CN112358352B - Composite preparation for improving osmotic stress resistance of rice seedlings and preparation method and application thereof - Google Patents
Composite preparation for improving osmotic stress resistance of rice seedlings and preparation method and application thereof Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 117
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 108
- 235000009566 rice Nutrition 0.000 title claims abstract description 108
- 230000008723 osmotic stress Effects 0.000 title claims abstract description 78
- 239000002131 composite material Substances 0.000 title description 17
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 108
- XKGUZGHMWUIYDR-UHFFFAOYSA-N benzyl n-(3-fluoro-4-morpholin-4-ylphenyl)carbamate Chemical compound C=1C=C(N2CCOCC2)C(F)=CC=1NC(=O)OCC1=CC=CC=C1 XKGUZGHMWUIYDR-UHFFFAOYSA-N 0.000 claims abstract description 66
- 150000001875 compounds Chemical class 0.000 claims abstract description 61
- BWILYWWHXDGKQA-UHFFFAOYSA-M potassium propanoate Chemical compound [K+].CCC([O-])=O BWILYWWHXDGKQA-UHFFFAOYSA-M 0.000 claims abstract description 27
- 235000010332 potassium propionate Nutrition 0.000 claims abstract description 27
- 239000004331 potassium propionate Substances 0.000 claims abstract description 27
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 25
- 239000011591 potassium Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 72
- 239000002904 solvent Substances 0.000 claims description 16
- 238000009472 formulation Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
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- 239000003337 fertilizer Substances 0.000 claims description 3
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- 230000000996 additive effect Effects 0.000 claims description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 abstract description 68
- 235000011056 potassium acetate Nutrition 0.000 abstract description 34
- WFIZEGIEIOHZCP-UHFFFAOYSA-M potassium formate Chemical group [K+].[O-]C=O WFIZEGIEIOHZCP-UHFFFAOYSA-M 0.000 abstract description 24
- 230000012010 growth Effects 0.000 abstract description 10
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 34
- 241000196324 Embryophyta Species 0.000 description 18
- 229930002875 chlorophyll Natural products 0.000 description 15
- 235000019804 chlorophyll Nutrition 0.000 description 15
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 15
- 239000002689 soil Substances 0.000 description 15
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 13
- 229910052799 carbon Inorganic materials 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- -1 organic acid radical Chemical class 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 7
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 239000001099 ammonium carbonate Substances 0.000 description 6
- 235000012501 ammonium carbonate Nutrition 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 238000010998 test method Methods 0.000 description 5
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 4
- 235000011130 ammonium sulphate Nutrition 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 229940093429 polyethylene glycol 6000 Drugs 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 3
- 238000013329 compounding Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000008641 drought stress Effects 0.000 description 2
- 238000009328 dry farming Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 150000001722 carbon compounds Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 229940106705 chlorophyll Drugs 0.000 description 1
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- 238000006731 degradation reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000020774 essential nutrients Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 238000002791 soaking Methods 0.000 description 1
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- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a compound preparation for improving osmotic stress resistance of rice seedlings and a preparation method and application thereof. The compound preparation consists of ammonium gluconate and micromolecular organic potassium; the organic potassium salt is potassium formate, potassium acetate or potassium propionate. The compound preparation can effectively improve the osmotic stress resistance of the rice seedlings, improve the drought resistance of the rice seedlings and promote the growth of the rice seedlings.
Description
Technical Field
The invention relates to a compound preparation for improving osmotic stress resistance of rice seedlings and a preparation method and application thereof, belonging to the technical field of drought stress resistance of crops.
Background
China is one of the countries with serious water shortage in the world, and the water resource people all occupy 109 th world and are listed as one of 13 countries with serious water shortage in the world; the drought area occupies more than 1/2 of the territorial area of China, the annual rainfall is 50-250 mm, the soil moisture is very deficient, and the crop growth has huge loss every year due to drought. Drought is the most important abiotic stress factor affecting agricultural and forestry production, and osmotic stress caused by drought is one of the important causes of crop injury.
Agricultural water in China accounts for about 70% of the total water. Rice is one of the main grain crops in China and is planted in large area in China, but water resources are seriously wasted by traditional flooding irrigation, so that the rice dry farming is realized, and the rice dry farming method has important significance for saving water resources and increasing grain yield, and is beneficial to saving energy and improving increasingly deteriorated ecological environment.
Nitrogen is one of the most important nutrient elements essential for plant growth and life activity, and is the component of important organic compound in plant body, such as protein, nucleic acid, chlorophyll, enzyme, vitamin, hormone, etc. Among all essential nutrients, nitrogen is the primary factor limiting plant growth and yield development worldwide and has a clear effect on improving crop product quality. Nitrogen fertilizer is also one of the most effective factors in increasing crop yield.
Soil organic carbon and soil structure are important indexes for measuring soil fertility and are also main factors for influencing soil stability and productivity. The organic carbon in the soil has three main functions, namely, important sources of crop nutrients, improvement of soil storage and buffering performance and improvement of soil physical properties.
Therefore, the development of an anti-drought preparation which can provide a nitrogen source and an organic carbon source for rice has important research significance.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a composite preparation for improving osmotic stress resistance of rice seedlings and a preparation method and application thereof. The composite preparation is prepared by compounding ammonium gluconate with micromolecule organic potassium with different carbon numbers, and the obtained composite preparation can effectively improve the osmotic stress resistance of rice seedlings, improve the drought resistance of the rice seedlings and promote the growth of the rice seedlings.
The technical scheme of the invention is as follows:
a compound preparation for improving osmotic stress resistance of rice seedlings is composed of ammonium gluconate and micromolecular organic potassium; the molar ratio of the ammonium gluconate to the small molecular organic potassium is 2-13; the organic potassium salt is potassium formate, potassium acetate or potassium propionate.
According to the invention, the molar ratio of the ammonium gluconate to the small-molecule organic potassium is 3-10.
According to the invention, the molar ratio of the ammonium gluconate to the small-molecule potassium organate is preferably 4-7:1.
According to the present invention, the ammonium gluconate may be obtained commercially or prepared according to the prior art. The potassium formate, acetate and propionate can be obtained commercially or prepared according to the prior art.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and small molecular organic potassium.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings.
According to the invention, the compound preparation can be used alone or used as an additive to be compatible with fertilizer or culture solution.
Preferably, the complex formulation is used when the rice seeds grow to have two leaves and one heart.
According to the invention, the usage amount of the compound preparation is as follows: the water is used as solvent, and each liter of water contains 0.5-5.0 g of ammonium gluconate and 0.03-0.5 g of micromolecular organic acid potassium. Preferably, when the micromolecular organic potassium formate is potassium formate, the content of the potassium formate in each liter of water is 0.03-0.34 g; when the micromolecular organic potassium is potassium acetate, the content of the potassium acetate in each liter of water is 0.04-0.4 g; when the micromolecular organic potassium is potassium propionate, the content of the potassium propionate in each liter of water is 0.05-0.45 g.
Preferably, the usage amount of the compound preparation is as follows: water is used as a solvent, and each liter of water contains 1.0 to 4.0g of ammonium gluconate and 0.06 to 0.35g of micromolecular organic potassium. Further preferably, when the micromolecular organic potassium formate is potassium formate, the content of potassium formate in each liter of water is 0.06-0.26 g; when the micromolecular organic potassium is potassium acetate, the content of the potassium acetate in each liter of water is 0.08-0.4 g; when the micromolecular organic potassium is potassium propionate, the content of the potassium propionate in each liter of water is 0.1-0.35 g.
Further preferably, the usage amount of the compound preparation is as follows: the water is used as solvent, and each liter of water contains 1.5-3.0 g of ammonium gluconate and 0.09-0.23 g of micromolecular organic acid potassium. Further preferably, when the micromolecular organic potassium formate is potassium formate, the content of potassium formate in each liter of water is 0.09-0.17 g; when the micromolecular organic potassium is potassium acetate, the content of the potassium acetate in each liter of water is 0.12-0.2 g; when the micromolecular organic potassium is potassium propionate, the content of the potassium propionate in each liter of water is 0.15-0.35 g.
