CN108840746A - A kind of efficient foliage bacterial fertilizer of tea tree and the preparation method and application thereof - Google Patents
A kind of efficient foliage bacterial fertilizer of tea tree and the preparation method and application thereof Download PDFInfo
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- CN108840746A CN108840746A CN201810861230.5A CN201810861230A CN108840746A CN 108840746 A CN108840746 A CN 108840746A CN 201810861230 A CN201810861230 A CN 201810861230A CN 108840746 A CN108840746 A CN 108840746A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses efficient foliage bacterial fertilizer of a kind of tea tree and the preparation method and application thereof, 10-20 parts of microbial inoculum containing germ oligotrophy unit cell, 8-12 parts of providencia stuartii microbial inoculum, 8-12 parts of monospore category bacteria agent, 3-7 parts of chitosan, 8-12 parts of amino acid, 3-7 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-40 parts of water.Enhance tea tree disease resistance, the growth for promoting low age and growing into forest tea tree plant etc. applied to the yield, improvement tea tree quality, induction for increasing tea tree.Have many advantages, such as significant effect, green non-pollution, utilization rate of fertilizer height, prevention and control disease, improves yield and quality, is low in cost and easy to spread.
Description
Technical field
The present invention relates to tree plant cultivation technologies and fertilising field, and in particular to a kind of efficient foliage bacterial fertilizer of tea tree and its preparation
Method and application.
Background technique
The poor efficiency of chemical fertilizer and the high environmental risk of its bring are always to perplex the significant problem in the whole world in agricultural production,
Chemical fertilizer use has also resulted in tea place ecological environment severe exacerbation in Tea Production, and tea leaf quality is difficult to ensure.How to make
The special fertilizer of standby tealeaves green ecological, optimizes growth of tea plant environment, improves the yield and quality of tea tree, raw to entire tea place is promoted
The improvement of state system is that tea industry sustainable development is of great significance.And the tea leaf quality of simple application of organic fertilizers production is good,
But yield is not high.Although the tealeaves yield of application inorganic fertilizer production increases substantially merely, quality is declined.And due to tea
Tree is more batches of harvestings in 1 year, relatively continuous and steady to the absorption of nutrient, without obviously peak period.Therefore tea tree
The fertilizer of application should fertilizer efficiency it is long, in order to avoid frequently fertilising cause root system to injure, nutrient supplies stabilization again, to guarantee that blade is equal
It is even, improve tea quality.
Foliage bacterial fertilizer by can be absorbed by plant rapidly by plant leaf blade after being sprayed onto plant leaf surface, avoid from
When soil application nutrient easily by soil consolidation, root absorption is slow the problems such as, have that absorb that fast, nutrient loss is few etc. excellent
Point.Currently, also having certain effect, but all different journeys in the yield for improving crop there are many kinds of existing foliage bacterial fertilizers
The presence of degree improves the defects of crop yield effect is not significant enough.And there are no the ideals designed exclusively for tea tree in the prior art
Foliage bacterial fertilizer.
Summary of the invention
The object of the invention is in view of the deficiencies of the prior art, it is desirable to provide a kind of green non-pollution, meets tea tree at significant effect
The efficient foliage bacterial fertilizer of the tea tree of growing nutrient demand.
Technical scheme is as follows：
A kind of efficient foliage bacterial fertilizer of tea tree, it is characterised in that：It is made from the following raw materials in parts by weight：Thermophilic malt oligotrophy list
10-20 parts of born of the same parents' bacteria agent, 8-12 parts of providencia stuartii microbial inoculum, 8-12 parts of monospore category bacteria agent, 3-7 parts of chitosan, ammonia
8-12 parts of base acid, 3-7 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-40 parts of water.
Preferably, it is made from the following raw materials in parts by weight：15 parts of germ oligotrophy unit cell microbial inoculum, Si Shi Providian
It is 10 parts of this bacteria agent, 10 parts of monospore category bacteria agent, 5 parts of chitosan, 0 part of amino acid 1,5 parts of seaweeds, fertile 15 parts, 30 parts of water micro-.
Preferably, the strain of the germ oligotrophy unit cell microbial inoculum is germ oligotrophy unit cell XQ08 (CCTCC NO:
M 2015726), the strain of providencia stuartii microbial inoculum be providencia stuartii JD (CCTCC NO:M
2017738), the strain of monospore category bacteria agent is monospore category bacterium J3 (CCTCCNO:M2017739).
