CN110367282B - Biological stimulator compound agent suitable for cotton and preparation method and application thereof - Google Patents
Biological stimulator compound agent suitable for cotton and preparation method and application thereof Download PDFInfo
- Publication number
- CN110367282B CN110367282B CN201910763188.8A CN201910763188A CN110367282B CN 110367282 B CN110367282 B CN 110367282B CN 201910763188 A CN201910763188 A CN 201910763188A CN 110367282 B CN110367282 B CN 110367282B
- Authority
- CN
- China
- Prior art keywords
- cotton
- compound agent
- biological
- growth
- spraying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/50—Cotton
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/27—Dispersions, e.g. suspensions or emulsions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Dispersion Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a biological stimulator compound agent suitable for cotton and a preparation method and application thereof. The biological stimulator compound agent consists of amino acid, polyglutamic acid, potassium humate and water, and the preparation steps are as follows: 1) preparing polyglutamic acid fermentation liquor by fermentation, and then performing ultraviolet inactivation; 2) preparing potassium humate which can be dissolved in water; 3) adding amino acid powder into polyglutamic acid solution; 4) adding the prepared potassium humate into the solution in the step 3, and emulsifying by an ultrasonic oscillator to form the suspended biological stimulant compound agent. The biological stimulator compound agent is sprayed on the leaf surface of the seedling stage for 1-2 times, so that the seedlings can be protected and strengthened; the leaf surface is sprayed for 1-2 times in the bud period, and bud formation can be promoted; the foliage is sprayed for 1-2 times in the flowering period, so that flower bud falling can be reduced; the cotton boll growth can be promoted and the single boll weight can be increased by spraying the foliage for 2-3 times in the boll period; the full-growth foliage spraying for 3-4 times can promote the growth of cotton plant, raise the resistance, regulate the balance between vegetative growth and reproductive growth, raise the boll forming number of single plant and raise yield.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a biological stimulant compound agent suitable for cotton, a preparation method of the biological stimulant compound agent suitable for the cotton, and application of the biological stimulant compound agent to the cotton.
Background
The biostimulant is a general name of various substances and microorganisms capable of promoting plant growth, and mainly comprises the following components: microbial inoculants, humic acid, fulvic acid, chitin and chitosan derivatives, protein hydrolysates, amino acids, seaweed extracts and the like; the application of the biological stimulator is wide on fruits and vegetables, and the market value of the global biological stimulator products in recent years is increased at the speed of 3-6 hundred million dollars per year on average, please see: pamela Calvo, Louise Nelson, Joseph w.kloepper.agricultural uses of Plant biological [ J ]. Plant and Soil, 2014, 33: 3 to 41; salix purpurea, lujiang.sudoku effect of biostimulin in agriculture [ J ] popularization of Qinghai agricultural technology, 2015, 2: 55-56; liuxiuxiu, von ludongpo, biological stimulin in agriculture, current application and development prospects [ J ] southern agriculture, 2017, 11 (4): 88-89.
Research shows that humic acid can raise plant root system H+The activity of ATP enzyme and the like, promotes the growth of plant root systems, activates phosphate and potassium ions in soil, increases the absorption of plants to nutrients, and can regulate metabolism in plants so as to promote the growth of plants, see: zhangshui, Yuan Lian, Lin Zhi an, Li Yan Ting, Hu Tree character, Zhao inheres the strength, humic acid promotes the mechanism research progress of plant growth [ J]Plant nutrition and fertilizer academy, 2017, 23 (4): 1065-1076. It is also reported that the foliar application of the biological stimulin can promote the germination of the greenhouse large cherries, advance flowering, increase the yield and improve the quality, please see: application test of biological stimulin in greenhouse large cherry (J)]Northern fruit tree, 2016, 6: 12-13. The biological stimulin applied to the soil can promote the growth of rhizobia of soybean, increase the content of rhizobia in rhizosphere soil microorganisms and promote the growth and development of soybean, please see that: influence of biostimulant on soybean rhizosphere soil microbial community [ J]Soybean science, 2017, 36 (5): 751-760.
