CN108118021B

CN108118021B - Microbial growth promoter for completing nitration process and application thereof

Info

Publication number: CN108118021B
Application number: CN201611072922.9A
Authority: CN
Inventors: 高会杰; 孙丹凤; 郭志华; 赵胜楠; 陈明翔
Original assignee: China Petroleum and Chemical Corp; Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Current assignee: China Petroleum and Chemical Corp; Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Priority date: 2016-11-29
Filing date: 2016-11-29
Publication date: 2020-11-10
Anticipated expiration: 2036-11-29
Also published as: CN108118021A

Abstract

The present invention relates to a microbial growth promoter for accomplishing a nitrification process, comprising at least one glycolipid selected from the group consisting of algal glycolipids, sophorolipids and rhamnolipids, preferably sophorolipids, wherein sophorolipids are both esterified and acid type, more preferably acid type sophorolipids. The invention provides the application of the microbial growth promoter, which can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system comprising a nitrification unit for improving the ammonia nitrogen removal effect of the sewage treatment system; and can also be used for quick start of an ammonia-containing system and quick recovery of an impacted system. The accelerant has simple formula and easy preparation, can lead the nitrobacteria with slow growth to rapidly grow and reproduce in a short time when being used for the nitration process, and the cultured thalli has the characteristics of high activity, good flocculation, long service time, strong impact resistance and the like.

Description

Microbial growth promoter for completing nitration process and application thereof

Technical Field

The invention belongs to the technical field of microorganisms, and particularly relates to a microbial growth promoter for completing a nitration process and application thereof.

Background

In the biological denitrification method, ammonia nitrogen is removed by nitrification of nitrifying bacteria in both traditional nitrification-denitrification, novel shortcut nitrification-denitrification and novel shortcut nitrification-anaerobic ammonia oxidation. Nitrifying bacteria belong to chemotrophic microorganisms, and biological cells can only utilize energy stored in forms of ATP and the like, but cannot directly utilize free energy released by chemical reaction. In aerobic metabolism, ATP is synthesized mainly by oxidative phosphorylation of the respiratory chain. The efficiency of ammoxidation and phosphorylation is low, the ATP produced is very limited, and the energy is mainly used for electron transition to higher energy level, so that the nitrobacteria grow slowly with the generation period of 8-36 h. The content of peptidoglycan in the cell wall of nitrobacteria is low, and the content of protein and fat is high, so that the nitrobacteria is sensitive to environmental change, the adaptability and tolerance of natural nitrobacteria in nature are poor, and the nitrobacteria cannot compete with heterotrophic microorganisms in growth under a plurality of conditions to obtain advantages. In a sewage treatment system, when the content of nitrobacteria in activated sludge is low, the nitrobacteria cannot grow and propagate quickly in a short time by adjusting the environmental conditions such as dissolved oxygen, pH and the like, and finally the ammonia nitrogen removal capability of the existing running sewage treatment system is limited. The growth of the nitrifying bacteria is slow and the culture period is long no matter the nitrifying bacteria are directly cultured in the sewage treatment system or are cultured outside the sewage treatment system. On a biochemical level, the nitration reaction is a metabolic pathway involving the co-catalysis of ammonia monooxygenase, hydroxylamine oxidoreductase and nitrite oxidase, with a complex conversion of substances and energy. The biological promoter is used for improving the activity of the enzymes in the nitrifying bacteria, so that the problem of slow growth of the nitrifying bacteria is solved, and the nitration reaction process can be accelerated.

At present, many researches on biological promoters are carried out, and Chinese patents CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X respectively propose nitrifying bacteria growth promoters formed by combining different metal salts, wherein the main components comprise molasses, metal salts (iron salt, manganese salt, calcium salt and magnesium salt) and an adsorbent. The ammonia nitrogen removal rate can be improved by more than 20 percent after the accelerator is used. However, the addition of the adsorbents mainly comprises substances such as zeolite powder, diatomite, powdered activated carbon or fly ash and the like, which inevitably increases the sludge yield. CN200680027611.9 discloses a growth promoter and a life prolonging agent, which are mainly used as growth promoters and life prolonging agents for farm animals, cultured microorganisms, cultivated plants or mushrooms, and have growth promoting effects, proliferation effects, survival rate improving effects, life prolonging effects, repairing effects, etc. when sugar alcohols such as animals, plants, microorganisms, mushrooms, etc., especially erythritol are administered. The invention does not relate to the microorganism culture in the field of wastewater treatment, even if the invention is suitable for the field of wastewater denitrification, due to the broad spectrum, the invention can promote the growth of nitrobacteria and other microorganisms and mixed bacteria at the same time, and the specificity for promoting the growth of nitrobacteria is not good.

