CN106497813B - Serratia marcescens and microbial inoculum and their applications in degrading polycyclic aromatic hydrocarbons - Google Patents
Serratia marcescens and microbial inoculum and their applications in degrading polycyclic aromatic hydrocarbons Download PDFInfo
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Abstract
The present invention relates to microorganism remediation environmental areas, specifically disclose one plant of serratia marcescens (Serratia marcescens), wherein the deposit number of the serratia marcescens is CGMCC No.10847, and the microbial inoculum containing the bacterial strain.The application and a kind of method of degrading polycyclic aromatic hydrocarbons that the invention also discloses bacterial strains as described above and/or microbial inoculum in degrading polycyclic aromatic hydrocarbons.Serratia marcescens (Serratia marcescens) of the present invention is under conditions of being provided only with carbon source, nitrogen source and inorganic salts, it can survive and grow in the culture environment that polycyclic aromatic hydrocarbon (such as naphthalene) is sole carbon source, vitality is strong, the speed of growth is fast, biomass is big, has very high tolerance and degradation capability to polycyclic aromatic hydrocarbon.
Description
Technical field
The present invention relates to microbial environments to repair field, and in particular, to one plant of new serratia marcescens, it is viscous containing this
The microbial inoculum of matter Serratieae and a kind of their applications and method of degrading polycyclic aromatic hydrocarbons in degrading polycyclic aromatic hydrocarbons.
Background technique
Polycyclic aromatic hydrocarbon (Polycyclic aromatic hydrocarbons, PAHs) is widely distributed in the environment, is one
Class chemical structure is complicated, the polluter with mutagenicity and carcinogenicity.It can be enriched with by the transmitting of biological chain, right
Environment and the mankind cause great harm.
Naphthalene (naphthalene) is the low molecule polycyclic arene compound being made of two phenyl ring, natural environment such as
It is widely distributed in atmosphere, soil, water and its deposit.The polycyclic aromatic hydrocarbons such as naphthalene have " three cause " effect, and (carcinogenic, teratogenesis and cause are prominent
Become), the health of different kind organism is not only directly endangered, but also since it is difficult to degrade, often passes through organism accumulation and biomagnification
Human health is endangered, while also seriously destroying ecological environment.Therefore, the effective ways for eliminating this pollutant are found as complete
One of great environmental problem of World Focusing.
Summary of the invention
The object of the present invention is to provide one kind conveniently and effectively and environmentally friendly to drop to the polycyclic aromatic hydrocarbon in environment
The method of solution.
To achieve the goals above, on the one hand, the present invention provides one plant of serratia marcescens (Serratia
Marcescens), wherein the deposit number of the serratia marcescens is CGMCC No.10847.
Second aspect, the present invention provides a kind of microbial inoculums, wherein the microbial inoculum contains serratia marcescens as described above
(Serratia marcescens)。
The third aspect, the present invention provides serratia marcescens as described above and/or microbial inoculum as described above to degrade
Application in polycyclic aromatic hydrocarbon.
Preferably, the polycyclic aromatic hydrocarbon is naphthalene.
Fourth aspect, the present invention provides a kind of methods of degrading polycyclic aromatic hydrocarbons, this method comprises: will glue as described above
Matter Serratieae (Serratia marcescens), and/or microbial inoculum as described above are contacted with the environment containing polycyclic aromatic hydrocarbon,
To degrade to the polycyclic aromatic hydrocarbon in environment.
Preferably, the polycyclic aromatic hydrocarbon is naphthalene.
Preferably, this method further includes, during to the degrading polycyclic aromatic hydrocarbons, to described containing polycyclic aromatic hydrocarbon
Inorganic salts are added in environment.
Preferably, when serratia marcescens as described above is added into the environment containing polycyclic aromatic hydrocarbon
When (Serratia marcescens), the serratia marcescens of addition is by the solid phase after Liquid Culture and separation of solid and liquid
And/or the bacterium colony after solid culture provides.
