CN108504593A - A kind of compound microbial preparation, preparation method and its application in sewage disposal - Google Patents
A kind of compound microbial preparation, preparation method and its application in sewage disposal Download PDFInfo
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- CN108504593A CN108504593A CN201810260194.7A CN201810260194A CN108504593A CN 108504593 A CN108504593 A CN 108504593A CN 201810260194 A CN201810260194 A CN 201810260194A CN 108504593 A CN108504593 A CN 108504593A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a kind of compound microbial preparations, it is made of bacillus pumilus, bacillus licheniformis, aspergillus oryzae and bacillus amyloliquefaciens, in parts by weight, bacillus pumilus is 40 50 parts, bacillus licheniformis is 40 50 parts, aspergillus oryzae is 1 10 parts, and bacillus amyloliquefaciens are 1 10 parts.The invention also discloses the preparation method of the compound microbial preparation and its applications in sewage disposal.The preparation method of the compound microbial preparation of the present invention is simple, which can significantly reduce the concentration of COD in sewage, and can reduce total nitrogen concentration and total phosphorus concentration in sewage, repeatedly can continuously utilize, reduce the cost of sewage disposal.
Description
Technical field
The present invention relates to a kind of compound microbial preparation, preparation method and its applications in sewage disposal.
Background technology
With increasing year by year for world population, the discharge capacity of sanitary sewage is increasing, and the random discharge of sanitary sewage
It can lead to a series of harm, such as pathogen pollution, the dirt of aerobic organic pollution, eutrophication pollution, stench, acid-alkali salt
Dye, ground water hardness raising etc..Therefore, how to handle sanitary sewage is the problem of can not be ignored at present.
Currently, the processing mode of sanitary sewage is substantially discharged by underground piping concentration at sewage treatment plant
Reason, but the construction and operation of sewage treatment plant are not only costly, and also Wastewater Treated by Activated Sludge Process sanitary sewage is inefficient.
Microorganism formulation is to achieve the purpose that degradation of pollutant in sewage, is selected, is cultivated, combined for dirt
There is water the microbial bacteria of special degradation capability to form flora, become special sewage disposal strain, this is current sewage disposal
One of state-of-the-art several ways in technology.Microbiological treatment technology is taken the course of its own in terms of sewage disposal, it is at low cost, purification it is thorough
Bottom, environment friendly and pollution-free and easy to operate, microorganism formulation is to reach purification sewage using the metabolism of microorganism itself
Purpose, the large amount of organic in sewage can utilize by the growth and breeding of microorganism.
Usual microorganism formulation can not form good growth system in time after dispensing, therefore in sewage disposal one
After the section time, treatment effect reduces, and requires supplementation with new microorganism formulation.
Invention content
The present invention for overcome the deficiencies in the prior art, provide a kind of compound microbial preparation, preparation method and its
Application in sewage disposal.The compound microbial preparation can significantly reduce the concentration of COD in sewage, and can reduce sewage
Middle total nitrogen concentration and total phosphorus concentration repeatedly can be utilized continuously, reduce the cost of sewage disposal.
To achieve the goals above, the present invention uses following technical scheme：
A kind of compound microbial preparation, which is characterized in that be by bacillus pumilus, bacillus licheniformis, aspergillus oryzae
Bacterium and bacillus amyloliquefaciens composition, in parts by weight, bacillus pumilus is 40-50 parts, bacillus licheniformis 40-
50 parts, aspergillus oryzae is 1-10 parts, and bacillus amyloliquefaciens are 1-10 parts；
The bacillus pumilus is selected from the strain that deposit number is CGMCC NO.1271, which is named as：
Bacillus pumilus, the deposit date is 1 day January nineteen fifty-nine, depositary institution was：Institute of Microorganism, Academia Sinica is protected
Hiding address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus licheniformis is selected from the strain that deposit number is CGMCC NO.1430, which is named as：
Bacillus licheniformis, the deposit date is on December 7th, 1970, depositary institution was：Chinese Academy of Sciences microorganism is ground
Study carefully institute, preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The aspergillus oryzae is selected from the strain that deposit number is CGMCC NO.3407, which is named as Aspergillus
Oryzae, the deposit date is 1 day January nineteen fifty-two, depositary institution was：Institute of Microorganism, Academia Sinica, preservation address are：In
The Beijing state Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus amyloliquefaciens are selected from the strain that deposit number is CGMCC NO.11099, which is named as
Bacillus amyloliquefaciens, the deposit date is on October 1st, 1979, depositary institution was：The micro- life of the Chinese Academy of Sciences
Object research institute, preservation address are：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101.
