CN104925957B - Composite water restoration agent containing bacillus - Google Patents
Composite water restoration agent containing bacillus Download PDFInfo
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- CN104925957B CN104925957B CN201510104650.5A CN201510104650A CN104925957B CN 104925957 B CN104925957 B CN 104925957B CN 201510104650 A CN201510104650 A CN 201510104650A CN 104925957 B CN104925957 B CN 104925957B
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- bacillus
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Abstract
The invention relates to a composite water restoration agent containing bacillus. The restoration agent comprises the components of, by weight, 1-25 parts of bacillus subtilis, 1-25 parts of bacillus licheniformis, 1-25 parts of bacillus amyloliquefaciens, 1-25 parts of bacillus pumilus, 10-30 parts of sodium hydrogen carbonate, 10-30 parts of sodium percarbonate, 10-30 parts of calcium carbonate, 5-20 parts of citric acid, and 5-20 parts of polymerized aluminum sulfate. The water restoration agent has the following beneficial effects: the four bacillus medicinal viable cell powders and the compounds such as an oxygenating agent are combined according to a certain ratio, such that the multiple active products and the compounds provide a synergistic effect; with the composite water restoration agent, the cost is low, and production process is simple; with the water restoration agent, culture water COD, nitrite value and ammonia nitrogen value can be effectively reduced, and a significant restoration function is provided for a polluted water body.
Description
Technical field
The invention belongs to field of environment protection, be specifically related to a kind of compound water body renovation agent containing bacillus cereus.
Background technology
Water is that human society is depended on for existence the most indispensable natural resources, is also the basic resource of social sustainable development.In recent years
Come, along with accelerated development and people's environmental consciousness thin of industry, exacerbate the transition consumption of world water resources, and all kinds of
Water body is polluted in various degree, becomes restriction China's economic development and affects the bottleneck of national health.Therefore, fresh water money is solved
The problem of source sustainable use is the most very urgent.Microbial environment repairing method is a kind of green environment improvement side developed this year
Method, has repair time short, easy and simple to handle, the features such as expense is low, environmental friendliness.Bacillus cereus is that one utilizes microorganism to repair
The conventional probiotic bacteria of multiple water body environment, mainly comprises bacillus amyloliquefaciens, and the type bacterial strain has improvement water quality, to cultivation water
In body and bed mud, ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, COD equal size have effect of substantially clearing up；Bacillus pumilus, the type bacterium
Strain has stronger extracellular protease, lipase, amylase activity, has inhibition widely to vibrio, and does not have haemolysis
Activity, is a kind of microorganism formulation preventing and treating Aquatic product bacterial disease；Bacillus licheniformis, the type bacterial strain has bioflocculation
Agent, the High Cellulase Production of flocculation, Cellulose precipitates is had splendid degradation effect, multiple enzyme can be secreted simultaneously,
Ammonia nitrogen in degradable breeding water body, remaining dietary protein and starch, purifying aquatic water.
Compound water body renovation agent is the novel water body renovation agent that a class contains that microbial components is compounding with compound component, from physics
Chemical-biological many-side carries out the reparation of globality, and three kinds of reparations are complemented each other, and mutually promotes and reaches the purpose of water remediation.
Wherein bioremediation technology has the advantage of environmental friendliness, ecological energy-saving, is main body recovery technique most with prospects.
Summary of the invention
The purpose of the present invention is precisely in order to overcome the deficiency of above-mentioned technology, and provides a kind of compound water remediation containing bacillus cereus
Agent, is a kind of containing bacillus cereus and the compound water body renovation agent of compound.
It is an object of the invention to complete by following technical solution.This compound water body renovation agent containing bacillus cereus, should
Renovation agent formula composition is as follows: bacillus subtilis 1～25 parts, Bacillus licheniformis 1～25 parts, solves starch spore
Bacillus 1～25 parts, Bacillus pumilus 1～25 parts, sodium bicarbonate 10～30 parts, SODIUM PERCARBONATE 10～30 parts, percarbonic acid calcium 10～30
Part, citric acid 5～20 parts, polyaluminium sulfate 5～20 parts.
