CN111758727A - Germination inhibitor and preparation method and application thereof - Google Patents
Germination inhibitor and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
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- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The invention belongs to the technical field of inhibitors, and particularly relates to a germination inhibitor and a preparation method and application thereof. The invention provides a germination inhibitor which comprises the following components in parts by mass: 0.2-0.8 part of chlormequat chloride, 34-64 parts of fulvic acid, 3-18 parts of B vitamins, 42-82 parts of monopotassium phosphate and 8-20 parts of surfactant. The germination inhibitor provided by the invention can inhibit the germination of the rice ears, thereby improving the quality of rice and the quality of seeds.
Description
Technical Field
The invention belongs to the technical field of inhibitors, and particularly relates to a germination inhibitor and a preparation method and application thereof.
Background
The rice is used as a main grain crop in China, the sowing area accounts for about 30% of the sowing area of the crop, and the yield is more than 40% of the total grain yield. In recent years, with frequent extreme weather caused by warming of climate, rice often encounters continuous high-temperature rainy days from grain filling to mature period, so that germination of seed ears is commonly caused. The germination rate of the ear in normal years is usually 5-10%, and the germination rate in special years (in high-temperature rainy days) can reach 60-80%. The main symptoms of the ear germination are that the ear germination is green, the viability of ungerminated grains is reduced, the quality of rice and the quality of seeds are seriously influenced, and great loss is caused to seed production enterprises.
At present, the pre-harvest sprouting inhibitors at home and abroad are mainly growth retardation substances and chemical inhibitors, but still have the problems of poor inhibition effect and serious yield reduction. And the chemical bud inhibitor has a good bud inhibiting effect, but has mutagenic and carcinogenic effects on animals and plants, and has serious environmental pollution.
Disclosure of Invention
In view of the above, the present invention aims to provide a germination inhibitor, a preparation method and an application thereof, the germination inhibitor provided by the present invention can inhibit the germination of rice ears, so as to improve the quality of rice and the quality of seeds, and the germination inhibitor provided by the present invention adopts natural components, does not add chemical agents, and has no pollution to the environment.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a germination inhibitor which comprises the following components in parts by mass: 0.2-0.8 part of chlormequat chloride, 34-64 parts of fulvic acid, 3-18 parts of B vitamins, 42-82 parts of monopotassium phosphate and 8-20 parts of surfactant.
Preferably, the B vitamins are one or a combination of more of thiamine, riboflavin and pyridoxine; when the B vitamins are a mixture of thiamine and riboflavin, the mass ratio of the thiamine to the riboflavin is 6-4: 4-2; the surfactant is Tween 20 or Tween 80.
Preferably, the germination inhibitor is an aqueous solvent; the dosage of water in the germination inhibitor is calculated by the concentration of chlormequat chloride of 0.2-0.8 g/L.
The invention provides a preparation method of the germination inhibitor in the technical scheme, which comprises the following steps: mixing the above components to obtain the germination inhibitor.
Preferably, when the germination inhibitor is an aqueous solvent, the preparation method comprises the steps of:
(1) mixing fulvic acid, potassium dihydrogen phosphate and water to obtain a primary inhibitor;
(2) and mixing the primary inhibitor with B vitamins, chlormequat chloride and surface activity to obtain the germination inhibitor.
The invention provides an application of the germination inhibitor in the technical scheme or the germination inhibitor prepared by the preparation method in rice ears.
Preferably, the application comprises spraying 30-60 days after the rice flowers.
Preferably, the spraying is performed on non-rainy days.
Preferably, the germination inhibitor is applied after dilution; the dilution mode is water dilution; the mass ratio of the volume of the germination inhibitor to water is 100-150 mL: 15-30 kg.
Preferably, the spraying is carried out on a rice ear layer and a leaf surface layer.
