CN103521514A - Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid - Google Patents
Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid Download PDFInfo
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Abstract
The invention relates to a method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid. Ryegrass is planted in the lead contaminated soil, gibberellin (GA3) is added in a foliar spray form, and the ethylenediamine tetraacetic acid (EDTA) is directly added into the soil, therefore, the purpose of strengthening remediation of the lead contaminated soil by the plants is achieved. The GA3 at the concentration of 1mu M or 10mu M can obviously relief toxicity for the plants caused by Pb and/or EDTA, and the effect of strengthening remediation of the EDTA can be significantly improved. However, 100mu M GA3 inhibits the growth of the plants, and significantly reduces the concentration of lead in the plants. The method provided by the invention is used for carrying out in situ remediation, has the advantages of low cost, simple process, environmental friendliness and the like, has a good remediation effect on lead contaminated soil, and is suitable for remediation of lead contaminated soil.
Description
Technical field
The present invention relates to strengthen remediating heavy metal lead-contaminated soil technology, relate to a kind of method of gibberellin, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, specifically a kind of gibberellin (GA that utilizes
3) and the method for the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid (ETDA).
Background technology
That phytoremediation technology has is simple to operate, economy, environmental friendliness and be easy to as advantages such as people are accepted, has become the study hotspot that heavy metal pollution of soil is repaired field.Because the Phyto-availability of Lead In Soil is low, and heavy metal super-enriched plant poor growth, biological little, and in the large plant of biomass, heavy metal concentration is low, and this has seriously limited the application of phytoremediation technology in actual contaminated soil is repaired.Therefore, many scholars have proposed to use chelating agent to improve the accumulation ability of plant to heavy metal, and wherein EDTA is the most frequently used chelating agent.EDTA can improve the plant availability of heavy metal in rhizosphere soil, promote the transportation of heavy metal from root system to overground part, thereby significantly improve plant to the accumulation ability of heavy metal (document 1:L ó pez M.L., Peralta-Videa J.R., Benitez T., et al.Enhancement of lead uptake by alfalfa (Medicago sativa) using EDTA and a plant growth promoter[J] .Chemosphere, 2005, 61:595-598. document 2: Wang Hongxin, Guo Shaoyi, Hu Feng, Deng. the impact [J] of chelating agent on castor-oil plant growth of seedling and the accumulation of plumbous zinc in Pb-Zn tailings improvement matrix. soil journal, 2012, 49 (3): 491-498.).Yet the growth of the chelate meeting severe inhibition plant that EDTA and heavy metal form, reduces biomass, thereby affects phytoremediation effect.
Gibberellin is the plant hormone that plant kingdom extensively exists, it can accelerate cell division, mature cell longitudinal tensile strain, internodal cell elongation, prolongation and the germination (document 3: Wang Yanbo of the growth of regulating plant and promotion stem, aquatic foods are opened plum, Zhang Yonghua, the application study progress [J] of Liu Hui English .2007. gibberellin. northern gardening, 6:74-75.).In gibberellin, that the strongest, the most study of physiologically active is GA
3(Gibberellic Acid), it can promote axis, leaf growth significantly.But there is not yet so far relevant gibberellin (GA
3) separately or with the report of ethylenediamine tetra-acetic acid (ETDA) jointly enhancing plants remediating lead-contaminated soil.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of method of gibberellin, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid is provided.
For achieving the above object, technology contents of the present invention is:
In lead-contaminated soil to be repaired, plant rye grass, after plant germination the 7th day, foliar spray 5mLGA
3solution, in soil, add the EDTA solution that 5mL concentration is 2.5mmol/kg simultaneously, carrying out altogether 4 times processes, be 7 days each interval time of processing, plant integral body was removed in 42 days after plant germination, then plant second batch plant, repeat aforesaid operations, until the lead content in soil reaches environmental safety standard, thereby reach the object of remediating lead-contaminated soil.
Wherein, GA
3concentration be 1 μ M, 10 μ M or 100 μ M.In growing process, regularly water, remain that soil moisture content is 75 ﹪ of maxmun field capacity.
The invention has the beneficial effects as follows:
1. the present invention adds 1 μ M or 10 μ M GA
3can Promoting plant growth, show as the increase of biomass.
2. the present invention adds EDTA and is conducive to improve plumbous biological effectiveness in soil, promotes plant to plumbous extraction and enrichment; Meanwhile, concentration is 1 μ M or 10 μ M GA
3interpolation improve the biomass of plant, thereby obviously improve the repairing effect of plant to lead-contaminated soil, wherein with 1 μ M GA
3most effective with the compound action of EDTA.
3. if use separately 100 μ M GA
3not only suppress plant growth, and significantly reduce concentration plumbous in plant, but with 100 μ M GA
3still better to the repairing effect of lead-contaminated soil with plant under the compound action of EDTA.
Accompanying drawing explanation
The detection figure of the different disposal that Fig. 1 provides for the embodiment of the present invention 1 to rye grass biomass;
The detection figure of the different disposal that Fig. 2 provides for the embodiment of the present invention 2 to rye grass biomass;
The detection figure of the different disposal that Fig. 3 provides for the embodiment of the present invention 3 to rye grass biomass.
The specific embodiment
Below in conjunction with accompanying drawing, the present invention is further analyzed.
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 250mg/kg.Research is found, compare with disposable interpolation, when divided dose adds EDTA, plant is to the extraction of Lead In Soil and larger (the document 4:Hadi of transhipment amount, F., Bano, A., Fuller, M.P.2010.The improved phytoextraction of lead (Pb) and the growth of maize (Zea mays L.): the role of plant growth regulators (GA
3and IAA) and EDTA alone and in combinations[J] .Chemosphere80,457-462.).And, when EDTA concentration is 2.5mmol/kg, buckwheat and sunflower are the strongest to plumbous absorption and turn-over capacity, therefore EDTA concentration is made as in this experiment to 2.5mmol/kg(document 5:Chen, Y.H., Li, X.D., et al., 2004.Leaching and uptake of heavy metals by ten different species of plants during an EDTA-assisted phytoextraction process[J] .Chemosphere57,187-196.).After crossing 5mm sieve for examination soil, fill basin, every basin 18.5cm (diameter) * 21cm (height) fills native 2kg (dry weight), basin underlay pallet.In confession examination soil, Pb is with Pb (NO
3)
2the form of the aqueous solution adds, and fully mixes, and recording Lead In Soil concentration is 250mg/kg, then adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), add 2.5mmol/kg EDTA (T1), foliar spray 1 μ M GA
3(T2), foliar spray 10 μ M GA
3(T3), foliar spray 100 μ M GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μ M GA
3(T5), 2.5mmol/kg EDTA+ foliar spray 10 μ M GA
3(T6), 2.5mmol/kgEDTA+ foliar spray 100 μ M GA
3(T7).Each experimental group is all established 3 repetitions.Experimental group C in contrast.Ryegrass seed carries out GA after germinateing 7 days
3with the interpolation alone or in combination of EDTA, carry out week about GA
3foliar spray or/and EDTA directly add in soil.GA
3with the each addition of EDTA be all 5mL, add altogether 4 times.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, with running water, repeatedly rinses plant, finally with blotting paper, surperficial water is blotted.At 75 ℃, dry to weight, after weighing dry weight, pulverize standby.
Rye grass biomass changes as shown in Figure 1.As seen from Figure 1, except EDTA processes separately, other process the growth that all promotes rye grass, show as the increase of biomass.Compared with the control, under EDTA processes separately, rye grass Aboveground Biomass of Young 19.4 ﹪ that declined.Compared with the control, experimental group T2, T3 make the biomass of rye grass increase by 13.3 ﹪～45.1 ﹪, wherein at 10 μ M GA
3during processing, it is maximum that phytomass reaches.On the contrary, foliar spray 100 μ M GA
3significantly suppressed the growth of plant.Add EDTA and concentration is the GA of 1 μ M or 10 μ M simultaneously
3, the biomass of plant is along with GA
3the increase of concentration and increasing, compared with the control, the biomass of rye grass has increased by 1.5 ﹪～25.2 ﹪, but with single concentration GA
3processing is compared, and under Combined Processing, rye grass biomass declines.But at EDTA+100 μ M GA
3under processing, phytomass has reduced by 28.7 ﹪ than contrast.Above result of study shows, concentration is the GA of 1 μ M or 10 μ M
3effectively alleviated the inhibition of EDTA to Ryegrass Growth in soil, but 100 μ M GA
3growth table to rye grass reveals inhibitory action.
Pb content in rye cursive script under table 1 different disposal
Table 1 can find out, different disposal all promotes rye grass to the extraction of Pb and enrichment.Concentration is the GA of 1 μ M or 10 μ M<sub TranNum="114">3</sub>significantly promoted rye grass root and the absorption of aerial part to Pb, compared with the control, root and aerial part Pb content have increased respectively 11.4 ﹪～26.1 ﹪ and 48.7 ﹪～99.1 ﹪, wherein with 1 μ M GA<sub TranNum="115">3</sub>act on the most remarkable.10 μ M GA<sub TranNum="116">3</sub>make root and aerial part Pb content reduce respectively 18.7 ﹪ and 32.1 ﹪.EDTA and GA<sub TranNum="117">3</sub>rye grass Pb is extracted Combined Processing and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration have increased respectively 1.4～2.1 and 5.8～8.1 times compared with the control.Wherein, under T5 experimental group, it is maximum that plant each several part Pb reaches, and root and aerial part reach respectively 802.1mg/kg and 500.6mg/kg, and its aerial part Pb content is significantly higher than other experimental group (P < 0.05).
Table 2 different disposal absorbs the impact of Pb on plant
From table 2, except T4 experimental group, the rye grass of other experimental group all significantly improves the enrichment of Pb and transhipment coefficient.Concentration is the GA of 1 μ M or 10 μ M
3under processing, the enrichment of Pb in rye cursive script and transhipment coefficient rise with the increase of concentration; But 100 μ M GA
3make enrichment and the transhipment coefficient of rye cursive script lead reduce respectively 31.6 ﹪ and 18.2 ﹪.Compared with the control, GA
3combine with EDTA and add promotion plant to the extraction of Pb and enrichment, plant has increased respectively 5.8～8.5 and 1.8 times to the enrichment of Pb and transhipment coefficient, wherein the most remarkable with the processing effect of T5 experimental group.
The impact of table 3 different disposal on plant Pb extracted amount and extraction efficiency
Rye grass is 0.06 ﹪～1.1 ﹪ (table 3) to the extraction efficiency of Pb.Except T4 experimental group, GA
3separately or all significantly promoted the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3.In different disposal, under T5 experimental group is processed, rye grass reaches maximum to the extracted amount of Pb and extraction efficiency, and this processing is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μ M or 10 μ M
3significantly improve the biomass of plant with EDTA synergy, promoted plant to the absorption of Pb and accumulation.Wherein, T5 experimental group is best suited for strengthening rye grass remediating lead-contaminated soil.But 100 μ M GA
3independent role all plays inhibitory action to Ryegrass Growth and plumbous enrichment.
Embodiment 2
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 500mg/kg.After crossing 5mm sieve for examination soil, fill basin, every basin 18.5cm (diameter) * 21cm (height) fills native 2kg (dry weight), basin underlay pallet.For trying P in soil b with Pb (NO
3)
2the form of the aqueous solution adds soil, and fully mixes, and recording Lead In Soil concentration is 500mg/kg, adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), add 2.5mmol/kg EDTA (T1), foliar spray 1 μ M GA
3(T2), foliar spray 10 μ M GA
3(T3), foliar spray 100 μ M GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μ M GA
3(T5), 2.5mmol/kg EDTA+ foliar spray 10 μ M GA
3(T6), 2.5mmol/kg EDTA+ foliar spray 100 μ M GA
3(T7).Each experimental group is all established 3 repetitions.Experimental group C in contrast.Ryegrass seed carries out GA after germinateing 7 days
3with the interpolation alone or in combination of EDTA, carry out week about GA
3foliar spray or/and EDTA directly add in soil.GA
3with the each addition of EDTA be all 5mL, add altogether 4 times.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, with running water, repeatedly rinses plant, finally with blotting paper, surperficial water is blotted.At 75 ℃, dry to weight, after weighing dry weight, pulverize standby.
Rye grass biomass changes as shown in Figure 2.As seen from Figure 2, except EDTA processes, different disposal all promotes the growth of rye grass, shows as the increase of biomass.Compared with the control, under EDTA processes separately, rye grass Aboveground Biomass of Young 16.3 ﹪ that declined.Foliar spray concentration is the GA of 1 μ M or 10 μ M
3make the biomass of rye grass increase by 18.7 ﹪～52.5 ﹪, wherein, when T2 experimental group is processed, it is maximum that phytomass reaches.But 100 μ M GA
3significantly suppressed the growth of plant.Add EDTA and concentration is the GA of 1 μ M or 10 μ M simultaneously
3, make the biomass of rye grass increase by 9.9 ﹪～31.2 ﹪, but with single concentration GA
3processing is compared, and under Combined Processing, rye grass biomass declines.On the contrary, 100 μ MGA
3+ EDTA makes rye grass biomass reduce by 24.2 ﹪.Above result of study shows, concentration is the GA of 1 μ M or 10 μ M
3effectively alleviated the inhibition of EDTA to Ryegrass Growth in soil, but 100 μ M GA
3growth table to rye grass reveals inhibitory action.
Pb content in rye cursive script under table 4 different disposal
Table 4 can find out, different disposal all promotes rye grass to the extraction of Pb and enrichment.Concentration is the GA of 1 μ M or 10 μ M<sub TranNum="166">3</sub>significantly promoted rye grass root and the absorption of aerial part to Pb, compared with the control, root and aerial part Pb content have increased respectively 17.6 ﹪～29.2 ﹪ and 41.4 ﹪～116.3 ﹪, wherein with 1 μ M GA<sub TranNum="167">3</sub>act on the most remarkable.On the contrary, 100 μ M GA<sub TranNum="168">3</sub>make rye grass root and aerial part lead concentration reduce respectively 23.3 ﹪ and 25.7 ﹪.EDTA and GA<sub TranNum="169">3</sub>rye grass Pb is extracted Combined Processing and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration have increased respectively 1.2～2.0 and 5.0～7.2 times compared with the control.Wherein, under T5 experimental group is processed, it is maximum that plant each several part Pb reaches, and root and aerial part reach respectively 1654.5mg/kg and 1250.6mg/kg, and its aerial part Pb content is significantly higher than other and processes (P < 0.05).
Table 5 different disposal absorbs the impact of Pb on plant
From table 5, except 100 μ M GA
3outside processed group, under different disposal, rye grass all significantly improves the enrichment of Pb and transhipment coefficient separately.Compared with the control, foliar spray concentration is the GA of 1 μ M or 10 μ M
3make the enrichment of Pb in rye cursive script and transhipment coefficient improve 0.4～1.2 and 0.2～0.6 times.Compared with the control, GA
3combine interpolation with EDTA and promote plant to the extraction of Pb and enrichment, plant has increased respectively 5.1～7.2 and 1.7 times to the enrichment of Pb and transhipment coefficient, and under wherein processing with T5, effect is the most remarkable.
The impact of table 6 different disposal on plant Pb extracted amount and extraction efficiency
Rye grass is 0.08 ﹪～1.3 ﹪ (table 6) to the extraction efficiency of Pb.Concentration is the GA of 1 μ M or 10 μ M
3separately or all significantly promoted the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3, but 100 μ M GA
3independent role makes the extracted amount of rye grass lead and extraction efficiency reduce respectively 34.6 ﹪ and 33.3 ﹪.Under T5 processes, rye grass reaches maximum to the extracted amount of Pb and extraction efficiency, and this processing is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μ M or 10 μ M
3significantly improve the biomass of plant with EDTA synergy, promoted plant to the absorption of Pb and accumulation.Wherein, T5 experimental group is processed and is best suited for strengthening rye grass remediating lead-contaminated soil.But 100 μ M GA
3independent role has suppressed the growth of plant and it is to plumbous extraction and enrichment.
Embodiment 3
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 750mg/kg.For examination soil, cross dress basin after 5mm sieve, every basin 18.5cm(diameter) * 21cm(is high) fill native 2kg (dry weight), basin underlay pallet.In confession examination soil, Pb is with Pb (NO
3)
2the form of the aqueous solution adds soil, and fully mixes, and the lead concentration that records soil is 750mg/kg, then adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), add 2.5mmol/kg EDTA (T1), foliar spray 1 μ M GA
3(T2), foliar spray 10 μ M GA
3(T3), foliar spray 100 μ M GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μ M GA
3(T5), 2.5mmol/kgEDTA+ foliar spray 10 μ M GA
3(T6), 2.5mmol/kg EDTA+ foliar spray 100 μ M GA
3(T7).Each experimental group is all established 3 repetitions.Experimental group C in contrast.Ryegrass seed carries out GA after germinateing 7 days
3with the interpolation alone or in combination of EDTA, carry out week about GA
3foliar spray or/and EDTA directly add in soil.GA
3with the each addition of EDTA be all 5mL, add altogether 4 times.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, with running water, repeatedly rinses plant, finally with blotting paper, surperficial water is blotted.At 75 ℃, dry to weight, after weighing dry weight, pulverize standby.
Rye grass biomass changes as shown in Figure 3.As seen from Figure 3, except EDTA processes, different disposal all promotes the growth of rye grass, shows as the increase of biomass.Compared with the control, under EDTA processes separately, rye grass Aboveground Biomass of Young 11.7 ﹪ that declined.Compared with the control, concentration is the GA of 1 μ M or 10 μ M
3process and make the biomass of rye grass increase by 26.1 ﹪～62.5 ﹪ separately, wherein, when T2 experimental group is processed, it is maximum that phytomass reaches, but 100 μ M GA
3processing reduces rye grass biomass.Add EDTA and concentration is the GA of 1 μ M or 10 μ M simultaneously
3, the biomass of plant is along with GA
3the increase of concentration and increasing.Compared with the control, the biomass of rye grass has increased by 14.1 ﹪～40.3 ﹪, but with single concentration GA
3processing is compared, and under Combined Processing, rye grass biomass declines.Above result of study shows, concentration is the GA of 1 μ M or 10 μ M
3effectively alleviated the inhibition of EDTA to Ryegrass Growth in soil.On the contrary, 100 μ M GA
3separately or all significantly suppressed the growth of rye grass with EDTA synergy.
Pb content in rye cursive script under table 7 different disposal
Table 7 can find out, different disposal all promotes rye grass to the extraction of Pb and enrichment.Concentration is the GA of 1 μ M or 10 μ M<sub TranNum="215">3</sub>significantly promoted rye grass root and the absorption of aerial part to Pb, compared with the control, root and aerial part Pb content have increased respectively 0.5 ﹪～8.5 ﹪ and 15.0 ﹪～46.1 ﹪, wherein with 1 μ M GA<sub TranNum="216">3</sub>act on the most remarkable.On the contrary, 100 μ M GA<sub TranNum="217">3</sub>process and make rye grass root and aerial part lead concentration reduce respectively 23.4 ﹪ and 17.1 ﹪ separately.EDTA and GA<sub TranNum="218">3</sub>rye grass Pb is extracted Combined Processing and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration have increased respectively 1.0～1.6 and 3.4～5.6 times compared with the control.Wherein, under T5 processes, it is maximum that plant each several part Pb reaches, and root and aerial part reach respectively 2430.5mg/kg and 2452.4mg/kg, and its aerial part Pb content is significantly higher than other and processes (P < 0.05).
Table 8 different disposal absorbs the impact of Pb on plant
From table 8, except 100 μ M GA
3outside processed group, under different disposal, rye grass all significantly improves the enrichment of Pb and transhipment coefficient separately.Compared with the control, GA
3combine interpolation with EDTA and promote plant to the extraction of Pb and enrichment, plant has increased respectively 3.5～5.6 and 1.3～1.5 times to the enrichment of Pb and transhipment coefficient, and under wherein processing with T5, effect is the most remarkable.
The impact of table 9 different disposal on plant Pb extracted amount and extraction efficiency
Rye grass is 0.13 ﹪～1.5 ﹪ (table 9) to the extraction efficiency of Pb.Except 100 μ M GA
3outside independent processed group, GA
3separately or all significantly promoted the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3.T5 experimental group is processed lower rye grass the extracted amount of Pb and extraction efficiency is reached to maximum, and this processing is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μ M or 10 μ M
3significantly improve the biomass of plant with EDTA synergy, promoted plant to the absorption of Pb and accumulation.Wherein, T5 experimental group is processed and is best suited for strengthening rye grass remediating lead-contaminated soil.On the contrary, 100 μ M GA
3independent role has suppressed the growth of plant and it is to plumbous extraction and enrichment.
Claims (4)
1. the method for gibberellin, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, is characterized in that the method specifically plants rye grass in lead-contaminated soil to be repaired, after plant germination the 7th day, and foliar spray GA
3solution, in soil, add EDTA solution simultaneously, carrying out altogether 4 times processes, be 7 days each interval time of processing, plant integral body was removed in 42 days after plant germination, then plant second batch plant, repeat aforesaid operations, until the lead content in soil reaches environmental safety standard, thereby reach the object of remediating lead-contaminated soil;
In growing process, regularly water, remain that soil moisture content is 75 ﹪ of maxmun field capacity.
2. the method for gibberellin as claimed in claim 1, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, is characterized in that GA
3the concentration of solution is 1 μ M, 10 μ M or 100 μ M.
3. the method for gibberellin as claimed in claim 1, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, the concentration that it is characterized in that EDTA solution is 2.5mmol/kg.
4. the method for gibberellin as claimed in claim 1, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, is characterized in that the addition of each processing procedure GA3 solution and EDTA solution is 5mL.
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| CN103962367A (en) * | 2014-04-15 | 2014-08-06 | 西南科技大学 | Method for promoting enrichment of cadmium by red spinach through composition of plant hormone and chelating agent |
| CN106238445A (en) * | 2016-07-08 | 2016-12-21 | 常州大学 | A kind of method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil |
| CN108655159A (en) * | 2018-05-15 | 2018-10-16 | 江苏大学 | A kind of combination biochemical method of enhancing phytoremediation pollution efficiency |
| CN109317512A (en) * | 2018-09-29 | 2019-02-12 | 常州大学 | A kind of method that EDTA strengthens asparagus remediating lead-contaminated soil |
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| CN106238445A (en) * | 2016-07-08 | 2016-12-21 | 常州大学 | A kind of method utilizing EDTA and brassin lactone combined induction rye grass remediating lead-contaminated soil |
| CN108655159A (en) * | 2018-05-15 | 2018-10-16 | 江苏大学 | A kind of combination biochemical method of enhancing phytoremediation pollution efficiency |
| CN109317512A (en) * | 2018-09-29 | 2019-02-12 | 常州大学 | A kind of method that EDTA strengthens asparagus remediating lead-contaminated soil |
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