CN105624089B

CN105624089B - Promoter for culturing ammonia oxidizing bacteria and preparation method thereof

Info

Publication number: CN105624089B
Application number: CN201410585422.XA
Authority: CN
Inventors: 高会杰; 孙丹凤; 郭志华; 赵胜楠; 郭宏山
Original assignee: China Petroleum and Chemical Corp; Sinopec Dalian Research Institute of Petroleum and Petrochemicals
Current assignee: Sinopec Dalian Petrochemical Research Institute Co ltd; China Petroleum and Chemical Corp
Priority date: 2014-10-28
Filing date: 2014-10-28
Publication date: 2020-07-07
Anticipated expiration: 2034-10-28
Also published as: CN105624089A

Abstract

The invention discloses an accelerant for culturing ammonia oxidizing bacteria, which comprises metal salt, polyamine substances, inorganic hydroxylamine acid and Na₂SO₃The metal salt is 40 to 100 parts by weight, preferably 50 to 80 parts by weight, the polyamine substance is 5 to 30 parts by weight, preferably 10 to 20 parts by weight, the inorganic hydroxylamine acid is 0.05 to 1.5 parts by weight, preferably 0.1 to 1.0 part by weight, and Na₂SO₃10 to 40 parts by weight, preferably 20 to 30 parts by weight; the metal salt is calcium salt, magnesium salt and copper salt, Ca²⁺、Mg²⁺And Cu²⁺The molar ratio of (5-15): (5-25): (0.5-5), preferably (8-12): 10-20): 1-4. The accelerant has simple formula and easy preparation, can be used in the culture process of ammonia oxidizing bacteria, and can also be directly added into a sewage treatment system to accelerate the start of the shortcut nitrification-denitrification and shortcut nitrification-anaerobic ammonia oxidation processes and realize stable operation.

Description

Promoter for culturing ammonia oxidizing bacteria and preparation method thereof

Technical Field

The invention belongs to the technical field of biology, and particularly relates to an accelerant for culturing ammonia oxidizing bacteria and a preparation method thereof.

Background

Biological denitrification is mainly accomplished by nitrifying and denitrifying bacteria. Nitrifying bacteria belong to the group of chemolithotrophic microorganisms. Biological cells can utilize only energy stored in the form of ATP or the like, and cannot directly utilize free energy released by chemical reaction. In aerobic metabolism, ATP is synthesized mainly by oxidative phosphorylation of the respiratory chain. The ammoxidation phosphorylation efficiency is very low, the ATP generated by the ammoxidation phosphorylation is very limited, and the energy is mainly used for electron transition to a higher energy level, so that the nitrobacteria grow very slowly, and the generation period is 8-36 h. The content of peptidoglycan in the cell wall of nitrobacteria is low, and the content of protein and fat is high, so that the nitrobacteria is sensitive to environmental change, the adaptability and tolerance of natural nitrobacteria in nature are poor, and the nitrobacteria cannot compete with heterotrophic microorganisms in growth under a plurality of conditions to obtain advantages. In a sewage treatment system, when the content of nitrobacteria in activated sludge is low, the nitrobacteria cannot grow and propagate quickly in a short time by adjusting the environmental conditions such as dissolved oxygen, pH and the like, and finally the ammonia nitrogen removal capability of the existing running sewage treatment system is limited.

Nitrifying bacteria are further classified into ammonia oxidizing bacteria (nitrite bacteria) and nitrite oxidizing bacteria (nitrate bacteria), and novel biological denitrification technologies developed in recent years, such as shortcut nitrification-denitrification and shortcut nitrification-anaerobic ammonia oxidation, require that nitrification reaction is terminated by proceeding to a nitrite stage, and it is desirable that denitrification is performed directly after ammonia nitrogen is converted into nitrite nitrogen by ammonia oxidizing bacteria without further oxidation. Therefore, enrichment culture of ammonia oxidizing bacteria is very critical. On a biochemical level, the nitration reaction is a metabolic pathway involving the co-catalysis of ammonia monooxygenase, hydroxylamine oxidoreductase and nitrite oxidase, with a complex conversion of substances and energy. The biological promoter is adopted to improve the activities of ammonia monooxygenase and hydroxylamine oxidoreductase in nitrifying bacteria, inhibit the activity of nitrite oxidase, control the nitration reaction process and is one of effective ways for solving the problem that the nitration reaction is stopped when going to the nitrite stage.

At present, many researches on biological promoters are carried out, and CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X respectively propose nitrobacteria growth promoters formed by combining different metal salts, wherein the main components comprise molasses, metal salts (ferrous salt, manganese salt, calcium salt and magnesium salt) and an adsorbent. The ammonia nitrogen removal rate can be improved by more than 20 percent after the accelerator is used. However, the addition of the adsorbents mainly comprises substances such as zeolite powder, diatomite, powdered activated carbon or fly ash and the like, which inevitably increases the sludge yield. CN201110315549.6 discloses a method for culturing shortcut nitrification and denitrification granular sludge, which is characterized in that 5-15 mg/L hydroxylamine is added periodically; CN201010168453.7 discloses a method for rapidly starting an anoxic and ammoxidation biofilter, which is characterized in that hydroxylamine is added in the step (2) to induce the inoculated sludge to be converted into an anoxic and ammoxidation biofilm, the method mainly utilizes the inhibition effect of the hydroxylamine on nitrite oxidizing bacteria to promote the shortcut nitrification, but does not promote the self growth of slow-growing ammoxidation bacteria. CN201410141638.7 discloses a culture solution of nitrosobacteria and a preparation and culture method thereof, wherein the formula of the culture solution is relatively complex, and the culture solution not only relates to elements for providing cell growth such as iron, calcium, potassium, magnesium and the like, but also relates to elements for promoting enzyme synthesis such as cobalt, molybdenum, manganese, zinc and the like.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a growth promoter for culturing ammonia oxidizing bacteria and a preparation method thereof. The accelerant has simple formula and easy preparation, can be used in the culture process of ammonia oxidizing bacteria, and can also be directly added into a sewage treatment system to accelerate the start of the shortcut nitrification-denitrification and shortcut nitrification-anaerobic ammonia oxidation processes and realize stable operation.

The promoter for culturing ammonia oxidizing bacteria comprises metal salt, polyamine substances, inorganic acid hydroxylamine and Na₂SO₃The metal salt accounts for 40-100 parts by weight, preferably 50-80 parts by weight, and the polyamine substance accounts for 5-30 parts by weight, preferably 10-20 parts by weight; 0.05 to 1.5 parts by weight of hydroxylamine inorganic acid, preferably 0.1 to 1.0 part by weight of Na₂SO₃10 to 40 parts by weight, preferably 20 to 30 parts by weight; the metal salt is calcium salt, magnesium salt and copper salt, Ca²⁺、Mg²⁺And Cu²⁺The molar ratio of (5-15): (5-25): (0.5-5), preferably (8-12): 10-20): 1-4.

The calcium salt is CaSO₄Alternatively, CaCl is preferred₂(ii) a The magnesium salt is MgSO₄Or MgCl₂Preferably MgCl₂(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuCl₂。

The polyamine substance of the invention is spermine, spermidine or a mixture of the spermine and the spermidine.

The inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate, and is preferably hydroxylamine hydrochloride.

The preparation method of the accelerant for culturing ammonia oxidizing bacteria comprises the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: the metal salt is 40-100 parts by weight, preferably 50-80 parts by weight, the metal salt is calcium salt, magnesium salt and copper salt, and Ca in the metal salt²⁺、Mg²⁺And Cu²⁺The molar ratio of (5-15): (5-25): 0.5-5), preferably (8-12): 10-20): 1-4; (2) 10 to 40 parts by weight, preferably 20 to 30 parts by weight of Na₂SO₃Adding into metal salt solution; (3) before use, 5 to 30 parts by weight, preferably 10 to 20 parts by weight of polyamine substance and 0.05 to 1.5 parts by weight, preferably 0.1 to 1.0 part by weight of inorganic hydroxylamine acid are added into the metal salt solution.

The calcium salt in the step (1) of the invention is CaSO₄Or CaCl₂Preferably CaCl₂(ii) a The magnesium salt is MgSO₄Or MgCl₂Preferably MgCl₂(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuCl₂。

The polyamine substance in the step (3) of the invention is spermine, spermidine or a mixture of the spermine and the spermidine. The inorganic acid hydroxylamine is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate, and is preferably hydroxylamine hydrochloride.

The culture ammonia oxidizing bacteria accelerant can be used in the culture process of ammonia oxidizing bacteria, can be used for improving the ammonia nitrogen removal effect of a sewage treatment system, and can also be used for the quick start of a shortcut nitrification-denitrification system and a shortcut nitrification-anaerobic ammonia oxidation system and the quick recovery of an impacted system. The dosage is determined according to the sewage property, the treatment capacity and the treatment effect. In the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 0.5-50 mg/L in the sewage.

Compared with the prior art, the invention has the following beneficial effects:

1. the metal ions provided by the metal salt are used as elements required by the growth of ammonia oxidizing bacteria, and simultaneously used as enzyme components to improve the activity of the enzyme, so that the substrate can be rapidly degraded, and the enzymatic reaction process is accelerated. The polyamine substance and the metal ions act together to accelerate cell proliferation, improve the settleability and stability of the harvested thalli and prolong the service life of the thalli.

2. Inorganic acid hydroxylamine and Na₂SO₃The addition of the hydroxylamine complex is helpful for controlling the dissolved oxygen of the system, so that the hydroxylamine can be used as a substrate to directly participate in the metabolic process of ammonia oxidizing bacteria, the enzymatic reaction process is shortened, and the hydroxylamine can be used as an activator of hydroxylamine redox enzyme to accelerate cell growth. In addition, since hydroxylamine inhibits the activity of nitrite oxidase under anaerobic or anoxic conditions, the addition of both in combination helps to inhibit the further conversion of nitrite nitrogen to nitrate nitrogen.

3. The invention selects the components and the proportion of the ammonia oxidizing bacteria growth promoter in the metal salt, the polyamine substance, the inorganic hydroxylamine acid and the Na₂SO₃Under the combined action of the two components, the aim of quickly culturing the ammonia oxidizing bacteria is fulfilled. The invention solves the problem of slow growth of ammonia oxidizing bacteria, and can control the nitration reaction process and improve the wastewater treatment effect. After the accelerant is used, the growth rate of the ammonia oxidizing bacteria can be increased by 10-100 times, and the nitrifying rate in the nitrifying process reaches more than 70%.

Detailed Description

The present invention will be described in detail with reference to specific examples.

The formulations of the accelerators for culturing ammonia oxidizing bacteria of examples 1 to 4 and comparative examples 1 to 6 are shown in Table 1.

TABLE 1 formulation and proportions of accelerators

The concentration of ammonia nitrogen in wastewater generated by an enterprise is 150mg/L, the wastewater is treated by an SBR process, and the accelerator is added in the startup stage of the device. The prepared accelerator with the concentration of 0.5g/L is used, the addition is carried out according to the concentration of the accelerator in the sewage of 15mg/L every day, after the addition is carried out for 15 days, the nitrosation rate in the aeration stage is increased to more than 60%, the addition is stopped, the system continues to operate for 10 days, and a water sample is taken to analyze the concentrations of the ammonia nitrogen, the nitrate nitrogen and the nitrite nitrogen in the effluent. Specific results are shown in table 2.

TABLE 2 treatment Effect with different formulations of accelerators

From the water quality analysis data of each example in table 2, in the case of no addition of the accelerator, the ammonia nitrogen concentration of the effluent is 60mg/L, the nitrosation rate is only 32%, the ammonia nitrogen concentration of the effluent after the addition of the accelerator is lower than 30mg/L, the nitrosation rate is higher than 70%, and when the accelerator only contains three or two of the ingredients, the ammonia nitrogen removal rate and the nitrosation rate of the effluent are lower than 70%. Therefore, the growth promoter can obviously improve the removal rate of ammonia nitrogen in sewage, and the nitrosation rate can reach more than 70% after the promoter is added.

Claims

1. An accelerating agent for culturing ammonia oxidizing bacteria, characterized by comprising a metal salt, a polyamine substance, hydroxylamine inorganic acid and Na₂SO₃The composition comprises, by weight, 40 to 100 parts of a metal salt, 5 to 30 parts of a polyamine substance, 0.05 to 1.5 parts of an inorganic hydroxylamine acid, and Na₂SO₃10 to 40 parts by weight; the metal salt is calcium salt, magnesium salt and copper salt, Ca²⁺、Mg²⁺And Cu²⁺The molar ratio of (5-15): (5-25): 0.5-5); the polyamine substance is spermine, spermidine or a mixture of the spermine and the spermidine; the inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate.

2. An accelerator according to claim 1 wherein: 50 to 80 parts by weight of metal salt, 10 to 20 parts by weight of polyamine,0.1 to 1.0 part by weight of inorganic hydroxylamine acid and Na₂SO₃20 to 30 parts by weight; the Ca²⁺、Mg²⁺And Cu²⁺The molar ratio of (8-12) to (10-20) to (1-4).

3. An accelerator according to claim 1 or 2, wherein: the calcium salt is CaSO₄Or CaCl₂Magnesium salt is MgSO₄Or MgCl₂The copper salt is CuSO₄Or CuCl₂。

4. An accelerator according to claim 1 or 2, wherein: the calcium salt is CaCl₂The magnesium salt is MgCl₂The copper salt being CuCl₂。

5. An accelerator according to claim 1 wherein: the inorganic hydroxylamine acid is hydroxylamine hydrochloride.

6. A method for producing an accelerator for culturing ammonia oxidizing bacteria, characterized by comprising: (1) preparing a metal salt solution according to the following components in parts by weight: 40-100 parts by weight of metal salt, wherein the metal salt is calcium salt, magnesium salt and copper salt, and Ca in the metal salt²⁺、Mg²⁺And Cu²⁺The molar ratio of (5-15): (5-25): 0.5-5); (2) 10 to 40 parts by weight of Na₂SO₃Adding into metal salt solution; (3) before use, adding 5-30 parts by weight of polyamine substances and 0.05-1.5 parts by weight of inorganic hydroxylamine acid into a metal salt solution; the polyamine substance is spermine, spermidine or a mixture of the spermine and the spermidine; the inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate.

7. The method of claim 6, wherein: 50-80 parts by weight of metal salt, wherein Ca is contained in the metal salt²⁺、Mg²⁺And Cu²⁺In a molar ratio of (8-12) to (10-20) to (1-4), Na₂SO₃20 to 30 parts by weight of polyamine10 to 20 parts by weight of a substance and 0.1 to 1.0 part by weight of an inorganic hydroxylamine acid.

8. The method of claim 6 or 7, wherein: the calcium salt is CaSO₄Or CaCl₂Magnesium salt is MgSO₄Or MgCl₂The copper salt is CuSO₄Or CuCl₂(ii) a The inorganic hydroxylamine acid is hydroxylamine hydrochloride.

9. The method of claim 8, wherein: the calcium salt is CaCl₂The magnesium salt is MgCl₂The copper salt being CuCl₂。

10. Use of an accelerator according to any one of claims 1 to 5, characterized in that: in the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 0.5-50 mg/L in the sewage.