CN104798487B - A kind of method promoting seed germination of Isatis Indigotica Fort - Google Patents
A kind of method promoting seed germination of Isatis Indigotica Fort Download PDFInfo
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- CN104798487B CN104798487B CN201510183781.7A CN201510183781A CN104798487B CN 104798487 B CN104798487 B CN 104798487B CN 201510183781 A CN201510183781 A CN 201510183781A CN 104798487 B CN104798487 B CN 104798487B
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- 230000001737 promoting effect Effects 0.000 title claims abstract description 8
- 239000007788 liquid Substances 0.000 claims abstract description 46
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 40
- 238000000197 pyrolysis Methods 0.000 claims abstract description 40
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- 238000002360 preparation method Methods 0.000 claims abstract description 7
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- 240000008866 Ziziphus nummularia Species 0.000 claims 2
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- 235000006228 Cercis occidentalis Nutrition 0.000 description 2
- 229930191978 Gibberellin Natural products 0.000 description 2
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- 244000020191 Salix babylonica Species 0.000 description 2
- 235000002493 Salix babylonica Nutrition 0.000 description 2
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Abstract
The invention discloses a kind of method promoting seed germination of Isatis Indigotica Fort, step is：Seed sprout before, with plant growth regulator, Radix Isatidis seed is carried out with pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, jujube nucleus and walnut shell carry out the dry distillation liquid that dry distilling obtains.The present invention carries out pretreatment as plant growth regulator to Radix Isatidis seed using plant dry distillation liquid, and preprocess method is simply it is easy to operation, pollution-free, can significantly improve the germination rate of Radix Isatidis seed, high financial profit.And plant dry distillation liquid used be Semen Crataegi, walnut shell, the core of these three plants of jujube nucleus, shell carry out the dry distillation liquid that dry distilling obtains, its preparation method is simple to operate, succinctly quick, it is easily achieved, raw materials used for plant refuse it is achieved that making full use of of waste, turn waste into wealth, low cost, is easy to industrialized production.
Description
Technical field
The present invention relates to a kind of method promoting seed germination of Isatis Indigotica Fort is and in particular to one kind extracts using from plant
The dry distillation liquid coming, to promote the method in seed germination of Isatis Indigotica Fort, belongs to seed processing technology field.
Background technology
With being filled with of " back to nature " trend, traditional natural plant is increasingly favored by people.Radix Isatidis come
Come from the dry root of cruciferae isatis Isatis indigotica Fort., there is the work(such as heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving
Effect, is that China commonly uses one of large Chinese crude drug.Domestic and international market increasingly increases to the demand of chromatogram of Radix Isatidis at present, produces rule
Mould and cultivated area constantly expand.In production, Radix Isatidis mainly adopt seed growing, and seed is sprouted and is directly connected to the medical material later stage
Growth.For a long time, the research with regard to improving seed germination rate becomes the focus of Radix Isatidis research.Sprout for improving seed
Rate, how production carries out seed using growth regulators such as gibberellins and sprouts pre-treatment, gibberellins are synthetic, there is cost
High, seriously polluted the problems such as, therefore find the approach effectively facilitating seed germination of Isatis Indigotica Fort for the product improving chromatogram of Radix Isatidis
Amount is more and more important.
Dry distillation liquid refers to be put into vegetable material the flue gas natural cooling liquefaction emerge in heating carbonization process in retort
The concentrated liquid obtaining.In recent years, the medical and edibility of dry distillation liquid is taken seriously, and is widely used in sterilization, disease prevention, food
The fields such as product are anti-corrosion, seasoning, fruit freshness preserving.It is rarely reported however, dry distillation liquid being used for Chinese crude drug seed and sprouting.
Content of the invention
It is an object of the invention to provide a kind of promote seed germination of Isatis Indigotica Fort method, the method using plant dry distillation liquid as
Plant growth regulator carries out pretreatment to Radix Isatidis seed, can significantly improve seed germination of Isatis Indigotica Fort, improves plate on producing
Blue Root yield is significant.
