CN114426334B - Rapid enrichment culture method of denitrifying phosphorus removal bacteria - Google Patents
Rapid enrichment culture method of denitrifying phosphorus removal bacteria Download PDFInfo
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Images
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/02—Temperature
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/04—Oxidation reduction potential [ORP]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/42—Liquid level
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention relates to a rapid enrichment culture method of denitrifying phosphorus removal bacteria, which comprises the following steps of (1) strengthening the phosphorus removal performance of sludge: anaerobic culture is firstly carried out, inflection point ORP1 is determined, and water is discharged until the culture is finished; then performing anoxic cultivation, determining inflection point ORP2, draining water until cultivation is finished, and alternately performing the steps, and ending the strengthening process when the denitrifying phosphorus removal bacteria account for more than 20% of all the phosphorus removal bacteria; (2) enrichment culture of denitrifying phosphorus removal bacteria: the ORP1 and the ORP2 are used as switching points of anaerobic culture and anoxic culture for alternate culture, and enrichment culture is completed when the denitrifying phosphorus removal bacteria account for more than 70% of the total phosphorus removal bacteria. According to the invention, a two-step combined culture mode of sludge dephosphorization performance enhancement and denitrification dephosphorization bacteria enrichment culture is adopted, and the precise switching of different stages is realized by combining ORP as a control parameter, so that the rapid enrichment culture of thalli is realized.
Description
Technical Field
The invention belongs to the field of environmental microorganisms, and particularly relates to a rapid enrichment culture method of denitrifying phosphorus removal bacteria.
Background
The problem of eutrophication of the current water body environment is increasingly serious, the national sewage discharge standard is continuously improved, and in order to control the eutrophication of the water body, the sewage treatment technology of denitrification and dephosphorization has become one of the research hot spots in the current sewage treatment field. Compared with the physical and chemical methods, the biological treatment method has the advantages of low cost and small secondary pollution, so the biological denitrification and dephosphorization technology is always the main research object in the field of sewage treatment. The traditional biological nitrogen and phosphorus removal process has a certain effect on biological nitrogen and phosphorus removal of wastewater, but has some problems, such as: the phosphorus removal bacteria and the denitrifying bacteria compete for organic carbon sources; the sludge ages of nitrifying bacteria, denitrifying bacteria and dephosphorizing bacteria are different, and various bacterial groups are mutually restricted by mixing together, so that the system is difficult to reach the optimal running condition; in addition, the system running cost is also generally high.
In recent years, the synchronous denitrification and dephosphorization technology has new application prospect. Denitrifying phosphorus removal bacteria (DPBs) are a process that uses nitrate as an electron acceptor under anoxic conditions to provide energy to excessively adsorb phosphorus using PHA stored in the body under anaerobic conditions. Because it adopts NO 3 - -N replaces O 2 As an electron acceptor, the method can simultaneously remove nitrogen and phosphorus, solves the contradiction problem of denitrifying bacteria and dephosphorizing bacteria on organic carbon sources in the traditional biological denitrification and dephosphorization process (the sludge age of nitrifying bacteria is generally 20-50 days and the sludge age of dephosphorizing bacteria is generally about 15 days), has the advantages of low energy consumption, less residual sludge, less carbon dioxide emission and the like, and is regarded as a sustainable process, and becomes a hot spot for denitrification and dephosphorization research in recent years. The cultivation and domestication of denitrifying phosphorus removal bacteria are the important problems to be solved by the denitrifying phosphorus removal process.
CN201010590494.5 discloses a reaction device for rapidly enriching denitrifying phosphorus removal bacteria, which is an SBR reaction device suitable for rapidly enriching denitrifying phosphorus removal sludge in a denitrifying phosphorus removal process such as Dephanox. The method comprises the following steps: the A/An SBR reactor (1) is respectively connected with a carbon source water inlet pump (2) and a nitrogen and phosphorus source water inlet pump (3), and is arranged in An anaerobic section: pumping the carbon source wastewater into an A/AnSBR reactor (1) by a carbon source water inlet pump (2), reacting under the stirring action of a stirrer (5), and precipitating and draining after the reaction is finished; in the anoxic section: the nitrogen and phosphorus source water inlet pump (3) pumps the nitrogen and phosphorus source wastewater into the A/An SBR reactor (1), and the wastewater is precipitated and drained after the reaction is completed under the stirring action of the stirrer (5). The gas generated in the A/An SBR reactor (1) is discharged from a gas collecting pipe (1-4) at the upper part, and the gas collecting pipe (1-4) is connected with a water seal device (11) through a pipeline. 3 sampling pipes (1-7) are evenly arranged on the side wall of the A/An SBR reactor (1) according to the height, a mud discharging pipe (1-8) is arranged at the lower end of the sampling pipes, an ORP probe mounting pipe (1-9) and a pH probe mounting pipe (1-10) are arranged on the other side wall, and changes of ORP and pH values in the A/An SBR reactor (1) can be monitored on line through the arrangement of An ORP meter and a pH meter. The invention uses a special two-stage water inlet mode to make the device play a role of a biological selector, on one hand, the separation of an electron donor and an electron acceptor can be fully realized, and the competition of other bacterial groups such as denitrifying bacteria and denitrifying dephosphorizing bacteria on the electron donor and the electron acceptor can be avoided, so that the phosphorus accumulating bacteria (mainly DPBs) can obtain a competitive advantage in an anaerobic stage, thereby the phosphorus accumulating bacteria group can be selectively proliferated in the system, on the other hand, the nutrition load of sludge can be improved under the condition of a certain water inlet concentration, and the bacterial proliferation speed can be accelerated.
CN103936153a discloses a rapid screening method of dominant bacteria of phosphorus accumulating granular sludge, comprising (1) adopting simulated urban sewage domestication denitrification dephosphorization bacteria in a sequencing batch reactor to obtain denitrification dephosphorization activated sludge; (2) Rapidly forming denitrification dephosphorization activated sludge formed in the step (1) into denitrification dephosphorization granular sludge in a sequencing batch reactor; (3) And (3) carrying out endogenous starvation reaction on the denitrification dephosphorization granular sludge obtained in the step (2), and screening dominant bacterial phosphorus accumulating bacteria by a rapid sedimentation method. The method can rapidly enrich the phosphorus accumulating bacteria in the laboratory SBR granular sludge technical sample, improve the proportion of the phosphorus accumulating bacteria, control and eliminate the polysaccharide bacteria, control the competition between the phosphorus accumulating bacteria and the polysaccharide bacteria, improve the strain screening efficiency, and provide reliable guarantee for improving and stabilizing the denitrification dephosphorization effect. However, the method needs to run for 90 days in step (1), and the cultivation is relatively long, so that the denitrification dephosphorization process is slowly built.
CN101555068A discloses a method for culturing aerobic granular sludge for simultaneously denitrifying and dephosphorizing domestic sewage at a low temperature, which is characterized in that an SBR reactor adopts a height-diameter ratio of 2-10 and a volume exchange rate of 50-67%, and the water temperature is not regulated. The sedimentation time is 30-50 min, and gradually shortened to 1-3 min; or setting the sedimentation time to 5-8 min, and gradually shortening the sedimentation time to 1-3 min. In the biochemical reaction process, dissolved oxygen concentration DO, oxidation-reduction potential ORP and pH value are used as real-time control parameters, and stirring time (denitrification and phosphorus release) and aeration time (organic oxidation, nitrification, denitrification, aerobic phosphorus absorption and denitrification phosphorus absorption) are controlled in real time. The fuzzy control rule of the stirring time is as follows: firstly, detecting the denitrification ending time according to a fuzzy control rule, namely when the deviation of oxidation-reduction potential ORP reaches negative middle or negative large, the first derivative of ORP reaches negative middle or negative large, and meanwhile, the first derivative of pH reaches negative zero or negative small, and the denitrification is ended at the moment; after denitrification is finished, the first derivative of ORP fluctuates at-5-0 mV/min, and phosphorus discharge is finished at this time, and stirring is stopped.
CN1948184a discloses a synchronous nitrification and denitrification control method and device for an A2/O oxidation ditch process, which monitors a macroscopic aerobic-anoxic environment formed in an oxidation ditch aeration tank through a DO on-line monitor (12), monitors a microscopic aerobic-anoxic environment formed in the oxidation ditch aeration tank through an ORP on-line monitor (13), controls aeration by using a variable frequency blower (14) controlled by a computer (21), and ensures that the nitrification reaction and the denitrification reaction in macroscopic and microscopic states are carried out simultaneously. The invention realizes the removal rate of more than 80% of total nitrogen and the removal rate of more than 90% of ammonia nitrogen in the synchronous nitrification and denitrification of the A2/O oxidation ditch process. Wherein, the indication number of the ORP on-line monitor (13) is controlled within the range of-30 mv to 30 mv.
