CN108849984B - Wheat drought-tolerance stress-tolerance compound regulator and application thereof - Google Patents
Wheat drought-tolerance stress-tolerance compound regulator and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Abstract
The invention relates to a wheat drought-resistant stress-resistant composite regulator and application thereof, comprising a seed soaking inducer for wheat seed treatment and a drought-resistant stress-resistant regulator for wheat foliage spraying. The drought-resistant stress-resistant regulator B for foliar spraying comprises diluted inducer A solution, foliar nutrient components, auxiliary stress-resistant components C and foliar spraying auxiliary components. The product of the invention can obviously enhance the capability of wheat in resisting drought and other stresses such as low temperature and weak light, slow down the loss of the adverse environment to the quality and the yield of the wheat, and has good resisting effect on wheat in China, particularly the growth environments such as insufficient illumination caused by drought environment, sudden low temperature and haze in a northern production area.
Description
Technical Field
The invention belongs to the technical field of plant stress-resistant regulators, and particularly relates to a wheat drought-tolerant stress-resistant compound regulator which comprises a seed soaking inducer for seed treatment and a drought-tolerant stress-resistant regulator for foliage spraying.
Background
Wheat is used as a main grain crop in the world, is an important food and feed source, and is one of the main grain crops with the largest demand in China and wide application fields. The northern China is a main wheat production area, and due to poor climatic conditions during sowing, particularly in drought and water-deficient climates, the germination rate of wheat seeds is low and the seedling growth is weak, and the wheat head formation and the wheat filling are further influenced by drought in spring, so that the wheat yield is finally influenced. In particular, various natural factors such as drought, dry hot air, late spring cold and the like in northern wheat areas all influence the tillering, fertilization and grain filling processes of wheat; particularly, the drought and low temperature in the northwest are more obvious, and the development and growth of wheat are seriously influenced. Therefore, how to ensure that wheat seeds sowed in the northern dry land can have sufficient nutrition to promote the germination and seedling growth of the wheat seeds, enhance the capability of the wheat to resist drought and other adversity stresses, resist severe natural conditions, are key to the improvement of yield, and have important significance for improving the quality and stabilizing the yield of the wheat.
Generally speaking, the stress-resistant means in the prior art mainly comprises selection of resistant varieties, timed irrigation, foliar fertilization and the like, but when severe drought stress or low-temperature stress is encountered, including sunlight irradiation intensity caused by haze weather is insufficient and other adverse natural factors, the artificial means not only wastes a large amount of water resources, but also has limited effect. Despite the advent of plant growth regulators such as paclobutrazol, the use of large amounts of chemical agents results in field and crop residues that not only pollute the environment, but also reduce the quality of the wheat product.
Therefore, aiming at the growth characteristics and growth environment of wheat, a high-efficiency drought-tolerant stress-resistant regulator is developed, and the drought tolerance, cold tolerance, salt and alkali tolerance and other stress tolerance of wheat are improved; has important significance for improving the wheat yield and the product quality.
Betaine is one of osmoregulation substances without toxic and side effects in plants, has strong moisture absorption performance and molecular weight of 117.15, is mainly distributed in plant cytoplasm and chloroplast, and can be accumulated in plant cells in large quantity under drought stress. Researches show that the betaine is not only an osmotic adjusting substance, but also has an osmotic protection effect, can maintain the osmotic pressure of cells, protect the structure and the integrity of biological macromolecules, maintain the normal physiological function of the biological macromolecules, and further protect the cells and the biological macromolecules of plants under different stress conditions. In addition, the betaine can reduce the membrane lipid peroxidation degree and improve the water content of the root system of the barley seedling and the fresh weight of the seedling under the salt stress. Exogenous betaine can increase the content of soluble sugar and soluble protein in low-temperature stress corn leaves. The slow release agent matched with the betaine is chitosan which has certain moisture absorption and retention performance and also has the antibacterial and insecticidal effects. Chitosan as a natural high molecular substance is easily degraded by soil microorganisms, and short-chain degradation products of the chitosan have the function of regulating the growth of plants and are nontoxic and harmless to crops, people and livestock.