The nitrifying bacteria promoters disclosed in Chinese patents CN201410585418.3, CN201410585417.9, CN201410585482.1, CN201410585430.4 and the like mainly comprise metal salts and polyamine substances, and can promote the growth of nitrifying microorganisms and improve the treatment effect. But the metal salt content in the accelerant is higher, and the secondary pollution of sewage can be caused after the accelerant is added for a long time.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a microbial growth promoter for completing a nitration process and application thereof. The accelerant has simple formula and easy preparation, can lead the nitrobacteria with slow growth to rapidly grow and reproduce in a short time when being used for the nitration process, and the cultured thalli has the characteristics of high activity, good flocculation, long service time, strong impact resistance and the like.

The microbial growth promoter for accomplishing the nitrification process of the present invention comprises at least one glycolipid selected from the group consisting of algal glycolipids, sophorolipids, rhamnolipids, and the like, preferably sophorolipids, wherein sophorolipids are both of an esterified type and an acid type, more preferably an acid type sophorolipids.

Further, the accelerant also comprises sugar alcohol, wherein the sugar alcohol is selected from one or more of mannitol, xylitol, lactitol, ribitol, galactitol, inositol, erythritol and the like, and preferably the lactitol.

In the accelerator of the present invention, the amount of sugar alcohol is such that the mass ratio of glycolipid to sugar alcohol is 1:1 to 1: 5.

The application of the microbial growth promoter can be used in the culture process of nitrobacteria, and can also be directly added into a sewage treatment system comprising a nitrification unit for improving the ammonia nitrogen removal effect of the sewage treatment system; and can also be used for quick start of an ammonia-containing system and quick recovery of an impacted system. The dosage and the use mode are determined according to the sewage property, the treatment capacity and the treatment effect. During the use process, the accelerant is firstly dissolved and then added according to the concentration of the accelerant in the sewage of 0.01-1mg/L, preferably 0.05-0.5mg/L every day. The temperature is controlled to be 20-40 ℃, the pH value is 6.0-9.0, and the dissolved oxygen is 1.0-5.0mg/L in the treatment process.

In the application of the microbial growth promoter, a certain amount of auxiliary agent is added simultaneously in the using process, wherein the auxiliary agent can be one or two of polyamine substances and inorganic hydroxylamine acid, and preferably two substances are added simultaneously. Wherein the polyamine is spermine, spermidine or their mixture, and the inorganic acid hydroxylamine is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate. The addition amount of the auxiliary agent is 0.1-0.5 time of the concentration of the accelerator. The addition of the auxiliary agent provides elements required for the growth of bacteria, simultaneously improves the activity of enzyme as an enzyme component, directly participates in the metabolic process of bacteria as a substrate of hydroxylamine oxidoreductase, shortens the enzymatic reaction process, and further improves the thallus performance and the sewage treatment effect under the synergistic effect with glycolipid and sugar alcohol.

The growth promoter of the invention takes glycolipid as a main component, and can realize the purpose of quickly culturing nitromicroorganisms under the combined action of the glycolipid, the sugar alcohol and the like. The cultured nitrifying microorganisms have high activity, good flocculation, long service time, strong impact resistance and high salt tolerance. When the microbial carrier is used for biochemical treatment, the microbial carrier is beneficial to exerting the colony effect of microorganisms, improving the capability of resisting the harmful environments such as high salt and the like by the microorganisms and improving the capability of degrading pollutants.

The sophorolipid used in the accelerant has good environmental and biological compatibility, namely has the characteristics of no toxicity, no harm and easy degradation, can increase the contact area of microorganisms and a matrix, improves the mass transfer efficiency, further improves the growth rate and the biological activity of organisms, and cannot cause secondary pollution.

