CN105543158A - Nitrifying bacterial growth promoting agent, preparation method and applications thereof - Google Patents
Nitrifying bacterial growth promoting agent, preparation method and applications thereof Download PDFInfo
- Publication number
- CN105543158A CN105543158A CN201410585482.1A CN201410585482A CN105543158A CN 105543158 A CN105543158 A CN 105543158A CN 201410585482 A CN201410585482 A CN 201410585482A CN 105543158 A CN105543158 A CN 105543158A
- Authority
- CN
- China
- Prior art keywords
- salt
- metal
- weight parts
- growth stimulant
- nitrobacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a nitrifying bacterial growth promoting agent, which comprises, by weight, 40-100 parts of a metal salt and 5-30 parts of a polyamine substance, preferably 50-80 parts of a metal salt and 10-20 parts of a polyamine substance, wherein the metal salt is a calcium salt, a magnesium salt, a ferrous salt and a copper salt, and a molar ratio of Ca<2+> to Mg<2+> to Fe<2+> to Cu<2+> is (5-15):(5-25):(1-8):(0.5-5), preferably (8-12):(10-20):(2-6):(1-4). According to the present invention, the formula of the nitrifying bacterial growth promoting agent is simple, the preparation is easy, the nitrifying bacterial growth promoting agent can be used for the nitrifying bacterial culture process and can further be directly poured into the sewage treatment system so as to make the nitrifying bacteria rapidly grow and breed within a short time, and the culture nitrifying bacteria have characteristics of high activity and strong impact resistance.
Description
Technical field
The invention belongs to biological technical field, be specifically related to a kind of nitrobacteria growth stimulant and its preparation method and application.
Background technology
In biological denitrification method, no matter be traditional nitrification-denitrification, or novel short-cut nitrification-denitrification and short distance nitration-anaerobic ammoxidation all need the nitrification removal of ammonia and nitrogen through nitrobacteria.Nitrobacteria belongs to chemotrophy type microorganism.Biomass cells can only utilize the energy preserved with forms such as ATP, the free energy that can not directly utilize chemical reaction to discharge.In good oxygen metabolism, ATP synthesizes mainly through the oxidative phosphorylation of respiratory chain.Ammonia oxidation phosphorylation efficiency is very low, and the ATP that can produce is very limited, and these energy are mainly used in transition of electron to higher energy level, and it is very slow that this makes nitrobacteria grow, and the phase is 8 ~ 36h from generation to generation.In the cell walls of nitrobacteria, peptidoglycan content is low, protein and lipid content high, therefore more responsive to environmental change, the nitrobacteria adaptability that occurring in nature is natural and tolerance poor, cannot get the mastery in growth competition with heterotroph microorganism under a lot of condition.In Sewage treatment systems, when in active sludge, nitrobacteria content is lower, rely on regulate the envrionment conditions such as dissolved oxygen and pH cannot within a short period of time quick growth and breeding, finally cause the Sewage treatment systems removal of ammonia and nitrogen of existing operation limited in one's ability, the cultured high density nitrobacteria of direct input in Sewage treatment systems industrially usually can be adopted to solve this problem.No matter be directly in Sewage treatment systems, cultivate nitrobacteria or cultivate nitrobacteria outward in Sewage treatment systems, all need the material adding growth promoting effects, the growth velocity of nitrobacteria could be accelerated.