The invention provides a germination inhibitor which comprises the following components in parts by mass: 0.2-0.8 part of chlormequat chloride, 34-64 parts of fulvic acid, 3-18 parts of B vitamins, 42-82 parts of monopotassium phosphate and 8-20 parts of surfactant. In the invention, chlormequat chloride can inhibit the synthesis of endogenous gibberellin in grains and reduce the content of gibberellin; simultaneously inhibiting the activity of endogenous alpha-amylase of grains; the fulvic acid can promote photosynthesis of rice, improve grain filling and increase starch accumulation, and can improve stress resistance of rice when being mixed with chlormequat chloride and has the effect of inhibiting seed germination; the B vitamins participate in a plurality of cell physiological metabolic processes, such as generation of acetyl coenzyme A, TCA cycle, pentose phosphate pathway, Calvin cycle and the like, can improve stimulation of the cells to stress factors, enhance stress resistance of plants, achieve the effect of promoting plant growth and improve yield; the monopotassium phosphate can promote grain filling in the mature period, improve the seed setting rate and thousand seed weight of rice and enhance the stress resistance and disease resistance of the rice. The surfactant can promote the attachment and permeation of the liquid medicine on the surface of the plant leaf, increase the absorption of the liquid medicine and further effectively enhance the effect of the medicament. The invention mixes chlormequat chloride, fulvic acid, B vitamins, potassium dihydrogen phosphate and surfactant for use, achieves the technical effect of synergy, further inhibits the germination of rice ears, and further improves the technical effects of rice quality and seed quality.
In addition, the invention also provides a preparation method of the germination inhibitor, which is simple and feasible and can be used for household or industrial production.
Detailed Description
The invention provides a germination inhibitor which comprises the following components in parts by mass: 0.2-0.8 part of chlormequat chloride, 34-64 parts of fulvic acid, 3-18 parts of B vitamins, 42-82 parts of monopotassium phosphate and 8-20 parts of surfactant.
The components used in the present invention may be any commercially available products that are conventional in the art, unless otherwise specified.
The components of the germination inhibitor provided by the invention comprise 0.2-0.8 part of chlormequat chloride by mass, and preferably 0.4-0.6 part of chlormequat chloride by mass. In the invention, chlormequat chloride can inhibit synthesis of endogenous gibberellin in grains, reduce the content of gibberellin and simultaneously achieve the effect of inhibiting the activity of endogenous alpha-amylase in the grains.
The component of the germination inhibitor provided by the invention comprises 34-64 parts of fulvic acid, preferably 40-60 parts of chlormequat chloride by mass. In the invention, the fulvic acid can promote photosynthesis of rice, improve grain filling and increase starch accumulation, and can improve stress resistance of rice when being mixed with chlormequat chloride and has the function of inhibiting seed germination.
The component of the chlormequat chloride provided by the invention comprises 3-18 parts of B vitamins by mass, preferably 10-14 parts. In the invention, the B vitamins are preferably one or a combination of thiamine, riboflavin and pyridoxine; when the B vitamins are thiamine and riboflavin, the mass ratio of the thiamine to the riboflavin is preferably 6-4: 4-2, preferably 5: 3. In the invention, the B vitamins play a role of a catalyst, the effects of chlormequat chloride, fulvic acid and potassium dihydrogen phosphate are improved, and the synergistic effect of several substances is ensured; meanwhile, the B vitamins can promote the grouting of rice, convert substances in grains and convert saccharides into starch for storage, so that the starch accumulation of rice grains is facilitated, the grain weight is increased, and the technical effect of increasing the crop yield is ensured.
The component of the chlormequat chloride germination inhibitor comprises 42-82 parts of monopotassium phosphate by mass, preferably 50-70 parts of monopotassium phosphate by mass. In the invention, the monopotassium phosphate can promote grain filling in the mature period, and the effects of fulvic acid and B vitamins are combined, so that the effects of improving the maturing rate and thousand seed weight of rice are achieved, and the technical effect of increasing the plant yield is achieved.
The chlormequat chloride germination inhibitor comprises 8-20 parts of surfactant, preferably 10-15 parts by mass. In the present invention, the surfactant is preferably tween 20 or tween 80. The invention further promotes the adhesion and the penetration of the germination inhibitor on the surface of the plant leaf by using the surfactant as Tween series, increases the absorption of liquid medicine and effectively enhances the action effect of the medicament.
In the present invention, the germination inhibitor is preferably an aqueous solvent; the amount of water used in the germination inhibitor is preferably 0.2 to 0.8g/L, and more preferably 0.4 to 0.6g/L, based on the concentration of chlormequat chloride.
