CN108118021A - Complete microbial growth promoters and its application of nitrifying process - Google Patents
Complete microbial growth promoters and its application of nitrifying process Download PDFInfo
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- CN108118021A CN108118021A CN201611072922.9A CN201611072922A CN108118021A CN 108118021 A CN108118021 A CN 108118021A CN 201611072922 A CN201611072922 A CN 201611072922A CN 108118021 A CN108118021 A CN 108118021A
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- accelerating agent
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- sophorolipid
- glycolipid
- nitrobacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Activated Sludge Processes (AREA)
- Treatment Of Biological Wastes In General (AREA)
Abstract
The present invention relates to a kind of microbial growth promoters for completing nitrifying process, and including at least one of seaweed glycolipid, sophorolipid and rhamnolipid etc. glycolipid, preferably sophorolipid, wherein sophorolipid is divided to two kinds of lactone type and acid type, more preferable acid type sophorolipid.The present invention breathes out the application for providing the microbial growth promoters, can be used in the incubation of nitrobacteria, can also be added directly in the sewage disposal system including de-nitrification unit, for improving the ammonia nitrogen removal effect of sewage disposal system；It can be used for quickly starting containing ammonia system and the fast quick-recovery for the system that is hit.The promotion agent prescription is simple, and it is easy to prepare, during for nitrifying process, the features such as can making slow-growing nitrobacteria, fast-growth is bred in a short time, and the thalline cultivated is active high, flocculability is good, usage time is long, tolerance impact capacity is strong.
Description
Technical field
The invention belongs to microbial technology fields, and in particular to it is a kind of complete nitrifying process microbial growth promoters and
It is applied.
Background technology
In biological denitrification method, either traditional nitrification-denitrification or new short-cut nitrification-denitrification and short
Journey nitration-anaerobic ammoxidation all needs the nitrification removal of ammonia and nitrogen by nitrobacteria.It is micro- that nitrobacteria belongs to chemotrophy type
Biology, biological cell can only utilize the energy preserved in the form of ATP etc., it is impossible to directly utilize the discharged freedom of chemical reaction
Energy.In aerobic metabolism, ATP is mainly synthesized by the oxidative phosphorylation of respiratory chain.Ammoxidation phosphorylation efficiency is very low, institute
The ATP that can be generated is very limited, these energy are mainly used for electron transition to higher energy level, this so that nitrobacteria growth is very slow
Slowly, phase generation is 8-36h.Peptidoglycan content is low in the cell membrane of nitrobacteria, and protein and fat content are high, therefore to environment
Variation is more sensitive, and natural nitrobacteria adaptability and tolerance are poor in nature, under the conditions of many can not with it is different
Type microorganism is supported to get the mastery in growth competition.In sewage disposal system, when nitrobacteria content is relatively low in activated sludge,
It fast-growth can not be bred within a short period of time by the environmental conditions such as dissolved oxygen and pH are adjusted, ultimately result in the dirt of existing operation
Water treatment system removal of ammonia and nitrogen ability is limited, industrially usually may be employed directly to launch into sewage disposal system and cultivate
High concentration nitrobacteria solve the problems, such as this.Nitrobacteria is cultivated either directly in sewage disposal system still in dirt
Nitrobacteria is cultivated outside water treatment system, all very slowly, cultivation cycle is longer for its growth.From biochemistry level, nitration reaction
It is the metabolic pathway for being related to ammona monooxygenase, azanol oxygen also enzyme and nitrite oxidase co-catalysis, and with complexity
Matter and energy converts.The activity of these enzymes in nitrobacteria is improved using Biostimulation agent, then is to solve nitrobacteria life
One of long slow effective way, while nitration reaction process can be accelerated.
There are many research at present on Biostimulation agent, Chinese patent CN200510111874.5,
CN200510111876.4, CN200510111877.9 and CN200510111875.X are proposed respectively utilizes different metal salts
The nitrobacteria growth promoter being composed, main component include molasses, metal salt（Molysite, manganese salt, calcium salt and magnesium salts）And suction
Attached dose.More than 20% can be improved using ammonia nitrogen removal frank after the accelerating agent.But due to adsorbent be mainly zeolite powder, diatomite,
The substances such as Powdered Activated Carbon or flyash, adding for these adsorbents will certainly increase sludge yield.CN200680027611.9
It discloses a kind of growth promoter and prolongs life agent, using sugar alcohol as active ingredient, be mainly used for letting animals feed in terms of agricultural, training
Support microorganism, cultivated plant or mushroom growth promoter and prolong life agent, when giving the sugar alcohols such as animal, plant, microorganism, mushroom
Particularly erythrite when, there is growth-promoting effect, cultivation effect, survival rate improvement effect, life prolongation effect, repairing effect etc..