According to the invention, the method for simulating osmotic stress is as follows:
PEG (polyethylene glycol 6000) is dissolved in water to prepare osmotic stress solution with the mass concentration of 10%.
Using 3% of H for rice seeds 2 O 2 Sterilizing the water solution for 10min, and washing with distilled water for 5 times; then immersing the seeds inSoaking in distilled water for 12h, draining, and culturing in a germination machine; when the seedlings grow to have two leaves and one heart, selecting the rice seedlings with consistent growth vigor, transplanting the rice seedlings into an incubator, and adding a conventional nutrient solution of an International Rice institute (IRRI) to perform water culture treatment (12-hour illumination and 12-hour dark alternation, and the temperature is 20-25 ℃) for 5 days; then adding the composite preparation of the invention at one time, and continuing the water culture treatment for 5 days according to the conditions; then, PEG was added to the culture solution to a concentration of 10wt%, and the pH of the culture solution was adjusted to 5.5. + -. 0.1 with 1mol/L HCl and 1mol/L NaOH aqueous solutions, and the culture solution was renewed 1 time every 3 days and subjected to osmotic stress for 10 days.
The invention has the following technical characteristics and beneficial effects:
1. the compound preparation is applied to osmotic stress resistance of rice seedlings, and can provide a nitrogen source, a carbon source and a potassium source at the same time. Besides providing nitrogen-containing nutrient elements, the ammonium gluconate has stronger permeability adjustment capability on rice due to special ammonium nitrogen, can improve or maintain the water content of leaves, and has adjustment effect on root growth and xylem sap, so that the drought resistance of the rice can be improved. The ammonium gluconate is used as a carbon source, and compared with glucose, the microorganisms cannot rapidly generate CO after utilizing the glucose acid radicals 2 The organic carbon is released and changed into short-chain carbon compounds for storage, thereby being more beneficial to improving the content of organic carbon in soil and further relieving the soil degradation caused by the reduction of the organic carbon in the soil.
2. The organic acid radical contained in the micromolecular organic potassium salt not only can be beneficial to the plant to resist osmotic stress and promote the plant growth, but also can improve the soil and relieve the adverse effect caused by excessive fertilization; and K in small molecular organic potassium + The nutrient elements necessary for the growth of plants can achieve the purpose of reducing the amount of fertilizer for application. Organic acid radicals with different carbon numbers have great influence on the growth and development of plant roots, the chlorophyll content, the photosynthetic efficiency and the like; the organic acid radicals with different carbon numbers have different stimulation effects on the growth and development of plant roots and leaves; the composite preparation of the invention combines the glucose acid radical and the specific organic acid radical, NH 4 + And K + The compound use can effectively improve the osmotic stress resistance of the rice seedlings,under the drought condition, the rice antioxidant enzyme activity can be enhanced, the stability of cell membranes is maintained, the fresh weight, the plant height, the chlorophyll content of seedlings, the leaf area and the root activity of plants are obviously improved, the MDA content is reduced, the growth of rice seedlings is obviously promoted, and the yield is increased.
3. The compound preparation is applied to osmotic stress resistance of rice seedlings, can effectively improve the drought stress resistance of the rice seedlings and promote the growth of the rice seedlings. The composite preparation is simple and easy to apply, ecological and environment-friendly, and does not introduce inorganic acid radical ions, so that the ion osmotic stress caused by soil hardening or salinization caused by fertilization is avoided; can effectively improve soil fertility, improve soil microbial activity, provide nutrients for the growth of rice seedlings and further improve crop yield.
4. The excellent effect of the invention can be realized only by compounding the specific raw materials, namely the ammonium gluconate and the small molecular organic acid, and the excellent effect of the invention can not be realized by similar replacement of any one raw material. The ammonium gluconate and the small molecular organic acid are required to be compounded in a specific ratio, and the excellent effect of the ammonium gluconate and the small molecular organic acid cannot be realized even if the compounding ratio is not proper. The addition concentration of the composite preparation of the invention is also proper when the composite preparation is applied, and the excellent effect of the invention can not be realized even if the addition amount is too low or too high.
