CN102674560A - Process for biodegrading yellow water of white spirit factory - Google Patents

Process for biodegrading yellow water of white spirit factory Download PDF

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CN102674560A
CN102674560A CN2012101696815A CN201210169681A CN102674560A CN 102674560 A CN102674560 A CN 102674560A CN 2012101696815 A CN2012101696815 A CN 2012101696815A CN 201210169681 A CN201210169681 A CN 201210169681A CN 102674560 A CN102674560 A CN 102674560A
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yellow water
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aspergillus oryzae
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CN102674560B (en
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张志才
任晓锋
刘丹
申王莉
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Jiangsu University
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Abstract

The invention discloses a process for biodegrading yellow water of a white spirit factory, and relates to the technical field of bioengineering. According to the process, aspergillus oryzae is utilized as a starting strain, and the COD (Chemical Oxygen Demand) of the yellow water of the spirit factory is reduced through a biological fermentation process. The process comprises the flow consisting of the following steps of: inoculating the aspergillus oryzae; performing shake-flask culture and first-stage seed culture; inoculating into diluted yellow water of the white spirit factory; and fermenting and culturing. By using the method, the COD removal rate can reach 60-98 percent, the BOD (Biochemical Oxygen Demand) removal rate reaches 90-99 percent, the pH is increased from original 3.5 to 7.0-8.0, the produced wastewater is subjected to centrifugation and molecular interception, and thalli and active molecules serving as feed additives can be obtained.

Description

The technology of a kind of biological degradation distillery yellow water
Technical field
The present invention relates to technical field of bioengineering, refer in particular to and utilize aspergillus oryzae degraded brewery yellow water.Insert the COD in the aspergillus oryzae bacterial classification degrading waste water with after 3~10 times of the former brewery yellow water dilutions.After the aspergillus oryzae degraded, the waste water COD clearance can reach 60~98%, and the BOD clearance reaches 90~99%, and pH rises to 7.0 by original pH, and the waste water after degrading simultaneously can extract mycelium and activeconstituents is used as fodder additives.
Background technology
Liquor has the traditional history above thousand in China, and in recent years, along with developing rapidly of liquor industry, its environmental problem of bringing is also serious day by day.Although the existing certain time limit of liquor factory effluent, the situation and pessimistic on the whole of administering explored by China.Domestic existing solid state fermentation liquor-making enterprises fully utilizes the yellow water that zymotechnique partly produces, and its organic concentration is higher, pollutes very big; If directly discharging not only causes the great wasting of resources, and surrounding enviroment are caused have a strong impact on.
At present, the domestic brewed spirit yellow water that utilizes again mainly contains following several kinds of modes: the one, yellow water directly is used for seasoning after deposition, filtration, decolouring.The 2nd, utilize behind yellow water, wine tail, koji powder, the edible ethanol proportional mixing and steam Titian through string.The 3rd, utilize the enzyme esterification technology that yellow water is carried out esterification, compositions such as organic acid are converted into the mixed solution of liquor flavor compositions such as ester class, the esterifying liquid for preparing can be gone here and there the steaming Titian, irritates the cellar for storing things, can improve the content of fragrance matter in the basic wine.The 4th, yellow water is used to maintain Jiao Chi.Yet these processing modes though can promote total ester, total acid content and fragrance, can only reclaim the lower boiling material in the yellow water; Utilization ratio is lower; And the processing cycle is longer, and the waste liquid after the esterification is still treated further processing, still will be in the face of the reality of direct exhaust emission environment.So still need yellow water is carried out advanced treatment, thoroughly solve the pollution problem of yellow water.
In addition, the color removal of malting effluent is a difficult problem, particularly vinasse waste liquid, and after anaerobic treatment, waste water is chocolate liquor factory effluent deep treatment method to be had: absorption method, membrane filter method, catalytic oxidation, coagulant sedimentation etc.Absorption method is used gac always, flyash, and husky filters etc. are sorbent material.Employing Fenton reagent such as Zhou Xiaobo carry out catalyzed oxidation to the water outlet that the alcohol waste water biological process is handled, and the waste water COD cr clearance after the processing is 60%.Chen Lichun adopts ozone oxidation method advanced treatment alcohol waste water, through the strong oxidizing property of ozone, removes hardly degraded organic substance, and effluent quality is superior to general simple biological process.Adopt the ozone method to need not to add any medicament, non-secondary pollution, but when handling waste water on a large scale, cost is corresponding also can be increased.
