KR0165526B1 - Preparation process of waste water treating agent - Google Patents

Abstract

1. TECHNICAL FIELD OF THE INVENTION

The present invention relates to a method for producing a wastewater treatment agent containing microorganisms.

2. The technical problem to be solved by the invention

Conventional wastewater treatment agents containing microorganisms have a long treatment time of the wastewater, and due to the poor settling of the resulting flocks, there is a problem that the flocks containing the microorganisms and the suspended solids are discharged together.

3. Summary of Solution to Invention

The present invention is to solve the problems as described above, suspended by shaking the soil collected in the starch factory, pig farm, slaughterhouse surroundings in sterile water, and then plated in sterile plate medium and incubated at 30 to 37 ℃ for 4 to 5 days 1st process to do,

A second step of culturing the liquid 0.1 to 0.3 parts by weight of the cultured in the first step in a culture medium and culturing the liquid for 18 to 24 hours at 30 to 37 ° C,

Sterilized solid medium consisting of bran, rice bran, sawdust, elvan powder, ground flour, glucose and corn powder, the culture medium of the second step was mixed at 40% (w / w) with respect to the solid medium, The third step of culturing for 3 to 4 days,

After collecting the soil around the old tree and shaking it with sterile water and suspended it, spread it on sterilized plate medium and incubate for 5 to 7 days at 30 to 35 ° C. A fifth step of incubating 0.3 parts by weight in a culture solution and incubating the liquid at 30 ° C. for 3 days,

The culture medium prepared in step 5 was mixed with solid medium sterilized by mixing starch, bran, sawdust, perilla jelly and ganban stone powder at 50 to 60% (w / w) with respect to the solid medium. A sixth process of incubating the solid for 5 days;

30 parts by weight of the culture medium prepared in the third step and 20 parts by weight of the culture medium prepared in the sixth step is mixed with 50 parts by weight of the mixed culture pulverized sufficiently dry, 30 parts by weight of ganban stone and 5 parts by weight of lactose The above problem can be solved by using the wastewater treatment agent prepared by the process to the seventh process.

4. Important uses of the invention

The wastewater treatment agent prepared according to the present invention is used as a wastewater treatment agent containing a large amount of organic material.

Description

Method for manufacturing wastewater treatment agent

The present invention relates to a method for manufacturing a wastewater treatment agent using microorganisms, and more specifically, to the activity of microorganisms obtained by culturing microorganisms collected from soils of slaughterhouses, starch plants, pig farms, old trees, etc. using enzymes and porous materials. The present invention relates to a method for preparing a wastewater treatment agent which increases treatment efficiency by increasing floc and flocculation stability of flock formed by microorganisms in an aeration tank during wastewater purification.

Wastewater treatment can be divided into chemical treatment, physical treatment, and biological treatment according to the type of pollutants contained in the wastewater. However, the main treatment process of organic pollutant removal in most wastewater treatment plants uses microorganisms. Since biological treatment methods are used, microorganisms in wastewater treatment occupy a very important position.

In general, the process of biological wastewater treatment by microorganisms, the organic contaminants in the wastewater is decomposed and absorbed by bacteria microorganisms, such as bacillus (nicillus), nitrosomonas (pseudomonas), aerobacactor (aerobactor) The microorganisms propagated by protozoa such as vorticella, opermularia, aspedesca, rotaria, carchesium, episthylis, etc. The food chain is ingested and processed by a series of processes in which flocks are formed and precipitated and separated together with undecomposed contaminants, suspended matter, viscous substances (Zooglea tamigera) and microorganisms.

In the wastewater treatment process by these microorganisms, the selection of the microorganisms according to the characteristics of the wastewater and the aggregation of flock formed in the wastewater treatment process by the selected microorganisms have a great influence on the contaminant decomposition ability of the microorganisms. If the flock is tightly packed with cancer, the organic food chains of bacteria and protozoa are maintained smoothly, increasing their levels to improve the ability to remove contaminants, but the growth of microorganisms in the wastewater treatment plant. When the environment is changed and the flock is dismantled, bacteria and protozoa are killed, which reduces the ability to decompose pollutants and lowers the efficiency of wastewater treatment.

In addition, when the flock is dismantled, contaminants do not settle and become suspended and are discharged together with the treated water, which may cause secondary water pollution.