Detailed Description
The technical solutions of the present invention are further described below with reference to the following examples, but the scope of the present invention is not limited thereto.
Raw materials used in the examples are all conventional products unless otherwise specified; the equipment used is conventional equipment unless otherwise specified.
The used ammonium gluconate is prepared according to the method of example 2 in Chinese patent document CN 109022507A; potassium formate, potassium acetate, potassium propionate are commercially available or can be prepared according to the prior art.
Example 1
A compound preparation for improving osmotic stress resistance of rice seedlings is composed of ammonium gluconate and potassium formate; the molar ratio of ammonium gluconate to potassium formate is 6.58.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and potassium formate.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: water is used as a solvent, and each liter of water contains 1g of ammonium gluconate and 0.06g of potassium formate.
The method for simulating osmotic stress is as follows: mixing rice seed (Nanjing 9108) with 3% of H 2 O 2 Sterilizing the solution for 10min, washing with distilled water for 5 times, soaking the seeds in distilled water for 12 hr, draining, and culturing in a germination machine; when the rice seedlings grow to have two leaves and one heart, selecting the rice seedlings with consistent growth vigor, transplanting the rice seedlings into an incubator, and adding a conventional nutrient solution of the International Rice institute (IRRI) to perform water culture treatment (12 h illumination and 12h dark alternation, water culture temperature is 20-25 ℃) for 5 days; then adding the compound preparation of the embodiment to continue the water culture treatment for 5 days according to the conditions (the adding amount of each liter of water contains 1g of ammonium gluconate and 0.06g of potassium formate); then PEG was added to make the concentration in the culture solution to 10wt%, the pH of the culture solution was adjusted to 5.5. + -. 0.1 with 1mol/L HCl and 1mol/L NaOH aqueous solutions, and the culture solution was renewed 1 time every 3 days and subjected to osmotic stress for 10 days.
The culture was carried out as described above, and a control group without ammonium gluconate and potassium formate was set.
The physiological indexes of the rice seedlings obtained by the tests are determined, and the results show that: compared with a control group, the fresh weight of the plant is improved by 9.7 percent compared with the control group, the MDA is reduced by 10.3 percent compared with the control group, the chlorophyll content of the seedling is improved by 18.1 percent compared with the control group, the leaf area is improved by 26.3 percent compared with the control group, and the root system activity is improved by 14.1 percent compared with the control group; the compound preparation of the embodiment can effectively improve the osmotic stress resistance of the rice seedlings.
Example 2
A compound preparation for improving osmotic stress resistance of rice seedlings, which consists of ammonium gluconate and potassium acetate; the molar ratio of ammonium gluconate to potassium acetate was 5.83.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and potassium acetate.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: the water is used as a solvent, and each liter of water contains 1.5g of ammonium gluconate and 0.12g of potassium acetate.
The simulated osmotic stress test was consistent with example 1, except that: the addition amount of the composite preparation in the embodiment is as follows: the water per liter contains 1.5g of ammonium gluconate and 0.12g of potassium acetate. A control group without ammonium gluconate and potassium acetate was also set.
The physiological indexes of the rice seedlings obtained by the tests are determined, and the results show that: compared with a control group, the fresh weight of the plant is improved by 27.2 percent compared with the control group, the MDA is reduced by 29.1 percent compared with the control group, the chlorophyll content of the seedling is improved by 41.5 percent compared with the control group, the leaf area is improved by 50.6 percent compared with the control group, and the root system activity is improved by 40.2 percent compared with the control group; the compound preparation of the embodiment can effectively improve the osmotic stress resistance of the rice seedlings.
Example 3
A compound preparation for improving osmotic stress resistance of rice seedlings, which consists of ammonium gluconate and potassium propionate; the molar ratio of ammonium gluconate to potassium propionate is 4.48.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and potassium propionate.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the usage amount of the compound preparation is as follows: water is used as a solvent, and each liter of water contains 2g of ammonium gluconate and 0.23g of potassium propionate.
The simulated osmotic stress test was consistent with example 1, except that: the addition amount of the composite preparation in the embodiment is as follows: each liter of water contains 2g of ammonium gluconate and 0.23g of potassium propionate. A control group was also set without the addition of ammonium gluconate and potassium propionate.
