CN104138894A - Cadmium-contaminated soil enhanced repairing agent and application - Google Patents
Cadmium-contaminated soil enhanced repairing agent and application Download PDFInfo
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- CN104138894A CN104138894A CN201310167642.6A CN201310167642A CN104138894A CN 104138894 A CN104138894 A CN 104138894A CN 201310167642 A CN201310167642 A CN 201310167642A CN 104138894 A CN104138894 A CN 104138894A
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Abstract
The invention relates to a phytoremediation technology of cadmium-contaminated soil, specifically to a cadmium-contaminated soil enhanced repairing agent and its application. The enhanced repairing agent is levorotary reduced glutathione. Weighed according to mole ratio, concentration of the levorotary reduced glutathione is 0.1-1.0 mmol/kg. According to the invention, 0.1-1.0mmol/kg of the levorotary reduced glutathione in the form of a water mixture is poured into contaminated soil where solanum nigrum is planted. The levorotary reduced glutathione is poured at a time in the seedling stage of solanum nigrum; afterwards, the levorotary reduced glutathione is poured normally according to water shortage condition every day to make water content of the soil reach 80% of the maximum water-holding capacity; when solanum nigrum ripens, the plant and a soil sample are gained. In comparison with a contrast without the addition of amino acid, high doses of a levorotary reduced glutathione water mixture has the optimum processing effect. Cd content of solanum nigrum above the ground is raised by 24.9-36.2%, and reparing efficiency of cadmium by solanum nigrum is remarkably improved.
Description
Technical field
The present invention relates to the phytoremediation technology of cadmium pollution soil, specifically a kind of cadmium pollution soil strengthening renovation agent and application thereof.
Background technology
Along with the variation of pollutant emission, complicated and frequentization, the contaminated degree of major receptors soil of pollutant increases the weight of day by day, the complexity that contaminated soil is repaired and refractory rationality feature be outstanding all the more (document 1:Paton GI also, Viventsova E, Kumpene J, et al.2006.Anecotoxicity assessment of contaminated forest soils from the Kola Peninsula.Science of the Total Environment, 355 (13): 106-117), it is more common pollution type that wherein heavy metal-organic composite pollutes, as Cd-polycyclic aromatic hydrocarbon (Polycyclic aromatic hydrocarbons, PAHs) combined pollution.Cadmium can be by reducing photosynthesis of plant in the time of low concentration, produce the approach such as oxidative stress suppress plant growth, to plant produce toxic action (document 2:Schill RO,
h,
hR.2003.Laboratory simulation of a mining accident:acute toxicity hsc/hsp70response and recovery from sress in Gammarus fossarum (Crustacea smphipoda) exposed to a pulse of cadmium.Biometals, 16:391-401), and to some extent humans and animals is produced to toxic action, also can bring out canceration; And PAHs is a class persistence organic pollutant, biology is had to chronic toxicity and carcinogenic, teratogenesis, mutagenicity.Have disguise and chronicity because Cd, PAHs show as in soil environment, cause the difficulty of repairing this type of combined contamination soil to strengthen, seeking efficient, safe, economic recovery technique becomes the realistic problem of needing solution badly.Phytoremediation technology have the incomparable expense of physics, chemical repair method cheap, be difficult for causing secondary pollution, beautify the environment, be easy to as advantages such as society are accepted; become extremely potential recovery technique (document 3:Chaney RL; Malik M; Li YM.1997.Phytoremediation of soil metals.Current Opinions in Biotechnology, 8:279-284; Brooks RR, Chambers MF, Nickls L J, et al.1998.Phytomining.Trends in Plant Science, 3 (9): 359-362).The phytoremediation of heavy-metal contaminated soil is mainly to be removed and be used for realizing (document 4: Wei Shuhe by the excess of super enriching plant or enriching plant, Zhou Qixing, Pavel V.Koval, Galina A.Belogolova. Phytoremediation of organic pollutants-contaminated environment: A research review. ecological magazine .2006,25 (6): 716-721); For organic contamination as polycyclic aromatic hydrocarbon be mainly to utilize the degradation of plant rhizosphere circle microorganism to complete (document 4: Wei Shuhe, Zhou Qixing, Pavel V.Koval, Galina A.Belogolova. Phytoremediation of organic pollutants-contaminated environment: A research review. ecological magazine .2006,25 (6): 716-721).But it is restricted with some that phytoremediation technology also exists some defects, as being very easily subject to, the own biological characteristics of environmental condition and plant affects, repairing efficiency is long etc.Therefore need to adopt some strengthening reclamation activities to improve the efficiency of phytoremediation, to improving reference is provided early for this technology.
