unique effect of fusca xylanase endogenous denitrification.In
Demonstrating in previous studies can by the method for adjusting water inlet C/P, change types carbon sources, conditioning of mud age, adjusting temperature
Realize the enrichment of fusca xylanase, still, control condition is not detailed, is enriched with and unstable.Also research is discharged by anaerobism section
The enrichment that not phosphorous water distribution enters back into the fusca xylanase of aerobic stage realization is pumped into after rich phosphorus supernatant, but this method can hinder
Hinder being normally carried out for reaction, other microbiologic populations in reactor are impacted, and long processing period, after effect destruction
It needs to reprocess, there are great difficulties for practical application.Therefore, enrichment fusca xylanase is handled by side to realize the depth of main reactor
Degree denitrogenation has very important significance.
Summary of the invention
The invention proposes a kind of control method of side enrichment fusca xylanase, by side handle a large amount of enrichment fusca xylanases from
And the enrichment of fusca xylanase in main reactor may be implemented, the reaction process and population structure for not destroying main reactor the case where
Under, it is suppressed that polyP bacteria solves the problems, such as that traditional wastewater treatment plant anaerobic stages polyP bacteria and fusca xylanase compete carbon source, so that
Fusca xylanase can store a large amount of internal carbon source to endogenous denitrification.
The purpose of the present invention is by the following technical programs to solve: a kind of control method of side enrichment fusca xylanase,
It is characterized by:
Equipment therefor includes the first sequencing batch reactor (1), and side handles sequencing batch reactor (2), matches water tank (3)；
First sequencing batch reactor (1) be equipped with agitating device I (1.1), air compressor I (1.2), spinner flowmeter I (1.3),
Aeration plate I (1.4), DO/pH on-line determination instrument I (1.5), Mud-intake valve I (1.6), mud valve I (1.7), drain valve I (1.8)；Institute
It states side processing sequencing batch reactor (2) and is equipped with agitating device II (2.2), air compressor II (2.3), spinner flowmeter II
(2.4), aeration plate II (2.5), DO/pH on-line determination instrument II (2.6), drain valve II (2.7), inlet valve I (2.8), mud valve
II (2.9), Mud-intake valve II (2.10), automatic medicine feeder (2.11)；Described match water tank (3) are closed case, are equipped with overflow pipe
II (3.1) and blow valve II (3.2)；
First sequencing batch reactor (1) is connect by mud valve I (1.7) with side processing sequencing batch reactor (2)；
It is connected by water pump II (3.3) with side processing sequencing batch reactor (2) with water tank (3)；Side handles batch-type reaction
Device (2) is connected by water pump I (2.1) with the first sequence written instructions reactor (1).
2. the method that application such as described device realizes fusca xylanase enrichment, which is characterized in that specific starting and regulation step are such as
Under:
1) system start-up phase:
First sequencing batch reactor (1) seed sludge is municipal sewage plant A2/ O technique whole process sludge, makes the first sequence
The interior sludge concentration MLSS=3000-3500mg/L of batch reactor (1)；
2) operation phase:
2.1) after the first sequencing batch reactor (1) water inlet mixing, the muddy water of 25%-30% is discharged by mud valve I (1.8)
Mixture to side handles sequencing batch reactor, and the first anaerobism of subsequent first sequencing batch reactor (1) stirs 90-150min, then,
150~180min of aerobic aeration, finally, starting anoxia stirring, time 150-240min, then the total 30min of sedimentation and drawing, is arranged
Water ratio is 50%, and leave unused 30min after draining, starts next period；
2.2) side processing sequencing batch reactor (2) first precipitates 30min, and supernatant is discharged, with not phosphorous in water tank (3)
Water distribution, by intake pump II (3.3) enter side processing sequencing batch reactor (2) in, by automatic medicine feeder add carbon source,
Anaerobism stirring is started simultaneously at, than being 50%, anaerobism stirs 90-150min for water inlet；Then aerobic aeration 60-90min passes through gas