The invention also discloses the preparation methods of the compound microbial preparation, include the following steps：
(1) bacillus pumilus, bacillus licheniformis and the bacillus amyloliquefaciens for preserving inclined-plane, use oese respectively
1 ring lawn is scraped, fluid nutrient medium a is seeded to and is cultivated, cultivation temperature is 37 DEG C, is cultivated 1-2 days；
(2) aspergillus oryzae for preserving inclined-plane scrapes 1 ring lawn with oese, is seeded to fluid nutrient medium b and is trained
It supports, cultivation temperature is 28 DEG C, is cultivated 1-2 days；
(3) each strain cultured solution for obtaining step (1) and (2) respectively at 6000~8000r/min centrifugation 10~
20min is filtered, is collected respectively then with sterile water washing；
(4) thalline for collecting step (3) freezes 10-12h in -20 DEG C, and 12- is then freeze-dried at -50~-60 DEG C
30h；
(5) thalline that step (4) is dried to obtain is weighed, in parts by weight, bacillus pumilus is 40-50 parts, ground
Clothing bacillus is 40-50 parts, and aspergillus oryzae is 1-10 parts, and bacillus amyloliquefaciens are 1-10 parts, and mixing is made compound micro-
Biological agent；
Fluid nutrient medium a is consisted of the following compositions described in step (1)：3 parts of beef extract, 10 parts of peptone, sodium chloride 5
Part, 1000 parts of water, above is parts by weight；It is 7.4~7.6 to adjust pH.
Fluid nutrient medium b described in step (2) is consisted of the following compositions：1 part of potassium dihydrogen phosphate, 0.5 part of magnesium sulfate, egg
White 5 parts of peptone, 10 parts of glucose, 1000 parts of water, 3.5-4 parts of 1% rose-bengal aqueous solution, above is parts by weight；Adjusting pH is
7.4~7.6.
The invention also discloses application of the compound microbial preparation in sewage disposal.Concrete application method
For：By compound microbial preparation produced by the present invention, the bacteria suspension of 2g compound microbials preparation/L is configured to sterile water,
The bacteria suspension is launched into sewage, the volume that the bacteria suspension is added is the 1% of sewage volume, and mixing is handled 7 days, same daily
One time was measured the concentration of total nitrogen in sewage, total phosphorus and COD.
The preparation method of the compound microbial preparation of the present invention is simple, which can significantly reduce
The concentration of COD in sewage, and total nitrogen concentration and total phosphorus concentration in sewage can be reduced, it repeatedly can continuously utilize, reduce dirty
The cost of water process.
Specific implementation mode
In order to make those skilled in the art be better understood from the present invention program, below in conjunction with specific embodiment to this hair
Bright technical solution carries out clear, complete description.Obviously, described embodiment is only that the part of the present invention is implemented
Example, instead of all the embodiments.
Microorganism fungus kind used in embodiment 1-4 and comparative example 1-4, wherein the bacillus thuringiensis is selected from preservation
Number is the strain of CGMCC NO.16881, which is named as：Bacillus thuringiensis, the deposit date is 2007
On June 10, in, depositary institution are：Institute of Microorganism, Academia Sinica, preservation address are：The Chinese Beijing Chaoyang District, Beijing Cities
The institute 3 of North Star West Road 1, postcode：100101；
The bacillus licheniformis is selected from the strain that deposit number is CGMCC NO.1430, which is named as：
Bacillus licheniformis, the deposit date is on December 7th, 1970, depositary institution was：Chinese Academy of Sciences microorganism is ground
Study carefully institute, preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The silver pink saccharomycete is selected from the strain that deposit number is CGMCC NO.22363, which is named as
Rhodotorula oryzae, the deposit date is on July 1st, 2002, depositary institution was：Institute of Microorganism, Academia Sinica,
Preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus amyloliquefaciens are selected from the strain that deposit number is CGMCC NO.11099, which is named as
Bacillus amyloliquefaciens, the deposit date is on October 1st, 1979, depositary institution was：The micro- life of the Chinese Academy of Sciences
Object research institute, preservation address are：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101.