In the invention, chlormequat chloride can inhibit synthesis of endogenous gibberellin in grains, reduce the content of gibberellin, achieve the activity of inhibiting endogenous alpha-amylase in the grains, and simultaneously, when the chlormequat chloride is used in combination with fulvic acid, the photosynthesis of rice can be promoted, grain grouting can be improved, the accumulation of starch can be increased, the stress resistance of the rice can be improved, and the function of inhibiting seed germination can be achieved; under the action of a B-group vitamin catalyst, chlormequat chloride, fulvic acid and potassium dihydrogen phosphate improve the synergistic effect of the components, and meanwhile, the B-group vitamin can promote the grouting of rice and the substance conversion in grains, converts sugar into starch for storage, is beneficial to the starch accumulation of rice grains, increases the grain weight and ensures that the technical effect of increasing the crop yield is achieved; the efficacy of the potassium dihydrogen phosphate is combined with the efficacy of the fulvic acid and the B vitamins, so that the grain filling in the mature period can be promoted, the effects of improving the maturing rate and the thousand kernel weight of the rice are achieved, and the technical effect of increasing the plant yield is achieved. The invention mixes chlormequat chloride, fulvic acid, B vitamins, potassium dihydrogen phosphate and surfactant for use, promotes the adhesion and permeation of a germination inhibitor on the surface of plant leaves, increases the absorption of liquid medicine, effectively enhances the action effect of the medicament, ensures the technical effect of synergistic interaction, further inhibits the emergence of the germination phenomenon of rice ears, and further improves the technical effects of the quality of rice and the quality of seeds.
In addition, the germination inhibitor provided by the invention adopts natural components, does not contain chemical reagents, and has no pollution to the environment.
The invention provides a preparation method of the germination inhibitor in the technical scheme, which comprises the following steps: mixing the above components to obtain the germination inhibitor.
When the germination inhibitor is an aqueous solvent, the method for preparing the germination inhibitor preferably comprises the steps of:
(1) mixing fulvic acid, potassium dihydrogen phosphate and water to obtain a primary inhibitor;
(2) and mixing the primary inhibitor with B vitamins, chlormequat chloride and surface activity to obtain the germination inhibitor.
The invention mixes fulvic acid, potassium dihydrogen phosphate and water to obtain the primary inhibitor. The stirring method of the present invention is not limited at all, and the method may be a method known to those skilled in the art. According to the invention, fulvic acid and potassium dihydrogen phosphate are dissolved firstly, so that a slightly acidic environment can be provided for B vitamins, the stability of the germination inhibitor is improved, the effect of inhibiting the germination of rice ears is exerted to the greatest extent, and the quality of rice and the quality of seeds are improved.
After the primary inhibitor is obtained, the primary inhibitor is mixed with B vitamins, chlormequat chloride and surface activity to obtain the germination inhibitor. In the present invention, the temperature for mixing in the step (2) is preferably normal temperature. The present invention is not limited to any mixing method, and may be achieved by means of techniques known to those skilled in the art.
The invention also provides the application of the germination inhibitor in the technical scheme or the germination inhibitor prepared by the preparation method in the technical scheme on rice ears. In the present invention, the use is preferably the use of the germination inhibitor for inhibiting the germination of rice ears, improving the quality of rice and the quality of seeds. In the invention, the application preferably comprises spraying on the 30 th-60 th day after the rice blooms, and more preferably on the 30 th day; the spraying is preferably carried out in non-rainy days, and further preferably carried out in sunny days; the germination inhibitor is preferably applied after dilution; the dilution mode is preferably water dilution; the mass ratio of the volume of the germination inhibitor to water is preferably 100-150 mL: 15-30 kg, more preferably 18-26 kg of 100 mL; the spraying is preferably carried out on the rice ear layer and the leaf surface layer.
In order to further illustrate the present invention, a germination inhibitor, a method for preparing the same, and applications thereof, which are provided by the present invention, will be described in detail with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparing materials according to mass portion: 0.2 part of chlormequat chloride, 34 parts of fulvic acid, 3 parts of B vitamins, 42 parts of monopotassium phosphate and 8 parts of surfactant, wherein the mass ratio of thiamine to riboflavin is 6: 4.
weighing 34g of fulvic acid and 42g of monopotassium phosphate, adding 500mL of water, and uniformly stirring and dissolving; then adding 1.8g of thiamine and 1.2g of riboflavin, simultaneously adding 200mL of water, and stirring to dissolve uniformly; then 0.2 g of chlormequat chloride and 8g of Tween 20 are added and stirred uniformly; and finally adding water to a constant volume of 1000mL, and fully and uniformly stirring to obtain the inhibitor. 1L of the solution was dispensed into 10 bottles (100 mL each).
Example 2
Preparing materials according to mass portion: 0.5 part of chlormequat chloride, 48 parts of fulvic acid, 12 parts of B vitamins, 62 parts of monopotassium phosphate and 13 parts of surfactant, wherein the mass ratio of thiamine to riboflavin is 5: 3.
weighing 48g of fulvic acid and 62g of monopotassium phosphate, adding 600mL of water, and uniformly stirring and dissolving; then adding 7.5g of thiamine and 4.5g of riboflavin, simultaneously adding 200mL of water, and stirring to dissolve uniformly; then 0.5 g of chlormequat chloride and 13g of Tween 20 are added and stirred uniformly; and finally adding water to a constant volume of 1000mL, and fully and uniformly stirring to obtain the inhibitor. 1L of the solution was dispensed into 10 bottles (100 mL each).
Example 3
Preparing materials according to mass portion: 0.8 part of chlormequat chloride, 64 parts of fulvic acid, 18 parts of B vitamins, 82 parts of potassium dihydrogen phosphate and 20 parts of surfactant, wherein the mass ratio of thiamine to riboflavin is 4: 2.
weighing 64g of fulvic acid and 82g of monopotassium phosphate, adding 700mL of water, and uniformly stirring and dissolving; then adding 12g of thiamine and 6g of riboflavin, simultaneously adding 200mL of water, and stirring to dissolve uniformly; then 0.8g of chlormequat chloride and 20g of Tween 20 are added and stirred uniformly; and finally adding water to a constant volume of 1000mL, and fully and uniformly stirring to obtain the inhibitor. 1L of the solution was dispensed into 10 bottles (100 mL each).
Example 4
Thiamine was absent in the same manner as in example 1.
Example 5
Riboflavin was absent in the same manner as in example 1.
Comparative example 1
The chlormequat chloride fraction was absent in the same manner as in example 1.
Comparative example 2
In the same manner as in example 1, the B vitamin component was absent.
Comparative example 3
In the same manner as in example 1, the fulvic acid component was absent.
Comparative example 4
In the same manner as in example 1, the monopotassium phosphate component was absent.
Comparative example 5 (absence of surfactant component)
The surfactant component was absent in the same manner as in example 1.
Comparative example 6
Only chlormequat chloride was contained in the same manner as in example 1.
Comparative example 7
Only the B vitamin component was included in the same manner as in example 1.
Comparative example 8
It was prepared in the same manner as in example 1, containing only fulvic acid.
Comparative example 9
In the same manner as in example 1, only the potassium dihydrogen phosphate component was present.
Comparative example 10
Only surfactant was included in the same manner as in example 1.
Application example
The germination inhibitors prepared in the blank control, examples 1 to 3 and comparative examples 1 to 10 were subjected to field experiments, and some indexes were detected as follows:
design of experiments
Performing pesticide effect tests in 2019 in the modern agricultural science and technology park of Huaiyin agricultural science research institute of Xuhuai region of Jiangsu province;
the test varieties are: the Nanjing 9108 and the II you 128 are sown in 2019 in 5 months and 10 days;
the germination inhibitors prepared in examples 1 to 3 and comparative examples 1 to 5 are sprayed in the middle and later stages of rice grain filling (9 months and 20 days), and diluted according to the proportion of 100ml of the inhibitor and 20kg of water, wherein the area of each treatment cell is 4 × 6m2And repeating the steps for 3 times, wherein the spraying dosage of each treatment cell is l000mL, and the spraying of equal amount of clear water is used as a control, so that the field management is performed normally.
Continuous medium to heavy rain weather appears in the test area from 10 months to 5 days to 7 days in 2019. The rice is harvested at 28 days in 10 months, the germination rate of the ears of the plot is sampled and investigated 1 day before harvesting, and the thousand kernel weight of grains, the activity of alpha-amylase and other indexes of the plots are investigated after harvesting. The formula for calculating the inhibition and synergy ratio of the components and the coordination effect among the components to the sprouting of the panicle is as follows: the synergistic ratio is the single-agent effect/(single-agent + synergistic agent) effect.