The results of the determination of the physiological indexes of the rice seedlings obtained by the test method show that: compared with a control group, the fresh weight of the plant is improved by 34.5 percent compared with the control group, the MDA is reduced by 37.2 percent compared with the control group, the chlorophyll content of the seedling is improved by 53.8 percent compared with the control group, the leaf area is improved by 60.4 percent compared with the control group, and the root system activity is improved by 51.7 percent compared with the control group; the compound preparation of the embodiment can effectively improve the osmotic stress resistance of the rice seedlings.
Example 4
A composite preparation for improving osmotic stress resistance of rice seedlings is composed of ammonium gluconate and potassium propionate; the molar ratio of ammonium gluconate to potassium propionate was 4.52.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and potassium propionate.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: water is used as a solvent, and each liter of water contains 3g of ammonium gluconate and 0.35g of potassium propionate.
The simulated osmotic stress test was consistent with example 1, except that: the addition amount of the composite preparation in the embodiment is as follows: each liter of water contains 3g of ammonium gluconate and 0.35g of potassium propionate. A control group was also set without the addition of ammonium gluconate and potassium propionate.
The results of the determination of the physiological indexes of the rice seedlings obtained by the test method show that: compared with a control group, the fresh weight of the plant is increased by 16.1 percent compared with the control group, the MDA is reduced by 18.3 percent compared with the control group, the chlorophyll content of the seedling is increased by 26.7 percent compared with the control group, the leaf area is increased by 30.2 percent compared with the control group, and the root system activity is increased by 23.7 percent compared with the control group; the compound preparation of the embodiment can effectively improve the osmotic stress resistance of the rice seedlings.
Example 5
A compound preparation for improving osmotic stress resistance of rice seedlings, which consists of ammonium gluconate and potassium acetate; the molar ratio of ammonium gluconate to potassium acetate was 4.63.
The preparation method of the compound preparation for improving the osmotic stress resistance of the rice seedlings comprises the following steps: mixing ammonium gluconate and potassium acetate.
The application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: water is used as a solvent, 4g of ammonium gluconate is contained in each liter of water, and the content of potassium acetate is 0.4g.
The simulated osmotic stress test was consistent with example 1, except that: the addition amount of the composite preparation in the embodiment is as follows: each liter of water contains 4g of ammonium gluconate and 0.4g of potassium acetate. A control group without ammonium gluconate and potassium acetate was also set.
The results of the determination of the physiological indexes of the rice seedlings obtained by the test method show that: compared with a control group, the fresh weight of the plant is improved by 6.5 percent compared with the control group, the MDA is reduced by 8.6 percent compared with the control group, the chlorophyll content of the seedling is improved by 12.6 percent compared with the control group, the leaf area is improved by 17.4 percent compared with the control group, and the root system activity is improved by 10.5 percent compared with the control group; the compound preparation of the embodiment can effectively improve the osmotic stress resistance of the rice seedlings.
Comparative example 1
A complex formulation consisting of ammonium sulfate and potassium acetate; the application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: the solvent was water containing 0.46g of ammonium sulfate (molar concentration of ammonium ion was the same as that in example 2) per liter of water, and the content of potassium acetate was 0.12g.
The simulated osmotic stress test was consistent with example 2, except that: the addition amount of the composite preparation of the comparative example is as follows: the water contained ammonium sulfate (0.46 g/L) (molar concentration of ammonium ion was the same as that of example 2) and potassium acetate (0.12 g/L). A control group without ammonium sulfate and potassium acetate was also set.
The physiological indexes of the rice seedlings obtained by the culture method are measured, and the results show that: compared with a control group, the fresh weight of the plant is improved by 3.7 percent compared with the control group, MDA has no significant difference compared with the control group, the chlorophyll content of the seedling is improved by 5.8 percent compared with the control group, the leaf area is improved by 8.3 percent compared with the control group, and the root activity is improved by 4.8 percent compared with the control group. The above effect data are far inferior to that of example 2 of the present invention, which shows that the effect of the present invention is not achieved by any ammonium ion-containing formulation, and that only the specific ammonium gluconate formulation of the present invention is effective in improving the osmotic stress resistance of rice seedlings.
