CN101699956A - Method for tender branch cutting propagation of Linze jujube - Google Patents
Method for tender branch cutting propagation of Linze jujube Download PDFInfo
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Abstract
The invention relates to a method for tender branch cutting propagation of a Linze jujube, comprising the following steps of: selecting tender branches, wherein the selected tender branches are the tender branches grown from jujube saplings with semi-lignification under high temperature; collecting the tender branches, soaking tender branch cuttings in a well-prepared mixed solution; constructing a seedbed through the steps of sanding, disinfecting, heat preserving, light and moisture regulating and sterilization; and then carrying out cutting propagation and acclimatization. The invention is a duplicating jujube plantlet method by using current-grown tender branches as propagation materials. The duplication process is actually a root getting process, by which rootless branches are grown into rooting plants after being soaked in auxin and an auxin synergist solution. Compared with the most advanced traditional tissue-cultured seedling method, the invention has breakthroughs on two aspects of: 1, capability of directly taking self-rooted seedlings, which solves the problem of hard root-taking of jujube seedlings; and 2, propagation of pure and high-quality plantlets, which solves the bottleneck problem of propagating the pure and high-quality seedlings in the appropriate technology field through the ages.
Description
Technical field
The invention belongs to forestry seedling breeding technology, is a kind of technology that is specifically designed to the jujube tree breeding specifically.
Background technology
The seeds that jujube tree takes root for the cuttage difficulty, the conventional seedbed system technology obtains the root turion seedling for the plant division of being completely cured, and the nursery stock quantity that this method obtains is few, of poor quality, and planting survival rate is low.Fast development along with Modern Forestry, the quality and quantity of nursery stock with " bottleneck " of restriction jujube industrialized development, in order effectively to solve this " bottleneck ", scientific and technical personnel successively adopt root turion budling Gui Pu to grow seedlings, stem-tip tissue is cultivated and to be grown seedlings and test such as graft seedling growth, these achievements in research have obtained extensive use on producing, effectively promoted breeding of jujube tree nursery stock, in short supplyly played certain effect to what alleviate nursery stock, but above-mentioned seedling-cultivating method respectively has pluses and minuses, its limitation is also respectively arranged, therefore, " bottleneck " problem of nursery stock still exists: traditional plant division of being completely cured nursery stock quantity that obtains of growing seedlings is few, of poor quality, survival rate is low after the field planting, poor growth; The root turion budling Gui Pu nursery stock that obtains of growing seedlings makes moderate progress than the seedling quality that traditional offshoot of being completely cured obtains, but the nursery stock that obtains with group training seedling method specific mass is obviously not good enough mutually, and the root turion budling gathers difficulty, can't breed in a large number; The seedling root prosperity that the way of employing group cultivation seedling obtains, the planting survival rates height, it is vigorous to grow, resistance has tangible quantity and quality-advantage, but group is cultivated the equipment complexity that seedling needs, operating technology requires high, need to consume great amount of manpower, electric power and chemicals, seedling cost is too high, is difficult to use on a large scale; Adopt stock grafting to grow seedlings and to obtain a large amount of nursery stocks, but the nursery stock region adaptability that obtains because of factors, particularly grafting such as region adaptability, graft survival rate, the recruitment of stock are many is relatively poor, is often freezed to death in winter, so still can't use on a large scale; The test of the cuttage and seedling culture carried out in recent years, seedling cost is low, the seedling quality of acquisition is good, can match in excellence or beauty with the nursery stock that group training method obtains, but because of a large amount of jujube head of needs and branch as propagating materials, also be difficult to large-scale popularization; December 26 calendar year 2001, disclosed Inner Mongol comrade's Wang Fuxi of biology department of college of education patent of invention (publication number CN1327718A) took down breadboard cultivation stage with original group cultivation seedling process, saved great amount of manpower, electric power, carbohydrate and agar etc., greatly reduce contaminated may going, thereby reduced seedling cost, but in essence, this method just with plugged ear with MS medium immersion treatment after again cuttage in matrix, and the skewer fringe Cheng Miaohou of cuttage then may not obtain plugged ear more from it, want breeding in a year and still will go up a large amount of jujube head and the branches of clip several times from other trees, still there is the problem of " adopting the fringe difficulty " in this process, carry out the jujube tree seedling propagation on a large scale with this technology, still inconvenient.For many years, the farming of numerous scientific workers and jujube is wished to obtain a kind of economic and practical and can be used for extensive, as to produce the jujube tree high quality seedling in enormous quantities method.