From biochemistry level, nitration reaction relates to the pathways metabolism of ammona monooxygenase, azanol oxygen also enzyme and nitrite oxidase co-catalysis, and transforms along with the matter and energy of complexity.Adopt Biostimulation agent to improve the activity of these enzymes in nitrobacteria, be then solve nitrobacteria to grow one of slow effective way, nitration reaction process can be accelerated simultaneously.A lot of about the research of Biostimulation agent at present, CN200510111874.5, CN200510111876.4, CN200510111877.9 and CN200510111875.X propose the nitrobacteria growth promoter utilizing different metal-salts to combine respectively, and main component comprises molasses, metal-salt (ferrous salt, manganese salt, calcium salt and magnesium salts) and sorbent material.After using this promotor, ammonia nitrogen removal frank can improve more than 20%.But due to the sorbent material mainly material such as zeolite powder, diatomite, Powdered Activated Carbon or flyash, adding of these sorbent materials will certainly increase sludge yield.CN201110315549.6 discloses a kind of cultural method of short-cut nitrification and denitrification granular sludge, it is characterized in that regularly adding 5 ~ 15mg/L azanol; CN201010168453.7 discloses a kind of method of quickly starting oxygen-poor ammonia oxidizing biofilter, it is characterized in that adding azanol in step (2), challenge inoculation mud changes to oxygen-poor ammonia oxidizing microbial film, more than invent the restraining effect mainly utilizing azanol to NOB, promote short distance nitration effect.CN201410141638.7 discloses a kind of nutrient solution of nitrite bacteria and preparation and cultural method, its nutrient solution formula more complicated, the element of Growth of Cells being provided except relating to iron, calcium, potassium, magnesium etc., also relating to the element that cobalt, molybdenum, manganese, zinc etc. promote enzymic synthesis.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of nitrobacteria growth stimulant and its preparation method and application.This promotor formula is simple, preparation easily, may be used in the culturing process of nitrobacteria, also can directly be added in Sewage treatment systems, make nitrobacteria can quick growth and breeding at short notice, the nitrobacteria activity of cultivating be high, withstand shock ability is strong.
Nitrobacteria growth stimulant of the present invention, comprises metal-salt and polyamines, and with parts by weight, metal-salt is 40 ~ 100 weight parts, and be preferably 50 ~ 80 weight parts, polyamines is 5 ~ 30 weight parts, is preferably 10 ~ 20 weight parts; Described metal-salt is calcium salt, magnesium salts, ferrous salt and mantoquita, Ca
2+, Mg
2+, Fe
2+and Cu
2+mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (0.5 ~ 5), be preferably (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4).
Calcium salt of the present invention is CaSO
4or CaCl
2, preferred CaSO
4; Magnesium salts is MgSO
4or MgCl
2, preferred MgSO
4; Ferrous salt is FeSO
4or FeCl
2, preferred FeSO
4; Mantoquita is CuSO
4or CuCl
2, preferred CuSO
4.
Polyamines of the present invention is spermine, spermidine or both mixtures.
Promotor of the present invention can also comprise mineral acid azanol, and content is 0.5 ~ 15 weight part, is preferably 2 ~ 10 weight parts.Described mineral acid azanol is one or more in oxammonium hydrochloride, oxammonium sulfate or phosphatic hydroxylamine, is preferably oxammonium sulfate.
The preparation method of nitrobacteria growth stimulant of the present invention, comprise following content: (1) prepares metal salt solution according to following composition and weight part: metal-salt is 40 ~ 100 weight parts, be preferably 50 ~ 80 weight parts, described metal-salt is calcium salt, ferrous salt and mantoquita, Ca in metal-salt
2+, Mg
2+, Fe
2+and Cu
2+mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (1 ~ 4), be preferably (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4); (2) by 5 ~ 30 weight parts before using, the polyamines being preferably 10 ~ 20 weight parts joins in metal salt solution.
Calcium salt described in step of the present invention (1) is CaSO
4or CaCl
2, preferred CaSO
4; Magnesium salts is MgSO
4or MgCl
2, preferred MgSO
4; Ferrous salt is FeSO
4or FeCl
2, preferred FeSO
4; Mantoquita is CuSO
4or CuCl
2, preferred CuSO
4.
Polyamines described in step of the present invention (2) is spermine, spermidine or both mixtures.
Further, also adding content before using in step of the present invention (2) is 0.5 ~ 15 weight part, be preferably the mineral acid azanol of 2 ~ 10 weight parts, described mineral acid azanol is one or more in oxammonium hydrochloride, oxammonium sulfate or phosphatic hydroxylamine, is preferably oxammonium sulfate.