Serratia marcescens (Serratia marcescens) of the present invention is being provided only with carbon source, nitrogen source and nothing
It under conditions of machine salt, i.e., survives and grows in the culture environment that polycyclic aromatic hydrocarbon (such as naphthalene) is sole carbon source, vitality is strong, raw
Long speed is fast, and biomass is big, has very high tolerance and degradation capability to polycyclic aromatic hydrocarbon.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biological deposits
Bacterial strain of the invention was named as serratia marcescens (Serratia marcescens), and May 22 in 2015
It is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address: the Chaoyang District, Beijing City North Star west day
The institute 3 of road 1, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution is abbreviated as CGMCC) protects
Hiding number is CGMCC No.10847, referred to as bacterial strain QD-G.
Detailed description of the invention
The drawings are intended to provide a further understanding of the invention, and constitutes part of specification, with following tool
Body embodiment is used to explain the present invention together, but is not construed as limiting the invention.In the accompanying drawings:
Fig. 1 is that naphthalene removal rate answers the variation of time in pedotheque under different recovery scenarios.
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
In a first aspect, the present invention provides one plant of serratia marcescens (Serratia marcescens), wherein described
The deposit number of serratia marcescens is CGMCC No.10847.
Serratia marcescens (Serratia marcescens) of the invention is isolated from the soil of Hebei Handan contaminated by diesel oil
Sample.
According to the present invention, the method for separating the serratia marcescens can be the side of the conventional screening novel bacterial in this field
Method, such as the separation method may include: to be added to the pedotheque 10g from Hebei Handan contaminated by diesel oil of acquisition
In the MSM culture medium of 100mL, 4-6h is cultivated under the conditions of 25-35 DEG C and 130-170rpm.MSM suspension containing soil is existed
It is centrifuged 5min under about 3000rpm, 10mL supernatant is added in selective MSM fluid nutrient medium of the 100mL containing naphthalene and is commissioned to train through 4
After supporting (naphthalene concentration is incremented by with the gradient of 50mg/L, 100mg/L, 150mg/L, 200mg/L), it is coated with after taking appropriate culture solution to dilute
On the selective MSM solid plate containing naphthalene, when having obvious bacterium colony to occur, therefrom selection size, the bacterium colony to come in every shape exist
Further scribing line purifies and separates are carried out on LB solid medium.By strain inoculated respectively after purification in the selective MSM liquid containing naphthalene
Its growing state is observed in body culture medium, and the good bacterial strain of growing state is prepared into bacterial suspension inoculation to containing naphthalene 0.5%
In 200g autogamy contaminated soil, pedotheque is placed in 25 DEG C of artificial climate incubators, stir daily soil and be added go from
Sub- water measures naphthalene content in soil after making soil moisture content (20 ± 2) %, 10d, chooses the highest bacterium of degradation rate as efficient
Naphthalene degradation bacteria, to obtain serratia marcescens of the invention (Serratia marcescens), and May 22 in 2015
Day is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number CGMCC
The nucleotide sequence of No.10847,16s rDNA are as shown in SEQ ID No:1.
According to the present invention, the composition of the MSM fluid nutrient medium can be with are as follows: poidometer, the KH containing 0.8-1.2%2PO4,
The K of 0.8-1.2%2HPO4, the NH of 1-1.5%4NO3, the MgSO of 0.03-0.08%4, the CaCl of 0.001-0.003%2, 0.01-
0.03% FeSO4·7H2O, pH value can be 6.0-7.5.
Wherein, the agar of 1-2 weight % can be added in MSM solid medium in MSM fluid nutrient medium as described above
It is prepared.
Serratia marcescens provided by the invention can generate the viable bacteria body of a large amount of serratia marcescens by culture, described
The method of culture does not require particularly, as long as the serratia marcescens can be made to be proliferated, such as can be according to
107The viable bacteria body of serratia marcescens is inoculated in LB culture medium by the inoculum concentration of CFU/mL, and under aerobic condition,
After cultivating 8-72 hours at a temperature of 25-38 DEG C, culture solution is obtained.
