CN109942080B - Composition for promoting denitrification of anaerobic ammonium oxidation bacteria and application thereof - Google Patents
Composition for promoting denitrification of anaerobic ammonium oxidation bacteria and application thereof Download PDFInfo
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- CN109942080B CN109942080B CN201711391757.8A CN201711391757A CN109942080B CN 109942080 B CN109942080 B CN 109942080B CN 201711391757 A CN201711391757 A CN 201711391757A CN 109942080 B CN109942080 B CN 109942080B
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- 239000000203 mixture Substances 0.000 title claims abstract description 41
- 230000003647 oxidation Effects 0.000 title claims abstract description 20
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 20
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title claims abstract description 17
- 230000001737 promoting effect Effects 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 28
- 229930186217 Glycolipid Natural products 0.000 claims abstract description 12
- ZTOKUMPYMPKCFX-CZNUEWPDSA-N (E)-17-[(2R,3R,4S,5S,6R)-6-(acetyloxymethyl)-3-[(2S,3R,4S,5S,6R)-6-(acetyloxymethyl)-3,4,5-trihydroxyoxan-2-yl]oxy-4,5-dihydroxyoxan-2-yl]oxyoctadec-9-enoic acid Chemical compound OC(=O)CCCCCCC/C=C/CCCCCCC(C)O[C@@H]1O[C@H](COC(C)=O)[C@@H](O)[C@H](O)[C@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](COC(C)=O)O1 ZTOKUMPYMPKCFX-CZNUEWPDSA-N 0.000 claims abstract description 9
- HVCOBJNICQPDBP-UHFFFAOYSA-N 3-[3-[3,5-dihydroxy-6-methyl-4-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyoxan-2-yl]oxydecanoyloxy]decanoic acid;hydrate Chemical compound O.OC1C(OC(CC(=O)OC(CCCCCCC)CC(O)=O)CCCCCCC)OC(C)C(O)C1OC1C(O)C(O)C(O)C(C)O1 HVCOBJNICQPDBP-UHFFFAOYSA-N 0.000 claims abstract description 9
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims abstract description 9
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004021 humic acid Substances 0.000 claims abstract description 6
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 claims abstract 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 5
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 235000010331 calcium propionate Nutrition 0.000 claims description 4
- 239000004330 calcium propionate Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- 239000002253 acid Substances 0.000 claims description 2
- 239000011575 calcium Substances 0.000 claims description 2
- 229910052791 calcium Inorganic materials 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- BWILYWWHXDGKQA-UHFFFAOYSA-M potassium propanoate Chemical compound [K+].CCC([O-])=O BWILYWWHXDGKQA-UHFFFAOYSA-M 0.000 claims description 2
- 235000010332 potassium propionate Nutrition 0.000 claims description 2
- 239000004331 potassium propionate Substances 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 claims description 2
- 235000010334 sodium propionate Nutrition 0.000 claims description 2
- 239000004324 sodium propionate Substances 0.000 claims description 2
- 229960003212 sodium propionate Drugs 0.000 claims description 2
- XDWXRAYGALQIFG-UHFFFAOYSA-L zinc;propanoate Chemical compound [Zn+2].CCC([O-])=O.CCC([O-])=O XDWXRAYGALQIFG-UHFFFAOYSA-L 0.000 claims description 2
- 241001474374 Blennius Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 238000002360 preparation method Methods 0.000 abstract description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract description 2
- -1 trehalose ester Chemical class 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 239000010802 sludge Substances 0.000 description 8
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 7
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 150000002596 lactones Chemical class 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000027756 respiratory electron transport chain Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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Abstract
The invention relates to a composition for promoting denitrification of anaerobic ammonium oxidation bacteria and application thereof, wherein the composition comprises glycolipid, propionate, humic acid or/and humate, wherein the glycolipid is at least one of algal glycolipid, sophorolipid, rhamnolipid and the like, and preferably trehalose ester. The composition has simple formula and easy preparation, and can improve the denitrification activity of the anaerobic ammonium oxidation bacteria and promote the smooth operation of the denitrification process when being used in the anaerobic ammonium oxidation process.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a composition capable of promoting denitrification of anammox bacteria and application thereof.
Background
With the development of social economy and the improvement of the living standard of people, the content of ammonia nitrogen in wastewater discharged in industry and life is higher and higher, the eutrophication of water bodies is continuously generated, and the problem of nitrogen pollution is attracted by people. The traditional biological denitrification method mainly comprises nitrification-denitrification, but is not suitable for treating sewage with low carbon-nitrogen ratio due to the dependence on organic carbon sources. With the discovery of a novel biological denitrification way, a novel biological denitrification process is continuously developed and utilized.
Anammox (Anammox) is a new biological denitrification technology developed in recent years, which refers to a microbial reaction that converts ammonia and nitrite into nitrogen under anaerobic conditions with ammonia as an electron donor and nitrite as an electron acceptor. Compared with the traditional biological denitrification technology, the process has the advantages of high treatment efficiency, no need of an additional organic carbon source as an electron donor, low operation cost and the like, and has wide development prospect. Microorganisms capable of anammox reactions, called anammox bacteria, grow slowly, have low cell yields, and are very sensitive to environmental conditions, limiting the application of this technology.
