CN109880784A - It is a kind of promote denitrifying microorganism denitrogenation composition and its application - Google Patents
It is a kind of promote denitrifying microorganism denitrogenation composition and its application Download PDFInfo
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- CN109880784A CN109880784A CN201711279578.5A CN201711279578A CN109880784A CN 109880784 A CN109880784 A CN 109880784A CN 201711279578 A CN201711279578 A CN 201711279578A CN 109880784 A CN109880784 A CN 109880784A
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Abstract
The present invention relates to a kind of composition for promoting denitrifying microorganism denitrogenation and its applications, including glycolipid, acylate, humic acid or/and humate, wherein the glycolipid is at least one of seaweed glycolipid, sophorolipid, rhamnolipid, cellobiose rouge etc., preferably seaweed sugar ester.The composition formula is simple, and preparation is easy, and when being used for denitrification process, the denitrification activity of denitrifying microorganism can be improved, promote going on smoothly for denitrification denitrogenation process.
Description
Technical field
The invention belongs to field of biotechnology, and in particular to it is a kind of promote denitrifying microorganism denitrogenation composition and its answer
With.
Background technique
Denitrifying bacterium is a kind of amphimicrobe for nitrate nitrogen or nitrite nitrogen being converted into nitrogen.It is this kind of
Microorganism is generally only under conditions of anaerobism, nitrate reductase A and nitrous acid needed for just capable of inducing denitrification
Reductase carries out denitrogenation using nitrate or nitrite as electron acceptor.Since Institute of Micro-biology needs the difference of environment, in dirt
The entire denitrification process of water process, i.e. nitrifying process and denitrification process, the impact resistance of system is poor, the ammonia of high concentration
Nitrogen, nitrate nitrogen can inhibit the growth of nitrifier, and the dissolved oxygen and carbon source deficiency of high concentration can inhibit the growth of denitrifying bacterium, thus
Influence denitrogenation general effect.But currently without effective method for obtaining denitrifying bacterium, obtained thallus is used for sewage treatment
Carry out nitrification and denitrification process denitrogenation.
In recent years, in the aerobic denitrification pond in many actual motions, it is often found that there is the loss (Feng Yecheng of total nitrogen
Deng the experimental study of simultaneous nitrification-denitrification, Shanghai environmental science, 2002,21).Many researchs and report also confirm that both at home and abroad
The presence of aerobic denitrification (fourth loves medium, the experimental evidence [J] of aerobe anti-nitration reaction, Science Bulletin, 2000,
45).Therefore denitrogenation technology is developed using aerobic denitrifying bacteria, denitrification process can be made to carry out in the same reactor, significantly
Reduce occupied area and construction investment；The chemical substance of regulating system pH and the dosage of carbon source can be reduced, medicament expense is saved,
Reduce operating cost；COD(COD can also be removed while denitrogenation), enhance system impact resistance, treatment effect
It is good.
CN1800053A discloses a kind of method for carrying out nitrite denitrification using granule sludge, although having in system
The advantages that sludge concentration is high, reactor efficiency is high, simple process, but denitrification granular sludge needs are trained under the conditions of 15-30 DEG C
It could be obtained after supporting 30-90 days, the cultured water concentration in handled waste water is 10-200mg/L.CN101898828A is disclosed
A kind of cultural method of granular sludge by shortcut denitrification is by selecting different culture substrates that three phases is divided to realize denitrification
The enrichment culture of granule sludge.CN105621611A disclose it is a kind of using nitrite carry out denitrifying denitrogenation microbial inoculum and its
Using the invention mainly uses the bacterial strain of specific combination and proportion to realize denitrification denitrogenation, is related to a variety of bacterial strains, prepared
Journey is more complex.CN106754450A describes a kind of denitrifying microorganism culture promoter, mainly includes metal salt, more amines
Matter, organic acid azanol and acylate can promote the growth of denitrifying microorganism, improve treatment effect, but in promotor
Metal salt content is higher, will cause secondary pollution of water after adding for a long time.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides it is a kind of promote denitrifying microorganism denitrogenation composition and its answer
With.The composition formula is simple, and preparation is easy, and when being used for denitrification process, the denitrification activity of denitrifying microorganism can be improved,
Promote going on smoothly for denitrification denitrogenation process.
It is provided by the invention promote denitrifying microorganism denitrogenation composition, including glycolipid, acylate, humic acid or/
And humate, wherein the glycolipid is at least one of seaweed glycolipid, sophorolipid, rhamnolipid, cellobiose rouge etc., it is excellent
Select seaweed sugar ester.
The sophorolipid is lactone type and two kinds of acid type, preferably lactone type sophorolipid.
The humate is at least one of sodium humate, humic acid potassium, humic acid magnesium, calcium humate etc., excellent
Select humic acid potassium.
The acylate is at least one of acylates such as sodium acetate, sodium succinate and sodium citrate, preferably
Sodium succinate.
The glycolipid content is 0.5-15 parts by weight, preferably 2-10 parts by weight；Acylate is 0.5-15 parts by weight, preferably
For 2-10 parts by weight；Humic acid or/and humate content are 5-30 parts by weight, preferably 10-20 parts by weight.
Further, the composition further includes ascorbic acid, and additional amount is the 1%-45%, preferably 10%- of glycolipid weight
30%。
The present invention also provides the culture medium that can promote denitrifying microorganism denitrogenation, the culture medium contain nitrate and/
Or nitrite and above-mentioned composition.
The present invention also provides the methods of culture denitrifying microorganism, including that will contain the cultivating system of denitrifying microorganism
It is contacted with composition, or denitrifying microorganism is seeded in above-mentioned culture medium and is cultivated.
The present invention also provides the methods for carrying out denitrogenation using denitrifying microorganism, including making containing denitrifying microorganism
Denitrogenation system is contacted with composition.
In cultural method or denitrogenation method provided by the invention, the dosage of the composition is 0.01-10mg/L, preferably
0.5-1.0mg/L.The condition of the contact are as follows: temperature is 20-40 DEG C, pH 6-9, dissolved oxygen 0.1-3mg/L.
Through the above technical solutions, the growth of denitrifying microorganism can be promoted, to improve the de- of denitrifying microorganism
Nitrogen activity, promotes going on smoothly for denitrification denitrogenation process, and shortens the processing time.It is cultivated using composition of the invention
Denitrifying microorganism activity is high, flocculability is good, strong using time length, tolerance impact capacity.Moreover, composite formula of the invention
Simply, preparation is easy, and environmental protection and economy will not cause secondary pollution to water body.
Specific embodiment
Preparation method and effect of the invention are further illustrated below by embodiment.Embodiment is with the technology of the present invention
Implemented under premised on scheme, the detailed implementation method and specific operation process are given, but protection scope of the present invention
It is not limited to the following embodiments.
Experimental method in following embodiment is unless otherwise specified conventional method in that art.Institute in following embodiments
Experimental material is commercially available from routine biochemistry reagent shop unless otherwise specified.
In the present invention, the condition of the contact are as follows: temperature is 20-40 DEG C, pH 6-9, dissolved oxygen 0.1-3mg/L.Its
In, dissolved oxygen is measured by iodimetric titration.
In the present invention, the denitrifying microorganism can be (usually thin for the common various denitrifying microorganisms in this field
Bacterium), it is such as derived from certain sewage farm denitrification processing pond, total nitrogen removal efficiency is 80% or more.Denitrifying microorganism is with nitrate
And/or nitrite carries out respiration (respiration) as electron acceptor to obtain energy, thus realize denitrogenation, because
This, the nitrate and/or nitrite common can provide the inorganic nitrogen of electron acceptor to be various for denitrifying microorganism
Source.
In the present invention, the present invention will be described in detail by way of examples below.In following embodiment, COD concentration is adopted
It is measured with GB11914-89 " water quality-COD measurement-dichromate titration "；Ammonia nitrogen concentration uses GB7478-87 " water
Matter-ammonium measurement-distillation and titration " measurement；Nitrite nitrogen concentration uses GB7493-87 " water quality-nitrite nitrogen survey
Fixed-spectrophotometry " measurement；" water quality-total nitrogen measurement-alkaline chitinase resolution is purple using GB11894-89 for total nitrogen concentration
Outer spectrophotometry ".
According to the composition and ratio compositions formulated 1# to 24# of table 1.
The formula and ratio of 1 composition of table
In waste water caused by certain enterprise COD concentration be 450mg/L, ammonia nitrogen concentration 150mg/L, by nitration denitrification technique
After processing, COD concentration average out to 90mg/L, ammonia nitrogen concentration (NH in water outlet3- N) up to lower than 15mg/L, total nitrogen concentration (TN)
70mg/L, Nitrite Nitrogen (NO2 -N) concentration is 40mg/L.Temperature is 30-35 DEG C, pH 7.0-8.0 in treatment process,
Dissolved oxygen is 1-3.0mg/L.