Serratia marcescens (Serratia marcescens) provided by the invention is inoculated with the inoculum concentration of 2-5 volume %
In LB culture medium, under 150-240rpm, 25-35 DEG C of condition of culture, 7h can enter logarithmic phase, and 17h reaches stationary phase,
Bacteria concentration OD600Highest can about 9.
The present invention can further separate the viable bacteria body of the serratia marcescens in above-mentioned culture solution, the isolated method
It is not particularly limited, as long as thallus can be enriched with from culture solution, such as can method by being centrifuged and/or filtering
It realizes, the condition of the centrifugation and the filtering can be well known condition, and details are not described herein by the present invention.
Second aspect, the present invention provides a kind of microbial inoculums, wherein the microbial inoculum contains serratia marcescens as described above
(Serratia marcescens)。
According to the present invention, in the microbial inoculum, the concentration of the serratia marcescens is not particularly limited, can basis
Particular situation is specifically selected, and in this not go into detail.
In addition, microbial inoculum provided by the invention can be prepared as different dosage forms, and be added with according to intended purpose difference
The ingredients such as the excipient that the activity of the serratia marcescens will not be impacted accordingly.Specifically it is selected as this field skill
Well known to art personnel, in this not go into detail by the present invention.
The third aspect, the present invention also provides serratia marcescens as described above and/or microbial inoculum as described above to drop
Solve the application in polycyclic aromatic hydrocarbon.
Serratia marcescens provided by the invention is only needing there are under conditions of carbon source, nitrogen source and inorganic salts, i.e., more
Cycloaromatics is survived and is grown as in the inorganic salts culture environment of sole carbon source, and vitality is strong, and the speed of growth is fast, and biomass is big,
There are very high tolerance and degradation capability to polycyclic aromatic hydrocarbon.
, according to the invention it is preferred to, the polycyclic aromatic hydrocarbon is naphthalene.
Fourth aspect, the present invention provides a kind of methods of degrading polycyclic aromatic hydrocarbons, this method comprises: will glue as described above
Matter Serratieae (Serratia marcescens) and/or microbial inoculum as described above are contacted with the environment containing polycyclic aromatic hydrocarbon, with
It degrades to the polycyclic aromatic hydrocarbon in environment.
Preferably, the polycyclic aromatic hydrocarbon is naphthalene.
According to the present invention, the contact preferably carries out under conditions of the serratia marcescens can survive.Wherein, art
Language " serratia marcescens can survive " refers in the environment containing polycyclic aromatic hydrocarbon, at least about 20%, preferably at least it is greater than
40%, the more preferably at least thallus greater than 60% can survive.Term " condition that serratia marcescens can survive " refer to
It less include carbon source, the condition for being able to maintain that thallus vitality of nitrogen source and inorganic salts.The carbon source can be for example its degradation
Polycyclic aromatic hydrocarbon.
According to the present invention, described containing polycyclic aromatic hydrocarbons contaminated environment may include any environment containing polycyclic aromatic hydrocarbon,
It containing polycyclic aromatic hydrocarbons contaminated environment may include soil or water body containing polycyclic aromatic hydrocarbon for example, described.
According to the present invention, it is added to the form containing the serratia marcescens in polycyclic aromatic hydrocarbons contaminated environment not
There is special restriction, as long as guaranteeing that the serratia marcescens can be described containing in polycyclic aromatic hydrocarbons contaminated environment after being added
It works and effectively degrades to the polycyclic aromatic hydrocarbon, the form of the serratia marcescens of addition, for example, can be with
To cultivate to the activation thallus (bacteria suspension or bacterial sediment) of logarithmic phase, or the thalli dry powder after freeze-drying, or
The microbial inoculum obtained after compounding as above is preferably cultivated to the activation thallus of logarithmic phase.
The present invention is also not particularly limited the serratia marcescens quantity of addition, this can be according to described containing polycyclic
The survival ability in the environment of content and bacterial strain of polycyclic aromatic hydrocarbon in the environment of aromatic hydrocarbons pollution determines, for example, working as
It, can be with when polycyclic aromatic hydrocarbon content in the environment is higher or the environment is less favorable for the existence of the serratia marcescens
Improve the additional amount of the serratia marcescens；When the polycyclic aromatic hydrocarbon content in the environment is lower or the environment is to described viscous
When the survival effect of matter Serratieae is smaller, it is possible to reduce the additional amount of the serratia marcescens.
The content containing polycyclic aromatic hydrocarbons contaminated Polycyclic Aromatic Hydrocarbonat Existing in Environment is also not particularly limited in the present invention,
But the serratia marcescens is comprehensively considered containing the survival ability in polycyclic aromatic hydrocarbons contaminated and to the degradation of polycyclic aromatic hydrocarbon
Effect, relative to polycyclic aromatic hydrocarbons contaminated environment is contained described in every kilogram or every liter, the content of the polycyclic aromatic hydrocarbon is not higher than
1000mg, preferably no greater than 800mg, more preferably no higher than 500mg, even more preferably from no more than 300mg.
According to the present invention, in order to further increase serratia marcescens provided by the invention containing polycyclic aromatic hydrocarbons contaminated
Survival ability and activity in environment, to improve the degradation rate to polycyclic aromatic hydrocarbon, method of the invention further includes to described
Additional inorganic salts are provided in environment.
Wherein, the type of the additional inorganic salts can be to be well known in the art for cultivating the nothing of serratia marcescens
The type of machine salt.Preferably, the inorganic salts can be selected from KH2PO4, K2HPO4, NH4NO3, MgSO4, CaCl2And FeSO4In
It is one or more, more preferably KH2PO4, K2HPO4, NH4NO3, MgSO4, CaCl2And FeSO4·7H2O。
Wherein, the present invention is not particularly limited the amount of the inorganic salts of addition, can be according to described containing polycyclic
In the environment of aromatic hydrocarbons pollution depending on the type and content of inorganic salts.For example, with KH2PO4(0.8-1.2 weight %), K2HPO4
(0.8-1.2 weight %), NH4NO3(1-1.5 weight %), MgSO4(0.03-0.08 weight %), CaCl2(0.001-0.003 weight
Measure %), FeSO4·7H2It is more based on containing described in every kilogram for the mixed inorganic salt solution of O (0.01-0.03 weight %)
The environment of cycloaromatics pollution, the additional amount of above-mentioned inorganic salts mixed liquor can be 1-3ml/ days.
According to the present invention, when the environment containing polycyclic aromatic hydrocarbon is soil, in order to further promote offer of the present invention
Serratia marcescens to the degradation efficiency of polycyclic aromatic hydrocarbon, it is preferred that by the water content control in soil at least 15 weight %,
More preferably 18-30 weight %.
In addition, in the present invention, when Serratia as described above is added in the environment containing polycyclic aromatic hydrocarbon
When bacterium (Serratia marcescens), the serratia marcescens is preferably added to by after Liquid Culture and separation of solid and liquid
Solid phase and/or the bacterium colony after solid culture, in the preferred situation, serratia marcescens provided by the invention is to polycyclic
The degradation effect of aromatic hydrocarbons is more preferably.
According to the present invention, solid phase of the serratia marcescens after Liquid Culture and separation of solid and liquid further preferably uses physiology
Salt water is washed.
The present invention will be described in detail by way of examples below.
In following embodiment:
LB liquid medium: 0.9 weight % peptone, 0.7 weight % yeast powder, 1.2 weight % sodium chloride, pH=6.8-
7.0。
MSM minimal medium: KH2PO4(1.0 weight %), K2HPO4(1.0 weight %), NH4NO3(1.2 weight %),
MgSO4(0.05 weight %), CaCl2(0.002 weight %), FeSO4·7H2O (0.02 weight %), pH=7.
Bacterial strain serratia marcescens (Serratia marcescens) of the invention, hereinafter referred to as bacterial strain QD-G, and in
On May 22nd, 2015 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address: court, Beijing
The institute 3 of positive area's North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution is abbreviated as
CGMCC), deposit number is CGMCC No.10847, referred to as bacterial strain QD-G.