In recent years, plant fructan is also gradually used for drought tolerance and stress resistance, and has good application potential in the aspects of drought tolerance and stress resistance of plants, but the research on the application of plant fructan to wheat is not reported. 5-aminolevulinic acid (delta-ALA for short) is an essential substance for synthesizing chlorophyll by organisms. Can promote crops to synthesize chlorophyll under dark light and regulate the synthesis of chlorophyll; the stability of chlorophyll and a light capturing system II is improved; promoting photosynthesis, promoting pore enlargement and improving gas exchange rate; dark respiration is inhibited, and photosynthetic efficiency is improved. The fertilizer is sprayed on the leaf surface and enters the plant body through the leaves and the tender epidermis to promote the growth of the root system; also can enhance the drought resistance, cold resistance, disease resistance and saline-alkali resistance of the plants.
CN107155757 discloses a stress resistance enhancing reagent for fruit trees, a use method and an application thereof, which comprises organosilicon, betaine, ABA and the like, wherein organosilicon is used as a spreader and can increase the surface wetting of leaves, the stress resistance of the fruit trees is improved by the ABA, the photosynthesis of the leaves is enhanced, the carbohydrates of the leaves and the fruits are supplemented, and the accumulation of the sugar in the fruits is increased, so the growth effect of the fruit trees in a reverse state is improved.
CN105347979A (application No. 201510936537.3) discloses a preparation method of a seed soaking agent special for corn, which prepares a leaching solution by microwave-assisted secondary crushing, enzymolysis, double-seaweed active ingredient collaborative leaching and double-strain liquid co-fermentation, wherein the leaching solution is rich in biological active ingredients such as trehalose, natural organic matters, nitrogen, phosphorus, potassium, active strains and the like, and acts on the surface layer of seeds to improve the drought stress resistance of the corn seeds.
CN103535380A discloses a crop drought-resistant agent with safety and good drought-resistant effect and application thereof. The active ingredients of the compound amino acid premix comprise compound amino acid, betaine, fulvic acid, organic matters and proline; wherein, the compound amino acid accounts for 7-13 parts by weight, the betaine accounts for 1-3 parts by weight, the fulvic acid accounts for 3-7 parts by weight, the organic matter accounts for 1-3 parts by weight, and the proline accounts for 6-70% of the total mass of the active ingredients; simultaneously, also discloses a preparation method and application of the crop drought-resistant agent.
CN103548446 (application No. 201310518497.1) discloses a drought-resistant treatment method for kidney bean seeds. The method of the invention is to treat the bean seeds by a plasma seed treatment machine and then treat the bean seeds by a special drought-resistant coating agent. The processing current of the plasma seed processor is 0.5-1.5A, and the processing times are 1-3; the drought-resistant seed coating agent special for the kidney beans comprises the following components: comprises 1-2% of chitosan, 10-15% of betaine, 0.01-0.05% of brassinolide, 0.5-1% of bactericide, 0.4-0.6% of insecticide, 0.072-0.144% of acetic acid and the balance of water by weight percentage.
Although the application of the stress-resistant regulator appears in the prior art, the growth characteristics and natural environment of each plant are different, the applicability of the plants is poor, and the effect and the efficacy of the regulator are single and are rarely matched with leaf fertilizers for use. The leaves are important external root nutritive organs of plants, and the foliar nutrition means that the plants absorb and utilize various nutrients through the surfaces of the leaves. Foliar nutrient components are a general term for fertilizers that provide nutrients to crops through the leaves of the crops. The nutrient components of the leaf surface are nutrient elements applied to the surface of the leaves of crops, and the nutrient elements play a basic function through the absorption of the leaves. The addition of the foliar nutrient components can greatly improve the drought resistance of seeds and seedlings and can also enhance the stress resistance of other aspects of plants.
In addition, the foliar fertilization can supplement nutrient absorption deficiency caused by underdeveloped roots or drought and other external stresses in the seedling stage and the later growth stage in time, and plays roles in strengthening seedlings and increasing yield. Under the saline-alkali and drought environments, the nutrient absorption of roots is inhibited, and the leaf surface spraying effect is good. The nutrient elements which are lacked by the growth of the sprayed crops can correct or improve the nutrient deficiency of the crops in time, especially the trace elements, and have the advantages which are incomparable with the soil fertilization.