Comparative example 2
A complex formulation consisting of ammonium carbonate and potassium acetate; the application of the compound preparation for improving the osmotic stress resistance of the rice seedlings is applied to improving the osmotic stress resistance of the rice seedlings; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: the solvent was water containing 0.34g of ammonium carbonate per liter of water (wherein the molar concentration of ammonium ions was the same as that in example 2) and the potassium acetate content was 0.12g.
The simulated osmotic stress test was consistent with example 2, except that: the addition amount of the composite preparation of the comparative example is as follows: the water contained 0.34g of ammonium carbonate per liter (wherein the molar concentration of ammonium ions was the same as that in example 2) and the potassium acetate content was 0.12g per liter. A control group without ammonium carbonate and potassium acetate was also set.
The physiological indexes of the rice seedlings obtained by the culture method are measured, and the results show that: compared with a control group, the fresh weight of the plant is reduced by 4.3 percent compared with the control group, the MDA is increased by 5.9 percent compared with the control group, the chlorophyll content of the seedling has no significant difference with the control group, the leaf area is reduced by 10.5 percent compared with the control group, and the root activity is reduced by 7.2 percent compared with the control group; the effect data show that ammonium carbonate can be used as a carbon source and a nitrogen source, but the addition of ammonium carbonate has no effect or even generates adverse effect on osmotic stress resistance of rice seedlings, and further shows that the effect of the invention can not be realized by any preparation containing ammonium ions and the carbon source, and only the specific ammonium gluconate preparation can effectively improve the osmotic stress resistance of the rice seedlings.
Comparative example 3
A formulation consisting solely of ammonium gluconate; the application of the preparation is applied to improving the osmotic stress resistance of rice seedlings; the preparation is used when rice seeds grow to have two leaves and one heart; the dosage of the preparation is as follows: the water is used as a solvent, and each liter of water contains 1.5g of ammonium gluconate.
The simulated osmotic stress test was consistent with example 2, except that: the addition amount of the comparative example preparation was: the water per liter contains 1.5g of ammonium gluconate. A control group without ammonium gluconate was also set.
The physiological indexes of the rice seedlings obtained by the tests are determined, and the results show that: compared with a control group, the fresh weight of the plant is increased by 19.1 percent compared with the control group, the MDA is reduced by 20.4 percent compared with the control group, the chlorophyll content of the seedling is increased by 29.2 percent compared with the control group, the leaf area is increased by 35.4 percent compared with the control group, and the root system activity is increased by 28.3 percent compared with the control group; the single ammonium gluconate preparation in the comparative example can improve the osmotic stress resistance of the rice seedlings, but has an obvious effect which is not as good as that of a compound preparation matched with the micromolecular organic potassium.
Comparative example 4
A formulation consisting solely of ammonium gluconate; the application of the preparation is applied to improving the osmotic stress resistance of rice seedlings; the preparation is used when rice seeds grow to have two leaves and one heart; the dosage of the preparation is as follows: water is used as a solvent, and each liter of water contains 2g of ammonium gluconate.
The simulated osmotic stress test was consistent with example 3, except that: the addition amount of the comparative example preparation was: each liter of water contains 2g of ammonium gluconate. A control group without ammonium gluconate was also set.
The results of the determination of the physiological indexes of the rice seedlings obtained by the test method show that: compared with a control group, the fresh weight of the plant is increased by 24.5 percent compared with the control group, the MDA is reduced by 26.3 percent compared with the control group, the chlorophyll content of the seedling is increased by 37.7 percent compared with the control group, the leaf area is increased by 42.4 percent compared with the control group, and the root system activity is increased by 36.1 percent compared with the control group; the single ammonium gluconate preparation in the embodiment can improve the osmotic stress resistance of the rice seedlings, but has an obvious effect inferior to that of a compound preparation matched with the small-molecular organic potassium.
Comparative example 5
A formulation consisting of potassium propionate alone; the preparation is applied to improving the osmotic stress resistance of rice seedlings; the preparation is used when rice seeds grow to have two leaves and one heart; the dosage of the preparation is as follows: the solvent is water, and each liter of water contains 0.23g of potassium propionate.
The simulated osmotic stress test was consistent with example 3, except that: the addition amount of the comparative example preparation was: the water content per liter is 0.23g of potassium propionate. A control group without potassium propionate was also set.
The results of the determination of the physiological indexes of the rice seedlings obtained by the test method show that: compared with a control group, the fresh weight of the plant is increased by 8.6 percent compared with the control group, the MDA is reduced by 8.8 percent compared with the control group, the chlorophyll content of the seedling is increased by 12.1 percent compared with the control group, the leaf area is increased by 13.8 percent compared with the control group, and the root system activity is increased by 7.6 percent compared with the control group; the single potassium propionate preparation in this example can improve the osmotic stress resistance of rice seedlings, but the effect is obviously inferior to that of the compound preparation matched with ammonium gluconate.
Comparative example 6
A formulation consisting solely of potassium acetate; the preparation is applied to improving the osmotic stress resistance of rice seedlings; the preparation is used when rice seeds grow to have two leaves and one heart; the dosage of the preparation is as follows: the solvent is water, and each liter of water contains 0.12g of potassium acetate.
The simulated osmotic stress test was consistent with example 2, except that: the addition amount of the comparative example preparation was: the water contained potassium acetate 0.12g per liter. A control group without potassium acetate was also set.
The physiological indexes of the rice seedlings obtained by the tests are determined, and the results show that: compared with a control group, the fresh weight of the plant is improved by 7.2 percent compared with the control group, the MDA is reduced by 8.1 percent compared with the control group, the chlorophyll content of the seedling is improved by 10.6 percent compared with the control group, the leaf area is improved by 11.9 percent compared with the control group, and the root system activity is improved by 6.3 percent compared with the control group; the single potassium acetate preparation in the embodiment can improve the osmotic stress resistance of the rice seedlings, but the effect is obviously inferior to that of a compound preparation matched with the ammonium gluconate.
Comparative example 7
A formulation consisting of potassium formate alone; the application of the preparation is applied to improving the osmotic stress resistance of rice seedlings; the preparation is used when rice seeds grow to have two leaves and one heart; the dosage of the preparation is as follows: the solvent is water, and each liter of water contains 0.06g of potassium formate.
The simulated osmotic stress test was consistent with example 1, except that: the addition amount of the comparative example preparation was: the water per liter contains 0.06g of potassium formate. A control group without potassium formate was also set.
The physiological indexes of the rice seedlings obtained by the tests are determined, and the results show that: compared with a control group, the fresh weight of the plant is improved by 6.8 percent compared with the control group, the MDA is reduced by 7.5 percent compared with the control group, the chlorophyll content of the seedling is improved by 9.7 percent compared with the control group, the leaf area is improved by 11.3 percent compared with the control group, and the root system activity is improved by 6.2 percent compared with the control group; the single potassium formate preparation in this example can improve the osmotic stress resistance of rice seedlings, but the effect is obviously inferior to that of the compound preparation matched with ammonium gluconate.
Claims (3)
1. The application of a compound preparation in improving the osmotic stress resistance of rice seedlings is characterized in that the compound preparation consists of ammonium gluconate and micromolecular organic potassium; the molar ratio of the ammonium gluconate to the small-molecular organic potassium is 4.48; the micromolecular organic potassium is potassium propionate; the compound preparation is used when rice seeds grow to have two leaves and one heart; the use amount of the compound preparation is as follows: water is used as solvent, and each liter of water contains 1.5-3.0 g of ammonium gluconate and 0.15-0.23 g of micromolecular organic acid potassium.
2. The use of the complex formulation of claim 1 for improving osmotic stress resistance of young rice plants, wherein the preparation method of the complex formulation comprises the steps of: mixing ammonium gluconate and small molecular organic potassium.
3. The use of the compound preparation according to claim 1 for improving the osmotic stress resistance of rice seedlings, wherein the compound preparation can be used alone or as an additive for compatibility with a fertilizer or a culture solution.
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