Adopting biological reinforcing technology to handle organic wastewater poisonous, difficult degradation also receives publicity.The ways and means of biological reinforcing technology is a lot of at present, as directly adding special efficacy degraded mikrobe.Aspergillus oryzae can be secreted multiple enzyme such as proteolytic enzyme, glycase, lypase, polygalacturonase, Sumizyme PHY, saccharifying enzyme etc., so this bacterium constantly expands in the Industrial Application field in modern times.People such as Tejomyee S. B. are obtained aspergillus oryzae from separating the SD-9129 Contaminated soil, study its biological degradation to the agricultural chemicals SD-9129, and result of study is illustrated in the short period aspergillus oryzae, and degraded has high efficiency to SD-9129.People such as Truong Q.T. have studied with aspergillus oryzae and have handled the situation in the tapioca(flour) processing waste water that contains the high-concentration suspension body, improve its degradation efficiency to waste water through changing the multiple factor that influences the aspergillus oryzae growth, and its degradation rate can be up to 90%.People such as Xiang J.M. separate the aspergillus oryzae of the Nicotine that obtained degrading from tobacco leaf, the resting cell analysis of research and utilization aspergillus oryzae is to the degradation pathway of Nicotine.Through analysis, finally confirmed to utilize the new way of fungus degrading Nicotine to the degradation process intermediate product.People such as Parshetti G.K. have studied the biological degradation of aspergillus oryzae NCIM-1146 to Reactive blue-25.The decolouring of discovering Reactive blue is that the absorption through fungi just is degraded then, under the shaking table culture condition, absorbs faster and decolours than leaving standstill to cultivate to have.Improved the degraded of aspergillus oryzae after adding glucose to Reactive blue.This shows that aspergillus oryzae has the ability of degraded than hardly degraded organic substance.
The inventor is through deep research, find out a kind of with aspergillus oryzae as starting strain, the method for degradation of white spirit factory yellow water.This method is different from the past method and is that employed bacterial classification is by the aspergillus oryzae bacterial classification of the feed level microbe additive of the public directly feeding animals of deciding association (AAFCO), the identification of China Ministry of Agriculture of united States food and drug administration (FDA) and U.S. feed; Degradation of white spirit factory yellow water obtains tropina and the activeconstituents that contains one or more zymins fermented liquids such as (saccharifying enzyme, proteolytic enzyme, Sumizyme PHY, lypase) simultaneously.These thalline and activeconstituents can be used as fodder additives.
Summary of the invention
The present invention is to provide the biotechnology of aspergillus oryzae degraded brewery yellow water.After brewery's yellow water was degraded through aspergillus oryzae, its pH obviously was increased to alkalescence, and the COD clearance can reach 60~98%, and the BOD clearance reaches 90~99%.
The technology of degraded yellow water of the present invention; Carry out according to following step: be starting strain with the aspergillus oryzae; Carry out test tube enlarged culturing, liquid shaking bottle cultivation, first order seed cultivation and degraded yellow water; Waste water after the degraded is through the aspergillus oryzae filament of centrifugal acquisition, and filtrating obtains multiple bioactive molecules through molecular retention, and these mycelium and bioactive molecules can be used in the fodder additives.
The used bacterial classification of the present invention is any bacterial classification of aspergillus oryzae, like the bacterial classification of Chinese common micro-organisms culture presevation administrative center (CCGMC), Chinese agriculture microbial strains preservation administrative center (ACCC), Chinese industrial microbial strains preservation administrative center (CICC), Chinese medicine microbial strains preservation administrative center (CMCC), national veterinary microorganism DSMZ (CVCC) and microbiotic bacterial classification preservation administrative center or the like preservation.
Wherein said test tube enlarged culturing base is the potato sucrose substratum.
The substratum that wherein said liquid shaking bottle is cultivated and first order seed is cultivated is (grams per liter): glucose 5~30, ammonium tartrate 0.1~5, phenylcarbinol 0.2~3; Sal epsom 0.2~1; Tween 80 0.5~10, potassium primary phosphate 2~9, phthalic acid 3~18 grams; PH 5~8, sterilize 20~40 minutes for 120~140 ℃;
Wherein said shake-flask culture processing condition do, inoculation one ring aspergillus oryzae test tube slant spore is in the triangular flask of the shake-flask culture base that 16~48% (volume ratios) are housed, at rotating speed: 150 rev/mins, 25~35 ℃ of temperature were cultivated 24~72 hours;
Wherein said first order seed culture process does; Inoculum size by 1~20% (volume ratio) is inoculated into the shake-flask culture seed liquor in the first order seed substratum; 25~35 ℃ of temperature; 50~200 rev/mins of mixing speed, air flow 0.2~2:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 18~72 hours;
Wherein said degraded yellow water technology is: the consisting of of substratum: 0.5~3 kilogram of glucose, 0.5~2 kilogram of soybean cake powder; Copper sulfate 5~15 grams, ferrous sulfate 5~15 grams, manganous sulfate 5~20 grams, above-mentioned raw materials joins yellow water 1000L (the yellow water original C OD of 3~10 times of dilutions CR=35000mg/L); Degradation technique is following: by 2~20% (seed liquor volume/wastewater volume) inoculation level liquid bacterial classification; 25~35 ℃ of temperature; 50~200 rev/mins of mixing speed; Air flow 0.2~2:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 36~168 hours.
Each composition of substratum in the wherein above-mentioned degraded yellow water technology can amplify in this ratio;
Wherein said yellow water is the yellow water of any one liquor by solid fermentation factory, like five-Grain Liquor, Maotai, the Yanghe River, this life liquor such as edge.
After using this spent process water degraded, the COD clearance can reach 60~98%, and the BOD clearance reaches 90~99%, and pH rises to 7.0 by original pH.
According to fermentating culturing process of the present invention, in conjunction with the ABC of this area, can increase secondary, three grades even level Four seeding tank according to the production needs, further enlarge the solid state fermentation industrial scale, to carry out suitability for industrialized production.The substratum that secondary, three grades even level Four seeding tank adopt is identical with the first order seed substratum with the shake-flask culture base, and inoculum size is 5~20%, and culture condition is same as the first order seed cultivation,
Waste water after the wherein said degraded is through the aspergillus oryzae filament of centrifugal acquisition; Filtrating obtains multiple bioactive molecules through molecular retention; Carry out according to following step: with brewery's yellow water of above-mentioned aspergillus oryzae degraded, centrifugal 5~20 minutes of 3000~6000rpm obtains mycelium; The mycelium oven dry is preserved; Filtrating is held back concentrated 50~100 times through the film of molecular weight 5000~10000, and-20 ℃ of lyophilizes obtain macromolecular activeconstituents.
This art breading brewery yellow water advantage is that the bacterial classification that adopts is the directly aspergillus oryzae of the feed level microbe additive of feeding animals that the Ministry of Agriculture is assert; Therefore obtain thalline and zymin can be used as fodder additives; Make yellow water be able to resource utilization, reduced the cost of wastewater treatment.
Description of drawings
Fig. 1 is the process flow sheet of this patent.
Embodiment
In order further to set forth related material and the technology of technical scheme of the present invention, provided following examples, but these embodiment do not limit scope of the present invention in any form.