Embodiment 1
The preparation of thalline, includes the following steps：
(1) bacillus thuringiensis, bacillus licheniformis and the bacillus amyloliquefaciens for preserving inclined-plane, respectively with inoculation
Ring scrapes 1 ring lawn, is seeded to fluid nutrient medium a and is cultivated, and cultivation temperature is 37 DEG C, is cultivated 1-2 days；
(2) the silver pink saccharomycete for preserving inclined-plane scrapes 1 ring lawn with oese, is seeded to fluid nutrient medium b and is trained
It supports, cultivation temperature is 28 DEG C, is cultivated 1-2 days；
(3) each strain cultured solution that step (1) and (2) obtain at 8000r/min is centrifuged into 15min respectively, then used
Sterile water washing, filtering, is collected respectively；
(4) thalline for collecting step (3) freezes 10h in -20 DEG C, and then at -55 DEG C, freeze-drying for 24 hours, is collected respectively
Thalline after drying.
Fluid nutrient medium a is consisted of the following compositions described in step (1)：Beef extract 3g, peptone 10.0g, sodium chloride
5.0g, water 1000mL, it is 7.4~7.6 to adjust pH with 1mol/L NaOH or 1mol/L HCl.
Fluid nutrient medium b described in step (2) is consisted of the following compositions：Potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.5g, egg
White peptone 5.0g, glucose 10.0g, water 1000mL, 1% rose-bengal aqueous solution 3.5mL；With 1mol/L NaOH or 1mol/L
It is 7.4~7.6 that HCl, which adjusts pH,.
Embodiment 2~4 and comparative example 1~4
According to the parts by weight of each thalline shown in table 1, thalline made from embodiment 1 is weighed respectively, then, is mixed, system
Obtain compound microbial preparation.
The parts by weight of each thalline in 1 embodiment 2~4 of table and comparative example 1~4
Embodiment 5~8 and the microorganism fungus kind used in comparative example 5~8, wherein the bacillus subtilis is selected from preservation
Number is the strain of CGMCC NO.1399, which is named as：Bacillus subtilis, the deposit date is January nineteen sixty-eight 1
Day, depositary institution is：Institute of Microorganism, Academia Sinica, preservation address are：The Chinese Beijing Chaoyang District, Beijing Cities North Star west
The institute 3 of road 1, postcode：100101；
The bacillus thuringiensis is selected from the strain that deposit number is CGMCC NO.16881, which is named as：
Bacillus thuringiensis, the deposit date is on June 10th, 2007, depositary institution was：Chinese Academy of Sciences microorganism is ground
Study carefully institute, preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The black-koji mould is selected from the strain that deposit number is CGMCC NO.36478, which is named as
Aspergillus niger, the deposit date is on December 25th, 2001, depositary institution was：Institute of Microorganism, Academia Sinica,
Preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus amyloliquefaciens are selected from the strain that deposit number is CGMCC NO.11099, which is named as
Bacillus amyloliquefaciens, the deposit date is on January 10th, 1979, depositary institution was：The micro- life of the Chinese Academy of Sciences
Object research institute, preservation address are：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101.
Embodiment 5
The preparation of thalline, includes the following steps：
(1) bacillus subtilis, bacillus thuringiensis and the bacillus amyloliquefaciens for preserving inclined-plane, respectively with inoculation
Ring scrapes 1 ring lawn, is seeded to fluid nutrient medium a and is cultivated, and cultivation temperature is 37 DEG C, is cultivated 1-2 days；
(2) black-koji mould for preserving inclined-plane scrapes 1 ring lawn with oese, is seeded to fluid nutrient medium b and is trained
It supports, cultivation temperature is 28 DEG C, is cultivated 1-2 days；
(3) each strain cultured solution that step (1) and (2) obtain at 8000r/min is centrifuged into 15min respectively, then used
Sterile water washing, filtering, is collected respectively；
(4) thalline for collecting step (3) freezes 10h in -20 DEG C, and then at -55 DEG C, freeze-drying for 24 hours, is collected respectively
Thalline after drying；
Fluid nutrient medium a is consisted of the following compositions described in step (1)：Beef extract 3g, peptone 10.0g, sodium chloride
5.0g, water 1000mL, it is 7.4~7.6 to adjust pH with 1mol/L NaOH or 1mol/L HCl.
