CN110436643B - Control method for laterally enriching glycan bacteria - Google Patents
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Abstract
A control method for laterally enriching glycan bacteria, belonging to the field of sewage biological treatment. The normal operation of the first sequencing batch reactor and the control of the treatment load of the side treatment reactor are realized by controlling the amount of the sludge-water mixture discharged into the side treatment sequencing batch reactor by the first sequencing batch reactor; controlling the enrichment effect of the glycan fungi by controlling the hydraulic retention time and the side treatment time of the side treatment sequencing batch reactor; the enrichment degree of the glycan fungi in the first sequencing batch reactor is controlled by controlling the side treatment times. When the side treatment sequencing batch reactor operates, water without phosphorus is pumped into a water distribution water tank, a carbon source is fed through a carbon source doser, and water is pumped into the water distribution tank again after the anaerobic powder discharges the phosphorus-rich supernatant fluid, and then the aerobic stage is carried out, so that glycan bacteria are enriched and phosphorus accumulating bacteria are inhibited. After repeated treatment for a plurality of cycles, the sludge is pumped into the first sequencing batch reactor again to complete one-time complete side treatment. And repeating side treatment to realize the enrichment of the glycan fungi in the main reactor.
Description
Technical Field
The invention relates to a control method for laterally enriching glycan bacteria, belonging to the technical field of biological sewage treatment.
Background
The phosphorus accumulating bacteria are bacteria widely existing in sewage treatment plants, decompose polyphosphate in vivo in an anaerobic section, release phosphorus in the form of inorganic phosphate, and absorb VFA in sewage to enter the body to form PHA (polyhydroxyalkanoate) which is used as an internal carbon source to be stored. Under aerobic conditions, PHA in the decomposition body of the phosphorus accumulating bacteria provides energy, and excessive phosphorus is absorbed from a water body, so that the function of phosphorus removal is realized. With the continuous deepening of people's understanding of microbial communities, it is found that under the condition of anaerobic and aerobic alternation, a microorganism is enriched besides phosphorus accumulating bacteria, namely glycan bacteria, the glycan bacteria have basically similar metabolism to the phosphorus accumulating bacteria except that the glycan bacteria have no functions of releasing phosphorus and absorbing phosphorus, and an internal carbon source stored in an anaerobic section of the glycan bacteria can be used for self growth and development in an aerobic stage and can also be subjected to endogenous denitrification in an anoxic stage so as to realize the denitrification effect.
Most of the researches at the present stage mainly concentrate the phosphorus-accumulating bacteria and ignore the action of the glycan bacteria, but due to the unique action of endogenous denitrification of the glycan bacteria, how to quickly and effectively enrich the glycan bacteria is of great significance. In previous researches, the glycan bacterium enrichment can be realized by adjusting the C/P of inlet water, changing the type of a carbon source, adjusting the sludge age and adjusting the temperature, but the control conditions are not detailed, and the enrichment is not stable. The enrichment of glycan bacteria is realized by pumping phosphorus-free water distribution after phosphorus-rich supernatant liquid is discharged from an anaerobic stage and then entering an aerobic stage, but the method can hinder the normal operation of the reaction, influences other microbial communities in the reactor, has long treatment period, needs to be retreated after the effect is destroyed, and has great difficulty in practical application. Therefore, the realization of deep denitrification of the main reactor by side treatment of the enriched polysaccharide bacteria has very important significance.
Disclosure of Invention
The invention provides a control method for laterally enriching glycan bacteria, which can realize the enrichment of glycan bacteria in a main reactor by laterally processing a large amount of glycan bacteria, inhibit phosphorus-accumulating bacteria under the condition of not damaging the reaction process and the population structure of the main reactor, solve the problem that the phosphorus-accumulating bacteria and the glycan bacteria compete for a carbon source in the anaerobic stage of a traditional sewage treatment plant, and enable the glycan bacteria to store a large amount of internal carbon sources for endogenous denitrification.