CN101570383a discloses a deep denitrification and dephosphorization device and a process control method, which combines an inverted A2/O process and a staged water inlet process, does not need to set up internal reflux of nitrified liquid, sets up an anaerobic reactor in a first section, and installs an ORP on-line sensor and a nitrate on-line sensor in a second section and a third section of anoxic reactor respectively, so as to collect ORP and nitrate data on line as input of a process controller, output the data through fuzzification treatment and act on the opening or closing of an actuator frequency converter and an external carbon source dosing metering pump, and save the external carbon source dosage. According to the invention, the nitrate nitrogen in the effluent water of the anoxic zone is controlled to be maintained below 2mg/L through an ORP on-line sensor (20) and a nitrate on-line sensor (21); when the nitrate nitrogen concentration of the effluent of each section of anoxic reactor exceeds the range, an external carbon source dosing metering pump (28) is started, and when the nitrate nitrogen concentration of the effluent is within the range, the external carbon source dosing metering pump (28) is closed, so that the denitrification effect of the segmented water inlet process can be greatly improved, the carbon source of raw water is utilized to the greatest extent, synchronous denitrification and dephosphorization are realized, the control structure is simple, and the effluent quality is stable.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a rapid enrichment culture method of denitrifying phosphorus removal bacteria. According to the invention, a two-step combined culture mode of sludge dephosphorization performance enhancement and denitrification dephosphorization bacteria enrichment culture is adopted, and the precise switching of different stages is realized by combining ORP as a control parameter, so that the rapid enrichment culture of thalli is realized.
The invention provides a rapid enrichment culture method of denitrifying phosphorus removal bacteria, which comprises the following steps:
(1) And (3) strengthening the dephosphorization performance of the sludge: inoculating sludge containing denitrifying phosphorus removal bacteria into a incubator, performing anaerobic culture and determining inflection point ORP1, and draining water until the culture is finished; switching to anoxic cultivation, and determining inflection point ORP2 until drainage is finished; when the denitrifying phosphorus removal bacteria account for more than 20% of all the phosphorus removal bacteria, the strengthening process is finished;
(2) Enrichment culture of denitrifying phosphorus removal bacteria: the ORP1 and the ORP2 are used as switching points of anaerobic culture and anoxic culture for alternate culture, and enrichment culture is completed when the denitrifying phosphorus removal bacteria account for more than 70% of the total phosphorus removal bacteria.
In the method, the sludge containing the denitrifying phosphorus removal bacteria in the step (1) is sludge containing the denitrifying phosphorus removal bacteria accounting for less than 20 percent of the total phosphorus removal bacteria, generally 1 to 15 percent, such as residual sludge in a secondary sedimentation tank of a municipal sewage treatment plant.
In the method, the sludge in the step (1) is inoculated according to the sludge concentration (MLSS) of 3000-5000mg/L after inoculation.
In the method of the invention, the anaerobic culture conditions in the step (1) are as follows: the concentration of dissolved oxygen is lower than 0.1mg/L, the temperature is 20-35 ℃, the pH is 7.0-8.0, and the stirring speed is 50-60r/min.
In the method of the invention, the anoxic culture conditions in the step (1) are as follows: the concentration of dissolved oxygen is 0.1-0.5mg/L, the temperature is 20-35 ℃, the pH is 7.0-8.0, and the stirring speed is 50-60r/min.
In the method, the sludge containing denitrifying phosphorus removal bacteria in the step (1) is inoculated into a reactor, the anaerobic culture adopts a conventional culture medium, preferably a culture solution S1 containing COD and ammonia nitrogen is used for culture, ORP begins to decline along with the progress of the reaction, the ORP takes a rapid decline period and a slow decline period, the inflection point is determined as ORP1, and sedimentation and drainage are carried out until the substrate is almost exhausted after the culture is finished. The anoxic culture adopts a conventional culture medium, preferably adopts a culture solution S2 containing nitrate nitrogen and phosphorus for culture, and the ORP starts to decline as the reaction proceeds, and shows a rapid decline period and a slow decline period, and the inflection point is determined as ORP2; until the culture is finished, the substrate is almost exhausted for sedimentation and drainage. Anaerobic culture and anoxic culture are alternately performed, and when the denitrifying phosphorus removal bacteria account for more than 20% of the total phosphorus removal bacteria, the intensified culture process is ended.
In the method of the invention, in the culture solution S1 containing COD and ammonia nitrogen, the concentration of COD is 100-300mg/L, the concentration of ammonia nitrogen is 3-10mg/L, wherein the substance for providing COD can be at least one of acetic acid, sodium acetate, propionic acid and the like, and the substance for providing ammonia nitrogen can be at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate, ammonium carbonate and the like. Further, the culture solution S1 also comprises CaCl 2 、MgSO 4 And FeSO 4 Wherein CaCl 2 0.02-0.03g/L MgSO 4 0.05-0.06g/L FeSO 4 0.0010-0.0012g/L. Further, the culture solution S1 also comprises a microelement nutrient solution, and the concentration is 1.0-1.5g/L; wherein each liter of the microelement nutrition liquid contains 10g of EDTA and 10g of FeCl 3 •6H 2 O 1.5g、ZnSO 4 •7H 2 O 0.12g、MnCl 2 •4H 2 O 0.12g、H 3 BO 3 0.15g、KI 0.18g、Na 2 MoO 4 •2H 2 O 0.06g、CuSO 4 •5H 2 O 0.03g、CoCl 2 •6H 2 O 0.15g。
In the culture solution S2 containing the nitrate nitrogen and the phosphorus, the concentration of the nitrate nitrogen is 50-70mg/L, and the concentration of the phosphorus is 10-40mg/L, wherein the substance for providing the nitrate nitrogen is mainly nitrate, for example, at least one of potassium nitrate, sodium nitrate, ammonium nitrate and the like, and the substance for providing the P is mainly orthophosphate, for example, at least one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate, disodium hydrogen phosphate and the like. Further, the culture solution S2 also comprises ammonia nitrogen and trace element nutrient solution, wherein the substance for providing ammonia nitrogen can be at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate, ammonium carbonate and the like, and the concentration of the ammonia nitrogen is 3-10mg/L. The microelement nutrition liquid is the same as the culture liquid S1.