Therefore, there is a need for the development of new complex drought tolerant stress tolerant regulators containing foliar nutritional components for wheat production.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a novel wheat drought-resistant stress-resistant regulator, which comprises a seed soaking inducer solution for wheat seed treatment, a drought-resistant stress-resistant regulator solution for seedling foliage spraying, a preparation method and application thereof.
Specifically, the technical scheme of the invention is as follows:
a composite drought-resistant and stress-resistant regulator for wheat is composed of seed-soaking inducer A for treating wheat seeds and drought-resistant and stress-resistant regulator B for spraying it on wheat leaves.
Wherein, the seed soaking inducer A comprises the following components in percentage by weight:
3-5 wt% of betaine, 2-5 wt% of fructopolysaccharide, 1-3 wt% of chitosan, 0.01-0.03 wt% of brassinolide, 0.01-0.05 wt% of sodium nitroprusside, 0.05-0.1 wt% of fulvic acid, 0.01-0.03 wt% of titanium citrate and the balance of distilled water.
The drought-resistant stress-resistant regulator B for foliar spraying comprises the diluted inducer A solution, foliar nutrient components, auxiliary stress-resistant components C, foliar spraying auxiliary components and supplementary fructan.
In the invention, the drought-resistant stress-resistant regulator B is prepared by the following steps:
diluting an inducer A solution into a diluted solution 8-10 times by using deionized water, adding a pre-prepared foliage nutrient component and an auxiliary stress-resistant component C, fully dissolving to prepare a compound solution, uniformly stirring, adding a foliage spraying auxiliary component into the compound solution, supplementing fructopolysaccharides to the concentration of 3-5g/L, uniformly stirring, and metering the volume by using deionized water to obtain a drought-resistant stress-resistant regulator B solution.
Preferably, in the above steps, 100ml of inducer A solution is diluted into 0.5-0.8L solution by deionized water, the pre-prepared foliar nutrient component and the auxiliary stress-resistant component C are added, fully dissolved to prepare a compound solution, the mixture is uniformly stirred, the foliar spray auxiliary component is added into the compound solution, the fructan is supplemented to 3-5g/L, the mixture is uniformly stirred, and the deionized water is used for fixing the volume to 1L, so that the drought-resistant stress-resistant regulator B solution is prepared.
In the seed soaking inducer A, the fructan is plant fructan, is a polymer of D-fructofuranose, can be selected from linear fructan or branched fructan, and is preferably linear fructan.
Specifically, timothy grass sugar type fructan or linear inulin polysaccharide (extracted from Jerusalem artichoke tubers) is preferred, and may also be selected from artificially synthesized fructans, such as kestose, 1-kestose, 6-kestose, neokestose, and the like.
The chitosan of the invention has a deacetylation degree of more than 85 percent and is a commercially available product with medium and low viscosity.
Additionally, the inducer a may also comprise an optional pesticide component.
In the drought-resistant stress-resistant regulator B, the foliar nutrient components are selected from at least two of calcium phosphate, monopotassium phosphate and ammonium nitrate; the content of the leaf surface nutrient components is 1-10 g/L.
Preferably, the content of the foliar nutrient components is 1-5 g/L.
In the invention, the auxiliary stress-resistant component C comprises humic acid, glycine chelate, diethylaminoethanol caproate, sodium borate, alpha-sodium naphthaleneacetate, choline chloride, 5-aminolevulinic acid and other growth regulators.
In the invention, the auxiliary components for foliage spraying comprise glycolipid biosurfactant, sodium lignosulfonate, sorbitol, a small amount of organic solvent and a pH regulator.
In the auxiliary stress-resistant component C, the glycine chelate is selected from at least one of calcium glycinate and zinc glycinate; the content of glycine chelate in the regulator B solution is 0.5-2 g/L.
Wherein, the dosage of the humic acid is 500-1000 mg/L.
Furthermore, the dosage of the sodium borate is 10-50mg/L respectively.
Furthermore, the dosages of the choline chloride and the alpha-sodium naphthaleneacetate are respectively 0.1-0.5 g/L.
Further, the dosage of the 5-aminolevulinic acid is 0.5-2 g/L.