The purpose of the invention is solved by the following technical scheme: a control method for laterally enriching glycan bacteria is characterized by comprising the following steps:
the device comprises a first sequencing batch reactor (1), a side treatment sequencing batch reactor (2) and a water distribution water tank (3); the first sequencing batch reactor (1) is provided with a stirring device I (1.1), an air compressor I (1.2), a rotor flow meter I (1.3), an aeration disc I (1.4), a DO/pH on-line tester I (1.5), a mud inlet valve I (1.6), a mud discharge valve I (1.7) and a drain valve I (1.8); the side treatment sequencing batch reactor (2) is provided with a stirring device II (2.2), an air compressor II (2.3), a rotor flow meter II (2.4), an aeration disc II (2.5), a DO/pH on-line tester II (2.6), a drain valve II (2.7), a water inlet valve I (2.8), a mud discharge valve II (2.9), a mud inlet valve II (2.10) and an automatic doser (2.11); the water distribution water tank (3) is a closed tank body and is provided with an overflow pipe II (3.1) and an emptying valve II (3.2);
the first sequencing batch reactor (1) is connected with the side treatment sequencing batch reactor (2) through a mud valve I (1.7); the water distribution water tank (3) is connected with the side treatment sequencing batch reactor (2) through a water pump II (3.3); the side-treatment sequencing batch reactor (2) is connected with the first sequencing batch reactor (1) through a water pump I (2.1).
2. The method for realizing the enrichment of the glycan fungi by applying the device is characterized by comprising the following specific starting and regulating steps:
1) and (3) a system starting stage:
the first sequencing batch reactor (1) is inoculated with sludge to form a municipal sewage treatment plant A2The whole process of the/O process is sludge, and the sludge concentration MLSS in the first sequencing batch reactor (1) is 3000-3500 mg/L;
2) and (3) an operation stage:
2.1) after water is fed and mixed into the first sequencing batch reactor (1), discharging 25-30% of mud-water mixture to the side treatment sequencing batch reactor through a mud valve I (1.8), then carrying out anaerobic stirring for 90-150min in the first sequencing batch reactor (1), then carrying out aerobic aeration for 150-180 min, finally, starting anoxic stirring for 150-240min, then carrying out precipitation and drainage for 30min, wherein the drainage ratio is 50%, standing for 30min after drainage, and starting the next period;
2.2) the side treatment sequencing batch reactor (2) firstly precipitates for 30min, supernatant is discharged, water without phosphorus in a water distribution tank (3) is distributed into the side treatment sequencing batch reactor (2) through a water inlet pump II (3.3), a carbon source is added through an automatic doser, and anaerobic stirring is started at the same time, wherein the water inlet ratio is 50%, and the anaerobic stirring is carried out for 90-150 min; then aerobic aeration is carried out for 60-90min, aeration quantity is adjusted through a gas rotor flow meter II (2.4), DO concentration is controlled to be kept at 0.8-1.2 mg/L, precipitation and drainage are carried out for 30min totally, the drainage ratio is 50%, one period is completed, and then the next period is started;
2.3) the discharged mud-water mixture treated by the first sequencing batch reactor (1) each time is 20 to 30 percent of the effective volume of the first sequencing batch reactor (1); controlling the Hydraulic Retention Time (HRT) of the side treatment sequencing batch reactor (2) to be 3-4 h, operating for 6-8 cycles every day, and pumping all the sludge-water mixture back to the first sequencing batch reactor (1) after 3-6 days of treatment to complete one-time complete side treatment; after completing the 2-3 times of complete side treatment, the side treatment sequencing batch reactor (2) enters an idle stage; repeating the complete side treatment process after the mixture is idle for 60 to 90 days;
the solution used by the automatic doser is 50g/L glucose solution, and the COD after dosing and mixing is ensured to be 150mg/L-180 mg/L.
The invention has the following advantages:
1) the polysaccharide bacteria are enriched by side treatment, the phosphorus accumulating bacteria are inhibited under the condition that the reaction process and the population structure of the main reactor are not damaged, and the problem that the phosphorus accumulating bacteria and the polysaccharide bacteria compete for a carbon source in the anaerobic stage of the traditional sewage treatment plant is solved;
2) and the side treatment is that water distribution containing carbon source but not containing phosphorus is added, and the phosphorus absorption process of the phosphorus accumulating bacteria is inhibited in an aerobic stage, so that the glycan bacteria can be selectively enriched;
3) compared with other methods for enriching the glycan bacteria, the method for laterally processing the enriched glycan bacteria is more convenient to operate and manage, and has important guiding significance for practical engineering application.
Drawings
FIG. 1 is a schematic diagram of: a control method of a side enrichment glycan bacterium.