Fluid nutrient medium b described in step (2) is consisted of the following compositions：Potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.5g, egg
White peptone 5.0g, glucose 10.0g, water 1000mL, 1% rose-bengal aqueous solution 3.5mL；With 1mol/L NaOH or 1mol/L
It is 7.4~7.6 that HCl, which adjusts pH,.
Embodiment 6~8 and comparative example 5~8
According to the parts by weight of each thalline shown in the following table 2, thalline made from embodiment 5 is weighed respectively, then, mixing,
Compound microbial preparation is made.
The parts by weight of each thalline in table 2 embodiment 6-8 and comparative example 5-8
Microorganism fungus kind used in embodiment 9-12 and comparative example 9-12, wherein the bacillus pumilus is selected from preservation
Number is the strain of CGMCC NO.1271, which is named as：Bacillus pumilus, the deposit date is January nineteen fifty-nine 1
Day, depositary institution is：Institute of Microorganism, Academia Sinica, preservation address are：The Chinese Beijing Chaoyang District, Beijing Cities North Star west
The institute 3 of road 1, postcode：100101；
The bacillus licheniformis is selected from the strain that deposit number is CGMCC NO.1430, which is named as：
Bacillus licheniformis, the deposit date is on December 7th, 1970, depositary institution was：Chinese Academy of Sciences microorganism is ground
Study carefully institute, preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The aspergillus oryzae is selected from the strain that deposit number is CGMCC NO.3407, which is named as Aspergillus
Oryzae, the deposit date is 1 day January nineteen fifty-two, depositary institution was：Institute of Microorganism, Academia Sinica, preservation address are：In
The Beijing state Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus amyloliquefaciens are selected from the strain that deposit number is CGMCC NO.11099, which is named as
Bacillus amyloliquefaciens, the deposit date is on October 1st, 1979, depositary institution was：The micro- life of the Chinese Academy of Sciences
Object research institute, preservation address are：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101.
Embodiment 9
The preparation of thalline, includes the following steps：
(1) bacillus pumilus, bacillus licheniformis and the bacillus amyloliquefaciens for preserving inclined-plane, use oese respectively
1 ring lawn is scraped, fluid nutrient medium a is seeded to and is cultivated, cultivation temperature is 37 DEG C, is cultivated 1-2 days；
(2) aspergillus oryzae for preserving inclined-plane scrapes 1 ring lawn with oese, is seeded to fluid nutrient medium b and is trained
It supports, cultivation temperature is 28 DEG C, is cultivated 1-2 days；
(3) each strain cultured solution that step (1) and (2) obtain at 8000r/min is centrifuged into 15min respectively, then used
Sterile water washing, filtering, is collected respectively；
(4) thalline for collecting step (3) freezes 10h in -20 DEG C, and then at -55 DEG C, freeze-drying for 24 hours, is collected respectively
Thalline after drying；
Fluid nutrient medium a is consisted of the following compositions described in step (1)：Beef extract 3g, peptone 10.0g, sodium chloride
5.0g, water 1000mL, it is 7.4~7.6 to adjust pH with 1mol/L NaOH or 1mol/L HCl.
Fluid nutrient medium b described in step (2) is consisted of the following compositions：Potassium dihydrogen phosphate 1.0g, magnesium sulfate 0.5g, egg
White peptone 5.0g, glucose 10.0g, water 1000mL, 1% rose-bengal aqueous solution 3.5mL；With 1mol/L NaOH or 1mol/L
It is 7.4~7.6 that HCl, which adjusts pH,.
Embodiment 10-12 and comparative example 9-12
According to the parts by weight of each thalline shown in the following table 3, thalline made from embodiment 9 is weighed respectively, then, mixing,
Compound microbial preparation is made respectively.
The parts by weight of each thalline in table 3 embodiment 10-12 and comparative example 9-12
Embodiment 13
By compound microbial preparation made from above-described embodiment 2~4,6~8,10~12 and comparative example 1~12, respectively
It is configured to the bacteria suspension of 2g compound microbials preparation/L with sterile water, which is launched into sewage respectively, being added should
The volume of bacteria suspension is the 1% of sewage volume, and mixing is handled 7 days, and the daily same time is measured total nitrogen in sewage, total phosphorus
With the concentration of COD.It takes three parts of isometric sewage to be measured every time, takes the average value of concentration three times as ultimate density.