Polyglutamic acid (gamma-PGA), also called natto gum and polyglutamic acid, is a biological polymer which is water-soluble, biodegradable and nontoxic and is prepared by using a microbial fermentation method. It can make crops more effectively absorb phosphorus, calcium, magnesium and trace elements in soil.
Cotton is used as an important field economic crop, is planted in the northwest inland area, the yellow river basin and the Yangtze river basin in China, and please see that: wanseo, division and transition of chinese cotton district, 2009 (9): 12-16. This shows that cotton has wide ecological adaptability, but the environment and climate of different ecological areas are different, and the growth and development time of cotton is different. Unlike other crops, the core product of cotton is cotton fiber, which is developed by epidermal cells bred on cotton seeds and is neither seeds nor fruits. In addition, due to the long growth and development period of cotton and the strong competition between the vegetative growth and the reproductive growth of cotton, cotton boll shedding and greedy late maturity are caused by the over-vigorous vegetative growth, and the yield is reduced.
Stress causes secondary oxidative stress, increases the level of active oxygen in plants, accumulates excessive active oxygen such as hydrogen peroxide, superoxide anion, hydroxyl radical, and causes protein, DNA and lipid peroxidation damage.
Such as reducing the respiration efficiency of plants under the condition of saline-alkali stress, uncoupling the electron transfer and oxidative phosphorylation, synthesizing stress protein and organic osmotic substance in plants at the early stage of the stress, accumulating or rejecting salt ions to repair damages, and Na in the soil with saline-alkali stress+、Cl-And Mg+The plasma concentration is too high, so that the nutrient element K is generated+、NO3 -、Ca+The decrease in absorption causes the plants to be asymptomatic, and many enzymes of plant cells are active only in a narrow range of ion concentration, while Na is present+、Cl-And Mg+Plasma enters cells to enable protoplasm to be condensed, chlorophyll is destroyed, protein synthesis is inhibited, proteolysis is enhanced, amino acid accumulation in vivo is caused, partial amino acid is converted into butanediamine, pentanediamine and free ammonia, the cells are poisoned and die when reaching a certain concentration, decomposition of nucleic acid is larger than synthesis under saline-alkali stress, amino acid synthesis is hindered, most of the synthetic protein is salt-tolerant protein, and sugar, amino acid and other small molecular substances decomposed from starch and protein are stabilized on cell membranes, protein structures and protoplasts to reduce osmotic potential of the cells under the salt stress, so that plant cells are prevented from being injured or are lightened; these factors often lead to cotton boll shedding.
Under drought stress, phospholipid molecules in membranes of cell membrane systems are disorderly arranged, lipophilic ends attract each other to form empty attacks, membrane proteins are damaged, the selective permeability of the membranes is changed, a large number of small molecules such as inorganic ions, amino acids and soluble sugars leak out of tissues, under the condition of water deficiency, the activity of ribonuclease is increased along with the water deficiency, the decomposition of RNA and mRNA is accelerated, and polyribosomes are decomposed into subunits or mononucleosomes, so that the synthesis of proteins is inhibited, meanwhile, the content of Catalase (CAT), Peroxidase (POD), Glutathione Reductase (GR) and superoxide dismutase (SOD) is reduced, membrane damage is accelerated, young leaves can also take water from old leaves, flower buds or fruits, and flower and fruit drop is caused.
Under the stress of low temperature, the membrane system of plant cells is the most sensitive part, at the moment, the phase change of cell membrane substances occurs, the membrane permeability is increased, a large amount of electrolyte leaks, the relative conductivity of cytoplasm is increased, the serious damage of a cell enzyme protection system is caused, the concentration of amino acid, particularly free amino acid can be increased, the proportion of non-freezing water in tissues is improved, and the positive relation is formed between the amino acid, particularly the free amino acid, and the cold resistance of plants.
