CN109880784B - Composition for promoting denitrification of denitrifying microorganisms and application thereof - Google Patents
Composition for promoting denitrification of denitrifying microorganisms and application thereof Download PDFInfo
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- CN109880784B CN109880784B CN201711279578.5A CN201711279578A CN109880784B CN 109880784 B CN109880784 B CN 109880784B CN 201711279578 A CN201711279578 A CN 201711279578A CN 109880784 B CN109880784 B CN 109880784B
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Abstract
The invention relates to a composition for promoting denitrification of denitrifying microorganisms and application thereof, wherein the composition comprises glycolipid, organic acid salt, humic acid or/and humate, wherein the glycolipid is at least one of algal glycolipid, sophorolipid, rhamnolipid, celloglycolipid and the like, and preferably the algal glycolipid is trehalose ester. The composition has simple formula and easy preparation, and can improve the denitrification activity of denitrifying microorganisms and promote the smooth operation of the denitrification process when being used in the denitrification process.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a composition for promoting denitrification of denitrifying microorganisms and application thereof.
Background
Denitrifying bacteria are a type of facultative anaerobic microorganisms that convert nitrate or nitrite nitrogen into nitrogen. The microorganisms can induce nitrate reductase A and nitrite reductase required by denitrification only under anaerobic condition, and nitrate or nitrite is taken as an electron acceptor for denitrification. Because of different environments required by microorganisms, the impact resistance of the system is poor in the whole denitrification process of sewage treatment, namely the nitrification process and the denitrification process, high-concentration ammonia nitrogen and nitrate nitrogen can inhibit the growth of nitrifying bacteria, and high-concentration dissolved oxygen and carbon source deficiency can inhibit the growth of denitrifying bacteria, so that the total denitrification effect is influenced. However, there is no method for effectively obtaining denitrifying bacteria, which is used for sewage treatment to perform nitrification and denitrification processes.
In recent years, loss of total nitrogen has been frequently found in many aerobic denitrification tanks in actual operation (experimental studies on simultaneous nitrification and denitrification, von willebrand disease, and the like, Shanghai environmental science, 2002, 21). Many studies and reports at home and abroad also confirm the existence of aerobic denitrification (Ding ai, moderate, experimental evidence of aerobic biological denitrification reaction [ J ], scientific report, 2000, 45). Therefore, the denitrification technology is developed by using the aerobic denitrifying bacteria, the denitrification process can be carried out in the same reactor, and the occupied area and the construction investment are greatly reduced; the dosage of chemical substances and carbon sources for adjusting the pH of the system can be reduced, the medicament cost is saved, and the running cost is reduced; COD (chemical oxygen demand) can be removed while denitrification is carried out, the shock resistance of the system is enhanced, and the treatment effect is good.
CN1800053A discloses a method for denitrifying nitrite by using granular sludge, which has the advantages of high sludge concentration in a system, high reactor efficiency, simple process and the like, but the denitrifying granular sludge can be obtained only after being cultured for 30-90 days at 15-30 ℃, and the concentration of nitrite nitrogen in the treated wastewater is 10-200 mg/L. CN101898828A discloses a method for culturing shortcut denitrification granular sludge, which is to realize enrichment culture of denitrification granular sludge in three stages by selecting different culture matrixes. CN105621611A discloses a denitrifying bacterial agent for denitrification by nitrite and application thereof, the invention mainly adopts strains with special combination and proportion to realize denitrification, relates to a plurality of strains, and has complex preparation process. CN106754450A describes a denitrifying microorganism culture promoter, which mainly comprises metal salts, polyamine substances, organic acid hydroxylamine and organic acid salts, and can promote the growth of denitrifying microorganisms and improve the treatment effect, but the promoter has high content of metal salts, and can cause secondary pollution to water after long-term addition.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a composition for promoting denitrification of denitrifying microorganisms and application thereof. The composition has simple formula and easy preparation, and can improve the denitrification activity of denitrifying microorganisms and promote the smooth operation of the denitrification process when being used in the denitrification process.
The composition for promoting denitrification of denitrifying microorganisms provided by the invention comprises glycolipids, organic acid salt, humic acid or/and humate, wherein the glycolipids are at least one of algal glycolipids, sophorolipids, rhamnolipids, celloglycolipids and the like, and preferably the algal glycolipids are trehalose esters.
The sophorolipid is lactone type or acid type, preferably lactone type sophorolipid.