Before compound microbial preparation is added, the initial concentration of total nitrogen, total phosphorus and COD is respectively in sanitary sewage：
20.01mg/L, 4.51mg/L, 171.01mg/L.
As a result：
Table 1-1：Embodiment 2~4 handles sanitary sewage with compound microbial preparation prepared by comparative example 1~4, wherein always
The concentration variation (mg/L) of nitrogen
| Number of days | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Embodiment 4 | Embodiment 3 | Embodiment 2 |
| 0 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 |
| 1 | 19.10 | 19.03 | 19.11 | 19.01 | 18.81 | 18.92 | 18.85 |
| 2 | 18.12 | 17.11 | 17.21 | 16.92 | 16.69 | 16.86 | 16.77 |
| 3 | 17.01 | 14.87 | 14.68 | 14.56 | 13.98 | 14.11 | 14.06 |
| 4 | 15.83 | 12.33 | 12.25 | 12.01 | 11.96 | 12.16 | 12.03 |
| 5 | 14.25 | 11.59 | 11.21 | 11.09 | 11.04 | 11.45 | 11.32 |
| 6 | 14.23 | 11.60 | 11.02 | 10.83 | 10.55 | 10.96 | 10.81 |
| 7 | 14.22 | 11.58 | 10.86 | 10.62 | 10.06 | 10.47 | 10.32 |
The compound microbial preparation of the preparation of embodiment 2~4 is can be seen that sewage disposal 7 days from the data in table 1-1
Afterwards, total nitrogen concentration decreases in sewage, but reduction is less, illustrates this processing of compound microbial preparation to total nitrogen in sewage
Ability is general.
Table 1-2：Embodiment 2~4 handles sanitary sewage with compound microbial preparation prepared by comparative example 1~4, wherein always
The concentration variation (mg/L) of phosphorus
As can be seen that this compound microbial preparation, can remove the total phosphorus in sanitary sewage from table 1-2,
Removal rate is apparently higher than the treatment effeciency to total nitrogen up to 94% or more, to the treatment effeciency of total phosphorus.Compound micro- life of the present invention
Four kinds of thalline synergistic effects, higher to the processing capacity of total phosphorus in sewage in object preparation.
Embodiment 2 is compared with compound microbial preparation prepared by comparative example 2, and difference lies in add the two in embodiment 2
Bacillus amyloliquefaciens are entered, the effect of treated sewage is higher than comparative example 2.Increase with time, in the sewage that comparative example 2 is handled
Total phosphorus concentration almost no longer changes, because the active constituent of the compound microbial preparation prepared in comparative example 2 is constantly consumed,
Cause treated sewage effect to continuously decrease, finally loses further processing capacity.However, after 5 days, the processing of embodiment 2
Sewage in total phosphorus concentration still continuing to reduce, this illustrates that bacillus amyloliquefaciens have growth-promoting effect to other strains,
An adaptive growth system can be formed, so that the processing capacity of compound microbial preparation is continued；So that
Compound microbial preparation can constantly in treated sewage total phosphorus, without supplementing new compound microbial preparation.
After 5 days, compound microbial preparation treated sewage prepared by comparative example 3 and comparative example 4, wherein total phosphorus concentration
Also continuing to reduce, further illustrate bacillus amyloliquefaciens has growth-promoting effect to other strains.
To sum up, bacillus thuringiensis, bacillus licheniformis, silver pink saccharomycete conciliate starch in compound microbial preparation
Bacillus acts synergistically, stronger to the processing capacity of total phosphorus in sewage.
