CN103521514B - Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid - Google Patents
Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid Download PDFInfo
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- CN103521514B CN103521514B CN201310496224.1A CN201310496224A CN103521514B CN 103521514 B CN103521514 B CN 103521514B CN 201310496224 A CN201310496224 A CN 201310496224A CN 103521514 B CN103521514 B CN 103521514B
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Abstract
The invention relates to a method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid. Ryegrass is planted in the lead contaminated soil, gibberellin (GA3) is added in a foliar spray form, and the ethylenediamine tetraacetic acid (EDTA) is directly added into the soil, therefore, the purpose of strengthening remediation of the lead contaminated soil by the plants is achieved. The GA3 at the concentration of 1mu M or 10mu M can obviously relief toxicity for the plants caused by Pb and/or EDTA, and the effect of strengthening remediation of the EDTA can be significantly improved. However, 100mu M GA3 inhibits the growth of the plants, and significantly reduces the concentration of lead in the plants. The method provided by the invention is used for carrying out in situ remediation, has the advantages of low cost, simple process, environmental friendliness and the like, has a good remediation effect on lead contaminated soil, and is suitable for remediation of lead contaminated soil.
Description
Technical field
The present invention relates to strengthening remediating heavy metal lead-contaminated soil technology, relate to a kind of method of gibberellin, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, specifically one utilizes gibberellin (GA
3) and the method for ethylenediamine tetra-acetic acid (ETDA) combined reinforced remediating lead-contaminated soil.
Background technology
Phytoremediation technology has simple to operate, economy, environmental friendliness and is easy to as advantages such as people are accepted, has become the study hotspot that heavy metal pollution of soil repairs field.Because the Phyto-availability of Lead In Soil is low, and heavy metal super-enriched plant poor growth, biological little, and in the large plant of biomass, heavy metal concentration is low, this severely limits the application of phytoremediation technology in actual contaminated soil remediation.Therefore, many scholars propose the accumulation ability using chelating agent to improve plant heavy metal, and wherein EDTA is the most frequently used chelating agent.EDTA can improve the plant availability of heavy metal in rhizosphere soil, promote that heavy metal is from root system to the transport of overground part, thus significantly improve accumulation ability (the document 1:L ó pez M.L. of plant heavy metal, Peralta-Videa J.R., Benitez T., et al.Enhancement of lead uptake by alfalfa (Medicago sativa) usingEDTA and a plant growth promoter [J] .Chemosphere, 2005, 61:595-598. document 2: Wang Hongxin, Guo Shaoyi, Hu Feng, Deng. chelating agent is on the impact [J] of castor-oil plant growth of seedling and the accumulation of plumbous zinc in Pb-Zn tailings improvement matrix. soil journal, 2012, 49 (3): 491-498.).But the chelate that EDTA and heavy metal are formed seriously can suppress the growth of plant, reduces biomass, thus affects effect of plant restoration.
Gibberellin is the plant hormone that plant kingdom extensively exists, it can accelerate cell division, mature cell longitudinal tensile strain, internodal cell elongation, the growth of regulating plant and promote prolongation and the germination (document 3: Wang Yanbo of stem, freshly open plum, Zhang Yonghua, application study progress [J] of Liu Hui English .2007. gibberellin. northern gardening, 6:74-75.).In gibberellin, that the strongest, the most study of physiologically active is GA
3(Gibberellic Acid), it can promote that axis, leaf grow significantly.But there is not yet relevant gibberellin (GA so far
3) separately or with the report of ethylenediamine tetra-acetic acid (ETDA) jointly enhancing plants remediating lead-contaminated soil.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, the method for the combined reinforced remediating lead-contaminated soil of a kind of gibberellin, ethylenediamine tetra-acetic acid is provided.
