CN105624090B

CN105624090B - Nitrifying bacteria culture promoter and preparation method thereof

Info

Publication number: CN105624090B
Application number: CN201410585430.4A
Authority: CN
Inventors: 郭志华; 高会杰; 孙丹凤; 赵胜楠; 李宝忠
Original assignee: China Petroleum and Chemical Corp; Sinopec Dalian Research Institute of Petroleum and Petrochemicals
Current assignee: Sinopec Dalian Petrochemical Research Institute Co ltd; China Petroleum and Chemical Corp
Priority date: 2014-10-28
Filing date: 2014-10-28
Publication date: 2020-06-05
Anticipated expiration: 2034-10-28
Also published as: CN105624090A

Abstract

The invention discloses a nitrobacteria culture promoter which comprises 40-100 parts by weight of metal salt, preferably 50-80 parts by weight of polyamine substance, 5-30 parts by weight of polyamine substance, preferably 10-20 parts by weight of organic acid hydroxylamine, and 0.5-15 parts by weight of organic acid hydroxylamine, preferably 2-10 parts by weight of organic acid hydroxylamine; the metal salt is calcium salt, ferrous salt, copper salt, Ca²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (1-8): 0.5-5), preferably (8-12): 2-6): 1-4. The accelerant has simple formula and easy preparation, can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system, so that the nitrobacteria can rapidly grow and reproduce in a short time, and the cultured nitrobacteria has high activity and strong impact resistance.

Description

Nitrifying bacteria culture promoter and preparation method thereof

Technical Field

The invention belongs to the technical field of biology, and particularly relates to a nitrobacteria culture promoter and a preparation method thereof.

Background

In the biological denitrification method, ammonia nitrogen is removed by nitrification of nitrifying bacteria in both traditional nitrification-denitrification, novel shortcut nitrification-denitrification and novel shortcut nitrification-anaerobic ammonia oxidation. Nitrifying bacteria belong to the group of chemolithotrophic microorganisms. Biological cells can utilize only energy stored in the form of ATP or the like, and cannot directly utilize free energy released by chemical reaction. In aerobic metabolism, ATP is synthesized mainly by oxidative phosphorylation of the respiratory chain. The ammoxidation phosphorylation efficiency is very low, the ATP generated by the ammoxidation phosphorylation is very limited, and the energy is mainly used for electron transition to a higher energy level, so that the nitrobacteria grow very slowly, and the generation period is 8-36 h. The content of peptidoglycan in the cell wall of nitrobacteria is low, and the content of protein and fat is high, so that the nitrobacteria is sensitive to environmental change, the adaptability and tolerance of natural nitrobacteria in nature are poor, and the nitrobacteria cannot compete with heterotrophic microorganisms in growth under a plurality of conditions to obtain advantages. In a sewage treatment system, when the content of nitrobacteria in activated sludge is low, the nitrobacteria cannot grow and propagate quickly in a short time by adjusting the environmental conditions such as dissolved oxygen, pH and the like, and finally the ammonia nitrogen removal capability of the existing running sewage treatment system is limited. Whether the nitrifying bacteria are cultured in the sewage treatment system directly or outside the sewage treatment system, substances for promoting growth need to be added to accelerate the growth rate of the nitrifying bacteria.

On a biochemical level, the nitration reaction is a metabolic pathway involving the co-catalysis of ammonia monooxygenase, hydroxylamine oxidoreductase and nitrite oxidase, with a complex conversion of substances and energy. The biological promoter is used for improving the activity of the enzymes in the nitrifying bacteria, so that the problem of slow growth of the nitrifying bacteria is solved, and the nitration reaction process can be accelerated. At present, many researches on biological promoters are carried out, and CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X respectively propose nitrobacteria growth promoters formed by combining different metal salts, wherein the main components comprise molasses, metal salts (ferrous salt, manganese salt, calcium salt and magnesium salt) and an adsorbent. The ammonia nitrogen removal rate can be improved by more than 20 percent after the accelerator is used. However, the addition of the adsorbents mainly comprises substances such as zeolite powder, diatomite, powdered activated carbon or fly ash and the like, which inevitably increases the sludge yield. CN201110315549.6 discloses a method for culturing shortcut nitrification and denitrification granular sludge, which is characterized in that 5-15 mg/L hydroxylamine is added periodically; CN201010168453.7 discloses a method for rapidly starting an anoxic and ammoxidation biofilter, which is characterized in that hydroxylamine is added in the step (2) to induce the inoculated sludge to be converted into an anoxic and ammoxidation biomembrane. CN201410141638.7 discloses a culture solution of nitrosobacteria and a preparation and culture method thereof, wherein the formula of the culture solution is relatively complex, and the culture solution not only relates to elements for providing cell growth such as iron, calcium, potassium, magnesium and the like, but also relates to elements for promoting enzyme synthesis such as cobalt, molybdenum, manganese, zinc and the like.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a nitrobacteria culture promoter and a preparation method thereof. The accelerant has simple formula and easy preparation, can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system, so that the nitrobacteria can rapidly grow and reproduce in a short time, and the cultured nitrobacteria has high activity and strong impact resistance.

The nitrobacteria culture promoter comprises 40-100 parts by weight of metal salt, preferably 50-80 parts by weight of polyamine substance, 5-30 parts by weight of polyamine substance, preferably 10-20 parts by weight of organic acid hydroxylamine, and 0.5-15 parts by weight of organic acid hydroxylamine, preferably 2-10 parts by weight of organic acid hydroxylamine; the metal salt is calcium salt, ferrous salt, copper salt, Ca²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (1-8): 0.5-5), preferably (8-12): 2-6): 1-4.

The calcium salt is CaSO₄Or CaCl₂Preferably CaSO₄(ii) a The ferrous salt is FeSO₄Or FeCl₂Preferably FeSO₄(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuSO₄。

The polyamine substance of the invention is spermine, spermidine or a mixture of the spermine and the spermidine.

The organic acid hydroxylamine is hydroxylamine formate, hydroxylamine acetate or a mixture of the two.

The preparation method of the nitrobacteria culture promoter comprises the following steps: (1)preparing a metal salt solution according to the following components in parts by weight: the metal salt is 40-100 parts by weight, preferably 50-80 parts by weight, the metal salt is calcium salt, ferrous salt and copper salt, and Ca in the metal salt²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (1-8): 0.5-5), preferably (8-12): 2-6): 1-4; (2) before use, 5-30 parts by weight, preferably 10-20 parts by weight of polyamine substance and 0.5-15 parts by weight, preferably 2-10 parts by weight of hydroxylamine organic acid are added into a metal salt solution.

The calcium salt in the step (1) of the invention is CaSO₄Or CaCl₂Preferably CaSO₄(ii) a The ferrous salt is FeSO₄Or FeCl₂Preferably FeSO₄(ii) a The copper salt being CuSO₄Or CuCl₂Preferably CuSO₄。

The polyamine substance in the step (2) of the invention is spermine, spermidine or a mixture of the spermine and the spermidine.

The organic acid hydroxylamines in the step (2) of the invention are hydroxylamine formate, hydroxylamine acetate or a mixture of the hydroxylamine formate and the hydroxylamine acetate.

The nitrobacteria growth promoter provided by the invention can be used in the culture process of nitrobacteria, can be used for improving the ammonia nitrogen removal effect of a sewage treatment system, and can also be used for quickly starting an ammonia-containing system and quickly recovering an impacted system. The dosage is determined according to the sewage property, the treatment capacity and the treatment effect. In the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 10-100 mg/L in the sewage.

Compared with the prior art, the invention has the following beneficial effects:

1. the metal ions provided by the metal salt are used as elements required by the growth of nitrobacteria, and simultaneously used as components of enzyme to improve the activity of the enzyme, so that the substrate can be rapidly degraded, and the enzymatic reaction process is accelerated. The polyamine substance and the metal ions act together to accelerate cell proliferation, improve the settleability and stability of the harvested thalli and prolong the service life of the thalli.

2. The added organic acid hydroxylamine can be used as a substrate of hydroxylamine redox enzyme to directly participate in the metabolic process of nitrobacteria, shorten the enzymatic reaction process and accelerate the cell growth. In addition, the organic acid released by the organic acid hydroxylamine is beneficial to the growth of some heterotrophic nitrifying bacteria, the group effect of the nitrifying bacteria is favorably exerted, and the capability of degrading pollutants by the groups of bacteria is favorably improved.

3. The invention realizes the purpose of quickly culturing nitrobacteria under the combined action of metal salt, polyamine substances and organic acid hydroxylamine by selecting the components and the proportion of the nitrobacteria growth promoter. The invention solves the problem of slow growth of nitrifying bacteria, and can accelerate the nitration reaction process and improve the treatment effect of waste water. The growth rate of nitrobacteria can be increased by 10-100 times after the accelerator is used, the ammonia nitrogen removal rate of ammonia-containing wastewater can be improved by more than 30%, and the problem of quick recovery of the nitrification deamination nitrogen capacity of an impacted sewage treatment system can be well solved.

Detailed Description

The present invention will be described in detail with reference to specific examples.

The formulations of the nitrifying bacteria growth promoting agents of examples 1 to 6 and comparative examples 1 to 7 are shown in Table 1.

TABLE 1 formulation and proportions of accelerators

When a sewage treatment plant is newly built in an enterprise, the COD concentration of main pollutants contained in the wastewater to be treated is about 200mg/L, the ammonia nitrogen concentration is about 100mg/L, a batch activated sludge process (SBR process) is adopted for startup treatment, and after two months of startup, the COD concentration in the treated effluent is less than 60mg/L, but the ammonia nitrogen concentration is still higher than 50 mg/L. The concentration of the accelerator prepared according to the proportion in the table is 0.5g/L, the accelerator is added according to the concentration of 20mg/L in the sewage every day, after 10 days of adding, the ammonia nitrogen removal rate is improved by more than 10%, the adding is stopped, the system continues to run for 10 days, and a water sample is taken to analyze the concentration of suspended matters in the effluent and the concentration of ammonia nitrogen in the effluent and calculate the ammonia nitrogen removal rate. The specific treatment results after addition are shown in Table 2.

TABLE 2 treatment Effect with different formulations of accelerators

From the quality of the effluent water in each embodiment of table 2, under the condition that no accelerator is added, the concentration of effluent suspended matters is 160mg/L, the concentration of effluent ammonia nitrogen is 50mg/L, the removal rate of ammonia nitrogen is only 50%, the concentration of the effluent suspended matters after the accelerator is added is lower than 100mg/L, the concentration of the effluent ammonia nitrogen is lower than 15mg/L, the removal rate of ammonia nitrogen is higher than 85%, when the accelerator only contains one or two of the formulas, the concentration of the effluent suspended matters is between 120 and 140mg/L, the concentration of the effluent ammonia nitrogen is still between 25 and 40mg/L, and the removal rate of ammonia nitrogen is lower than 80%. Therefore, the growth promoter can obviously improve the removal rate of ammonia nitrogen in sewage, improve the flocculation performance of sludge and reduce the concentration of effluent suspended matters.

Claims

1. A nitrobacteria culture promoter is characterized by comprising 40-100 parts by weight of metal salt, 5-30 parts by weight of polyamine substances and 0.5-15 parts by weight of organic acid hydroxylamine; the metal salt is calcium salt, ferrous salt, copper salt, Ca²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (1-8): 0.5-5); the polyamine substance is spermine, spermidine or a mixture of spermine and spermidine; the organic acid hydroxylamine is hydroxylamine formate, hydroxylamine acetate or a mixture of the two.

2. The culture promoter according to claim 1, wherein: 50-80 parts of metal salt, 10-20 parts of polyamine substance, 2-10 parts of organic acid hydroxylamine, and Ca²⁺、Fe²⁺And Cu²⁺The molar ratio of (8-12) to (2-6) to (1-4).

3. The culture promoter according to claim 1 or 2, wherein: the calcium salt being CaSO₄Or CaCl₂The ferrous salt is FeSO₄Or FeCl₂The copper salt is CuSO₄Or CuCl₂。

4. The culture promoter according to claim 1 or 2, wherein: the calcium salt is CaSO₄The ferrous salt is FeSO₄The copper salt is CuSO₄。

5. A preparation method of a nitrobacteria culture promoter is characterized by comprising the following steps: (1) preparing a metal salt solution according to the following components in parts by weight: 40-100 parts of metal salt, wherein the metal salt is calcium salt, ferrous salt and copper salt, and Ca in the metal salt²⁺、Fe²⁺And Cu²⁺The molar ratio of (5-15): (1-8): 0.5-5); (2) before use, adding 5-30 parts by weight of polyamine substances and 0.5-15 parts by weight of hydroxylamine organic acid into a metal salt solution; the polyamine substance is spermine, spermidine or a mixture of spermine and spermidine; the organic acid hydroxylamine is hydroxylamine formate, hydroxylamine acetate or a mixture of the two.

6. The method of claim 5, wherein: 50-80 parts by weight of metal salt, wherein Ca is contained in the metal salt²⁺、Fe²⁺And Cu²⁺The molar ratio of (8-12) to (2-6) to (1-4); before use, 10-20 parts by weight of polyamine substance and 2-10 parts by weight of organic acid hydroxylamine are added into a metal salt solution.

7. The method of claim 5, wherein: the calcium salt in the step (1) is CaSO₄Or CaCl₂The ferrous salt is FeSO₄Or FeCl₂The copper salt is CuSO₄Or CuCl₂。

8. The method of claim 7, wherein: the calcium salt is CaSO₄The ferrous salt is FeSO₄The copper salt is CuSO₄。

9. Use of the culture promoter according to any one of claims 1 to 4, wherein: in the using process, firstly, the accelerant is dissolved, and then the accelerant is added according to the concentration of 10-100 mg/L in the sewage.