Table 1-3：Embodiment 2~4 handles sanitary sewage with compound microbial preparation prepared by comparative example 1~4, wherein
The concentration variation (mg/L) of COD
| Number of days | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Embodiment 4 | Embodiment 3 | Embodiment 2 |
| 0 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 |
| 1 | 161.63 | 152.37 | 151.10 | 150.41 | 149.91 | 150.05 | 150.01 |
| 2 | 150.91 | 133.78 | 126.12 | 124.82 | 123.85 | 123.91 | 123.84 |
| 3 | 141.05 | 118.10 | 113.07 | 111.23 | 110.63 | 111.12 | 111.09 |
| 4 | 119.74 | 109.73 | 104.62 | 101.98 | 101.81 | 102.10 | 102.25 |
| 5 | 110.56 | 100.31 | 96.85 | 93.82 | 92.94 | 93.43 | 93.31 |
| 6 | 110.55 | 100.28 | 92.86 | 89.83 | 88.62 | 89.05 | 89.01 |
| 7 | 110.53 | 100.27 | 88.95 | 86.15 | 84.01 | 85.13 | 84.91 |
As can be seen that the compound microbial preparation can reduce COD concentration in sanitary sewage from table 1-3, but imitate
Fruit is general, and in comparison, which is apparently higher than the effect of the reduction to COD concentration to the treatment effeciency of total phosphorus
Rate.
Table 2-1：Compound microbial preparation prepared by embodiment 6-8 and comparative example 5-8 handles sanitary sewage, wherein total nitrogen
Concentration variation (mg/L)
| Number of days | Comparative example 5 | Comparative example 6 | Comparative example 7 | Comparative example 8 | Embodiment 8 | Embodiment 7 | Embodiment 6 |
| 0 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 | 20.01 |
| 1 | 19.21 | 19.11 | 19.03 | 19.14 | 17.33 | 17.80 | 17.59 |
| 2 | 18.23 | 17.22 | 16.89 | 17.23 | 13.75 | 14.09 | 13.86 |
| 3 | 17.13 | 14.97 | 14.55 | 14.69 | 8.76 | 9.75 | 9.33 |
| 4 | 15.92 | 12.41 | 12.31 | 12.37 | 4.25 | 4.46 | 4.58 |
| 5 | 14.36 | 11.61 | 11.17 | 11.30 | 2.17 | 2.57 | 2.41 |
| 6 | 14.35 | 11.60 | 10.91 | 11.12 | 1.23 | 1.76 | 1.54 |
| 7 | 14.34 | 11.59 | 10.70 | 10.88 | 0.64 | 1.15 | 0.96 |
The compound microbial preparation of the preparation of embodiment 6~8 is can be seen that sewage disposal 7 days from the data in table 2-1
Afterwards, total nitrogen concentration has significantly reduced in sewage, and removal rate is stronger to the processing capacity of total nitrogen in sewage up to 95% or more.
Embodiment 6 is compared with compound microbial preparation prepared by comparative example 6, and difference lies in add the two in embodiment 6
Bacillus amyloliquefaciens are entered.Increase with time, total nitrogen concentration almost no longer changes in the sewage that comparative example 6 is handled, because right
The active constituent of the compound microbial preparation prepared in ratio 6 is constantly consumed, and causes treated sewage effect to continuously decrease, most
After lose further processing capacity.However, after 5 days, total nitrogen concentration still is continuing to drop in the sewage of the processing of embodiment 6
Low, this illustrates that bacillus amyloliquefaciens have growth-promoting effect to other strains, can form an adaptive growth body
System, so that the processing capacity of compound microbial preparation is continued；Enable compound microbial preparation constantly
Treated sewage, without supplementing new compound microbial preparation.
After 5 days, compound microbial preparation treated sewage prepared by comparative example 7 and comparative example 8, wherein total nitrogen concentration
Also continuing to reduce, further illustrate bacillus amyloliquefaciens has growth-promoting effect to other strains.
To sum up, bacillus subtilis, bacillus thuringiensis, black-koji mould conciliate starch bud in compound microbial preparation
Spore bacillus acts synergistically, and can effectively reduce the total nitrogen concentration in sewage.
Table 2-2 embodiments 6~8 handle sanitary sewage with compound microbial preparation prepared by comparative example 5~8, wherein always
The concentration variation (mg/L) of phosphorus
The compound microbial preparation of the preparation of embodiment 6~8 is can be seen that sewage disposal 7 days from the data in table 2-2
Afterwards, total phosphorus concentration decreases in sewage, but reduction is less, this illustrates that this compound microbial preparation has total phosphorus in sewage
Certain processing capacity, but treatment effect is general.