As preferably, described renovation agent formula composition is as follows: bacillus subtilis 7.5 parts, Bacillus licheniformis
7.5 parts, bacillus amyloliquefaciens 7.5 parts, Bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts, SODIUM PERCARBONATE 20 parts,
Percarbonic acid calcium 10 parts, citric acid 15 parts, polyaluminium sulfate 15 parts.
As preferably, described renovation agent formula composition is as follows: bacillus subtilis 25 parts, Bacillus licheniformis
25 parts, bacillus amyloliquefaciens 25 parts, Bacillus pumilus 25 parts, sodium bicarbonate 20 parts, SODIUM PERCARBONATE 20 parts, cross carbon
Acid calcium 20 parts, citric acid 20 parts, polyaluminium sulfate 20 parts.
As preferably, described renovation agent formula composition is as follows: bacillus subtilis 10 parts, Bacillus licheniformis
10 parts, bacillus amyloliquefaciens 10 parts, Bacillus pumilus 10 parts, sodium bicarbonate 10 parts, SODIUM PERCARBONATE 15 parts, cross carbon
Acid calcium 15 parts, citric acid 10 parts, polyaluminium sulfate 20 parts.
As preferably, described renovation agent formula composition is as follows: bacillus subtilis 12.5 parts, Bacillus licheniformis
12.5 parts, bacillus amyloliquefaciens 12.5 parts, Bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts, SODIUM PERCARBONATE 10 parts,
Percarbonic acid calcium 10 parts, citric acid 5 parts, polyaluminium sulfate 20 parts.
The invention have the benefit that the compounds such as four kinds of bacillus cereus medicinal viable bacteria powder and oxygenation agent combine by a certain percentage,
Various active product and the collaborative onset of compound；Using the compound water body of the present invention, with low cost, production technology is simple,
Can effectively reduce the COD in breeding water body, nitrous values of nitrogen might and ammonia nitrogen value, polluted-water be had and substantially repairs effect.
Detailed description of the invention
Below by detailed description of the invention, the present invention is further elaborated, and embodiment will facilitate a better understanding of the present invention, but this
Invention is not limited only to following embodiment.
This compound water body renovation agent containing bacillus cereus of the present invention, this renovation agent formula composition is as follows:
Bacillus subtilis 1～25 parts, Bacillus licheniformis 1～25 parts, bacillus amyloliquefaciens 1～25 parts, Bacillus pumilus 1～25
Part, sodium bicarbonate 10～30 parts, SODIUM PERCARBONATE 10～30 parts, percarbonic acid calcium 10～30 parts, citric acid 5～20 parts, polyaluminum sulfate
Aluminum 5～20 parts, combined each component in above ratio, and after mixing, compression molding is compound water body renovation agent.
The use of compound water body renovation agent: 1～5kg compound renovation agent is put into shrimp culture pond, takes every certain time
Water determination COD, oxygen dissolving value, nitrous values of nitrogen might and ammonia nitrogen value.The method common experimental conditions of the present invention is easy for accomplishing, operation
Simply, producing the shortest, yield is high, has wide purposes.
Wherein said bacterial strain is the most in the preservation of Chinese Typical Representative culture culture presevation administrative center, including bacillus subtilis, preservation
Number HZW1CCTCC M 2014479；Bacillus licheniformis, preserving number HZW3CCTCC M 2014480；Solve starch spore bar
Bacterium, preserving number HZW4CCTCC M 2014481, Bacillus pumilus, preserving number HZW5CCTCC M 201482.