The humate is at least one of sodium humate, potassium humate, magnesium humate, calcium humate and the like, and the potassium humate is preferred.
The organic acid salt is at least one of organic acid salts such as sodium acetate, sodium succinate and sodium citrate, and preferably sodium succinate.
The glycolipid content is 0.5-15 parts by weight, preferably 2-10 parts by weight; the organic acid salt is 0.5 to 15 weight parts, preferably 2 to 10 weight parts; the content of humic acid or/and humate is 5-30 weight parts, preferably 10-20 weight parts.
Further, the composition further comprises ascorbic acid, added in an amount of 1% -45%, preferably 10% -30% by weight of the glycolipid.
The invention also provides a culture medium capable of promoting denitrification of denitrifying microorganisms, which contains nitrate and/or nitrite, and the composition.
The invention also provides a method for culturing denitrifying microorganisms, which comprises contacting a culture system containing denitrifying microorganisms with the composition, or inoculating denitrifying microorganisms into the culture medium for culturing.
The invention also provides a method for denitrification using denitrifying microorganisms, comprising contacting a denitrification system containing denitrifying microorganisms with a composition.
In the culture method or denitrification method provided by the invention, the dosage of the composition is 0.01-10mg/L, preferably 0.5-1.0 mg/L. The contact conditions are as follows: the temperature is 20-40 deg.C, pH is 6-9, and dissolved oxygen is 0.1-3 mg/L.
By adopting the technical scheme, the growth of the denitrifying microorganisms can be promoted, so that the denitrification activity of the denitrifying microorganisms is improved, the smooth proceeding of the denitrifying denitrification process is promoted, and the treatment time is shortened. The denitrifying microorganism cultured by using the composition has high activity, good flocculation property, long service time and strong impact resistance. Moreover, the composition disclosed by the invention is simple in formula, easy to prepare, environment-friendly and economical, and cannot cause secondary pollution to water.
Detailed Description
The preparation and effects of the present invention are further illustrated by the following examples. The embodiments are implemented on the premise of the technical scheme of the invention, and detailed implementation modes and specific operation processes are given, but the protection scope of the invention is not limited by the following embodiments.
The experimental procedures in the following examples are, unless otherwise specified, conventional in the art. The experimental materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified.
In the present invention, the contacting conditions are: the temperature is 20-40 deg.C, pH is 6-9, and dissolved oxygen is 0.1-3 mg/L. Wherein the dissolved oxygen is measured by iodometry.
In the invention, the denitrifying microorganism can be various denitrifying microorganisms (usually bacteria) commonly used in the field, such as denitrifying microorganisms (usually bacteria) taken from a certain sewage treatment plant, and the total nitrogen removal rate is more than 80%. Denitrifying microorganisms achieve denitrification by respiration (respiration) using nitrate and/or nitrite as electron acceptors, and thus, the nitrate and/or nitrite can be a variety of common inorganic nitrogen sources that provide electron acceptors for denitrifying microorganisms.
In the present invention, the present invention will be described in detail below by way of examples. In the following examples, COD concentration was measured by the dichromate method, GB11914-89 "determination of Water quality-chemical oxygen demand"; the ammonia nitrogen concentration is measured by GB7478-87 water quality-ammonium measurement-distillation and titration method; the nitrite nitrogen concentration is measured by GB7493-87 water quality-nitrite nitrogen determination-spectrophotometry; the total nitrogen concentration adopts GB 11894-89 'determination of water quality-total nitrogen-alkaline potassium persulfate digestion ultraviolet spectrophotometry'.
Compositions # 1 to # 24 were formulated according to the compositions and proportions of table 1.
TABLE 1 formulation and proportions of the compositions
COD concentration in wastewater generated by a certain enterprise is 450mg/L and ammonia nitrogen concentration is 150mg/L, after the wastewater is treated by a nitrification and denitrification process, the average COD concentration in effluent is 90mg/L and the ammonia nitrogen concentration (NH)3-N) below 15mg/L, total nitrogen concentration (TN) up to 70mg/L, of which nitrite Nitrogen (NO)2 -N) concentration was 40 mg/L. The temperature is 30-35 deg.C, pH is 7.0-8.0, and dissolved oxygen is 1-3.0 mg/L.
In order to improve the denitrification effect of denitrifying bacteria, the compositions prepared in Table 1 were added to the treatment system, and the compositions were numbered as examples 1 to 17 and comparative examples 1 to 6. Meanwhile, on the basis of the composition No. 1, a certain amount of ascorbic acid is added, wherein the addition amount is 0.5 part by weight and 1.5 parts by weight respectively, so that the compositions No. 24 and No. 25 are prepared, and the numbering is the examples 18 and 19.