Wherein, the microbial inoculum preparation includes the following steps：(1) inclined-plane strain X Q08, JD and J3 is saved into test tube seed, point
It is not seeded in minimal medium, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2) by the bacterium solution of acquisition with 10%
The inoculum concentration of percent by volume is seeded in the seed fermentation tank containing minimal medium, and culture to logarithmic growth phase obtains
Seed liquor；(3) seed liquor of acquisition is inoculated in fermentation medium according to the inoculum concentration of 10% volume ratio, 30-35 DEG C,
Fermented and cultured 2-3 days under 150-200rpm, obtain fermentation liquid, the living bacteria count of fermentation liquid >=10,000,000,000/mL, by fermentation liquid
Directly 5-20 times of dilution obtains germ oligotrophy unit cell liquid bacterial agent, providencia stuartii liquid bacterial agent and list respectively
Spore category bacteria liquid microbial inoculum.
Further, the chitosan is water solubility, and deacetylation is greater than 95%；The amino acid is proline, sweet ammonia
Acid, glutamic acid, phenylalanine, leucine, isoleucine, lysine, threonine, methionine, tryptophan, histidine, tyrosine,
Valine, alanine, serine, arginine, asparatate, any five kinds or more of combination in cysteine；Seaweed
Element is pure natural marine algae extract；Micro- fertilizer for mass ratio is:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:
Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer+selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer of 0.5-1；The water is sterile water.
The preparation method of the efficient foliage bacterial fertilizer of tea tree of the invention, comprises the technical steps that：
Step 1. takes germ oligotrophy unit cell liquid bacterial agent, Si Shi Providian described in claim 4 by regulation proportion
This bacteria liquid microbial inoculum and monospore category bacteria liquid microbial inoculum, it is spare；
Step 2. is matched by regulation takes chitosan, amino acid, seaweeds and micro- fertilizer that the nothing for being dissolved in required proportion is sufficiently stirred
In bacterium water；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., in 25-30 DEG C of temperature, pH6.0~6.8
Under conditions of, it is sufficiently mixed in sterile blender uniformly, it is packed up to tea tree blade face bacterial fertilizer products.
Bio-bacterial manure of the invention can be applied in following (1)-(4) at least one：
(1) increase the yield of tea tree；(2) improve tea tree quality, improve tea tree storage performance；(3) induction enhancing tea tree is anti-
Characteristic of disease, the diseases such as effective prevention and control tea tree anthracnose, moire blight, gall of tea；(4) promote the life of low age and the tea tree plant that grows into forest
It is long.
Compared with prior art, the present invention foliage bacterial fertilizer of the present invention uses germ oligotrophy unit cell liquid bacterial agent, Si Shi
The composition such as Providence liquid bacterial agent, monospore category bacteria liquid microbial inoculum, plant growth-promoting substance, a great number of elements and microelement,
Tea tree economic benefit, nutrient and flavour quality can be effectively improved, promotes plant well to grow, and can effectively induce tea tree plant, fruit
The generation of the Major Diseases such as the enhancing of real disease resistance and prevention tea tree anthracnose, moire blight, gall of tea, have significant effect,
Green non-pollution, utilization rate of fertilizer height, prevention and control disease, improve yield and quality, it is low in cost and easy to spread the advantages that.
Specific embodiment
Below with reference to embodiment, the invention will be further described, but not as any limitation of the invention.
Embodiment 1：
1. bio-bacterial manure composition and preparation：
Bio-bacterial manure A formula composition and preparation, (1) composition：15 parts of germ oligotrophy unit cell microbial inoculum, Si Shi Providian
It is 10 parts of this bacteria agent, 10 parts of monospore category bacteria agent, 5 parts of chitosan, 0 part of amino acid 1,5 parts of seaweeds, fertile 15 parts, 30 parts of water micro-.
