CN103271030A - Complex hormone for inhibiting germination of wheat ears - Google Patents
Complex hormone for inhibiting germination of wheat ears Download PDFInfo
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- CN103271030A CN103271030A CN201310198574XA CN201310198574A CN103271030A CN 103271030 A CN103271030 A CN 103271030A CN 201310198574X A CN201310198574X A CN 201310198574XA CN 201310198574 A CN201310198574 A CN 201310198574A CN 103271030 A CN103271030 A CN 103271030A
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Abstract
The invention belongs to the technical field of agricultural biology, and in particular relates to a wheat ear germination inhibitor. The wheat ear germination inhibitor is a complex hormone formed by abscisic acid and salicylic acid, wherein abscisic acid is 0.70 to 1.40 g, salicylic acid is 0.69 to 1.38 g, tap water is 1 liter, and the pH value is 5.8 to 6.7. The complex hormone provided by the invention has the advantages of low service cost, simple and flexible method, obvious effects and no toxicity, improves pests and diseases resistance and stress resistance of plants, and can be used for the development of environment-friendly novel wheat germination inhibitors.
Description
Technical field
The invention belongs to agricultural biological technical field, be specifically related to a kind of wheat ear germinating inhibitor, the present invention is used for the wheat cultivation field and wheat is newly gathered in the crops seed, and major function is to suppress seed sprouting and fringe germination.
Background field
Wheat ear germinating refers to run into wet weather before the harvesting wheat or under the wet environment condition, seed is in the phenomenon of head sprouting.It is a kind of global climatic damage that fringe germinates.States such as Japan, Britain, Germany, the U.S., Canada, Australia all are subjected to fringe germination harm, and Canada and Australia are particularly serious.The middle and lower reach of Yangtze River area fringe germination problem of China is the most outstanding, and northeast spring wheat district, northwest spring wheat district, the Yellow River and Huai He River winter wheat district fringe germinate to endanger also to be and increase the weight of trend year by year in recent years.Wheat ear germinating causes a series of biochemical reaction of the inner generation of seed, particularly enzymic activitys such as some carbohydrate degrading enzymes (as α-Dian Fenmei), proteolytic enzyme raise, reserve substance in degraded embryo and the endosperm, cause biceps to descend, thereby greatly damage output, quality and the kind value of wheat.How to control the wheat aging time fringe and germinate, be the significant problem that solution is badly in need of in the research of China's Wheat Production and genetic breeding always.
Wheat ear germinating is genetic character, is again the physiological trait that regulated by Physiology and biochemistry factor and environment.The common wheat seed dormancy phase is short, and especially the white wheat kind liked of peasant is germinateed extremely sensitively to fringe, meets rain harvest time and causes that very easily fringe germinates.The variety source shortage of the anti-wheat ear germinating of natural world, good resistant gene scarcity, and be difficult to select suitable genetic components in the breeding process, cultivate the anti growing out kind by the genetic improvement method and make slow progress.Studies show that in a large number Seed Dormancy and sprouting are the coefficient results of hormone such as gibberellin (GA), abscisic acid (ABA) and ethene.GA and sprouting of the seed of ABA play an important role, but the GA breaking dormancy, induce sprouting, ABA then induces and promotes dormancy, inhibition seed to sprout, and antagonism is arranged between the two, and salicylic acid (SA) is sprouted for crop seeds such as corn, barley, wheats also inhibitory action.Yet, the reason that wheat ear germinating forms is comparatively complicated, King etc. discover between the content of fringe germination rate and endogenous ABA relevant not remarkable, Exogenous ABA can not effectively suppress the insensitive wheat seeds sprouting of ABA, report seed PPO activity, water absorption rates such as Wang Fengbao all are remarkable positive correlation with the fringe germination rate, be that PPO activity, water absorption rate are more low, ear germinating resistance is more strong.In addition, factors such as fringe portion shape, clever shell toughness and shape, kernel seed coat colour are all to fringe germination generation influence in various degree.
Present wheat breed anti growing out ability generally weak, fringe germinate and form under the machine-processed complicated situation, Chemical Control becomes another approach of control wheat ear germinating, and have easy, quick and effective advantage, exogenous chemical substances is regulated wheat seed hormonal balance or is changed the moisture of embryo, oxygen status etc., can reach the purpose that the control fringe germinates.Existing wheat ear germinating inhibitor mostly is growth inhibitor, hormone medicine.As use paclobutrazol and can effectively suppress the fringe germination, but consumption is big, cost is higher, effect is slow, have that cost is low, inhibition good with maleic hydrazide (Malaysia phthalein navel) though sprout inhibitor for the fringe of main component, bigger to the seed quality influence, and maleic hydrazide has carcinogenic doubting.In addition, the researcher also utilizes the inhibiting substances of extracting in forest tree seed, fruit or the branches and leaves to prevent and treat the wheat seed germination, has successively screened a series of natural mortifiers of N5, M3, YSR, RS and GP.Plant resource wheat ear germinating inhibitor can effectively suppress the growth of radicle and bud, the effect that the inhibition seed is sprouted is but not as hormone chemical classes (some no visible germination sign in seed surface, but inner reserve substance is degraded), though this class inhibitor is with low cost, but extraction step is many, process is long, can not make rapid response according to Changes in weather, and inhibition is subjected to the influence of extracting method big, active ingredient concentration is difficult to accurate control, there is fluctuation in the stability of field control effect, so do not apply in production as yet at present.