The invention solves the problem of slow growth of nitrifying bacteria, and can accelerate the nitration reaction process and improve the treatment effect of waste water. The growth rate of nitrobacteria can be increased by 10-100 times after the accelerator is used, the removal rate of ammonia nitrogen in the ammonia-containing wastewater can be improved by more than 30%, and the problem of quick recovery of the nitrification deamination nitrogen capacity of an impacted sewage treatment system can be well solved.

Detailed Description

The present invention will be described in detail with reference to specific examples, but the scope of the present invention is not limited to the examples described above, which are only for better understanding of the present invention.

The formulation of the microbial growth promoting agent used in the examples of the present invention is specifically shown in table 1.

TABLE 1 composition and proportions of accelerators

Example 1

The COD concentration of main pollutants contained in the wastewater discharged by a certain factory is about 400mg/L, the ammonia nitrogen concentration is about 150mg/L, the factory adopts a batch activated sludge process (SBR process) for treatment, and the COD concentration in the treated effluent is less than 60mg/L, but the ammonia nitrogen concentration is still higher than 50 mg/L. The accelerant prepared in the table 1 is added into the treatment system, the adding is carried out every day according to the concentration of the accelerant in the sewage being 0.5mg/L, after the adding is carried out for 15 days, the ammonia nitrogen removal rate is improved by more than 10%, the adding is stopped, the system continues to operate for 10 days, a water sample is taken to analyze the ammonia nitrogen concentration of effluent water and calculate the ammonia nitrogen removal rate, and the specific treatment effect is shown in the table 2.

TABLE 2 treatment Effect with different accelerators

As can be seen from the data in Table 2, the ammonia nitrogen concentration of the effluent is lower than 15mg/L after the accelerator is used, and the ammonia nitrogen removal rate is higher than 70%. The growth promoter can obviously improve the removal effect of ammonia nitrogen in sewage.

Example 2

The processing technique and the operating conditions are the same as those of the example 1, except that: a certain amount of the auxiliary agent is added while the No. 1 accelerator is added, and the composition, the adding amount and the effect of the auxiliary agent are shown in Table 3.

TABLE 3 treatment effect by adding different adjuvants simultaneously

As can be seen from Table 3, the ammonia nitrogen concentration is further reduced to less than 10mg/L by the addition of the auxiliary agent, the removal rate is more than 80%, and the treatment effect is further improved.

Claims

1. A microbial growth promoter which accomplishes a nitrification process, characterized by comprising at least one glycolipid selected from the group consisting of algal glycolipids, sophorolipids and rhamnolipids; the sugar alcohol is selected from one or more of mannitol, xylitol, lactitol, ribitol, galactitol, inositol and erythritol; the mass ratio of glycolipid to sugar alcohol is 1:1-1: 5.

2. The accelerator according to claim 1, characterized in that: the sophorolipid is an acid sophorolipid.

3. Use of a microbial growth promoter according to claim 1 or 2, characterized in that: the accelerant is used in the culture process of nitrifying bacteria, or is directly added into a sewage treatment system comprising a nitrifying unit, and is used for improving the ammonia nitrogen removal effect of the sewage treatment system; or for rapid start-up of the ammonia-containing system and rapid recovery of the impacted system.

4. Use of an accelerator according to claim 3, characterized in that: during the use process, the accelerant is firstly dissolved and then added according to the concentration of the accelerant in the sewage of 0.01-1mg/L every day.

5. Use of an accelerator according to claim 3, characterized in that: in the using process, the temperature is controlled to be 20-40 ℃, the pH is controlled to be 6.0-9.0, and the dissolved oxygen is 1.0-5.0 mg/L.

6. Use of an accelerator according to claim 3 or 4, characterized in that: during the use process, a certain amount of auxiliary agent is added at the same time, and the auxiliary agent is one or two of polyamine substances and inorganic hydroxylamine acid.

7. Use according to claim 6, characterized in that: the polyamine substance is spermine, spermidine or a mixture of spermine and spermidine; the inorganic hydroxylamine acid is one or more of hydroxylamine hydrochloride, hydroxylamine sulfate or hydroxylamine phosphate.

8. Use according to claim 6, characterized in that: the addition amount of the auxiliary agent is 0.1-0.5 time of the concentration of the accelerator.