For achieving the above object, technology contents of the present invention is:
Rye grass is planted, after plant germination the 7th day, foliar spray 5mLGA in lead-contaminated soil to be repaired
3solution, in soil, add the EDTA solution that 5mL concentration is 2.5mmol/kg simultaneously, carry out 4 process altogether, the interval time of each process is 7 days, within 42 days after plant germination, plant entirety is removed, then plants second batch plant, repeat aforesaid operations, until the lead content in soil reaches environmental safety standard, thus reach the object of remediating lead-contaminated soil.
Wherein, GA
3concentration be 1 μM, 10 μMs or 100 μMs.Regularly water in growing process, remain that soil moisture content is 75 ﹪ of maxmun field capacity.
The invention has the beneficial effects as follows:
1. the present invention adds 1 μM or 10 μMs of GA
3can Promoting plant growth, show as the increase of biomass.
2. the present invention adds EDTA and is conducive to improving plumbous biological effectiveness in soil, promotes that plant is to the extraction of lead and enrichment; Meanwhile, concentration is 1 μM or 10 μMs of GA
3interpolation improve the biomass of plant, thus significantly improve the repairing effect of plant to lead-contaminated soil, wherein with 1 μM of GA
3most effective with the compound action of EDTA.
If be 3. used alone 100 μMs of GA
3not only suppress plant growth, and significantly reduce concentration plumbous in plant, but with 100 μMs of GA
3still better to the repairing effect of lead-contaminated soil with plant under the compound action of EDTA.
Accompanying drawing explanation
The different disposal that Fig. 1 provides for the embodiment of the present invention 1 is to the detection figure of rye grass biomass;
The different disposal that Fig. 2 provides for the embodiment of the present invention 2 is to the detection figure of rye grass biomass;
The different disposal that Fig. 3 provides for the embodiment of the present invention 3 is to the detection figure of rye grass biomass.
Detailed description of the invention
Below in conjunction with accompanying drawing, the present invention is further analyzed.
Embodiment 1
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 250mg/kg.Research finds, compared with disposable interpolation, during divided dose interpolation EDTA, plant is to the extraction of Lead In Soil and larger (the document 4:Hadi of transhipment amount, F., Bano, A., Fuller, M.P.2010.The improvedphytoextraction of lead (Pb) and the growth of maize (Zea mays L.): the role of plant growth regulators (GA
3and IAA) and EDTA alone andin combinations [J] .Chemosphere80,457-462.).And, when EDTA concentration is 2.5mmol/kg, buckwheat and sunflower to the absorption of lead and turn-over capacity the strongest, therefore in this experiment, EDTA concentration is set to 2.5mmol/kg(document 5:Chen, Y.H., Li, X.D., et al., 2004.Leaching and uptake of heavy metals by ten different species ofplants during an EDTA-assisted phytoextraction process [J] .Chemosphere57,187-196.).Fill basin after crossing 5mm sieve for examination soil, every basin 18.5cm (diameter) × 21cm (height) fills native 2kg (dry weight), basin underlay pallet.For Pb in examination soil with Pb (NO
3)
2the form of the aqueous solution adds, and fully mixes, and recording Lead In Soil concentration is 250mg/kg, then adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), 2.5mmol/kg EDTA (T1) is added, foliar spray 1 μM of GA
3(T2), foliar spray 10 μMs of GA
3(T3), foliar spray 100 μMs of GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μM of GA
3(T5), 2.5mmol/kg EDTA+ foliar spray 10 μMs of GA
3(T6), 2.5mmol/kgEDTA+ foliar spray 100 μMs of GA
3(T7).Each experimental group all establishes 3 repetitions.Experimental group C in contrast.After ryegrass seed germinates 7 days, carry out GA
3with the interpolation alone or in combination of EDTA, carry out GA week about
3foliar spray is or/and EDTA directly adds in soil.GA
3be all 5mL with each addition of EDTA, add 4 times altogether.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, repeatedly rinses plant with running water, finally blot with the water of blotting paper by surface.Dry at 75 DEG C to weight, pulverize for subsequent use after weighing dry weight.
