CN108623015A - A kind of method of dephosphorization microculture domestication - Google Patents
A kind of method of dephosphorization microculture domestication Download PDFInfo
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- CN108623015A CN108623015A CN201810445983.8A CN201810445983A CN108623015A CN 108623015 A CN108623015 A CN 108623015A CN 201810445983 A CN201810445983 A CN 201810445983A CN 108623015 A CN108623015 A CN 108623015A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1263—Sequencing batch reactors [SBR]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/301—Aerobic and anaerobic treatment in the same reactor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2201/00—Apparatus for treatment of water, waste water or sewage
- C02F2201/002—Construction details of the apparatus
- C02F2201/007—Modular design
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention relates to sewage disposals and field of environment protection, are a kind of methods of dephosphorization microculture domestication.For under low temperature environment, denitrification dephosphorization microculture tames difficult and sewage dephosphorization effect difference problem.This is tactful using " two-part (first aerobic/anaerobic; rear anaerobic/anoxic) acclimating; from low to high water inlet loads of nutrition; water inlet, reaction, precipitation, the circular flow pattern of draining " by the present invention, enhances the enriched amount of dephosphorization microorganism, improves microbial reproduction rate, creates optimal condition for microbial reproduction.The present invention is run by the aerobic/anaerobic of 40d, the anaerobic/anoxic operation of 30d, successfully acclimating to denitrification dephosphorization microorganism, and phosphorus concentration is stablized in 0.5mg/L in water outlet.
Description
Technical field
The present invention relates to sewage disposal and field of environment protection, specifically a kind of side of dephosphorization microculture domestication
Method.
Background technology
Nitrogen and phosphorus element is to cause two important factors of body eutrophication, and numerous countries suffer from its concentration of emission
Stringent limitation.The direct result of body eutrophication is exactly to accelerate water body ager process, and water body is made to lose original fishery, tourism
The function of industry and drinking water source.National environmental protection portion of China requires urban wastewater treatment firm water outlet to be discharged into major river valley and lake
When the closing such as pool, reservoir, semiclosed waters, execute《Urban wastewater treatment firm pollutant emission standard》(GB18918-2002)
Level-one A standards.With nitrogen, the raising of phosphorus discharge standard, during newly-built sewage treatment plant or old technology upgrading, efficiently
The Biological Nitrogen Removal Processe of rate low energy consumption is using particularly important.
Currently, Enhanced Biological Phosphorus Removal technique (enhanced biological phosphorus removal, EBPR) because
Its is efficient, at low cost and environmental-friendly and receives focus of attention.Main functional microorganism is polyP bacteria in the technique
(Accumulibacter), it is mainly characterized by:Cell cohesion phosphorus hydrolysis under anaerobic generates energy (adenosine
Triphosphate, ATP) and reduced coenzyme NADH, for volatile fatty acid (volatile fatty acid, VFA)
It absorbs and is stored as poly-β-hydroxybutyric acid (poly- β-hydroxybutyrate, PHB);Then it is electricity with PHB under aerobic condition
Sub- donor carries out excessive suction phosphorus by electron acceptor of oxygen, to remove the phosphorus in sewage in the form of excess sludge discharge.With
Further investigation of the scholar to polyP bacteria, find there is a kind of polyP bacterias can be under anoxic conditions using nitrate as electron acceptor
Synchronize denitrification nitrogen and phosphorus removal, referred to as denitrification synchronous denitrification dephosphorizing bacterium.Denitrification synchronous denitrification dephosphorizing bacterium is compared to good
Oxygen polyP bacteria can save aeration quantity 30%, reduce sludge yield 50%, reduce carbon source demand amount 50% and be particularly suitable for low C/N ratios
The processing of sanitary sewage.Either aerobic phosphorous accumulating bacterium or denitrification synchronous denitrification dephosphorizing bacterium is all highly prone in enrichment process
Do not have with similar metabolic type but the fusca xylanase competition released phosphorus and inhale phosphorus function, and the factors such as pH, DO, carbon source and temperature will
Affect the enrichment environment of polyP bacteria and fusca xylanase.In these influence factors, temperature is the master of polyP bacteria and fusca xylanase enrichment
Want influence factor.Domestic and foreign scholars make the acclimating of denitrification dephosphorization microorganism in room temperature (25 ± 5 DEG C)
Certain research achieves some achievements in research and successful experience.