Summary of the invention
Purpose of the present invention is exactly to overcome the defective that existing multiple jujube tree is grown seedlings; provide a kind of, thoroughly solve " bottleneck " problem of the quality and quantity of nursery stock in the jujube tree development by utilizing the jujube tree shoot to realize economic and practical, low-cost, as to be applicable to large-scale production high-quality date sapling wood jujube tree propagation technique.
The object of the present invention is achieved like this:
A kind of method for tender branch cutting propagation of Linze jujube comprises following method and step:
A, spray are selected the treelet semi-lignified of jujube tree, the spray that grows out under hot conditions; The spray acquisition time is mid-June～mid-July, and is the cuttage and seedling culture time, synchronous therewith;
B, collection spray, be advisable the morning, shears spray, avoids carrying out under the sunlit condition in the cool place; The spray of adopting is back placed in the cool, or immerses in the water, or the wet piece of sack hides; The length of cuttage spray is 15～20 centimeters, and the straight snips otch is removed the branches and leaves in 5 centimeters of the bottoms, keeps 2～3 leaves on the plugged ear; It is neat to shear good spray lower cut end pendulum, ties up and is a bundle, urge the root chemicals treatment immediately for per 30;
With concentration be the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium, the 1PPM of 6-benzyl purine, the 10PPM of indolebutyric acid, the 10PPM of 150PPM tremble zinc, 5PPM glycine, 0.1PPM biotin be mixed with mixed solution by following weight portion proportioning with aquae destillata or cold water:
Tremble 0.2～1 part on zinc of 30～150 parts of indolebutyric acids, 0.5～1.5 part of 6-benzyl purine, 1～2 part of hydrochloric acid sulphur ammonium, hydrochloric acid pyrrole, 2～4 parts of glycine, 0.01～0.02 part of biotin, 200000 parts in water;
The spray plugged ear base portion of tying up is immersed in the mixed solution for preparing, and the degree of depth is 2～4 centimetres, soaks 40 minutes;
C, with thick river sand last and contain the loam layer that becomes thoroughly decomposed in the following seedbed of construction, river sand thickness is in 7 centimeters, the back of pouring water keeps 4～5 centimeters, contains the loam layer in 30 centimeters; Plough deeply 30 centimeters after the soil layer fertilising, rake after ploughing deeply, the shop is husky after treading;
Before use in D, seedbed, to the sand bed disinfection; Little shed insulation, light modulation, damping are buckled in the seedbed, the condition that controls environment and sterilization treatment:
D.1 humidity: will remain in 20 days after the relative air humidity cuttage more than 90%, reduce gradually later on, throw off little shed to 30 days; Soil humidity keeps moistening getting final product, and avoids ponding and becomes waterlogging;
D.2 temperature: night 15 degree and more than, daytimes 28 degree;
D.3 illumination: keep 1.2 ten thousand LUX in preceding 10 days, kept 30,000 LUX on the 10th～20 day, ascending after 20 days, adapt to gradually, the shading at noon, sunlight direct projection sooner or later, complete sunlight direct projection entered the normal management stage in the booth until 30 days;
D.4 use 1000 times of liquid of thiophanate methyl to spray the seedbed once weekly;
D.5 spray leaf fertilizer after the 10th day, per 7 days are once; To spray 0.4% urea liquid, spray 0.3% potassium dihydrogen phosphate autumn; Opened to cheat in the 8th day and callus occurs, saw root on the 15th day and began growth, root system can be grown 10-20 centimetre in 50 days, beginning in the 15th～18 day, and the main bud of plugged ear upper end begins to sprout, and takes out living cane branches and leaves, forms complete plant, carries out the field production cultivation spring in next year;
