CN115211334A - Application method of leaf surface blocking and controlling agent of L-cysteine in Chinese cabbage planting - Google Patents
Application method of leaf surface blocking and controlling agent of L-cysteine in Chinese cabbage planting Download PDFInfo
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- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 title claims abstract description 82
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 title claims abstract description 67
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 56
- 235000013878 L-cysteine Nutrition 0.000 title claims abstract description 41
- 239000004201 L-cysteine Substances 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 20
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 title claims abstract description 19
- 230000000903 blocking effect Effects 0.000 title description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 title 1
- 244000221633 Brassica rapa subsp chinensis Species 0.000 claims abstract description 66
- 238000005507 spraying Methods 0.000 claims abstract description 57
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 51
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 51
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims abstract description 22
- 229930002875 chlorophyll Natural products 0.000 claims abstract description 17
- 235000019804 chlorophyll Nutrition 0.000 claims abstract description 17
- 239000002689 soil Substances 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000012546 transfer Methods 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 3
- 229930002868 chlorophyll a Natural products 0.000 claims description 5
- 229930002869 chlorophyll b Natural products 0.000 claims description 5
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 239000003595 mist Substances 0.000 claims description 4
- 230000005764 inhibitory process Effects 0.000 claims description 3
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 abstract 1
- 229960003067 cystine Drugs 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 235000021384 green leafy vegetables Nutrition 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 240000007124 Brassica oleracea Species 0.000 description 3
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 3
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 230000000979 retarding effect Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 229960001471 sodium selenite Drugs 0.000 description 2
- 235000015921 sodium selenite Nutrition 0.000 description 2
- 239000011781 sodium selenite Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
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- 238000006243 chemical reaction Methods 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 230000004792 oxidative damage Effects 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 229940091258 selenium supplement Drugs 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention discloses a use method of a leaf surface resistance control agent of L-cysteine in Chinese cabbage planting, which comprises the following steps: step one, fully stirring and dissolving L-cysteine at a certain water temperature to prepare 10mmol L ‑1 、20mmol L ‑1 And 30mmol L ‑1 L-half of three concentrationsCollecting and storing the cystine solution to obtain the leaf surface resistance and control agent; step two, spraying a first foliage resistance and control agent after the Chinese cabbages grow to the three-leaf and one-heart; and step three, spraying is also carried out on 7 th and 14 th days after the first spraying, and spraying is carried out for three times. After the foliage resistance and control agent is sprayed on the leaves of the pakchoi, the invention can effectively prevent and control the transfer of cadmium to the edible parts of the pakchoi and can effectively reduce medium and light cadmium-polluted soil (the cadmium content is less than or equal to 1.5mg kg) ‑1 ) Cadmium content of the medium and small Chinese cabbages; using 30mmol L ‑1 The L-cysteine with concentration is sprayed in the three-leaf one-heart period of the pakchoi, so that the chlorophyll content in the pakchoi can be increased.
Description
Technical Field
The invention relates to the technical field of heavy metal pollution farmland treatment, in particular to a use method of a leaf surface resistance and control agent of L-cysteine in Chinese cabbage planting.
Background
Arable soil is a natural resource on which humans rely for survival. In recent years, with the progress of industrial activities and the increase in the amount of agricultural input, a large amount of heavy metals such as cadmium are released into soil. According to the first national soil pollution condition survey bulletin result, the cadmium pollution overproof point is the most, and reaches 7% of all overproof points. Cadmium in soil has the characteristics of long decomposition period, high mobility, difficult degradation and the like, can not only produce toxic action on plants, but also concentrate and amplify along with food chains, and finally can produce harm to human health. Therefore, the method effectively prevents and controls the accumulation of cadmium in agricultural products and is an important technical measure for ensuring the food safety.
China is the first major country for vegetable production and consumption in the world, and the cadmium enrichment effect of leaf vegetables is obviously higher than that of other vegetables. The pakchoi is one of leaf vegetables with high enrichment on cadmium. Even in the soil with slight cadmium pollution, the pakchoi still has higher cadmium enrichment amount. Therefore, the safe production of pakchoi is not negligible.
Among numerous heavy metal resistance control technologies, passivation remediation has the characteristics of good resistance control effect, simplicity in operation and the like, and is suitable for remediation of large-area polluted farmlands, but long-term application of the passivator can destroy the granular structure of soil, cause soil hardening, and further influence the growth of crops.
The leaf surface resistance control technology is considered as an effective means for reducing the cadmium accumulation of crops, has the characteristics of high efficiency, rapidness and safety, and has the potential of large-area popularization and application. Many non-metal, metal and organic materials used as the foliage resistance and control agent show good cadmium reduction effect. However, some of these inhibitors are not effective and even present an environmental risk. For example, sodium selenite is used as a foliage spray of grapes, the content of organic selenium in plant autolysate is low, the biological activity is low, and the toxic and side effects are great. The active sulfhydryl group contained in the L-cysteine has reducibility and chemical reaction activity, plays a key role in the biosynthesis of proteins and cell membranes, can protect cells from oxidative damage, and plays a plurality of important roles in organisms.