CN105923795A discloses a method for rapidly culturing anammox bacteria, which is characterized in that the effluent of a conventional anammox reactor is used as the inlet water of the anammox bacteria culture reactor, ammonia nitrogen and nitrite nitrogen are added into the inlet water, activated sludge is used as the inoculum of the anammox bacteria culture reactor, and the anammox bacteria with high denitrification efficiency and stable treatment effect are cultured within 5-90 days by optimizing and controlling the composition of a matrix and the operation parameters. The method realizes rapid culture by optimizing and controlling the composition of the matrix and the operation parameters, has complex adjustment process and longer operation time, and needs to add a certain amount of nutrient substances and trace elements into inlet water to ensure that the total concentration of metal cations is 4-5 g/L and the adding amount is large.
CN106011017A discloses a method for realizing rapid proliferation of anammox bacteria, which is characterized in that based on the maximum electron transfer capacity of anammox bacteria, the maximum electron transfer capacity is combined with an MBR reactor to start anammox, and mixed sludge of nitrified sludge (90%) and anaerobic granular sludge (10%) is inoculated to gradually shorten the retention time of the sludge, improve the maximum specific growth rate of the anammox bacteria and realize the rapid proliferation of the anammox bacteria. The invention combines MBR and anaerobic ammonia oxidation together to inoculate an optimal sludge source. However, since the two bacteria have different suitable growth environments, long-term operation is not favorable for maintaining the activity of the bacteria.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a composition capable of promoting the denitrification of anammox bacteria and application thereof. The composition has simple formula and easy preparation, and can improve the denitrification activity of the anaerobic ammonium oxidation bacteria and promote the smooth operation of the denitrification process when being used in the anaerobic ammonium oxidation process.
The composition for promoting the denitrification of the anaerobic ammonium oxidation bacteria comprises glycolipids, propionate, humic acid or/and humate, wherein the glycolipids are at least one of trehalose glycolipids, sophorolipids, rhamnolipids and the like, and trehalose esters are preferred.
The sophorolipid is lactone type or acid type, preferably lactone type sophorolipid.
The humate is at least one of sodium humate, potassium humate, magnesium humate, calcium humate and the like, and the potassium humate is preferred.
The propionate is at least one of sodium propionate, potassium propionate, zinc propionate, calcium propionate and the like, and calcium propionate is preferred.
The glycolipid content is 0.5-15 parts by weight, preferably 2-10 parts by weight; the propionate content is 0.5-5 parts by weight, preferably 1-3 parts by weight; the content of humic acid or/and humate is 5-30 weight parts, preferably 10-20 weight parts.
The invention also provides a culture medium capable of promoting the denitrification of the anammox bacteria, which contains ammonia nitrogen, nitrite and the composition.
The invention also provides a method for culturing the anaerobic ammonium oxidation bacteria, which comprises the step of contacting a culture system containing the anaerobic ammonium oxidation bacteria with the composition or inoculating the anaerobic ammonium oxidation bacteria into the culture medium for culturing.
The invention also provides a method for denitrification with anammox bacteria comprising contacting a denitrification system comprising anammox bacteria with the composition.
In the culture method or the denitrification method provided by the invention, the molar ratio of ammonia nitrogen to nitrite is 1:1-1: 2; the dosage of the composition is 0.01-10mg/L, preferably 0.5-1.0 mg/L.
In the culture method or denitrification method provided by the invention, the contact conditions are as follows: the temperature is 20-35 ℃, the pH is 7-9, and the dissolved oxygen is less than 1 mg/L.
By adopting the technical scheme, the growth of the anammox bacteria can be promoted, so that the denitrification activity of the anammox bacteria is improved, the smooth denitrification process is promoted, and the treatment time is shortened. The anaerobic ammonium oxidation bacteria cultured by the composition has high activity, good flocculation property, long service time and strong impact resistance. Moreover, the composition disclosed by the invention is simple in formula, easy to prepare, environment-friendly and economical, and cannot cause secondary pollution to water.
Detailed Description
The preparation and effects of the present invention are further illustrated by the following examples. The embodiments are implemented on the premise of the technical scheme of the invention, and detailed implementation modes and specific operation processes are given, but the protection scope of the invention is not limited by the following embodiments.
The experimental procedures in the following examples are, unless otherwise specified, conventional in the art. The experimental materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
In the present invention, the contacting conditions are: the temperature is 20-35 ℃, the pH is 7-9, and the dissolved oxygen is less than 1 mg/L. Wherein the dissolved oxygen is measured by iodometry.
In the invention, the anammox bacteria can be various anammox bacteria and the like which are common in the field, and can carry out denitrification reaction by taking nitrite and ammonia nitrogen as substrates. The anaerobic ammonium oxidation bacteria granular sludge domesticated and cultured in a laboratory is used in the implementation.