The invention is not directed to the microculture of field of waste water treatment, even if suitable for denitrogenation of waste water field, due to its broad spectrum activity, is promoting
Into the growth that can also promote other microorganisms and miscellaneous bacteria while nitrobacteria growth, the specificity for promoting nitrobacteria growth is bad.
Chinese patent CN201410585418.3, CN201410585417.9, CN201410585482.1 and
Nitrobacteria accelerating agent disclosed in CN201410585430.4 etc. mainly includes metal salt and polyamines, can promote
The growth of nitrifying microorganisms improves treatment effect.But metal salt concentration is higher in accelerating agent, and sewage can be caused after adding for a long time
Secondary pollution.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides it is a kind of complete nitrifying process microbial growth promoters and its
Using.The promotion agent prescription is simple, and it is easy to prepare, and during for nitrifying process, can make slow-growing nitrobacteria in short-term
Interior fast-growth breeding, the active height of thalline cultivated, flocculability is good, usage time is long, it is strong etc. to be resistant to impact capacity
Feature.
The present invention completes the microbial growth promoters of nitrifying process, including seaweed glycolipid, sophorolipid and rhamnolipid etc.
At least one of glycolipid, preferably sophorolipid, wherein sophorolipid be divided to two kinds of lactone type and acid type, more preferable acid type sophorolipid.
Further, the accelerating agent further includes sugar alcohol, and the sugar alcohol is selected from mannitol, xylitol, lactitol, ribose
One or more of alcohol, galactitol, inositol and erythrite etc., preferably lactitol.
In accelerating agent of the present invention, the amount of sugar alcohol is that the mass ratio of glycolipid and sugar alcohol is made to be 1:1-1:5.
The application of microbial growth promoters of the present invention can be used in the incubation of nitrobacteria, can also
It is added directly in the sewage disposal system including de-nitrification unit, for improving the ammonia nitrogen removal effect of sewage disposal system；
It can be used for quickly starting containing ammonia system and the fast quick-recovery for the system that is hit.It is specific to need according to sewage property, treating capacity
Dosage and occupation mode are determined with treatment effect.In use, accelerating agent is dissolved first, then daily according to sewage
Middle promoter concentration 0.01-1mg/L, preferably 0.05-0.5mg/L are added.20-40 DEG C of temperature during control process, pH
For 6.0-9.0, dissolved oxygen 1.0-5.0mg/L.
The application of microbial growth promoters of the present invention adds a certain amount of auxiliary agent simultaneously in use, helps
Agent can be one or both of polyamines, inorganic acid azanol, preferably add two kinds of substances simultaneously.Wherein more amines
Matter is the mixture of spermine, spermidine or both, and inorganic acid azanol is in hydroxylamine hydrochloride, hydroxyl sulfate or phosphatic hydroxylamine etc.
It is one or more of.The dosage of the auxiliary agent is 0.1-0.5 times of promoter concentration.The cooperation of auxiliary agent adds in, and is bacterial growth
Element needed for offer, while the component as enzyme improves the activity of enzyme, the matrix as azanol oxygen also enzyme directly participates in bacterium
Metabolic process shortens enzymatic reaction process, further improves thalline performance with glycolipid, sugar alcohol synergistic effect and sewage disposal is imitated
Fruit.
The growth promoter of the present invention under the collective effect of glycolipid, sugar alcohol etc., can be realized using glycolipid as key component
The purpose of fast culture nitrifying microorganisms.The nitrifying microorganisms activity cultivated is high, flocculability is good, usage time is long, tolerance punching
Hit that ability is strong, tolerance salt content is high.During for biochemical treatment, be conducive to play the population effect of microorganism, improve microorganism pair
The resilience with high salt for waiting poor environments improves the ability of degradation of contaminant.
Glycolipid uses sophorolipid in accelerating agent of the present invention, and sophorolipid has good environment and bio-compatibility, that is, has
Nontoxic, harmless, degradable characteristic can increase the contact area of microorganism and matrix, improve mass-transfer efficiency, and then improve life
The growth rate and bioactivity of object, will not cause secondary pollution.
The present invention solves the problems, such as that nitrobacteria growth is slow, while may also speed up nitration reaction process, improves waste water
Treatment effect.10-100 times can be increased using the growth rate of nitrobacteria after accelerating agent, the ammonia nitrogen removal of ammonia-containing water
Rate can improve more than 30%, moreover it is possible to well solve the fast quick-recovery for the sewage disposal system nitrification ammonia nitrogen removal ability being hit
Problem.
Specific embodiment
The present invention program and effect are described in detail with reference to specific embodiment, but protection scope of the present invention is simultaneously
The scope of embodiment description is not limited only to, embodiment is only to facilitate more fully understand the present invention.
The formula for the microbial growth promoters that the embodiment of the present invention uses specifically is shown in Table 1.
The composition and ratio of 1 accelerating agent of table
Embodiment 1
The major pollutants COD concentration contained in the waste water of certain plant emissions is 400mg/L or so, ammonia nitrogen concentration 150mg/L
Left and right, the factory use batch activated sludge process（SBR techniques）Handled, in water outlet after treatment COD concentration be less than
60mg/L but ammonia nitrogen concentration are still up to more than 50mg/L.Add in the processing system table 1 preparation accelerating agent, daily according to
Promoter concentration is added for 0.5mg/L in sewage, and after adding 15 days, ammonia nitrogen removal frank improves more than 10%, stops throwing
Add, system continues to run with 10 days, and water sampling analysis water outlet ammonia nitrogen concentration simultaneously calculates ammonia nitrogen removal frank, specific treatment effect such as table 2
It is shown.
Table 2 uses the treatment effect of different accelerating agents
As seen from the data in Table 2, using water outlet ammonia nitrogen concentration after accelerating agent less than 15mg/L, ammonia nitrogen removal frank is more than 70%.The present invention
Growth promoter can significantly improve the removal effect of ammonia nitrogen in sewage.
Embodiment 2
Treatment process and operating condition with embodiment 1, the difference is that：A certain amount of help is added while adding 1# accelerating agents
Agent, the composition of auxiliary agent, dosage and effect are as shown in table 3.
Table 3 adds the treatment effect of different auxiliary agents simultaneously
As shown in Table 3, the cooperation of auxiliary agent adds in, and ammonia nitrogen concentration is further reduced to less than 10mg/L, and removal rate is more than 80%, into
One step improves treatment effect.
Claims (10)
1. a kind of microbial growth promoters for completing nitrifying process, it is characterised in that including seaweed glycolipid, sophorolipid and sandlwood
At least one of glycolipid glycolipid.
2. accelerating agent according to claim 1, it is characterised in that：The accelerating agent includes acid type sophorolipid.
3. accelerating agent according to claim 1 or 2, it is characterised in that：The accelerating agent further includes sugar alcohol, the sugar alcohol choosing
From one or more of mannitol, xylitol, lactitol, ribitol, galactitol, inositol and erythrite etc..
4. accelerating agent according to claim 3, it is characterised in that：The amount of sugar alcohol is that the mass ratio of glycolipid and sugar alcohol is made to be 1:
1-1:5。
5. the application of microbial growth promoters described in Claims 1-4, it is characterised in that：The accelerating agent is used for nitrobacteria
Incubation in or be added directly in the sewage disposal system including de-nitrification unit, for improving sewage disposal system
Ammonia nitrogen removal effect；Or for quickly starting containing ammonia system and the fast quick-recovery for the system that is hit.
6. the application of accelerating agent according to claim 5, it is characterised in that：In use, accelerating agent is dissolved first,
Then added daily according to promoter concentration 0.01-1mg/L in sewage.
7. the application of accelerating agent according to claim 5, it is characterised in that：In use, 20-40 DEG C of temperature is controlled,
PH is 6.0-9.0, dissolved oxygen 1.0-5.0mg/L.
8. according to the application of the accelerating agent of claim 5 or 6, it is characterised in that：It adds simultaneously in use a certain amount of
Auxiliary agent, auxiliary agent is one or both of polyamines, inorganic acid azanol.
9. application according to claim 8, it is characterised in that：Polyamines are the mixed of spermine, spermidine or both
Close object；Inorganic acid azanol is one or more of hydroxylamine hydrochloride, hydroxyl sulfate or phosphatic hydroxylamine etc..
10. application according to claim 8, it is characterised in that：The dosage of the auxiliary agent is the 0.1- of promoter concentration
0.5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611072922.9A CN108118021B (en) | 2016-11-29 | 2016-11-29 | Microbial growth promoter for completing nitration process and application thereof |
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|---|---|---|---|
| CN201611072922.9A CN108118021B (en) | 2016-11-29 | 2016-11-29 | Microbial growth promoter for completing nitration process and application thereof |
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| CN108118021A true CN108118021A (en) | 2018-06-05 |
| CN108118021B CN108118021B (en) | 2020-11-10 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111018104A (en) * | 2019-12-17 | 2020-04-17 | 南京大学 | Application of mannitol as additive in relieving salinity impact of anaerobic ammonia oxidation reactor |
| CN111672471A (en) * | 2020-06-22 | 2020-09-18 | 陈苗根 | Biological activated carbon capable of improving COD removal efficiency and preparation method and application thereof |
| CN114807001A (en) * | 2021-06-08 | 2022-07-29 | 上海未来企业股份有限公司 | Promoter beneficial to rapid propagation of nitrobacteria and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN111672471A (en) * | 2020-06-22 | 2020-09-18 | 陈苗根 | Biological activated carbon capable of improving COD removal efficiency and preparation method and application thereof |
| CN114807001A (en) * | 2021-06-08 | 2022-07-29 | 上海未来企业股份有限公司 | Promoter beneficial to rapid propagation of nitrobacteria and preparation method thereof |
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| CN108118021B (en) | 2020-11-10 |
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