Nitrobacteria growth stimulant of the present invention may be used for the culturing process of nitrobacteria, may be used for the ammonia nitrogen removal effect improving Sewage treatment systems, can also be used for containing the quick startup of ammonia system and the fast quick-recovery of the system that is hit.Concrete needs are according to sewage character, treatment capacity and treatment effect determination dosage.In use, first promotor is dissolved, then add according to promoter concentration 10 ~ 100mg/L in sewage.
Compared with prior art, the present invention has following beneficial effect:
1, the metal ion utilizing metal-salt to provide, as the element required for nitrobacteria growth, improves the activity of enzyme simultaneously as the component of enzyme, can fast degradation substrate, accelerates enzymatic reaction process.Polyamines and metal ion acting in conjunction, can accelerate cell proliferation, and can improve settleability and the stability of gathered in the crops thalline, extends thalline work-ing life.
2, mineral acid azanol add in right amount can as the matrix of azanol oxygen also enzyme participate in directly nitrobacteria metabolic process, shorten enzymatic reaction process, simultaneously can accelerated cell growth as the activator of cell.
3, the present invention is by selecting component and the proportioning of nitrobacteria growth stimulant, under the acting in conjunction of metal-salt, polyamines and mineral acid azanol, realizes the object of fast culture nitrobacteria.The invention solves nitrobacteria and grow slow problem, nitration reaction process can also be accelerated simultaneously, improve the treatment effect of waste water.After using promotor, the growth velocity of nitrobacteria can increase by 10 ~ 100 times, and the ammonia nitrogen removal frank of ammonia-containing water can improve more than 30%, can also solve the fast recovery problem of the nitrated ammonia nitrogen removal ability of the Sewage treatment systems be hit well.
Embodiment
Below in conjunction with specific embodiment, the present invention program is described in detail.
The formula of the nitrobacteria growth stimulant of embodiment 1-8 and comparative example 1-4 is in table 1.
The formula of table 1 promotor and ratio
The principal pollutant COD concentration contained in the waste water of certain enterprise discharge is about 800mg/L, ammonia nitrogen concentration is about 200mg/L, this factory adopts batch activated sludge process (SBR technique) to process, but in water outlet after treatment, COD concentration is for being less than 60mg/L ammonia nitrogen concentration still up to more than 60mg/L.Utilization is shown the promotor that proportioning configures, configuration concentration is 0.5g/L, every day is that 20mg/L adds according to promoter concentration in sewage, after adding 15 days, ammonia nitrogen removal frank improves more than 10% to be stopped adding, system continues operation 10 days, and water sampling analyzes water suspension concentration and water outlet ammonia nitrogen concentration and calculates ammonia nitrogen removal frank.Add rear concrete result in table 2.
Table 2 adopts the treatment effect of different ingredients promotor
From the effluent quality of each embodiment of table 2, when adding promotor, water outlet concentration of suspension is 150mg/L, water outlet ammonia nitrogen concentration is 80mg/L, ammonia nitrogen removal frank only has 60%, after adding promotor, water outlet concentration of suspension is all lower than 100mg/L, water outlet ammonia nitrogen concentration is all lower than 15mg/L, ammonia nitrogen removal frank is all greater than 90%, when promotor is only containing the metal-salt in formula or polyamines, water outlet concentration of suspension between 120 ~ 140mg/L, water outlet ammonia nitrogen concentration still between 50 ~ 70mg/L, ammonia nitrogen removal frank is lower than 80%.As can be seen here, growth stimulant of the present invention is used can to significantly improve the clearance of ammonia nitrogen in dirt.In addition, the flocculating property of mud can also be improved, reduce water outlet concentration of suspension.
Claims (11)
1. a nitrobacteria growth stimulant, is characterized in that comprising metal-salt and polyamines, and with parts by weight, metal-salt is 40 ~ 100 weight parts, and polyamines is 5 ~ 30 weight parts; Described metal-salt is calcium salt, magnesium salts, ferrous salt and mantoquita, Ca
2+, Mg
2+, Fe
2+and Cu
2+mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (0.5 ~ 5).
2. according to growth stimulant according to claim 1, it is characterized in that: metal-salt is 50 ~ 80 weight parts, polyamines is 10 ~ 20 weight parts, Ca
2+, Mg
2+, Fe
2+and Cu
2+mol ratio be (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4).