At present, dry distillation liquid is used in medicine mainly as sterilization, antipathogenic composition, has no in promoting Chinese crude drug seed to sprout
Report.Inventor finds under study for action, and dry distillation liquid can be remarkably promoted seed germination of Isatis Indigotica Fort as plant growth regulator, and
And from the experimental results, the dry distillation liquid of different plant origins is different to the facilitation of seed germination of Isatis Indigotica Fort.Inventor
Through a large amount of screening experiment, finally give the dry distillation liquid that can effectively facilitate seed germination of Isatis Indigotica Fort, and specifically have studied this and done
The preparation method of distillate and using method, thus be to be used for promoting seed germination of Isatis Indigotica Fort to provide important technology by dry distillation liquid
Support.
Concrete technical scheme of the present invention is as follows：
A kind of method promoting seed germination of Isatis Indigotica Fort, is characterized in that：Before seed is sprouted, with plant growth regulator pair
Radix Isatidis seed carries out pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, acid
Semen Jujubae and walnut shell carry out the dry distillation liquid that dry distilling obtains, and this dry distillation liquid is referred to as plant dry distillation liquid.
Plant growth regulator used by the present invention is a kind of plant dry distillation liquid, and it is preferably mass ratio is 11:2:2 or 13:
1:1 Semen Crataegi, jujube nucleus and walnut shell carry out the dry distillation liquid that dry distilling obtains.
Plant growth regulator used by the present invention, prepares in accordance with the following methods：By Semen Crataegi, jujube nucleus and walnut shell
By proportioning mixing, carry out dry distilling at 140～145 DEG C, 240～245 DEG C, 300～360 DEG C respectively, at a temperature of merging this three sections
The dry distillation liquid of gained, the dry distillation liquid remove impurity after merging obtains plant growth regulator.
When preparing plant growth regulator, it is incubated 1-2h at 140～145 DEG C, be incubated 2-3 hour at 240～245 DEG C,
300～360 DEG C of insulation 1-2 hours.
When preparing plant growth regulator, the dry distillation liquid after merging adopts following method remove impurity：By the dry distillation liquid after merging
Stir, stratification, take supernatant；Supernatant is distilled, gained distillate is filtered with after activated carbon process, gained filtrate
It is plant dry distillation liquid.
During dry distillation liquid remove impurity, supernatant is in 100～105 DEG C of air-distillations.
During dry distillation liquid remove impurity, supernatant distillation time is 2h.
During dry distillation liquid remove impurity, distillate adds the activated carbon of its quality 10%.
During dry distillation liquid remove impurity, activated carbon is processed 45 minutes at 95 DEG C, carries out remove impurity.
The present invention promotes, in the method for seed germination of Isatis Indigotica Fort, with plant growth regulator, seed to be carried out with the mistake of pretreatment
Cheng Wei：By plant growth regulator and water according to 1:The volume ratio mixing of 500-2000, obtains preprocessing solution, and pretreatment is molten
Liquid is sprayed at Radix Isatidis the surface of the seed, carries out pretreatment to Radix Isatidis seed.
The present invention promotes in the method for seed germination of Isatis Indigotica Fort, and plant growth regulator is preferably 1 with the volume ratio of water:
1000.
The present invention promotes in the method for seed germination of Isatis Indigotica Fort, and during Radix Isatidis Seeds preprocess, the every 4h of preprocessing solution sprays
Spill once, continuously spray 48h.
The present invention promote seed germination of Isatis Indigotica Fort method in, during Radix Isatidis Seeds preprocess, spray preprocessing solution with
Drench kind of a skin to be advisable.
The present invention promotes in the method for seed germination of Isatis Indigotica Fort, selects the Radix Isatidis seed of full seed health to be sprouted
Send out.
The present invention promotes, in the method for seed germination of Isatis Indigotica Fort, first Radix Isatidis seed to be disappeared with liquor natrii hypochloritises
Poison, is then rinsed well with water, then carries out pretreatment with plant growth regulator to Radix Isatidis seed.Sodium hypochlorite used is molten
Liquid concentration can be 0.525g L-1, sterilization 10min about.
According to the method described above seed is carried out after pretreatment, seed is placed in the damp sand of water content 60% and sprouts, temperature：
25 DEG C/20 DEG C (day night)；Illumination：14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m2·s)；Relative humidity：65-70%.
After tested, the Radix Isatidis seed through pretreatment significantly improves than the not germination rate through pretreatment.
The present invention carries out pretreatment, pretreatment side as plant growth regulator to Radix Isatidis seed using plant dry distillation liquid
Method is simply it is easy to operation, pollution-free, can significantly improve the germination rate of Radix Isatidis seed, high financial profit.And plant used does
Distillate is Semen Crataegi, walnut shell, the core of these three plants of jujube nucleus, shell carry out the dry distillation liquid that dry distilling obtains, and its preparation method is grasped
Make simple, succinctly quickly, it is easy to accomplish, raw materials used for plant refuse it is achieved that making full use of of waste, turn waste into wealth,
Low cost, is easy to industrialized production.
Specific embodiment
The present invention will be further elaborated below by specific embodiment it should be appreciated that, the description below is only
In order to explain the present invention, its content is not limited.
The preparation of plant growth regulator
Embodiment 1
Take Semen Crataegi, jujube nucleus and walnut shell 1.5kg respectively, with mass ratio for 1:1:1 addition retort, is heated to 140
～145 DEG C, it is incubated 1 hour, continues to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and maintains 1
Hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will
Supernatant adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirs
Mix and be heated to 95 DEG C, maintain 45 minutes, filter, collect filtrate.Filtrate be the present invention plant growth regulator it is also possible to
Referred to as plant dry distillation liquid, similarly hereinafter.
Embodiment 2
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 6:2:3 addition retorts, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Embodiment 3
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 11:2:2 addition retorts, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Embodiment 4
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 13:1:1 addition retort, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Comparative example 1
Method according to embodiment 3 prepares plant growth regulator, and except for the difference that, plant source used is：Quercus acutissima Carr.
(Magnolia denudata), Chinese scholar tree (Sophora japonica), yellow rose (Rosa xanthina Lindl) these three
The trunk of plant, their mass ratio is 1:1:1.
Comparative example 2
Method according to embodiment 3 prepares plant growth regulator, and except for the difference that, plant source used is：Cercis
(Cercis chinensin Bunge), Salix babylonica L. (Salix babylonica), these three plants of chinaberry (Melia azedarach)
The trunk of thing, their mass ratio is 1:1:1.
The application examples of plant growth regulator treatment Radix Isatidis seed
1st, choose the Radix Isatidis seed of full seed health, use 0.525g L-1Hypochlorite disinfectant 10min, then with steaming
Distilled water is rinsed 3 times.
2nd, prepare preprocessing solution：By above-described embodiment and comparative example preparation plant growth regulator individually with water
According to 1:500、1:1000 and 1:2000 volume ratio mixing, is made into different preprocessing solutions.
3rd, the pretreatment of Radix Isatidis seed：The preprocessing solution of preparation is sprayed on respectively the Radix Isatidis kind sublist disinfecting
Face, is advisable with drenching kind of a skin, and every 4h sprays once, uninterruptedly sprays, carry out the blank sprayed using pure water simultaneously in 48h
Comparison, each processes 6 repetitions.
4th, seed is sprouted：Pretreated Radix Isatidis seed is placed in the damp sand of water content 60% and sprouts, temperature：25
DEG C/20 DEG C (day night)；Illumination：14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m2·s)；Relative humidity：65-70%.Often
Its record seed is sprouted in quantity (dash forward to break in the seed coat by radicle and be defined) germination process and is kept the skin wet in time to guarantee seed sprouting institute
The moisture needing.25d terminates to sprout experiment, measures all seedling root length alive, fresh weight, counts germination rate, measures amylase activity
(enzymatic activity is higher, and germination rate is higher).The mensure of amylase activity：Amylase activity adopts ultraviolet method, big with OD value per minute
Little change represents diastatic relative activity.
After each group Radix Isatidis seed is cultivated 25 days according to the method described above, each index is as follows：
Table 1 each group seed germination of Isatis Indigotica Fort rate and growth of seedling situation
Can be seen that plant growth regulator and the blank of the embodiment of the present invention 1,2,3,4 from the data of above-mentioned table 1
Compare the germination rate of raising Radix Isatidis seed that all can be different degrees of, seed starches enzymatic activity, fresh weight and radicle length, and pass through
Contrast between them can be seen that embodiment 3 and the facilitation of embodiment 4 is more significant, and embodiment 1,2 is taken second place.By this
Invention plant growth regulator can be seen that the plant growing tune used by the present invention with the contrast of comparative example plant growth regulator
Section agent can significantly improve the germination rate of Radix Isatidis seed, seed starches enzymatic activity, fresh weight and radicle length.
Claims (8)
1. a kind of method promoting seed germination of Isatis Indigotica Fort, is characterized in that：Before seed is sprouted, with plant growth regulator to plate
Blue root seed carries out pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, Ziziphi Spinosae
Core and walnut shell carry out the dry distillation liquid that dry distilling obtains, and the preparation method of plant growth regulator is：By Semen Crataegi, jujube nucleus and core
Peach shell is pressed proportioning and is mixed, and carries out dry distilling respectively, merge this three sections of temperature at 140～145 DEG C, 240～245 DEG C, 300～360 DEG C
The dry distillation liquid of the lower gained of degree, the dry distillation liquid remove impurity after merging obtains plant growth regulator；With plant growth regulator to seed
The process carrying out pretreatment is：By plant growth regulator and water according to 1:The volume ratio mixing of 500-2000, obtains pretreatment
Solution, preprocessing solution is sprayed at Radix Isatidis the surface of the seed, carries out pretreatment to Radix Isatidis seed.
2. method according to claim 1, is characterized in that：The mass ratio 11 of Semen Crataegi, jujube nucleus and walnut shell:2:2 or
13:1:1.
3. method according to claim 1, is characterized in that：During Radix Isatidis Seeds preprocess, the every 4h of preprocessing solution sprays
Once, continuously spray 48h.
4. method according to claim 1, is characterized in that：During Radix Isatidis Seeds preprocess, spray preprocessing solution to drench
Wet kind of skin is advisable.
5. the method according to claim 1,2 or 3, is characterized in that：With plant growth regulator, Radix Isatidis seed is carried out
Before pretreatment, first Radix Isatidis seed is carried out disinfection with liquor natrii hypochloritises, then rinsed well with water.
6. method according to claim 1, is characterized in that：When preparing plant growth regulator, it is incubated at 140～145 DEG C
1-2h, is incubated 2-3 hour at 240～245 DEG C, is incubated 1-2 hour at 300～360 DEG C.
7. method according to claim 1, is characterized in that：When preparing plant growth regulator, the dry distillation liquid after merging is adopted
With following method remove impurity：By merge after dry distillation liquid stir, stratification, take supernatant；Supernatant is distilled, gained steams
Distillate is filtered with after activated carbon process, and gained filtrate is plant growth regulator.
8. method according to claim 7, is characterized in that：When preparing plant growth regulator, supernatant is 100～105
DEG C air-distillation 2h；Distillate adds the activated carbon of its quality 10% to process 45 minutes at 95 DEG C.
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| BR112020004585B1 (en) * | 2017-10-17 | 2024-01-16 | Kao Corporation | METHOD FOR IMPROVING THE WATER RESISTANCE OF A GRANULATED SOIL MATERIAL |
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| CN101273730A (en) * | 2008-05-16 | 2008-10-01 | 南京林业大学 | Ginko pyroligneous and its applications in promoting germination of crop seeds and growth of seedlings |
| CN101502315A (en) * | 2007-05-31 | 2009-08-12 | 北京凯瑞创新医药科技有限公司 | Method for preparing dry distillation liquid for jujube core |
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| JP4702677B2 (en) * | 2003-12-19 | 2011-06-15 | 大幸Ｔｅｃ株式会社 | Seed disinfectant |
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