E, the cuttage degree of depth are set upright cutting for 3～4 centimetres, cutting density 5 * 5cm, 5 * 8cm, 5 * 10cm, 5 * 12cm, 5 * 15cm;
Before the cuttage, watered in the seedbed, after the cuttage, to transplanting a cutting hut, cuttage is carried out simultaneously with water spray, and a slice spray a slice is inserted in insert edge spray in limit.；
F, 30～40 days cycles of taking root sterilize all the time, finish cuttage, water spray after, carry out first time sterilization, afterwards once every sterilization in 1 day, sterilization and the same day last water spray carry out synchronously;
The control and regulation of G, temperature, humidity
The spray callus forms 7～12 days, and 15～17 days begin to take root, and come to an end up to 20～25 days; To control to adjust temperature, humidity during taking root, spray at 10 o'clock in morning every day is water for the first time, sprayed the 2nd time water by 12 o'clock, each 1～2 minute of water spraying time, afternoon 1～3 o'clock, water spray shortening blanking time was sprayed once in about 20～30 minutes, and water spraying time extends to 2～3 minutes, after at 4 o'clock in afternoon, stop water spray, meet the cloudy day, do not spray water;
H, hardening
35～40 days beginning hardenings, the hardening job order is: before early 10 o'clock, after at 6 o'clock in afternoon, every day, each opened wide 1～2 hour sooner or later with little shed canopy film, ventilated, and noon, the button canopy was preserved moisture during high temperature, transition later on is to detain canopy daytime, takes off canopy night, removes little shed after the week.
Described spray comprises that jujube head, secondary branch and jujube hang.
The raw material of described mixed solution and proportioning can replace with:
0.5～1.5 part of 60～300 parts of 6-benzyl purine of methyl
Tremble 0.2～1 part on zinc of 1～2 part of hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium
0.01～0.02 part of 2～4 parts of biotin of glycine
200000 parts in water
The raw material of described mixed solution and proportioning can also replace with:
60～300 parts of 30～150 parts of methyls of indolebutyric acid
1～2 part of 0.5～1.5 part of hydrochloric acid sulphur of 6-benzyl purine ammonium
Tremble 2～4 parts of 0.2～1 part of glycine of zinc of hydrochloric acid pyrrole
200000 parts in 0.01～0.02 part of water of biotin
Described sand bed is sterilized made disinfectant with potassium permanganate, and one mu of usefulness 100 gram potassium permanganate is diluted in 800 kg of water.
Described cutting density is 5 * 12cm～5 * 15cm.
Jujube tree epicormic branch cuttage technology of the present invention is an applicable technology, and that uses jujube tree gives birth to shoot then as propagating materials, is the method for duplicating the jujube tree nursery stock.Its reproduction process comes down to a process of getting root, by this process, behind growth hormone and growth hormone synergist solution immersion root, the unrooted branch is become take root in strain.Just under artificial condition, have the characteristic of regeneration capacity according to plant, impel himself mitogenetic, the spray with plant duplicates parent, reproduces the process of whole plant.Good effect of the present invention is the defective that has overcome existing multiple jujube tree seedling-cultivating method, found a kind of economical and practical method of large-scale production jujube tree high quality seedling, compare with original state-of-the-art group of cultivation seedling-growing method, this technology, aspect two, breakthrough is arranged: 1, offspring can be taken from directly, solve jujube tree and grown seedlings and get the problem of root difficulty; 2, can bring out the truly nursery stock of pure high-quality, solve " bottleneck " problem that the difficulty of growing seedlings for a long time in the applicable technology field is given pure high-quality.
