KR880000583B1 - Preparation for flock accelator of waste water - Google Patents

Preparation for flock accelator of waste water Download PDF

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KR880000583B1
KR880000583B1 KR1019850003029A KR850003029A KR880000583B1 KR 880000583 B1 KR880000583 B1 KR 880000583B1 KR 1019850003029 A KR1019850003029 A KR 1019850003029A KR 850003029 A KR850003029 A KR 850003029A KR 880000583 B1 KR880000583 B1 KR 880000583B1
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wastewater
waste water
bacillus
rice bran
flock
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KR860008945A (en
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도만영
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도만영
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
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  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
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  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cereal-Derived Products (AREA)
  • Activated Sludge Processes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The nutrient composition of activated sludge for the treatment of waste water is prepd. by mixing fermentive materials, inoculated rice bran with bacillus substillus and bacillus natto, with the cultured material of yeast, and then adding some quantity of MgSo4, FeSo4 and CaCl2 to their mixture. In the process, COD, BOD and a pigment contained in waste water are decomposed within about 12-24hr, and an alkaline waste water of PH 7.0-10.5 can be effectively treated with the process.

Description

폐수처리용 활성오니 영양제 조성물의 제조방법Method for preparing activated sludge nutrient composition for wastewater treatment

산업의 급속적인 발달과 함께 심각성을 띄고 있는 악성 폐수의 유출로 인한 환경의 오염문제가 제기된지도 오랜 세월이 지나왔다.It has been a long time since the rapid development of the industry caused the pollution of the environment caused by the outflow of malignant wastewater, which is serious.

이러한 환경오염의 주된 원인중의 하나인 공업폐수 및 도시하수등은 우리 인간들의 중요한 식수원인 하천과 강을 극심하게 오염시키므로서, 우리국민의 보건을 위협하고 있음은 주지된 사실이다.It is well known that industrial wastewater and urban sewage, which are one of the main causes of environmental pollution, seriously pollute rivers and rivers, which are important sources of drinking water for humans, and thus threaten the health of our people.

이러한 폐기물질을 처리하기 위한 벙법으로는 화학적인 방법, 물질적인 방법, 또는 미생물학적인 방법등으로 크게 구분하여 볼수가 있는 것이다.Methods for treating such waste materials can be classified into chemical, material, or microbiological methods.

본 발명은 이상의 방법중에 미생물학적인 방법에 의하여 폐수를 처리할 수 있도록한 것으로, 3종류의 미생물을 특정 조건에서 배양하여 이들을 혼합한 폐수 처리용 활성오니 영양조성물의 제조방법에 관한 것이다.The present invention enables treatment of wastewater by a microbiological method among the above methods, and relates to a method for producing activated sludge nutrition composition for wastewater treatment by culturing three kinds of microorganisms under specific conditions and mixing them.

오염된 폐수가 계속 하수로 등을 통하여 흘러가는 동안에 수중에 함침되어 있는 미생물이 외부의 공기로부터 산소를 공급받아 활동하므로써 오염된 물 속에 포함된 유기물을 분해 변화시켜 주므로서 물이 정화케되는 것이다.As contaminated wastewater continues to flow through sewers, the microorganisms impregnated in the water are purged by decomposing and changing organic matter contained in the contaminated water by receiving oxygen from the outside air.

이와같은 오염수의 정화원리를 인위적으로 재현시켜 이용하는 것이 활성오니법(미생물학적 처리)이라 한다.Artificially reproducing the principle of purification of contaminated water is called an active sludge method (microbial treatment).

활성오니법에서 관찰할 수 있는 미생물의 종류를 분류하여 보면, (1)세균류 (2)원생동물류 (3)후생동물류 등과 같은 3종류의 형태로 크게 분류할 수 있는 것인데, 상기한 활성오니 생물 중 특히 세균류가 오염수중의 유기물을 무기물로 분해하여 안정화 시키고, 침강성이 양호한 덩어리(FLOCK)를 형성 시키는데 가장 중요한 역활을 하고 있음은 이미 문헌에 기술된 주지의 사실이다.The types of microorganisms that can be observed in the activated sludge method can be classified into three types, such as (1) bacteria, (2) protozoa, and (3) welfare animals. In particular, it is a well-known fact already described in the literature that bacteria play the most important role in decomposing and stabilizing organic matter in contaminated water to inorganic matter and forming a good settling floc.

폐수중에서 미생물의 활동 상태를 단계적으로 살펴보면 다음과 같다.Step-by-step look at the state of activity of microorganisms in wastewater.

제1단계 : 폐수중의 미세한 입자가 많기 때문에 이에 적응하여 활동하는 박테리아 세균류가 발생하게 된다.First step: Since there are a lot of fine particles in the wastewater, bacteria and bacteria that adapt and act on them are generated.

제2단계 : 이들 박테리아류를 섭취하는데 적당한 모양의 편모충류나 유영형의 섬모충류등이 상당하게 자연 증식된다.The second stage: In order to consume these bacteria, flagella worms and swimming-type ciliated insects, which are appropriately shaped, grow considerably.

제3단계 : 충분히 성숙한 오니덩어리(Flock)에 고정부착하여 생식하는 고정형의 생물이 다수 출현하게 된다.The third stage: many fixed-type creatures that attach to the mature mature Flock will appear.

이렇게 하여 침강성이 양호한 덩어리(Flock)를 형성하므로써 침전조에서 상분리(相分離)시키는 정화작용을 기대할 수 있는 것이며, 여기에서 알 수가 있듯이 폐수중의 유기물질의 분해에 직접적으로 관여하는 것은 세균류(Bacteria)이며, 이들이 기질(폐수)을 분쇄시키기 위해서는 그 기질마다 독특한 효소(Enzyme)를 분비하므로서 기질을 분해하여 체세포 합성을 유발하게 됨도 역시 주지의 사실에 속하는 것이다.In this way, it is possible to expect a purification effect of phase separation in the sedimentation tank by forming a flocculant having good sedimentation properties. As can be seen, it is directly involved in the decomposition of organic substances in wastewater. It is also well known that they break down the substrate and cause somatic cell synthesis by releasing a unique enzyme (Enzyme) for each substrate to crush the substrate (wastewater).

본 발명은 이상에서 설명한 바와 같이 폐수중의 유기물질을 분해할 수 있는 효소물질과 미량원소(MgSO4, FeSo4, CaCl2)를 공급하므로서 균류의 번식을 활성화 함과 동시에 오염수질(폐수)을보다 효과적으로 정화시킬 수 있는 방법을 엄도저 함에 주된 목적을 두고 있는 것이다.As described above, the present invention supplies enzymes and trace elements (MgSO 4 , FeSo 4 , CaCl 2 ) which can decompose organic substances in the wastewater, thereby activating the propagation of fungi and at the same time contaminating water quality (wastewater). Its main purpose is to limit how to cleanse more effectively.

예컨데, 종래의 폐수처리용 미생물의 영양제는 단순히 C, N, P(탄소, 질소, 인)등의 기타 무기 영양소를 부가하므로, 균류 또는 원생동물균등을 왕성하게 증식될 수 있도록 유도하고 있었으나, 이는 적응시간이 장시간인 관계로 인하여 시간적인 많은 손실을 초해하였던 것이며, 본발명과 비교하여 보았을 때 상당한 시간이 소요됨은 물론 특히 분해하기 어려운 유기화합물체의 색소분해 능력이 없었던 것이었고, 또한, P, H적응 범위가 좁았으며(P. H 7.0-7.5이하)독성물질의 충격에 견디기 힘드는 것이 현실이었다.For example, conventional nutrients for wastewater treatment microorganisms simply add other inorganic nutrients such as C, N, P (carbon, nitrogen, phosphorus), thereby inducing the proliferation of fungi or protozoa. Due to the long time of adaptation, it has neglected a lot of time loss, and compared with the present invention, it takes considerable time, and in particular, it was incapable of dissolving the pigments of organic compounds that are difficult to decompose. The range of H adaptation was narrow (P. H 7.0-7.5 and below) and it was difficult to withstand the impact of toxic substances.

본 발명은 종래에 이미 공지된 바 있는 영양원의 결함을 해소하거나 일부 효소를 부가하여 미생물의 생식만을 왕성하게 하기 보다는 폐수중의 분해되기 어려운 유기물질을 고농도, 다종류의 효소를 이상적으로 투여하므로서, 기질 자체를 분해 흡수할 수 있도록 해주므로서 폐수중에 함유되어 있는 COD, BOD, 색소등을(약 12시간-24시간내) 빠른 시간내에 분해 및 생성을 시키는 것이며, 고초균을 사용하므로서 P. H의 적용범위가 넓어지게 되므로(P. H 7.0-10.5)각종 악성폐수(알칼리성 폐수)의 처리를 보다 효과적으로 실현할 수 있도록한 것이다.The present invention ideally administers high concentrations of various types of enzymes in the wastewater rather than eliminating deficiencies in nutrients known in the prior art or adding some enzymes to regenerate microorganisms. By dissolving and absorbing the substrate itself, COD, BOD, and pigments contained in the waste water (within 12 to 24 hours) are rapidly decomposed and produced. The wider application range (P. H 7.0-10.5) enables more effective treatment of malignant wastewater (alkaline wastewater).

본 발명의 폐수처리용 영양조성물의 구성과 그 제조방법에 대하여 구체적으로 상세하게 설명하면 다음과 같다.Detailed description of the composition of the nutrient composition for wastewater treatment of the present invention and a method of manufacturing the same will be given below.

쌀겨에 고초균(Bacillus Substillis)을 접종 발효한 발효물과, 효모를 접종배양한 배양물에 약간의(MgSo4, FeSo4, CaCl2)등을 혼합하여서된 분발상의 제품에 관한 것이다.The present invention relates to a fermented product inoculated and fermented with Bacillus substillis in rice bran, and a powdery product obtained by mixing some (MgSo 4 , FeSo 4 , CaCl 2 ), etc., with a culture inoculated with yeast.

이상과 같은 본발명을 공정별로 상세히 설명하면 다음과 같다.The present invention as described above will be described in detail by process.

제1공정 : (쌀겨 발효물의 제조) : 쌀겨 1kg에 P, H적응력이 광범위하며 세포막 점질물질의 분비가 우수하여 양호한 미생물 오니 덩어리(Flock)를 형성할 수 있는 고초균(Bacillus Substillis)종모를 접종하고 살균수 1l를 가하여 교반 용해시킨다음 요소 15g과 5-10cc 가량의 인산(인산 : 물=1 : 4로 희석)을 투여 교반 후 30-36℃ 1일간 배양한 후, 쌀겨 5kg을 추가 투여하여 교반하면서 다시 요소 15g, 인산 10cc를 첨가 1일간 배양 한 다음 3일째 상기한 발효물을 미세하게 골고루 교반시킨 후 2일간 더 발효시킨 다음 건조 상자에 옮겨 2일간 건조 시킨다.Step 1: (Production of fermented rice bran): Inoculate and sterilize Bacillus substillis seed, which has a wide range of P and H adaptability to 1 kg of rice bran, and has excellent secretion of cell membrane viscous material, which can form a good microbial sludge mass. 1 l of water was added to dissolve and stirred, and then 15 g of urea and 5-10 cc of phosphoric acid (phosphate: diluted with water = 1: 4) were administered. After stirring, the cells were incubated at 30-36 ° C. for 1 day. After 15g of urea and 10cc of phosphoric acid were added, the fermented product was finely evenly stirred on the third day, fermented for 2 more days, and then transferred to a drying box for 2 days.

제2공정 : 쌀겨 1kg에 P. H 적응력이 광범위하며 또한 고농도 염류에도 적응력이 우수하고 세포막 점질 물질의 분비가 우수하여 양호한 미생물 오니덩어리(Flock)를 형성할 수 있는 납두균(Bacillus Notto)종모를 접종하고 살균수 1l를 가하여 교반 용해 시킨 다음 요소 15g과 5-10cc 가량의 인산(인산 : 물=1 : 4로 희석)을 투여 교반후 30-36℃로 1일간 배양한 후, 쌀겨 5kg을 추가 투여하여 교반하면서 다시 요소 15g, 인산 10cc를 첨가 1일간 배양한 다음 3일째 상기한 발효물을 미세하게 골고루 교반시킨후 2일간 더 발효시킨 다음 건조 상자에 옮겨 2일간 건조시킨다.Step 2: 1kg of rice bran is inoculated with Bacillus Notto seed, which has a wide adaptability to P. H and has excellent adaptability to high concentration salts and excellent secretion of cell membrane viscous material to form a good microbial flock. 1l of sterilized water was added to dissolve the solution, and then 15g of urea and 5-10cc of phosphoric acid (phosphate: diluted in water = 1: 4) were administered. After stirring, the cells were incubated at 30-36 ° C for 1 day, followed by 5kg of rice bran. 15 g of urea and 10 cc of phosphoric acid were added again while stirring, followed by incubation for 3 days. The fermented product was stirred evenly on the third day and further fermented for 2 days, and then transferred to a drying box for 2 days.

제3공정 : 쌀겨 1kg에 효모균류를 접종하고 살균수 1l를 가하여 교반 시킨다음 규체증식을 보다 높이기 위해서 5-10cc 가량의 인산(인산 : 물=1 : 4로 희석)을 투여 교반한 후 쌀겨 5kg을 추가 투여 교반시킨 다음 30-36℃로 3일간 배양한 후 건조상에 옮겨 2일간 건조 시킨다.Step 3: Inoculate yeast fungus to 1kg of rice bran, add 1l of sterilized water and stir, add 5-10cc of phosphoric acid (phosphate: dilute with water = 1: 4) and increase rice broth 5 kg of the solution was further stirred and incubated at 30-36 ° C. for 3 days, and then transferred to a dry phase and dried for 2 days.

제4공정 : 제1공정의 배양물 50g과 제2공정의 배양물 25%, 제3공정의 배양물 25%에 미량원소(MgSo4, 0.1%, (FeSo4) 0.1%, (CaCl2) 0.4%(중량단위 %)로 혼합 분쇄한 후 포장 제품화 한다.4th step: 50g of the culture of the first step, 25% of the culture of the second step, 25% of the culture of the third step, trace elements (MgSo 4 , 0.1%, (FeSo 4 ) 0.1%, (CaCl 2 ) Mix and pulverize to 0.4% (weight unit%) and package it.

이상과 같이 각 공정별로 설명한 본 발명의 영양조성물을 각종 공업폐수(활성오니처리법)에 투여하게 되면, 상기한 제1공정에서 배양된 고초균(Bacillus Sudstillis)은 P. H 적응범위가 넓어(P.H 7.0-10.5) 특히 알카리성 폐수에 적응력이 우수할 뿐 아니라, 세포막 점질물질을 다량 분비하게 되므로서 양호한 미생물오니덩어리(Flock)를 형성시켜 상분리가 쉽게 이루어지게 되며, 입자상 유기물질의 흡착을 유도하므로 폐수중의 SS(부유물질) 제거에 탁원한 효과를 갖게 되는 것이다.As described above, when the nutrient composition of the present invention described for each step is administered to various industrial wastewaters (Active sludge treatment method), Bacillus Sudstillis cultured in the first step has a wide range of P. H adaptability (PH 7.0). -10.5) In particular, it is excellent in adaptability to alkaline wastewater, and it secretes large amount of cell membrane viscous material to form good microbial flock, which makes phase separation easier and induces adsorption of particulate organic material. It will have a turbid effect on the removal of SS (floating matter).

또한 고초균을 쌀겨 배지에서 각종 효소 특히 프로테아제(Protease)가 다량 분비케 되므로 폐수내의 고분자 유기물질인 합성수지계 폐수를 효과적으로 분해할 수 있는 것이다.In addition, since various enzymes, especially protease, are secreted from Bacillus bran medium, it is possible to effectively decompose synthetic resin wastewater, which is a polymer organic material in the wastewater.

그리고 제2공정에서 배양된 납두균(Bacillus Natto)은 이미 주지된 사실과 같이 고농도 염류에 적응력이 매우 우수하므로 (염류농도 5%)수산물 가공폐수의 처리에 탁원한 효과를 기대할 수 있는 것이다.And Bacillus Natto cultured in the second process (Bacillus Natto) is already well known to adapt to high concentrations of salts (since salt concentration 5%) can be expected to have a turbid effect in the treatment of marine product wastewater.

상기한 공정에 요소를 첨가 발효시키므로써 결핍된 질소를 보충할 수 있으며 효소의 역가를 높일 수 있는데, 여기서 나타나는 프로테아제(Protease)는 폐수중 단백질 계통의 유기물질을 응결, 침전시키는 중요한 효과를 갖게 되는 것이다.By adding and fermenting urea to the above process, it is possible to replenish the depleted nitrogen and increase the enzyme titer. The protease, which appears here, has an important effect of condensation and precipitation of organic substances in the protein system in wastewater. will be.

제3공정에서 배양된 효모는 상기한 제1공정에서 배양된 고초균과 염색폐수중에 투여하여 상존 시켰을 때 임의성에서 색소의 제거효과를 갖게 되는데, 염색폐수 1l에 본발명의 제품 1g을 투여한후 온도는 약 28℃-30℃를 유지한 상태로 12-24시간 정도에서 색소제거 작용이 나타나게 되는 것이다.Yeast cultivated in the third step has the effect of removing the pigment in the randomness when it is present in the subtilis cultured in the first step and in the dye wastewater, and the temperature after administration of 1 g of the product of the present invention to 1 l of the dye wastewater Is the pigment removal action will appear in about 12-24 hours while maintaining about 28 ℃ -30 ℃.

그리고 제4공정에서 첨가되는(MgSo4, FeSo4, CaCl2)는 미생물이 성장하는데 필요로 하는 미량원소로 첨가되는 것이다.And (MgSo 4 , FeSo 4 , CaCl 2 ) added in the fourth step is added as a trace element required for the growth of microorganisms.

Claims (1)

쌀겨에 고초균(Bacillus Substillis)을 접종발효한 발효물과, 쌀겨에 납두균(Bacillus Natto)을 접종 발효한 발효물에 효모 배양물을 혼합하고 미량의 MgSo4, FeSo4, CaCl2을 첨가 교반한후 분쇄하여서 포장 제품화하는 것을 특징으로 하는 폐수처리용 활성오니 영양제조성물 제조방법.The yeast culture was mixed with the fermentation product inoculated with Bacillus substillis in rice bran and the fermentation product inoculated with Bacillus Natto in rice bran, followed by stirring with addition of trace amounts of MgSo 4 , FeSo 4 , CaCl 2 . Method for producing activated sludge nutritional composition for wastewater treatment, characterized by pulverizing and packaging products.
KR1019850003029A 1985-05-03 1985-05-03 Preparation for flock accelator of waste water KR880000583B1 (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006082264A1 (en) * 2005-01-28 2006-08-10 Universidad De Sevilla Method of obtaining biostimulants from agro-industrial residues
CN102391027A (en) * 2011-08-03 2012-03-28 福建省亚热带植物研究所 Conditioner for fermenting organic fertilizer from sludge and manufacturing and using methods thereof
CN105399218A (en) * 2015-12-18 2016-03-16 仝荣 Sewage treatment method of biological bacteria

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006082264A1 (en) * 2005-01-28 2006-08-10 Universidad De Sevilla Method of obtaining biostimulants from agro-industrial residues
ES2259542A1 (en) * 2005-01-28 2006-10-01 Universidad De Sevilla Method of obtaining biostimulants from agro-industrial residues
CN102391027A (en) * 2011-08-03 2012-03-28 福建省亚热带植物研究所 Conditioner for fermenting organic fertilizer from sludge and manufacturing and using methods thereof
CN105399218A (en) * 2015-12-18 2016-03-16 仝荣 Sewage treatment method of biological bacteria

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