Reference strains are serratia marcescens hereinafter referred to as bacterial strain D, are isolated from in batch naphthalene contaminated soil.
Preparation example
After bacterial strain QD-G and reference strains D of the invention are inoculated with respectively in LB culture medium with the inoculum concentration of 1 volume %,
30 ± 1 DEG C, cultivate under conditions of 150rpm 17 hours it is spare.
Embodiment 1
The present embodiment is for illustrating serratia marcescens provided by the invention in LB culture solution to naphthalene tolerance and degradation
Property
By bacterial strain QD-G containing concentration to be respectively 10mg/L, 20mg/L after the access sterilizing of the inoculum concentration of 1 volume %,
In the LB liquid medium of 50mg/L, 100mg/L, 200mg/L naphthalene.It cultivates under the conditions of 170rpm, 30 ± 1 DEG C for 24 hours, as a result shows
Show, bacterial strain QD-G shows good tolerance to naphthalene, and bacterial strain can be grown in different medium.The attainable maximum of thallus
OD value and 1 is shown in Table to the degradation rate of naphthalene.
Comparative example 1-1
This comparative example is used to illustrate the bacterial strain D of reference in LB culture solution to the tolerance of naphthalene and degradability
The LB culture medium containing different naphthalene concentration is prepared according to the method for embodiment 1 and it is handled, unlike,
The bacterial strain of access is reference strains D.The attainable maximum OD value of thallus and 1 is shown in Table to the degradation rate of naphthalene.
Comparative example 1-2
This comparative example is used to illustrate the degradability (blank control) of the naphthalene in LB culture solution under natural conditions
The LB culture medium containing different naphthalene concentration is prepared according to the method for embodiment 1 and it is handled, unlike,
It is not inoculated with any strain, but same amount of LB liquid medium is added.The maximum OD value of culture solution and the degradation rate of naphthalene are shown in
Table 1.
Table 1
As can be seen from Table 1, in LB culture medium, compared with bacterial strain D, bacterial strain QD-G of the invention show obviously compared with
The high tolerance to naphthalene and the degradation rate to naphthalene.
Embodiment 2
The present embodiment for illustrate serratia marcescens provided by the invention in inorganic salts water phase to the tolerance of naphthalene and
Degradability
By bacterial strain QD-G containing concentration to be respectively 10mg/L, 20mg/L after the access sterilizing of the inoculum concentration of 1 volume %,
In the MSM minimal medium of the naphthalene of 50mg/L, 100mg/L, 200mg/L.It is cultivated for 24 hours under the conditions of 170rpm, 30 ± 1 DEG C.
The results show that bacterial strain QD-G shows good tolerance to naphthalene using naphthalene as sole carbon source.Thallus is attainable
Maximum OD value and 2 are shown in Table to the degradation rate of naphthalene.
Comparative example 2-1
This comparative example is used to illustrate the bacterial strain D of reference in inorganic salts water phase to the tolerance of naphthalene and degradability
The MSM minimal medium containing different naphthalene concentration is prepared according to the method for embodiment 2 and it is handled, no
With the bacterial strain of access is reference strains D.The attainable maximum OD value of thallus and 2 are shown in Table to the degradation rate of naphthalene.
Comparative example 2-2
This comparative example is for illustrating under natural conditions in inorganic salts water phase to the degradability of naphthalene (blank control)
The MSM minimal medium containing different naphthalene concentration is prepared according to the method for embodiment 2 and it is handled, no
It is same, it is not inoculated with any strain, but same amount of inorganic salts culture solution is added.The maximum OD value of culture solution and the drop of naphthalene
Solution rate is shown in Table 2.
Table 2
As can be seen from Table 2, in the MSM minimal medium using naphthalene as sole carbon source, compared with bacterial strain D, the present invention
Bacterial strain QD-G show the significantly higher tolerance to naphthalene and the degradation rate to naphthalene.