Principle: the compounds such as four kinds of bacillus cereus medicinal viable bacteria powder and oxygenation agent are combined by a certain percentage；Bacillus cereus is medicinal
In viable bacteria powder containing viable bacteria about 30,000,000,000/gram；Compound comprises oxygenation agent SODIUM PERCARBONATE, percarbonic acid calcium, sodium bicarbonate, citric acid,
Flocculant poly aluminum sulfate.Various active product and the collaborative onset of compound, after putting into sewage, oxygenation agent the most persistently produces
A large amount of miniature oxygen bubbless.Increase water body oxygen content (DO value), meet growth of aerobic microorganisms breeding necessary factor, the biggest
Mud can be stirred by the miniature oxygen bubbles of amount, makes microorganism be fully contacted sludge components and nutrient substance.Product is thrown
After entering water body, sinking to basin bottom, tablet just gets started releasing microbe so that it is be attached to Organic substance, the oils and fats that left floating
Layer and bottom.The effervescent effect agitation of product self mud at the bottom of pond, can make microorganism be distributed in each of cesspool rapidly and uniformly
Individual corner carries out biological restoration to water body.This product can substantially repair aquaculture wastewater, reduces the float in water body, BOD, COD,
Phosphate, ammonia-nitrogen content, there is wide production application prospect.
Embodiment 1:
1, microbial inoculum: described microbial inoculum formula composition is as follows: bacillus subtilis 25 parts, Bacillus licheniformis 25
Part, bacillus amyloliquefaciens 25 parts, Bacillus pumilus 25 parts, each component to be combined in above ratio, after mixing, tabletting becomes
Type.
Adjuvant: described microbial inoculum formula composition is as follows: sodium bicarbonate 20 parts, SODIUM PERCARBONATE 20 parts, percarbonic acid
Calcium 20 parts, citric acid 20 parts, polyaluminium sulfate 20 parts.Each component being combined in above ratio, after mixing, compression molding is
Compound water body renovation agent.
Prepared by compound water body renovation agent: described renovation agent formula mix with adjuvant 1:1 by microbial inoculum after compression molding.
2, three of the above formulation types is dosed to the extracted Wastewater Sample of city inland river in 1g correspondence 10L ratio respectively, every one
Water sampling of fixing time measures float (SS), five-day BOD (BOD5), COD (COD), total nitrogen (TN),
Total phosphorus (TP) and pH value.Its experimental result is as shown in the table, and compound water body renovation agent can the most quickly improve Water quality,
Comparatively speaking, single adjuvant produces impact to water body the most in the early stage, does not have zoic long-lasting reparation；And single microbial inoculum because of
Lack the repair to water body of the adjuvant early stage, show certain lag phase.Above example shows, only when adjuvant and bacterium
Agent carries out rational compounding and gets to the repair the most long-acting to water body.
Compound water body renovation agent effect
| Index | Before feeding intake | Feed intake 48 hours | Feed intake 96 hours | Feed intake 144 hours |
| SS | 208mg/L | 15.12mg/L | 10.31mg/L | 6.19mg/L |
| BOD5 | 65.36mg/L | 32.75mg/L | 23.5mg/L | 7.12mg/L |
| CODcr | 156.47mg/L | 36.38mg/L | 16.72mg/L | 12.13mg/L |
| TN | 28.11mg/L | 13.22mg/L | 5.15mg/L | 1.05mg/L |
| TP | 8.36mg/L | 3.15mg/L | 1.51mg/L | 0.31mg/L |
| pH | 7.19 | 7.32 | 7.35 | 7.27 |
Microbial inoculum effect
| Index | Before feeding intake | Feed intake 48 hours | Feed intake 96 hours | Feed intake 144 hours |
| SS | 210mg/L | 209mg/L | 180mg/L | 133mg/L |
| BOD5 | 68.71mg/L | 60.75mg/L | 30.5mg/L | 11.12mg/L |
| CODcr | 150.33mg/L | 136.62mg/L | 66.72mg/L | 15.13mg/L |
| TN | 25.81mg/L | 23.22mg/L | 6.73mg/L | 0.75mg/L |
| TP | 8.24mg/L | 7.33mg/L | 3.11mg/L | 0.56mg/L |
| pH | 7.23 | 7.42 | 7.64 | 7.58 |
Adjuvant effect
Embodiment 2:
1, prepared by compound water body renovation agent: described renovation agent formula composition is as follows: bacillus subtilis 7.5
Part, Bacillus licheniformis 7.5 parts, bacillus amyloliquefaciens 7.5 parts, Bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts,
SODIUM PERCARBONATE 20 parts, percarbonic acid calcium 10 parts, citric acid 15 parts, polyaluminium sulfate 15 parts.By each component in above ratio group
Closing, after mixing, compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: compound for 5kg renovation agent is put into 5000m3In shrimp culture pond, every necessarily
Time water sampling is tested, data such as following table.