In fig. 1: 1-a first sequencing batch reactor, 2-a side treatment sequencing batch reactor (2), 3-a water distribution tank; 1.1-stirring device I, 1.2-air compressor I, 1.3-rotor flow meter I, 1.4-aeration disc I, 1.5-pH and DO online measuring instrument I, 1.6-mud inlet valve I, 1.7-mud discharge valve I, 1.8-water discharge valve I; 2.1-water inlet pump I, 2.2-stirring device II, 2.3-air compressor II, 2.4-rotor flow meter II, 2.5-aeration disc II, 2.6-pH and DO online determinator II, 2.7-water discharge valve II, 2.9-mud discharge valve II, 2.10-mud inlet valve II, 2.11-automatic doser; 3.1-water distribution tank overflow pipe, 3.2-water distribution tank emptying valve
Detailed Description
The embodiments of the present invention will be described in detail below with reference to the accompanying drawings and examples:
the device comprises a first sequencing batch reactor (1), a side treatment sequencing batch reactor (2) and a water distribution water tank (3); the first sequencing batch reactor (1) is provided with a stirring device I (1.1), an air compressor I (1.2), a rotor flow meter I (1.3), an aeration disc I (1.4), a DO/pH on-line tester I (1.5), a mud inlet valve I (1.6), a mud discharge valve I (1.7) and a drain valve I (1.8); the side treatment sequencing batch reactor (2) is provided with a stirring device II (2.2), an air compressor II (2.3), a rotor flow meter II (2.4), an aeration disc II (2.5), a DO/pH on-line tester II (2.6), a drain valve II (2.7), a water inlet valve I (2.8), a mud discharge valve II (2.9), a mud inlet valve II (2.10) and an automatic doser (2.11); the water distribution water tank (3) is a closed tank body and is provided with an overflow pipe II (3.1) and an emptying valve II (3.2);
the first sequencing batch reactor (1) is connected with the side treatment sequencing batch reactor (2) through a mud valve I (1.7); the water distribution water tank (3) is connected with the side treatment sequencing batch reactor (2) through a water pump II (3.3); the side-treatment sequencing batch reactor (2) is connected with the first sequencing batch reactor (1) through a water pump I (2.1).
2. The method for realizing the enrichment of the glycan fungi by applying the device is characterized by comprising the following specific starting and regulating steps:
1) and (3) a system starting stage:
the sludge inoculated in the first sequencing batch reactor (1) is sludge in the whole process of A2/O technology of an urban sewage treatment plant, and the sludge concentration MLSS in the reactor is 3000-3500 mg/L.
3) And (3) an operation stage:
2.1) after water is fed and mixed into the first sequencing batch reactor (1), discharging 25-30% of mud-water mixture to the side treatment sequencing batch reactor through a mud valve I (1.8), then carrying out anaerobic stirring for 90-150min in the first sequencing batch reactor (1), then carrying out aerobic aeration for 150-180 min, finally, starting anoxic stirring for 150-240min, then carrying out precipitation and drainage for 30min, wherein the drainage ratio is 50%, standing for 30min after drainage, and starting the next period;
2.2) the side treatment sequencing batch reactor (2) firstly precipitates for 30min, supernatant is discharged, water without phosphorus in a water distribution tank (3) is distributed into the side treatment sequencing batch reactor (2) through a water inlet pump II (3.3), a carbon source is added through an automatic doser, and anaerobic stirring is started at the same time, wherein the water inlet ratio is 50%, and the anaerobic stirring is carried out for 90-150 min; then aerobic aeration is carried out for 60-90min, aeration quantity is adjusted through a gas rotor flow meter II (2.4), DO concentration is controlled to be kept at 0.8-1.2 mg/L, precipitation and drainage are carried out for 30min totally, the drainage ratio is 50%, one period is completed, and then the next period is started.
2.3) the discharged mud-water mixture treated by the first sequencing batch reactor (1) each time is 20 to 30 percent of the effective volume of the reactor; the Hydraulic Retention Time (HRT) of the side treatment sequencing batch reactor (2) is controlled to be 3h-4h, the operation is carried out for 6-8 cycles every day, and after the treatment is carried out for 3-6 days, all the mud-water mixture is pumped back to the first sequencing batch reactor (1) to complete one-time complete side treatment. After completing the 2-3 times of complete side treatment, the side treatment sequencing batch reactor (2) enters an idle stage. After standing for 60-90 days, the above complete side treatment process is repeated.
The solution used by the automatic doser is 50g/L glucose solution, and the COD after dosing and mixing is ensured to be 150mg/L-180 mg/L.
By monitoring the changes of phosphorus content, PHA and glycogen in the enriched activated sludge, the phosphorus release amount of the sludge and the total phosphorus of the sludge are gradually reduced, and the glycogen and the PHA are gradually increased, so that the successful enrichment of the phosphorus accumulating bacteria can be deduced.