The cotton is a relatively salt-tolerant crop, but the salt tolerance of the cotton is not more than 0.3 percent, the growth of the cotton is severely inhibited under the condition of salt and alkali stress (Mass and Hoffman, 1977), the limitation of high salt stress and drought stress on the yield of the cotton still causes the primary problem to be solved, the functions of the prior art (bio-stimulin) are growth promotion, the preparation technology of the bio-stimulants is developed around a single bio-stimulants, the bio-stimulants suitable for the cotton application and the preparation promotion and application methods cannot be clearly provided, and the technical problem of how to apply the bio-stimulants for improving the growth of cotton plants, improving the stress resistance and coordinating the vegetative growth and the reproductive growth in production cannot be answered. If the IAA is sprayed to promote the growth of the plants, the plants absorb a large amount of water to avoid the increase of the salt concentration in the body; the ABA can induce stomata to close, thereby reducing transpiration, reducing passive absorption of salt and improving the salt resistance of crops; salicylic acid and jasmonic acid substances can change the content of osmotic substances in plants to increase drought resistance, salt resistance and stress caused by temperature as signal substances; chitosan can protect plasma membrane damage of plants under stress and alleviate peroxidation damage.
Disclosure of Invention
The invention aims to provide a biological stimulator compound agent suitable for cotton, a preparation method thereof and application of the biological stimulator compound agent in cotton production, the compound agent has unique formula, reasonable material composition, easy preparation method and convenient use, can be applied in each stage of cotton growth, can effectively promote the growth of cotton plants in the early stage, has the capability of regulating the balance between later-stage vegetative growth and reproductive growth, improves the adaptability of the cotton plants in a adverse environment, reduces the shedding of flower buds and bolls, improves the boll forming number of a single plant and improves the yield.
In order to achieve the purpose, the invention adopts the following technical scheme:
a bio-stimulant compound agent suitable for cotton is prepared from the following raw materials in percentage by mass:
the polyglutamic acid fermentation liquor contains 10-15 wt% of polyglutamic acid.
A bio-stimulant compound agent suitable for cotton is prepared from the following raw materials in percentage by mass:
a bio-stimulant compound agent suitable for cotton is prepared from the following raw materials in percentage by mass:
a bio-stimulant compound agent suitable for cotton is prepared from the following raw materials in percentage by mass:
a method for preparing a bio-stimulant compound agent suitable for cotton comprises the following steps:
A. preparing a polyglutamic acid fermentation liquid containing 10-15% of polyglutamic acid by microbial fermentation, and performing ultraviolet inactivation on microorganisms after the fermentation is finished to obtain a polyglutamic acid solution;
B. reacting mineral humic acid with potassium hydroxide or potassium oxide or potassium carbonate to prepare potassium humate which can be dissolved in water, weighing the potassium humate according to a proportion, and dissolving the potassium humate in boiling water to obtain a potassium humate solution;
C. weighing amino acid powder and polyglutamic acid fermentation liquor according to a proportion, mixing, stirring and dissolving to obtain an amino acid mixed solution;
D. and C, completely adding the prepared potassium humate solution in the step B into the amino acid mixed solution in the step C, and emulsifying by using an ultrasonic oscillator at 35 ℃ and 60KHz frequency to form a suspended preparation, namely the biological stimulant compound agent suitable for cotton.
The application of the biological stimulator compound agent suitable for cotton in increasing the yield of cotton comprises the following steps of spraying in any one of the following growth periods:
a. spraying the mixture for 1-2 times according to the mass ratio of 1:800 from the seedling emergence to the bud stage, wherein the water consumption per mu of cotton field is 15kg, the dosage per mu of the biological stimulant compound agent is 18.75g, and the spraying interval between two times is 5-7 days;
b. spraying the biological stimulin compound agent for 1-2 times according to the mass ratio of 1:800 from the bud period to the flower bell period, namely, 30kg of water consumption per mu of cotton field, 37.5g of the biological stimulin compound agent per mu of cotton field, and 5-7 days of interval between two spraying;
c. and (3) spraying the biological stimulin compound agent for 2-3 times in the boll period according to the mass ratio of 1:1200, wherein the water consumption of each mu of cotton field is 30kg, the dosage of each mu of the biological stimulin compound agent is 25g, and the interval between two times of spraying is 7-9 days.