Under Heavy Metal Contamination Stress; a lot of plants have formed certain self-protective mechanism resists the harm of heavy metal ion; there is the little molecule (citrate of complexation heavy metal ion ability as biosynthesis is a series of; glutathione; plant complexing element etc.); form with free complex the compound that toxicity is lower, and be stored in the vacuole of plant cell.Wherein plant complexing element is important removing toxic substances material in plant. plant complexing element is that heavy metal is as the class sulfydryl chelating polypeptide producing in cytoplasm liquid under the stress-inducings such as Ni, Cd, Zn, Te, Au, Hg, Pb, As, Se, wherein Cd is the strongest interior inducible factor of body, and performance inducing action is metal ion (document 5:Rauser WE.1995.Phytochelatins and related peptides.Plant Physiology, 109:1141-1149) free in cytoplasm.PCs (Phytochelatins) is the polypeptide that a class is rich in cysteine (cysteine), is that original substrate generates through a series of enzymatic reactions by cysteine, glutamic acid, glycine.Cys sulfydryl in PCs can with born of the same parents in cd
2+form coordinate bond, and then formation PCs-Cd compound, this compound greatly reduces the toxicity of cell, and plant has also further taked vacuole compartmentation (compartmentation) that compound is fixed in vacuole in addition, has further reduced the toxic action of Cd to plant.
Summary of the invention
The object of the present invention is to provide a kind of result of use is good, can improve phytoremediation efficiency cadmium pollution soil strengthening renovation agent and application thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of cadmium pollution soil strengthening renovation agent, strengthening renovation agent is left-handed reduced glutathione, wherein, by the concentration applying is by mol left-handed reduced glutathione=0.1-1.0mmol/kg.
The application of cadmium pollution soil strengthening renovation agent, using left-handed reduced glutathione as super enriching plant and/or the strengthening renovation agent of enriching plant, for cadmium pollution soil repair.
The add concentration of the left-handed reduced glutathione of described strengthening renovation agent in contaminated soil is 0.1-1.0mmol/kg soil.
The add concentration of described strengthening renovation agent in contaminated soil is the left-handed reduced glutathione that every kg soil adds 0.1-1.0mmol; Its soil is to measure with the soil weight of the 0-20 centimetre of dark soil thickness in soil layer surface.
The content 0.3-100mg/kg of described Heavy Metals in Contaminated Soils Cd.Described super enriching plant and/or enriching plant are black nightshade.
The present invention has advantages of: the present invention mixes and pours into left-handed reduced glutathione with the form of water mixed liquid by the content of 0.1-1.0mmol/kg on the contaminated soil of plantation black nightshade, black nightshade disposable pouring in seedling stage, normally water inferior depending on lack of water situation every day afterwards, make the water content of soil reach 80% of maximum water-holding capacity, in the time that black nightshade is ripe, results plants and soil sample, with do not add amino acid whose contrast and compare, left-handed reduced glutathione mixed liquor treatment effect the best of high dose, black nightshade overground part Cd content has improved 24.9%-36.2%, improve significantly the remediation efficiency of black nightshade to cadmium.
Detailed description of the invention
The strengthening repair of the left-handed reduced glutathione of embodiment to cadmium pollution soil
1. test material
According to the Northeast's Heavy Metal Pollution and level, determine Cd Single Pollution processing test, it is 10mg/kg that Cd adds concentration, is equivalent to 10 times of national standard of soil environment quality (GB15618,1995) grade III Standard value.The Heavy Metals adding is respectively CdCl
22.5H
2o is guaranteed reagent, joins in soil with solid-state, fully mixes. meanwhile, be treated to contrast (CK) with what do not add heavy metal.Rehabilitation plant is black nightshade (Solanum nigrum), and seed picks up from ecological experiment station, Chinese Academy of Sciences Shenyang agricultural soil.Left-handed reduced glutathione is guaranteed reagent.
2. test method
According to the synthetic level of left-handed reduced glutathione in plant and the effect in detoxification processes in plant thereof, experimental design 3 kinds of differences that left-handed reduced glutathione applies seedling stage, apply and all apply in seedling stage and florescence florescence put add mode, form with water mixed liquid pours into, adding concentration range is 0.1-1.0mmol/kg, be divided into two levels of low dosage 0.4mmol/kg and high dose 0.8mmol/kg, specifically in table 1.Separately establish 1 contrast that does not add left-handed reduced glutathione, totally 7 processing, every processing repeats 3 times, adopts liquid to add method, once adds.
Test is carried out in greenhouse.To, for the air-dry rear 4mm sieve of crossing of examination soil, accurately weigh 2.5kg soil and pack plastic tub into; Black nightshade seed is seeded in to Miao Panzhong, in the time that black nightshade seedling grows to 3-5 sheet spire, selects the consistent black nightshade seedling of growth to move into respectively in each processing basin, every potted plant 6 seedling.Control the temperature 25-30 of culturing room DEG C, air humidity 50%-80%, intensity of illumination 350-450umol/m
2s, light application time 08:00-17:30.Transplant after 15 days, in the time that seedling grows to 10cm left and right, corresponding each processing adds respectively, and irregularly waters running water according to basin lack of water situation, makes soil moisture content often remain on 80% left and right of field capacity.For preventing the filter loss for the treatment of fluid leaching, under basin, place plastic pallet and in time effusion refunded in basin.After 80 days in plant maturity sampling (in the time that black nightshade is ripe, results plants and soil sample).
The left-handed reduced glutathione processing horizontal of table 1 table (mmol/kg)
| Process | Left-handed reduced glutathione |
| CK | 0 |
| T1 | Low dosage |
| T2 | High dose |
| T3 | Low dosage |
| T4 | High dose |
| T5 | Low dosage+low dosage |
| T6 | High dose+high dose |
3. sample analysis
A plant sample part for results is for the mensuration of overground part content of beary metal, and remainder root division, stem, leaf, fruit 4 parts, fully rinse with running water respectively, and then with deionized water rinsing, drain moisture, the 30min that completes at 105 DEG C then dries to constant weight at 70 DEG C in baking oven.Plant sample after oven dry pulverizes and sieves for subsequent use, and air-dry rear mistake 100 mesh sieves of pedotheque are for subsequent use.Plant and pedotheque all adopt HNO
3-HClO
4method digestion (the two volume ratio is 3:1), atomic absorption spectrophotometer method is measured the content of Cd, and atomic absorption spectrophotometer is the 180-80 of Hitachi, and the absorbing wavelength of cadmium is 228.8.The mensuration of the basic physical and chemical such as the content of organic matter of soil adopts conventional assay method.
4. data statistics
The data that obtain are carried out statistical analysis with Microsoft Excel and SPSS16.0, utilize Duncan newly to answer range test and carry out significance test of difference (p<0.05).
5. result
5.1 impacts of left-handed reduced glutathione on black nightshade growth characteristics
Table 2 has provided black nightshade root and the upperground part biomass situation of change under indoor pot condition.From the appearance, under Cd pollutional condition, black nightshade normal growth, there is not minus green, the stress symptom that is subject to such as withered and yellow in blade.Compare with not adding contrasting of left-handed reduced glutathione, the processing of the each concentration level of left-handed reduced glutathione has remarkable impact (p<0.05) to plant dry weight, and wherein T2 processes and improved more than 20%.
The impact of the left-handed reduced glutathione of table 2 on black nightshade biomass
| Process | Root | Overground part |
| CK | 0.21 | 2.43 |
| T1 | 0.25 | 2.85 |
| T2 | 0.26 | 3.05 |
| T3 | 0.23 | 2.76 |
| T4 | 0.24 | 2.99 |
| T5 | 0.23 | 3.03 |
| T6 | 0.25 | 3.38 |
5.2 left-handed reduced glutathiones are on absorbing the impact of Cd in black nightshade body
Table 3 has provided different left-handed reduced glutathiones and has processed lower black nightshade root, stem, leaf, 4 part Cd content of fruit.As can be seen from Table 3, compared with the control, the content of respectively processing root, stem, fruit and overground part has increase in various degree, and wherein T2 processes the amount maximum increasing, and is up to 36.2%.
The impact (mg/kg) of the left-handed reduced glutathione of table 3 on Cd accumulation in black nightshade body
| Process | Root | Stem | Leaf | Really |
| CK | 93.2 | 96.7 | 159.9 | 23.9 |
| T1 | 105.6 | 120.7 | 184.8 | 27.8 |
| T2 | 121.0 | 131.7 | 199.8 | 32.3 |
| T3 | 101.6 | 109.1 | 176.6 | 26.6 |
| T4 | 106.9 | 126.4 | 188.3 | 27.7 |
| T5 | 100.7 | 102.2 | 163.1 | 24.1 |
| T6 | 102.6 | 116.8 | 171.4 | 25.8 |
Claims (6)
1. a cadmium pollution soil strengthening renovation agent, is characterized in that: strengthening renovation agent is left-handed reduced glutathione, wherein, counts left-handed reduced glutathione=0.1-1.0mmol/kg by the concentration applying mole.
2. an application for cadmium pollution soil claimed in claim 1 strengthening renovation agent, is characterized in that: using left-handed reduced glutathione as super enriching plant and/or the strengthening renovation agent of enriching plant, for cadmium pollution soil repair.
3. by the application of cadmium pollution soil strengthening renovation agent claimed in claim 2, it is characterized in that: the add concentration of the left-handed reduced glutathione of described strengthening renovation agent in contaminated soil is 0.1-1.0mmol/kg soil.
4. by the application of cadmium pollution soil strengthening renovation agent described in claim 3, it is characterized in that: the add concentration of described strengthening renovation agent in contaminated soil is the left-handed reduced glutathione that every kg soil adds 0.1-1.0mmol; Its soil is to measure with the soil weight of the 0-20 centimetre of dark soil thickness in soil layer surface.
5. by the application of cadmium pollution soil strengthening renovation agent described in claim 2, it is characterized in that: the content 0.3-100mg/kg of described Heavy Metals in Contaminated Soils Cd.
6. according to the application of cadmium pollution soil strengthening renovation agent described in claim 2, it is characterized in that: described super enriching plant and/or enriching plant are black nightshade.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104472175A (en) * | 2014-12-01 | 2015-04-01 | 四川农业大学 | Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum |
| CN104858226A (en) * | 2015-06-12 | 2015-08-26 | 南开大学 | Method for repairing heavy-metal cadmium polluted soil by using bougainvillea speetabilis plants |
| CN105368460A (en) * | 2015-12-08 | 2016-03-02 | 江苏盖亚环境工程有限公司 | Lead metal soil pollution nanometer repairing agent and preparation method thereof |
| CN105600975A (en) * | 2015-10-30 | 2016-05-25 | 江南大学 | Method for immobilizing cadmium ions in water through using rice proteins |
| CN108296276A (en) * | 2018-01-04 | 2018-07-20 | 华南农业大学 | A method of the Rapid deactivation Cadmium in Soil based on amino-acid complex reallocation principle |
| CN109794499A (en) * | 2019-02-01 | 2019-05-24 | 中国科学院南京土壤研究所 | Application of the cold plasma method for treating seeds in fortification of plants cadmium pollution soil repair |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104472175A (en) * | 2014-12-01 | 2015-04-01 | 四川农业大学 | Method for improving cadmium-contaminated orchard soil remediation capability of solanum photeinocarpum |
| CN104858226A (en) * | 2015-06-12 | 2015-08-26 | 南开大学 | Method for repairing heavy-metal cadmium polluted soil by using bougainvillea speetabilis plants |
| CN105600975A (en) * | 2015-10-30 | 2016-05-25 | 江南大学 | Method for immobilizing cadmium ions in water through using rice proteins |
| CN105600975B (en) * | 2015-10-30 | 2017-12-12 | 江南大学 | A kind of method for solidifying cadmium ion in water using rice protein |
| CN105368460A (en) * | 2015-12-08 | 2016-03-02 | 江苏盖亚环境工程有限公司 | Lead metal soil pollution nanometer repairing agent and preparation method thereof |
| CN108296276A (en) * | 2018-01-04 | 2018-07-20 | 华南农业大学 | A method of the Rapid deactivation Cadmium in Soil based on amino-acid complex reallocation principle |
| CN109794499A (en) * | 2019-02-01 | 2019-05-24 | 中国科学院南京土壤研究所 | Application of the cold plasma method for treating seeds in fortification of plants cadmium pollution soil repair |
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