In the method, in the step (1), when the denitrifying phosphorus removal bacteria account for more than 20 percent of the total phosphorus removal bacteria, preferably 20 to 30 percent, the sludge phosphorus removal performance strengthening process is completed.
In the invention, the step (2) is used for inoculating the sludge reinforced in the step (1), stopping the reaction when the ORP is reduced to be close to the ORP1, settling, draining water and switching to perform anoxic culture; stopping the reaction when the ORP is reduced to be close to ORP2, settling, draining water, and switching to anaerobic culture; such anaerobic culture and anoxic culture are alternately performed.
In the present invention, when ORP is reduced to near ORP1, preferably ORP-orp1=0 to 10mV in the anaerobic culture in step (2), the reaction is stopped and the anaerobic culture is switched.
In the present invention, when ORP is reduced to near ORP2, preferably ORP-orp2=0 to 10mV in the anoxic cultivation in step (2), the reaction is stopped and the anaerobic cultivation is switched.
In the invention, the operating conditions of anaerobic culture and anoxic culture and the culture solution in the step (2) are the same as those in the step (1).
In the invention, the step (2) is cultivated until enrichment cultivation is completed when the denitrifying phosphorus removal bacteria in the sludge account for more than 70 percent, preferably 70 to 90 percent of the total phosphorus removal bacteria.
Compared with the prior art, the invention has the following beneficial effects:
(1) In the process of culturing thalli, the end point of culture is monitored according to the OD value of thalli, the weight of thalli, the culture time or the concentration of discharged culture solution, and the inventor finds that the anaerobic section and the anoxic section in the reaction process are monitored by adopting ORP in the process of culturing denitrifying phosphorus removal bacteria, and the two culture sections are precisely switched through inflection points ORP1 and ORP2, so that the invalid or low-efficiency culture in the culture process is obviously reduced, the rapid enrichment culture of the denitrifying phosphorus removal bacteria is realized, and the problem of slow starting of an industrial denitrifying phosphorus removal bacteria reactor is solved.
(2) Aiming at the characteristics of denitrifying phosphorus removal bacteria, the sludge containing low-concentration denitrifying phosphorus removal bacteria is subjected to phosphorus removal performance enhancement, then the anaerobic section and the anoxic section are accurately switched by combining the change inflection point of ORP, the control process is accurate and effective, the adverse factors of delaying the culture time are eliminated, and the quick enrichment of the bacteria is realized.
(3) The process control of the method ensures that the two processes of phosphorus release and phosphorus absorption are not affected, provides the best growth conditions for the two process thalli, and realizes the selective rapid proliferation of the thalli in different stages.
Drawings
FIG. 1 is a process flow diagram of the enrichment culture method of the present invention;
wherein, 1-culture solution S1 storage tank, 2-culture solution S2 storage tank, 3-main reactor, 4-feed pump, 5-agitator, 6-ORP appearance, 7-level gauge, 8-drain valve, 9-pH meter.
FIG. 2 is a schematic representation of the locations of OPR1 and ORP2 as determined by the anaerobic and anoxic sections of example 1 of the invention.
Detailed Description
The method and effect of the present invention will be described in detail with reference to examples. The embodiments and specific operation procedures are given on the premise of the technical scheme of the invention, but the protection scope of the invention is not limited to the following embodiments.
The experimental methods in the following examples, unless otherwise specified, are all conventional in the art. The experimental materials used in the examples described below were purchased from biochemical reagent stores unless otherwise specified.
In the embodiment of the invention, the COD concentration is measured by using GB11914-1989 method of measuring the chemical oxygen demand of water quality-potassium dichromate; the nitrate nitrogen concentration is measured by using GB7480-1987 method for measuring Water quality-nitrate nitrogen-phenol disulfonic acid spectrophotometry; the phosphorus concentration was determined using GB11893-1989 Spectrophotometry of ammonium molybdate.
In the invention, the proportion of denitrifying phosphorus removal bacteria in all phosphorus removal bacteria is expressed by the ratio of anoxic phosphorus absorption rate to aerobic phosphorus absorption rate. The sludge is equally divided into two parts, one part is subjected to an aerobic phosphorus absorption experiment (aeration-ae), and the other part is subjected to an anoxic phosphorus absorption experiment (NaNO is added) 3 -an), after the same reaction time t, the P concentration changes of the inlet water and the outlet water are respectively q1 and q2 (mg/L), q ae =q1/t，q an =q2/t. According to the assumption that the denitrifying phosphorus removal bacteria have the same phosphorus absorption rate under the anoxic and aerobic conditions, the proportion of the denitrifying phosphorus removal bacteria in the activated sludge in all the phosphorus removal bacteria under the anaerobic and aerobic culture modes is calculated according to the following formula,
wherein: r is R DPB The percent of denitrifying phosphorus removal bacteria accounts for the proportion of all phosphorus removal bacteria; q an mg/L/h is the anoxic phosphorus absorption rate of the denitrification phosphorus removal bacteria; q ae The aerobic phosphorus absorption rate of all the phosphorus removal bacteria is mg/L/h.
The sludge inoculated in the embodiment is phosphorus-containing residual sludge in a secondary sedimentation tank of a sewage treatment plant, after the phosphorus removal performance of the anaerobic-anoxic process is enhanced, the phosphorus-containing sludge is inoculated into a reactor for enrichment of denitrifying phosphorus removal bacteria, the inoculated MLSS is 4000mg/L, and the denitrifying phosphorus removal bacteria in the sludge at this time account for about 10% of all the phosphorus removal bacteria.
In the invention, the culture reactor is a common complete mixing type bioreactor, as shown in figure 1, and comprises an anaerobic section culture solution S1 storage tank 1, an anoxic section culture solution S2 storage tank 2, a main body reactor 3, feed pumps 4-1 and 4-2, a stirrer 5, an ORP meter 6, a liquid level meter 7 (high liquid level and low liquid level), a drain valve 8 and a pH meter 9, wherein the PLC controls the feed pump, the stirrer, the ORP meter, the liquid level meter and the drain valve. The reaction process is divided into two stages of anaerobic and anoxic, and the operation process of the anaerobic stage is as follows: the running process of the anoxic section comprises the following steps of water inlet, anaerobic, sedimentation and drainage: water inflow, hypoxia, sedimentation and drainage. The anaerobic section water inlet pump 4-1 is started, the culture solution S1 is fed, stirring is started, when the liquid level reaches the high liquid level of the liquid level meter 7, the water inlet pump 4-1 is stopped, the anaerobic phosphorus release process occurs, stirring is stopped after the culture is finished, sedimentation is carried out for 15min, and the drain valve 8 is opened. When the liquid level drops to the low liquid level of the liquid level meter 7, the drain valve 8 is closed, and the drainage is completed. The water inlet pump 4-2 is started, the culture solution S2 is fed, stirring is started at the same time, when the liquid level reaches the high liquid level of the liquid level meter 7, the water inlet pump 4-2 is stopped, the anoxic phosphorus absorption process occurs, stirring is stopped after the culture is finished, sedimentation is carried out for 15min, and the water discharge valve 8 is opened. When the liquid level is reduced to the low liquid level of the liquid level meter 7, the water discharge valve 8 is closed, and the switching of the culture process is completed.
Example 1
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
Preparing a culture solution S1: COD and ammonia nitrogen concentration are respectively 200mg/L and 5.0mg/L, respectively composed of NaAc and NH 4 Cl is provided. At the same time add CaCl 2 0.03g/L，MgSO 4 0.06g/L，FeSO 4 0.0012g/L and 1.25g/L of microelement nutrition liquid. Each liter of microelement nutrition liquid contains EDTA (10 g) and FeCl 3 •6H 2 O（1.5g）、ZnSO 4 •7H 2 O（0.12g）、MnCl 2 •4H 2 O（0.12g）、H 3 BO 3 （0.15g）、KI（0.18g）、Na 2 MoO 4 •2H 2 O（0.06g）、CuSO 4 •5H 2 O（0.03g）、CoCl 2 •6H 2 O (0.15 g). The pH was about 7.2.
Preparing a culture solution S2: NO (NO) 3 - The concentration of the-N is 50mg/L, and the NaNO-N is prepared from NaNO 3 Providing. The phosphorus concentration is added according to the same concentration of phosphorus in the culture solution at the end of the phosphorus release section, KH 2 PO 4 Providing. Wherein ammonia nitrogen and CaCl 2 、MgSO 4 、FeSO 4 And the microelement nutrient solution are the same as the culture solution S1.
Taking phosphorus-containing excess sludge in a secondary sedimentation tank of a municipal sewage treatment plant, inoculating the sludge into a reactor, adding a culture solution S1 to a specified liquid level, stopping water inflow, and controlling the MLSS to be about 4000 mg/L. Through detection and calculation, the denitrifying phosphorus removal bacteria account for about 10% of the total phosphorus removal bacteria.
The anaerobic culture process is started, the concentration of dissolved oxygen is 0.05mg/L, the temperature is 31 ℃, the pH is 7.4, and the stirring speed is 55r/min.
As shown in FIG. 2, the ORP of the system gradually increases after the culture solution S1 is fed, the ORP starts to decrease as the reaction proceeds, and the rapid decrease period and the slow decrease period are presented, the ORP is rapidly decreased to-185 mV and then slowly decreased to-223 mV, and the inflection point is determined as ORP1= -185mV. And settling and draining until the culture is finished, and measuring that the concentration of P in the system is 30mg/L.
Culture solution S2 (wherein the concentration of P is 30 mg/L) is added into the reactor, and the water inlet is stopped and the anaerobic culture stage is carried out, wherein the liquid level of the culture solution is the same as that of anaerobic water inlet. The concentration of dissolved oxygen is 0.3mg/L, the temperature is 31 ℃, the pH is 7.5, and the stirring speed is 55r/min.
As shown in fig. 2, the system ORP was measured to rise to-170 mV for the first 30min, and as the reaction proceeds, the ORP began to drop, rapidly to-221 mV, and then slowly to-240 mV, with the inflection point determined as orp2= -221mV. And settling and draining until the culture is finished.
When the denitrifying phosphorus removal bacteria account for 21% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-185 mV, the reaction was stopped and the effluent was settled. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-221 mV, the reaction was stopped, the reaction was allowed to settle and drain, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic phosphorus absorption is always below 0.4mg/L, which shows that the dephosphorization effect of the sludge in the reactor is better. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 21% to 81% of all phosphorus removal bacteria, and the culture is completed, which shows that the rapid enrichment of the denitrifying phosphorus removal bacteria is realized in a short time.
Example 2
The difference from example 1 is that: COD and ammonia nitrogen concentration in the culture solution S1 are respectively 300mg/L and 10mg/L, nitrate nitrogen concentration in the culture solution S2 is 70mg/L, and P concentration is 40mg/L.
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The anaerobic phase determines the inflection point orp1= -164mV and the anoxic phase determines the inflection point orp2= -223mV. When the denitrifying phosphorus removal bacteria account for 23% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-164 mV, the reaction was stopped and the water was discharged by sedimentation. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-223 mV, the reaction was stopped, the reaction was allowed to settle and drain, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always below 0.5mg/L, which shows that the dephosphorization effect of the sludge in the reactor is better. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 23% to 87% of all phosphorus removal bacteria, and the culture is completed, which shows that the rapid enrichment of the denitrifying phosphorus removal bacteria is realized in a short time.
Example 3
The difference from example 1 is that: COD and ammonia nitrogen concentration in the culture solution S1 are respectively 100mg/L and 3mg/L, nitrate nitrogen concentration in the culture solution S2 is 50mg/L, and P concentration is 10mg/L.
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The anaerobic phase determines the inflection point orp1= -191mV and the anoxic phase determines the inflection point orp2= -229mV. When the denitrifying phosphorus removal bacteria account for 22% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-191 mV, the reaction was stopped and the water was discharged by sedimentation. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-229 mV, the reaction was stopped, the water was removed by sedimentation, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always below 0.3mg/L, which shows that the dephosphorization effect of the sludge in the reactor is better. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 22% to 77% of all phosphorus removal bacteria, and the culture is completed, which shows that the rapid enrichment of the denitrifying phosphorus removal bacteria is realized in a short time.
Example 4
The difference from example 1 is that: the carbon source is replaced with propionic acid and the ammonium chloride is replaced with ammonium nitrate.
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The anaerobic phase determines the inflection point orp1= -178mV and the anoxic phase determines the inflection point orp2= -219mV. When the denitrifying phosphorus removal bacteria account for 24% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-178 mV, the reaction was stopped and the effluent was settled. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-219 mV, the reaction was stopped, the reaction was allowed to settle and drain, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always below 0.4mg/L, which shows that the dephosphorization effect of the sludge in the reactor is better. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 24% to 75% of all phosphorus removal bacteria, and the culture is completed, which shows that the rapid enrichment of the denitrifying phosphorus removal bacteria is realized in a short time.
Example 5
The difference from example 1 is that: caCl is not added into the culture solution 2 、MgSO 4 And FeSO 4 。
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The anaerobic phase determines the inflection point orp1= -169mV and the anoxic phase determines the inflection point orp2= -227mV. When the denitrifying phosphorus removal bacteria account for 21% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-169 mV, the reaction was stopped and the water was discharged by sedimentation. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-227 mV, the reaction was stopped, and the reaction was allowed to settle and drain, and culture was performed alternately.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always more than 2.0mg/L, which shows that the dephosphorization effect of the sludge in the reactor is reduced. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 21% to 49% of all phosphorus removal bacteria. After 20 days of culture, the denitrifying phosphorus removal bacteria accounts for 70.4 percent.
Example 6
The difference from example 1 is that: the culture solution is not added with trace element nutrient solution.
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The anaerobic phase determines the inflection point orp1= -171mV and the anoxic phase determines the inflection point orp2= -220mV. When the denitrifying phosphorus removal bacteria account for 22% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-171 mV, the reaction was stopped and the water was discharged by sedimentation. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-222 mV, the reaction was stopped, the reaction was allowed to settle and drain, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always more than 1.0mg/L, which shows that the dephosphorization effect of the sludge in the reactor is reduced. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 22% to 50% of all phosphorus removal bacteria. Culturing for 20 days, and increasing the denitrification dephosphorization bacteria to 72%.
Example 7
The difference from example 1 is that: step (2) anaerobic cultivation, when ORP approaches ORP1, i.e., ORP-orp1=8mv, switching to anoxic cultivation; in the anaerobic culture, when ORP approaches ORP2, i.e., ORP-orp2=6mv, the anaerobic culture is switched.
(1) Enhanced sludge dephosphorization performance and determination of ORP inflection point in culture system
The inflection point ORP1 in the anaerobic stage and the inflection point ORP2 in the anoxic stage are the same as in example 1, and when the denitrifying phosphorus removal bacteria account for 22% of the total phosphorus removal bacteria, the sludge phosphorus removal performance strengthening process is completed.
(2) Culturing denitrifying phosphorus removal bacteria
And setting the PLC according to the ORP1 and ORP2 determined in the strengthening process, and starting to enrich denitrifying phosphorus removal bacteria. The culture solution used in the enrichment process is the same as that used in the enrichment process. Firstly, adding the culture solution S1, and stopping water inflow when the water inflow liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-177 mV, the reaction was stopped and the water was discharged by sedimentation. And switching to add the culture solution S2, and stopping water inlet when the water inlet liquid level reaches the same level as the strengthening stage liquid level. When ORP was reduced to-215 mV, the reaction was stopped, the reaction was allowed to settle and drain, and the culture was alternated.
In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always below 0.5mg/L, which shows that the dephosphorization effect of the sludge in the reactor is better. After 10 days of culture, the proportion of denitrifying phosphorus removal bacteria in the sludge is measured to be increased from 22% to 85% of all phosphorus removal bacteria, and the culture is completed.
Comparative example 1
The difference from example 1 is that: the inflection point is not adopted as a switching point in the step (2), and the same as the step (1) is adopted until sedimentation and drainage are carried out after the culture process is finished. In the cultivation process, the concentration of P in the water is measured to be more than 1.2mg/L after anoxic cultivation. Culturing for 22 days, and measuring that the proportion of denitrifying phosphorus removal bacteria in the sludge is increased from 20% to 72% of all phosphorus removal bacteria.
Comparative example 2
The difference from example 1 is that: and (3) directly performing the culture process of the step (2) after determining the inflection point without strengthening the dephosphorization performance of the sludge. In the culture process, the concentration of the effluent P after the anoxic culture in the first 3 days is higher than 10mg/L, and the concentration of the effluent P in the anoxic process from the operation to the 15 th day is always higher than 1.5mg/L, which indicates that the denitrification dephosphorization bacteria ratio in the system is less and the dephosphorization capability is weaker. From day 16, the P concentration of the effluent water after oxygen deficiency phosphorus absorption is lower than 0.5mg/L. Running to the 33 th day, the proportion of denitrifying phosphorus removal bacteria in the sludge is increased to 71 percent.
Comparative example 3
The difference from example 1 is that: the step (2) of culturing adopts the step (1) of draining water, and does not adopt fresh culture solution S2. When the ORP of the anaerobic process is reduced to ORP1 in the step (1), the concentration of the residual COD in the system is measured to be 27mg/L, and the system is supplemented with NaNO 3 Anoxic cultivation was started until the nitrate nitrogen concentration was 50 mg/L. In the culture process, the concentration of the effluent P of the anoxic section is higher than 1.5mg/L, which indicates that part of nitrate nitrogen and the residual COD in the step (1) are subjected to denitrification and denitrification process but are not used for denitrification and dephosphorization. Culturing until the denitrifying phosphorus removal bacteria in the sludge account for 73% of the total phosphorus removal bacteria, takes 16 days.
Comparative example 4
The difference from example 1 is that: the material providing COD was replaced with methanol. In the cultivation process, the concentration of P in the water measured after anoxic cultivation is always more than 1.0mg/L, which shows that the dephosphorization effect of the sludge in the reactor is not good. Culturing for 18 days, and increasing the denitrification dephosphorization bacteria to 71%.