However, research dephosphorization microculture acclimation method, being applied to practice to EBPR systems has important guidance to anticipate
Adopted (especially winter).
Invention content
The purpose of the present invention is to provide a kind of methods of dephosphorization microculture domestication.
The object of the present invention is achieved like this:
(1) fill phase:Solution in inlet tank 1 is pumped into reactor 12 using intake pump 2, by being controlled into water controller 5
Flooding time processed is 30min;
(2) anaerobic stages:Start blast aeration machine 3 to blast nitrogen in reactor 12 by aeration head 11, anaerobism ring is provided
Border, be passed through the nitrogen time by aeration controller 6 control be 2h;Starting machine mixer 7 drives agitating paddle 10 to be stirred, and provides
The power that solution is thoroughly mixed, mechanical agitation time are 2h by the control of mechanical agitation controller 8;
(3) aerobic stage:After the completion of anaerobic reaction 2h, reactor anaerobic environment is changed to aerobic environment, by blast aeration
Machine 3 is blasted air in reactor 12 by aeration head 11, provides aerobic environment, is passed through air time and is controlled by aeration controller 6
For 4h;Starting machine mixer 7 drives agitating paddle 10 to be stirred, and provides the power that solution is thoroughly mixed, mechanical agitation time
It is 4h by the control of mechanical agitation controller 8;
(4) precipitate phase:After the completion of aerobic reaction 4h, blast aeration machine 3, mechanical agitation is automatically closed in aeration controller 6
Machine mixer 7, sedimentation time 1h is automatically closed in controller 8;
(5) bleeding stage:After the completion of precipitating 1h, start draining pump 14 and be discharged into supernatant is precipitated in water tank 15, drains
Time is 30min by the control of water discharge controller 9;
(6) step (1), step (2), step (3), step (4), step (5) are repeated, to complete water inlet, anaerobism, good again
Oxygen, precipitation and drainage procedure, this 120 period of process operation amount to 40d;
(7) after the completion of aerobic/anaerobic stage 40d, the operating status of reactor is changed to the anaerobic/anoxic stage;
(8) fill phase:It is identical with step (1), but influent load reduces;
(9) anaerobic stages:It is identical with step (2);
(10) anoxic phases:After the completion of anaerobic reaction 2h, reactor anaerobic environment is changed to by the way that nitrate solution is added
Anaerobic environment starts machine mixer 7 and agitating paddle 10 is driven to be stirred, provides the power that solution is thoroughly mixed, mechanical agitation
Time is 4h by the control of mechanical agitation controller 8;
(11) precipitate phase:Same step (4);
(12) bleeding stage:Same step (5)
(13) step (8), step (9), step (10), step (11), step (12) are repeated, to complete to intake again, detest
Oxygen, anoxic, precipitation and drainage procedure, this 90 period of process operation amount to 30d;
(14) taken at regular intervals is discharged water sample, measures PO in water outlet4 3-- P concentration;
Solution C OD a concentration of 800mg/L, PO in step (1) described inlet tank4 3-A concentration of 40mg/L of-P.
The rate that step (2) the blast aeration machine is passed through nitrogen is 1.5L/min.
The rate that step (3) the blast aeration machine is passed through air is 1.5L/min.
Solution C OD a concentration of 300mg/L, PO in step (8) described inlet tank4 3-A concentration of 20mg/L of-P.
Nitrate concentration described in step (10) is 30mg/L.
PO described in step (14)4 3-- P method for measurement of concentration is molybdenum-antimony anti-spectrophotometric method.
The method is characterized in that:
(1) two-part culture is used to tame dephosphorization microorganism, first the aerobic dephosphorization of acclimating is micro- under the conditions of aerobic/anaerobic
Biology;The dephosphorization microorganism under anaerobic environment is cultivated under the conditions of anaerobic/anoxic again.
(2) using water inlet, reaction, precipitation, the circular flow pattern of draining, influence of the nitrate to anaerobic phosphorus release is reduced,
And reduce influence of the carbon source to denitrification nitrogen and phosphorus removal.
(3) from low to high water inlet loads of nutrition is used, loads of nutrition is high in initial operating stage water inlet, accelerates dephosphorization microorganism
Fast enriching at low ambient temperatures;The phase reduces loads of nutrition in water inlet after operation, keeps the denitrification dephosphorization of acclimating micro-
The normal sanitary sewage of biological adaptation.