Compared with the prior art, the invention has the following advantages and effects:
through the measures, the applicant obtains a technical means of applying the targeted biological stimulator mixture on cotton, and the biological stimulator mixture with a certain concentration is applied externally in the seedling stage, the bud stage, the flowering stage and the boll stage of the cotton to regulate the relationship between the vegetative growth and the reproductive growth of cotton plants, promote the transformation of flower buds to cotton bolls, reduce the falling, improve the boll forming number of a single plant and improve the yield; promoting the growth and development of weaker cotton plants or cotton plants under the influence of low temperature, drought and high temperature, improving the stress resistance of the cotton plants, reducing falling, improving the boll forming number of single plants and ensuring the yield, thereby improving the comprehensive benefit of cotton planting and ensuring the high-efficiency production of cotton. The field test result shows that the biological stimulator compound agent with a certain concentration can obviously promote the reproductive development of cotton, increase the boll forming number of a single plant by 1-3, and obviously increase the yield, wherein the yield increase rate in 2016 is 11.0-12.7%, and the yield increase rate in 2017 is 15.6-17.5%.
The biological stimulator compound agent has the functions of activating phosphate and potassium ions in soil, enhancing the absorption of trace elements by plants and the like and promoting the growth of cotton plants, thereby increasing the yield of cotton. Because the potassium humate in the biological stimulant compound agent is in an activated state, the amino acid belongs to small molecular substances, the substances can be efficiently absorbed by cotton leaves under the condition of spraying on the leaves, and the advantages of the absorption of the leaves are that the metabolism and the transportation are convenient, nutrients are directly delivered to organs with vigorous growth and development, so that the cotton seedlings (1 leaf phase in Xinjiang and 1 leaf phase in the yellow river basin and 3 leaf phase in the yellow river basin) are promoted to resist the early adverse circumstances (the yellow river basin, the salt and the alkali in the cotton area in Xinjiang and the low night temperature), the supplemented nutrient substances improve the influences of reduction of the activity of the cotton root system, the absorption antagonism of mineral nutrient ions of the root system, the accumulation of hydrogen peroxide, the transportation and the accumulation reduction of photosynthetic products and the like caused by the drought, the salt and the alkali and the low night temperature, and proline in the amino acid additive can protect the plasma membrane of plants under stress and reduce the peroxide injury.
The effect of promoting and strengthening the seedlings is achieved; and the amino acid and other small molecular compounds in the bio-stimulant compound are used as metabolites in plants, so that the bio-stimulant compound is not only a basic raw material for substance synthesis in plants, but also a substance signal can further condition the growth and metabolism of cotton, the relationship between reproductive growth and vegetative growth can be adjusted, the transformation from vegetative growth to reproductive growth in the flowering and boll-forming period is promoted, more photosynthetic nutrients are stored in bolls, the bolls are prevented from falling off due to insufficient nutrition, the boll forming number of a single cotton plant is increased, and the higher yield is obtained.
In addition, substances related to the invention are not simply superposed, but are compounded into a preparation capable of being efficiently absorbed by cotton according to the characteristics of a wax layer of a cotton leaf and the molecular particle size of raw materials; the carbon-nitrogen ratio of the total amount of the substances, the ratio of activated humic acid to amino acid in the active substances, and the ratio of polymerized amino acid to common amino acid in the invention are determined according to the physiological and metabolic characteristics of cotton, which is a crop in the growth and development period.