Spinner flowmeter II (2.4) adjusts aeration quantity, and control DO concentration is maintained at 0.8~1.2mg/L, the total 30min of sedimentation and drawing, drains
Than being 50%, a cycle is completed, next period is then begun to；
2.3) the first sequencing batch reactor (1) discharge mud mixture per treatment is that the first sequencing batch reactor (1) has
Imitate the 20%-30% of volume；The hydraulic detention time (HRT) that side handles sequencing batch reactor (2) is controlled in 3h-4h, daily
6-8 period is run, by whole the first sequencing batch reactors of mud mixture blowback (1) after processing 3-6 days, is completed primary complete
Side processing；After completing 2-3 complete side processing, side handles sequencing batch reactor (2) and enters the idle stage；It is idle
After 60-90 days, above-mentioned complete side treatment process is repeated；
Solution used in automatic medicine feeder is the glucose solution of 50g/L, and COD is 150mg/L- after guaranteeing dosing mixing
180mg/L。
The invention patent has the advantage that
1), side processing is enriched with fusca xylanase in the case where not destroying the reaction process and population structure of main reactor,
PolyP bacteria is inhibited, solves the problems, such as that traditional wastewater treatment plant anaerobic stages polyP bacteria and fusca xylanase compete carbon source；
2), side processing inhibits the suction phosphorus of polyP bacteria in aerobic stage without phosphorous water distribution containing carbon source by being added
Process, so as to selective enrichment fusca xylanase；
3) method for, being compared to other enrichment fusca xylanases, side processing enrichment fusca xylanase easily facilitate operation and management,
There is great importance to practical engineering application.
Detailed description of the invention
Fig. 1 is: a kind of control method of side enrichment fusca xylanase.
--- the first sequence makes comments and instructions reactor, 2 --- side handles sequencing batch reactor (2), 3 --- water distribution water in Fig. 1: 1
Case；1.1 --- agitating device I, 1.2 --- air compressor I, 1.3 --- spinner flowmeter I, 1.4 --- aeration plate I,
1.5 --- --- Mud-intake valve I, 1.7 --- mud valve I, 1.8 --- drain valves I of pH and DO on-line determination instrument I, 1.6；2.1——
--- agitating device II, 2.3 --- the air compressor II, 2.4 --- spinner flowmeter II, 2.5-aeration plates of intake pump I, 2.2
II, 2.6 --- --- drain valve II, 2.9 --- mud valve II, 2.10 --- Mud-intake valves of pH and DO on-line determination instrument II, 2.7
II, 2.11 --- automatic medicine feeder；3.1 --- water distribution water tank overflow pipes, 3.2 --- match water tank blow valve
Specific embodiment
The embodiment that the present invention will be described in detail with reference to the accompanying drawings and examples:
Equipment therefor includes the first sequencing batch reactor (1), and side handles sequencing batch reactor (2), matches water tank (3)；
First sequencing batch reactor (1) be equipped with agitating device I (1.1), air compressor I (1.2), spinner flowmeter I (1.3),
Aeration plate I (1.4), DO/pH on-line determination instrument I (1.5), Mud-intake valve I (1.6), mud valve I (1.7), drain valve I (1.8)；Institute
It states side processing sequencing batch reactor (2) and is equipped with agitating device II (2.2), air compressor II (2.3), spinner flowmeter II
(2.4), aeration plate II (2.5), DO/pH on-line determination instrument II (2.6), drain valve II (2.7), inlet valve I (2.8), mud valve
II (2.9), Mud-intake valve II (2.10), automatic medicine feeder (2.11)；Described match water tank (3) are closed case, are equipped with overflow pipe
II (3.1) and blow valve II (3.2)；
First sequencing batch reactor (1) is connect by mud valve I (1.7) with side processing sequencing batch reactor (2)；
It is connected by water pump II (3.3) with side processing sequencing batch reactor (2) with water tank (3)；Side handles batch-type reaction
Device (2) is connected by water pump I (2.1) with the first sequence written instructions reactor (1).
2. the method that application such as described device realizes fusca xylanase enrichment, which is characterized in that specific starting and regulation step are such as
Under:
1) system start-up phase:
First sequencing batch reactor (1) seed sludge is municipal sewage plant A2/O technique whole process sludge, makes reactor
Interior sludge concentration MLSS=3000-3500mg/L.
3) operation phase:
2.1) after the first sequencing batch reactor (1) water inlet mixing, the muddy water of 25%-30% is discharged by mud valve I (1.8)
Mixture to side handles sequencing batch reactor, and the first anaerobism of subsequent first sequencing batch reactor (1) stirs 90-150min, then,
150~180min of aerobic aeration, finally, starting anoxia stirring, time 150-240min, then the total 30min of sedimentation and drawing, is arranged
Water ratio is 50%, and leave unused 30min after draining, starts next period；
2.2) side processing sequencing batch reactor (2) first precipitates 30min, and supernatant is discharged, with not phosphorous in water tank (3)
Water distribution, by intake pump II (3.3) enter side processing sequencing batch reactor (2) in, by automatic medicine feeder add carbon source,
Anaerobism stirring is started simultaneously at, than being 50%, anaerobism stirs 90-150min for water inlet；Then aerobic aeration 60-90min passes through gas
Spinner flowmeter II (2.4) adjusts aeration quantity, and control DO concentration is maintained at 0.8~1.2mg/L, the total 30min of sedimentation and drawing, drains
Than being 50%, a cycle is completed, next period is then begun to.
2.3) the first sequencing batch reactor (1) discharge mud mixture per treatment is the 20%- of reactor effective volume
30%；Side handles hydraulic detention time (HRT) control of sequencing batch reactor (2) in 3h-4h, runs 6-8 period daily,
By whole the first sequencing batch reactors of mud mixture blowback (1) after processing 3-6 days, primary complete side processing is completed.It is complete
After 2-3 complete side processing, side handles sequencing batch reactor (2) and enters the idle stage.After 60-90 days idle, weight
Multiple above-mentioned complete side treatment process.
Solution used in automatic medicine feeder is the glucose solution of 50g/L, and COD is 150mg/L- after guaranteeing dosing mixing
180mg/L。
By monitoring enrichment after activated sludge in phosphorus content, PHA, glycogen variation, it can be found that sludge release phosphorus amount with
And sludge total phosphorus gradually decreases, glycogen and PHA gradually increase, and can release and successfully be enriched with polyP bacteria.
It is specific embodiments of the present invention above, is better understood on and applies this convenient for the those skilled in the art
Invention, but implementation of the invention is without being limited thereto, therefore the simple modifications that the those skilled in the art are the present invention are all
Within that scope of the present invention.
Claims (2)
1. a kind of control method of side enrichment fusca xylanase, it is characterised in that:
Equipment therefor includes the first sequencing batch reactor (1), and side handles sequencing batch reactor (2), matches water tank (3)；It is described
First sequencing batch reactor (1) is equipped with agitating device I (1.1), air compressor I (1.2), spinner flowmeter I (1.3), aeration
Disk I (1.4), DO/pH on-line determination instrument I (1.5), Mud-intake valve I (1.6), mud valve I (1.7), drain valve I (1.8)；The side
Side handle sequencing batch reactor (2) be equipped with agitating device II (2.2), air compressor II (2.3), spinner flowmeter II (2.4),
Aeration plate II (2.5), DO/pH on-line determination instrument II (2.6), drain valve II (2.7), inlet valve I (2.8), mud valve II
(2.9), Mud-intake valve II (2.10), automatic medicine feeder (2.11)；Described match water tank (3) are closed case, are equipped with overflow pipe II
(3.1) and blow valve II (3.2)；
First sequencing batch reactor (1) is connect by mud valve I (1.7) with side processing sequencing batch reactor (2)；Water distribution
Water tank (3) is connected by water pump II (3.3) with side processing sequencing batch reactor (2)；Side handles sequencing batch reactor (2)
It is connected by water pump I (2.1) with the first sequence written instructions reactor (1).
2. applying the method as described in claim 1, which is characterized in that steps are as follows with regulation for specific starting:
1) system start-up phase:
First sequencing batch reactor (1) seed sludge is municipal sewage plant A2/ O technique whole process sludge, keeps the first batch-type anti-
Answer the interior sludge concentration MLSS=3000-3500mg/L of device (1)；
2) operation phase:
2.1) it after the first sequencing batch reactor (1) water inlet mixing, is mixed by the muddy water that 25%-30% is discharged in mud valve I (1.8)
Object to side handles sequencing batch reactor, and the first anaerobism of subsequent first sequencing batch reactor (1) stirs 90-150min, then, aerobic
It is aerated 150~180min, finally, starting anoxia stirring, time 150-240min, then the total 30min of sedimentation and drawing, drains ratio
It is 50%, leave unused 30min after draining, starts next period；
2.2) side processing sequencing batch reactor (2) first precipitates 30min, and supernatant is discharged, and matches with not phosphorous in water tank (3)
Water is entered in side processing sequencing batch reactor (2) by intake pump II (3.3), adds carbon source by automatic medicine feeder, simultaneously
Start anaerobism stirring, than being 50%, anaerobism stirs 90-150min for water inlet；Then aerobic aeration 60-90min passes through Gas rotameters
Flow meter II (2.4) adjusts aeration quantity, and control DO concentration is maintained at 0.8~1.2mg/L, the total 30min of sedimentation and drawing, and draining ratio is
50%, a cycle is completed, next period is then begun to；
2.3) the first sequencing batch reactor (1) discharge mud mixture per treatment is the first sequencing batch reactor (1) effective body
Long-pending 20%-30%；Side handles hydraulic detention time (HRT) control of sequencing batch reactor (2) in 3h-4h, runs daily
6-8 period completes primary complete other by whole the first sequencing batch reactors of mud mixture blowback (1) after processing 3-6 days
Side processing；After completing 2-3 complete side processing, side handles sequencing batch reactor (2) and enters the idle stage；Idle 60-
After 90 days, above-mentioned complete side treatment process is repeated；
Solution used in automatic medicine feeder is the glucose solution of 50g/L, and COD is 150mg/L-180mg/L after guaranteeing dosing mixing.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112093898A (en) * | 2020-07-27 | 2020-12-18 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Rapid culture and enrichment method for glycan fungi |
| CN114230024A (en) * | 2021-12-29 | 2022-03-25 | 华南理工大学 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
| CN115448443A (en) * | 2022-09-25 | 2022-12-09 | 北京工业大学 | Process for enriching glycan bacteria and strengthening low-carbon-nitrogen-ratio domestic sewage deep denitrification by continuous flow AOA (argon oxygen decarburization) side treatment |
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| CN102060413A (en) * | 2010-11-15 | 2011-05-18 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Urban sewage treatment device adopting denitrifying glycogen accumulating organisms (DGAOs) and treatment method thereof |
| CN105906044A (en) * | 2016-06-17 | 2016-08-31 | 北京工业大学 | Device and method for treating low-carbon urban sewage through synchronization of anaerobic ammonia oxidation coupling denitrifying phosphorus removal and endogenous denitrification |
| CN106565016A (en) * | 2016-10-19 | 2017-04-19 | 青岛大学 | Urban sewage nitrogen and phosphorus removal device and method for realizing zero emission of surplus sludge through by-pass flow phosphorous removal |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102060413A (en) * | 2010-11-15 | 2011-05-18 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Urban sewage treatment device adopting denitrifying glycogen accumulating organisms (DGAOs) and treatment method thereof |
| CN105906044A (en) * | 2016-06-17 | 2016-08-31 | 北京工业大学 | Device and method for treating low-carbon urban sewage through synchronization of anaerobic ammonia oxidation coupling denitrifying phosphorus removal and endogenous denitrification |
| CN106565016A (en) * | 2016-10-19 | 2017-04-19 | 青岛大学 | Urban sewage nitrogen and phosphorus removal device and method for realizing zero emission of surplus sludge through by-pass flow phosphorous removal |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112093898A (en) * | 2020-07-27 | 2020-12-18 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Rapid culture and enrichment method for glycan fungi |
| CN114230024A (en) * | 2021-12-29 | 2022-03-25 | 华南理工大学 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
| CN115448443A (en) * | 2022-09-25 | 2022-12-09 | 北京工业大学 | Process for enriching glycan bacteria and strengthening low-carbon-nitrogen-ratio domestic sewage deep denitrification by continuous flow AOA (argon oxygen decarburization) side treatment |
| CN115448443B (en) * | 2022-09-25 | 2023-10-17 | 北京工业大学 | Process for treating enriched polysaccharide bacteria by side of continuous flow AOA to strengthen deep denitrification of low carbon nitrogen ratio domestic sewage |
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