The present invention specific implementation measure be:
(1) it the design of reactor and builds:Reactor design use sequencing batch active sludge, mainly by water inlet system,
Aeration agitation system, outlet system and time controlled system composition.The present apparatus can by each controller from time series into
Water, reaction, precipitation, drainage procedure are controlled, and are realized automatic operating management, are significantly reduced artificial intensity, save dirt
Treatment cost is contaminated, and device structure is simple, is moved easily.
(2) aerobic/anaerobic operational mode:Sewage treatment plant's aerobic tank sludge seeding is anti-to the sequencing batch active sludge
It answers in device, the acclimating dephosphorization microorganism under aerobic/anaerobic environment, COD a concentration of 800mg/L, PO in control water inlet4 3--P
A concentration of 40mg/L, its PO of daily acquisition water outlet water determination4 3-- P concentration, with PO in water outlet4 3-- P concentration is stable and reaches row
Standard is put to determine that acclimating work is completed.
(3) anaerobic/anoxic operational mode:The acclimating denitrification dephosphorization microorganism under anaerobic/anoxic environment, control into
A concentration of 300mg/L of COD, PO in water4 3-A concentration of 20mg/L of-P, its PO of daily acquisition water outlet water determination4 3-- P concentration, to go out
PO in water4 3-- P concentration is stable and reaches discharge standard to determine that acclimating work is completed.
The present invention is suitable for the quick startup of Enhanced Biological Phosphorus Removal technique, has very strong practical significance and is widely applied
Property.
For the present invention compared with domestic and international existing similar technique, creativeness is the following:
1, the culture domestication for having studied denitrification dephosphorization microorganism in Enhanced Biological Phosphorus Removal technique, is winter most area
Sewage disposal qualified discharge provides possibility;
2, using from low to high water inlet loads of nutrition, the acclimating rate of microorganism is accelerated;
3, using water inlet, reaction, precipitation, the circular flow pattern of draining, optimal condition is created for microbial reproduction.
The difference of the present invention and the domestic and international prior art are:
1, using two-part (first aerobic/anaerobic, rear anaerobic/anoxic) acclimating method, the richness of dephosphorization microorganism is enhanced
Collect quantity;
2, using from low to high water inlet loads of nutrition, microbial reproduction rate is improved;
3, using water inlet, reaction, precipitation, the circular flow pattern of draining, optimal condition is created for microbial reproduction.
The beneficial effects of the invention are as follows:
Method for domesticating is cultivated using two-part (first aerobic/anaerobic, rear anaerobic/anoxic), enhances the enrichment of dephosphorization microorganism
Quantity;Using from low to high water inlet loads of nutrition, microbial reproduction rate is improved;It is followed using water inlet, reaction, precipitation, draining
Inscription of loop pattern creates optimal condition for microbial reproduction.By this strategy, Enhanced Biological Phosphorus Removal technique can be improved low
The operational effect of warm condition.
Description of the drawings
Fig. 1 is the reactor schematic diagram of the present invention.
Figure label is as follows:1, intake pool;2, intake pump;3, aerator;4, water inlet;5, water inlet pump controller;6, it exposes
Mechanism of qi controller;7, blender;8, mechanical agitation machine controller;9, water outlet pump;10, agitating paddle;11, aeration head;12, SBR is anti-
Answer device;13, water outlet;14, water outlet pump;15, discharge bay.
Specific implementation mode
Example 1
It is as follows to test influent quality:
1~40d, COD=800mg/L, PO4 3-- P=40mg/L;
41~70d, COD=300mg/L, PO4 3-- P=20mg/L;NO3 -- N=30mg/L
Environment temperature is 5~10 DEG C when test run, and anaerobic stages control dissolved oxygen concentration and are less than 0.1mg/L;Aerobic rank
Section control dissolved oxygen concentration is more than 2.0mg/L;It is -100~-40mV that anoxic phases, which control oxidation-reduction potential,.
Specific processing is as follows:
(1) sewage treatment plant's aerobic tank sludge is taken to make sequencing batch active sludge reactor seed sludge, sludge concentration is
4200mg/L。
(2) high-load anaerobic/aerobic stage (1~40d):Daily operation 3 periods, each period according to water inlet 30min,
Automatic running goes out by the aerobic/anaerobic operation of 40d successively by anaerobic reaction 2h, aerobic reaction 4h, precipitation 1h, draining 30min
Phosphorus concentration is stablized in 0.5mg/L hereinafter, tp removal rate reaches 98.8% in water.
(3) underload anaerobic/anoxic stage (41~70d):Daily 3 periods of operation, each period is according to water inlet
30min, anaerobic reaction 2h, hypoxia response 4h, precipitation 1h, 30min automatic runnings successively are drained, is transported by the anaerobic/anoxic of 30d
Row, phosphorus concentration is stablized in 0.5mg/L hereinafter, tp removal rate reaches 98.5% in water outlet.Thus illustrate, by the way that " two-part (is first detested
Oxygen/aerobic, rear anaerobic/anoxic) acclimating, from low to high water inlet loads of nutrition, water inlet, reaction, precipitation, the circulating drained
This strategy of row pattern " can effectively be enriched to denitrification dephosphorization microorganism.
Example 2
Using actual domestic wastewater as water inlet, influent quality:COD is 196mg/L, PO4 3-- P=3.8mg/L, by training
The processing of dephosphorization microorganism after domesticating, water outlet phosphorus concentration are stablized in 0.5mg/L hereinafter, tp removal rate reaches 99.2%.
Above-described embodiment is only preferred examples of the present invention, and is not intended to limit the present invention, all within the principle of the present invention,
Any equivalent substitute, the modifications and variations done, within protection scope of the present invention.
Claims (2)
1. a kind of method of dephosphorization microculture domestication, the described method comprises the following steps:
(1) it the design of reactor and builds:Reactor design uses sequencing batch active sludge, mainly by water inlet system, aeration
Stirring system, outlet system and time controlled system composition.The present apparatus can by each controller from time series to water inlet, anti-
Answer, precipitate, drainage procedure is controlled, realize automatic operating management, significantly reduce artificial intensity, save pollution control
Cost, and device structure is simple, is moved easily.
(2) aerobic/anaerobic operational mode:By sewage treatment plant's aerobic tank sludge seeding to the sequencing batch active sludge reactor
In, the acclimating dephosphorization microorganism under aerobic/anaerobic environment, COD a concentration of 800mg/L, PO in control water inlet4 3-- P concentration
For 40mg/L, its PO of daily acquisition water outlet water determination4 3-- P concentration, with PO in water outlet4 3-- P concentration is stable and reaches discharge mark
Standard is completed to determine that acclimating works.
(3) anaerobic/anoxic operational mode:The acclimating denitrification dephosphorization microorganism under anaerobic/anoxic environment, in control water inlet
COD a concentration of 300mg/L, PO4 3-A concentration of 20mg/L of-P, its PO of daily acquisition water outlet water determination4 3-- P concentration, in water outlet
PO4 3-- P concentration is stable and reaches discharge standard to determine that acclimating work is completed.
2. a kind of denitrification dephosphorization microculture acclimation method according to claim 1, it is characterised in that:In step (1)
Flooding time is 30min, reaction time (anaerobism 2h, aerobic or anoxic 4h), sedimentation time 1h, water discharge time 30min.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111440725A (en) * | 2020-04-17 | 2020-07-24 | 广东省微生物研究所(广东省微生物分析检测中心) | Method for undisturbed enrichment and domestication of functional microorganisms in water sediments |
CN114426334A (en) * | 2020-10-10 | 2022-05-03 | 中国石油化工股份有限公司 | Rapid enrichment culture method of denitrifying phosphorus removal bacteria |
-
2018
- 2018-05-11 CN CN201810445983.8A patent/CN108623015A/en not_active Withdrawn
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111440725A (en) * | 2020-04-17 | 2020-07-24 | 广东省微生物研究所(广东省微生物分析检测中心) | Method for undisturbed enrichment and domestication of functional microorganisms in water sediments |
CN111440725B (en) * | 2020-04-17 | 2024-03-01 | 广东省微生物研究所(广东省微生物分析检测中心) | Method for enriching and domesticating functional microorganisms in undisturbed state of water sediment |
CN114426334A (en) * | 2020-10-10 | 2022-05-03 | 中国石油化工股份有限公司 | Rapid enrichment culture method of denitrifying phosphorus removal bacteria |
CN114426334B (en) * | 2020-10-10 | 2023-05-05 | 中国石油化工股份有限公司 | Rapid enrichment culture method of denitrifying phosphorus removal bacteria |
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Application publication date: 20181009 |