The rice plant externally applied with the r-polyglutamic acid has higher antioxidant enzyme activity, less accumulated ROS, more osmoregulation substances such as proline, soluble sugar and the like, and the more balanced ion proportion and distribution of the rice are kept; gamma-polyglutamic acid is a high molecular substance which cannot pass through the membrane structure and can cause the change of some substances on the cell membrane. The potassium fulvate and the gamma-polyglutamic acid have synergistic effect in the aspects of increasing the activity of antioxidant enzyme in cotton plants and regulating substances by osmosis.
Detailed Description
In order to make the objects, contents, and advantages of the present invention clearer, the following detailed description is given with reference to examples.
Example 1:
a bio-stimulant compound agent suitable for cotton is prepared from the following raw materials in percentage by mass:
the polyglutamic acid fermentation liquor contains 10-15 wt% of polyglutamic acid.
A method for preparing a bio-stimulant compound agent suitable for cotton comprises the following steps:
A. preparing 10-15% of polyglutamic acid fermentation liquor (the concentration of polyglutamic acid is 10-15%) by microbial fermentation, and performing ultraviolet inactivation on the microbes after the fermentation is finished to obtain polyglutamic acid solution;
B. reacting mineral humic acid with potassium hydroxide or potassium oxide or potassium carbonate to prepare potassium humate which can be dissolved in water, and dissolving 22kg of prepared potassium humate in 15kg of boiling water to obtain a potassium humate solution;
C. adding 18kg of amino acid powder (containing one or more of glycine, proline, lysine and the like) into 45kg of polyglutamic acid fermentation liquor solution obtained in the step A, and stirring and dissolving to obtain a solution;
D. and (4) completely adding the prepared potassium humate solution in the step (B) into the solution in the step (C), and emulsifying by using an ultrasonic oscillator at 35 ℃ and 60KHz frequency to form a suspended preparation, namely the biological stimulin compound agent suitable for cotton with the total mass of 100 kg.
The biological stimulator compound agent suitable for cotton provided by the embodiment has a unique formula, reasonable material composition and a simple and convenient preparation method; the raw materials are reasonable in proportion, and the properties of the components are stable; the environment is friendly, the ecology is safe, and the problem of environmental pollution is avoided; is easy to be popularized and applied.
Firstly, the compound preparation of the embodiment has the function of a single substance, and the composition of the compounded substance is more reasonable, is more beneficial to absorption and utilization of cotton, and shows the function. Secondly, the compound preparation provided by the embodiment is water-based, and is convenient for various application modes such as field spraying, flushing application, drip irrigation and the like. And thirdly, the prepared potassium humate solution is activated by boiling water and is more beneficial to plant absorption and utilization, the polyglutamic acid fermentation liquor contains certain polyglutamic acid and various active substances such as sugar, amino acid, auxin and the like metabolized by microorganisms, the measures are simple and convenient through ultraviolet inactivation, and the influence on the physicochemical property of the compound agent caused by the continuous activity of the microorganisms in the solution is avoided.
Example 2 to example 7:
a biological stimulator compound agent suitable for cotton is prepared from the following raw materials in percentage by mass (%):
TABLE 1
The procedure was the same as in example 1. The polyglutamic acid fermentation liquor contains 10-15 wt% of polyglutamic acid.
Examples 2 to 7 are series of formulas prepared for the growth characteristics of cotton in different cotton production areas and different planting modes (direct seeding or transplanting) and at different growth and development stages of cotton, respectively.
The biological stimulin compound prepared by the method can be used for spraying cotton plant leaf surfaces after being diluted by a certain multiple, and the application amount of each component per mu is shown in the following table 2
The actual usage amount (g/acre) of amino acids, polyglutamic acid, and potassium humate per acre in example 1 as in table 2 above was calculated as follows:
diluted according to the mass ratio of 1:800, then 800g of water is needed to be added into 1g of the compound agent.
The amount of water used in example 1 was 15L, i.e., 15 kg.
According to the formula 1:800 ×: 15kg
The dose of the compounded agent is 1 × 15kg ÷ 800 ÷ 0.01875kg ═ 18.75g
According to the embodiment 1, the amino acid accounts for 18 percent, the polyglutamic acid fermentation liquor (the content of the polyglutamic acid is 10-15 percent) accounts for 45 percent, and the potassium humate accounts for 22 percent;
therefore, on the premise that the water consumption is 15kg per mu, the compound agent is applied according to the mass ratio of 1:800, the using amount of the compound agent is 18.75g, and the application amount per mu is further calculated as follows: amino acids (18.75g × 18%: 3.38g), polyglutamic acids (18.75g × 45% × 10%: 0.84g, 18.75g × 45% × 15%: 0.1.27g), and potassium humate (18.75g × 22%: 4.13 g).
Example 8:
the application of the biological stimulator compound agent suitable for cotton in the cotton seedling stage of the cotton area in the yellow river basin comprises the following steps:
in 2016, in a mature cotton field in the yellow river basin, a field plot test is adopted, 5 treatments are set, each treatment is repeated for 4 times, and each test plot is 20m2. The test is carried out on the leaf surface spraying in the 3-leaf period, 5 test cells respectively adopt the biological stimulin compound agent (diluted by 800 times for application), the potassium fulvate (test concentration is 0.50g/L), the amino acid (application concentration is 0.33g/L), the polyglutamic acid (application concentration is 0.10g/L) and the clear water in the example 2, the spraying is carried out for 2 times, the interval is 7 days each time, the water consumption is 15kg for each mu of spraying, other field management modes are consistent, the sampling is carried out after the spraying is carried out for 15 days for the first time, the plant height, the stem thickness and the leaf area of cotton plants are measured, and the dry matter is measured, and the results are shown in a table 3.
The test result shows that compared with the clear water control, the application of the amino acid, potassium fulvate, polyglutamic acid and the biological stimulator compound agent (example 2) with certain concentration can improve the stem thickness, the plant height, the leaf area and the total dry weight of the cotton plant, but the action effect of example 2 is obviously higher than that of the single application of the potassium fulvate, the amino acid and the polyglutamic acid. Therefore, the biological stimulator compound agent (example 2) with a certain concentration can be definitely applied to obviously promote the growth and development of cotton seedlings and establish a good basic framework for budding and bolling in the later period.
TABLE 3 Effect of cotton in seedling stage after application of biostimulants
Example 9:
the application of the biological stimulator compound agent suitable for cotton in the cotton seedling stage of Xinjiang cotton area comprises the following steps:
in 2017, in cotton fields in Aksu area of southern Xinjiang, field plot experiments are adopted, 5 treatments are set, each treatment is repeated for 3 times, and each experimentTest cell 45.6m2. The leaf surface spraying is carried out in the 1-leaf one-heart period in the experiment, 5 experimental cells respectively adopt the biological stimulin compound agent (diluted by 800 times for application), the potassium fulvate (the experimental concentration is 0.50g/L), the polyglutamic acid (the application concentration is 0.10g/L), the amino acid (the application concentration is 0.33g/L) and the clear water in the embodiment 6, the biological stimulin compound agent is sprayed for 2 times, the water consumption is 15kg for spraying every mu at intervals of 7 days, other field management modes are consistent, the sampling is carried out after the spraying for 15 days for the first time, the dry weight of cotton plant roots and the dry weight of overground parts are measured, and the total dry matter is measured, and the results are shown in a table 4.
The test results show that, compared with the clear water control, the compound agent (example 6) containing polyglutamic acid, potassium fulvate, amino acid and biological stimulin with a certain concentration can obviously improve the dry weight of cotton plant roots, the dry weight of overground parts and the total dry weight. And the action effect of the embodiment 6 is obviously higher than that of the single application of the polyglutamic acid, the potassium fulvate and the amino acid. Therefore, the biological stimulator compound agent with a certain concentration (example 6) can be definitely applied to remarkably promote the growth and development of cotton in seedling stage of Xinjiang cotton area, improve the capability of cotton plants to resist the adverse circumstances such as saline-alkali stress, drought stress and the like, and lay a foundation for the growth and boll formation of later-stage cotton.
TABLE 4 Effect of cotton in seedling stage after application of biostimulants
Example 10:
the application of a biostimulant in cotton boll stage is as follows:
2016 and 2017, in a cotton field of the yellow river valley, adopting a field plot experiment, setting 8 experiment plots, respectively adopting one of the following biological stimulin solutions to carry out foliage spraying according to the table 5 on cotton plants in seedling stages in the 8 experiment plots, wherein other management modes are consistent, each experiment plot is repeatedly sprayed for 3 times, the interval is 7 days every time, and each experiment plot is 64m2Spraying 30kg of water per mu, investigating and sampling at a mature period after spraying the leaf surfaces, and measuring the dry matter and yield of cotton plants.
Test results show that compared with clear water control, the application of potassium fulvate (test concentration of 0.50g/L), amino acid (application concentration of 0.33g/L), polyglutamic acid (application concentration of 0.10g/L) and the biological stimulator compound agent (examples 3, 5 and 7) can obviously increase the dry matter of cotton plants, the boll forming number of single plants and the yield; and the application of the biological stimulator compound agent with a certain concentration (examples 3, 5 and 7, diluted by 1200 times) has obvious better effect than the application of potassium fulvate, amino acid and polyglutamic acid with a certain concentration. Compared with clear water, the biological stimulants compound agent (examples 3, 5 and 7) is applied, the increase rate of the single plant boll forming number in 2016 years is 9.8-13.7%, the yield increase rate per mu is 11.0-12.7%, the increase rate of the single plant boll forming number in 2017 is 15.6-17.8%, and the yield increase rate is 15.6-17.5%.
TABLE 5 Effect of biostimulant application in boll stage of Cotton
Claims (4)
1. The application of the biological stimulant compound agent suitable for cotton in increasing the yield of cotton is characterized in that the biological stimulant compound agent is prepared from the following raw materials in percentage by mass:
the polyglutamic acid fermentation liquor contains 10-15 wt% of polyglutamic acid,
the biological stimulator compound agent is sprayed in any one of the following growth periods:
a. spraying the mixture for 1-2 times according to the mass ratio of 1:800 from the seedling emergence to the bud stage, wherein the water consumption per mu of cotton field is 15kg, the dosage per mu of the biological stimulant compound agent is 18.75g, and the spraying interval between two times is 5-7 days;
b. spraying the biological stimulin compound agent for 1-2 times according to the mass ratio of 1:800 from the bud period to the flower bell period, namely, 30kg of water consumption per mu of cotton field, 37.5g of the biological stimulin compound agent per mu of cotton field, and 5-7 days of interval between two spraying;
c. and (3) spraying the biological stimulin compound agent for 2-3 times in the boll period according to the mass ratio of 1:1200, wherein the water consumption of each mu of cotton field is 30kg, the dosage of each mu of the biological stimulin compound agent is 25g, and the interval between two times of spraying is 7-9 days.
2. The use of a bio-stimulant compound for cotton according to claim 1 for increasing cotton production, wherein:
3. the use of a bio-stimulant compound for cotton according to claim 1 for increasing cotton production, wherein:
4. the use of a bio-stimulant compound for cotton according to claim 1 for increasing cotton production, wherein:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910763188.8A CN110367282B (en) | 2019-08-20 | 2019-08-20 | Biological stimulator compound agent suitable for cotton and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910763188.8A CN110367282B (en) | 2019-08-20 | 2019-08-20 | Biological stimulator compound agent suitable for cotton and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110367282A CN110367282A (en) | 2019-10-25 |
| CN110367282B true CN110367282B (en) | 2021-12-03 |
Family
ID=68259863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910763188.8A Active CN110367282B (en) | 2019-08-20 | 2019-08-20 | Biological stimulator compound agent suitable for cotton and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110367282B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990412B (en) * | 2020-07-21 | 2021-09-03 | 广东拉多美化肥有限公司 | Humic acid composite plant high-temperature-resistant protective agent and preparation method thereof |
| CN112552107A (en) * | 2020-12-10 | 2021-03-26 | 宁夏伊品生物科技股份有限公司 | Organic fertilizer containing gamma-polyglutamic acid, preparation method and application thereof |
| CN112661581A (en) * | 2021-01-05 | 2021-04-16 | 深圳市芭田生态工程股份有限公司 | Anti-sunburn biological source stimulant, crop fertilizer and crop irrigation and spraying method |
| CN114524692A (en) * | 2022-01-21 | 2022-05-24 | 沃达农业科技股份有限公司 | Control promoting pesticide fertilizer for cotton seedling-stage drip irrigation and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104761411A (en) * | 2015-03-21 | 2015-07-08 | 史丹利化肥股份有限公司 | Garlic fertilizer by tower-type melt granulation and preparation method hereof |
| CN107641045A (en) * | 2016-07-20 | 2018-01-30 | 南京轩凯生物科技有限公司 | A kind of composite synergist and its application containing polyglutamic acid and potassium fulvate |
| CN108863555A (en) * | 2018-07-27 | 2018-11-23 | 中国农业科学院棉花研究所 | A kind of liquid-type foliar fertilizer synergist and preparation and application |
-
2019
- 2019-08-20 CN CN201910763188.8A patent/CN110367282B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110367282A (en) | 2019-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110367282B (en) | Biological stimulator compound agent suitable for cotton and preparation method and application thereof | |
| CN103396232B (en) | Biological fertilizer | |
| CN103980052B (en) | One special fertilizer for fruit trees and preparation method thereof | |
| CN108849984B (en) | Wheat drought-tolerance stress-tolerance compound regulator and application thereof | |
| CN102167634B (en) | Oligosaccharide-containing foliar fertilizer composite and application thereof | |
| CN108440146A (en) | A kind of full trophic function type suspension liquid fertilizer of high concentration and preparation method thereof | |
| CN102584448B (en) | Compound fertilizer | |
| CN105123759B (en) | Saline alkali tolerant plant growth promoter | |
| CN102701861A (en) | Plant nutrient liquid fertilizer | |
| CN108218597A (en) | A kind of multiple-element long active fruit-specific fertilizer and preparation method thereof | |
| CN103304317B (en) | Humic acid liquid fertilizer | |
| CN102703088A (en) | Saline-alkali soil improved medicament and saline-alkali soil improved afforestation and soil preparation method | |
| CN104926534A (en) | High-purity chito-oligosaccharide controlled release fertilizer based on separation techniques and preparing method thereof | |
| CN108271787A (en) | A kind of compound oligosaccharide fruits and vegetables support the preparation method of root element | |
| CN102531800A (en) | Agricultural-residue-free biodegradation technology | |
| CN102260117B (en) | High-efficiency compound foliar fertilizer | |
| CN101774851A (en) | Microorganism-containing organic compound fertilizer for stevia rebaudiana | |
| CN115124376A (en) | Compound biological fertilizer and preparation method thereof | |
| CN111943764A (en) | Agricultural soil-modified nano-calcium foliar fertilizer and fertilizing method thereof | |
| CN107244992A (en) | A kind of liquid biological organic fertilizer and preparation method thereof | |
| CN106866287A (en) | A kind of rich magnesium nutrition for promoting root crop growth is plain | |
| CN113929532A (en) | Brown algae oligosaccharide water-soluble fertilizer and preparation method thereof | |
| CN101223848B (en) | Use of poly-gamma-glutamate in burley tobacco field production | |
| CN104446858A (en) | Humic acid water-soluble fertilizer | |
| CN107827520A (en) | A kind of change controlling agent for improving wheat yield and change prosecutor method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |