CN101225405A - Method for producing microbial flocculant and method of use thereof - Google Patents
Method for producing microbial flocculant and method of use thereof Download PDFInfo
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- CN101225405A CN101225405A CNA2008100173518A CN200810017351A CN101225405A CN 101225405 A CN101225405 A CN 101225405A CN A2008100173518 A CNA2008100173518 A CN A2008100173518A CN 200810017351 A CN200810017351 A CN 200810017351A CN 101225405 A CN101225405 A CN 101225405A
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- microbial flocculant
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Abstract
The invention discloses a preparation method for microbial flocculant, which adopts starch waste as carbon source and complex nitrogen source to produce flocculant through fermentation. The culture process comprises following steps: pre-fermenting starch wastewater with the COD of 12000 to 15000 mg/L though aeration; adding 200-500mg/L nitrogen source, 300-800mg/L phosphorus and 10-25mg/L magnesium sulphate; adjusting the pH value to 6.0 to 8.0; introducing klebsiella sp seed liquid after autoclaving; aerating using shaking table; cultivating 24 to 44h to obtain crude product of the flocculant. The microbial flocculant produced using the method has the advantages of high flocculation efficiency and less adding quantity. The invention also discloses an application method for the microbial flocculant, which is characterized in combining the microbial flocculant with calcium ion, aluminum ion and ferric ion; wherein, the adding concentration of the calcium ion is 72 to 180mg/L, while the aluminum ion and ferric ion are 0.2 to 0.6mg/L. When the microbial flocculant is used for water treatment, water treatment cost can be reduced.
Description
Technical field
The present invention relates to a kind of flocculation agent, particularly a kind of method of producing flocculation agent with microbial fermentation; The invention still further relates to the using method of this flocculation agent.
Background technology
1. the molecular structure characteristics of microbial flocculant: microbial flocculant is a kind of of biological flocculant, it by microorganisms, can make in the liquid aggegation, the sedimentary special natural high moleculer eompounds such as solid suspended particle, somatic cells and colloidal particle that are difficult for degraded, be to utilize biotechnology, by microbial fermentation, extraction, refining a kind of novel, the water conditioner efficiently that obtains, mainly form by polysaccharide, glycoprotein, Mierocrystalline cellulose, DNA, protein and fat etc. with biological readily degradable and environmental safety.
2. produce two key issues of microbial flocculant: one, acquisition has the bacterial strain of high yield microbial flocculant: the synthetic strictness of microbial flocculant is subjected to genetic control, and also found the gene of produce flocculant in recent years, generally because of the gene segment difference of produce flocculant, institute's produce flocculant molecular structure is then different.Since the 1950's research document so far, nearly more than 25 kinds, they are distributed among bacterium, actinomycetes, mould and the yeast microorganism of the produce flocculant of having found so far.Having obtained efficiently, bacterial classification can guarantee high produce flocculant.They are two years old, fermentation condition: comprise the generation microbial flocculant culture of strains condition of pH value, culture temperature, air flow, incubation time, stir speed (S.S.), external ions kind and ionic strength etc. of composition, the substratum of substratum, should some difference for these conditions of different bacterial strains.
3. the problem that exists in the present microbial flocculant performance history: biological polymer receives much attention as flocculation agent in recent years, the polyose flocculation agent that especially stable height and flocculation activity are high.The existing problem of microbial flocculant is the production cost height at present, is difficult to large-scale industrial production and use.Its major cause is that required pharmaceutical chemicals usage quantity is big during purification owing to the required substratum price of microorganism growth height.
4. produce the present Research of flocculation agent about Klebsiella sp bacterium: Klebsiellapneumoniae can produce polysaccharose substance, as far back as Klebsiella sp in 1999 as producing the separated and understanding of microbial flocculant Pseudomonas.Subsequently, the structure of the polysaccharose substance of Klebsiella generation is identified.There is report to utilize Klebsiella mobilis fermented diary prod enterprise wastewater to produce flocculation agent.Bibliographical information in 2007 is based on the mutation of redox potential sharp separation Klebsiella pneumoniae, to improve the output capacity of 1.3-propylene glycol.Also do not see at present the report that utilizes Klebsiella pneumoniae fermentation starch wastewater to produce flocculation agent.
5. about the medium optimization present Research of microorganisms producing flocculation agent: microbial flocculant fermention medium carbon source glucose 10-20g/L mostly at present, mixtures such as nitrogenous source yeast extract paste (0.5g/L), urea (0.5g/L), peptone (1.0g/L) or ammonium sulfate, phosphorus source K
2HPO
4(5g/L) and KH
2PO
4Mixture (2g/L).The flocculation agent price can be in any more, mainly is to have used the expensive reagent of these prices in the substratum.Have in recent years and use discarded carbon source through fermentation to produce flocculation agent, the flocculation agent cost is decreased, but utilize microbiological treatment water, add the flocculation agent expense still between 1-1.5 unit/ton water, and the corresponding expense of inorganic flocculating agent treating water is in 0.1-0.3 unit/ton water scope.Therefore, although microbial flocculant is green water conditioner, safety non-toxic does not possess price competitiveness, does not obtain scale operation and use at present.
6. about the present Research of microbial flocculant application method: microbial flocculant price height,, in applied research, find inorganic cation, especially Ca in order to obtain high flocculation agent efficient and few dosage
2+Can be as the coagulant aids of microbial flocculant.Under the situation that does not influence flocculating effect, can be with Ca
2+With a spot of Fe
3+(0.3ppm is following) and Al
3+(0.3ppm following) composite use does not appear in the newspapers with the research of the usage quantity of obvious reduction microbial flocculant.
Summary of the invention
The technical problem to be solved in the present invention is: overcome the deficiencies in the prior art, a kind of production method of microbial flocculant is provided, the microbial flocculant of producing with this method can obtain high flocculation efficiency and few dosage.
For solving the problems of the technologies described above, the technical scheme of technical solution problem of the present invention is such:
With starch wastewater as carbon source, utilize compound nitrogen source fermentative production flocculation agent, this culture process is: COD is after the starch wastewater of 1.2-1.5 ten thousand mg/L ferments in advance through aeration, add the nitrogenous source of 200-500mg/L, the phosphorus of 300-800mg/L, the sal epsom of 10-25mg/L, transfer to pH6.0-8.0, insert the Klebsiella seed liquor behind the autoclaving, through biological shaking table aeration, results flocculation agent crude product behind cultivation 24～44h.
Described nitrogenous source is the compound nitrogen source of ammonium sulfate and urea.
The pre-fermentation of described aeration is: in the open container, use distiller's yeast aerobic fermentation 3-10h under the room temperature.
The optical density(OD) of described Klebsiella seed liquor is 1.5-1.8.
Described Klebsiella seed liquor inoculum size is: the described Klebsiella seed liquor 0.1-2ml of inoculation in the nutrient solution of 100ml production microbial flocculant.
Described biological shaking table speed is 150-170r/min.
Described culture temperature is 25-35 ℃.
Described incubation time is 24～44h.
Another technical problem that the present invention will solve is: the present invention also provides a kind of using method of microbial flocculant, can reduce the water treatment expense when carrying out water treatment with this biological flocculant.
For solving the problems of the technologies described above, the technical scheme of technical solution problem of the present invention is such:
The compound use of described biological flocculant and calcium ion, aluminum ion or iron ion, wherein calcium ion adds concentration 72-180mg/L, and aluminum ion and iron ion are all in the 0.2-0.6mg/L scope.
The invention has the beneficial effects as follows:
According to the substratum that uses in most document, the cost of fermentative production microbial flocculant liquid flocculation agent per ton crude product is about 110-150 unit, and wherein, the carbon source expense accounts for 2/3 of total cost, and the yeast extract paste expense accounts for 1/4 of total cost.Phosphorus source content can reach 7g/L in the substratum that uses in the document at present, and like this, phosphorus source expense can account for 1/5 of total cost in the fermention medium cost of production flocculation agent.
The present invention produces utilization of carbon source high concentration starch water in the substratum of flocculation agent, and nitrogenous source only utilizes ammonium sulfate and urea, and has groped the dosage of rational starch wastewater and nitrogenous source, compares with document, and the dosage in phosphorus source has reduced 30-70%.Make the microbial flocculant production cost be reduced to about 5.3% in the bibliographical information like this.This flocculation agent carries out composite use with metal ion in use, makes the dosage of flocculation agent reduce 2-4 doubly.Therefore, according to flocculation agent zymotechnique provided by the invention and using method thereof, when handling high-turbidity water or high turbidity surface water (suspended solids SS is about 5g/L in the water), the SS clearance reaches under the situation more than 95%, need add the about 0.2-0.10 of the expense unit/ton water of flocculation agent during processing.
Embodiment
The present invention utilizes an isolated strain produce flocculant bacterium from the active sludge of municipal sewage plant, this Pseudomonas Klebsiella [Klebsiella pneumoniae (Schroeter) Trevisan]; The screening and separating process of this bacterial strain is as follows: the active sludge sample is through dilution, enrichment culture, cultivate with 30 ℃ electro-heating standing-temperature cultivator culture plates, and single bacterium colony is obtained in line repeatedly.On the screening culture medium flat board, it is vigorous to grow, more sticking single bacterium colony, and the separation of repeatedly ruling again is until determining that with microscopy thalline is purer single bacterium colony.The bacterial strain that utilizes flocculating effect to test primary dcreening operation carries out multiple sieve.Obtain this bacterial strain at last, and be accredited as Klebsiella.
Embodiment
One, produces the method for biological flocculant
1. substratum: suitable pre-fermented starch wastewater (COD:1.2 ten thousand); Urea 900mg/L; Ammonium sulfate 500mg/L; Potassium primary phosphate 150mg/L; Dipotassium hydrogen phosphate 400mg/L; Sodium-chlor 100mg/L; Sal epsom 200mg/L.The thing flocculation agent that produces makes that the SS clearance can reach about 88% in the water body.
2. suitable pre-fermented starch wastewater (COD:1.3 ten thousand); Urea 400mg/L; Ammonium sulfate 1400mg/L; Potassium primary phosphate 200mg/L; Dipotassium hydrogen phosphate 350mg/L; Sodium-chlor 100mg/L; Sal epsom 180mg/L.But institute's produce flocculant makes SS clearance Da Keda about 84% in the water body.
3. suitable pre-fermented starch wastewater (COD:1.3 ten thousand); Urea 400mg/L; Ammonium sulfate 400mg/L; Potassium primary phosphate 100mg/L; Dipotassium hydrogen phosphate 450mg/L; Sodium-chlor 150mg/L; Sal epsom 200mg/L.But institute's produce flocculant makes SS clearance Da Keda about 87% in the water body.
4. suitable pre-fermented starch wastewater (COD:1.4 ten thousand); Urea: 350mg/L; Ammonium sulfate 650mg/L; Potassium primary phosphate 100mg/L; Dipotassium hydrogen phosphate 300mg/L; Sodium-chlor 100mg/L; Sal epsom 200mg/L.But institute's produce flocculant makes SS clearance Da Keda about 90% in the water body.
5. suitable pre-fermented starch wastewater (COD:1.4 ten thousand); Urea 300mg/L; Ammonium sulfate 1400mg/L; Potassium primary phosphate 150mg/L; Dipotassium hydrogen phosphate 300mg/L; Sodium-chlor 100mg/L; Sal epsom 250mg/L.But institute's produce flocculant makes SS clearance Da Keda about 90% in the water body.
6. suitable pre-fermented starch wastewater (COD:1.5 ten thousand); Urea 200mg/L; Ammonium sulfate 500mg/L; Potassium primary phosphate 100mg/L; Dipotassium hydrogen phosphate 300mg/L; Sodium-chlor 100mg/L; Sal epsom 200mg/L.But institute's produce flocculant makes SS clearance Da Keda about 89% in the water body.
Two, the using method of biological flocculant
1, contain aluminum ions solution or the compound use of 1.7mg/L iron content ion solution with (1.0ml) biological flocculant with (2ml) calcium ions solution, (4ml) 4.0mg/L of 20g/L, can handle (100ml) high turbid wastewater, flocculating effect reaches 92%.
The contrast experiment: handle (100ml) high turbid wastewater, with (1ml) biological flocculant, when not adding calcium ion, aluminum ion or iron ion, flocculating effect is 70%
2, contain aluminum ions solution or the compound use of 2.0mg/L iron content ion solution with (2ml) biological flocculant with (4ml) solution, (2ml) 3.0mg/L of the calcium ions of 15g/L, can handle (100ml) high turbid wastewater, flocculating effect reaches 90%.
The contrast experiment: handle (100ml) high turbid wastewater, with (2ml) biological flocculant, when not adding calcium ion, aluminum ion or iron ion, flocculating effect is 78%.
3, usefulness (1.5ml) amount biological flocculant and (3ml) solution of the calcium ions of 19mg/L, (3ml) 3.5mg/L contains aluminum ion or the compound use of 1.5mg/L iron content ion solution, can handle (100ml) high turbid wastewater, and flocculating effect reaches 88%.
The contrast experiment: handle (100ml) high turbid wastewater, with (3ml) biological flocculant, when not adding calcium ion, aluminum ion or iron ion, flocculating effect is 75%.
Claims (10)
1. the production method of a microbial flocculant, it is characterized in that: with starch wastewater as carbon source, with compound nitrogen source fermentative production flocculation agent, this culture process is: COD is after the starch wastewater of 1.2-1.5 ten thousand mg/L ferments in advance through aeration, add nitrogenous source, the phosphorus of 300-800mg/L and the sal epsom of 10-25mg/L of 200-500mg/L, transfer to pH6.0-8.0, insert the Klebsiella seed liquor behind the autoclaving, through biological shaking table aeration, results flocculation agent crude product behind cultivation 24～44h.
2. the production method of microbial flocculant according to claim 1, it is characterized in that: described nitrogenous source is the compound nitrogen source of ammonium sulfate and urea.
3. the production method of microbial flocculant according to claim 1 and 2, it is characterized in that: the pre-fermenting process of described aeration is: under the room temperature in the open container, with distiller's yeast aerobic fermentation 3-10h.
4. the production method of microbial flocculant according to claim 1 and 2 is characterized in that: the optical density(OD) of described Klebsiella seed liquor is for being 1.5-1.8.
5. the production method of microbial flocculant according to claim 1 and 2, it is characterized in that: described Klebsiella seed liquor inoculum size is: produce the described Klebsiella seed liquor 0.1-2ml of inoculation in the nutrient solution of microbial flocculant at 100ml.
6. the production method of microbial flocculant according to claim 1 and 2, it is characterized in that: described biological shaking table speed is 150-170r/min.
7. the production method of microbial flocculant according to claim 1 and 2, it is characterized in that: described culture temperature is 25-35 ℃.
8. the production method of microbial flocculant according to claim 1 and 2, it is characterized in that: described incubation time is 24～44h.
9. the using method of a microbial flocculant that claim 1 is produced is characterized in that: the compound use of described biological flocculant and calcium ion, aluminum ion or iron ion.
10. the using method of microbial flocculant according to claim 9, it is characterized in that: described calcium ion adds concentration 72-180mg/L, and aluminum ion and iron ion are all in the 0.2-0.6mg/L scope.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101693561B (en) * | 2009-10-13 | 2011-04-20 | 河海大学 | Method for preparing compound flocculating agent |
| CN101327975B (en) * | 2008-07-31 | 2011-09-28 | 东北大学 | Method for preparing microorganism flocculant |
| CN102876600A (en) * | 2012-09-13 | 2013-01-16 | 哈尔滨工业大学宜兴环保研究院 | High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole |
| CN103146797A (en) * | 2013-02-26 | 2013-06-12 | 重庆绿色智能技术研究院 | Compound biological flocculant and preparation method thereof |