Rye grass biomass variety as shown in Figure 1.As seen from Figure 1, except EDTA processes separately, other process all promote the growth of rye grass, show as the increase of biomass.Compared with the control, EDTA processes down separately, and rye grass Aboveground Biomass of Young have dropped 19.4 ﹪.Compared with the control, experimental group T2, T3 make the biomass of rye grass add 13.3 ﹪ ~ 45.1 ﹪, wherein at 10 μMs of GA
3during process, phytomass reaches maximum.On the contrary, foliar spray 100 μMs of GA
3significantly suppress the growth of plant.Interpolation EDTA and concentration are the GA of 1 μM or 10 μMs simultaneously
3, the biomass of plant is along with GA
3the increase of concentration and increasing, compared with the control, the biomass of rye grass adds 1.5 ﹪ ~ 25.2 ﹪, but with single concentration GA
3process is compared, and under Combined Processing, rye grass biomass declines.But at EDTA+100 μM of GA
3under process, phytomass reduces 28.7 ﹪ than contrast.Above result of study shows, concentration is the GA of 1 μM or 10 μMs
3effectively to alleviate in soil EDTA to the suppression of Ryegrass Growth, but 100 μMs of GA
3inhibitory action is revealed to the growth table of rye grass.
Pb content in rye cursive script under table 1 different disposal
Table 1 can be found out, different disposal all promotes that rye grass is to the extraction of Pb and enrichment.Concentration is the GA of 1 μM or 10 μMs
3significantly promote rye grass root and aerial part to the absorption of Pb, compared with the control, root and aerial part Pb content add 11.4 ﹪ ~ 26.1 ﹪ and 48.7 ﹪ ~ 99.1 ﹪ respectively, wherein with 1 μM of GA
3act on the most remarkable.10 μMs of GA
3root and aerial part Pb content is made to reduce 18.7 ﹪ and 32.1 ﹪ respectively.EDTA and GA
3combined Processing extracts rye grass Pb and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration add 1.4 ~ 2.1 and 5.8 ~ 8.1 times respectively compared with the control.Wherein under T5 experimental group, plant parts Pb reaches maximum, and root and aerial part reach 802.1mg/kg and 500.6mg/kg respectively, and its aerial part Pb content is significantly higher than other experimental group (P<0.05).
Table 2 different disposal is on the impact of plant absorption Pb
From table 2, except T4 experimental group, the rye grass of other experimental group all significantly improves the enrichment of Pb and transhipment coefficient.Concentration is the GA of 1 μM or 10 μMs
3under process, the enrichment of Pb in rye cursive script and transhipment coefficient rise with the increase of concentration; But 100 μMs of GA
3the enrichment of rye cursive script lead and transhipment coefficient is made to reduce 31.6 ﹪ and 18.2 ﹪ respectively.Compared with the control, GA
3combine with EDTA to add and promote that plant is to the extraction of Pb and enrichment, plant adds 5.8 ~ 8.5 and 1.8 times respectively to the enrichment of Pb and transhipment coefficient, wherein the most remarkable with the process effect of T5 experimental group.
Table 3 different disposal is on the impact of plant Pb extracted amount and extraction efficiency
Rye grass is 0.06 ﹪ ~ 1.1 ﹪ (table 3) to the extraction efficiency of Pb.Except T4 experimental group, GA
3separately or all significantly promote the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3.In different disposal, under the process of T5 experimental group, rye grass reaches maximum to the extracted amount of Pb and extraction efficiency, and this process is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μM or 10 μMs
3significantly improve the biomass of plant with EDTA synergy, promote that plant is to the absorption of Pb and accumulation.Wherein, T5 experimental group is best suited for strengthening rye grass remediating lead-contaminated soil.But 100 μMs of GA
3independent role all plays inhibitory action to Ryegrass Growth and plumbous enrichment.
Embodiment 2
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 500mg/kg.Fill basin after crossing 5mm sieve for examination soil, every basin 18.5cm (diameter) × 21cm (height) fills native 2kg (dry weight), basin underlay pallet.For examination P in soil b with Pb (NO
3)
2the form of the aqueous solution adds soil, and fully mixes, and recording Lead In Soil concentration is 500mg/kg, adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), 2.5mmol/kg EDTA (T1) is added, foliar spray 1 μM of GA
3(T2), foliar spray 10 μMs of GA
3(T3), foliar spray 100 μMs of GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μM of GA
3(T5), 2.5mmol/kg EDTA+ foliar spray 10 μMs of GA
3(T6), 2.5mmol/kg EDTA+ foliar spray 100 μMs of GA
3(T7).Each experimental group all establishes 3 repetitions.Experimental group C in contrast.After ryegrass seed germinates 7 days, carry out GA
3with the interpolation alone or in combination of EDTA, carry out GA week about
3foliar spray is or/and EDTA directly adds in soil.GA
3be all 5mL with each addition of EDTA, add 4 times altogether.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, repeatedly rinses plant with running water, finally blot with the water of blotting paper by surface.Dry at 75 DEG C to weight, pulverize for subsequent use after weighing dry weight.
Rye grass biomass variety as shown in Figure 2.As seen from Figure 2, except EDTA process, different disposal all promotes the growth of rye grass, shows as the increase of biomass.Compared with the control, EDTA processes down separately, and rye grass Aboveground Biomass of Young have dropped 16.3 ﹪.Foliar spray concentration is the GA of 1 μM or 10 μMs
3make the biomass of rye grass add 18.7 ﹪ ~ 52.5 ﹪, wherein when the process of T2 experimental group, phytomass reaches maximum.But 100 μMs of GA
3significantly suppress the growth of plant.Interpolation EDTA and concentration are the GA of 1 μM or 10 μMs simultaneously
3, make the biomass of rye grass add 9.9 ﹪ ~ 31.2 ﹪, but with single concentration GA
3process is compared, and under Combined Processing, rye grass biomass declines.On the contrary, 100 μMs of GA
3+ EDTA makes rye grass biomass reduce 24.2 ﹪.Above result of study shows, concentration is the GA of 1 μM or 10 μMs
3effectively to alleviate in soil EDTA to the suppression of Ryegrass Growth, but 100 μMs of GA
3inhibitory action is revealed to the growth table of rye grass.
Pb content in rye cursive script under table 4 different disposal
Table 4 can be found out, different disposal all promotes that rye grass is to the extraction of Pb and enrichment.Concentration is the GA of 1 μM or 10 μMs
3significantly promote rye grass root and aerial part to the absorption of Pb, compared with the control, root and aerial part Pb content add 17.6 ﹪ ~ 29.2 ﹪ and 41.4 ﹪ ~ 116.3 ﹪ respectively, wherein with 1 μM of GA
3act on the most remarkable.On the contrary, 100 μMs of GA
3rye grass root and aerial part lead concentration is made to reduce 23.3 ﹪ and 25.7 ﹪ respectively.EDTA and GA
3combined Processing extracts rye grass Pb and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration add 1.2 ~ 2.0 and 5.0 ~ 7.2 times respectively compared with the control.Wherein under the process of T5 experimental group, plant parts Pb reaches maximum, and root and aerial part reach 1654.5mg/kg and 1250.6mg/kg respectively, and its aerial part Pb content is significantly higher than other process (P<0.05).
Table 5 different disposal is on the impact of plant absorption Pb
From table 5, except 100 μMs of GA
3outside independent processed group, under different disposal, rye grass all significantly improves the enrichment of Pb and transhipment coefficient.Compared with the control, foliar spray concentration is the GA of 1 μM or 10 μMs
3the enrichment of Pb in rye cursive script and transhipment coefficient is made to improve 0.4 ~ 1.2 and 0.2 ~ 0.6 times.Compared with the control, GA
3combine with EDTA to add and promote that plant is to the extraction of Pb and enrichment, plant adds 5.1 ~ 7.2 and 1.7 times respectively to the enrichment of Pb and transhipment coefficient, wherein the most remarkable with the lower effect of T5 process.