Most of the existing foliage resistance control agents are mainly applied to grain crops such as rice, wheat and the like, and the methods for directly spraying the foliage resistance control agents on edible leaf vegetables are still few. Chinese patents with publication numbers CN104478555a and CN111763119a respectively disclose a method for preparing a foliar retarding and controlling agent by using L-cysteine as one of raw materials, but the above patents are characterized in that: (1) The leaf surface resistance control agent has more preparation raw materials, complicated preparation process and special instruments, and is not suitable for popularization and use of common farmers. (2) The use of raw materials for preparing part of the leaf surface control agent has certain environmental risks, such as sodium selenite. (3) The leaf surface control agent containing L-cysteine is applied to reducing the cadmium content of rice grains, and the use method of the leaf surface control agent in the safe production of leaf vegetables is not clear. In summary, there is a need to design an environment-friendly foliar retarding and controlling agent for reducing the cadmium content of pakchoi by using L-cysteine and a use method thereof.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for using a leaf surface resistance and control agent of L-cysteine in planting of pakchoi, and the leaf surface resistance and control agent can effectively prevent and control cadmium after being sprayed on the leaves of the pakchoiThe fertilizer is transported to the edible parts of the pakchoi, and can effectively reduce medium and light cadmium-polluted soil (the cadmium content is less than or equal to 1.5 mgkg) -1 ) The cadmium content of the medium and small cabbages solves the problems mentioned in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a use method of a leaf surface resistance and control agent of L-cysteine in Chinese cabbage planting comprises the following steps:
step one, fully stirring and dissolving L-cysteine at a certain water temperature to prepare 10mmol L -1 、20mmol L -1 And 30mmol L -1 Collecting and storing three L-cysteine solutions with three concentrations to obtain the leaf surface resistance control agent;
step two, spraying a first foliage resistance and control agent after the Chinese cabbages grow to the three-leaf and one-heart;
and step three, spraying is also carried out on 7 th and 14 th days after the first spraying, and spraying is carried out for three times.
Preferably, in the first step, the certain water temperature is 25 ℃.
Preferably, in the second step, the first spraying of the foliage resistance and control agent refers to the spraying concentration of 30mmol L -1 The leaf surface controlling agent of (1).
Preferably, in the second step, the spraying is to spray water mist evenly distributed on the blades.
Preferably, in the spraying of the second step or the third step, the spraying parts are the surface and the back of the leaf.
Preferably, after the leaf surface inhibition and control agent adopting the L-cysteine solution is sprayed on the pakchoi, the transfer of cadmium from soil to the overground part can be well inhibited, and the transfer coefficient is as low as 0.61.
Preferably, 30mmol L is used -1 The L-cysteine with the concentration is sprayed in the three-leaf one-heart period of the pakchoi, so that the chlorophyll content in the pakchoi can be increased: in particular, the chlorophyll a in the pakchoi body reaches 1.211.75mgL -1 Chlorophyll b reaches 0.541.75mg L -1 The total chlorophyll content is up to 1.75mg L -1 。
The beneficial effects of the invention are: the invention discloses a methodThe method for spraying the leaf surface control agent can reduce the cadmium content on the upper part of the Chinese cabbage, can better inhibit the cadmium from transferring from the soil to the overground part, and has the transfer coefficient as low as 0.61; using 30mmol L -1 The L-cysteine with the concentration is sprayed in the three-leaf one-heart period of the pakchoi, so that the chlorophyll content in the pakchoi can be increased: chlorophyll a in the body of the pakchoi reaches 1.211.75mg L -1 Chlorophyll b reaches 0.541.75mgL -1 The total chlorophyll content is up to 1.75mg L -1 。
Drawings
FIG. 1 is a diagram illustrating the influence of spraying of a leaf surface control agent on the cadmium content of overground parts of pakchoi;
FIG. 2 is a graph showing the influence of spraying of a foliage resistance and control agent on the cadmium content in the underground part of the pakchoi;
FIG. 3 is a comparison graph of the transport coefficient of pakchoi to cadmium in the embodiment of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention provides a preparation method of a foliage resistance and control agent, wherein the main raw material of the preparation is L-cysteine, and the preparation method comprises the following steps:
(1) Three beakers were prepared, and 500ml of water was added, respectively, maintaining the water temperature at 25 ℃.
(2) 0.6058g, 1.2116g and 1.8174g of L-cysteine are respectively weighed and added into three beakers, and the L-cysteine is prepared into 10mmol L after being fully stirred and dissolved -1 、20mmol L -1 And 30mmol L -1 And respectively filling the L-cysteine solutions with three concentrations to obtain the finished product of the leaf surface resistance control agent.
The invention provides a use method for reducing the cadmium content of pakchoi in medium and light cadmium-polluted soil by using the preparation, which comprises the following steps:
(1) The preparation is used for spraying on the leaves of the Chinese cabbage.
(2) The preparation is sprayed in the following three periods of growth of the pakchoi.
Period (1): the growing three leaves of the Chinese cabbage are carried out in the first heart stage.
Period (2): the three leaves of the pakchoi are grown one heart later and 7 days later.
Period (3): the three leaves of pakchoi were grown one heart later and day 14.
(3) The key part of the spray of the preparation is that the front and back surfaces of the pakchoi leaves are sprayed, and the water mist is uniformly distributed on the leaves.
(4) The spraying amount per mu of land is 210L.
Example 2
The molar mass of L-cysteine is 121.16g mol -1 0.6058g, 1.2116g and 1.8174g (error not more than +/-0.0002 g) are respectively weighed and respectively added into 500ml (25 ℃) of water, evenly stirred and prepared into L with the concentration of 10mmol -1 、20mmol L -1 And 30mmol L -1 The L-cysteine solution of (2). The spraying concentration of the L-cysteine in the experiment is set as 10mmol L -1 、20mmol L -1 And 30mmol L -1 Three treatments, each treatment group is respectively provided with three groups of time spraying: the spraying is carried out once in the three-leaf one-heart period, 7 days after the three-leaf one-heart period and 14 days after the three-leaf one-heart period respectively. And setting a CK blank group, wherein other conditions of the blank group are the same as those of the test group, and only spraying clear water. The key part of spraying is young stem leaves of the pakchoi, the front and the back of the pakchoi are sprayed, water mist is guaranteed to be evenly distributed on the blades, the spraying amount of each pot is 10mL, a paper towel is laid on the surface of the exposed soil, and the influence of the spraying agent on the blades in a dripping mode on the test is prevented. Three cabbages in each pot were tested in triplicate. Harvesting 7 days after the last spraying. The specific design is shown in table 1.
Table 1 experimental set of design
Table2 Experimental design of this study
Results of the experiment
Influence of leaf surface control agent on cadmium content of leaf of Chinese cabbage
The influence on the cadmium content of the overground parts of the pakchoi is shown in figure 1 after the foliage resistance and control agent is sprayed at different concentrations at different times under the cadmium stress. Compared with CK, spraying the leaf surface control agent can reduce the cadmium content of the overground part of the pakchoi, and the earlier the spraying time is, the smaller the cadmium content of the leaves of the pakchoi is; the higher the spraying concentration is, the lower the cadmium content of the leaves of the pakchoi is. Wherein, 10mmol L of the pesticide is sprayed in the first heart period of three leaves -1 Compared with the foliage resistance control agent sprayed 7 days and 14 days after the first heart of the three leaves, the foliage resistance control agent reduces the cadmium content of the leaves by 2.46 percent and 34.53 percent respectively. Spraying 20mmolL to three leaves in one heart period -1 Compared with the foliage resistance control agent sprayed seven days and fourteen days after one-heart period of three leaves, the foliage resistance control agent reduces the cadmium content of the leaves by 28.27 percent and 77.67 percent respectively. Spraying 30mmol L of radix Trifolii Praeparata in one heart stage -1 Compared with the foliage retardant sprayed 7 days and 14 days after one heart period of three leaves, the foliage retardant reduces the cadmium content of the leaves by 5.96 percent and 40.65 percent respectively. Therefore, 30mmol L of the pesticide is sprayed in the three-leaf one-heart stage -1 The leaf surface control agent has the most obvious effect of reducing cadmium.
The influence of spraying the foliage resistance and control agent with different concentrations on the cadmium content in the underground parts of the pakchoi under the cadmium stress condition is shown in a figure 2. Compared with CK, 20 and 30mmol L are sprayed in the first heart period of three leaves and 7 days later -1 The concentration of the leaf surface resistance control agent increases the cadmium content of the roots of the pakchoi. And the foliage resistance and control agent sprayed 14 days after the first heart period of three leaves has no significant influence on the cadmium content of roots. The earlier the spraying and the higher the concentration of the sprayed leaf surface control agent are, the transfer of cadmium from the underground part to the overground part is effectively controlled, so that more cadmium is remained at the roots of the pakchoi. Meanwhile, the low-concentration leaf surface control agent sprayed in the same period shows lower cadmium content in the root, because the transfer of cadmium to the overground part cannot be effectively controlled under low concentration, the leaf has higher cadmium concentration and the root has lower cadmium content. On the whole, the spraying of the leaf surface control agent is early and maintains a higher concentration level, which is beneficial to preventing the cadmium from transferring to the edible part of the leaf of the Chinese cabbage
Cadmium-stressed cabbage organismThe transport coefficient is shown in FIG. 3. As can be seen from the figure, the cadmium transport capacity of the pakchoi can be reduced by spraying the foliage resistance and control agent, and the transport coefficient is reduced along with the increase of the spraying concentration of the foliage resistance and control agent. Under the same spraying concentration, the earlier the spraying is, the lower the transport coefficient of cadmium is. Spraying 30mmol L of the mixture in the first heart period of three leaves -1 When the foliar resistance and control agent is in a concentration, the transfer coefficient is minimum 0.61, which shows that the biological transfer of the cadmium in the pakchoi can be obviously reduced by spraying the foliar resistance and control agent with higher concentration on the leaf surface in the early growth stage of the pakchoi
The influence of spraying L-cysteine on chlorophyll a and chlorophyll b of pakchoi and the total content of chlorophyll is shown in Table 2.
TABLE 2 chlorophyll a, b and total amount of chlorophyll in pakchoi after spraying L-cysteine
Spraying 10mmol L -1 The L-cysteine solution shows an increasing trend in the total amount of chlorophyll ab and chlorophyll in the front, middle and rear spraying periods, and 20mmol L of L-cysteine solution is sprayed -1 The total amount of chlorophyll ab and chlorophyll in the L-cysteine solution shows a trend of rising first and then falling in the front, middle and rear three spraying periods, and 30mmol L of L is sprayed -1 The L-cysteine solution shows a decreasing trend in the total amount of chlorophyll ab and chlorophyll in the first, middle and last spraying periods. The fact that the chlorophyll content in the bodies of the pakchoi can be obviously improved by spraying the high-concentration L-cysteine in the early growth stage of the pakchoi is proved. In conclusion, 30mmol L of the pesticide is sprayed in the first heart stage of three leaves -1 The total chlorophyll content reaches a maximum value of 1.75mg L -1 To say 30mmol L of spray -1 The earlier the cadmium inhibition and control effect is more obvious, the growth of the pakchoi is more beneficial.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof.
Claims (7)
1. A use method of a leaf surface resistance and control agent of L-cysteine in Chinese cabbage planting is characterized by comprising the following steps:
step one, fully stirring and dissolving L-cysteine at a certain water temperature to prepare 10mmol L -1 、20mmol L -1 And 30mmol L -1 Collecting and storing three L-cysteine solutions with three concentrations to obtain the leaf surface resistance control agent;
step two, spraying a first foliage resistance and control agent after the Chinese cabbages grow to the three-leaf and one-heart;
and step three, spraying is also carried out on 7 th and 14 th days after the first spraying, and spraying is carried out for three times.
2. The use method of the leaf surface resistance control agent of L-cysteine in Chinese cabbage planting according to claim 1, characterized in that: in the first step, the temperature of the certain water is specifically 25 ℃.
3. The use method of the L-cysteine foliage retardant in Chinese cabbage planting according to claim 1, characterized in that: in the second step, the first spraying of the foliage resistance and control agent refers to that the spraying concentration is 30mmol L -1 The foliage-controlling agent of (1).
4. The use method of the L-cysteine foliage retardant in the planting of the pakchoi as claimed in claim 1 or 3, characterized in that: in the second step, the spraying is to spray water mist evenly distributed on the blades.
5. The use method of the L-cysteine foliage retardant in Chinese cabbage planting according to claim 1, characterized in that: in the spraying in the second step or the third step, the spraying parts are the surface and the back of the leaves.
6. The use method of the L-cysteine foliage resistance and control agent in the pakchoi planting according to any one of claims 1-5, characterized in that: after the leaf surface inhibition and control agent adopting the L-cysteine solution is sprayed on the pakchoi, the transfer of cadmium from soil to the overground part can be well inhibited, and the transfer coefficient is as low as 0.61.
7. The use method of the L-cysteine foliage retardant in Chinese cabbage planting according to claim 1, characterized in that: using 30mmol L -1 The L-cysteine with the concentration is sprayed in the three-leaf one-heart period of the pakchoi, so that the chlorophyll content in the pakchoi can be increased: specifically, the chlorophyll a in the bodies of the pakchoi reaches 1.211.75mg L -1 Chlorophyll b reaches 0.541.75mg L -1 The total chlorophyll content is up to 1.75mg L -1 。
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| CN102049410A (en) * | 2009-11-04 | 2011-05-11 | 中国科学院沈阳应用生态研究所 | Method for strengthening feverfew to repair cadimium contaminated soil |
| CN107896606A (en) * | 2017-12-24 | 2018-04-13 | 黑龙江八农垦大学 | A kind of amino acid application process for improving celery petiole Quantitative Determination of Apigenin |
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