3. according to the growth stimulant described in claim 1 or 2, it is characterized in that: calcium salt is CaSO
4or CaCl
2, magnesium salts is MgSO
4or MgCl
2, ferrous salt is FeSO
4or FeCl
2, mantoquita is CuSO
4or CuCl
2.
4. according to the growth stimulant described in claim 1 or 2, it is characterized in that: calcium salt is CaSO
4, magnesium salts is MgSO
4, ferrous salt is FeSO
4, mantoquita is CuSO
4.
5. according to the growth stimulant described in claim 1 or 2, it is characterized in that: polyamines is spermine, spermidine or both mixtures.
6. according to the growth stimulant described in claim 1 or 2, it is characterized in that: described promotor can also comprise mineral acid azanol, content is 0.5 ~ 15 weight part.
7. according to growth stimulant according to claim 6, it is characterized in that: mineral acid azanol is one or more in oxammonium hydrochloride, oxammonium sulfate or phosphatic hydroxylamine, content is 2 ~ 10 weight parts.
8. according to growth stimulant according to claim 6, it is characterized in that: described mineral acid azanol is oxammonium sulfate.
9. the preparation method of a nitrobacteria growth stimulant, it is characterized in that comprising following content: (1) prepares metal salt solution according to following composition and weight part: metal-salt is 40 ~ 100 weight parts, be preferably 50 ~ 80 weight parts, described metal-salt is calcium salt, magnesium salts, ferrous salt and mantoquita, Ca in metal-salt
2+, Mg
2+, Fe
2+and Cu
2+mol ratio be (5 ~ 15): (5 ~ 25): (1 ~ 8): (0.5 ~ 5), be preferably (8 ~ 12): (10 ~ 20): (2 ~ 6): (1 ~ 4); (2) by 5 ~ 30 weight parts before using, the polyamines being preferably 10 ~ 20 weight parts joins in metal salt solution.
10. in accordance with the method for claim 9, it is characterized in that: before using in step (2), the mineral acid azanol that content is 0.5 ~ 15 weight part can also be added, be preferably 2 ~ 10 weight parts; Described mineral acid azanol is one or more in oxammonium hydrochloride, oxammonium sulfate or phosphatic hydroxylamine.
The application of the arbitrary described growth stimulant of 11. claim 1-10, is characterized in that: in use, first promotor is dissolved, then add according to promoter concentration 10 ~ 100mg/L in sewage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410585482.1A CN105543158B (en) | 2014-10-28 | 2014-10-28 | Nitrobacteria growth promoter and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410585482.1A CN105543158B (en) | 2014-10-28 | 2014-10-28 | Nitrobacteria growth promoter and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105543158A true CN105543158A (en) | 2016-05-04 |
| CN105543158B CN105543158B (en) | 2020-02-14 |
Family
ID=55822716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410585482.1A Active CN105543158B (en) | 2014-10-28 | 2014-10-28 | Nitrobacteria growth promoter and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105543158B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117158A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | A kind of fast culture process of nitrogen removal granule sludge |
| CN111807507A (en) * | 2020-06-05 | 2020-10-23 | 广东工业大学 | Method for promoting activity of nitrifying bacteria |
| CN114804349A (en) * | 2022-04-24 | 2022-07-29 | 桂林电子科技大学 | Anaerobic ammonium oxidation sludge activator and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101899401A (en) * | 2009-05-25 | 2010-12-01 | 中国石油化工股份有限公司 | Microbial agent for treating ammonia-containing waste water and preparation method thereof |
-
2014
- 2014-10-28 CN CN201410585482.1A patent/CN105543158B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101899401A (en) * | 2009-05-25 | 2010-12-01 | 中国石油化工股份有限公司 | Microbial agent for treating ammonia-containing waste water and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| GUANGJING XU,ET AL: "Partial nitrification adjusted by hydroxylamine in aerobic granules under high DO and ambient temperature and subsequent Anammox for low C/N wastewater treatment", 《；CHEMICAL ENGINEERING JOURNAL 》 * |