Claims (14)
1. A rapid enrichment culture method of denitrifying phosphorus removal bacteria is characterized by comprising the following steps:
(1) And (3) strengthening the dephosphorization performance of the sludge: inoculating sludge containing denitrifying phosphorus removal bacteria into a incubator, performing anaerobic culture and determining inflection point ORP1, and draining water until the culture is finished; switching to anoxic cultivation, and determining inflection point ORP2 until drainage is finished; when the denitrifying phosphorus removal bacteria account for 20% -30% of the total phosphorus removal bacteria, the strengthening process is finished; anaerobic culture adopts culture solution S1 containing COD and ammonia nitrogen, wherein the concentration of CODcr is 100-300mg/L, and the concentration of ammonia nitrogen is 3-10mg/L; culturing by adopting a culture solution S2 containing nitrate nitrogen and phosphorus, wherein the concentration of the nitrate nitrogen is 50-70mg/L, and the total phosphorus concentration is 10-40mg/L;
(2) Enrichment culture of denitrifying phosphorus removal bacteria: taking ORP1 and ORP2 as switching points of anaerobic culture and anoxic culture, performing alternate culture, and completing enrichment culture when denitrifying phosphorus removal bacteria account for more than 70% of all phosphorus removal bacteria; stopping the reaction when the ORP is reduced to ORP-ORP1=0-10 mV, and switching to anoxic cultivation; when ORP was reduced to ORP-orp2=0-10 mV, the reaction was stopped and the anaerobic culture was switched.
2. The method according to claim 1, characterized in that: the sludge containing the denitrifying phosphorus removal bacteria in the step (1) is activated sludge containing the denitrifying phosphorus removal bacteria accounting for less than 20% of the total phosphorus removal bacteria.
3. The method according to claim 2, characterized in that: the sludge containing the denitrifying phosphorus removal bacteria is activated sludge containing 1-15% of the total phosphorus removal bacteria.
4. A method according to claim 1 or 2 or 3, characterized in that: and (3) inoculating the sludge in the step (1) according to the sludge concentration of 3000-5000mg/L after inoculation.
5. The method according to claim 1, characterized in that: the anaerobic culture conditions in the step (1) are as follows: the concentration of dissolved oxygen is lower than 0.1mg/L, the temperature is 20-35 ℃, the pH is 7.0-8.0, and the stirring speed is 50-60r/min.
6. The method according to claim 1, characterized in that: the anoxic culture conditions in the step (1) are as follows: the concentration of dissolved oxygen is 0.1-0.5mg/L, the temperature is 20-35 ℃, the pH is 7.0-8.0, and the stirring speed is 50-60r/min.
7. The method according to claim 1, characterized in that: the material for providing COD in anaerobic culture is at least one of acetic acid, sodium acetate and propionic acid, and the material for providing ammonia nitrogen is at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate and ammonium carbonate.
8. The method according to claim 1, characterized in that: the culture solution S1 also comprises CaCl 2 、MgSO 4 And FeSO 4 Wherein CaCl 2 0.02-0.03g/L MgSO 4 0.05-0.06g/L FeSO 4 0.0010-0.0012g/L.
9. The method according to claim 1 or 8, characterized in that: the culture solution S1 also comprises a trace element nutrient solution, and the concentration is 1.0-1.5g/L; wherein each liter of the microelement nutrition liquid contains 10g of EDTA and 10g of FeCl 3 •6H 2 O 1.5g、ZnSO 4 •7H 2 O 0.12g、MnCl 2 •4H 2 O 0.12g、H 3 BO 3 0.15g、KI 0.18g、Na 2 MoO 4 •2H 2 O 0.06g、CuSO 4 •5H 2 O 0.03g、CoCl 2 •6H 2 O 0.15g。
10. The method according to claim 1, characterized in that: the substance providing nitrogen by anoxic culture is nitrate and the substance providing phosphorus is orthophosphate.
11. The method according to claim 10, wherein: the substance for providing nitrogen by anoxic culture is at least one of potassium nitrate, sodium nitrate and ammonium nitrate, and the substance for providing phosphorus is at least one of potassium dihydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate and disodium hydrogen phosphate.
12. The method according to claim 1 or 10, characterized in that: the culture solution S2 also comprises ammonia nitrogen, wherein the ammonia nitrogen providing substance is at least one of ammonium sulfate, ammonium chloride, ammonium nitrate, ammonium bicarbonate and ammonium carbonate, and the concentration of the ammonia nitrogen is 3-10mg/L.
13. The method according to claim 12, wherein: the culture solution S2 also comprises a trace element nutrient solution which is the same as the trace element nutrient solution of the culture solution S1.
14. The method according to claim 1, characterized in that: and (2) finishing enrichment culture when the denitrifying phosphorus removal bacteria account for 70% -90% of the total phosphorus removal bacteria.
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Effective date of registration: 20231128 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |