CN113403238A - Method for industrially, continuously and efficiently producing nitrifying bacteria agent - Google Patents
Method for industrially, continuously and efficiently producing nitrifying bacteria agent Download PDFInfo
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Abstract
The invention discloses a method for industrially, continuously and efficiently producing a nitrifying bacteria agent, which is characterized in that in the process of treating high-ammonia nitrogen industrial wastewater by a high-efficiency nitration reactor, residual sludge is periodically added and the nitrifying bacteria agent is discharged, and nitrifying bacteria are screened, cultured and enriched on line, so that the nitrifying bacteria agent is industrially, continuously and efficiently produced. The invention can realize simple, economic and efficient continuous production of the nitrifying agent, and the nitrifying agent obtained by production has low cost, stable quality, high nitrification performance and good environmental compatibility, thereby being beneficial to the industrialized popularization and application of the nitrifying agent biological enhancement technology in the field of sewage denitrification.
Description
Technical Field
The invention relates to the field of nitrobacteria production, in particular to a method for industrially, continuously and efficiently producing nitrobacteria, and mainly aims to industrially, continuously and efficiently produce the nitrobacteria by periodically adding excess sludge and discharging the nitrobacteria, and quickly and economically screening, culturing and enriching nitrobacteria on line in the process of treating high-ammonia nitrogen industrial wastewater by a high-efficiency nitration reactor.
Background
The nitrifying bacteria are the core of the biological denitrification technology, and the key for removing ammonia nitrogen pollutants in the water body is to enhance the catabolism capability of the nitrifying bacteria. Although nitrifying bacteria exist widely in nature, the nitrifying bacteria are chemoautotrophic bacteria, have low energy utilization rate and slow growth, and are sensitive to the change of environmental factors, so that the industrial application of the nitrifying bacteria still has some difficulties. Therefore, the aim of improving the denitrification efficiency of the system, maintaining the share of effective functional bacteria in the sewage treatment system and enhancing the adaptability of the effective functional bacteria to the environment is very important, in particular to a biological treatment device for nitrifying high ammonia nitrogen industrial wastewater.
A biological strengthening technology which is used for adding a nitrifying bacteria agent into a sewage treatment system in a targeted manner, improving the content of nitrifying bacteria and strengthening the nitrification performance is an effective method for improving the biological denitrification effect. However, the commercial autotrophic nitrifying bacteria have high price and uneven quality, so that the large-scale industrial application of the products is not facilitated. Therefore, the method for producing the nitrifying bacteria agent efficiently, economically and stably is an effective way for applying the biological strengthening technology to sewage denitrification. CN106434412B invents a nitrifying bacteria continuous production method and production equipment thereof, can realize the continuous culture and the high-efficient enrichment of nitrifying bacteria, but the method only relies on the self-proliferation of nitrifying bacteria to improve the bacterial quantity, the production cycle of products is longer, and the production cost of the microbial inoculum is increased by independently building a nitrifying bacteria production line, equipment, medicament and production process. Therefore, aiming at the application scene of the nitrifying bacteria agent, a simple, economic and efficient industrial nitrifying bacteria agent production method is urgently needed in the market.
Disclosure of Invention
Aiming at the problems of long production period, low efficiency and high production cost of the existing nitrobacteria production method, the invention provides a simple, economic and efficient industrial continuous production method of nitrobacteria by combining the background technology.
The technical scheme of the invention is as follows:
the invention provides a method for realizing industrial continuous and efficient production of a nitrifying agent, which comprises the steps of adding excess sludge into a high-efficiency nitration reactor in the process of treating high-ammonia nitrogen industrial wastewater by the high-efficiency nitration reactor, realizing screening, culturing and enrichment of nitrifying bacteria on line along with the operation of the reactor, discharging the nitrifying agent from the high-efficiency nitration reactor after the enrichment of the nitrifying bacteria is finished, adding the excess sludge again after the nitrifying agent is discharged, circulating in sequence, and realizing continuous and efficient production of the nitrifying agent through a periodic adding and discharging strategy.
The excess sludge is excess sludge in a biological treatment section of a sewage plant, the organic matter proportion VSS/SS of the excess sludge is 30-70%, the relative abundance of nitrobacteria in microbial components is 0.3-5.0%, and the sources of the excess sludge comprise a domestic sewage treatment plant and an industrial sewage treatment plant. The excess sludge provides a bacterial source for the production of the nitrifying agent, and meanwhile, the excess sludge can be decomposed in the production process of the nitrifying agent to provide nutrient substances (micromolecular organic matters, inorganic salts and trace elements) necessary for the growth and metabolism of microorganisms for the high-efficiency nitration reactor, so that the cost of adding nutrient solution in the nitration treatment process of the high-ammonia nitrogen industrial wastewater is saved, and the performance and the stability of the reactor are improved. The adding amount of the excess sludge is 5% -50% of the total sludge in the reactor.
In the existing biological nitrification treatment process of high ammonia nitrogen industrial wastewater, nitrifying bacteria added in excess sludge are easier to be screened, cultured and enriched, thereby realizing the high-efficiency production of nitrifying bacteria. And the processing time of the online screening, culturing and enriching of the nitrifying bacteria is 3-20 d.
After the nitrifying bacteria are screened, cultured and enriched, discharging the nitrifying bacteria agent from the reactor; the discharge amount of the nitrifying bacteria agent is 5% -50% of the effective volume of the reactor.
The discharged nitrifying bacteria agent SS is 2000-6000 mg/L, the organic matter ratio VSS/SS is 50% -100%, the sludge index SVI is 30-100 ml/g, the sludge nitrification load is 0.5-2.0 kg-N/kg.d, and the relative abundance of nitrifying bacteria in the microorganism components is 10% -60%.
The high ammonia nitrogen industrial wastewater related by the method is industrial wastewater containing 100-3000 mg-N/L ammonia nitrogen, and nutrient solution can be optionally supplemented according to needs.
The high-efficiency nitration reactor involved in the method is a biological reactor capable of efficiently nitrating and treating high ammonia nitrogen industrial wastewaterThe treatment device has the nitrated ammonia nitrogen removal load of 1.5-8.0 kg-N/m3D. The reactor has the operating temperature of 15-35 ℃, the pH value of 7.0-9.0 and the DO of 0.5-9.0 mg/L.
The invention has the advantages that: compared with the traditional nitrifying agent production device and a production method for producing the agent, the method disclosed by the invention has the advantages that the continuous production of the online nitrifying agent is realized in the high-ammonia-nitrogen industrial wastewater treatment process of a sewage plant, the independent construction of the agent production device is not needed, the equipment, agent and operation cost investment in the traditional agent production process is saved, and the production cost of the nitrifying agent is low. The traditional nitrobacteria production method increases the bacterial quantity by means of nitrobacteria self-proliferation, but the nitrobacteria as chemoautotrophic bacteria grow slowly, and the nitrification activity is easily influenced by environmental factors, so that the production period of the nitrobacteria is long and the nitrification performance is unstable. Compared with the prior art, the method adopts the periodic feeding and discharging strategy, continuously and efficiently screens, cultures and enriches the nitrifying bacteria from the residual sludge on line under the condition of not influencing normal sewage treatment, the production process is simpler and quicker, the produced nitrifying bacteria agent is derived from actual activated sludge, the environmental adaptation is strong, the nitrification performance is high and stable, and the method is suitable for industrial application. In addition, the method utilizes the residual sludge of the sewage plant and the actual high ammonia nitrogen industrial wastewater as raw materials to produce the nitrifying bacteria agent, realizes the resource utilization of waste, and is more environment-friendly compared with the traditional nitrifying bacteria agent production method. Meanwhile, in the process of producing the nitrifying agent, the added endogenous nutrient substances released by decomposing the excess sludge can be used for the growth and metabolism of microorganisms in the reactor, so that the adding amount of the nutrient solution in the process of efficiently nitrifying and treating the high-ammonia nitrogen industrial wastewater by the sewage plant is reduced, and the sewage treatment cost of the sewage plant is reduced.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the present invention will be further described in detail with reference to the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention. And are not intended to limit the invention.
Example 1
By means of a reactorThe high-efficiency nitration reactor is operated in a small trial in a laboratory, the reactor type is MBR, the volume is 5L, and the removal load of nitrated ammonia nitrogen is 5.9-6.1 kg-N/m3D, the operation temperature is 25-35 ℃, the pH value is 7.5-8.0, and DO is 2.0-5.0 mg/L. The water inlet of the reactor is artificial water, contains carbon source, nitrogen source, growth factor, inorganic salt and trace elements, and has balanced nutrition and the ammonia nitrogen concentration of 1500 mg-N/L. The added residual sludge comes from a secondary sedimentation tank of a biochemical section of a municipal sewage plant, the VSS/SS is 50-60%, and the relative abundance of nitrobacteria in microbial components is 0.5-1.0%. Adding residual sludge into the high-efficiency nitration reactor, wherein the adding amount is 40% of the total sludge amount in the reactor. The feed water is adjusted to only contain 1500 mg-N/L ammonia nitrogen and a proper amount of sodium bicarbonate, the nitrifying bacteria agent is discharged after the reactor continuously runs for 15 days, and the discharge amount is 50 percent of the effective volume of the reactor. And (3) adding residual sludge accounting for 40 percent of the total sludge amount again after sludge discharge, and sequentially circulating, periodically adding and discharging to continuously produce the nitrifying bacteria agent. And monitoring the running state of the reactor, determining the quality of the nitrobacteria produced in each batch, and properly adjusting the production strategy according to the quality of each batch of the nitrobacteria to ensure the product quality. In the production process of the microbial inoculum, the removal load of nitrated ammonia nitrogen of the high-efficiency nitration reactor is 5.2-6.1 kg-N/m3D, the specific production scheme and the quality of the produced microbial inoculum are shown in Table 1.
Table 1 experimental data for example 1
Example 2
The reactor is a pilot-scale operation high-efficiency nitration reactor of a certain coking wastewater plant, the type of the reactor is MBR, the volume is 500L, and the removal load of nitrated ammonia nitrogen is 3.9-4.8 kg-N/m3D, the operation temperature is 15-28 ℃, the pH value is 7.0-8.0, and DO is 0.5-3.0 mg/L. The water inlet of the reactor is the effluent of the anaerobic treatment of the coking wastewater, nutrient solution and alkalinity are added, nutrition is balanced, and the concentration of ammonia nitrogen is 800-1000 mg-N/L. The added residual sludge comes from a biochemical-stage secondary sedimentation tank of a main stream treatment process of the coking wastewater plant, the VSS/SS is 35-50%, and the relative abundance of nitrobacteria in microbial components is 1.2-3.0%. Adding into a high-efficiency nitration reactorThe adding amount of the residual sludge is 30 percent of the total sludge in the reactor. The nutrient solution is not supplemented any more when the water is fed, the nitrifying bacteria agent is discharged after the reactor is continuously operated for 12 days, and the discharge amount is 30 percent of the effective volume of the reactor. And (3) adding the excess sludge accounting for 30 percent of the total sludge amount again after sludge discharge, and sequentially circulating, periodically adding and discharging to continuously produce the nitrifying bacteria agent. And monitoring the running state of the reactor, determining the quality of the nitrobacteria produced in each batch, and properly adjusting the production strategy according to the quality of each batch of the nitrobacteria to ensure the product quality. In the production process of the microbial inoculum, the removal load of nitrated ammonia nitrogen of the high-efficiency nitration reactor is 3.0-4.8 kg-N/m3D, the specific production scheme and the quality of the produced microbial inoculum are shown in Table 2.
Table 2 experimental data for example 2
Example 3
The reactor is a high-efficiency nitration reactor which is operated in a certain nitrogenous fertilizer plant, the type of the reactor is SBR, and the volume of the reactor is 1 m3The removal load of the nitrified ammonia nitrogen is 2.1-3.0 kg-N/m3D, the operation temperature is 15-25 ℃, the pH value is 7.5-9.0, and DO is 4.0-9.0 mg/L. The water inlet of the reactor is the high ammonia nitrogen actual wastewater of the nitrogen fertilizer plant supplemented with nutrient solution and alkalinity, and the ammonia nitrogen concentration is 300-500 mg/L. The added residual sludge comes from a secondary sedimentation tank of a biochemical section of a main flow treatment process of the nitrogen fertilizer plant, the VSS/SS is 40-50%, and the relative abundance of nitrobacteria in microbial components is 2.2-4.3%. Adding residual sludge into the high-efficiency nitration reactor, wherein the adding amount is 10% of the total sludge amount in the reactor. And (3) no nutrient solution is supplemented to the inflow water, the nitrifying bacteria agent is discharged after the reactor is continuously operated for 7 days, and the discharge amount is 20% of the effective volume of the reactor. And (3) adding the residual sludge accounting for 20 percent of the total sludge amount again after sludge discharge, and circulating, periodically adding and discharging in sequence to continuously produce the nitrifying bacteria agent. And monitoring the running state of the reactor, determining the quality of the nitrobacteria produced in each batch, and properly adjusting the production strategy according to the quality of each batch of the nitrobacteria to ensure the product quality. In the production process of the microbial inoculum, the removal load of nitrated ammonia nitrogen of the high-efficiency nitration reactor is 1.5-3.0 kg-N/m3D, specific production protocol and productsThe quality of the microbial inoculum is shown in Table 3.
Table 3 experimental data for example 3
The continuous and efficient production of the nitrifying agent can be realized by adopting the method under the conditions of different efficient nitration reactor types and different high ammonia nitrogen wastewater quality, the quality of the produced nitrifying agent is stable, and the feasibility and universality of the method for producing the nitrifying agent are proved.
The above-mentioned embodiments are only preferred embodiments of the present invention, and not intended to limit the scope of the present invention, and various modifications and applications made according to the above-mentioned embodiments are within the scope of the present invention.
Claims (6)
1. The method for industrially, continuously and efficiently producing the nitrifying bacteria agent is characterized by comprising the following steps of synchronously carrying out the following steps in the process of treating high ammonia nitrogen industrial wastewater by using a high-efficiency nitration reactor:
(1) adding excess sludge into the reactor;
(2) screening, culturing and enriching nitrobacteria on line from the added excess sludge;
(3) discharging the nitrifying agent from the reactor;
and (4) after the step (3) is finished, adding excess sludge again, and sequentially and circularly performing the steps (1) - (3).
2. The method as claimed in claim 1, wherein in the excess sludge added in the step (1), the ratio of organic matters to VSS/SS is 30% -70%, and the relative abundance of nitrifying bacteria in the microbial components is 0.3% -5.0%; the sources of the excess sludge comprise a domestic sewage treatment plant and an industrial wastewater treatment plant, and the adding amount of the excess sludge is 5-50% of the total sludge in the reactor.
3. The method of claim 1, wherein the treatment time of the step (2) is 3-20 d.
4. The method according to claim 1, wherein the amount of the nitrifying bacteria discharged in the step (3) is 5 to 50 percent of the effective volume of the reactor.
5. The method of claim 1, wherein the feed water to the high efficiency nitration reactor comprises industrial wastewater containing 100-3000 mg-N/L ammonia nitrogen, and further optionally comprises additional nutrient solution.
6. The method according to claim 1, wherein the nitrated ammonia nitrogen removal load of the high-efficiency nitration reactor is 1.5-8.0 kg-N/m3D, the operating conditions comprise the temperature of 15-35 ℃, the pH value of 7.0-9.0 and the DO of 0.5-9.0 mg/L.
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| CN115927125A (en) * | 2023-02-15 | 2023-04-07 | 知和环保科技有限公司 | Production process of high-load short-cut nitrifying bacteria agent and application method of bacteria agent |
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