| CN105130014A (en) * | 2015-09-14 | 2015-12-09 | 西安建筑科技大学 | Preparation method and application of cation-modified glycoprotein type microorganism flocculating agent |
| CN105349468A (en) * | 2015-12-04 | 2016-02-24 | 西南石油大学 | Microbial flocculant produced from brewery wastewater and method |
| CN105417778A (en) * | 2015-11-26 | 2016-03-23 | 南京贝克特环保科技有限公司 | Method for quickly treating excrement wastewater in large-scale dairy farm |
| CN105502852A (en) * | 2016-01-22 | 2016-04-20 | 南京贝克特环保科技有限公司 | Method for quickly treating refuse leachate of refuse incineration plant |
| CN105906070A (en) * | 2016-06-06 | 2016-08-31 | 克雷伯氏环保科技（苏州）有限公司 | Preparation method of low temperature resistance polysaccharide microbial flocculant and application thereof |
| CN106011215A (en) * | 2016-07-26 | 2016-10-12 | 日照清水源生物科技有限公司 | Preparation method of microbial flocculant for purifying aquaculture seawater |
| CN107794238A (en) * | 2017-11-13 | 2018-03-13 | 中国科学院成都生物研究所 | The bacterial strains of BFX 01 of high yield biological flocculant and the biological flocculant of acquisition |
| CN109592795A (en) * | 2018-12-26 | 2019-04-09 | 金华市呗力水产养殖技术有限公司 | Disinfectant and preparation method thereof for aquaculture |
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2008
- 2008-01-21 CN CNA2008100173518A patent/CN101225405A/en active Pending
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101327975B (en) * | 2008-07-31 | 2011-09-28 | 东北大学 | Method for preparing microorganism flocculant |
| CN101693561B (en) * | 2009-10-13 | 2011-04-20 | 河海大学 | Method for preparing compound flocculating agent |
| CN102876600A (en) * | 2012-09-13 | 2013-01-16 | 哈尔滨工业大学宜兴环保研究院 | High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium to treatment of sulfamethoxazole |
| CN102876600B (en) * | 2012-09-13 | 2013-08-07 | 哈尔滨工业大学宜兴环保研究院 | High-efficiency bioflocculant producing bacterium, screening method thereof and application of high-efficiency bioflocculant producing bacterium in treatment of sulfamethoxazole |
| CN103146797A (en) * | 2013-02-26 | 2013-06-12 | 重庆绿色智能技术研究院 | Compound biological flocculant and preparation method thereof |
| CN103146797B (en) * | 2013-02-26 | 2015-04-15 | 中国科学院重庆绿色智能技术研究院 | Compound biological flocculant and preparation method thereof |
| CN105130014A (en) * | 2015-09-14 | 2015-12-09 | 西安建筑科技大学 | Preparation method and application of cation-modified glycoprotein type microorganism flocculating agent |
| CN105417778A (en) * | 2015-11-26 | 2016-03-23 | 南京贝克特环保科技有限公司 | Method for quickly treating excrement wastewater in large-scale dairy farm |
| CN105417778B (en) * | 2015-11-26 | 2018-05-25 | 南京贝克特环保科技有限公司 | A kind of method of quick treatment scale cattle farm waste waste water |
| CN105349468A (en) * | 2015-12-04 | 2016-02-24 | 西南石油大学 | Microbial flocculant produced from brewery wastewater and method |
| CN105502852A (en) * | 2016-01-22 | 2016-04-20 | 南京贝克特环保科技有限公司 | Method for quickly treating refuse leachate of refuse incineration plant |
| CN105502852B (en) * | 2016-01-22 | 2018-06-19 | 南京贝克特环保科技有限公司 | A kind of method of quick processing garbage burning factory landfill leachate |
| CN105906070A (en) * | 2016-06-06 | 2016-08-31 | 克雷伯氏环保科技（苏州）有限公司 | Preparation method of low temperature resistance polysaccharide microbial flocculant and application thereof |
| CN106011215A (en) * | 2016-07-26 | 2016-10-12 | 日照清水源生物科技有限公司 | Preparation method of microbial flocculant for purifying aquaculture seawater |
| CN106011215B (en) * | 2016-07-26 | 2019-09-13 | 青岛耀东生物工程有限公司 | A kind of breeding seawater purification preparation method of microbial flocculant |
| CN107794238A (en) * | 2017-11-13 | 2018-03-13 | 中国科学院成都生物研究所 | The bacterial strains of BFX 01 of high yield biological flocculant and the biological flocculant of acquisition |
| CN109592795A (en) * | 2018-12-26 | 2019-04-09 | 金华市呗力水产养殖技术有限公司 | Disinfectant and preparation method thereof for aquaculture |
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