Embodiment
Embodiment 1
1, spray is selected the treelet semi-lignified, the jujube head that grows out under hot conditions survives as epicormic branch cuttage.Spray cortex children is tender, and meristematic tissue is energetic, and regeneration capacity is strong, and cuttage is easy to take root and survives, and the seedling quality that brings out is good.
The spray acquisition time is mid-June～mid-July, and is the cuttage and seedling culture time, synchronous therewith.
2, avoid the morning sunlit condition down cut to gather spray in the cool place, the spray of adopting is back placed in the cool, reduce moisture loss, spray is with gathering with processing as far as possible, and the length of cuttage spray is 15 centimeters, the straight snips otch is removed the branches and leaves in 5 centimeters of the bottoms, keeps 2～3 leaves on the plugged ear.Neat to the spray lower cut end sheared pendulum, tie up and be a bundle, urge the root chemicals treatment immediately for per 30.The plastic basin that diameter is 0.5 meter is handled spray 2500 strains (branch).
With aquae destillata or cold water compound concentration be the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 1g, 1PPM of 6-benzyl purine 0.5g, 10PPM of indolebutyric acid 30g, the 10PPM of 150PPM tremble zinc 0.2g, 5PPM the biotin 0.01g mixed solution of glycine 2g, 0.1PPM standby.The spray plugged ear root of tying up is immersed in the growth hormone and growth hormone synergist mixed solution for preparing, and the degree of depth is 4 centimetres, soaks 40 minutes.
3, with thick river sand and the seedbed that contains the loam layer construction of becoming thoroughly decomposed be desirable seedbed.River sand is last, and soil layer is following.Thick river sand short texture, ventilation and water retention property are good, can satisfy take root during ventilative, infiltration demand; Sheep cow dung is the soil layer of main fertilizer, and the required nutrition of taking root can be provided.River sand thickness is no more than 7 centimeters, and the back of pouring water keeps 4, contains the loam layer and is not less than 30 centimeters.Plough deeply 30 centimeters after the soil layer fertilising, rake after ploughing deeply, tread after raking, tread shop, back sand.
4, before use in the seedbed, be to the sand bed disinfection.Disinfectant potassium permanganate, one mu of usefulness 100 gram is diluted in 800 kg of water and uses.Little shed insulation, light modulation, damping are buckled in the seedbed, the condition that controls environment and sterilization nursing:
(1) humidity: remain on more than 90% in preceding 20 days after the relative air humidity cuttage, reduce gradually later on, threw off little shed to 30 days, soil humidity keeps moistening getting final product, and avoids ponding and becomes waterlogging.
(2) temperature: be not less than 15 degree night, spend daytimes 25.
(3) illumination: keep 1.2 ten thousand LUX in preceding 10 days after the cuttage, kept 30,000 LUX on the 10th～20 day, ascending after 20 days, adapt to gradually, suitable shading at noon, sunlight direct projection sooner or later, complete sunlight direct projection entered the normal management stage in the booth until 30 days.
(4) use 1000 times of liquid of thiophanate methyl to spray the seedbed once weekly.
Spray leaf fertilizer after (5) the 10th days, per 7 days are once; Spray 0.4% urea liquid and squirt with the blade face and be as the criterion, spray 0.3% potassium dihydrogen phosphate autumn and squirt with the blade face and be as the criterion; Opened to cheat in the 8th day and callus occurs, saw root on the 15th day and began growth, root system can be grown to 10～20 centimetres in 50 days, beginning in the 15th day, and the main bud of plugged ear upper end begins to sprout, and takes out living cane branches and leaves, forms complete plant, carries out the field production cultivation spring in next year.
5,4 centimetres of the cuttage degree of depth are set upright cutting, cutting density 5 * 12cm.
Before the cuttage, watered in the seedbed, after the cuttage, to transplanting a cutting hut, cuttage is carried out simultaneously with water spray, and a slice spray a slice is inserted in insert edge spray in limit.To the adjusting of temperature, humidity, begin and will catch up with this moment.
6,30 days cycles of taking root are run through sterilization all the time, and sterilization is regularly carried out, finish cuttage, water spray after, carry out sterilization first time, sterilized once every 1 day afterwards.The sterilization with the same day last water spray carry out synchronously.
7, the control and regulation of temperature, humidity
The spray callus forms 7 days, and 15 days begin to take root, and come to an end up to 20 days; To control to adjust temperature, humidity during taking root, spray at 10 o'clock in morning every day is water for the first time, sprayed the 2nd time water by 12 o'clock, each 1 minute of water spraying time, afternoon 1～3 o'clock, water spray shortening blanking time was sprayed once in 20 minutes, and water spraying time extends to 2 minutes, after at 4 o'clock in afternoon, stop water spray, meet the cloudy day, do not spray water.
9, hardening
35 days beginning hardenings, the hardening job order is: before early 10 o'clock, after at 6 o'clock in afternoon, every day, each opened wide 1 hour sooner or later with little shed canopy film, ventilated, and noon, the button canopy was preserved moisture during high temperature, progressively increase ventilation time later on, transition is to detain canopy daytime, takes off canopy night, removes little shed after the week and gets final product.
Embodiment 2
1, spray is selected the treelet semi-lignified, the secondary branch that grows out under hot conditions survives as epicormic branch cuttage.Spray cortex children is tender, and meristematic tissue is energetic, and regeneration capacity is strong, and cuttage is easy to take root and survives, and the seedling quality that brings out is good.
The spray acquisition time is mid-June～mid-July, and is the cuttage and seedling culture time, synchronous therewith.
2, avoid the morning sunlit condition down cut to gather spray in the cool place, the spray of adopting is back placed in the cool, reduce moisture loss, spray is with gathering with processing as far as possible, and the length of cuttage spray is 20 centimeters, the straight snips otch is removed the branches and leaves in 5 centimeters of the bottoms, keeps 2～3 leaves on the plugged ear.Neat to the spray lower cut end sheared pendulum, tie up and be a bundle, urge the root chemicals treatment immediately for per 30.
With aquae destillata or cold water compound concentration be the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 2g, 1PPM of 6-benzyl purine 1.5g, 10PPM of indolebutyric acid 150g, the 10PPM of 150PPM tremble zinc 1g, 5PPM the biotin 0.02g mixed solution of glycine 4g, 0.1PPM standby.The spray plugged ear root of tying up is immersed in the growth hormone and growth hormone synergist mixed solution for preparing, and the degree of depth is 2 centimetres, soaks 40 minutes.
3, with thick river sand and the seedbed that contains the loam layer construction of becoming thoroughly decomposed be desirable seedbed.River sand is last, and soil layer is following.Thick river sand short texture, ventilation and water retention property are good, can satisfy take root during ventilative, infiltration demand; Sheep cow dung is the soil layer of main fertilizer, and the required nutrition of taking root can be provided.7 centimeters of river sand thickness, the back of pouring water keeps 5 centimeters, contains the loam layer and is not less than 30 centimeters.Plough deeply 30 centimeters after the soil layer fertilising, rake after ploughing deeply, tread after raking, tread shop, back sand.
4, before use in the seedbed, be to the sand bed disinfection.Disinfectant potassium permanganate, one mu of usefulness 100 gram is diluted in 800 kg of water and uses.Little shed insulation, light modulation, damping are buckled in the seedbed, the condition that controls environment and sterilization nursing:
(1) humidity: remain on more than 90% in preceding 20 days after the relative air humidity cuttage, reduce gradually later on, threw off little shed to 30 days, soil humidity keeps moistening getting final product, and avoids ponding and becomes waterlogging.
(2) temperature: be not less than 15 degree night, spend daytimes 28.
(3) illumination: keep 1.2 ten thousand LUX in preceding 10 days after the cuttage, kept 30,000 LUX on the 10th～20 day, ascending after 20 days, adapt to gradually, suitable shading at noon, sunlight direct projection sooner or later, complete sunlight direct projection entered the normal management stage in the booth until 30 days.
(4) use 1000 times of liquid of thiophanate methyl to spray the seedbed once weekly.
Spray leaf fertilizer after (5) the 10th days, per 7 days are once; Spray 0.4% urea liquid and squirt with the blade face and be as the criterion, spray 0.3% potassium dihydrogen phosphate autumn and squirt with the blade face and be as the criterion; Opened to cheat in the 8th day and callus occurs, saw root on the 15th day and began growth, root system can be grown to 10～20 centimetres in 50 days, beginning in the 18th day, and the main bud of plugged ear upper end begins to sprout, and takes out living cane branches and leaves, forms complete plant, carries out the field production cultivation spring in next year.
5,3 centimetres of the cuttage degree of depth are set upright cutting, cutting density 5 * 15cm.
Before the cuttage, watered in the seedbed, after the cuttage, to transplanting a cutting hut, cuttage is carried out simultaneously with water spray, and a slice spray a slice is inserted in insert edge spray in limit.To the adjusting of temperature, humidity, begin and will catch up with this moment.
6,40 days cycles of taking root are run through sterilization all the time, and sterilization is regularly carried out, finish cuttage, water spray after, carry out sterilization first time, sterilized once every 1 day afterwards.The sterilization with the same day last water spray carry out synchronously.
7, the control and regulation of temperature, humidity
The spray callus forms 7 days, and 17 days begin to take root, and come to an end up to 25 days; To control to adjust temperature, humidity during taking root, spray at 10 o'clock in morning every day is water for the first time, sprayed the 2nd time water by 12 o'clock, each 2 minutes of water spraying time, afternoon 1～3 o'clock, water spray shortening blanking time was sprayed once in 30 minutes, and water spraying time extends to 3 minutes, after at 4 o'clock in afternoon, stop water spray, meet the cloudy day, do not spray water.
9, hardening
40 days beginning hardenings, the hardening job order is: before early 10 o'clock, after at 6 o'clock in afternoon, every day, each opened wide 1 hour sooner or later with little shed canopy film, ventilated, and noon, the button canopy was preserved moisture during high temperature, progressively increase ventilation time later on, transition is to detain canopy daytime, takes off canopy night, removes little shed after the week and gets final product.
Embodiment 3
1, spray is selected the treelet semi-lignified, the jujube that grows out under hot conditions hangs as epicormic branch cuttage and survive.Spray cortex children is tender, and meristematic tissue is energetic, and regeneration capacity is strong, and cuttage is easy to take root and survives, and the seedling quality that brings out is good.
The spray acquisition time is mid-June～mid-July, and is the cuttage and seedling culture time, synchronous therewith.
2, getting the 6-benzyl purine 1.5g of methyl 60g, 20PPM, is that tremble zinc 0.2g, glycine 2g, 0.05PPM biotin 0.01g mixed solution of the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 1g, the 1PPM of 5PPM is standby with aquae destillata or cold water 200000g compound concentration.The spray plugged ear root of tying up is immersed in the mixed solution for preparing, and the degree of depth is 4 centimetres, soaks 80 minutes.
3, with embodiment 1;
4, with embodiment 1;
5,3 centimetres of the cuttage degree of depth are set upright cutting, cutting density 5 * 10cm.
6, with embodiment 1;
7, with embodiment 1;
8, with embodiment 1;
9, with embodiment 1;
Embodiment 4
1, with embodiment 2;
2, get the 6-benzyl purine 0.5g of methyl 300g, 20PPM, tremble zinc 1g, glycine 4g, 0.05PPM biotin 0.02g of the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 2g, the 1PPM of 5PPM is standby with cold water 200000g preparation mixed solution.
The spray plugged ear root of tying up is immersed in the mixed solution for preparing, and the degree of depth is 4 centimetres, soaks 60 minutes
3, with embodiment 2;
4, with embodiment 1
5,4 centimetres of the cuttage degree of depth are set upright cutting, cutting density 5 * 8cm.
6, with embodiment 2;
7, with embodiment 2;
8, with embodiment 2;
9, with embodiment 2;
Embodiment 5
1, with embodiment 1;
2, get the 6-benzyl purine 1.5g of indolebutyric acid 150g, methyl 300g, 20PPM, tremble zinc 1g, glycine 4g, 0.05PPM biotin 0.02g of the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 2g, the 1PPM of 5PPM is standby with pure water 200000g preparation mixed solution.
The spray plugged ear root of tying up is immersed in the mixed solution for preparing, and the degree of depth is 4 centimetres, soaks 60 minutes.
3, with embodiment 1;
4, with embodiment 1;
5,3 centimetres of the cuttage degree of depth are set upright cutting, cutting density 5 * 5cm.
6, with embodiment 1;
7, with embodiment 1;
8, with embodiment 1;
9, with embodiment 1;
Embodiment 6
1, with embodiment 1;
2, get the 6-benzyl purine 0.5g of indolebutyric acid 30g, methyl 60g, 20PPM, tremble zinc 0.2g, glycine 2g, 0.05PPM biotin 0.01g of the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium 1g, the 1PPM of 5PPM is standby with aquae destillata 200000g preparation mixed solution.
The spray plugged ear root of tying up is immersed in the mixed solution for preparing, and the degree of depth is 4 centimetres, soaks 40 minutes.
3, with embodiment 1;
4, with embodiment 1;
5, with embodiment 1;
6, with embodiment 1;
7, with embodiment 1;
8, with embodiment 1;
9, with embodiment 1;
Claims (7)
1. method for tender branch cutting propagation of Linze jujube is characterized in that comprising following method and step:
A, spray are selected the treelet semi-lignified of jujube tree, the spray that grows out under hot conditions; The spray acquisition time is mid-June～mid-July, and is the cuttage and seedling culture time, synchronous therewith;
B, collection spray, be advisable the morning, shears spray, avoids carrying out under the sunlit condition in the cool place; The spray of adopting is back placed in the cool, or immerses in the water, or the wet piece of sack hides; The length of cuttage spray is 15～20 centimeters, and the straight snips otch is removed the branches and leaves in 5 centimeters of the bottoms, keeps 2～3 leaves on the plugged ear; It is neat to shear good spray lower cut end pendulum, ties up and is a bundle, urge the root chemicals treatment immediately for per 30;
With concentration is that tremble glycine, the 0.1PPM biotin of zinc, 5PPM of the hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium, the 1PPM of 6-benzyl purine, the 10PPM of indolebutyric acid, the 10PPM of 150PPM is mixed with mixed solution by following weight portion proportioning with aquae destillata or cold water:
Tremble 0.2～1 part on zinc of 30～150 parts of indolebutyric acids, 0.5～1.5 part of 6-benzyl purine, 1～2 part of hydrochloric acid sulphur ammonium, hydrochloric acid pyrrole, 2～4 parts of glycine, 0.01～0.02 part of biotin, 200000 parts in water;
The spray plugged ear base portion of tying up is immersed in the mixed solution for preparing, and the degree of depth is 2～4 centimetres, soaks 40～80 minutes;
C, with thick river sand last and contain the loam layer that becomes thoroughly decomposed in the following seedbed of construction, river sand thickness is in 7 centimeters, the back of pouring water keeps 4～5 centimeters, contains the loam layer in 30 centimeters; Plough deeply 30 centimeters after the soil layer fertilising, rake after ploughing deeply, the shop is husky after treading;
Before use in D, seedbed, to the sand bed disinfection; Little shed insulation, light modulation, damping are buckled in the seedbed, the condition that controls environment and sterilization treatment:
D.1 humidity: remain on more than 90% in preceding 20 days after the relative air humidity cuttage, reduce gradually later on, threw off little shed to 30 days; It is moistening that soil humidity keeps, and avoids ponding and become waterlogging;
D.2 temperature: night 15 degree and more than, daytimes 25～28 degree;
D.3 illumination: keep 1.2 ten thousand LUX in preceding 10 days after the cuttage, kept 30,000 LUX on the 10th～20 day, ascending after 20 days, the shading at noon, sunlight direct projection sooner or later, complete sunlight direct projection entered the normal management stage in the booth until 30 days;
D.4 use 1000 times of liquid of thiophanate methyl to spray the seedbed once weekly;
D.5 spray leaf fertilizer after the 10th day, per 7 days are once; Spray 0.4% urea liquid, spray 0.3% potassium dihydrogen phosphate autumn; Opened to cheat in the 8th day and callus occurs, saw root on the 15th day and began growth, root system can be grown 10-20 centimetre in 50 days, beginning in the 15th～18 day, and the main bud of plugged ear upper end begins to sprout, and takes out living cane branches and leaves, forms complete plant, carries out the field production cultivation spring in next year;
E, the cuttage degree of depth are set upright cutting for 3～4 centimetres, cutting density 5 * 5cm, 5 * 8cm, 5 * 10cm, 5 * 12cm, 5 * 15cm;
Before the cuttage, watered in the seedbed, after the cuttage, to transplanting a cutting hut, cuttage is carried out simultaneously with water spray, and a slice spray a slice is inserted in insert edge spray in limit;
F, 30～40 days cycles of taking root sterilize all the time, finish cuttage, water spray after, carry out first time sterilization, afterwards once every sterilization in 1 day, sterilization and the same day last water spray carry out synchronously;
The control and regulation of G, temperature, humidity
The spray callus forms 7～12 days, and 15～17 days begin to take root, and come to an end up to 20～25 days; To control to adjust temperature, humidity during taking root, spray at 10 o'clock in morning every day is water for the first time, sprayed the 2nd time water by 12 o'clock, each 1～2 minute of water spraying time, afternoon 1～3 o'clock, water spray shortening blanking time was sprayed once in 20～30 minutes, and water spraying time extends to 2～3 minutes, after at 4 o'clock in afternoon, stop water spray, meet the cloudy day, do not spray water;
H, hardening
35～40 days beginning hardenings, the hardening job order is: before early 10 o'clock, after at 6 o'clock in afternoon, every day, each opened wide 1～2 hour sooner or later with little shed canopy film, ventilated, and noon, the button canopy was preserved moisture during high temperature, transition later on is to detain canopy daytime, takes off canopy night, removes little shed after the week.
2. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that described spray comprises that jujube head, secondary branch and jujube hang.
3. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that the raw material of described mixed solution and proportioning are:
0.5～1.5 part of 60～300 parts of 6-benzyl purine of methyl
Tremble 0.2～1 part on zinc of 1～2 part of hydrochloric acid pyrrole of hydrochloric acid sulphur ammonium
0.01～0.02 part of 2～4 parts of biotin of glycine
200000 parts in water
Wherein the concentration of methyl is 20PPM.
4. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that the raw material of described mixed solution and proportioning are:
60～300 parts of 30～150 parts of methyls of indolebutyric acid
1～2 part of 0.5～1.5 part of hydrochloric acid sulphur of 6-benzyl purine ammonium
Tremble 2～4 parts of 0.2～1 part of glycine of zinc of hydrochloric acid pyrrole
200000 parts in 0.01～0.02 part of water of biotin.
5. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that described sand bed sterilization being made disinfectant with potassium permanganate, and one mu of usefulness 100 gram potassium permanganate is diluted in 800 kg of water.
6. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that described cutting density is 5 * 12cm.
7. method for tender branch cutting propagation of Linze jujube according to claim 1 is characterized in that described cutting density is 5 * 15cm.
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