CN105543158B

CN105543158B - Nitrobacteria growth promoter and preparation method and application thereof

Info

Publication number: CN105543158B
Application number: CN201410585482.1A
Authority: CN
Inventors: 孙丹凤; 高会杰; 郭志华; 赵胜楠; 许谦
Original assignee: China Petroleum and Chemical Corp; Sinopec Dalian Research Institute of Petroleum and Petrochemicals
Current assignee: Sinopec Dalian Petrochemical Research Institute Co ltd; China Petroleum and Chemical Corp
Priority date: 2014-10-28
Filing date: 2014-10-28
Publication date: 2020-02-14
Anticipated expiration: 2034-10-28
Also published as: CN105543158A

Abstract

The invention discloses a nitrobacteria growth promoter, which comprises 40-100 parts by weight of metal salt and preferably 50-80 parts by weight of polyamine substance, and 5-30 parts by weight of polyamine substance and preferably 10-20 parts by weight of metal salt; the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (5-25): (1-8): 0.5-5), preferably (8-12): 10-20): 2-6): 1-4. The accelerant has simple formula and easy preparation, can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system, so that the nitrobacteria can rapidly grow and reproduce in a short time, and the cultured nitrobacteria has high activity and strong impact resistance.

Description

Nitrobacteria growth promoter and preparation method and application thereof

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a nitrobacteria growth promoter, and a preparation method and application thereof.

Background

In the biological denitrification method, ammonia nitrogen is removed by nitrification of nitrifying bacteria in both traditional nitrification-denitrification, novel shortcut nitrification-denitrification and novel shortcut nitrification-anaerobic ammonia oxidation. Nitrifying bacteria belong to the group of chemolithotrophic microorganisms. Biological cells can utilize only energy stored in the form of ATP or the like, and cannot directly utilize free energy released by chemical reaction. In aerobic metabolism, ATP is synthesized mainly by oxidative phosphorylation of the respiratory chain. The ammoxidation phosphorylation efficiency is very low, the ATP generated by the ammoxidation phosphorylation is very limited, and the energy is mainly used for electron transition to a higher energy level, so that the nitrobacteria grow very slowly, and the generation period is 8-36 h. The content of peptidoglycan in the cell wall of nitrobacteria is low, and the content of protein and fat is high, so that the nitrobacteria is sensitive to environmental change, the adaptability and tolerance of natural nitrobacteria in nature are poor, and the nitrobacteria cannot compete with heterotrophic microorganisms in growth under a plurality of conditions to obtain advantages. In a sewage treatment system, when the content of nitrobacteria in activated sludge is low, the nitrobacteria cannot grow and propagate quickly in a short time by adjusting the environmental conditions such as dissolved oxygen, pH and the like, and finally the ammonia nitrogen removal capability of the existing running sewage treatment system is limited. Whether the nitrifying bacteria are cultured in the sewage treatment system directly or outside the sewage treatment system, substances for promoting growth need to be added to accelerate the growth rate of the nitrifying bacteria.

On a biochemical level, the nitration reaction is a metabolic pathway involving the co-catalysis of ammonia monooxygenase, hydroxylamine oxidoreductase and nitrite oxidase, with a complex conversion of substances and energy. The biological promoter is used for improving the activity of the enzymes in the nitrifying bacteria, so that the problem of slow growth of the nitrifying bacteria is solved, and the nitration reaction process can be accelerated. At present, many researches on biological promoters are carried out, and CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X respectively propose nitrobacteria growth promoters formed by combining different metal salts, wherein the main components comprise molasses, metal salts (ferrous salt, manganese salt, calcium salt and magnesium salt) and an adsorbent. The ammonia nitrogen removal rate can be improved by more than 20 percent after the accelerator is used. However, the addition of the adsorbents mainly comprises substances such as zeolite powder, diatomite, powdered activated carbon or fly ash and the like, which inevitably increases the sludge yield. CN201110315549.6 discloses a method for culturing shortcut nitrification and denitrification granular sludge, which is characterized in that 5-15 mg/L hydroxylamine is added periodically; CN201010168453.7 discloses a method for rapidly starting an anoxic and ammoxidation biofilter, which is characterized in that hydroxylamine is added in the step (2) to induce the inoculated sludge to be converted into an anoxic and ammoxidation biomembrane. CN201410141638.7 discloses a culture solution of nitrosobacteria and a preparation and culture method thereof, wherein the formula of the culture solution is relatively complex, and the culture solution not only relates to elements for providing cell growth such as iron, calcium, potassium, magnesium and the like, but also relates to elements for promoting enzyme synthesis such as cobalt, molybdenum, manganese, zinc and the like.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a nitrobacteria growth promoter and a preparation method and application thereof. The accelerant has simple formula and easy preparation, can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system, so that the nitrobacteria can rapidly grow and reproduce in a short time, and the cultured nitrobacteria has high activity and strong impact resistance.

The nitrobacteria growth promoter comprises 40-100 parts by weight of metal salt and preferably 50-80 parts by weight of polyamine substance, and 5-30 parts by weight of polyamine substance and preferably 10-20 parts by weight of metal salt and polyamine substance; the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (5-25): (1-8): 0.5-5), preferably (8-12): 10-20): 2-6): 1-4.

The calcium salt is CaSO₄Or CaCl₂Preferably CaSO₄(ii) a The magnesium salt is MgSO₄Or MgCl₂Preferably MgSO (MgSO)₄(ii) a The ferrous salt is FeSO₄Or FeCl₂Preferably FeSO₄(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuSO₄。

The polyamine substance of the invention is spermine, spermidine or a mixture of the spermine and the spermidine.

The accelerator provided by the invention also can comprise inorganic hydroxylamine acid, and the content is 0.5-15 parts by weight, preferably 2-10 parts by weight. The inorganic acid hydroxylamine is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate, and is preferably hydroxylamine sulfate.

The preparation method of the nitrobacteria growth promoter comprises the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: the metal salt is 40-100 parts by weight, preferably 50-80 parts by weight, the metal salt is calcium salt, ferrous salt and copper salt, and Ca in the metal salt²⁺、Mg²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (5-25): (1-8): 1-4), preferably (8-12): 10-20): 2-6): 1-4; (2) before use, 5-30 parts by weight, preferably 10-20 parts by weight of polyamine substances are added into the metal salt solution.

The calcium salt in the step (1) of the invention is CaSO₄Or CaCl₂Preferably CaSO₄(ii) a Magnesium salt is Mg SO₄Or MgCl₂Preferably MgSO (MgSO)₄(ii) a The ferrous salt is FeSO₄Or FeCl₂Preferably FeSO₄(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuSO₄。

The polyamine substance in the step (2) of the invention is spermine, spermidine or a mixture of the spermine and the spermidine.

Further, 0.5-15 parts by weight, preferably 2-10 parts by weight of inorganic acid hydroxylamine is added before use in step (2) of the invention, wherein the inorganic acid hydroxylamine is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate, preferably hydroxylamine sulfate.

The nitrobacteria growth promoter provided by the invention can be used in the culture process of nitrobacteria, can be used for improving the ammonia nitrogen removal effect of a sewage treatment system, and can also be used for quickly starting an ammonia-containing system and quickly recovering an impacted system. The dosage is determined according to the sewage property, the treatment capacity and the treatment effect. In the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 10-100 mg/L in the sewage.

Compared with the prior art, the invention has the following beneficial effects:

1. the metal ions provided by the metal salt are used as elements required by the growth of nitrobacteria, and simultaneously used as components of enzyme to improve the activity of the enzyme, so that the substrate can be rapidly degraded, and the enzymatic reaction process is accelerated. The polyamine substance and the metal ions act together to accelerate cell proliferation, improve the settleability and stability of the harvested thalli and prolong the service life of the thalli.

2. The appropriate amount of the inorganic hydroxylamine acid can be used as a matrix of hydroxylamine oxidoreductase to directly participate in the metabolic process of nitrobacteria, shorten the enzymatic reaction process, and simultaneously can be used as an activator of cells to accelerate the growth of the cells.

3. The invention realizes the purpose of quickly culturing nitrobacteria under the combined action of metal salt, polyamine substances and inorganic hydroxylamine acid by selecting the components and the proportion of the nitrobacteria growth promoter. The invention solves the problem of slow growth of nitrifying bacteria, and can accelerate the nitration reaction process and improve the treatment effect of waste water. The growth rate of nitrobacteria can be increased by 10-100 times after the accelerator is used, the ammonia nitrogen removal rate of ammonia-containing wastewater can be improved by more than 30%, and the problem of quick recovery of the nitrification deamination nitrogen capacity of an impacted sewage treatment system can be well solved.

Detailed Description

The present invention will be described in detail with reference to specific examples.

The formulations of the nitrifying bacteria growth promoting agents of examples 1 to 8 and comparative examples 1 to 4 are shown in Table 1.

TABLE 1 formulation and proportions of accelerators

The COD concentration of main pollutants contained in the wastewater discharged by a certain enterprise is about 800mg/L, the ammonia nitrogen concentration is about 200mg/L, the factory adopts a batch activated sludge process (SBR process) for treatment, and the COD concentration in the treated effluent is less than 60mg/L, but the ammonia nitrogen concentration is still higher than 60 mg/L. The concentration of the accelerator prepared according to the proportion in the table is 0.5g/L, the accelerator is added according to the concentration of 20mg/L in the sewage every day, after the accelerator is added for 15 days, the ammonia nitrogen removal rate is improved by more than 10%, the adding is stopped, the system continues to run for 10 days, and a water sample is taken to analyze the concentration of suspended matters in the effluent and the concentration of ammonia nitrogen in the effluent and calculate the ammonia nitrogen removal rate. The specific treatment results after addition are shown in Table 2.

TABLE 2 treatment Effect with different formulations of accelerators

From the quality of the effluent water in each embodiment of table 2, under the condition that no accelerator is added, the concentration of effluent suspended matters is 150mg/L, the concentration of effluent ammonia nitrogen is 80mg/L, the removal rate of ammonia nitrogen is only 60%, the concentration of the effluent suspended matters after the accelerator is added is lower than 100mg/L, the concentration of the effluent ammonia nitrogen is lower than 15mg/L, the removal rate of ammonia nitrogen is higher than 90%, when the accelerator only contains metal salts or polyamine substances in the formula, the concentration of the effluent suspended matters is between 120 and 140mg/L, the concentration of the effluent ammonia nitrogen is still between 50 and 70mg/L, and the removal rate of ammonia nitrogen is lower than 80%. Therefore, the growth promoter can obviously improve the removal rate of ammonia nitrogen in sewage. In addition, the flocculation performance of the sludge can be improved, and the concentration of effluent suspended matters is reduced.

Claims

1. A nitrobacteria growth promoter is characterized by comprising 50-70 parts by weight of metal salt and 10-18 parts by weight of polyamine substance; the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, Ca²⁺、Mg²⁺、Fe²⁺And Cu²⁺The molar ratio of (8-12): (10-20): (2-6): 1-4); the polyamine substance is spermine, spermidine or a mixture of the spermine and the spermidine.

2. The growth promoter according to claim 1, characterized in that: the calcium salt is CaSO₄Or CaCl₂Magnesium salt is MgSO₄Or MgCl₂The ferrous salt is FeSO₄Or FeCl₂The copper salt is CuSO₄Or CuCl₂。

3. The growth promoter according to claim 1, characterized in that: the calcium salt is CaSO₄Magnesium salt is MgSO₄The ferrous salt is FeSO₄The copper salt is CuSO₄。

4. The growth promoter according to claim 1, characterized in that: the accelerator also comprises 0.5-15 parts by weight of inorganic hydroxylamine acid.

5. A growth promoter according to claim 4, characterized in that: the inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate, and the content is 2-10 parts by weight.

6. A growth promoter according to claim 5, characterized in that: the inorganic hydroxylamine acid is hydroxylamine sulfate.

7. A preparation method of a nitrobacteria growth promoter is characterized by comprising the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: 50-70 parts by weight of metal salt, wherein the metal salt is calcium salt, magnesium salt, ferrous salt and copper salt, and Ca in the metal salt²⁺、Mg²⁺、Fe²⁺And Cu²⁺The molar ratio of (8-12): (10-20): (2-6): 1-4); (2) before use, 10-18 parts by weight of polyamine substances are added into a metal salt solution.

8. The method of claim 7, wherein: and (3) adding 0.5-15 parts by weight of inorganic hydroxylamine acid before use in the step (2), wherein the inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate.

9. The method of claim 8, wherein: adding 2-10 parts by weight of inorganic hydroxylamine acid.

10. Use of a growth promoter according to any one of claims 1 to 6, characterized in that: in the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 10-100 mg/L in the sewage.