The COD that the present invention adopts CRMeasuring method is: accurately pipette 10mL water sample and 90mL zero(ppm) water in the 250mL Erlenmeyer flask, add 10mL H 2SO 4(1:3), and accurately add 10.00mL 0.002 mol/L KMnO 4Solution is put into boiling water bath heating 30 minutes (heat-processed answers appropriate supplement to add potassium permanganate solution if observing redness takes off) with Erlenmeyer flask, and the water-bath liquid level will be higher than the liquid level in the Erlenmeyer flask, makes fully oxidation of reducing substances quilt wherein.After the taking-up, solution should be light red, accurately adds 0.005mol/L Na immediately 2C 2O 4Standardized solution 10.00mL, redness should be decorporated fully.Descend with potassium permanganate solution titration to blush the colour-fast terminal point (solution temperature should not be lower than 60oC during terminal point) that is in 30 seconds then at 70~80 ℃.Record potassium permanganate solution consumption.Replace water sample to repeat the amount of above-mentioned experimental record potassium permanganate with 10mL zero(ppm) water, as blank test.COD calculates according to formula:
Wherein: V 1: potassium permanganate solution consumption (mL) during the titration water sample; V: potassium permanganate solution consumption during blank titration; M: the concentration of potassium permanganate solution.
Embodiment 1
1, the making of test tube slant bacterial classification
Potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook " in new configuration; 1994; 367 pages) in insert a ring aspergillus oryzae bacterial classification (Aspergillus oryzae) (Chinese common micro-organisms culture presevation administrative center; CGMCC5992), 26 ℃, incubation time 50 hours; 4 ℃ of preservations are subsequent use.
2, the making of liquid shaking bottle cultured strain
Accurately take by weighing glucose 5 grams, ammonium tartrate 0.1 gram, phenylcarbinol 0.2 gram, sal epsom 0.2 gram, tween 80 0.5 gram; Potassium primary phosphate 2 grams, O-phthalic acid buffer 3 grams, water 1000 mL, pH 5; Packing 250mL triangular flask, every bottle of 50mL, sterilized 40 minutes for 120 ℃ totally by 20 bottles; After the cooling, every bottle graft is gone into the aspergillus oryzae bacterial classification of 4 ℃ of preservations of a ring, at 25 ℃, 150 rev/mins, cultivates 72 hours;
3, the making of level liquid bacterial classification
Accurately take by weighing glucose 50 grams, ammonium tartrate 1 gram, phenylcarbinol 2 restrains, sal epsom 2 grams, tween 80 5 grams, potassium primary phosphate 20 grams, phthalic acid 30 grams, water 10L is loaded in the seeding tank of 15L, sterilizes 20 minutes for 120 ℃; After the sterilization cooling, 25 ℃ of temperature, 50 rev/mins of mixing speed, 0.2: 1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) of air flow was cultivated 72 hours;
4, yellow water degraded
Dilute 3 times the yellow water 1000L of the Yanghe River, Jiangsu brewery, add 0.5 kilogram of glucose, 0.5 kilogram of soybean cake powder; Copper sulfate 5 grams, ferrous sulfate 5 grams, manganous sulfate 5 grams; Insert 2% (seed liquor volume/wastewater volume) inoculation level liquid bacterial classification; 25 ℃ of temperature, 60 rev/mins of mixing speed, air flow 0.3:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM); Cultivate after 44 hours; The waste water COD clearance can reach 65%, and the BOD clearance reaches 90%, and pH rises to 7.0 by original pH.
5, thalline and active substance extract
The distillery waste of above-mentioned aspergillus oryzae degraded, centrifugal 6 minutes of 3000rpm obtains mycelium, and the mycelium oven dry is preserved.Filtrating is held back concentrated 50 times through the film of molecular weight 5000, and-20 ℃ of lyophilizes obtain macromolecular activeconstituents.This activeconstituents contains following one or more enzymes: like saccharifying enzyme, proteolytic enzyme, Sumizyme PHY, lypase.4.9 kilograms of the outer activeconstituents gross weights of thalline and born of the same parents.(punishment comes monarch owing to many documents; Common mycology; 1999) report; Therefore multiple enzymes such as aspergillus oryzae can extracellular proteinase, glycase, lypase, polygalacturonase, Sumizyme PHY, saccharifying enzyme infer that this activeconstituents contains following one or more enzymes: like saccharifying enzyme, proteolytic enzyme, Sumizyme PHY, lypase.2~5 kilograms of the outer activeconstituents gross weights of thalline and born of the same parents.
Embodiment 2
1, the making of test tube slant bacterial classification
In the potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook ", 1994,367 pages) of new configuration, insert a ring aspergillus oryzae bacterial classification (Aspergillus oryzae), 30 ℃, incubation time 100 hours; 7 ℃ of preservations are subsequent use.
2, the making of liquid shaking bottle cultured strain
Accurately take by weighing glucose 150 grams, ammonium tartrate 250 grams, phenylcarbinol 15 grams, sal epsom 0.4 gram, tween 80 4 grams; Potassium primary phosphate 5 grams, phthalic acid buffering 20 grams, water 10 L, pH 6; Packing 250mL triangular flask, every bottle 100 gram, was sterilized 30 minutes for 130 ℃ totally by 100 bottles; After the cooling, every bottle graft is gone into the aspergillus oryzae bacterial classification of 7 ℃ of preservations of a ring, at 30 ℃, 150 rev/mins, cultivates 50 hours;
3, the making of level liquid bacterial classification
Accurately take by weighing glucose 2000 grams, ammonium tartrate 200 grams, phenylcarbinol 150 restrains, sal epsom 60 grams, tween 80 60 grams, potassium primary phosphate 400 grams, phthalic acid 1000 grams, water 100L is loaded in the seeding tank of 130L, sterilizes 30 minutes for 130 ℃; After the cooling, 30 ℃ of temperature, 125 rev/mins of mixing speed, air flow 1:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 50 hours;
4, yellow water degraded
Dilute the yellow water 1000L of Jiangsu King's Luck Brewery Co., Ltd. of 7 times, 2 kilograms of glucose, 1.2 kilograms of soybean cake powders; Copper sulfate 10 grams, ferrous sulfate 10 grams, manganous sulfate 12 grams; By 13% (seed liquor volume/wastewater volume) inoculation level liquid bacterial classification, 30 ℃ of temperature, 120 rev/mins of mixing speed, air flow 1:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 100 hours.This moment, the COD clearance can reach 89%, and the BOD clearance reaches 95%, and pH rises to 7.0 by original pH.
5, the preparation of thick enzyme prepn
The distillery waste of Jiangsu King's Luck Brewery Co., Ltd. of aspergillus oryzae degraded, centrifugal 15 minutes of 4500rpm obtains mycelium, and the mycelium oven dry is preserved.Filtrating is held back concentrated 70 times through the film of molecular weight 8000, and-20 ℃ of lyophilizes obtain macromolecular activeconstituents.This activeconstituents contains following one or more enzymes: like saccharifying enzyme, proteolytic enzyme, Sumizyme PHY, lypase.3.5 kilograms of the outer activeconstituents gross weights of thalline and born of the same parents.
 
Embodiment 3
1, the making of test tube slant bacterial classification
In the potato sucrose substratum (Zhu Gejian, Wang Zhengxiang chief editor's " industrial microorganism experimental technique handbook ", 1994,367 pages) of new configuration, insert a ring aspergillus oryzae bacterial classification (Aspergillus oryzae), 35 ℃, incubation time 144 hours; 10 ℃ of preservations are subsequent use.
2, the making of liquid shaking bottle cultured strain
Accurately take by weighing glucose 300 grams, ammonium tartrate 50 grams, phenylcarbinol 30 grams, sal epsom 10 grams, tween 80 100 grams; Potassium primary phosphate 90 grams, O-phthalic acid buffer 180 grams, water 10L, pH7.5; Packing 250mL triangular flask, every bottle 120 mL, sterilized 20 minutes for 140 ℃ totally by 80 bottles; After the cooling, every bottle graft is gone into the aspergillus oryzae bacterial classification of 10 ℃ of preservations of a ring, at 35 ℃, 150 rev/mins, cultivates 72 hours;
3, the making of level liquid bacterial classification
Accurately take by weighing glucose 1500 grams, ammonium tartrate 250 grams, phenylcarbinol 150 restrains, sal epsom 50 grams, tween 80 500 grams, potassium primary phosphate 450 grams, O-phthalic acid buffer 900 grams, water 50L is loaded in the first class seed pot of 70L, sterilizes 40 minutes for 140 ℃; After the sterilization cooling, 35 ℃ of temperature, 200 rev/mins of mixing speed, air flow 2:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 50 hours;
4, the making of second-class liquid isolate
Accurately take by weighing glucose 15000 grams, ammonium tartrate 2500 grams, phenylcarbinol 1500 grams, sal epsom 500 grams; Tween 80 5000 grams, potassium primary phosphate 4500 grams, O-phthalic acid buffer 9000 grams; Water 500L is loaded in the first class seed pot of 700L, sterilizes 40 minutes for 140 ℃; After the sterilization cooling, 35 ℃ of temperature, 200 rev/mins of mixing speed, air flow 2:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 18 hours;
5, yellow water degraded
Raw material consists of the yellow water 1000L of 10 times of dilutions, 3 kilograms of glucose, 1.8 kilograms of soybean cake powders; Copper sulfate 15 grams; Ferrous sulfate 15 grams, manganous sulfate 20 grams are by 20% (seed liquor volume/wastewater volume) inoculation level liquid bacterial classification; 34 ℃ of temperature; 190 rev/mins of mixing speed, air flow 2:1 volume/PM (ventilation gas volume/fermentating liquid volume/PM) was cultivated 168 hours.The COD clearance can reach 97%, and the BOD clearance reaches 99%, and pH rises to 7.0 by original pH.
6, the preparation of thick enzyme prepn
The distillery waste of aspergillus oryzae degraded, centrifugal 20 minutes of 6000rpm obtains mycelium, and the mycelium oven dry is preserved.Filtrating is held back concentrated 100 times through the film of molecular weight 10000, and-20 ℃ of lyophilizes obtain macromolecular activeconstituents.This activeconstituents contains following one or more enzymes: like saccharifying enzyme, proteolytic enzyme, Sumizyme PHY, lypase.2.4 kilograms of the outer activeconstituents gross weights of thalline and born of the same parents.

Claims (9)

1. the technology of a biological degradation distillery yellow water; It is characterized in that carrying out: be starting strain with the aspergillus oryzae according to following step; Carry out test tube enlarged culturing, liquid shaking bottle cultivation, first order seed cultivation and degraded yellow water; Waste water after the degraded is through the aspergillus oryzae filament of centrifugal acquisition, and filtrating obtains multiple bioactive molecules through molecular retention.
2. the technology of a kind of biological degradation according to claim 1 distillery yellow water; It is characterized in that used bacterial classification is any bacterial classification of aspergillus oryzae, like the bacterial classification of Chinese common micro-organisms culture presevation administrative center (CCGMC), Chinese agriculture microbial strains preservation administrative center (ACCC), Chinese industrial microbial strains preservation administrative center (CICC), Chinese medicine microbial strains preservation administrative center (CMCC), national veterinary microorganism DSMZ (CVCC) and microbiotic bacterial classification preservation administrative center or the like preservation.
3. the technology of a kind of biological degradation according to claim 1 distillery yellow water is characterized in that wherein said test tube enlarged culturing base is the potato sucrose substratum.
4. the technology of a kind of biological degradation according to claim 1 distillery yellow water is characterized in that the substratum that wherein said liquid shaking bottle is cultivated with the first order seed cultivation is (grams per liter): glucose 5~30, ammonium tartrate 0.1~5; Phenylcarbinol 0.2~3, sal epsom 0.2~1, tween 80 0.5~10; Potassium primary phosphate 2~9; Phthalic acid 3~18 grams, pH 5~8, sterilize 20~40 minutes for 120~140 ℃.
5. the technology of a kind of biological degradation according to claim 1 distillery yellow water; It is characterized in that wherein said shake-flask culture processing condition do; Inoculation one ring aspergillus oryzae test tube slant spore is in the triangular flask of the shake-flask culture base that 16~48% (volume ratios) are housed; At rotating speed: 150 rev/mins, 25~35 ℃ of temperature were cultivated 24~72 hours.
6. the technology of a kind of biological degradation according to claim 1 distillery yellow water; It is characterized in that wherein said first order seed culture process does; Inoculum size by 1~20% (volume ratio) is inoculated into the shake-flask culture seed liquor in the first order seed substratum, 25~35 ℃ of temperature, and 50~200 rev/mins of mixing speed; Air flow 0.2~2:1 volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivated 18~72 hours.
7. the technology of a kind of biological degradation according to claim 1 distillery yellow water is characterized in that wherein said degraded yellow water technology is: the consisting of of substratum: 0.5~3 kilogram of glucose, 0.5~2 kilogram of soybean cake powder; Copper sulfate 5~15 grams, ferrous sulfate 5~15 grams, manganous sulfate 5~20 grams, above-mentioned raw materials joins yellow water 1000L (the yellow water original C OD of 3~10 times of dilutions CR=35000mg/L); Degradation technique is following: by 2~20% (seed liquor volume/wastewater volume) inoculation level liquid bacterial classification; 25~35 ℃ of temperature; 50~200 rev/mins of mixing speed, air flow 0.2~2:1 volume/minute (ventilation gas volume/fermentating liquid volume/minute), cultivated 36~168 hours.
8. the technology of a kind of biological degradation according to claim 1 distillery yellow water is characterized in that wherein said yellow water is the yellow water of any one liquor by solid fermentation factory, like five-Grain Liquor, Maotai, the Yanghe River, this life liquor such as edge.
9. the technology of a kind of biological degradation according to claim 1 distillery yellow water; It is characterized in that waste water after the wherein said degraded through the aspergillus oryzae filament of centrifugal acquisition, filtrating obtains multiple bioactive molecules through molecular retention, carries out according to following step: brewery's yellow water of aspergillus oryzae degraded that will be above-mentioned; Centrifugal 5~20 minutes of 3000~6000rpm; Obtain mycelium, the mycelium oven dry is preserved; Filtrating is held back concentrated 50~100 times through the film of molecular weight 5000~10000, and-20 ℃ of lyophilizes obtain macromolecular activeconstituents.
CN201210169681.5A 2012-05-29 2012-05-29 Process for biodegrading yellow water of white spirit factory Expired - Fee Related CN102674560B (en)

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CN103466804A (en) * 2013-09-09 2013-12-25 江苏大学 Technique for removing anthraquinone compounds in wastewater by using Aspergillus oryzae thallus
CN103466807A (en) * 2013-09-09 2013-12-25 江苏大学 Technique for degrading anthraquinone compounds by using Ceriporiopsis subvermispora
CN103466804B (en) * 2013-09-09 2016-04-06 江苏大学 Aspergillus oryzae cell is utilized to remove anthraquinone analog compound Technology in waste water
CN103466806B (en) * 2013-09-09 2016-04-06 江苏大学 Gallic acid Technology in a kind of aspergillus oryzae degrading waste water
CN103663721A (en) * 2013-09-10 2014-03-26 江苏大学 Method for degrading gallic acid in wastewater by using Phanerochaete chrysosporium
CN103663720A (en) * 2013-09-10 2014-03-26 江苏大学 Technique for removing gallic acid in wastewater by using Phanerochaete chrysosporium thallus
CN103663720B (en) * 2013-09-10 2015-10-28 江苏大学 Phanerochaete chrysosporium thalline is utilized to remove gallic acid Technology in waste water
CN103663718A (en) * 2013-09-10 2014-03-26 江苏大学 Technique for removing gallic acid in wastewater by using Aspergillus oryzae thallus
CN103663719A (en) * 2013-09-10 2014-03-26 江苏大学 Technique for removing gallic acid in wastewater by using Ceriporiopsis subvermispora thallus
CN103663718B (en) * 2013-09-10 2016-07-13 江苏大学 Aspergillus oryzae cell is utilized to remove gallic acid Technology in waste water
CN108504593A (en) * 2018-03-27 2018-09-07 丽水学院 A kind of compound microbial preparation, preparation method and its application in sewage disposal
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