(2) it prepares：1. taking germ oligotrophy unit cell liquid bacterial agent, providencia stuartii liquid bacterial agent and monospore by regulation proportion
Belong to bacteria liquid microbial inoculum, it is spare；2. taking chitosan, amino acid, seaweeds and micro- fertilizer to be sufficiently stirred by regulation proportion is dissolved in required match
In the sterile water of ratio；3. 1. microbial inoculum that step obtains is added in 2. solution that step obtains, in 25-30 DEG C of temperature, pH 6.0-
Under conditions of 6.8, it is sufficiently mixed in sterile blender uniformly, it is packed up to the efficient foliage bacterial fertilizer A of tea tree.
Foliar fertilizer B formula composition and preparation, (1) composition：5 parts of chitosan, 0 part of amino acid 1,5 parts of seaweeds, micro- fertile 15
Part, 65 parts of water.(2) it prepares：1. taking chitosan, amino acid, seaweeds and micro- fertilizer to be sufficiently stirred by regulation proportion is dissolved in required match
In the sterile water of ratio, tea free leaven B is made.
2. field trial：
Experimental field overview：Experimental field it is located at Guizhou Zhengan County tea tree garden, kind is " Zhengan County's white tea ", and mean sea level is
1276m, subtropical zone monsoon moist climate, has a moderate climate, and 15.2 DEG C average, abundant rainfall, long annual rainfall is in 1100-1300
Between millimeter, the hydro-thermal same period.It is yellow earth for examination tea garden soil type, random in tea place before test, multiple spot mixing acquisition depth 0-
The soil sample of 60cm is as soil background, pH value 5.86, total nitrogen content 1.72gkg-1, the content of organic matter is
36.58g·kg-1, alkaline hydrolysis nitrogen content is 106.76mgkg-1, available phosphorus content 4.67mgkg-1, effective potassium content is
3.66mg·kg-1, effective iron content 55.36mgkg-1。
Field experimental design：RANDOMIZED BLOCK DESIGN is taken in test, spraying using complete stool (including blade face front and back sides, limb),
If 100 times of 100-200 times of liquid of foliage bacterial fertilizer, foliar fertilizer liquid spray clear water as control (CK), totally 3 processing, are repeated 4 times, totally 12
A cell around sets protection row.Administration time is application tea tree rudiment early period, and amount of application is 45kg/ mus.Weather is equal during test
Based on fine, or it is cloudy, spray in latter 3-4 days without rainfall.
3. analysis project and method：
Yield and quality：Investigation dry tea yield, the young sprout fresh leaf water extraction content of tealeaves, tea polyphenols contains in fresh tea leaves
Free aminoacid content, fresh tea leaves Determination of Chlorophyll total amount in amount, fresh tea leaves.Investigate breeding time tea tree anthracnose, moire blight,
Gall of tea incidence counts cumulative life-incidence；Disease incidence (%)=100 × (morbidity strain number/investigation strain number).
4. embodiment effect：
From table 1 to table 2 as can be seen that can effectively increase tea cured leaf yield, extremely significant raising using tea tree foliage bacterial fertilizer
Fresh leaf water extraction, tea polyphenols, free amino acid and chlorophyll content promote the formation of tea bush productivity and the improvement of quality；And
The generation of the diseases such as tea tree anthracnose, moire blight, gall of tea can be effectively reduced.
Table 1 applies influence of the foliage bacterial fertilizer to tea bush productivity quality
Note：It indicates with different lowercases are marked after column data in P<0.05 lower difference reaches the level of signifiance, different capitalizations
It indicates in P<0.01 lower difference is same up to extremely significant horizontal following table
Table 2 applies foliage bacterial fertilizer, and to diseases and pests of tea tree, a situation arises
It is above specific application example of the invention, there are other embodiments of the invention, all to use equivalent replacement
Or the technical solution that equivalent transformation is formed, it all falls within protection scope of the presently claimed invention.
Claims (6)
1. a kind of efficient foliage bacterial fertilizer of tea tree, it is characterised in that：It is made from the following raw materials in parts by weight：Thermophilic malt oligotrophy unit cell
10-20 parts of bacteria agent, 8-12 parts of providencia stuartii microbial inoculum, 8-12 parts of monospore category bacteria agent, 3-7 parts of chitosan, amino
8-12 parts sour, 3-7 parts of seaweeds, micro- 10-20 parts of fertilizer, 20-40 parts of water.