In order to improve the denitrification effect of denitrifying bacterium, the composition of the preparation of table 1 is added in the processing system, by composition sequence
Number number is embodiment 1- embodiment 17, comparative example 1-6.Simultaneously on the basis of 1# composition, a certain amount of ascorbic acid is added,
Additional amount is respectively 0.5 parts by weight, 1.5 parts by weight, and preparation obtains composition 24#, 25#, and number is embodiment 18,19.
It is daily that 0.5mg/L is added according to composition concentration in sewage treatment system, after adding 10 days, sampling analysis
It is discharged COD, ammonia nitrogen, total nitrogen, nitrite nitrogen concentration, the results are shown in Table 2.
Table 2 uses the treatment effect of different formulations composition
From the point of view of the data of table 2, is further decreased using COD initial after composition to less than 55mg/L, water outlet total nitrogen concentration are low
It is lower than 2mg/L in 30mg/L, nitrite nitrogen concentration.When lacking any one of composite formula, it is discharged total nitrogen concentration
It is above 40mg/L, nitrite nitrogen concentration is above 8mg/L.
Claims (10)
1. it is a kind of promote denitrifying microorganism denitrogenation composition, it is characterised in that: the composition include glycolipid, acylate,
Humic acid or/and humate, wherein the glycolipid be in seaweed glycolipid, sophorolipid, rhamnolipid, cellobiose rouge etc. extremely
Few one kind.
2. composition according to claim 1, it is characterised in that: the sophorolipid is lactone type sophorolipid or/and acid type
Sophorolipid, preferably lactone type sophorolipid.
3. composition according to claim 1, it is characterised in that: the humate is sodium humate, humic acid potassium, corruption
At least one of phytic acid magnesium, calcium humate etc., preferably humic acid potassium.
4. composition according to claim 1, it is characterised in that: the acylate is sodium acetate, sodium succinate and lemon
At least one of lemon acid sodium acylate, preferably sodium succinate.
5. composition according to claim 1,2,3 or 4, it is characterised in that: the glycolipid content is 0.5-15 parts by weight,
It is preferred that 2-10 parts by weight；Acylate is 0.5-15 parts by weight, preferably 2-10 parts by weight；Humic acid or/and humate content
For 5-30 parts by weight, preferably 10-20 parts by weight.
6. composition according to claim 1, it is characterised in that: the composition further includes ascorbic acid, and additional amount is
The 1%-45% of glycolipid weight, preferably 10%-30%.
7. the culture medium that one kind can promote denitrifying microorganism denitrogenation, it is characterised in that: the culture medium contain nitrate and/or
Nitrite and composition as claimed in any one of claims 1 to 6.
8. a kind of method for cultivating denitrifying microorganism, it is characterised in that: by cultivating system and power containing denitrifying microorganism
Benefit requires composition described in 1-6 any one to contact, or denitrifying microorganism is seeded in culture medium described in claim 7
It is cultivated.
9. a kind of method for carrying out denitrogenation using denitrifying microorganism, it is characterised in that: make the denitrogenation containing denitrifying microorganism
System is contacted with composition described in claim 1-6 any one.
10. method according to claim 8 or claim 9, it is characterised in that: the dosage of the composition is 0.01-10mg/L, excellent
It is selected as 0.5-1.0mg/L；The condition of the contact are as follows: temperature is 20-40 DEG C, pH 6-9, dissolved oxygen 0.1-3mg/L.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110697887A (en) * | 2019-09-03 | 2020-01-17 | 天津大学 | Method for domesticating humic acid reducing bacteria based on humic acid reduction-denitrification coupling |
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| CN102276065A (en) * | 2011-07-22 | 2011-12-14 | 大庆沃太斯化工有限公司 | Application of rhamnolipid biosurfactant in chemical sewage treatment |
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2017
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| CN101928188A (en) * | 2009-06-19 | 2010-12-29 | 深圳市意可曼生物科技有限公司 | Slow-release fertilizer with effect of soil remediation and remediation method of soil polluted by polycyclic aromatic hydrocarbons |
| CN102586033A (en) * | 2011-01-13 | 2012-07-18 | 郑涵 | Biological remediation type water system oil stain cleaning agent and preparation method thereof |
| CN102276065A (en) * | 2011-07-22 | 2011-12-14 | 大庆沃太斯化工有限公司 | Application of rhamnolipid biosurfactant in chemical sewage treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110697887A (en) * | 2019-09-03 | 2020-01-17 | 天津大学 | Method for domesticating humic acid reducing bacteria based on humic acid reduction-denitrification coupling |
| CN110697887B (en) * | 2019-09-03 | 2022-05-13 | 天津大学 | Method for domesticating humic acid reducing bacteria based on humic acid reduction-denitrification coupling |
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Effective date of registration: 20230913 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |
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