The present invention passes through the physiological mechanism of wheat ear germinating generation and the research of the factor of participation, act synergistically mutually on the manufacturing basis verifying ABA and SA, develop and significantly to suppress the hormone-type proprietary formula that wheat ear germinating is sprouted, reduced to wheat seed, can be used for production safety, nontoxic, non-harmful wheat ear germinating inhibitor, reach prolongation seed resting stage and suppress germinating capacity on the seed ear, to reduce the effect of fringe sprout damage.The present invention is environmentally friendly, simple effectively, effect is rapid, easy to use, has a wide range of applications.
Summary of the invention
The purpose of this invention is to provide a kind of fringe germination phenomenon that can effectively suppress in the wheat cultivation process, and safe, nontoxic, non-harmful wheat ear germinating inhibitor prescription.For realizing purpose of the present invention, wheat ear germinating inhibitor provided by the invention is filled a prescription, and is as follows in the compositing range of 1L solution ingredient and weight in grams thereof:
Abscisic acid 0.70～1.40
Salicylic acid 0.69～1.38
Running water 1L
The pH value of solution that is made into after the above-mentioned substance dissolving is between 5.8～6.7.
Best composed as follows in ingredient and weight in grams thereof in the 1L solution:
Abscisic acid 0.70
Salicylic acid 1.10
Running water 1L
The pH value of solution that is made into after the above-mentioned substance dissolving is between 5.8～6.7.
Abscisic acid in the component of the present invention is to participate in seed dormancy and the main plant hormone of sprouting regulation and control, also be that the multiple adversity gene of activated plant is expressed, strengthen the signal of interest molecule of plant opposing abiotic stress ability, successfully realize the commercial development of S-abscisic acid plant growth regulator Green Product.ABA mainly synthesizes to reduce seed α-Dian Fenmei and polyphenol oxidase (PPO) activity by suppressing gibberellin, reach the effect that suppresses seed germination, it is bigger that yet its germination inhibitor is influenced by wheat genotypes, the seed that the ABA of high concentration still can not effectively suppress the insensitive kind of ABA is sprouted, but can effectively suppress the growth of radicle and bud.
Salicylic acid in the component of the present invention (SA) is a kind of phenols hormone, and the gene expression of mediated plant pathogenesis-related proteins is to excite botanical system to obtain the key signal molecule of disease resistance, also is the important source material of medicine, agricultural chemicals, food and perfume industry.SA has the remarkable effect that suppresses wheat seed α-Dian Fenmei and PPO activity, and with variety and genetype do not obviously mutually, its enzymic activity suppresses degree and suppresses the seed effect of sprouting significantly to be better than ABA, but the growth of can not effectively suppress to sprout seed root and bud.
Effect and the mode of SA and ABA inhibition seed germination are variant, and the two is used in combination the remarkable inhibitory action of having strengthened seed α-Dian Fenmei and PPO activity, and it suppresses better effects if that seed is sprouted and fringe germinates.SA and ABA synergy can suppress wheat ear germinating effectively, and effective working concentration of each component is lower than the concentration of one-component, has reduced use cost.Component of the present invention has and improves the effect that ability is coerced in plant disease-resistant insect pest and degeneration-resistant border, and usage simple and flexible, effect are remarkable, nontoxic, can be used for the product development of the novel wheat ear germinating inhibitor of environmental friendliness, are applicable to that the land for growing field crops of China's wheat produces.
Description of drawings
Fig. 1 is used for the comparison diagram of different genotype wheat seeds sprouting inhibition for the present invention.Abscissa is that hormones of different concentrations (combination) is handled, and ordinate is percentage of seedgermination.
Show wheat line CK4-7;
Show wheat line CK4-18;
Show wheat breed K199;
Show wheat breed interior 8;
Show wheat line IK3-1-23.
Embodiment
Illustrate below, but the present invention is not limited to following example.
Embodiment 1
Test material: be test material with easy fringe germinated wheat kind (being) K199, interior 8, IK3-1-23, CK4-7, CK4-18.Wherein K199, interior 8, IK3-1-23 are the insensitive material of ABA, and CK4-7 and CK4-18 are the ABA sensitive material.
The preparation of composite hormone of the present invention and use: take by weighing an amount of abscisic acid or salicylic acid and directly be dissolved in running water to the use final concentration (seeing the following form 1) that needs.The ripe dry seeds of wheat is carried out surface sterilization handle (75% alcohol disinfecting 1min, 10% clorox 10min, distilled water cleans 4 times), place the culture dish that is lined with double-deck filter paper then, and respective handling liquid/ware of adding 10ml, sprout experiment under 26 ℃ of constant temperature, dark condition, contrast and be distilled water, test repeats 3 times.
