CN106612704A - Pre-treatment method of isatis root culture - Google Patents

Pre-treatment method of isatis root culture Download PDF

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Publication number
CN106612704A
CN106612704A CN201510736423.4A CN201510736423A CN106612704A CN 106612704 A CN106612704 A CN 106612704A CN 201510736423 A CN201510736423 A CN 201510736423A CN 106612704 A CN106612704 A CN 106612704A
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CN
China
Prior art keywords
isatis root
temperature
treatment
isatis
room
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Pending
Application number
CN201510736423.4A
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Chinese (zh)
Inventor
马占江
吕国军
李灵吉
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Anguo Juyaotang Pharmaceutical Co Ltd
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Anguo Juyaotang Pharmaceutical Co Ltd
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Priority to CN201510736423.4A priority Critical patent/CN106612704A/en
Publication of CN106612704A publication Critical patent/CN106612704A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a pre-treatment method of isatis root culture, and particularly relates to the technical field of planting. The method comprises the following steps of 1, screening: picking complete and full isatis root seeds; 2, acidification treatment: soaking the isatis root seeds screened in the step 1 by hydrochloric acid or acetic acid with the mass percentage of 0.02 to 0.05 percent for 8 to 10 hours; 3, freezing treatment: putting the isatis root seeds subjected to acidification treatment in the step 2 into a freezing room; regulating the temperature so that the temperature is lowered to -20 DEG C from the room temperature at the speed of lowering 2 DEG C every day; then, performing freezing for 10 days; next, regulating the temperature so that the temperature is raised to the room temperature from -20 DEG C at the speed of raising 2 DEG C every day; 4, water draining treatment: putting the isatis root seeds treated by the step 3 into an air-permeable room to be dried in the air until the water content is 60 percent to 80 percent and obtaining the pre-treated isatis root seeds. The pre-treatment method has the advantages that the isatis root culture pretreatment is performed; the growth period of the isatis root is shortened; the survival rate of the isatis root seeds is improved; the production quantity is improved.

Description

The preprocess method that isatis root is cultivated
Technical field
The present invention relates to planting technology field, more particularly to the preprocess method that isatis root is cultivated.
Background technology
Radix Isatidis also known as tea indigo plant, woaded blue etc..Stem is upright, green, top multi-branched, plant Glabrous, band white powder frost.There is cultivation all parts of the country.There are clearing heat and detoxicating, blood cooling and ecchymoses removing, relieving sore throat and acesodyne.Leaf also can extract blue dyes;Seed extracts oil, for industrial.This plant is biennial herb, high 40-100 centimetre;Stem is upright, green, top multi-branched, plant Glabrous, band white powder frost.
Because for biennial herb, so constraining isatis root cultivation, prior art not yet has the method for shortening isatis root growth cycle, therefore this problems demand is solved.
The content of the invention
The technical problem to be solved is to provide shortening growth cycle, improves the preprocess method that the isatis root of yield is cultivated
To solve above-mentioned technical problem, the technical solution used in the present invention is:1st, the preprocess method that a kind of isatis root is cultivated, it is characterised in that comprise the steps:
1) screen:Select complete full isatis root seed;
2) acidification:By step 1) screening isatis root seed mass fraction be 0.02%~0.05% hydrochloric acid or acetic acid soak 8~10 hours;
3) freezing processing:Treat step 2) the isatis root seed of acidification is placed in refrigerating chamber, adjusts temperature and is adjusted to -20 DEG C from the speed that room temperature starts to lower 2 DEG C daily, freezes afterwards 10 days, and then adjust temperature and be adjusted to room temperature from -20 DEG C of speed started to raise 2 DEG C daily;
4) drought stress:By step 3) the isatis root seed that processes is placed on the room-dry of air permeable to water content between 60%~80%, obtains final product the isatis root seed of pretreatment.
It is using the beneficial effect produced by above-mentioned technical proposal:The invention reside in the pretreatment cultivated isatis root, shortens its growth cycle, its survival rate is improved, improve its output.
Specific embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment a part of embodiment only of the invention, rather than the embodiment of whole.Based on the embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made belongs to the scope of protection of the invention.
Elaborate many details in order to fully understand the present invention in the following description, but the present invention can also be different from alternate manner described here to implement using other, those skilled in the art can do similar popularization in the case of without prejudice to intension of the present invention, therefore the present invention is not limited by following public specific embodiment.
The present invention comprises the steps:
1) screen:Select complete full isatis root seed;
2) acidification:By step 1) screening isatis root seed mass fraction be 0.02%~0.05% hydrochloric acid or acetic acid soak 8~10 hours;
3) freezing processing:Treat step 2) the isatis root seed of acidification is placed in refrigerating chamber, adjusts temperature and is adjusted to -20 DEG C from the speed that room temperature starts to lower 2 DEG C daily, freezes afterwards 10 days, and then adjust temperature and be adjusted to room temperature from -20 DEG C of speed started to raise 2 DEG C daily;
4) drought stress:By step 3) the isatis root seed that processes is placed on the room-dry of air permeable to water content between 60%~80%, obtains final product the isatis root seed of pretreatment.
Embodiment one
1) complete full isatis root seed is selected;
2) sowed around grain rains;
3) gather in before and after Grain in Ear next year.
In Anguo City synandrium hall pharmaceutcal corporation, Ltd cultivation base, 3 mu of planting bases are hewed out as experimental field one, plant Radix Isatidis seed.
Embodiment two
1) screen:Select complete full isatis root seed;
2) acidification:By step 1) screening isatis root seed mass fraction be 0.02%~0.05% hydrochloric acid or acetic acid soak 8~10 hours;
3) freezing processing:Treat step 2) the isatis root seed of acidification is placed in refrigerating chamber, adjusts temperature and is adjusted to -20 DEG C from the speed that room temperature starts to lower 2 DEG C daily, freezes afterwards 10 days, and then adjust temperature and be adjusted to room temperature from -20 DEG C of speed started to raise 2 DEG C daily;
4) drought stress:By step 3) the isatis root seed that processes is placed on the room-dry of air permeable to water content between 60%~80%, obtains final product the isatis root seed of pretreatment.
5) sowed around grain rains;In ought gather in before and after Grain in Ear every year.
In Anguo City synandrium hall pharmaceutcal corporation, Ltd cultivation base, 3 mu of planting bases are hewed out for experimental field two, plantation Radix Isatidis seed.
Embodiment one, two results are contrasted, on growth cycle, embodiment two is sowed then around grain rains, is gathered in around Grain in Ear, and growth cycle is 3 months, substantially shorten, in survival rate, embodiment two is 99.95%, and embodiment one is 85%, on isatis root seed output, embodiment two receives twenty percent than embodiment more than.
From the foregoing, it will be observed that the invention reside in isatis root cultivate pretreatment, its growth cycle can be shortened, improve its survival rate, improve its output.