Embodiment 3
The present embodiment for illustrate serratia marcescens provided by the invention in inorganic salts water phase to the tolerance of naphthalene and
Degradability
By bacterial strain QD-G with 100ml after the access sterilizing of the inoculum concentration of 1 volume % contain concentration for 100mg/L containing naphthalene without
Machine salt culture medium (on the basis of 1L minimal medium, the NH containing 10g4NO3, 1.5g KH2PO4, 3g K2HPO4、
The MgSO of 0.008g4, 0.002 CuSO4, 0.002 MnSO4, 0.002 FeSO4, 0.002 CaCl2, pH=7.5) in.?
170rpm cultivates 3d under the conditions of 35 ± 1 DEG C.Bacterial strain QD-G is 97% to the degradation rate of naphthalene.
Embodiment 4
The present embodiment is for illustrating serratia marcescens provided by the invention in the soil to the degradability of naphthalene
Bacterial strain QD-G is inoculated into the LB liquid medium of 100mL with 1 volume % strain amount, 175rmp, at 30 ± 1 DEG C
After culture for 24 hours, bacterium solution is added to 5kg and is contained in the contaminated soil of 20mg/kg naphthalene, is stirred evenly, cultivated 7 days, guaranteed containing soil
Water in earth is 20% or so, finally detects the content of naphthalene in soil, the results are shown in Table 3.
Comparative example 4-1
This comparative example is used to illustrate the bacterial strain D of reference in the soil to the tolerance of naphthalene and degradability
Soil is handled according to the method for embodiment 4, unlike, the bacterial strain of access is reference strains D.Finally examine
The content for surveying naphthalene in soil, the results are shown in Table 3.
Comparative example 4-2
This comparative example is used to illustrate under natural conditions the degradability (blank control) of naphthalene in the soil
Soil is handled according to the method for embodiment 4, unlike, same amount of LB liquid medium is only added.
The content for finally detecting naphthalene in soil, the results are shown in Table 3.
Embodiment 5
The present embodiment is for illustrating serratia marcescens provided by the invention in the soil to the degradability of naphthalene
Bacterial strain QD-G is inoculated into the LB liquid medium of 100mL with 1 volume % strain amount, 175rmp, at 30 ± 1 DEG C
After culture for 24 hours, bacterium solution is added in the naphthalene contaminated soil that 5kg contains 20mg/kg, is stirred evenly, and is added 10mL's daily
MSM minimal medium is cultivated 7 days, is guaranteed containing the water in soil to be 20% or so, is finally detected the content of naphthalene in soil,
It the results are shown in Table 3.
Embodiment 6
The present embodiment is for illustrating serratia marcescens provided by the invention in the soil to the degradability of naphthalene
Bacterial strain QD-G is inoculated into the LB liquid medium of 100mL with 1 volume % strain amount, 175rmp, at 30 ± 1 DEG C
After culture for 24 hours, bacterium solution is centrifuged, removes supernatant, and thallus is washed with deionized to be added to after 3 times and is contained containing 5kg
It in the contaminated soil of the naphthalene of 20mg/kg, stirs evenly, cultivates 7 days, guarantee containing the water in soil to be 20% or so, finally examine
The content for surveying naphthalene in soil, the results are shown in Table 3.
Table 3
| Number | Embodiment 4 | Comparative example 4-1 | Comparative example 4-2 | Embodiment 5 | Embodiment 6 |
| Naphthalene content (mg/kg) | 13.3 | 18.4 | 19.8 | 7.26 | 2.4 |
As can be seen from Table 3, compared with the bacterial strain D belonged to, serratia marcescens A provided by the invention is to the naphthalene in soil
It is able to carry out effective degradation.In addition, in the case where being preferably added to inorganic salts or being added in soil in the form of pure thallus,
Serratia marcescens A provided by the invention can be further enhanced to the degradation rate of naphthalene.
Embodiment 7
It is biological prosthetic to the progress of naphthalene contaminated soil according to following 5 kinds of modes, naphthalene is added in every part of 600g soil and (is dissolved in just
Hexane), make 0.5 weight % of naphthalene content.
1, it sterilizes blank control group (C): by soil loaded on the 15min that sterilized in conical flask at 121 DEG C, totally 2 times；
2, Natural Attenuation control group (N): without any processing；
3, biological reinforced group (B): taking bacteria suspension to be centrifuged, and it is 1.0 that OD600 value is adjusted to after being resuspended with physiological saline, takes
30mL is added in soil；
4, biostimulation group (S): being that 100:1.25:1 is added into soil containing KH according to N:P2PO4、NH4NO3Nutrient solution
30mL；
5, biostimulation+biological reinforced group (BS): into soil simultaneously add resuspended bacterium solution 30mL and the 3rd group described in battalion
Nutrient solution 30mL.
Soil system after above-mentioned 5 kinds of different disposals is placed in 25 DEG C of artificial climate incubators, as experiment after staying overnight
Starting point, stirring soil daily and deionized water is added makes soil moisture content (20 ± 2) %.The reparation operating time is 31d, soil
Naphthalene removal rate changes as shown in Figure 1 with repair time in earth.
As shown in Figure 1, naphthalene removal rate is 50.03% in sterilizing control group (C).Final naphthalene removal rate is in N group
54.64%.The removal rate of naphthalene is 62.22% in S group.And after degradation bacteria strains QD-G is added, it is handled by 31d, naphthalene in B group
Removal rate is 71.94%.Final naphthalene removal rate is 83.14% in BS group.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, it can be combined in any appropriate way.In order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (8)
1. one plant of serratia marcescens (Serratia marcescens), which is characterized in that the preservation of the serratia marcescens
Number is CGMCC No.10847.
2. a kind of microbial inoculum, which is characterized in that the microbial inoculum contains serratia marcescens (Serratia described in claim 1
marcescens)。
3. serratia marcescens described in claim 1 and/or microbial inoculum as claimed in claim 2 answering in degrading polycyclic aromatic hydrocarbons
With；
Wherein, the polycyclic aromatic hydrocarbon is naphthalene.
4. a kind of method of degrading polycyclic aromatic hydrocarbons, this method comprises: by serratia marcescens described in claim 1
(Serratia marcescens) and/or microbial inoculum as claimed in claim 2 are contacted with the environment containing polycyclic aromatic hydrocarbon, to ring
Polycyclic aromatic hydrocarbon in border is degraded；
Wherein, the polycyclic aromatic hydrocarbon is naphthalene.
5. according to the method described in claim 4, wherein, the environment containing polycyclic aromatic hydrocarbon includes soil or water body.
6. method according to claim 4 or 5, wherein this method further includes, in the process to the degrading polycyclic aromatic hydrocarbons
In, inorganic salts are added into the environment containing polycyclic aromatic hydrocarbon.
7. according to the method described in claim 6, wherein, the inorganic salts are selected from KH2PO4、K2HPO4、NH4NO3、MgSO4、
CaCl2And FeSO4One of or it is a variety of.
8. method according to claim 4 or 5, wherein claim 1 is added into the environment containing polycyclic aromatic hydrocarbon
The serratia marcescens (Serratia marcescens), the serratia marcescens of addition by through Liquid Culture simultaneously
Solid phase after separation of solid and liquid and/or the bacterium colony after solid culture provide.
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| CN110804567B (en) * | 2019-11-14 | 2021-03-02 | 重庆理工大学 | Serratia marcescens capable of degrading chlorobenzene and application thereof |
| CN111394284B (en) * | 2020-04-28 | 2021-10-15 | 河北萌帮水溶肥料股份有限公司 | Serratia marcescens MB21 and application thereof |
| CN113800647A (en) * | 2020-10-16 | 2021-12-17 | 西安工程大学 | Application of serratia marcescens strain in decolorizing and degrading vat blue RSN anthraquinone dye |
| CN114891668B (en) * | 2022-04-27 | 2024-02-23 | 华中农业大学 | Serratia marcescens strain for degrading polycyclic aromatic hydrocarbon pollutants and application thereof |
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