In the following examples, COD concentration was measured by the dichromate method, GB11914-89 "determination of Water quality-chemical oxygen demand"; the ammonia nitrogen concentration is measured by GB7478-87 water quality-ammonium measurement-distillation and titration method; the nitrite nitrogen concentration is measured by GB7493-87 water quality-nitrite nitrogen determination-spectrophotometry; the total nitrogen concentration adopts GB 11894-89 'determination of water quality-total nitrogen-alkaline potassium persulfate digestion ultraviolet spectrophotometry'.
Compositions # 1 to # 23 were formulated according to the compositions and proportions of table 1.
TABLE 1 formulation and proportions of the compositions
The COD concentration in certain wastewater is 100mg/L, the ammonia nitrogen concentration is 100mg/L, and the nitrite nitrogen concentration is 100 mg/L. Adopting an anaerobic ammonia oxidation method for treatment, wherein the initial adding amount of the anaerobic ammonia oxidation sludge is MLSS =5000mg/L, the temperature is 30-35 ℃, the pH is 7.0-8.0 in the treatment process, and the dissolved oxygen is less than 1 mg/L. After treatment, COD in the effluent is 80mg/L, the ammonia nitrogen concentration is more than 50mg/L, the nitrite nitrogen concentration is more than 55mg/L, and the total nitrogen of the effluent is more than 110 mg/L.
In order to improve the denitrification effect of anammox bacteria, the compositions prepared in Table 1 were added to the treatment system, and the compositions were numbered as example 1 to example 17 and comparative examples 1 to 7. The composition is added according to the concentration of 0.5mg/L in the sewage treatment system every day, 20 days after the addition, the COD, ammonia nitrogen, nitrite nitrogen and total nitrogen concentration of effluent are sampled and analyzed, and the results are shown in Table 2.
TABLE 2
From the data in Table 2, the ammonia nitrogen concentration of the effluent after the composition is used is lower than 30mg/L, the nitrite nitrogen concentration is lower than 30mg/L, and the total nitrogen concentration is lower than 60 mg/L. When any one of the composition formulas is absent, the ammonia nitrogen concentration and the nitrite nitrogen concentration are both higher than 40mg/L, and the total nitrogen concentration of effluent is both higher than 90 mg/L.
Claims (16)
1. A composition for promoting denitrification of anaerobic ammonium oxidation bacteria is characterized in that: the composition comprises glycolipid, propionate and humic acid or/and humate, wherein the glycolipid is at least one of seaweed glycolipid, sophorolipid and rhamnolipid; the propionate is at least one of sodium propionate, potassium propionate, zinc propionate and calcium propionate; the glycolipid content is 0.5-15 parts by weight, the propionate content is 0.5-5 parts by weight, and the humic acid or/and humate content is 5-30 parts by weight.
2. The composition for promoting denitrification of anammox bacteria according to claim 1, wherein: the sophorolipid is lactone-type sophorolipid or/and acid-type sophorolipid.
3. The composition for promoting denitrification of anammox bacteria according to claim 2, wherein: the sophorolipid is an acid sophorolipid.
4. The composition for promoting denitrification of anammox bacteria according to claim 1, wherein: the humate is at least one of sodium humate, potassium humate, magnesium humate and calcium humate.
5. The composition for promoting denitrification of anammox bacteria according to claim 4, wherein: the humate is potassium humate.
6. The composition for promoting denitrification of anammox bacteria according to claim 1, wherein: the propionate is calcium propionate.
7. The composition for promoting denitrification of anammox bacteria according to any one of claims 1-6, wherein: the glycolipid content is 2-10 parts by weight; the content of propionate is 1-3 weight parts; the content of humic acid or/and humate is 10-20 weight portions.
8. A culture medium capable of promoting denitrification of anaerobic ammonium oxidation bacteria is characterized in that: the culture medium comprising ammonia nitrogen and nitrite, and a composition according to any one of claims 1 to 7.
9. A method for culturing anaerobic ammonium oxidation bacteria is characterized in that: a culture system containing anammox bacteria is contacted with the composition of any one of claims 1 to 7, or anammox bacteria is inoculated into the culture medium of claim 8 for culture.
10. A method for denitrification by using anaerobic ammonium oxidation bacteria is characterized in that: contacting a denitrification system comprising anammox bacteria with the composition of any of claims 1-7.
11. The method for culturing anammox bacteria according to claim 9, wherein: the dosage of the composition is 0.01-10 mg/L.
12. The method of culturing anammox bacteria according to claim 11, wherein: the dosage of the composition is 0.5-1.0 mg/L.
13. The method for denitrification with anammox bacteria according to claim 10, wherein: the dosage of the composition is 0.01-10 mg/L.
14. The method for denitrification with anammox bacteria according to claim 13, wherein: the dosage of the composition is 0.5-1.0 mg/L.
15. The method for culturing anammox bacteria according to claim 9, wherein: the contact conditions are as follows: the temperature is 20-35 ℃, the pH is 7-9, and the dissolved oxygen is less than 1 mg/L.
16. The method for denitrification with anammox bacteria according to claim 10, wherein: the contact conditions are as follows: the temperature is 20-35 ℃, the pH is 7-9, and the dissolved oxygen is less than 1 mg/L.
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