Compound microbial preparation prepared by table 2-3 embodiments 6-8 and comparative example 5-8 handles sanitary sewage, wherein COD's
Concentration changes (mg/L)
| Number of days | Comparative example 5 | Comparative example 6 | Comparative example 7 | Comparative example 8 | Embodiment 8 | Embodiment 7 | Embodiment 6 |
| 0 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 | 171.01 |
| 1 | 161.52 | 151.46 | 151.21 | 150.52 | 149.62 | 150.71 | 150.17 |
| 2 | 150.83 | 132.81 | 126.23 | 123.90 | 122.93 | 123.12 | 123.08 |
| 3 | 141.03 | 117.14 | 113.12 | 110.32 | 109.35 | 110.50 | 110.31 |
| 4 | 119.62 | 108.85 | 104.71 | 100.91 | 100.11 | 101.24 | 101.17 |
| 5 | 110.47 | 99.42 | 96.92 | 92.40 | 91.55 | 92.71 | 92.16 |
| 6 | 110.46 | 99.41 | 93.90 | 88.61 | 86.46 | 87.83 | 87.28 |
| 7 | 110.45 | 99.42 | 91.87 | 86.10 | 83.13 | 84.94 | 84.72 |
The compound microbial preparation of the preparation of embodiment 6~8 is can be seen that sewage disposal 7 days from the data in table 2-3
Afterwards, total phosphorus concentration decreases in sewage, but reduces less, drop of this compound microbial preparation to COD concentration in sewage
Inefficient fruit is general.
Table 3-1 embodiments 10~12 handle sanitary sewage with compound microbial preparation prepared by comparative example 9~12, wherein
The concentration variation (mg/L) of total nitrogen
The compound microbial preparation of the preparation of embodiment 10~12 is can be seen that sewage from the experimental data in table 3-1
After processing 7 days, total nitrogen concentration is decreased in sewage, but reduction is less, this compound microbial preparation is to total nitrogen in sewage
Processing capacity it is general.
Table 3-2 embodiments 10~12 handle sanitary sewage with compound microbial preparation prepared by comparative example 9~12, wherein
The concentration variation (mg/L) of total phosphorus
The compound microbial preparation of the preparation of embodiment 10~12 is can be seen that sewage from the experimental data in table 3-2
After processing 7 days, total phosphorus concentration is decreased in sewage, but reduction is less, this compound microbial preparation is to total nitrogen in sewage
Treatment effect it is general.
Table 3-3 embodiments 10~12 handle sanitary sewage with compound microbial preparation prepared by comparative example 9~12, wherein
The concentration variation (mg/L) of COD
The compound microbial preparation of the preparation of embodiment 10~12 is can be seen that sewage disposal 7 from the data in table 3-3
After it, COD concentration significantly reduces, and for removal rate up to 98% or more, treatment effeciency is apparently higher than embodiment 6~8 and 2~4.
Embodiment 10 is compared with compound microbial preparation prepared by comparative example 10, and difference lies in embodiments 10 for the two
In add bacillus amyloliquefaciens.Increase with time, COD concentration almost no longer changes in the sewage that comparative example 10 is handled, because
The active constituent of compound microbial preparation to be prepared in comparative example 10 is constantly consumed, and treated sewage effect is caused gradually to drop
It is low, finally lose further processing capacity.However, after 5 days, in the sewage of the processing of embodiment 10 COD concentration still after
Continuous to reduce, this illustrates that bacillus amyloliquefaciens have growth-promoting effect to other strains, can form an adaptive life
Long system, so that the processing capacity of compound microbial preparation is continued；Compound microbial preparation is held
COD concentration in sewage is reduced continuously.
After 5 days, compound microbial preparation treated sewage prepared by comparative example 11 and comparative example 12, wherein COD is dense
Degree also is continuing to reduce, and further illustrate bacillus amyloliquefaciens has growth-promoting effect to other strains.
To sum up, bacillus pumilus, bacillus licheniformis, aspergillus oryzae conciliate starch gemma in compound microbial preparation
Bacillus acts synergistically, and can significantly reduce the concentration of COD in sewage.
Obviously, described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment should all belong to the scope of protection of the invention.
