Summary of the invention
The object of the invention is, strengthen the activity of callus cell, promote the cell division of stock and scion, accelerate the speed of callus healing.
A kind of grafting inorganic agent, comprise the raw material composition of following weight portion: 6-benzyl aminopurine 35-50 part, zeatin 24-45 part, phosphatid ylcholine 15-30 part, carbonic hydroammonium 5-15 part, asparatate 0.001-0.01 part, shitosan 10-20 part, pollen pini 5-20 part, diatom powder 5-10 part.
More preferably comprise the raw material composition of following weight portion: 6-benzyl aminopurine 40 parts, zeatin 30 parts, phosphatid ylcholine 20 parts, 10 parts, carbonic hydroammonium, asparatate 0.0035 part, shitosan 14 parts, pollen pini 14 parts, diatom powder 5 parts.
The present invention is by the above-mentioned application of grafting inorganic agent on solanaceous crops graft stock.
Adopt the method for grafting inorganic agent graft stock, comprise the steps:
By 6-benzyl aminopurine, zeatin, phosphatid ylcholine, carbonic hydroammonium, asparatate, shitosan, pollen pini, diatom powder and distilled water are after 1:10 dissolves in mass ratio, and sterilizing 30-50min at 100-120 DEG C, obtains grafting inorganic agent;
The scion of solanaceous crops is immersed above-mentioned grafting inorganic agent 1-3min, insert in the close stock otch of thickness, after alignment, clip by fixing, put on nursery stock bed subsequently;
Control temperature be 25-30 DEG C, under humidity is 30-40%, visible ray illumination every day 8-10h, growth can obtain solanaceous crops graft stock.
Technical scheme of the present invention improves the survival rate in grafting procedures, and callus healing time shortens.
Pollen pini of the present invention, diatom powder are component, and pollen pini, diatom powder have disinfection, anti-mildew effect, can extend the lasting period of medicament of the present invention.
Asparatate of the present invention, shitosan can increase the denseness of mixed liquor, while providing desired nutritional, promotes the growth of grafting crop.
Embodiment
embodiment 1
1,6-benzyl aminopurine 4kg, zeatin 3kg, phosphatid ylcholine 2kg, sodium bicarbonate 1kg, asparatate 0.00035kg is taken with electronic scale, shitosan 1.4kg, pollen pini 1.4kg, diatom powder 0.5kg.
2, get 100ml distilled water and dissolve the above-mentioned material weighed up, be settled to 1L.
3, the solvent of packing 1L, obtains finished product after high-temperature sterilization cooling.
4, during grafting, scion place is soaked 1 ~ 3 minute.
Experimental example (for grafting guinea squash):
1. select stock that stem thickness is close and scion pairing, scion to be first immersed in inorganic agent 1 ~ 3 minute, then to insert in stock otch, after alignment, clip by fixing, be put into seedbed subsequently on the ground.Such grafting 500 strain is experimental group A.
2. select stock and scion pairing that stem thickness is close, scion is inserted in stock otch, after alignment, clip by fixing, be put into seedbed on the ground.Such grafting 500 strain is control group B.
3. note the control of temperature, humidity, illumination, observe the time of scion leaf restoration ecosystem.
4. experimental result is as follows:
|
The time of blade restoration ecosystem |
Survival rate |
A group |
5th day |
99% |
B group |
8th day |
92% |
Show through result of the test: the process solvent of stock can accelerate the speed of callus healing, time shorten about 37.5%, and survival rate improves about 7%, and productivity effect significantly improves.
embodiment 2
1,6-benzyl aminopurine 3.5kg, zeatin 2.7kg, phosphatid ylcholine 1.6kg, sodium bicarbonate 0.58kg, asparatate 0.0005kg is taken with electronic scale, shitosan 2.0kg, pollen pini 2.0kg, diatom powder 0.8kg.
2, get 100ml distilled water and dissolve the above-mentioned material weighed up, be settled to 1L.
3, the solvent of packing 1L, obtains finished product after high-temperature sterilization cooling.
4, during grafting, scion place is soaked 1 ~ 3 minute.
Experimental example (for grafting guinea squash):
1. select stock that stem thickness is close and scion pairing, scion to be first immersed in inorganic agent 1 ~ 3 minute, then to insert in stock otch, after alignment, clip by fixing, be put into seedbed subsequently on the ground.Such grafting 500 strain is experimental group A.
2. select stock and scion pairing that stem thickness is close, scion is inserted in stock otch, after alignment, clip by fixing, be put into seedbed on the ground.Such grafting 500 strain is control group B.
3. note the control of temperature, humidity, illumination, observe the time of scion leaf restoration ecosystem.
4. experimental result is as follows:
|
The time of blade restoration ecosystem |
Survival rate |
A group |
6th day |
98.5% |
B group |
8th day |
92% |
Show through result of the test: the process solvent of stock can accelerate the speed of callus healing, time shorten about 25%, and survival rate improves about 6.5%, and productivity effect significantly improves.