2. the efficient foliage bacterial fertilizer of tea tree according to claim 1, it is characterised in that：It by following parts by weight raw material system
At：15 parts of germ oligotrophy unit cell microbial inoculum, 10 parts of providencia stuartii microbial inoculum, 10 parts of monospore category bacteria agent, chitosan 5
It is part, 0 part of amino acid 1,5 parts of seaweeds, fertile 15 parts, 30 parts of water micro-.
3. the efficient foliage bacterial fertilizer of tea tree according to claim 1 or 2, it is characterised in that：The germ oligotrophy unit cell
The strain of microbial inoculum is germ oligotrophy unit cell XQ08, the strain of providencia stuartii microbial inoculum is providencia stuartii
JD, monospore category bacteria agent strain be monospore category bacterium J3；The microbial inoculum preparation includes the following steps：(1) by strain X Q08, JD and
The inclined-plane J3 saves test tube seed, is seeded in minimal medium respectively, shaken cultivation to logarithmic growth phase, obtains bacterium solution；(2)
The bacterium solution of acquisition is seeded in the seed fermentation tank containing minimal medium with the inoculum concentration of 10% percent by volume, is trained
It supports to logarithmic growth phase, obtains seed liquor；(3) seed liquor of acquisition is inoculated with according to the inoculum concentration of 10% volume ratio into fermentation
In culture medium, fermented and cultured 2-3 days at 30-35 DEG C, 150-200rpm obtains fermentation liquid, and the living bacteria count of fermentation liquid is equal
Fermentation liquid is directly diluted 5-20 times respectively and obtains general sieve of germ oligotrophy unit cell liquid bacterial agent, Si Shi by >=100 hundred million/mL
Wei Dengsi bacteria liquid microbial inoculum and monospore category bacteria liquid microbial inoculum.
4. the efficient foliage bacterial fertilizer of tea tree according to claim 1 or 2, it is characterised in that：The chitosan is water solubility, is taken off
Acetyl degree is greater than 95%；The amino acid is proline, glycine, glutamic acid, phenylalanine, leucine, isoleucine, relies ammonia
Acid, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, arginine, asparatate,
Any five kinds or more of combination in cysteine；The seaweeds are pure natural marine algae extract；Micro- fertilizer is quality
Than for:3-6:2-5:1-2:1-2:1-2:1-2:0.5-1:0.5-1:Potash fertilizer+calcareous fertilisers+boron fertilizer+fertiliser containing magnesium+iron fertilizer+zinc fertilizer of 0.5-1
+ selenium fertilizer+molydbenum fertilizer+manganese fertilizer mixed fertilizer；The water is sterile water.
5. a kind of preparation method of the efficient foliage bacterial fertilizer of tea tree as claimed in claim 1 or 2, it is characterised in that：Including as follows
Processing step：
Step 1. takes germ oligotrophy unit cell liquid bacterial agent, providencia stuartii described in claim 4 by regulation proportion
Liquid bacterial agent and monospore category bacteria liquid microbial inoculum, it is spare；
Step 2. is matched by regulation takes chitosan, amino acid, seaweeds and micro- fertilizer that the sterile water for being dissolved in required proportion is sufficiently stirred
In；
The microbial inoculum that step 1 obtains is added in the solution that step 2 obtains step 3., the item in 25-30 DEG C of temperature, pH6.0~6.8
Under part, it is sufficiently mixed in sterile blender uniformly, it is packed up to tea tree blade face bacterial fertilizer products.
6. a kind of application of the efficient foliage bacterial fertilizer of tea tree as claimed in claim 1 or 2, it is characterised in that including following side
The application in face：(1) increase the yield of tea tree；(2) improve tea tree quality；(3) induction enhances tea tree disease resistance, and effective prevention and control include
Disease including tea tree anthracnose, moire blight, gall of tea；(4) promote the growth of tea tree plant.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810861230.5A CN108840746A (en) | 2018-08-01 | 2018-08-01 | A kind of efficient foliage bacterial fertilizer of tea tree and the preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112851446A (en) * | 2021-01-28 | 2021-05-28 | 于佳骏 | Foliar fertilizer for increasing large leaf yield of Pu' er tea trees and preparation method thereof |
-
2018
- 2018-08-01 CN CN201810861230.5A patent/CN108840746A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112851446A (en) * | 2021-01-28 | 2021-05-28 | 于佳骏 | Foliar fertilizer for increasing large leaf yield of Pu' er tea trees and preparation method thereof |
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