Claims (8)
1. a kind of compound microbial preparation, which is characterized in that be by bacillus pumilus, bacillus licheniformis, aspergillus oryzae
It is formed with bacillus amyloliquefaciens, in parts by weight, bacillus pumilus is 40-50 parts, bacillus licheniformis 40-50
Part, aspergillus oryzae is 1-10 parts, and bacillus amyloliquefaciens are 1-10 parts；
The bacillus pumilus is selected from the strain that deposit number is CGMCC NO.1271, which is named as：Bacillus
Pumilus, the deposit date is 1 day January nineteen fifty-nine, depositary institution was：Institute of Microorganism, Academia Sinica, preservation address are：
The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus licheniformis is selected from the strain that deposit number is CGMCC NO.1430, which is named as：Bacillus
Licheniformis, the deposit date is on December 7th, 1970, depositary institution was：Institute of Microorganism, Academia Sinica, preservation
Address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The aspergillus oryzae is selected from the strain that deposit number is CGMCC NO.3407, which is named as Aspergillus
Oryzae, the deposit date is 1 day January nineteen fifty-two, depositary institution was：Institute of Microorganism, Academia Sinica, preservation address are：In
The Beijing state Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101；
The bacillus amyloliquefaciens are selected from the strain that deposit number is CGMCC NO.11099, which is named as Bacillus
Amyloliquefaciens, the deposit date is on October 1st, 1979, depositary institution was：Institute of Microorganism, Academia Sinica,
Preservation address is：The Chinese Beijing Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3s, postcode：100101.
2. a kind of preparation method of compound microbial preparation, which is characterized in that this method is used to prepare described in claim 1
Compound microbial preparation, includes the following steps：
(1) bacillus pumilus, bacillus licheniformis and the bacillus amyloliquefaciens for preserving inclined-plane, are scraped with oese respectively
1 ring lawn is seeded to fluid nutrient medium a and is cultivated, and cultivation temperature is 37 DEG C, is cultivated 1-2 days；
(2) aspergillus oryzae for preserving inclined-plane scrapes 1 ring lawn with oese, is seeded to fluid nutrient medium b and is cultivated, trains
It is 28 DEG C to support temperature, is cultivated 1-2 days；
(3) each strain cultured solution for obtaining step (1) and (2) centrifuges 10~20min at 6000~8000r/min respectively,
Then it with sterile water washing, filters, collects respectively；
(4) thalline for collecting step (3) freezes 10-12h in -20 DEG C, and 12-30h is then freeze-dried at -50~-60 DEG C；
(5) thalline that step (4) is dried to obtain is weighed, in parts by weight, bacillus pumilus is 40-50 parts, lichens bud
Spore bacillus is 40-50 parts, and aspergillus oryzae is 1-10 parts, and bacillus amyloliquefaciens are 1-10 parts, and compound microbial is made in mixing
Preparation.
3. the preparation method of compound microbial preparation according to claim 2, which is characterized in that described in step (1)
Fluid nutrient medium a is consisted of the following compositions：3 parts of beef extract, 10 parts of peptone, 5 parts of sodium chloride, 1000 parts of water, above is weight
Number；It is 7.4~7.6 to adjust pH.
4. the preparation method of compound microbial preparation according to claim 2, which is characterized in that described in step (2)
Fluid nutrient medium b consist of the following compositions：1 part of potassium dihydrogen phosphate, 0.5 part of magnesium sulfate, 5 parts of peptone, 10 parts of glucose, water
1000 parts, 3.5-4 parts of 1% rose-bengal aqueous solution, above is parts by weight；It is 7.4~7.6 to adjust pH.
5. application of the compound microbial preparation described in claim 1 in sewage disposal.
6. the compound microbial preparation obtained by the preparation method according to any one of claim 2-4 is in sewage disposal
In application.
7. application according to claim 5, which is characterized in that application process is：It will be compound described in claim 1
Microorganism formulation is configured to the bacteria suspension of 2g compound microbials preparation/L with sterile water, which is launched into sewage,
The volume that the bacteria suspension is added is the 1% of sewage volume, and mixing is handled 7 days, and the daily same time is measured in sewage always
The concentration of nitrogen, total phosphorus and COD.
8. application according to claim 6, which is characterized in that application process is：By the system of any one of claim 2-4
Compound microbial preparation obtained by Preparation Method is configured to the bacteria suspension of 2g compound microbials preparation/L with sterile water, will
The bacteria suspension is launched into sewage, and the volume that the bacteria suspension is added is the 1% of sewage volume, and mixing is handled 7 days, same daily
Time is measured the concentration of total nitrogen in sewage, total phosphorus and COD.
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