Table 6 different disposal is on the impact of plant Pb extracted amount and extraction efficiency
Rye grass is 0.08 ﹪ ~ 1.3 ﹪ (table 6) to the extraction efficiency of Pb.Concentration is the GA of 1 μM or 10 μMs
3separately or all significantly promote the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3, but 100 μMs of GA
3independent role makes the extracted amount of rye grass lead and extraction efficiency reduce 34.6 ﹪ and 33.3 ﹪ respectively.Under T5 process, rye grass reaches maximum to the extracted amount of Pb and extraction efficiency, and this process is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μM or 10 μMs
3significantly improve the biomass of plant with EDTA synergy, promote that plant is to the absorption of Pb and accumulation.Wherein, the process of T5 experimental group is best suited for strengthening rye grass remediating lead-contaminated soil.But 100 μMs of GA
3independent role inhibits the growth of plant and it is to the extraction of lead and enrichment.
Embodiment 3
Experiment place is located in greenhouse, Zhejiang Prov Industrial And Commercial University Environmental Studies Institute, and pot experiment soil picks up from farmland, countryside, Hangzhou, and soil types is clay.The lead concentration of soil used is 750mg/kg.Basin is filled, every basin 18.5cm(diameter after crossing 5mm sieve for examination soil) × 21cm(is high) fill native 2kg (dry weight), basin underlay pallet.For Pb in examination soil with Pb (NO
3)
2the form of the aqueous solution adds soil, and fully mixes, and the lead concentration recording soil is 750mg/kg, then adds water to 75 ﹪ of field capacity, stablizes after 30 days for test in greenhouse.8 experimental group are established in experiment altogether, are respectively: do not add EDTA and GA
3(C), 2.5mmol/kg EDTA (T1) is added, foliar spray 1 μM of GA
3(T2), foliar spray 10 μMs of GA
3(T3), foliar spray 100 μMs of GA
3(T4), 2.5mmol/kg EDTA+ foliar spray 1 μM of GA
3(T5), 2.5mmol/kgEDTA+ foliar spray 10 μMs of GA
3(T6), 2.5mmol/kg EDTA+ foliar spray 100 μMs of GA
3(T7).Each experimental group all establishes 3 repetitions.Experimental group C in contrast.After ryegrass seed germinates 7 days, carry out GA
3with the interpolation alone or in combination of EDTA, carry out GA week about
3foliar spray is or/and EDTA directly adds in soil.GA
3be all 5mL with each addition of EDTA, add 4 times altogether.
The 42nd day herborization sample after rye grass germinates, is divided into root and aerial part by the plant sample of results, repeatedly rinses plant with running water, finally blot with the water of blotting paper by surface.Dry at 75 DEG C to weight, pulverize for subsequent use after weighing dry weight.
Rye grass biomass variety as shown in Figure 3.As seen from Figure 3, except EDTA process, different disposal all promotes the growth of rye grass, shows as the increase of biomass.Compared with the control, EDTA processes down separately, and rye grass Aboveground Biomass of Young have dropped 11.7 ﹪.Compared with the control, concentration is the GA of 1 μM or 10 μMs
3independent process makes the biomass of rye grass add 26.1 ﹪ ~ 62.5 ﹪, and wherein when the process of T2 experimental group, phytomass reaches maximum, but 100 μMs of GA
3process makes rye grass biomass reduce.Interpolation EDTA and concentration are the GA of 1 μM or 10 μMs simultaneously
3, the biomass of plant is along with GA
3the increase of concentration and increasing.Compared with the control, the biomass of rye grass adds 14.1 ﹪ ~ 40.3 ﹪, but with single concentration GA
3process is compared, and under Combined Processing, rye grass biomass declines.Above result of study shows, concentration is the GA of 1 μM or 10 μMs
3effectively to alleviate in soil EDTA to the suppression of Ryegrass Growth.On the contrary, 100 μMs of GA
3separately or all significantly suppress the growth of rye grass with EDTA synergy.
Pb content in rye cursive script under table 7 different disposal
Table 7 can be found out, different disposal all promotes that rye grass is to the extraction of Pb and enrichment.Concentration is the GA of 1 μM or 10 μMs
3significantly promote rye grass root and aerial part to the absorption of Pb, compared with the control, root and aerial part Pb content add 0.5 ﹪ ~ 8.5 ﹪ and 15.0 ﹪ ~ 46.1 ﹪ respectively, wherein with 1 μM of GA
3act on the most remarkable.On the contrary, 100 μMs of GA
3independent process makes rye grass root and aerial part lead concentration reduce 23.4 ﹪ and 17.1 ﹪ respectively.EDTA and GA
3combined Processing extracts rye grass Pb and collaborative facilitation is played in enrichment, and root and aerial part Pb concentration add 1.0 ~ 1.6 and 3.4 ~ 5.6 times respectively compared with the control.Wherein under T5 process, plant parts Pb reaches maximum, and root and aerial part reach 2430.5mg/kg and 2452.4mg/kg respectively, and its aerial part Pb content is significantly higher than other process (P<0.05).
Table 8 different disposal is on the impact of plant absorption Pb
From table 8, except 100 μMs of GA
3outside independent processed group, under different disposal, rye grass all significantly improves the enrichment of Pb and transhipment coefficient.Compared with the control, GA
3combine with EDTA to add and promote that plant is to the extraction of Pb and enrichment, plant adds 3.5 ~ 5.6 and 1.3 ~ 1.5 times respectively to the enrichment of Pb and transhipment coefficient, wherein the most remarkable with the lower effect of T5 process.
Table 9 different disposal is on the impact of plant Pb extracted amount and extraction efficiency
Rye grass is 0.13 ﹪ ~ 1.5 ﹪ (table 9) to the extraction efficiency of Pb.Except 100 μMs of GA
3outside independent processed group, GA
3separately or all significantly promote the remediation efficiency of rye grass to Pb with EDTA synergy, its facilitation is GA
3+ EDTA > EDTA > GA
3.Under the process of T5 experimental group, rye grass reaches maximum to the extracted amount of Pb and extraction efficiency, and this process is conducive to rye grass most and repairs Pb contaminated soil.
Above-mentioned experimental result shows, concentration is the GA of 1 μM or 10 μMs
3significantly improve the biomass of plant with EDTA synergy, promote that plant is to the absorption of Pb and accumulation.Wherein, the process of T5 experimental group is best suited for strengthening rye grass remediating lead-contaminated soil.On the contrary, 100 μMs of GA
3independent role inhibits the growth of plant and it is to the extraction of lead and enrichment.
Claims (1)
1. the method for gibberellin, the combined reinforced remediating lead-contaminated soil of ethylenediamine tetra-acetic acid, is characterized in that the method specifically plants rye grass in lead-contaminated soil to be repaired, after plant germination the 7th day, foliar spray GA
3solution, in soil, add EDTA solution simultaneously, carry out 4 process altogether, the interval time of each process is 7 days, within 42 days after plant germination, plant entirety is removed, then plants second batch plant, repeat aforesaid operations, until the lead content in soil reaches environmental safety standard, thus reach the object of remediating lead-contaminated soil;
Regularly water in growing process, remain that soil moisture content is 75 ﹪ of maxmun field capacity;
GA
3the concentration of solution is 1 μM, 10 μMs or 100 μMs;
The concentration of EDTA solution is 2.5mmol/kg;
Each processing procedure GA
3the addition of solution and EDTA solution is 5mL.
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