| Index | Before feeding intake | After feeding intake |
| SS | 47.83mg/L | 13.56mg/L |
| BOD5 | 23.93mg/L | 5.32mg/L |
| CODcr | 39.51mg/L | 10.52mg/L |
| TN | 6.72mg/L | 0.55mg/L |
| TP | 2.75mg/L | 0.25mg/L |
| pH | 7.37 | 7.38 |
Embodiment 3:
1, prepared by compound water body renovation agent: described renovation agent formula composition is as follows: bacillus subtilis 12.5
Part, Bacillus licheniformis 12.5 parts, bacillus amyloliquefaciens 12.5 parts, Bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts,
SODIUM PERCARBONATE 10 parts, percarbonic acid calcium 10 parts, citric acid 5 parts, polyaluminium sulfate 20 parts.Each component is combined in above ratio,
After mixing, compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: the inventory pressed by compound renovation agent 5kg/ days puts into 500m3Give up in pig farm
Water collecting pit, throws in 10 days continuously.Test data such as following table before and after input.
Embodiment 4:
1, prepared by compound water body renovation agent: described renovation agent formula composition is as follows: bacillus subtilis 10 parts,
Bacillus licheniformis 10 parts, bacillus amyloliquefaciens 10 parts, Bacillus pumilus 10 parts, sodium bicarbonate 10 parts, percarbonic acid
15 parts of sodium, percarbonic acid calcium 15 parts, citric acid 10 parts, polyaluminium sulfate 20 parts.Each component is combined in above ratio, mixed
Even rear compression molding is compound water body renovation agent.
2, the use of compound water body renovation agent: the inventory pressed by compound renovation agent 1kg/ days puts into 1000m3Give up in city
Water collecting pit, throws in 10 days continuously.Test data such as following table before and after input.
| Index | Before feeding intake | After feeding intake |
| SS | 580mg/L | 10.82mg/L |
| BOD5 | 130mg/L | 12.53mg/L |
| CODcr | 380mg/L | 25.2mg/L |
| TN | 48.11mg/L | 2.95mg/L |
| TP | 9.35mg/L | 0.35mg/L |
| pH | 6.87 | 7.23 |
In addition to the implementation, the present invention can also have other embodiments.The skill that all employing equivalents or equivalent transformation are formed
Art scheme, all falls within the protection domain of application claims.
Claims (5)
1. the compound water body renovation agent containing bacillus cereus, it is characterised in that: this renovation agent formula forms such as
Under: bacillus subtilis 1～25 parts, Bacillus licheniformis 1～25 parts, bacillus amyloliquefaciens 1～25 parts, Bacillus pumilus
1～25 part, sodium bicarbonate 10～30 parts, SODIUM PERCARBONATE 10～30 parts, percarbonic acid calcium 10～30 parts, citric acid 5～20 parts, poly-
Close aluminum sulfate 5～20 parts.
Compound water body renovation agent containing bacillus cereus the most according to claim 1, it is characterised in that: described reparation
Agent prescription forms as follows by weight: bacillus subtilis 7.5 parts, Bacillus licheniformis 7.5 parts, bacillus amyloliquefaciens 7.5
Part, Bacillus pumilus 7.5 parts, sodium bicarbonate 20 parts, SODIUM PERCARBONATE 20 parts, percarbonic acid calcium 10 parts, citric acid 15 parts,
Polyaluminium sulfate 15 parts.
Compound water body renovation agent containing bacillus cereus the most according to claim 1, it is characterised in that: described reparation
Agent prescription forms as follows by weight: bacillus subtilis 25 parts, Bacillus licheniformis 25 parts, bacillus amyloliquefaciens 25 parts,
Bacillus pumilus 25 parts, sodium bicarbonate 20 parts, SODIUM PERCARBONATE 20 parts, percarbonic acid calcium 20 parts, citric acid 20 parts, polymerization
20 parts of aluminum sulfate.
Compound water body renovation agent containing bacillus cereus the most according to claim 1, it is characterised in that: described reparation
Agent prescription forms as follows by weight: bacillus subtilis 10 parts, Bacillus licheniformis 10 parts, bacillus amyloliquefaciens 10 parts,
Bacillus pumilus 10 parts, sodium bicarbonate 10 parts, SODIUM PERCARBONATE 15 parts, percarbonic acid calcium 15 parts, citric acid 10 parts, polymerization
20 parts of aluminum sulfate.
Compound water body renovation agent containing bacillus cereus the most according to claim 1, it is characterised in that: described reparation
Agent prescription forms as follows by weight: bacillus subtilis 12.5 parts, Bacillus licheniformis 12.5 parts, bacillus amyloliquefaciens
12.5 parts, Bacillus pumilus 12.5 parts, sodium bicarbonate 5 parts, SODIUM PERCARBONATE 10 parts, percarbonic acid calcium 10 parts, citric acid 5
Part, polyaluminium sulfate 20 parts.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105855287A (en) * | 2016-05-30 | 2016-08-17 | 湖南恒凯环保科技投资有限公司 | Method for ex-situ remediation of organic contaminated soil by virtue of sodium percarbonate-biological pile technique |
| CN106277354A (en) * | 2016-08-26 | 2017-01-04 | 夏百庆 | A kind of duplicate removal metal water quality renovation agent |
| CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
| CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
| CN106947718B (en) * | 2017-04-14 | 2021-03-05 | 江南大学 | Method for preparing rare earth tailing improving microbial inoculum by culturing plant growth promoting bacteria by taking pig farm wastewater as raw material |
| CN108148791A (en) * | 2018-03-07 | 2018-06-12 | 天津科技大学 | A kind of probiotics preparation for improving water quality in aquaculture and preparation method thereof |
| CN108517304A (en) * | 2018-03-27 | 2018-09-11 | 丽水学院 | A kind of composite microbial Tetramune, preparation method and its application in sewage disposal |
| CN108504593A (en) * | 2018-03-27 | 2018-09-07 | 丽水学院 | A kind of compound microbial preparation, preparation method and its application in sewage disposal |
| CN108546662B (en) * | 2018-05-07 | 2021-01-29 | 中国海洋大学 | Method for jointly treating aquaculture wastewater by respectively immobilized nitrifying bacteria group-bacillus |
| CN109650556A (en) * | 2019-01-23 | 2019-04-19 | 武汉丰甜生物科技有限公司 | The composite bacillus microbial inoculum and its application of ammonia nitrogen and nitrite in degradation water body |
| CN111100830B (en) * | 2020-03-09 | 2021-11-02 | 闽江学院 | Composite microecological preparation for aquaculture water purification and application thereof |
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| KR20010054019A (en) * | 1999-12-02 | 2001-07-02 | 정준영 | A method of production of the Bioflocculant by Bacillus megaterium |
| CN101376877B (en) * | 2007-08-31 | 2011-07-27 | 天津中敖生物科技有限公司 | Compound microecological preparation for purifying cultivation water, preparation formulation thereof and preparation processes of the preparation |
| CN104176834B (en) * | 2014-08-21 | 2015-07-15 | 湖南农业大学 | Freshwater compound microorganism substrate modifier |
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