Most producers equipped with wastewater treatment facilities construct wastewater treatment plants based on the amount of wastewater calculated based on product types and production volume at the beginning of production, so that microorganisms in the wastewater treatment plant are at the time of construction. Although it is possible to maintain a complete ecosystem of food and food chains, microbial microecosystems in the wastewater treatment plant are easily destroyed when changes in the production process of the product or the quantity of the wastewater flowing into the wastewater treatment plant change as the production volume increases. As the flock is dismantled, the levels of microorganisms and protozoa are relatively reduced, and the wastewater treatment efficiency is drastically reduced.

In such a case, it is desirable to keep the influent wastewater in the wastewater treatment plant for a long time to multiply the microorganisms to the absolute value required to treat the influent wastewater. However, there is a problem in that the volume of the wastewater treatment plant is limited, and therefore, it is very difficult to accept the increased amount of wastewater for a sufficient time in the treatment plant itself.

The above problems can be solved by shortening the recovery time of microbial levels by directly injecting the microorganisms and trace elements into the wastewater treatment plant. (Flock) and suspended solids were discharged together.

Even in this case, the wastewater treatment plant must be kept for a long time before the discharged water is discharged to settle the suspended solids sufficiently with microbial flock.Also, due to the volumetric limitation of the wastewater treatment plant, it is practically difficult to receive the treated water for a sufficient time. There was a problem.

The present invention can accelerate the decomposition of organic contaminants of various properties newly introduced into the wastewater treatment plant using various kinds of organic matter degrading enzymes, and by culturing microorganisms useful for wastewater treatment using porous materials, By strengthening the cohesive force of the flock formed when the decomposed pollutants are processed by the microorganism, it is possible to stably maintain the organic food chain relationship between the microorganism and the protozoa even if the growth environment of the microorganism changes frequently. The purpose of the present invention is to provide a wastewater treatment agent that can prevent secondary contamination of treated water by suspended solids when the microorganisms are used directly in the wastewater by improving the sedimentation stability of the flock.

The present invention consists of the following first to seventh step, by using the ganban stone as a porous material to cultivate the microorganisms useful for wastewater treatment to supply microorganisms with inorganic nutrients and to accelerate the sedimentation rate during wastewater treatment It is characterized by the preparation of wastewater treatment agent.

In the first process, the soil collected from the starch plant, pig farm, slaughterhouse is shaken by suspending in sterile water and then suspended in sterilized plate medium and incubated at 30 to 37 ° C. for 4 to 5 days to produce Pseudomonas, micro Selective cultivation of microorganisms useful for wastewater treatment, such as Micrococcus, Flabobacterium, Bacillus, and the like. Nutrient agar having a pH of 7.2, containing 0.2 to 0.5 parts by weight of K ₂ HPO _4, 0.1 to 0.5 parts by weight of MgSO _4, 0.1 to 0.5 parts of Egg-Albumin, and 15 parts by weight of Agar. (Nutrient agar) Reputation badge.

In the second step, 0.1 to 0.3 parts by weight of the bacterium cultured in the first step is seeded in the culture medium, followed by enrichment for 18 to 24 hours at 30 to 37 ° C. The culture medium in the second step is also a general liquid culture solution. 1000 parts by weight of K ₂ HPO ₄ 1 to 2 parts by weight, MnSO ₄ 0.5 to 1 part by weight, MgSO ₄ 0.5 to 1 part by weight of glucose 3 to 10 parts by weight and soybean meal 5 to 20 parts by weight of the sterilized solution do.

The third process consists of 5 to 10 parts by weight of bran, 5 to 10 parts by weight of rice bran, 2 to 5 parts by weight of sawdust, and 1 to 3 parts by weight of crushed ganbanite powder and granules of 0.3 to 1 part by weight and 0.5 of glucose. To 1 part by weight and 1 to 2 parts by weight of jade powder (corn powder), the culture medium prepared in the second step is mixed with 40% (w / w) to a solid mixture such as bran and 30 to 30 It is a process for producing protease, carbohydrate amylase, and lipolytic enzyme, proteolytic microorganism, carbohydrate microorganism, and lipolytic microorganism by culturing for 3-4 days at 35 ℃. The prepared microbial aggregate is used in the seventh step.

The fourth step is a separate process from the first to the third step, take the soil around the old tree and shake it with sterile water and suspend it, smear it on a sterilized plate medium and apply for 5 to 7 days at 30 to 35 ℃. Cultivation of microorganisms useful for wastewater treatment such as Bacillus, Nitrosomonas, Pseudomonas, etc., by culturing the plate medium used in the fourth step, the plate medium used in the first step Is the same commonly known medium as

The fifth step is a step of spawning 0.1 to 0.3 parts by weight of the fungi prepared in the fourth step in a culture medium and enrichment culture at 30 ° C. for 3 days. The culture medium used in this process is 1000 parts by weight of distilled water and 5 to 20 parts by weight of glucose. with peptone (peptone) 1 to 5 parts by weight of K ₂ HPO ₄ 0.5 to 1.5 parts by weight MnSO ₄ 0.5 to 1 parts by weight of MgSO ₄ 0.1 to 0.5 by mixing parts by weight of rice bran, 100 and parts of 300 parts by weight the solution was sterilized .

In the sixth process, 2 to 5 parts by weight of starch, 10 to 15 parts by weight of bran, 2 to 5 parts by weight of sawdust, 5 to 10 parts by weight of perilla cake and 28 to 32 parts by weight of pulverized gantherite powder After sterilization, the culture solution prepared in the fifth step is mixed at 50 to 60% (w / w) of the weight of the solid mixture such as starch, and cultured at 30 to 40 ° C. for 4 to 5 days to obtain a fibrinolytic enzyme (Cellulase) and Fibrous decomposing microorganisms and nitrogen compound decomposing microorganisms are prepared and transferred to the seventh step.

The seventh step is the final step of the present invention, 50 parts by weight of the mixed culture cultured in a particle size of 80 to 100 mesh mixed with 30 parts by weight of the culture medium prepared in the third step and 20 parts by weight of the culture medium prepared in the sixth step and then sufficiently dried To prepare a waste water purification agent in accordance with the above purpose by mixing 30 parts by weight of ganban stone and 5 parts by weight of lactose, which are ground to a particle size of 100 to 350 mesh.

As can be seen from the first to seventh step, in the present invention, after incubating with a liquid medium unlike the conventional process, it can be seen that the enrichment is again performed using a solid medium. That is, conventionally, liquid medium was mainly used, and some solid medium was used, but when only liquid medium was used, there was a problem of evaporating water after enrichment. There was a hassle to do.

In the present invention, it is another feature to eliminate the above inconvenience by mixing the culture medium of the liquid culture as it is mixed with the solid medium and enriched.

In addition, elvan stones used in the third, sixth, and seventh processes are generally used in the field of water treatment, and have been mainly used to elute inorganic substances by ion exchange. 60% SiO ₂ , Al ₂ O ₃ 16 It is a porous mineral composed of inorganic materials necessary for the growth of microorganisms such as%, FeO 2%, Fe ₂ O ₃ 2%, CaO 1.5%, MgO ₂ 4.5%, NaO ₂ 3%, K ₂ O 3%.

In the present invention, when a variety of organic matter degrading enzymes and microorganisms prepared by the steps 1 to 4 are filled in the cavity of the elvan, the microorganisms can be sufficiently supplied with the inorganic trace elements necessary for growth from the elvan, and stably multiply. Flock is made to have a dark density because it can further enhance the cohesion of the flock formed by maintaining a stable food chain relationship with protozoa.

In addition, the flock formed as a trace element acts as a trace element and the remaining minerals act as a weight, which has a settling stability, thereby accelerating the sedimentation rate, and also reduces the volume of the precipitate due to an increase in the specific gravity of the flock itself. Will be done.

In case of using the elvan, which has such a function as a large particle size of 100 mesh or less, the surface area of the cavity is reduced, the amount of inorganic trace elements eluted from the elvan is reduced, and the growth space of the microorganism is reduced, thereby reducing the growth rate of the microorganism. Wastewater purification efficiency is lowered, and when used with a small particle size of 350 mesh or more, rather than delaying the flocculation time formed during decomposition, the sedimentability is lowered. It is suitable.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples.

EXAMPLE

[Step 1]

Take 1g of soil from starch plant, pig farm, slaughterhouse, mix, suspend and mix with 100ml of sterile water, and then heat at 125 ℃ for about 15 minutes to sterilize Nutrient Agar plate. The cells were plated and cultured for 5 days while maintaining a temperature of 35 ± 2 ° C. to culture cells containing large amounts of Pseudomonas, Micrococcus, Flavobacterium, Bacillus, and the like.

[Step 2]

The gyunrak 0.3g containing Pseudomonas (Pseudomonas), micro Rhodococcus (Micrococcus), Flavobacterium (Flavobacterium), Bacillus (Bacillus), such as from the first process, distilled water to 1000ml, and K ₂ HPO ₄ 2g 1g and ₄ and MnSO 1 g of MgSO _4, 10 g of glucose, and 15 g of soybean meal were mixed, sterilized at 100 ° C. for 30 minutes, and seeded in a solution cooled to 40 ° C., and incubated for 20 hours while maintaining a temperature of 35 ± 2 ° C.

[Step 3]

10 g of bran, 10 g of rice bran, 5 g of sawdust, and 300 mesh of granulated ganbanite, 1 g of ground flour, 1 g of glucose, 1 g of glucose, and 2 g of oxygen were cooled and sterilized at 40 ° C., followed by 21 ml of the culture solution prepared in the second step. The mixture was incubated for 4 days while maintaining a temperature of 32 ± 2 ℃.

[Step 4]

1 g of soil is collected from the dead tree, mixed with 100 ml of sterile water and suspended in suspension, and then plated on the same sterilized Nutrient Agar plate medium as the medium used in the first step. By culturing daily, cells containing Bacillus, Nitrosomonas, Pseudomonas, and the like were cultured.

[Step 5]

Bacillus from the fourth step (Bacillus), nitro consumption eggplant (Nitrosomonas), Pseudomonas (Pseudomonas) 1000ml distilled water and K ₂ HPO gyunryak a 0.3g containing such ₄ and MnSO _4, 1.5g MgSO ₄ 1g and 0.5g and 20g glucose After mixing 200g of rice bran and sterilization for 30 minutes at 100 ℃ sterilized in a solution cooled to 40 ℃, and incubated for 20 hours while maintaining a temperature of 35 ± 2 ℃.

[Step 6]

15 g of bran, 5 g of starch, and 30 g of crushed ganbanite, and 10 g of perilla jelly and 5 g of sawdust were steam sterilized and cooled to 40 ° C., followed by mixing 79 ml of the culture solution prepared in step 5 above. Incubated for 5 days while maintaining the temperature of ± 2 ℃.

[Step 7]

After drying the cultures prepared in the third step and the sixth step for 30 hours, the mixture was pulverized into 30 mesh and 30 g respectively, and then pulverized to 90 mesh, and then mixed with 30 g of ganbanite and 5 g of lactose with 85 g of wastewater. Treating agent was prepared.

Experimental Example

From a food processing plant near Seoul, the active wastewater was collected by the wastewater and microorganisms, which are being purified.

The wastewater treatment agent prepared in the above example was mixed with the raw wastewater and the active wastewater at a volume ratio of 3: 7, and then 1 liter each was added to two culture vessels made of transparent acrylic plates and two 1 liter volumetric cylinders. BOD (Biochemical Oxygen Demand), COD (Chemical Oxygen Demand) and SV ₃₀ (1 liter mass cylinder in culture vessel) after addition at 50 ppm and purged for 8 hours, 16 hours and 24 hours respectively according to the Pollution Process Test Method The volume (ml) precipitated for 30 minutes was measured, and the type and number of protozoa that appeared were observed under a microscope and the results are shown in Table 1 below.

Initial BOD (biological oxygen demand), COD (chemical oxygen demand), SV ₃₀ (sustained matter in the culture tank precipitated for 30 minutes in a 1 liter measuring cylinder) of the mixed waste water mixed with the original wastewater and the active wastewater used in the experimental example of the present invention. Volume (ml), pH, MLSS (suspension in culture vessel), DO (dissolved oxygen content) values and temperatures were as follows.

BOD: 720 ppm MLSS: 3000 ppm

COD: 500 ppm pH: 7.5

SV ₃₀ : 80 ml temperature: 25 ℃,

[Comparative Example]

After mixing the raw wastewater and the active wastewater in the same manner as in the above experimental example, after purifying for 8 hours, 16 hours, 24 hours without adding the wastewater treatment agent of the present invention prepared in the above embodiment, pollution process test The BOD, COD, and SV ₃₀ were measured according to the method, and the types and values of protozoa that appeared were shown in Table 1 below.

(The numbers indicated after the protozoan names in Table 1 above represent the comparative values based on 4 of the protozoan species found during microscopic observation.)

As shown in Table 1, the BOD and COD values after 24 hours after the addition of the wastewater correction agent according to the present invention are about 30 ppm smaller than those without the addition of the wastewater correction agent according to the present invention. The wastewater purification effect was found to be remarkably good.

In addition, it can be seen that the SV value in the experimental example is about 23% smaller than the SV value in the comparative example.

When the wastewater treatment agent of the present invention is used, the dark density and sedimentation of the flock formed during the decomposition process are better than those without the wastewater treatment agent of the present invention, so that the treatment is caused by the flotation of the floc. This proves that the problem of water secondary pollution can be greatly improved.

The above results are also evidenced by the type and number of protozoa present during the purification process.

That is, in the case of the experimental example, as well as Vorticella, as the purification time passes, various kinds of protozoa such as Aspidisca, Epistylis, Carchesium, Rotaria, etc. Not only did the animals appear but also the population gradually increased, but in the comparative example, there was no significant change in the number and number of protozoa.

The present invention can effectively cope with newly introduced organic contaminants of various properties into the wastewater treatment plant, and enhance the cohesiveness and sedimentation stability of the flock formed during the wastewater treatment by the microorganisms, thereby frequently growing microorganisms. It is a useful invention that can stably maintain wastewater treatment efficiency even with environmental changes.

Claims (5)

A method for producing a wastewater treatment agent comprising the following first to seventh steps. The soil collected from the starch plant, pig farms, slaughterhouses around the suspension by shaking with sterile water, the first step of spreading the sterilized plate medium and incubated for 4 to 5 days at 30 to 37 ℃, the culture in the first step 0.1 to 0.3 parts by weight of the resulting bacterium was added to the culture medium, followed by a second step of liquid culturing at 30 to 37 ° C. for 18 to 24 hours, and sterile treatment consisting of wheat bran, rice bran, sawdust, ganban stone powder, flour, glucose and corn powder. In the solid medium, the third step of mixing the culture medium of the second step at 40% (w / w) with respect to the solid medium and incubated for 3 to 4 days at 30 to 35 ℃, and the soil around the old tree is sterile water After the suspension was shaken in a suspension, plated on sterilized plate medium and incubated for 5 to 7 days at 30 to 35 ° C., and 0.1 to 0.3 parts by weight of the fungi cultured from the fourth step were seeded in a culture medium, followed by 3 at 30 ° C. Fifth cultured for days The culture medium prepared in the fifth step is mixed with a solid medium sterilized by mixing the process, starch, bran, sawdust, perilla jelly and ganban stone powder at 50 to 60% (w / w) with respect to the solid medium and 30 to 40 50 parts by weight of the mixed culture, which is sufficiently dried and ground by mixing the sixth step of solid culture at 4 ° C. for 5 days with 30 parts by weight of the culture medium prepared in the third step and 20 parts by weight of the culture medium prepared in the sixth step, 30 parts by weight of elvan rock and 5 parts by weight of lactose. According to claim 1, wherein the solid medium of the third step is 5 to 10 parts by weight of wheat bran, 5 to 10 parts by weight of rice bran, 2 to 5 parts by weight of sawdust, 1 to 3 parts by weight of ganbanite powder and 0.3 to 1 part by weight of glucose and glucose A method for producing a wastewater treatment agent, characterized in that it consists of 0.5 to 1 part by weight and 1 to 2 parts by weight of corn flour (corn powder). The solid medium of the sixth step is 2 to 5 parts by weight of starch, 10 to 15 parts by weight of wheat bran, 2 to 5 parts by weight of sawdust, 5 to 10 parts by weight of perilla jelly and 28 to 11 parts of granule powder. Method for producing a wastewater treatment agent, characterized in that consisting of 32 parts by weight. The method of claim 3, wherein the particle size of the culture mixture is 80 to 100 mesh. The method for producing a wastewater treatment agent according to claim 4, wherein the particle size of the elvan powder is 100 to 350 mesh.