| SCOTT A. ENSIGN, ET AL: "In Vitro Activation of Ammonia Monooxygenase from Nitrosomonas europaea by Copper", 《JOURNAL OF BACTERIOLOGY》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108117158A (en) * | 2016-11-29 | 2018-06-05 | 中国石油化工股份有限公司 | A kind of fast culture process of nitrogen removal granule sludge |
| CN108117158B (en) * | 2016-11-29 | 2020-09-11 | 中国石油化工股份有限公司 | Rapid culture method of denitrified granular sludge |
| CN111807507A (en) * | 2020-06-05 | 2020-10-23 | 广东工业大学 | Method for promoting activity of nitrifying bacteria |
| CN114804349A (en) * | 2022-04-24 | 2022-07-29 | 桂林电子科技大学 | Anaerobic ammonium oxidation sludge activator and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105543158B (en) | 2020-02-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Efficient ammonia removal in recirculating vertical flow constructed wetlands: complementary roles of anammox and denitrification in simultaneous nitritation, anammox and denitrification process | |
| CN105624094B (en) | Nitrifying bacteria culture promoter and preparation method and application thereof | |
| CN106754450A (en) | A kind of denitrifying microorganism culture promoter and its preparation method and application | |
| CN105621611A (en) | Method for quickly starting short-cut nitrification and denitrification of ammonia-containing wastewater | |
| CN106434469B (en) | Low-temperature-resistant nitrifying bacteria agent and preparation method and application thereof | |
| CN105624093A (en) | Nitrite bacterium culture promoter and preparation method thereof | |
| CN105585133A (en) | Bio-denitrification method for high-salt-content wastewater discharged from catalyst production process | |
| CN105543158A (en) | Nitrifying bacterial growth promoting agent, preparation method and applications thereof | |
| CN105624086B (en) | Nitrobacteria growth promoter and preparation method thereof | |
| Zhou et al. | Enhancing nitrogen removal from anaerobically-digested swine wastewater through integration of Myriophyllum aquaticum and free nitrous acid-based technology in a constructed wetland | |
| CN106554076B (en) | Biological treatment method of ammonia-containing wastewater | |
| CN105624053A (en) | Enrichment culture method for nitrifying bacteria | |
| CN106745727B (en) | Denitrification method for ammonia-containing wastewater discharged in acrylic fiber production process | |
| CN105502651A (en) | Nutritional agent for organic silicon wastewater anaerobic biological treatment | |
| CN105621610B (en) | A kind of saline sewage simultaneous nitrification-denitrification denitrogenation method | |
| CN105624088B (en) | Ammonia oxidizing bacteria growth promoter and preparation method and application thereof | |
| CN105621624B (en) | A kind of denitrogenation method of acrylic fiber production process process discharge ammonia-containing water | |
| CN105621625B (en) | A kind of nitrite bacteria growth promoter and preparation method thereof | |
| CN105624090B (en) | Nitrifying bacteria culture promoter and preparation method thereof | |
| CN112707500A (en) | Method for preparing biofilter filler nutrient by using poultry manure | |
| CN106754616A (en) | A kind of cultural method of nitrosation dominant microflora | |
| CN105624054A (en) | Enrichment culture method for salt-tolerant nitrifying bacteria colonies | |
| CN105624095A (en) | Enrichment culture method for nitrifying bacteria by waste water generated in catalyst production process | |
| CN105624089A (en) | Promoter for culturing ammonia oxidizing bacteria and preparation method of promoter | |
| CN109880785B (en) | Composition capable of promoting denitrification of nitrifying bacteria and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230828 Address after: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee after: CHINA PETROLEUM & CHEMICAL Corp. Patentee after: Sinopec (Dalian) Petrochemical Research Institute Co.,Ltd. Address before: 100728 No. 22 North Main Street, Chaoyang District, Beijing, Chaoyangmen Patentee before: CHINA PETROLEUM & CHEMICAL Corp. Patentee before: DALIAN RESEARCH INSTITUTE OF PETROLEUM AND PETROCHEMICALS, SINOPEC Corp. |