Claims (1)

1. the preprocess method that a kind of isatis root is cultivated, it is characterised in that comprise the steps:
1) screen:Select complete full isatis root seed;
2) acidification:By step 1) screening isatis root seed be with mass fraction 0.02%~0.05% hydrochloric acid or acetic acid soak 8~10 hours;
3) freezing processing:Treat step 2) the isatis root seed of acidification is placed in refrigerating chamber, Adjust temperature and be adjusted to -20 DEG C from the speed that room temperature starts to lower 2 DEG C daily, 10 are freezed afterwards My god, then adjust temperature and be adjusted to room temperature from -20 DEG C of speed started to raise 2 DEG C daily;
4) drought stress:By step 3) process isatis root seed be placed on air permeable Room-dry between 60%~80%, obtains final product the isatis root seed of pretreatment to water content.
CN201510736423.4A 2015-11-03 2015-11-03 Pre-treatment method of isatis root culture Pending CN106612704A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510736423.4A CN106612704A (en) 2015-11-03 2015-11-03 Pre-treatment method of isatis root culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510736423.4A CN106612704A (en) 2015-11-03 2015-11-03 Pre-treatment method of isatis root culture

Publications (1)

Publication Number Publication Date
CN106612704A true CN106612704A (en) 2017-05-10

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Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1424886A1 (en) * 2001-09-12 2004-06-09 Virens Di Baggio Nicola & Andrea S.N.C. Item for the cultivation of an agricultural plantation and method for making said item
CN101218872A (en) * 2008-02-01 2008-07-16 广州白云山和记黄埔中药有限公司 Isatis root cultivation method
CN103583204A (en) * 2013-10-23 2014-02-19 张小友 Fruit tree and Chinese medicinal herb interplanting method
CN103828512A (en) * 2014-03-18 2014-06-04 天津科润农业科技股份有限公司 Method for breaking dormancy of new celery seeds
CN103975748A (en) * 2014-05-29 2014-08-13 太仓市惠勤农场专业合作社 Isatis root planting method
CN104041260A (en) * 2013-03-14 2014-09-17 江南大学 Planting method of radix isatidis
CN104541900A (en) * 2015-01-20 2015-04-29 蒋世芬 Planting method of peas
CN104798487A (en) * 2015-04-17 2015-07-29 山东省分析测试中心 Method for promoting radix isatidis seed germination
CN104855269A (en) * 2015-04-03 2015-08-26 浙江省嘉兴市农业科学研究院(所) Method for breeding multiple generations of paddy rice in one year

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1424886A1 (en) * 2001-09-12 2004-06-09 Virens Di Baggio Nicola & Andrea S.N.C. Item for the cultivation of an agricultural plantation and method for making said item
CN101218872A (en) * 2008-02-01 2008-07-16 广州白云山和记黄埔中药有限公司 Isatis root cultivation method
CN104041260A (en) * 2013-03-14 2014-09-17 江南大学 Planting method of radix isatidis
CN103583204A (en) * 2013-10-23 2014-02-19 张小友 Fruit tree and Chinese medicinal herb interplanting method
CN103828512A (en) * 2014-03-18 2014-06-04 天津科润农业科技股份有限公司 Method for breaking dormancy of new celery seeds
CN103975748A (en) * 2014-05-29 2014-08-13 太仓市惠勤农场专业合作社 Isatis root planting method
CN104541900A (en) * 2015-01-20 2015-04-29 蒋世芬 Planting method of peas
CN104855269A (en) * 2015-04-03 2015-08-26 浙江省嘉兴市农业科学研究院(所) Method for breeding multiple generations of paddy rice in one year
CN104798487A (en) * 2015-04-17 2015-07-29 山东省分析测试中心 Method for promoting radix isatidis seed germination

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
罗光明等: "《药用植物栽培学》", 31 January 2013, 上海科学技术出版社 *
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Application publication date: 20170510