Further, the other growth regulator is selected from at least one of sodium nitrophenolate, n-triacontanol and prohexadione calcium; the content of the regulator B solution is 1-20mg/L, preferably 1-10 mg/L.
In the present invention, in the foliar spray adjuvant composition, the glycolipid biosurfactant is preferably selected from industrialized glycolipid biosurfactants, including but not limited to: sophorolipid, rhamnolipid, algal glycolipid, etc.
Furthermore, the dosage of the glycolipid biosurfactant is 3-10 g/L.
Further, the organic solvent is selected from ethanol or propanol, and the dosage is 5-20 mL/L.
Further, the pH regulator is preferably salicylic acid, and the dosage of the pH regulator is 50-100 mg/L.
Further, the dosage of the sodium lignosulphonate is 0.5-1 g/L.
Furthermore, the dosage of the sorbitol is 0.5-1 g/L.
All components of the formulations of the present invention are known products, and are commercially available or prepared according to known art in the art.
The method or the steps of the seed soaking inducer A for treating the wheat seeds are as follows:
s1: seed soaking pretreatment:
diluting the inducer A solution by 10-15 times with deionized water, adding 1-1.5 wt% glycolipid biosurfactant as a suspension auxiliary agent, uniformly stirring, and soaking seeds at 25-30 ℃ for 4-8h, wherein the weight ratio of the diluted inducer solution to the seeds is 1: 1-1.5;
s2: surface spraying treatment:
after the seed is subjected to surface ventilation drying treatment, uniformly spraying undiluted inducer A solution, performing surface spraying treatment on the seed, and quickly performing ventilation drying after the surface spraying treatment; wherein the weight ratio of the inducer A solution to the seeds is 1: 10-20, preferably 1: 15-20.
In another aspect of the invention, a method for using the drought-resistant stress-resistant regulator for wheat is provided, which comprises the steps of using a seed soaking inducer A for wheat seed treatment and using a drought-resistant stress-resistant regulator B solution for wheat leaf surface spraying treatment; wherein the wheat seed treatment comprises the steps of seed soaking pretreatment and seed surface spraying treatment.
Wherein, the wheat foliage spray treatment step is:
diluting the drought-resistant stress-resistant regulator B solution by using 100-300 times of deionized water to form a spraying solution, and uniformly spraying the spraying solution on the surfaces of the leaves of the wheat seedling plants; wherein, the spraying agent can be sprayed at the early stage, the middle stage and the late stage of the drought period, and can also be sprayed when facing other adverse conditions such as low temperature and weak light stress; besides the seedling stage, the spraying can also be carried out in the jointing stage and the filling stage of the wheat, and the spraying can be carried out for 2-5 times continuously or at intervals in each stage.
Preferably, the spraying amount of the leaf surfaces is 15-20 kg/mu.
In a third aspect of the invention, the use of the wheat drought-enduring and stress-resistant compound regulator for regulating the growth of wheat is provided.
In the present invention, references to "comprising" of composition encompass both open-ended "including," "comprising," and the like, as well as closed-ended "consisting of …," and the like, and the like.
The beneficial effects of the invention also include but are not limited to the following aspects:
1. the main components of plant fructan, betaine and the like in the compound preparation are environment-friendly and nontoxic, no field residue exists, the seed soaking agent and the leaf surface spraying agent can effectively promote seed germination, improve seedling vigor, relieve the damage degree of wheat plants under drought and other natural stress conditions, and regulate the growth and development of the plants, so that the quality and yield of the wheat are improved.
2. The composite preparation contains the foliar nutrient components, wherein the glycine trace element chelate has high absorption efficiency, and when the composite preparation is used together with a regulator, the nutrient components have strong adhesive force on the leaves due to the existence of the components such as a surfactant, a slow-release agent and the like, the nutrient components have long retention time on the surfaces of the leaves, and the nutrient loss is greatly reduced. On the premise of improving the stress resistance of crops, the utilization efficiency of the foliar nutrient components is improved, and the additional fertilization times are reduced.