The foregoing is a detailed description of the invention that will enable those skilled in the art to better understand and utilize the invention, and it is not to be limited thereby, since various modifications and changes may be made by those skilled in the art without departing from the scope of the invention.
Claims (1)
1. A control method for side enrichment of glycan bacteria comprises a first sequencing batch reactor (1), a side treatment sequencing batch reactor (2) and a water distribution tank (3); the first sequencing batch reactor (1) is provided with a stirring device I (1.1), an air compressor I (1.2), a rotor flow meter I (1.3), an aeration disc I (1.4), a DO/pH on-line tester I (1.5), a mud inlet valve I (1.6), a mud discharge valve I (1.7) and a drain valve I (1.8); the side treatment sequencing batch reactor (2) is provided with a stirring device II (2.2), an air compressor II (2.3), a rotor flow meter II (2.4), an aeration disc II (2.5), a DO/pH on-line tester II (2.6), a drain valve II (2.7), a water inlet valve I (2.8), a mud discharge valve II (2.9), a mud inlet valve II (2.10) and an automatic doser (2.11); the water distribution water tank (3) is a closed tank body and is provided with an overflow pipe II (3.1) and an emptying valve II (3.2);
the first sequencing batch reactor (1) is connected with the side treatment sequencing batch reactor (2) through a mud valve I (1.7); the water distribution water tank (3) is connected with the side treatment sequencing batch reactor (2) through a water pump II (3.3); the side treatment sequencing batch reactor (2) is connected with the first sequencing batch reactor (1) through a water pump I (2.1);
the method is characterized by comprising the following specific starting and regulating steps:
1) and (3) a system starting stage:
the first sequencing batch reactor (1) is inoculated with sludge to form a municipal sewage treatment plant A2The whole process of the/O process is sludge, and the sludge concentration MLSS in the first sequencing batch reactor (1) is 3000-3500 mg/L;
2) and (3) an operation stage:
2.1) after water is fed and mixed into the first sequencing batch reactor (1), discharging 25-30% of mud-water mixture to the side treatment sequencing batch reactor through a mud valve I (1.7), then carrying out anaerobic stirring for 90-150min in the first sequencing batch reactor (1), then carrying out aerobic aeration for 150-180 min, finally, starting anoxic stirring for 150-240min, then carrying out precipitation and drainage for 30min, wherein the drainage ratio is 50%, standing for 30min after drainage, and starting the next period;
2.2) the side treatment sequencing batch reactor (2) firstly precipitates for 30min, supernatant is discharged, water without phosphorus in a water distribution tank (3) is distributed into the side treatment sequencing batch reactor (2) through a water inlet pump II (3.3), a carbon source is added through an automatic doser, and anaerobic stirring is started at the same time, wherein the water inlet ratio is 50%, and the anaerobic stirring is carried out for 90-150 min; then aerobic aeration is carried out for 60-90min, aeration quantity is adjusted through a gas rotor flow meter II (2.4), DO concentration is controlled to be kept at 0.8-1.2 mg/L, precipitation and drainage are carried out for 30min totally, the drainage ratio is 50%, one period is completed, and then the next period is started;
2.3) the discharged mud-water mixture treated by the first sequencing batch reactor (1) each time is 20 to 30 percent of the effective volume of the first sequencing batch reactor (1); controlling the Hydraulic Retention Time (HRT) of the side treatment sequencing batch reactor (2) to be 3-4 h, operating for 6-8 cycles every day, and pumping all the sludge-water mixture back to the first sequencing batch reactor (1) after 3-6 days of treatment to complete one-time complete side treatment; after completing the 2-3 times of complete side treatment, the side treatment sequencing batch reactor (2) enters an idle stage; repeating the complete side treatment process after the mixture is idle for 60 to 90 days;
the solution used by the automatic doser is 50g/L glucose solution, and the COD after dosing and mixing is ensured to be 150mg/L-180 mg/L.
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| CN114230024A (en) * | 2021-12-29 | 2022-03-25 | 华南理工大学 | Method for selectively inhibiting glycan bacteria to improve biological phosphorus removal efficiency |
| CN115448443B (en) * | 2022-09-25 | 2023-10-17 | 北京工业大学 | Process for treating enriched polysaccharide bacteria by side of continuous flow AOA to strengthen deep denitrification of low carbon nitrogen ratio domestic sewage |
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| CN105906044B (en) * | 2016-06-17 | 2018-12-18 | 北京工业大学 | The device and method of the synchronous endogenous denitrification processing Low-carbon Urban Wastewater of Anammox coupling denitrification dephosphorization |
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