KR100237972B1 - Liquid microbe disposal agent for disposing of waste water - Google Patents

Abstract

The present invention relates to a microbial culture material for wastewater treatment and a method of using the same, and more particularly, to a microbial culture material containing an active microorganism group useful for industrial wastewater, closed sewage purification tank, anaerobic digestion tank purification and livestock wastewater treatment. .

SUMMARY OF THE INVENTION An object of the present invention is to provide an active microbial culture material in which organic materials in wastewater and manure are decomposed, and cultured a microorganism group having various poorly soluble substances and odor removal ability. It is another object of the present invention to provide a method for efficiently and economically removing organic wastewater and livestock manure wastewater using such active microbial culture.

The microorganism culture material according to the present invention is a group of microorganisms having the ability to decompose organic substances and various hardly decomposable substances in the wastewater, and to remove sulfides such as hydrogen sulfide, methyl mercaptan, and causative agents of eutrophication such as organic phosphorus and ammonia nitrogen. By culturing the active microorganism culture material to improve the activity while maintaining the preservation and stability of the microorganism, it can be used for effective wastewater treatment.

Description

Liquid microbial treatment agent for wastewater treatment

The present invention relates to a liquid microbial treatment agent for wastewater treatment, and more particularly to a microbial treatment agent containing an active microbial group useful for industrial wastewater, closed sewage treatment tank, anaerobic digestion tank and livestock wastewater treatment.

Representative methods for treating organic matter in wastewater and manure are biological treatment methods using a reaction tank (hereinafter referred to as aeration tank), and biologically active sludge that decomposes organic substances by acting on various kinds of microorganisms, ie, activated sludge, in the aeration tank. Methods and the like are used. The biological treatment method using the aeration tank can decompose various organic materials and has good treatment efficiency, but it requires skilled management skills and the treatment efficiency easily decreases according to load fluctuations. There is a problem that not only the risk of stopping the microorganisms and stopping the wastewater treatment but also the bulking phenomenon occurs even if the temperature and organic concentration are maintained in a stable range and aeration. In addition, this activated sludge method acts to reduce biological oxygen demand (BOD) and chemical oxygen demand (COD) by cultivating dozens of sludges in wastewater and manure to magnetize organic carbon and organic nitrogen sources. The law has several problems in wastewater treatment. The biggest problem among them is that it does not remove hardly decomposable substances, odorous substances (H ₂ S, CH ₃ CH, etc.), organophosphorus and ammonia nitrogen. That is, when the above substances are discharged into rivers and seas, they become main causes of odor and eutrophication.

At present, many kinds of microbial agents are used in Korea for the purpose of solving the problems of the above-mentioned biological treatment method using aeration tank, and the manufacturing method thereof is described in Korean Patent Publication No. 92-18406 (Invention) : Polypeptides), 90-14258 (name of the invention: a method for producing a waste water purification agent using a microorganism) and Patent Publication No. 91-4083 (name of the invention: a method for producing a microbial nutritional composition for waste water treatment) It is described.

The effluent purification microbial agent is mainly used in a solid form, the solid microbial agent is superior in the preservation and stability of the microorganisms compared to the liquid microbial agent, there is a problem that the activity is inferior.

In order to solve the above problems of activated sludge treatment, the present inventors investigated various organic substances, poorly soluble substances, and odor removing ability of known strains, and developed a complex fermentation technology capable of simultaneously cultivating several strains by pure separation. The present invention has been completed.

In addition, the inventors of the present invention in consideration of the problem that the wastewater treatment depends on the activity of the living microorganisms, while conducting research to solve the problem of the removal of solid microorganisms, by cultivating a specific group of microorganisms in a fermentation medium to maintain the preservation and stability of the microorganisms While using the microbial culture material to improve the activity while finding out that the wastewater treatment can be effectively completed the present invention. Accordingly, an object of the present invention is to cultivate a group of microorganisms having the ability to remove organic substances and various hardly decomposable substances in wastewater and manure, sulfides such as hydrogen sulfide, methyl mercaptan, and agents causing eutrophication such as organic phosphorus and ammonia nitrogen. It is to provide an active microorganism culture material.

Still another object of the present invention is to provide a method for efficiently and economically removing organic wastewater and livestock manure wastewater using the active microorganism culture material.

1 shows the growth and oxygen demand of microorganisms.

The microorganisms contained in the treatment agent according to the present invention include (1) undigested protein in wastewater, microorganisms that break down lipids, (2) microorganisms that break down fibers, (3) microorganisms that break down carbohydrates and organic compounds, ( 4) Nitride microorganisms that fix nitrogen in air and water, and (5) Microorganisms that degrade hardly decomposable substances in wastewater. These microorganisms may be used as a single species depending on their application, but microorganism culture materials in which several kinds of microorganisms are adsorbed and cultured, and preferably all of them are mixed and cultured simultaneously can be used as a treatment agent.

The microorganisms of the above (1) to (5) are all known strains, and each microorganism obtained by purely separating and culturing aerobic and random bacteria having excellent degradability in wastewater treatment among bacteria existing in soil, river, and river goes through a growth stage. It is a strain whose growth is at rest. Proteins and lipolytic microorganisms are of the genus Bacillus sp. And microorganisms containing cellulase enzymes that break down fiber are Cellulomonas sp., Carbohydrates and ABS, hexane, phenol , Pseudomonas sp., Rhodopseudomonas sp., Microorganisms that decompose organic compounds, such as hydrocarbons, and Nitrosomonas sp. (Nitrobacter sp) and Enterobacter sp., Corynebacterium sp. (Corynebacterium sp.), Micrococcus (to promote the decomposition of hardly degradable substances (leather, resin, dyeing wastewater) contained in wastewater). It is composed of 8 microbial groups including Micrococcus sp.

Microbial cultures are nutrients supplied to prevent microorganisms from deactivating when they are required for long-term preservation.They are low carbohydrates such as glucose, sugar, sucrose, or lactose, and low fatty acids such as acetate, propionate, malate, and butyrate. The contained material is used.

The viable cell count of the microbial culture material according to the present invention is as follows.

Accurately weigh 1 g of sample, add 9 ml of 0.7% saline, and mix well. Take 1 ml of the mixed solution and dilute continuously with 9 ml saline.

Each serial dilution mixture is aseptically taken in 0.1 ml of each dilution factor and plated on a nutritious agar plate (Nutrient agar plate; beef extract 3g, peptone 5g, agar 15g distilled water 1L, pH 6.8).

The plate is incubated for 3 days in a thermostat.

The colonies (colony) formed on the plate are measured.

If 100 colonies were generated at the dilution of 1: 1 × 10 ⁸ times, the number of viable cells is 100 × 10 ⁸ / g using the following equation.

Viable cell count = microbial colony × dilution factor × 10 (dog / g)

The microbial agent according to the present invention is a liquid microbial treatment agent which is selected and mixed with a good strain, and maintains high activity and viable cell number, thereby preventing the killing of microorganisms due to circulation, and immediately administering the microbial agent to the wastewater without an activation step. Therefore, it is possible to exert an excellent effect in the wastewater treatment step in removing the eutrophication substances that cause the river pollution or the coastal pollution by rapidly decomposing organic substances.

Another feature of the present invention is to minimize the microbial lag phase (15 seconds) to immediately develop the activity in the wastewater to maximize the effect of the wastewater treatment in the shortest time.

Hereinafter, the present invention will be described in detail by way of examples.

Example 1

Known strains Bacillus subtilus, Cellulomonas cellulans, Pseudomonas aeruginosa, Rhodopseudomonas gelatinosa, Nitrosomana eurospapa europa ), Nitrobacter winogradski, Enterobacter aerogenes, Corynebacterium acetoglutamicum, and Micrococcus glutamicus agar on the agar plate Pure isolates and passages were selected to select strains with excellent material resolution. The results of measuring the material degradation ability of the selected microbial group are shown in Table 1 below.

Example 2

Each microorganism liquid isolated and cultured in Example 1 and the obtained microbial group were placed in a nutrient broth at pH 6.8 for 12 hours with known microorganisms (8 species), 3 g of beef extract, 5 g of peptone, and 15 g of agar. 1 l of distilled water was mixed and cultured at 30-35 ° C., and 1 g of the powdered microbial agent obtained by hot-air drying the culture solution was inoculated into waste water having a BOD of 1,000 ppm to measure growth activity. The results are shown in Table 2 below.

Example 3

1 ml of the liquid microbial agent prepared in Example 2 and 1 g of the powdered microorganism obtained by hot-air drying the culture medium were inoculated into a 100 ml flask of Yeast Malt Brpth medium, and incubated at 30 ° C. for 1 day, and the number of viable cells of the culture medium was changed for each time period. Measured. The results are shown in Table 3 below.

The composition of the yeast malt medium is as follows.

Yeast Extract 3g, Malt Extract 3g, Peptone 5g, Dextrose 10g, Distilled Water 1L, pH 6.2

Example 4

100 ml of the microbial culture solution prepared in Example 1 was administered to the wastewater treatment aeration tank to adjust the pH in the aeration tank to 6.5-7.0, and then the sludge amount, BOD and COD were measured for 7 days at a daily interval. It is shown in Table 4 below in comparison with the activated sludge method.

Example 5

100 ml of powder was collected from manure wastewater, mixed with artificial wastewater, and then 400 ml of artificial serving mixture was prepared, followed by aerobic treatment for 2 days. 50 g of this was inoculated with 10 ml (5 × 10 ⁸ cells / ml) of the microbial culture prepared in Example 1, and then placed in a 100 ml flask and cultured at 35 ° C. under anaerobic conditions.

The results of daily measurement of malodorous hydrogen (H ₂ S) and methyl mercaptan (CH ₃ SH) as odor components in the flask inoculated with the microorganism culture medium and the flask not inoculated are shown in Table 5 below.

Example 6

As in Example 5, the results of analysis of organophosphorus and ammonia nitrogen, which are the causes of eutrophication, are shown in Table 6 below.

The treatment agent of the present invention is not only easily and cheaply manufactured, but also acts as a stronger water purifier than the conventional activated sludge method, so that the organic substances contaminated by sewage, industrial wastewater, domestic wastewater, etc. make eutrophication materials, and thus the ecosystem of malt and plankton. It can easily solve the pollution caused by the destruction of the water, or the pollution caused by red tide or heavy metals, effectively purifying water quality below the legal standard. In addition, the treatment agent of the present invention can minimize the induction phase (lag phase) of microbial growth as shown in Table 2 and Table 3 to immediately treat the liquid microbial culture in the wastewater can obtain the wastewater treatment effect in the shortest time.

On the other hand, the microorganisms in the culture material according to the present invention can be grown even in aerobic state to continue to decompose harmful odorous substances of the manure to remove odors. In particular, odorous substances from livestock excretion are metabolites that are decomposed and degraded by anaerobic bacteria under anaerobic conditions, and these metabolites are grown while photosynthetic bacteria are ingested. In particular, lower fatty acids, which are the main causes of odor, are substances that are used as carbon sources for microorganisms and are completely removed after 2 to 3 days in aerobic state and 5 days in anaerobic state to completely inhibit the growth of anaerobic bacteria using these substances as nutrients. As a result, as shown in Table 5 and Table 6, sulfides such as hydrogen sulfide and methyl mercaptan, and generation of eutrophic agents such as organophosphorus and ammonia nitrogen are suppressed.

In addition, in order to purify industrial wastewater, livestock wastewater, etc., there is a demand for reducing power consumption by reducing the processing time due to the large amount of power used in wastewater treatment plants. Decomposition of the decomposable substances greatly reduces the treatment time.

Claims (1)

Wastewater treatment agent using microorganisms, Bacillus subtilis as protein and lipolytic microorganisms, Cellulomonas cellulose as fibrolytic microorganisms, Pseudomonas aeruginosa or Rhodoshudomonas gelatinosa as carbohydrate-decomposing microorganisms, Is a mixed culture of microorganisms consisting of Enterobacter aerogenes, Corinobacterium acetoglutamcum, or Micrococcus glutamicus, as the microorganisms of nitrosomonas europaea or nitrobacter winogradsky, and the biodegradable substances in wastewater. Liquid microbial treatment agent for wastewater treatment, characterized in that.