When the synergistic ratio of the inhibitor provided in example 1 is calculated, the data of example 1 is taken as the synergist, the data of the control group is taken as the single-agent effect, and the (single-agent + synergist) effect refers to the experimental data of a new component obtained after the synergist is added into the single-agent (in the invention, the single-agent is taken as the control group, namely clear water, the new component obtained after all the reagents in example 1 are added is taken as example 1, and the effect of the clear water is 0 when the clear water and the example 1 are combined, so the effect of the single-agent + synergist in the invention is taken as the effect in example 1); when the synergistic ratio of the inhibitors provided by the embodiments 2 to 5 is calculated, the method is equivalent to the synergistic ratio solving method of the inhibitor provided by the embodiment 1, and the single-dose effects are data of a control group, and the (single-dose + synergistic agent) effects respectively correspond to the effects of the embodiments 2 to 5; when calculating the potentiating ratio of the inhibitor provided in comparative example 1, the data of comparative example 1 is taken as the potentiator and the data of comparative example 6 is taken as the single-dose effect, and the (single-dose + potentiator) effect refers to the experimental data of the new component obtained by adding the component of comparative example 1 to the component of comparative example 6, i.e., the data of example 1; when the synergistic ratio of the inhibitor provided by the comparative examples 2-5 is calculated, the method is equivalent to the solving mode of the synergistic ratio of the inhibitor in the comparative example 1, the single-dose effect corresponds to the data of the comparative examples 7-10 respectively, and the (single-dose + synergist) effect corresponds to the data of the example 1; when calculating the synergy ratio of comparative example 6, using comparative example 6 as the synergist and the data of comparative example 1 as the single-dose effect, and the (single-dose + synergist) effect refers to the experimental data of the new composition obtained by adding the component of comparative example 6 to the component of comparative example 1, i.e. the data of example 1; when the synergistic ratio of the inhibitors provided in the comparative examples 7 to 10 is calculated, the calculation is equivalent to the solution mode of the synergistic ratio of the inhibitor in the comparative example 6, and the single-dose effects respectively correspond to the data of the comparative examples 2 to 5, and the (single-dose + synergist) effects respectively correspond to the data of the example 1. The results are shown in tables 1 and 2:
TABLE 1 influence of Rice ear Germination inhibitors on Rice ear Germination Rate and thousand Kernel weight in the mature period
As can be seen from the test data recorded in Table 1, the spraying of the rice pre-harvest sprouting inhibitor can obviously inhibit the pre-harvest sprouting of the rice in the mature period, the pre-harvest sprouting rates of the Nanjing 9108 and the IIyou 128 are both obviously reduced, the pre-harvest sprouting rates of the 5 cases of the Nanjing 9108 are respectively reduced by 76.4%, 79.6%, 73.9%, 72.0% and 73.2% compared with the control, and the pre-harvest sprouting inhibition synergistic ratio is 4.2, 4.9, 3.8, 3.6 and 3.7; the germination percentage on the ear of II excellent 128 is respectively reduced by 78.0%, 76.3%, 66.7%, 74.2% and 75.8% compared with the control, and the ratio of the inhibition and the synergy of the germination of the ear of grain is 4.5, 4.2, 3.0, 3.9 and 4.1. Meanwhile, the thousand grain weight of Nanjing 9108 is respectively increased by 10.9%, 12.0%, 10.2%, 10.9% and 9.8% compared with the control; the thousand grain weight of IIYOU 128 is respectively increased by 13.4 percent, 15.4 percent, 12.6 percent, 11.9 percent and 13.0 percent compared with the control. Therefore, the rice ear sprouting inhibitor can obviously inhibit the ear sprouting of rice, can promote the weight increase of seeds, and has obvious economic benefit. Comparing the experimental data of examples 1-5 with those of comparative examples 1-10, it can be seen that the ratio of the inhibition effect on the sprouting of the ears of the two rice varieties treated in comparative examples 1-10 is lower than that in examples 1-5 when any one of the components of examples 1-5 is absent or only one of the components is present; the average thousand-particle weight is less synergistic than that of the control, and especially, the effect of each single agent in the comparative examples 6 to 10 is obviously reduced compared with that of the examples 1 to 5. This indicates that the components of examples 1 to 5 have mutually coordinated and mutually promoted effects in the processes of the pre-harvest sprouting inhibitor and the formation of the thousand-kernel weight.
TABLE 2 influence of Rice ear Germination inhibitors on alpha-amylase activity and starch content of seeds at maturity
From the test data recorded in table 2, it can be seen that spraying the rice pre-harvest sprouting inhibitor can significantly inhibit the alpha-amylase activity in seeds of two rice varieties in the mature period, and increase the starch content of the seeds. The five example treatments significantly inhibited alpha amylase activity in southern japonica 9108 by 13.9%, 17.1%, 8.6%, 7.3% and 8.2%, respectively, compared to the control; the activity of the alpha amylase in the IIYOU 128 grains is respectively reduced by 8.4%, 13.0%, 7.1%, 8.0% and 8.4% compared with that of a control. Meanwhile, the rice pre-harvest sprouting inhibitor increases the starch content in the seeds in the mature period, and the starch content in the Nanjing 9108 seeds is respectively increased by 10.1%, 11.3%, 9.8%, 9.9% and 9.8% compared with the control; the starch content in the IIYOU 128 grains is respectively increased by 9.8%, 11.9%, 9.4%, 9.2% and 9.3% compared with the control. Therefore, the rice ear germination inhibitor can reduce the activity of alpha amylase in seeds in a mature period, thereby obviously reducing the germination probability of the rice ears, promoting the filling of the seeds, improving the starch content in the seeds and being beneficial to the increase of yield and the improvement of quality. Comparing the experimental data of examples 1-5 with those of comparative examples 1-10, it can be seen that the alpha amylase activity of the two rice varieties treated in comparative examples 1-10 is lower than that of the control and lower than that of examples 1-5, regardless of the absence or presence of any component or only one component of examples 1-5; the starch content is increased compared with the control, but still lower than that of the example 1, and particularly, the effect of each single agent in the comparative examples 6 to 10 is obviously reduced compared with that of the examples 1 to 5 and the comparative examples 1 to 5. This indicates that the components of examples 1-5 have a synergistic interaction in reducing alpha amylase activity and accumulating starch in the kernel.
From the above examples, it is clear that the germination percentage of the germination inhibitor provided by the present invention is reduced by more than 66%, the thousand kernel weight is increased by more than 10%, the starch content is increased by more than 9%, and the alpha amylase activity is reduced by more than 7% compared with the control group. Therefore, the germination inhibitor provided by the invention can reduce the activity of alpha amylase in seeds in the mature period, thereby obviously reducing the germination probability of rice ears, obviously inhibiting the germination of the rice ears and promoting the increase of the weight of the seeds; meanwhile, grain grouting can be promoted, the starch content in grains can be increased, yield increase and quality improvement are facilitated, and remarkable economic benefits are achieved.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (10)
1. The germination inhibitor is characterized by comprising the following components in parts by mass: 0.2-0.8 part of chlormequat chloride, 34-64 parts of fulvic acid, 3-18 parts of B vitamins, 42-82 parts of monopotassium phosphate and 8-20 parts of surfactant.
2. The germination inhibitor according to claim 1, wherein the B vitamins are one or more of thiamine, riboflavin and pyridoxine; when the B vitamins are a mixture of thiamine and riboflavin, the mass ratio of the thiamine to the riboflavin is 6-4: 4-2;
the surfactant is Tween 20 or Tween 80.
3. The germination inhibitor according to claim 1 or 2, wherein the germination inhibitor is an aqueous solvent; the dosage of water in the germination inhibitor is calculated by the concentration of chlormequat chloride of 0.2-0.8 g/L.
4. The method for producing a germination inhibitor according to any one of claims 1 to 3, comprising: mixing the above components to obtain the germination inhibitor.
5. The method according to claim 4, wherein when the germination inhibitor is an aqueous solvent, the method comprises the steps of:
(1) mixing fulvic acid, potassium dihydrogen phosphate and water to obtain a primary inhibitor;
(2) and mixing the primary inhibitor with B vitamins, chlormequat chloride and surface activity to obtain the germination inhibitor.
6. Use of the germination inhibitor according to any one of claims 1 to 3 or the germination inhibitor prepared by the method according to any one of claims 4 to 5 for ears of rice.
7. The use according to claim 6, wherein the application comprises spraying on days 30-60 after flowering of rice.
8. Use according to claim 7, wherein the spraying is carried out on non-rainy days.
9. The use according to any one of claims 6 to 8, wherein the germination inhibitor is used by dilution; the dilution mode is water dilution; the mass ratio of the volume of the germination inhibitor to water is 100-150 mL: 15-30 kg.
10. The use according to any one of claims 7 to 8, wherein the spraying is carried out on a rice ear layer and a leaf surface layer.
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