The composition is added according to the concentration of 0.5mg/L in the sewage treatment system every day, after 10 days of adding, sampling is carried out to analyze the concentrations of COD, ammonia nitrogen, total nitrogen and nitrite nitrogen in the effluent, and the results are shown in Table 2.
TABLE 2 treatment effect with different formulation compositions
As seen from the data in Table 2, the initial COD was further reduced to less than 55mg/L after the use of the composition, the total nitrogen concentration of the effluent was less than 30mg/L, and the nitrite nitrogen concentration was less than 2 mg/L. In the absence of any of the composition formulations, the total nitrogen concentration of the effluent was above 40mg/L and the nitrite nitrogen concentration was above 8 mg/L.
Claims (13)
1. A composition for promoting denitrification by denitrifying microorganisms, comprising: the composition consists of glycolipid, organic acid salt and humic acid or/and humate, wherein the glycolipid is at least one of seaweed glycolipid, sophorolipid, rhamnolipid and fiber glycolipid; the organic acid salt is at least one of sodium acetate, sodium succinate and sodium citrate; the content of the glycolipid is 0.5 to 15 weight parts, the content of the organic acid salt is 0.5 to 15 weight parts, and the content of the humic acid or/and the humic acid salt is 5 to 30 weight parts.
2. The composition of claim 1, wherein: the sophorolipid is lactone-type sophorolipid or/and acid-type sophorolipid.
3. The composition of claim 2, wherein: the sophorolipid is lactone sophorolipid.
4. The composition of claim 1, wherein: the humate is at least one of sodium humate, potassium humate, magnesium humate and calcium humate.
5. The composition of claim 4, wherein: the humate is potassium humate.
6. The composition of claim 1, wherein: the organic acid salt is sodium succinate.
7. The composition of claim 1, 2, 3, 4, 5, or 6, wherein: the glycolipid content is 2-10 parts by weight; the organic acid salt is 2-10 weight parts; the content of humic acid or/and humate is 10-20 weight portions.
8. The composition of claim 1, wherein: the composition further comprises ascorbic acid in an amount of 1% -45% by weight of the glycolipid.
9. The composition of claim 8, wherein: the addition amount of ascorbic acid is 10% -30% of glycolipid weight.
10. A method of culturing denitrifying microorganisms, comprising: contacting a culture system comprising denitrifying microorganisms with a composition according to any one of claims 1 to 9.
11. A method for denitrification by denitrifying microorganisms, which is characterized by comprising the following steps: contacting a denitrification system comprising denitrifying microorganisms with the composition of any of claims 1-9.
12. The method according to claim 10 or 11, characterized in that: the dosage of the composition is 0.01-10 mg/L; the contact conditions are as follows: the temperature is 20-40 deg.C, pH is 6-9, and dissolved oxygen is 0.1-3 mg/L.
13. The method of claim 12, wherein: the dosage of the composition is 0.5-1.0 mg/L.
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Citations (4)
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| CN101928188A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Slow-release fertilizer with effect of soil remediation and remediation method of soil polluted by polycyclic aromatic hydrocarbons |
| CN102276065A (en) * | 2011-07-22 | 2011-12-14 | 大庆沃太斯化工有限公司 | Application of rhamnolipid biosurfactant in chemical sewage treatment |
| CN102586033A (en) * | 2011-01-13 | 2012-07-18 | 郑涵 | Biological remediation type water system oil stain cleaning agent and preparation method thereof |
| CN105624094A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Nitrifying bacterium culture promoter as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101928188A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Slow-release fertilizer with effect of soil remediation and remediation method of soil polluted by polycyclic aromatic hydrocarbons |
| CN102586033A (en) * | 2011-01-13 | 2012-07-18 | 郑涵 | Biological remediation type water system oil stain cleaning agent and preparation method thereof |
| CN102276065A (en) * | 2011-07-22 | 2011-12-14 | 大庆沃太斯化工有限公司 | Application of rhamnolipid biosurfactant in chemical sewage treatment |
| CN105624094A (en) * | 2014-10-28 | 2016-06-01 | 中国石油化工股份有限公司 | Nitrifying bacterium culture promoter as well as preparation method and application thereof |
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Effective date of registration: 20230913 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |