CN103894400A - Method for promoting enrichment of cadmium of red spinach by using plant growth regulator - Google Patents
Method for promoting enrichment of cadmium of red spinach by using plant growth regulator Download PDFInfo
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Abstract
The invention discloses a method for promoting enrichment of cadmium of red spinach by using a plant growth regulator. The method is characterized by comprising the steps: planting red spinach in soil containing a pollutant cadmium, spraying the plant growth regulator, and when the plant red spinach grows until a flowering period or a mature period, harvesting or integrally removing the plant red spinach from polluted soil; further planting the next batch of red spinach, spraying the plant growth regulator, when the plant red spinach grows until the flowering period or the mature period, integrally removing the plant red spinach from the polluted soil, and repeating the process until the content of the cadmium in the soil reaches the environment safety standard. By adopting the method, the absorption, transfer and enrichment of the red spinach to cadmium can be remarkably increased, the plant remediation efficiency of the cadmium polluted soil is effectively increased, the method for remediating the cadmium polluted soil has the characteristics of less investment, low maintenance cost, small work amount, low management technical requirement, and the like, and better environment benefit, social benefit and economic benefit are achieved.
Description
Technical field
The invention belongs to the regeneration of cadmium pollution improvement and contaminated soil, relate to a kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium.Be applicable to utilize plant growth regulator (or claiming plant hormone) to promote red three-coloured amaranth to repair cadmium (Cd) contaminated soil, thus repairing and treating contaminated soil.
Background technology
At present, Cd is classified as the first carcinogenic substance by international cancer research institution, and Cd is that toxicity is the strongest, pollutes one of the most serious heavy metal.Cadmium in Soil content is 0.01～2mg/kg, is mainly derived from soil parent material, have cadmium mineralization occur and by the place of industrial pollution, Cadmium in Soil content can exceed over one hundred times of background value, have up to thousands of times.The toxic action of contaminated by heavy metals soil environment to crops, its impact is: be exactly some tissues and the function of destroying plant on the one hand, thereby reduce the yield and quality of plant, for example Cadmium in Soil content overproof can destroy the chlorophyll structure of plant leaf blade and finally cause plant decline; Heavy metal element toxicity is large, difficult degradation, can directly act on human body by drinking water or domestic water after entering water body environment, also can be absorbed by aquatic animals and plants enrichment, enters food chain and endangers person poultry safety.Cadmium, after food chain enters human body, is mainly accumulated in liver, kidney, pancreas, thyroid gland and bone, can cause Itai-itai diseases.In addition, cadmium also can cause the illnesss such as anaemia, hypertension, ephritis and endocrinopathy.The acute poisoning main manifestations of cadmium is that respiratory system is impaired, and the slow poisoning of cadmium is mainly to cause renal tubule pathology.
For heavy metal pollution, phytoremediation technology becomes the focus of scientist research, compares with traditional processing method, has small investment, easy to operate, the feature that do not cause secondary pollution, safety.And research screening hyperaccumulative plant has become basis and the key problem of phytoremediation technology.External heavy metal super-enriched plant screening operation starts early, and comparison of results is abundant.In China, the screening study starting of super enriching plant is more late, has also obtained in recent years a series of achievements.In the reparation research to heavy metal Cd, the yellow Cd that can first-class research have found that a kind of dry land willow strain can enriched, the highest enriching quantity can reach 47.19mg/kg.Su Dechun etc. have carried out the test of resistance to Cd poison and enrichment Cd ability to multiple leaf mustard kinds, find under identical condition, mustard type rape the upperground part biomass, overground part absorb that Cd measures and to the purifying rate of pollutant all apparently higher than Indian mustard.Wei Shu has carried out the research of enriched character with waiting to the each main near coal-mine weeds of plumbous zinc ore, finds that these weeds aerial parts are all greater than 1 to the concentration coefficient of Cd; They have also found that black nightshade is Cd super enriching plant first, and the concentration coefficient of its overground part Cd is 2.68.The discoveries such as Liu Wei, under industrialized agriculture effect, Viola baoshanensis is a kind of new Cd super enriching plant, overground part Cd average content can reach 1168mg/kg under field conditions (factors), reaches as high as 2310mg/kg, and on average can reach 4825mg/kg under greenhouse experiment.
At present, plant hormone is widely used in agricultural production: seedling breeding; Improve plant output, promote solid growth, suppress or stratification flower and fruit thinning; Promote bud differentiation, suppress to nourish and grow; Regulating fruit maturation; Prevent disleave; And fresh-keeping etc. for cultivating the plant of good quality and high output and carrying out product.
Plant extract reparation refers to and utilizes the hyperaccumulative plant of certain radionuclide that the nucleic in soil is transported out, enrichment and be transported to root system of plant can absorption site and position on the ground, after plant results, process again, this Plants of plantation, can make the content of heavy metal in soil be reduced to acceptable level continuously.The mode that improves phytoremediation efficiency mainly contains at present reinforcement fertilising, adds chelating agent, improves cultivation condition, utilizes rhizosphere microorganism etc., respectively has its advantage, but also has larger limitation.Consider the dual needs of remediation efficiency and ecological, environmental protective aspect, at present, because plant regulation and control hormone in vivo level is its important way that adapts to nucleic and heavy metal stress, the research of plant hormone also becomes the focus of research gradually, especially utilize these plant hormones to resist various adverse circumstance environment, especially be applied to heavy metal, the meaning value that radionuclide is coerced aspect is great, but there is not yet the bibliographical information that utilizes plant growth regulator to promote the method for red three-coloured amaranth cadmium.
Summary of the invention
Object of the present invention is intended to overcome deficiency of the prior art, and a kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium is provided.The present invention by planting the super enriching plant-red three-coloured amaranth of cadmium in cadmium pollution soil, and apply plant growth regulator, thereby provide a kind of plant growth regulator that utilizes that can significantly increase absorption, transhipment and the enrichment of red three-coloured amaranth to cadmium to promote the method for red three-coloured amaranth cadmium, there is good environmental benefit, Social benefit and economic benefit.
Content of the present invention is: a kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, it is characterized in that comprising: containing planting red three-coloured amaranth in the soil of cadmium contaminants, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth harvesting or entirety are removed from contaminated soil.
Content of the present invention can also comprise: described red plant three-coloured amaranth entirety is removed from contaminated soil after, plant again the red three-coloured amaranth of next group, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth entirety is removed from contaminated soil, repeat said process, until the cadmium content in soil reaches environmental safety standard.
In content of the present invention: described plant growth regulator can be auxin (IAA), gibberellin (GA) or salicylic acid (SA).
Described plant growth regulator auxin (IAA) is preferably the aqueous solution that is mixed with the auxin that concentration is 100mg/L (IAA), and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
Described gibberellin of plant growth regulator (GA) is preferably the aqueous solution that is mixed with the gibberellin that concentration is 500mg/L (GA), and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
Described plant growth regulator salicylic acid (SA) is preferably the aqueous solution that is mixed with the salicylic acid that concentration is 100mg/L (SA), and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
The aqueous solution of described plant growth regulator is amount of application (spraying the amount on the blade face at red three-coloured amaranth) in every 666.7 square metres of soil: described concentration is 100mg/L(mg/litre) the amount of application of the aqueous solution of auxin preferably for 20L(liter); Described concentration is that the amount of application of the aqueous solution of the gibberellin of 500mg/L is 20L(liter preferably); Described concentration is that the amount of application of the salicylic aqueous solution of 100mg/L is 20L(liter preferably); Each amount of application also can be at 15～25L(liter) in/every 666.7 square metres of soil in.
Content of the present invention can also comprise: adopt the method for burning to carry out harmless treatment (after burning, the residual solids that contains cadmium can further adopt prior art modes such as reclaiming metal, landfill to process) harvesting or the overall red three-coloured amaranth of removing from contaminated soil.
Red three-coloured amaranth (formal name used at school: Amaranthus mangostanus L.), is Amaranthaceae Amaranthus annual herb plant, calls blue or green fragrant amaranth, three-coloured amaranth, wild thorn amaranth, rice amaranth, people Indian Rorippa Herb, apricot dish, floating heart, sparkling dish, corn dish etc.Red three-coloured amaranth is one of rich potassium plant, is mainly distributed in the nations of China and India, and its well developed root system distributes deep and broad, adaptable, and all parts of the country all can be cultivated.
The time of the sowing of described red three-coloured amaranth can be annual February to October (being spring, summer, autumn all can sow and plant); The method of plantation is sowing, and every 666.7 square metres of application rates are 0.5～0.75 kilogram; The sowing NaClO(clorox that is 0.5% with red three-coloured amaranth seed mass percent concentration) after aqueous solution soaking 15～25min, be used further to sow.
Auxin (auxin) is the endogenous hormones that a class contains a unsaturated aromatic ring and an acetic acid side chain, and the English IAA that is called for short is international, is heteroauxin (IAA).
Gibberellin (English: Gibberellin, referred to as GA) is the class plant hormone extensively existing.Its chemical constitution belongs to Diterpenes acid, is derived and is obtained by Fourth Ring skeleton.Can stimulate the growth of leaf and bud.
Salicylic acid, English name: salicylic acid, referred to as SA, claims again septichen, molecular weight 138.05, white crystals, needle-like or powdery.Proportion 1.443,156 ℃～159 ℃ of fusing points.Be dissolved in ethanol, ether, acetone, turpentine oil.
Compared with prior art, the present invention has following feature and beneficial effect:
(1) the present inventor, through long-term research, under plant growth regulator (or claiming plant hormone) is processed, can obviously improve the ability (root of red three-coloured amaranth, stem, the Cd of leaf portion content are significantly increased) of red three-coloured amaranth absorption cadmium; This improvement method expense is cheap, workable, not welding soil texture, do not cause secondary pollution;
(2) adopt the present invention, red three-coloured amaranth is cadmium (Cd) enriching plant, and have growth rapidly, biomass is large, is easy to harvesting, has the advantages such as stronger vitality; The plant growth regulator (auxin, gibberellin or salicylic acid) of choosing can improve red three-coloured amaranth biomass, cadmium uptake and cadmium accumulation ability most possibly; Thereby permanently solve Cadmium Pollution in Soils problem; The method expense is cheap, workable, not welding soil texture, do not cause secondary pollution;
(3) adopt the present invention, can make red three-coloured amaranth bring into play better absorption, the accumulation ability to cadmium, effectively improve the phytoremediation efficiency of cadmium pollution soil, cadmium pollution soil repair has small investment, maintenance cost is low, quantities is little, administrative skill requires the features such as low, has good environmental benefit, Social benefit and economic benefit.
Accompanying drawing explanation
Fig. 1 is the impact of IAA on red three-coloured amaranth individual plant accumulation cadmium;
Fig. 2 is the impact of GA on red three-coloured amaranth individual plant accumulation cadmium;
Fig. 3 is the impact of SA on red three-coloured amaranth individual plant accumulation cadmium.
The specific embodiment
Embodiment given below intends so that the invention will be further described; but can not be interpreted as it is limiting the scope of the invention; some nonessential improvement and adjustment that person skilled in art makes the present invention according to the content of the invention described above, still belong to protection scope of the present invention.
Embodiment 1:
The impact of plant growth regulator (or claiming plant hormone) on red three-coloured amaranth biomass
This test is carried out in Xinan Science and Technology Univ.'s life science and engineering college's east eight laboratory buildings, the red three-coloured amaranth seed of participating in the experiment is purchased from Mianyang Yun Chuan seeds company, and control soil is yellow soil, and soil is put in after the natural air drying of lucifuge ventilation, impurities removing, smash to pieces and sieve after for subsequent use.Sowed red three-coloured amaranth on March 19, in the time that seedling grows to 1 month, be colonizated in plastic tub (the diameter 30cm that 6.0kg soil (physicochemical property of soil is in table 2) is housed, high 23cm), wherein Cd is evenly mixed in soil take concentration as 20mg/kg aqueous solution form, continues to cultivate 1 month, and May 21 was evenly sprayed on red three-coloured amaranth blade face three plant growth regulators of variable concentrations (in table 1) with the form of the aqueous solution, according to growth needs regular watering, grow and carry out sample analysis after 140 days.
Table 1 is sprayed on the concentration of the plant growth regulator on red three-coloured amaranth blade face
Table 2 soil physical chemistry character
Red three-coloured amaranth is placed in to 105 ℃ of baking ovens 30min that completes, then at 70 ℃, dry to constant weight (drying about 48h), weigh its dry weight in the balance.
Table 3Cd processes the impact of lower auxin IAA on red three-coloured amaranth biomass
Result of the test shows: as can be known from Table 3, under Cd processes, the auxin IAA that sprays variable concentrations has obviously increased the biomass at the each position of red three-coloured amaranth, illustrates that spraying IAA can promote the growth of red three-coloured amaranth under Cd processes.In the time spraying the IAA of 100mg/L, the each position of red three-coloured amaranth biomass maximum, and ground biomass and also equal maximum of biomass of individual tree, be respectively 2.56 times and 2.59 times that contrast.
Table 4Cd processes the impact of lower gibberellin GA on red three-coloured amaranth biomass
Result of the test shows: as can be known from Table 4, under Cd processes, the gibberellin GA that sprays variable concentrations has obviously increased the biomass at the each position of red three-coloured amaranth, illustrates that spraying GA can promote the growth of red three-coloured amaranth under cadmium is processed.In the time spraying the GA of 500mg/L, the each position of red three-coloured amaranth biomass maximum, and ground biomass and also equal maximum of biomass of individual tree, be respectively 3.74 times, 3.76 times that contrast.
Table 5Cd processes the impact of lower salicylic acid SA on red three-coloured amaranth biomass
Result of the test shows: as can be known from Table 5, under Cd processes, the salicylic acid SA that sprays variable concentrations has obviously increased the biomass at the each position of red three-coloured amaranth, illustrates that spraying SA can promote the growth of red three-coloured amaranth under Cd processes.In the time spraying the SA of 100mg/L, red three-coloured amaranth root leaf site biomass maximum, and ground biomass and also equal maximum of biomass of individual tree, be respectively 2.97 times, 3.15 times that contrast.
In sum, under cadmium is processed, the plant growth regulator that sprays variable concentrations has obviously increased the biomass of red three-coloured amaranth, and 500mg/L GA process make that the ground biomass of red three-coloured amaranth and biomass of individual tree increase at most, illustrate that the facilitation that the GA of 500mg/L grows to red three-coloured amaranth is best.
Embodiment 2:
Different plant growth regulator absorb in red three-coloured amaranth body cadmium and the impact distributing
This test is carried out in Xinan Science and Technology Univ.'s life science and engineering college's laboratory building, and red three-coloured amaranth seed is purchased from Mianyang Yun Chuan seeds company, and control soil is yellow soil, soil is put in after the natural air drying of lucifuge ventilation, impurities removing, smash to pieces and sieve after for subsequent use.Sowed red three-coloured amaranth on March 19, in the time that seedling grows to 1 month, be colonizated in plastic tub (the diameter 30cm that 6.0kg soil (physicochemical property of soil is in table 2) is housed, high 23cm), wherein Cd is evenly mixed in soil take concentration as 20mg/kg aqueous solution form, continue to cultivate 1 month, May 21 was evenly sprayed on red three-coloured amaranth blade face three plant growth regulators of variable concentrations (auxin, gibberellin, salicylic acid) with the form of the aqueous solution, according to growth needs regular watering, grow and carry out sample analysis after 140 days.
The red three-coloured amaranth of results is placed in to 105 ℃ of baking ovens 30min that completes, then at 70 ℃, dry to constant weight (drying about 48h), weigh its dry weight in the balance.Then the plant sample of drying is ground, accurately take 0.2g processing sample and be placed in triangular flask, add 10ml mixed acid (nitric acid: perchloric acid volume ratio 3:1), add a cover and spend the night.Sample liquid is poured in triumphant formula flask, on electric furnace, clears up, until Mao Baiyan, digestive juice is water white transparency.With 0.5mol/L nitric acid constant volume to 100ml.Adopt flame atomic absorption spectrometry plant sample to be carried out to the mensuration of cadmium content.
Table 6 auxin IAA absorbs in red three-coloured amaranth body cadmium and the impact distributing
Result of the test shows: as can be known from Table 6, under cadmium is processed, the auxin IAA that sprays variable concentrations has increased the content of cadmium at red three-coloured amaranth root and overground part, in the time spraying the IAA of 100mg/L, red three-coloured amaranth root and overground part cadmium content are all maximum, have increased by 30%, 20.2% respectively than contrast.
Table 7 gibberellin GA absorbs in red three-coloured amaranth body cadmium and the impact distributing
Result of the test shows: as can be known from Table 7, under cadmium is processed, the gibberellin GA that sprays variable concentrations can increase the content of cadmium at red three-coloured amaranth root and overground part.In the time spraying the GA of 500mg/L, red three-coloured amaranth root and overground part cadmium content are all maximum, have increased by 74.6%, 26.8% respectively than contrast.
Table 8 salicylic acid SA absorbs in red three-coloured amaranth body cadmium and the impact distributing
Result of the test shows: as can be known from Table 8, under cadmium is processed, the salicylic acid SA that sprays variable concentrations can increase the content of cadmium at red three-coloured amaranth root and overground part (10mg/L exception).In the time spraying the SA of 100mg/L, red three-coloured amaranth root and overground part cadmium content are all maximum, have increased by 62.9%, 33.2% respectively than contrast.
In sum, under cadmium is processed, the plant growth regulator that sprays variable concentrations increase in various degree the root of red three-coloured amaranth and the content of overground part cadmium, and in the time that the concentration for the treatment of of GA is 500mg/L, the root cadmium content of red three-coloured amaranth is the highest, in the time that SA concentration is 100mg/L, the overground part portion cadmium content of red three-coloured amaranth is the highest.
Embodiment 3:
Red three-coloured amaranth cadmium individual plant accumulation characteristic under plant growth regulator treatment
This test is carried out in Xinan Science and Technology Univ.'s life science and engineering college's east eight laboratory buildings, the red three-coloured amaranth seed of participating in the experiment is purchased from Mianyang Yun Chuan seeds company, and control soil is yellow soil, and soil is put in after the natural air drying of lucifuge ventilation, impurities removing, smash to pieces and sieve after for subsequent use.Sowed red three-coloured amaranth on March 19, in the time that seedling grows to 1 month, be colonizated in plastic tub (the diameter 30cm that 6.0kg soil (physicochemical property of soil is in table 2) is housed, high 23cm), wherein Cd is evenly mixed in soil take concentration as 20mg/kg aqueous solution form, continue to cultivate 1 month, May 21 was evenly sprayed on red three-coloured amaranth blade face three plant growth regulators of variable concentrations (auxin, gibberellin, salicylic acid) with the form of the aqueous solution, according to growth needs regular watering, grow and carry out sample analysis after 140 days.
The red three-coloured amaranth of results is placed in to 105 ℃ of baking ovens 30min that completes, then at 70 ℃, dry to constant weight (drying about 48h), weigh its dry weight in the balance.Then the plant sample of drying is ground, accurately take 0.2g processing sample and be placed in triangular flask, add 10ml mixed acid (nitric acid: perchloric acid volume ratio 3:1), add a cover and spend the night.Sample liquid is poured in triumphant formula flask, on electric furnace, clears up, until Mao Baiyan, digestive juice is water white transparency.With 0.5mol/L nitric acid constant volume to 100ml.Adopt flame atomic absorption spectrometry plant sample to be carried out to the mensuration of cadmium content.
Result of the test shows: as can be known from Fig. 1, under 20mg/kg cadmium is processed, the auxin IAA that sprays variable concentrations can promote the accumulation of red three-coloured amaranth to cadmium.In the time using the IAA of 100mg/L and process red three-coloured amaranth, the accumulation maximum of red three-coloured amaranth to cadmium, is 1233.57 μ g/ strains, is 3.12 times of contrast.
Result of the test shows: as can be known from Fig. 2, under 20mg/kg cadmium is processed, the gibberellin GA that sprays variable concentrations can promote the accumulation of red three-coloured amaranth to cadmium.In the time using the GA of 500mg/L and process red three-coloured amaranth, the accumulation maximum of red three-coloured amaranth to cadmium, is 1883.25 μ g/ strains, is 4.76 times of contrast.
Result of the test shows: as can be known from Fig. 3, under 20mg/kg cadmium is processed, the salicylic acid SA that sprays variable concentrations can promote the accumulation of red three-coloured amaranth to cadmium.In the time using the SA of 100mg/L and process red three-coloured amaranth, the accumulation maximum of red three-coloured amaranth to cadmium, is 1578.33 μ g/ strains, is 3.99 times of contrast.
In sum, be under 20mg/kg cadmium is processed in concentration, the plant growth regulator that sprays variable concentrations increase in various degree the amount of red three-coloured amaranth individual plant accumulation cadmium, and when the concentration for the treatment of of GA is 500mg/L, red three-coloured amaranth individual plant accumulates the amount maximum of cadmium.
Embodiment 4:
Red three-coloured amaranth is transported and the characteristic of cadmium plant growth regulator (or claim plant hormone) effect is lower
This test is carried out in Xinan Science and Technology Univ.'s life science and engineering college's east eight laboratory buildings, the red three-coloured amaranth seed of participating in the experiment is purchased from Mianyang Yun Chuan seeds company, and control soil is yellow soil, and soil is put in after the natural air drying of lucifuge ventilation, impurities removing, smash to pieces and sieve after for subsequent use.Sowed red three-coloured amaranth on March 19, in the time that seedling grows to 1 month, be colonizated in plastic tub (the diameter 30cm that 6.0kg soil (physicochemical property of soil is in table 2) is housed, high 23cm), wherein Cd is evenly mixed in soil take concentration as 20mg/kg aqueous solution form, continues to cultivate 1 month, and May 21 was evenly sprayed on red three-coloured amaranth blade face three plant growth regulators of variable concentrations with the form of the aqueous solution, according to growth needs regular watering, grow and carry out sample analysis after 140 days.
The red three-coloured amaranth of results is placed in to 105 ℃ of baking ovens 30min that completes, then at 70 ℃, dry to constant weight (drying about 48h), weigh its dry weight in the balance.Then the plant sample of drying is ground, accurately take 0.2g processing sample and be placed in triangular flask, add 10ml mixed acid (nitric acid: perchloric acid volume ratio 3:1), add a cover and spend the night.Sample liquid is poured in triumphant formula flask, on electric furnace, clears up, until Mao Baiyan, digestive juice is water white transparency.With 0.5mol/L nitric acid constant volume to 100ml.Adopt flame atomic absorption spectrometry plant sample to be carried out to the mensuration of cadmium content.
Transport the calculating of coefficient and concentration coefficient according to the Cd content of Different Organs.Transhipment coefficient is overground part constituent content and the ratio of underground part constituent content, be used for evaluating plant by certain element the transportation from underground part to overground part and accumulation ability.The plant that transhipment coefficient is large is large to the uptake of overground part organ metastasis from root system.The transhipment coefficient of general super enriching plant requires more than 1.Its computing formula is: content of beary metal/plant root content of beary metal in transhipment coefficient=plant shoot organ.
The ratio of respective element content in the constituent content that concentration coefficient refers to a certain position of plant and soil, it is reflecting soil to a certain extent--the complexity of migration of element in botanical system, it reflected plant by element absorption, transfer to the ability in body.Concentration coefficient shows that more greatly the ability of plants enriched element is stronger.The concentration coefficient of general super enriching plant is more than 1.Its computing formula is: content of beary metal/heavy metal in soil content in concentration coefficient=plant shoot organ.
Table 9 auxin IAA processes lower red three-coloured amaranth Cd transhipment coefficient and concentration coefficient
Result of the test shows: as can be seen from Table 9, under the IAA of 10mg/L processes, red three-coloured amaranth is the highest to the transhipment coefficient of cadmium, has increased by 3.1% than contrast, and in the time that IAA concentration is 100mg/L, red three-coloured amaranth is the highest to the concentration coefficient of cadmium, has increased 20.2%. than contrast
Table 10 gibberellin GA processes lower red three-coloured amaranth Cd transhipment coefficient and concentration coefficient
Result of the test shows: as can be seen from Table 10, under the GA of various concentration processes, red three-coloured amaranth is to the concentration coefficient of cadmium all higher than contrast, and when wherein GA concentration is 500mg/L, red three-coloured amaranth is the highest to the concentration coefficient of cadmium, has increased 26.8%. than contrast
Table 11 salicylic acid SA processes lower red three-coloured amaranth Cd transhipment coefficient and concentration coefficient
Result of the test shows: as can be seen from Table 11, in the time that SA concentration is 100mg/L, red three-coloured amaranth is the highest to the concentration coefficient of cadmium, has increased 33.2%. than contrast
In sum, the gibberellin processing of 100mg/L salicylic acid or auxin, 500mg/L has significantly improved the enrichment power of red three-coloured amaranth overground part.
Embodiment 4:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, be: containing planting red three-coloured amaranth in the soil of cadmium contaminants, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth harvesting or entirety are removed from contaminated soil.
Embodiment 5:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, be: on the basis of embodiment 4, after red plant three-coloured amaranth entirety is removed from contaminated soil, plant again the red three-coloured amaranth of next group, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth entirety is removed from contaminated soil, repeat said process, until the cadmium content in soil reaches environmental safety standard.
Embodiment 6:
Utilize plant growth regulator to promote a method for red three-coloured amaranth cadmium, described plant growth regulator is selected auxin (IAA); Plant growth regulator auxin (IAA) is mixed with the aqueous solution of the auxin that concentration is 100mg/L (IAA), and sprays after planting growing for 1～March on the blade face of red three-coloured amaranth of (can be wherein people one day); The other the same as in Example 4 or 5, omits.
Embodiment 7:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected gibberellin (GA), gibberellin of plant growth regulator (GA) is mixed with the aqueous solution of the gibberellin that concentration is 500mg/L (GA), and sprays after planting growing for 1～March on the blade face of red three-coloured amaranth of (can be wherein people one day); The other the same as in Example 4 or 5, omits.
Embodiment 8:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected salicylic acid (SA), plant growth regulator salicylic acid (SA) is mixed with the aqueous solution of the salicylic acid that concentration is 100mg/L (SA), and sprays after planting growing for 1～March on the blade face of red three-coloured amaranth of (can be wherein people one day); The other the same as in Example 4 or 5, omits.
Embodiment 9:
Utilize plant growth regulator to promote a method for red three-coloured amaranth cadmium, described plant growth regulator is selected auxin (IAA); Plant growth regulator auxin (IAA) is mixed with the aqueous solution of the auxin that concentration is 100mg/L (IAA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 1 month; The other the same as in Example 4 or 5, omits.
Embodiment 10:
Utilize plant growth regulator to promote a method for red three-coloured amaranth cadmium, described plant growth regulator is selected auxin (IAA); Plant growth regulator auxin (IAA) is mixed with the aqueous solution of the auxin that concentration is 100mg/L (IAA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 2 months; The other the same as in Example 4 or 5, omits.
Embodiment 11:
Utilize plant growth regulator to promote a method for red three-coloured amaranth cadmium, described plant growth regulator is selected auxin (IAA); Plant growth regulator auxin (IAA) is mixed with the aqueous solution of the auxin that concentration is 100mg/L (IAA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 3 months; The other the same as in Example 4 or 5, omits.
Embodiment 12:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected gibberellin (GA), gibberellin of plant growth regulator (GA) is mixed with the aqueous solution of the gibberellin that concentration is 500mg/L (GA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 1 month; The other the same as in Example 4 or 5, omits.
Embodiment 13:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected gibberellin (GA), gibberellin of plant growth regulator (GA) is mixed with the aqueous solution of the gibberellin that concentration is 500mg/L (GA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 2 months; The other the same as in Example 4 or 5, omits.
Embodiment 14:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected gibberellin (GA), gibberellin of plant growth regulator (GA) is mixed with the aqueous solution of the gibberellin that concentration is 500mg/L (GA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 3 months; The other the same as in Example 4 or 5, omits.
Embodiment 15:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected salicylic acid (SA), plant growth regulator salicylic acid (SA) is mixed with the aqueous solution of the salicylic acid that concentration is 100mg/L (SA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 1 month; The other the same as in Example 4 or 5, omits.
Embodiment 16:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected salicylic acid (SA), plant growth regulator salicylic acid (SA) is mixed with the aqueous solution of the salicylic acid that concentration is 100mg/L (SA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 2 months; The other the same as in Example 4 or 5, omits.
Embodiment 17:
A kind of method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, described plant growth regulator is selected salicylic acid (SA), plant growth regulator salicylic acid (SA) is mixed with the aqueous solution of the salicylic acid that concentration is 100mg/L (SA), and sprays on the blade face of the red three-coloured amaranth of after planting growing 3 months; The other the same as in Example 4 or 5, omits.
Embodiment 18:
Utilize plant growth regulator to promote a method for red three-coloured amaranth cadmium, also comprise: adopt the method for burning to carry out harmless treatment (after burning, the residual solids that contains cadmium can further adopt prior art modes such as reclaiming metal, landfill to process) harvesting or the overall red three-coloured amaranth of removing from contaminated soil; Arbitrary in the other the same as in Example 4～17, omit.
The time of the sowing of described red three-coloured amaranth can be annual February to October (being spring, summer, autumn all can sow and plant); The method of plantation is sowing, and every 666.7 square metres of application rates are 0.5～0.75 kilogram; The sowing NaClO(clorox that is 0.5% with red three-coloured amaranth seed mass percent concentration) after aqueous solution soaking 15～25min, be used further to sow.Not concrete or incomplete part in red three-coloured amaranth planting patterns, with the plantation of red three-coloured amaranth of being used as vegetables in prior art.
In above-described embodiment: the aqueous solution of described plant growth regulator is amount of application (spraying the amount on the blade face at red three-coloured amaranth) in every 666.7 square metres of soil: described concentration is 100mg/L(mg/litre) the amount of application of the aqueous solution of auxin preferably for 20L(liter); Described concentration is that the amount of application of the aqueous solution of the gibberellin of 500mg/L is 20L(liter preferably); Described concentration is that the amount of application of the salicylic aqueous solution of 100mg/L is 20L(liter preferably); Each amount of application also can be at 15～25L(liter) in/every 666.7 square metres of soil in.
In above-described embodiment: the each raw material adopting is commercially available prod.
In above-described embodiment: in the percentage adopting, do not indicate especially, be quality (weight) percentage or well known to a person skilled in the art percentage; Described quality (weight) part can be all gram or kilogram.
In above-described embodiment: technological parameter (temperature, time, concentration etc.) and each amounts of components numerical value etc. in each step is scope, and any point is all applicable.
The not concrete same prior art of technology contents of narrating in content of the present invention and above-described embodiment.
Can find out from stating embodiment, plant growth regulator (or claiming plant hormone) not only can improve the biomass of red three-coloured amaranth, also the cadmium extracting in soil is had to very strong facilitation, therefore, apply plant hormone and can promote absorption, transhipment and the enrichment of red three-coloured amaranth to cadmium, thereby the repair time that shortens cadmium pollution soil, be significant for the phytoremediation of cadmium pollution soil.
The invention is not restricted to above-described embodiment, all can implement and have described good result described in content of the present invention.
Claims (8)
1. a method of utilizing plant growth regulator to promote red three-coloured amaranth cadmium, it is characterized in that comprising: containing planting red three-coloured amaranth in the soil of cadmium contaminants, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth harvesting or entirety are removed from contaminated soil.
2. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 1, it is characterized in that also comprising: described red plant three-coloured amaranth entirety is removed from contaminated soil after, plant again the red three-coloured amaranth of next group, and spray plant growth regulator, in the time that the red three-coloured amaranth of plant grows to florescence or maturity period, red plant three-coloured amaranth entirety is removed from contaminated soil, repeated said process, until the cadmium content in soil reaches environmental safety standard.
3. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 3, it is characterized in that: described plant growth regulator is auxin, gibberellin or salicylic acid.
4. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 3, it is characterized in that: described plant growth regulator auxin is mixed with the aqueous solution that concentration is the auxin of 100mg/L, and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
5. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 3, it is characterized in that: described gibberellin of plant growth regulator is mixed with the aqueous solution that concentration is the gibberellin of 500mg/L, and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
6. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 3, it is characterized in that: described plant growth regulator salicylic acid is mixed with the salicylic aqueous solution that concentration is 100mg/L, and sprays on the blade face of the red three-coloured amaranth of after planting growing for 1～March.
7. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 1,2,4,5 or 6, it is characterized in that also comprising: adopt the method for burning to carry out harmless treatment harvesting or the overall red three-coloured amaranth of removing from contaminated soil.
8. by utilizing plant growth regulator to promote the method for red three-coloured amaranth cadmium described in claim 3, it is characterized in that also comprising: adopt the method for burning to carry out harmless treatment harvesting or the overall red three-coloured amaranth of removing from contaminated soil.
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| CN104128362A (en) * | 2014-07-14 | 2014-11-05 | 华南农业大学 | Method for restoring cadmium polluted soil by Thalia dealbata |
| CN106001100A (en) * | 2016-05-31 | 2016-10-12 | 四川农业大学 | Method for remarkably enhancing capacity of starwort for restoring orchard cadmium-polluted soil |
| CN107413831A (en) * | 2017-07-05 | 2017-12-01 | 湖南农业大学 | One kind promotes red three-coloured amaranth growth to reduce heavy metal-polluted soil mercury pollution method |
| CN108380667A (en) * | 2018-02-06 | 2018-08-10 | 中国科学院华南植物园 | A method of it improving rehabilitation plant and absorbs Cadmium in Soil efficiency |
| CN108593496A (en) * | 2018-04-25 | 2018-09-28 | 江汉大学 | A kind of measurement method of crops cadmium ion content |
| CN108655159A (en) * | 2018-05-15 | 2018-10-16 | 江苏大学 | A kind of combination biochemical method of enhancing phytoremediation pollution efficiency |
| CN109622601A (en) * | 2018-12-04 | 2019-04-16 | 浙江清华长三角研究院 | A method of utilizing Low Concentration Mercury polluted farmland soil in cotton and Indian mustard crop rotation reparation |
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| CN111760905A (en) * | 2020-07-08 | 2020-10-13 | 安徽师范大学 | Method for repairing cadmium-polluted soil by using plants and phytohormones together |
| CN113372917A (en) * | 2021-06-07 | 2021-09-10 | 沈阳大学 | Method for strengthening phytoremediation of cadmium-polluted soil by using regulator and product thereof |
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| CN104128362A (en) * | 2014-07-14 | 2014-11-05 | 华南农业大学 | Method for restoring cadmium polluted soil by Thalia dealbata |
| CN106001100A (en) * | 2016-05-31 | 2016-10-12 | 四川农业大学 | Method for remarkably enhancing capacity of starwort for restoring orchard cadmium-polluted soil |
| CN107413831A (en) * | 2017-07-05 | 2017-12-01 | 湖南农业大学 | One kind promotes red three-coloured amaranth growth to reduce heavy metal-polluted soil mercury pollution method |
| CN108380667A (en) * | 2018-02-06 | 2018-08-10 | 中国科学院华南植物园 | A method of it improving rehabilitation plant and absorbs Cadmium in Soil efficiency |
| CN108593496A (en) * | 2018-04-25 | 2018-09-28 | 江汉大学 | A kind of measurement method of crops cadmium ion content |
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| CN109622601A (en) * | 2018-12-04 | 2019-04-16 | 浙江清华长三角研究院 | A method of utilizing Low Concentration Mercury polluted farmland soil in cotton and Indian mustard crop rotation reparation |
| CN110064647A (en) * | 2019-06-05 | 2019-07-30 | 湖南双晟科技信息咨询有限公司 | A kind of processing method of cadmium pollution soil |
| CN111438182A (en) * | 2020-04-28 | 2020-07-24 | 贵州大学 | Enhanced remediation method for remediating cadmium-contaminated soil by using hyper-enriched plants |
| CN111760905A (en) * | 2020-07-08 | 2020-10-13 | 安徽师范大学 | Method for repairing cadmium-polluted soil by using plants and phytohormones together |
| CN113372917A (en) * | 2021-06-07 | 2021-09-10 | 沈阳大学 | Method for strengthening phytoremediation of cadmium-polluted soil by using regulator and product thereof |
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