Result of use of the present invention: plant skin with embryo portion and break and be the germination standard of sprouting, sprout rate and relative inhibition of statistics after six days.The result shows (table 1, Fig. 1), composite hormone of the present invention can significantly, stable suppress wheat seeds sprouting, obviously is better than the effect of one-component, relative inhibition to CK4-7 and K199 reaches 100% and 99.78% respectively, and gibberellin (GA) does not have obvious antagonism to its inhibition.Aqua of the present invention also has remarkable inhibition to the wheat different genotype.
The different HORMONE TREATMENT of table 1 are to the influence of wheat seeds sprouting
Remarks: 0.05 significance detection level.
Embodiment 2
Test material: 8 is test material in the ABA responsive type wheat breed that germinates with Yi Sui.
The preparation of composite hormone of the present invention and use: take by weighing an amount of abscisic acid or salicylic acid and directly be dissolved in running water to the use final concentration (seeing the following form 2) that needs.Choose the wheat head of 40 days (being ripening stage) maturity unanimities after the flowering, after different hormone solution spray processing (3 fringe/processing), insert and be equipped with in the Plastic Drum of water, and preserve moisture the experiment of in 26 ℃ of insulating boxs, germinateing with plastic sack along the bung covering.Test 3 repetitions, contrast and be distilled water.
Result of use of the present invention: plant skin with embryo portion and break and be the germination standard of sprouting, statistics head sprouting rate and relative inhibition after five days.The result shows (table 2), and composite hormone of the present invention can significantly, stable suppress wheat seed head sprouting rate, and relative inhibition reaches 90.57%, and obviously is better than the result of use of one-component.
8 head sprouting rate in table 2 wheat breed
Remarks: 0.05 significance detection level.
Claims (2)
1. composite hormone that suppresses wheat ear germinating is characterized in that: be dissolved in the running water by abscisic acid and salicylic acid and make, its amount ratio is abscisic acid 0.70～1.40 gram: salicylic acid 0.69～1.38 gram: 1 liter in running water; PH is 5.8～6.7.
2. the composite hormone of the described inhibition wheat ear germinating of claim 1 is characterized in that: described amount ratio is abscisic acid 0.70 gram: salicylic acid 1.10 grams: running water 1L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310198574XA CN103271030A (en) | 2013-05-24 | 2013-05-24 | Complex hormone for inhibiting germination of wheat ears |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135271A (en) * | 2016-07-04 | 2016-11-23 | 中国农业科学院油料作物研究所 | A kind of Semen Brassicae campestris vivipary inhibitor and application thereof |
| CN111758727A (en) * | 2020-06-15 | 2020-10-13 | 江苏徐淮地区淮阴农业科学研究所 | Germination inhibitor and preparation method and application thereof |
| CN114642206A (en) * | 2022-03-29 | 2022-06-21 | 贵州省烟草公司贵阳市公司 | Tobacco bud inhibitor suspending agent, preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318300A (en) * | 2001-04-25 | 2001-10-24 | 四川龙蟒福生科技有限责任公司 | Plant growth regulating method using dormin |
| CN1377581A (en) * | 2002-04-23 | 2002-11-06 | 四川龙蟒福生科技有限责任公司 | Plant growth regulating composition capable of preventing seed ear from sprouting |
| CN101773137A (en) * | 2010-01-26 | 2010-07-14 | 湖南农业大学 | Hybrid rice pre-harvest sprouting inhibitor |
-
2013
- 2013-05-24 CN CN201310198574XA patent/CN103271030A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318300A (en) * | 2001-04-25 | 2001-10-24 | 四川龙蟒福生科技有限责任公司 | Plant growth regulating method using dormin |
| CN1377581A (en) * | 2002-04-23 | 2002-11-06 | 四川龙蟒福生科技有限责任公司 | Plant growth regulating composition capable of preventing seed ear from sprouting |
| CN101773137A (en) * | 2010-01-26 | 2010-07-14 | 湖南农业大学 | Hybrid rice pre-harvest sprouting inhibitor |
Non-Patent Citations (1)
| Title |
|---|
| 熊愈辉: "发芽抑制物质研究综述", 《湖州师范学院学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135271A (en) * | 2016-07-04 | 2016-11-23 | 中国农业科学院油料作物研究所 | A kind of Semen Brassicae campestris vivipary inhibitor and application thereof |
| CN106135271B (en) * | 2016-07-04 | 2018-10-30 | 中国农业科学院油料作物研究所 | A kind of rape seed vivipary inhibitor and its application |
| CN111758727A (en) * | 2020-06-15 | 2020-10-13 | 江苏徐淮地区淮阴农业科学研究所 | Germination inhibitor and preparation method and application thereof |
| CN111758727B (en) * | 2020-06-15 | 2021-06-04 | 江苏徐淮地区淮阴农业科学研究所 | Germination inhibitor and preparation method and application thereof |
| CN114642206A (en) * | 2022-03-29 | 2022-06-21 | 贵州省烟草公司贵阳市公司 | Tobacco bud inhibitor suspending agent, preparation method and application thereof |
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Application publication date: 20130904 |