3. The plant fructan has the dual functions of stress resistance and biocompatibility, increases the transmembrane permeability of plant cell membranes, is beneficial to wetting the surface by liquid, promotes absorption, remarkably improves the resistance of wheat to drought, low temperature, high temperature and weak light stress, and has no field residue. The fructan combined with the surfactant can reduce the surface tension of the solution drops on the leaf surface, increase the adhesion of the leaf surface, prolong the detention time and promote the absorption of nutrients on the leaf surface; in addition, chitosan and levan also have excellent sterilization inhibiting performance and enhance the disease resistance of plant crops.
4. The foliar nutrient component, the stress-resistant regulator and the auxiliary component in the compound preparation provided by the invention are synergistic, so that the effects of nutrition supplementation, stress resistance and lasting and efficient absorption after foliar spraying are achieved; the foliar fertilizer is supplemented, simultaneously, the germination rate of wheat seeds and the drought resistance of seedlings and the comprehensive stress resistance of photosynthesis and the like under external stress such as low temperature, weak light and the like are greatly improved, and the normal growth and development of plants are ensured.
5. The method for preparing the regulator and the using method are simple and easy to implement, do not need complex equipment and flow, and are convenient for large-scale popularization and application.
6. The compound components of the invention complement and coordinate the functions of the components, can organically combine the nutritional requirement of seedlings with drought resistance and other stress resistance, not only enhances the adaptability of crops to drought and other stress environments, but also avoids the defects of unstable effect, short duration and low absorption efficiency when the components are applied independently.
In addition, the preparation method is simple, is suitable for large-scale production, and has good application prospect and market value.
Detailed Description
The present invention is described in detail below with reference to specific preparation examples and examples, but the use and purpose of these exemplary embodiments are merely to illustrate the present invention, and do not constitute any limitation to the actual scope of the present invention in any form, and the scope of the present invention is not limited thereto.
The following detailed description of preferred embodiments of the invention and the examples included therein will make it easier to understand the context of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.
The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
"consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
Example 1
A composite drought-resistant and stress-resistant regulator for wheat is composed of seed-soaking inducer A for treating wheat seeds and drought-resistant and stress-resistant regulator B for spraying it on wheat leaves.
The seed soaking inducer A comprises the following components in percentage by weight: 3 wt% of betaine, 3 wt% of timothy grass sugar type fructan, 1 wt% of chitosan, 0.01 wt% of brassinolide, 0.01 wt% of sodium nitroprusside, 0.05 wt% of fulvic acid, 0.01 wt% of titanium citrate and the balance of distilled water, and the volume is adjusted to 100 ml.
The drought-resistant stress-resistant regulator B is prepared by the following steps:
diluting 100ml of the inducer A solution into 0.5L of solution by using deionized water, adding a proper amount of leaf surface nutrient components and auxiliary stress-resistant components C, fully dissolving to prepare a compound solution, uniformly stirring, adding leaf surface spraying auxiliary components into the compound solution, supplementing the ladder grass sugar type fructan to 3g/L, uniformly stirring, and fixing the volume of the deionized water to 1L, thereby preparing the drought-resistant stress-resistant regulator B solution, wherein the leaf surface nutrient components, the auxiliary stress-resistant components C and the leaf surface spraying auxiliary components in the solution comprise the following components in percentage by weight:
calcium phosphate, monopotassium phosphate and ammonium nitrate, each 2 g/L;
0.5g/L of zinc glycinate;
500mg/L of humic acid;
10mg/L of each of caproic acid diethylamino ethanol ester and sodium borate;
choline chloride and alpha-sodium naphthaleneacetate, wherein each amount is 0.1 g/L;
0.5g/L of 5-aminolevulinic acid;
2mg/L of compound sodium nitrophenolate and n-triacontanol respectively;
sophorolipid, 5 g/L;
ethanol, 5 mL/L;
salicylic acid, 50 mg/L;
0.5g/L sodium lignosulfonate;
sorbitol, 0.5 g/L.
Example 2
A composite drought-resistant and stress-resistant regulator for wheat is composed of seed-soaking inducer A for treating wheat seeds and drought-resistant and stress-resistant regulator B for spraying it on wheat leaves.
The seed soaking inducer A comprises the following components in percentage by weight: 4 wt% of betaine, 4 wt% of linear inulin polysaccharide, 2 wt% of chitosan, 0.02 wt% of brassinolide, 0.03 wt% of sodium nitroprusside, 0.07 wt% of fulvic acid, 0.02 wt% of titanium citrate and the balance of distilled water, and the volume is fixed to 100 ml.
The drought-resistant stress-resistant regulator B is prepared by the following steps:
diluting 100ml of the inducer A solution into 0.8L of solution by using deionized water, adding a proper amount of foliar nutrient components and auxiliary stress-resistant components C, fully dissolving to prepare a compound solution, uniformly stirring, adding foliar spraying auxiliary components into the compound solution, supplementing inulin polysaccharide to 4g/L, uniformly stirring, and fixing the volume of the deionized water to 1L, thereby preparing a drought-resistant stress-resistant regulator B solution, wherein the foliar nutrient components, the auxiliary stress-resistant components C and the foliar spraying auxiliary components in the solution comprise the following components in content:
calcium phosphate, potassium dihydrogen phosphate, each 3 g/L;
1g/L of zinc glycinate and calcium glycinate respectively;
humic acid, 800 mg/L;
caproic acid diethylaminoethanol ester and sodium borate, each of which is 30 mg/L;
choline chloride and alpha-sodium naphthaleneacetate, wherein each amount is 0.2 g/L;
1g/L of 5-aminolevulinic acid;
n-triacontanol, prohexadione calcium, each 5 mg/L;
rhamnolipid, 5 g/L;
propanol, 10 mL/L;
salicylic acid, 80 mg/L;
0.6g/L sodium lignosulfonate;
sorbitol, 0.8 g/L.
Example 3
A composite drought-resistant and stress-resistant regulator for wheat is composed of seed-soaking inducer A for treating wheat seeds and drought-resistant and stress-resistant regulator B for spraying it on wheat leaves.
The seed soaking inducer A comprises the following components in percentage by weight: 5 wt% of betaine, 5 wt% of linear inulin polysaccharide, 3 wt% of chitosan, 0.03 wt% of brassinolide, 0.05 wt% of sodium nitroprusside, 0.1 wt% of fulvic acid, 0.03 wt% of titanium citrate and the balance of distilled water, and the volume is fixed to 100 ml.
The drought-resistant stress-resistant regulator B is prepared by the following steps:
diluting 100ml of the inducer A solution into 0.8L of solution by using deionized water, adding a proper amount of foliar nutrient components and auxiliary stress-resistant components C, fully dissolving to prepare a compound solution, uniformly stirring, adding foliar spraying auxiliary components into the compound solution, supplementing fructosan to 5g/L, uniformly stirring, and fixing the volume of the deionized water to 1L, thereby preparing a drought-resistant stress-resistant regulator B solution, wherein the foliar nutrient components, the auxiliary stress-resistant components C and the foliar spraying auxiliary components in the solution comprise the following components in content:
potassium dihydrogen phosphate and ammonium nitrate, each 7 g/L;
zinc glycinate and calcium glycinate, each 2 g/L;
humic acid, 1000 mg/L;
50mg/L of each of caproic acid diethylaminoethanol ester and sodium borate;
choline chloride and alpha-sodium naphthaleneacetate, wherein each amount is 0.5 g/L;
2g/L of 5-aminolevulinic acid;
10mg/L of compound sodium nitrophenolate and 10mg/L of prohexadione calcium respectively;
algal glycolipid, 8 g/L;
propanol, 15 mL/L;
salicylic acid, 100 mg/L;
1g/L sodium lignosulfonate;
sorbitol, 1 g/L.
Application examples
The procedure for wheat seed treatment using the seed soaking inducer A described in example 1 was as follows:
s1: seed soaking pretreatment:
diluting 100ml of inducer A solution in example 1 by 10 times to 1L with deionized water, adding 1 wt% of sophorolipid biosurfactant, uniformly stirring, and soaking seeds for 6h at 25 ℃, wherein the weight ratio of the diluted inducer solution to the seeds is 1: 1.5;
s2: surface spraying treatment:
after the seed soaked is subjected to ventilation drying treatment, the undiluted inducer A solution is uniformly sprayed to carry out surface spraying treatment on the seed, and then the seed is subjected to rapid ventilation drying; wherein the weight ratio of the inducer A solution to the seeds is 1: 18.
comparative example 1
The preparation was the same as in example 1, except that inducer A and regulator B did not contain a fructan component.
Comparative example 2
The preparation method was the same as example 1 except that the regulator B contained no fructan component and no foliar nutrient component.
Comparative example 3
The preparation method is the same as that in example 1, except that the regulator B does not contain the auxiliary stress-resistant component C and does not contain the foliage spray auxiliary component.
Effects of the embodiment
The wheat seeds used in the test are No. 17 of Jinan, and the reagents such as sodium nitroprusside, betaine, humic acid and the like are commercially obtained by enterprises such as Sigma company and the like.
The test method comprises the following steps:
seed germination experiment:
selecting wheat seeds which are full in shape and similar in size, disinfecting and cleaning the wheat seeds, putting the wheat seeds into a water bath kettle at 42 ℃ for accelerating germination for 3 hours, ventilating air to dry the surface water of the seeds, dividing the wheat seeds into three experimental groups (simulating stress, treating the seeds by using an inducer in examples 1-3), three control groups (simulating stress, treating the seeds by using the inducer in comparative examples 1-3) and a blank control group (simulating stress and treating without the inducer) and a normal group (non-stress condition and treating without the inducer), then carrying out seed soaking and foliage spraying treatment according to the seed treatment method disclosed by the invention, wherein 200 seeds in each group are repeatedly arranged, and the two groups are placed on a seed germination bed padded with filter paper and gauze to carry out seed germination tests.
Wherein, the conventional solid or liquid culture medium with 15 percent of PEG6000 content is used for cultivating the simulated drought stress environment. The setting conditions of the germination bed are (25 +/-1) DEG C, the illumination time is 12h, and the illumination intensity is 2400 lx.
Seedling leaf treatment experiment:
after seedling emergence (2 leaves), spraying and treating the leaf surfaces of the seedlings once by using respective regulator B (diluted by 50 times of deionized water) every two days, wherein the spraying and treating are carried out until the leaves are completely wet every time, and the liquid of the leaves is controlled not to drip (wherein, the blank control group does not carry out leaf surface treatment, and the normal control group sprays the leaves by using distilled water); wherein the temperature of 7-8 days after seedling emergence is changed to 8 ℃ to simulate the low-temperature stress of seedlings; the illumination intensity is changed to 1200lx in 9-10 days, and the illumination time is changed to 8h to simulate the low light stress; after 12 days, the germination (%) of the seeds, the vigor index (average fresh weight of the seedling plants, g), and the average soluble sugar content (mg/g) and the average proline content (μ g/g) of the seedling leaves were calculated.
The results are shown in tables 1 and 2 below, respectively.
TABLE 1 wheat seed Germination
Experiments show that drought stress causes the reduction of the germination capacity and the vitality index of seeds. After the regulator is used for treatment, the seed germination indexes are improved to different degrees, the inhibition effect of drought stress on seed germination can be remarkably relieved, and the seed germination is remarkably different and changed compared with a control group. The inhibition effect of the product of the invention caused by drought stress can be relieved, and the germination of wheat seeds in the drought environment can be effectively promoted.
TABLE 2 index of seedlings
Soluble sugar and proline are important organic osmotic adjusting substances in plants, and the accumulation of the soluble sugar and the proline in wheat seedlings is induced by drought stress. As can be seen from the seedling index data, in the process of accumulating the osmoregulation substances under drought stress and low-temperature stress, the effect of the regulator treatment group containing the plant fructan and the foliar nutrient components on promoting the wheat leaf osmoregulation substances is obviously higher than that of other control groups, the effect difference on the control groups is obvious, particularly the accumulation effect of proline is higher than that of soluble sugar, and the regulator containing the plant fructan and the foliar nutrient components can obviously reduce the influence of environmental stress on wheat.
It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. In addition, while the invention has been described in detail in the foregoing by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that certain modifications and improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (5)
1. A wheat drought-resistant stress-resistant regulator compound comprises a seed soaking inducer A for wheat seed treatment and a drought-resistant stress-resistant regulator B for wheat foliage spraying; the seed soaking inducer is characterized by comprising the following components in percentage by weight: 3-5 wt% of betaine, 2-5 wt% of fructan, 1-3 wt% of chitosan, 0.01-0.03 wt% of brassinolide, 0.01-0.05 wt% of sodium nitroprusside, 0.05-0.1 wt% of fulvic acid, 0.01-0.03 wt% of titanium citrate and the balance of distilled water; the drought-resistant stress-resistant regulator B for foliage spraying comprises the inducer A solution diluted by 8-10 times of deionized water, a foliage nutrient component, an auxiliary stress-resistant component C, a foliage spraying auxiliary component and supplementary fructan;
wherein the foliar nutrient components are selected from at least two of calcium phosphate, monopotassium phosphate and ammonium nitrate; the fructan is a plant fructan selected from linear fructan or branched fructan;
the auxiliary stress-resistant component C comprises humic acid, glycine chelate, diethylaminoethanol caproate, sodium borate, alpha-sodium naphthaleneacetate, choline chloride, 5-aminolevulinic acid and optional other growth regulators, wherein the other growth regulators are at least one of compound sodium nitrophenolate, n-triacontanol and prohexadione calcium; the foliar spray auxiliary component comprises glycolipid biosurfactant, sodium lignosulfonate, sorbitol, a small amount of organic solvent and a pH regulator, wherein the organic solvent is selected from ethanol or propanol, and the pH regulator is salicylic acid; the glycine chelate is at least one of calcium glycinate and zinc glycinate;
wherein, the drought-resistant stress-resistant regulator B is prepared by the following steps:
diluting an inducer A solution with deionized water, adding a pre-prepared foliar nutrient component and an auxiliary stress-resistant component C, fully dissolving to prepare a compound solution, uniformly stirring, adding a foliar spraying auxiliary component into the compound solution, supplementing the fructan to 3-5g/L, uniformly stirring, and fixing the volume with deionized water to obtain a drought-resistant stress-resistant regulator B solution; wherein, in the regulator B solution, the content of the nutrient components on the leaf surface is 1-10g/L, the content of other growth regulators is 1-20mg/L, and the content of glycolipid biosurfactants is 3-10 g/L.
2. A method for regulating the growth of wheat based on the compound of claim 1, which comprises using a seed soaking inducer A for wheat seed treatment and using a drought-resistant stress-resistant regulator B solution for wheat foliar spray treatment; wherein the wheat seed treatment comprises the steps of seed soaking pretreatment and seed surface spraying treatment.
3. The method of claim 2, wherein the seed soaking inducer A is used for the treatment of wheat seeds by the following steps:
s1: seed soaking pretreatment:
diluting the inducer A solution by 10-15 times with deionized water, adding 1-1.5 wt% glycolipid biosurfactant as a suspension auxiliary agent, uniformly stirring, and soaking seeds for 4-8h at 25-30 ℃, wherein the weight ratio of the diluted inducer solution to the seeds is 1: 1-1.5;
s2: surface spraying treatment:
after the seed is subjected to surface ventilation drying treatment, uniformly spraying undiluted inducer A solution, performing surface spraying treatment on the seed, and quickly performing ventilation drying after the surface spraying treatment; wherein the weight ratio of the inducer A solution to the seeds is 1: 10-20.
4. The method of claim 2, wherein the wheat foliar spray treatment step is: diluting the drought-resistant stress-resistant regulator B solution by using deionized water with the weight of 50-200 times to form a spraying solution, and uniformly spraying the spraying solution on the surfaces of leaves of wheat seedling plants.
5. Use of the wheat drought tolerance stress-resistance regulator complex as defined in claim 1 for regulating the growth of wheat.
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| CN113951262A (en) * | 2021-10-18 | 2022-01-21 | 山西省农业科学院棉花研究所 | Preparation for improving stress resistance potential and post-disaster recovery of wheat and application thereof |
| CN114521555B (en) * | 2022-01-26 | 2024-02-27 | 中国农业科学院作物科学研究所 | Preparation and application of sorghum stress-resistance lodging-resistance regulator |
| CN114521558A (en) * | 2022-02-24 | 2022-05-24 | 百色学院 | Sweet corn seed soaking agent and preparation method and application thereof |
| CN114885771B (en) * | 2022-04-28 | 2023-05-05 | 西藏自治区农牧科学院农业研究所 | Cultivation method for improving stress resistance of wheat |
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